65 results about "Choline Phosphate" patented technology

The present disclosure relates to nutritional compositions comprising a neurologic component, wherein, the neurologic component may promote brain and nervous system development and further provide neurological protection and repair. The neurologic component may include phosphatidylethanolamine, sphingomyelin, cytidine diphosphate-choline, ceramide, uridine, at least one ganglioside, and mixtures thereof. The disclosure further relates to methods of promoting brain and nervous system health by providing said nutritional compositions to target subjects, which includes pediatric subjects.

The present invention is directed to a method of treating patients with major depression by administering omega-3 fatty acids. These may be administered in a substantially purified form, as part of a pharmaceutical composition, or as part of a larger molecule, e.g., a triacylglycerol, which releases free fatty acid after ingestion by a patient.The present invention is also directed to triacylglycerols which are esterified at the gamma cardon of glycerol to phosphocholine and at either the alpha or beta carbon of glycerol to an omega-3 fatty acid. These “omega-3 phoshatidylcholines” are also used in the treatment of patients with major depression.

The invention relates to a medical instrument field, in particular to a medicine coating layer of blood vessel support of degradation polymer with anti-coagulant phosphoric acid choline ingredient. The medicine coating layer is formed by 1 percent to 50 percent medicine and medicine carrier of 50 percent to 99 percent degradation polymer with anti-coagulant phosphoric acid choline ingredient. The medicine can choose individual oxidation resisting medicine, mixture of diverse oxidation resisting medicines, mixture of single or diverse blood vessel restenosis medicines, mixture of single or diverse oxidation resisting medicine and blood vessel restenosis medicine composition. The medicine carrier is a copolymer of three macromolecules of polylactic acid (PDLLA), polyethylene glyco and phosphoric acid choline. And the medicine and medical biological degradation polymer are coated uniformly on the surface of the blood vessel support by the mixing or stratification coating method. According to local medication, the thrombus incidence rate can be effectively eased and reduced, so that the restenosis of coronary artery can be finally cured.

The invention relates to a preparation method of L-alpha-glycerophosphoryl choline. The preparation method comprises the following steps of: firstly, condensing (R)-epoxy chloropropane with benzyl alcohol in the presence of strong base to prepare (S)-benzyl glycidyl ether; then enabling the (S)-benzyl glycidyl ether to react with choline phosphate chloride to obtain L-alpha-choline phosphate chloride benzyl ether; then removing the benzyl protection radial from the obtained L-alpha-choline phosphate chloride benzyl ether by Pd / C hydrogenation reaction, purifying, so as to obtain the L-alpha-glycerophosphoryl choline (GPC) finally. By adopting the cheap and easily available chiral epoxy chloropropane as the original raw material, the side reaction is avoided; and furthermore, the purity of product is good and the yield is high.

The present invention relates to a method, which comprises that (R)-(-)-3-chloro-1,2-propanediol and a phosphocholine tetramethyl ammonium salt are subjected to a substitution reaction, and ion exchange resin purification is performed to obtain a L-alpha-choline glycerophosphate pure product. According to the present invention, the used chiral intermediate (R)-(-)-3-chloro-1,2-propanediol is further a key chiral intermediate of an antitussive agent levodropropizine, has characteristics of stable chemical property and convenient and easy obtaining, and is especially for L-alpha-choline glycerophosphate industrial production.

The invention relates to the field of medicines, in particular to a composition containing choline alfoscerate. The composition is characterized by being prepared from the following components: 5-20 percent of choline alfoscerate, 0-5 percent of glycerin, a suitable amount of other accessories and the balance of injection water; the content and the impurities of the injection are detected by adopting a high-pressure liquid-phase chromatogram; and the total amount of the impurities is controlled to be not more than 2 percent. The choline alfoscerate is clinically used for treating patients with senile dementia and cerebral apoplexy.

The present invention relates to a production process of citicoline. It is characterized by that said production process includes the following steps: firstly, adding water and glucose into a reaction tank, then adding yeast, making fermentation, after fermentation adding inorganic salt and choline phosphate, the adding cytidine monophosphate solution, an adding cane sugar, heating, then quickly-cooling by using water, pressing reaction liquid, washing by using water, removing salt from obtained clear liquor; then pressing said clear liquor and making said clear liquor be fed into a carbon column, rinsing said carbon column by using pure water, then using ethyl alcohol solution to make elution, collecting citicoline sodium; then vaporizing and concentrating eluent, diluting concentrate and making it be fed into a macroporous ion exchange resin column, then washing said column by using water and making elution, concentrating eluent, heating the collected citicoline sodium solution, removing impurity by utilizing ultrafiltration device, decolouring, adding ethyl alcohol and stirring them, storing the above-mentioned material in a refrigeration house and staying overnight; then making crystal solution undergo the processes of centrifugal separation, vacuum drying, bagging, weighing, sealing and dry-storage.

The present invention relates to one kind of compound and its preparation process. The compound is designed for inhibiting the function of C-reactive protein likely to cause atherosclerosis and myocardial infarction so as to prevent and treat cardiac vascular diseases. By using ligustrazine derivative 2, 5-dihydroxymethyl-3, 6-dimethyl pyrazine as precursor and through a series of reaction, compound 2, 5-bis(choline phosphate) methyl-3, 6-dimethyl pyrazine capable of inhibiting the activity of C-reactive protein is prepared.

The invention discloses a method for preparing citicoline through biological enzyme catalysis. The method is characterized by comprising the steps that a single artificially-transformed gene engineering strain is used as an enzyme source, an ammonium chloride catalyst, orotic acid, choline phosphate and other chemical substances are subjected to a reaction to generate citicoline, and then the citicoline is extracted from the reaction solution. Compared with the prior art, single strain is adopted for fermentation, the production technology is simple, the production period is short, the production cost is low, and the method for preparing citicoline through biological enzyme catalysis can be widely applied to industrial production of citicoline.

The invention relates to Issatchenkia orientalis and a method for producing citicoline by whole cell conversion of Issatchenkia orientalis, belonging to the technical field of biological pharmacy. In the method provided by the invention, the whole cells of Issatchenkia orientalis Z1, namely CCTCC (China Center for Type Culture Collection) NO: M2011272 are utilized to prepare citicoline, choline phosphate and 5'-cytidylic acid are used as substrates, glucose is used as an energy donor, and the ATP (adenosine triphosphate) regeneration efficiency is improved by adding inorganic ions; glucose is used as the energy donor, so that the energy requirement of the strain is provided and ATP is provided for a citicoline synthesis enzyme system; one or more of potassium ion, magnesium ion and manganese ion are added to change the metabolism flow direction and improve the ATP regeneration rate, so that the ATP regeneration rate is matched with the rate of the citicoline enzyme synthesis system and the high-efficiency preparation of citicoline is achieved; and the whole cells of Issatchenkia orientalis are used, and toluene is added to an aqueous solution during the preparation process so as to improve the cell permeability, so that the rate of the citicoline enzyme synthesis system is improved.

The invention discloses a biomimetic amphiphilic graft copolymer with a cell membrane structure. The main chain of the copolymer is chitosan, and the side chains are hydrophilic choline phosphate and hydrophobic aliphatic acid. The prepared amphiphilic graft copolymer has the biomimetic characteristics of cell membrane in structure; the choline phosphate molecules mimic the head parts of phospholipid molecules in cell membrane, the sugar units of the chitosan mimic the head parts of the glycolipid molecules in the cell membrane, and the hydrophobic aliphatic acid mimic the hydrophobic carbon chains of the phospholipid molecules and the glycolipid molecules. The copolymer has very good biological compatibility and hydrophilicity. When the copolymer is taken as the nano drug carrier, the copolymer can resist the non-specific adhesion of proteins, and can be easily swollen by the focal cells. Moreover, the graft copolymer can be evenly and stably dispersed in a solution with a high salinity and wide pH range. The invention further provides a preparation method of the copolymer at the same time. The preparation method has the advantages of simple operation, low cost, mild reaction conditions, and high yield.

The invention provides a bioengineering method for synthesis of citicoline. The method comprises the following steps: constructing an escherichia coli engineering strain containing choline phosphate cytidylyltransferase by recombination through a genetic engineering method; utilizing the engineering strain to perform large-scale high-density culture as well as separation and purification, thereby obtaining the choline phosphate cytidylyltransferase with high purity and high activity; utilizing the choline phosphate cytidylyltransferase to perform enzymatic catalysis synthesis under appropriate conditions to obtain the citicoline; and adding cation exchange resin into a system of the enzymatic catalysis synthesis of the citicoline to enable the citicoline to be constantly adsorbed on the resin in the synthesis process, thereby realizing coupling of reaction and separation of the citicoline. The bioengineering method has the advantages that the substrate utilization rate is high, the product recovery rate is high, reaction conditions are mild, the reaction time is short, the environment pollution is small, the cost is low, and the like, thus being suitable for industrial mass production.

The invention relates to a new application of small molecule metabolites of serum samples, such as choline glycophosphate, cystine, dodecanoic acid, eicosanoic acid and chenodeoxycholic acid taken asa combined marker in the preparation of a kit for diagnosis of the bladder cancer of a subject. The invention further relates to the kit for detecting the bladder cancer of the subject, a joint markervariable is calculated based on a binary logistic regression equation by detecting respective concentrations of the above-described combined marker in the serum samples from the subject, based on thedetermined cut-off value, whether the subject has the bladder cancer is determined. The kit has advantages of high sensitivity and high detection efficiency, the small molecule metabolites have properties of low detection cost and good repeatability, and the small molecule metabolites are combined for use and can be applied to auxiliary diagnosis of the bladder cancer.

The invention discloses a composition for improving photosynthetic efficiency. The composition mainly comprises at least one choline salt and sodium bisulfate according to a weight ratio of (50: 1) to (1: 10). The at least one choline salt is selected from choline chloride, choline phosphate, choline sulfate, choline citrate, choline salicylate and choline succinate. Preferably, the choline salt is choline chloride. A weight ratio of the choline salt to sodium bisulfate in the composition is in a range of (20: 1) to (1: 10) and preferably is in a range of (5: 1) to (2: 1). When being directly used for plants, the composition can be diluted until a choline salt concentration is in a range of 40 to 1000mg / L and a sodium bisulfate concentration is in a range of 10 to 800mg / L, and preferably, the composition can be diluted until a choline salt concentration is in a range of 300 to 1000mg / L and a sodium bisulfate concentration is in a range of 50 to 120mg / L.

The present disclosure provides compositions and methods for the biosynthetic production of choline, ethanolamine, phosphoethanolamine, and phosphocholine.

The invention discloses a method for constructing a chitosan-PMCP / gelatin coating on the surface of bionic porous titanium alloy. The method comprises the steps as follows: firstly, preparing CP (choline phosphate) monomers, and modifying chitosan with PMCP; then, preparing an electrophoretic solution, and putting the electrophoretic solution into an electrophoresis container for electrophoretic deposition to obtain the porous titanium alloy with the chitosan-PMCP / gelatin coating. The porous titanium alloy with the chitosan-PMCP / gelatin coating prepared by the method has the functions of restraining non-specific protein adsorption and promoting cell adhesion; compared with single-layer CP, the PMCP is further enhanced in hydrophily, cell adhesion and resistance to non-specific protein adsorption.

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R1, R2, R3, and R4 are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

The invention discloses a method for preparing an ester and ether copolymerized viscosity-reducing polycarboxylic acid water reducing agent. The method comprises the following steps: (1) esterified monomer preparation; (2) copolymerization; and (3) neutralization. According to the method disclosed by the invention, the ester and ether copolymerized viscosity-reducing polycarboxylic acid water reducing agent is obtained by the copolymerizationan of an esterified monomer, an ester macromonomer, 2-methacryloyl ethyl phosphocholine and unsaturated acid, wherein the esterified monomer is obtained by carrying out esterification on unsaturated polyether and 2-phosphate butane-1, 2, 4-tricarboxylic acid and mainly comprises products obtained by esterifying singe carboxyl in the 2-phosphate butane-1, 2, 4- tricarboxylic acid and esterifying double and three carboxyl in small amount of 2-phosphate butane-1, 2, 4- tricarboxylic acid; and while phosphate radical, carboxylate radical, quaternary ammonium salt and ester group are introduced into a polymer molecular structure, the polymer structure is also lightly crosslinked at the same time. The ester and ether copolymerized viscosity-reducingpolycarboxylic acid water reducing agent prepared by adopting the method disclosed by the invention has the properties of viscosity reducing, water reducing, slump loss resisting and mud resisting tosolve the problems of high viscosity, poor workability and damage rapidness caused by large mud content in conventional concrete raw materials.

The invention discloses water-soluble modified chitosan, and belongs to the technical field of macromolecular compound modification. The water-soluble modified chitosan is prepared by Michael additionreaction of 2-methacryloyloxyethyl choline phosphate and chitosan, the specific structure is that the modified chitosan can be applied to the preparation of skin wound dressing, drug carriers and antibacterial coating agents. The modified chitosan is soluble in water and has good water solubility, the use of an acid solution is avoided, side effects are reduced, and environmental pollution is reduced; reaction is only conducted on the amino group of the chitosan, an original skeleton of the chitosan is retained, and the original physical and chemical activity of the chitosan cannot be lost; the 2-methacryloyloxyethyl choline phosphate is introduced, and more excellent biological property is achieved on the chitosan; and the preparationmethod of the modified chitosan is simple and easy tooperate, the experimental conditions are mild, the yield of finished products is high, the water-soluble modified chitosan is of great guiding significance for industrial production and application, and is suitable for wide application and popularization.

The invention belongs to the technical field of crop planting, and particularly relates to a citrus reticulata blanco planting method. The planting method comprises the following steps: (1) garden construction and land preparation: digging planting holes, and digging out soil for later use; (2) planting of seedlings: uniformly mixing the dug-out soil with fertilizer special for citrus reticulata blanco, thereby obtaining mixed soil for planting the citrus reticulata blanco seedlings, wherein the fertilizer special for the citrus reticulata blanco is prepared from enteromorpha polysaccharides, peanut bran, calcium superphosphate, animal dung, rice straws, potassium nitrate, urea, a spanish bayonet extract, coral dealbatus, pyrethrum flowers, choline phosphate, smilax perfoliata lour, biogas residues and micro element fertilizer; (3) fertilizer and water management for young trees; (4) fertilizer and water management for resulting trees; (5) daily management. The planting method can improve the soil, promote rapid and healthy growth of the citrus reticulata blanco seedlings, increase the yield and shorten the resulting period.

The invention discloses a synthetic method of citicoline sodium. The synthetic method comprises the following steps: adding 5'-cytidylic acid, calcium phosphorylcholine chloride, ATP, an aqueous magnesium chloride solution and a Tris-HCl buffer solution into a reaction vessel, and carrying out uniform mixing; then, adjusting a pH value to 6.0-8.0, adding CMP phosphokinase, nucleoside diphosphate kinase and choline phosphate-cytidyltransferase, and carrying out a stirring reaction for 4-10 hours at a temperature of 25-50 DEG C; and conducting high-speed centrifugation, and taking a supernatantto obtain citicoline sodium. Compared with the traditional citicoline sodium synthesis process, the synthetic method provided by the invention is environment-friendly in a synthesis route, stable in yield and is beneficial for reducing energy consumption and protecting the environment, so the synthetic method is suitable for large-scale industrial production.

The invention discloses a water-soluble cyclodextrin drug carrier with cell targeting. The water-soluble cyclodextrin drug carrier comprises beta-cyclodextrin as an entrapment drug body, and the beta-cyclodextrin is linked with at least one choline phosphate at a fixed point. The preparation method comprises the following steps: (1) taking methanol and 2-chloro-2-oxo-1, 3, 2-dioxaphospholane as raw materials to synthesize 2-methoxy-2-oxo-1, 3, 2-dioxaphospholane; (2) synthesizing choline phosphate by taking 2-methoxy-2-oxo-1, 3, 2-dioxaphospholane and 1-dimethylamino-2-propyne as raw materials; and (3) finally, through a click reaction, enabling the mono (6-azide-6-deoxy)-beta-cyclodextrin, choline phosphate and a ligand N, N, N', N, N''-pentamethyldiethylenetriamine to react to generate the mono-substituted choline phosphate cyclodextrin. The drug carrier can improve the hydrophilicity of beta-cyclodextrin and endow the beta-cyclodextrin with cell adhesiveness by utilizing the chargeeffect between choline phosphate and a cell membrane, so that the high efficiency of drug absorption in cells is realized.

The invention particularly relates to preparation of amphoteric ion modified hydrophilic carbon spheres, and applications of the amphoteric ion modified hydrophilic carbon spheres in glycopeptide enrichment. According to the invention, glucose is used as a carbon source, a choline phosphate type monomer is used as a functional reagent to provide zwitterions, and carbon spheres with a particle sizeof 200-400 nm and hydrophilic surfaces are prepared through a hydrothermal method, wherein the preparation process of the hydrophilic carbon spheres is simple, the reaction conditions are mild, and the used raw materials are environmentally friendly; and the enrichment performance of the zwitterionic hydrophilic carbon spheres on glycopeptides is inspected by using a standard glycoprotein IgG enzymatic hydrolysate, and the zwitterionic hydrophilic carbon spheres are further applied to glycoproteomics analysis in serum.

The invention discloses a dithiophospholipid compound and a preparation method thereof. The preparation method comprises the following steps: weighing cystamine hydrochloride and triethylamine absolute methanol, adding di-tert-butyl dicarbonate, and reacting to obtain single protection cystamine; weighing fatty acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxythiosuccinimide, dissolving in dichloromethane, adding mono-protected cystamine, mixing, dissolving reactants in dichloromethane, and adding trifluoroacetic acid to obtain a solid; dissolving the solid in dichloromethane, adding triethylamine and succinic anhydride, and reacting to obtain an intermediate product; and weighing the intermediate product according to the ratio, dissolving dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dimethyl sulfoxide, activating, adding choline glycerophosphate, and reacting to obtain the target product. The structure of the dithiophospholipid compound provided by the invention contains a disulfide bond which is easily reduced by glutathione, so that phospholipid is broken in the presence of GSH, and the dithiophospholipid compound has a GSH response breaking function.

The invention belongs to the field of biomedical materials and particularly relates to a method for preparing a polymer liquid crystal membrane material with a biomimic structure and a use of the material. The method for preparing the polymer liquid crystal membrane material with the biomimic structure comprises the following steps: coating the polymer liquid crystal material in liquid crystal state under a physiological temperature by a solution method to form a film, immersing into the water solution of the vinyl monomer containing choline phosphate and a polymerization inhibitor to immerse, and co-radiating by <60>Co-gamma ray or electron beam to perform grafting reaction to obtain the polymer liquid crystal membrane material with the biomimic structure. The method is simple in process, low in cost, short in production period and excellent in industrial application future, the effects of energy saving and emission reduction are realized by using a civil nuclear technology, and the environment pollution is reduced; while obtaining the polymer liquid crystal membrane material with the biomimic structure, the molecular structure and the aggregation structure of the cell membrane are simultaneously simulated, and the material is excellent in surface biological property and is widely used in the fields such as the surgical dressing, the interventional implantation instrument and the medical instrument contacted with the blood.

The invention relates to an accelerant for improving the biological treatment effect of salt-containing wastewater. The accelerant mainly comprises a quaternary ammonium base and humus, and the quaternary ammonium base is at least one of choline phosphate, betaine, tetramethylammonium hydroxide and the like. The invention also provides a biological treatment method for treating high-salt wastewater by using the accelerant. The accelerator provided by the invention is simple in formula, easy to prepare, small in dosage, environment-friendly and economical. When the accelerator is used for biological treatment of salt-containing wastewater, the adaptability of a treatment system to a high-salt environment can be improved, and the treatment effect of ammonia nitrogen and total nitrogen in thesalt-containing wastewater is improved.