Joint marker for diagnosing bladder cancer, kit and application

A kit and marker technology, applied in the field of bladder cancer kits and systems, can solve the problems of complex diagnosis of bladder cancer and low sensitivity

Active Publication Date: 2019-05-03
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the clinical problems of complex diagnosis of bladder cancer and low sensitivity, to provide an application of a combination of small molecule metabolites in the auxiliary diagnosis of bladder cancer, and to provide an analysis and detection method for the combination of small molecule metabolites

Method used

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  • Joint marker for diagnosing bladder cancer, kit and application
  • Joint marker for diagnosing bladder cancer, kit and application
  • Joint marker for diagnosing bladder cancer, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Serum Sample Collection

[0068] Before collection, all volunteers included in the study signed the informed consent.

[0069]96 cases of bladder cancer patients and 49 cases of normal control group were fasted overnight the day before, and blood was collected the next morning. The collected blood samples were left to stand for 30 minutes, centrifuged at 4500rpm / min at 4°C for 15 minutes, and the serum was collected and stored in a -80°C refrigerator for later use.

[0070] 2. Analysis method

[0071] 2.1 Serum sample pretreatment

[0072] The blood sample was thawed at 4°C, and 100 μL of serum sample was taken, and 400 μL of the extraction solution containing the internal standard (that is, containing the internal standard 1- 13 Methanol solution of C-valine, D4-chenodeoxycholic acid, D3-palmitic acid, see Table 2 for the concentration of internal standard) Precipitate protein; after shaking, centrifuge at 14000g, 4°C for 15 minutes; take 400μL of supernatant and...

Embodiment 2

[0085] Another batch of serum samples was collected to validate the combined markers of bladder cancer, including 32 patients with bladder cancer and 17 normal controls.

[0086] 1. Serum Sample Collection

[0087] The sample collection method is the same as in Example 1.

[0088] 2. Analysis method

[0089] 2.1 The serum sample pretreatment method is the same as that in Example 1.

[0090] 2.2 Ultra-high performance liquid chromatography mass spectrometry analysis

[0091] The liquid phase and mass spectrometry conditions are the same as in Example 1.

[0092] 2.3 Verification results.

[0093] The contents of choline alfoscerate, cystine, dodecenoic acid, eicosenoic acid, and chenodeoxycholic acid were significantly different in the bladder cancer patient group and the normal control group. Using the binary logistic regression equation, when the cut-off value of 0.500 is used for the combined markers, the results are shown in Table 4 below.

[0094] Table 4.

[0095] ...

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Abstract

The invention relates to a new application of small molecule metabolites of serum samples, such as choline glycophosphate, cystine, dodecanoic acid, eicosanoic acid and chenodeoxycholic acid taken asa combined marker in the preparation of a kit for diagnosis of the bladder cancer of a subject. The invention further relates to the kit for detecting the bladder cancer of the subject, a joint markervariable is calculated based on a binary logistic regression equation by detecting respective concentrations of the above-described combined marker in the serum samples from the subject, based on thedetermined cut-off value, whether the subject has the bladder cancer is determined. The kit has advantages of high sensitivity and high detection efficiency, the small molecule metabolites have properties of low detection cost and good repeatability, and the small molecule metabolites are combined for use and can be applied to auxiliary diagnosis of the bladder cancer.

Description

technical field [0001] The present invention relates to novel uses of known metabolites. Specifically, the present invention relates to the combined use of choline alfoscerate, cystine, dodecenoic acid, eicosenoic acid and chenodeoxycholic acid in serum as a marker for the diagnosis of bladder cancer, and to the detection of Kits and systems for bladder cancer in patients. Background technique [0002] Bladder cancer (BC) is the second most common human malignancy affecting the urinary system and one of the deadliest cancers worldwide, with its morbidity and mortality increasing gradually in recent decades. The pathogenesis and development mechanism of bladder cancer has not yet been elucidated, and its risk factors involve the combined effects of polygenic inheritance, environmental factors and lifestyle. Early detection of bladder cancer is the key to improving the survival rate of patients, the survival rate of early patients is as high as 95%; however, the survival rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 许国旺郑思佳赵欣捷王晴晴王晓琳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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