Ubelin manufacturing technique

A citicoline, production process technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low conversion rate and yield, yeast environmental pollution, high production cost, etc., to achieve Improve the conversion rate and yield, solve the pollution of bacteria, and reduce the production cost

Inactive Publication Date: 2008-02-27
张剑
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing citicoline production process has a low conversion rate and yield rate of the product, and the discarded yeast in the production process causes great pollution to the environment, yeast resources cannot be fully utilized, and the production cost is high
After the biochemical reaction of the product, the fermentation liquid cannot effectively remove impurities such as inorganic salts, and the purity of the product is low
The color and protein of the product are difficult to remove, the clarity of the product is not good, and there are bacteria pollution problems in the production process
The activity of the waste yeast produced by the winery is low, so that the conversion rate and yield of the product are very low, and it is difficult to meet the standard requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Feeding:

[0016] 1. Add tap water and glucose into the reaction tank, then raise the temperature to 41°C, put in yeast, stir well, let it ferment for 4 hours, then add inorganic salt and phosphorylcholine, when the temperature rises to 30°C, put in cytidylic acid solution, and then continue to heat up to 40 ℃, heat preservation timer.

[0017] 2. The reaction temperature is controlled at 40°C. After 3 hours, take a sample and send it to the analysis room to measure the conversion rate, and then add sucrose, the amount of which is half of that when feeding. After that, add sucrose every two hours. When the conversion rate reaches The reaction can be terminated when it is above 80%, and the reaction time can be prolonged if it is lower than 70%.

[0018] 3. After the reaction is completed, heat up to 85°C, quickly cool down to room temperature with water circulation, and then send the reaction liquid to a press for pressing.

[0019] 4. After pressing, top wash with...

Embodiment 2

[0039] 1. Feeding:

[0040] 1. Add tap water and glucose into the reaction tank, then raise the temperature to 40°C, put in yeast, stir well, let it ferment for 3 hours, then add inorganic salt and phosphorylcholine, when the temperature rises to 29°C, put in cytidylic acid solution, and then continue to heat up to 39 ° C, heat preservation timer.

[0041] 2. The reaction temperature is controlled at 39°C. After 2.5 hours, take a sample and send it to the analysis room to measure the conversion rate, and then add sucrose, the amount of which is half of that when feeding. After that, add sucrose once every two hours. When the conversion rate reaches The reaction can be terminated when it is above 80%, and the reaction time can be prolonged if it is lower than 70%.

[0042] 3. After the reaction is completed, heat up to 80°C, quickly cool down to room temperature with water circulation, and then send the reaction liquid to a press for pressing.

[0043] 4. After pressing, top ...

Embodiment 3

[0063] 1. Feeding:

[0064] 1. Add tap water and glucose into the reaction tank, then raise the temperature to 42°C, put in yeast, stir well, let it ferment for 5 hours, then add inorganic salts and phosphorylcholine, when the temperature rises to 31°C, put in cytidylic acid solution, and then continue to heat up to 41 ° C, heat preservation timer.

[0065] 2. Control the reaction temperature at 41°C. After 3.5 hours, take a sample and send it to the analysis room to measure the conversion rate, and then add sucrose, the amount of which is half of that when feeding. After that, add sucrose once every two hours. When the conversion rate reaches The reaction can be terminated when it is above 80%, and the reaction time can be prolonged if it is lower than 70%.

[0066] 3. After the reaction is completed, heat up to 90°C, quickly cool down to room temperature with water circulation, and then send the reaction liquid to a press for pressing.

[0067] 4. After pressing, top wash ...

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PUM

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Abstract

The present invention relates to a production process of citicoline. It is characterized by that said production process includes the following steps: firstly, adding water and glucose into a reaction tank, then adding yeast, making fermentation, after fermentation adding inorganic salt and choline phosphate, the adding cytidine monophosphate solution, an adding cane sugar, heating, then quickly-cooling by using water, pressing reaction liquid, washing by using water, removing salt from obtained clear liquor; then pressing said clear liquor and making said clear liquor be fed into a carbon column, rinsing said carbon column by using pure water, then using ethyl alcohol solution to make elution, collecting citicoline sodium; then vaporizing and concentrating eluent, diluting concentrate and making it be fed into a macroporous ion exchange resin column, then washing said column by using water and making elution, concentrating eluent, heating the collected citicoline sodium solution, removing impurity by utilizing ultrafiltration device, decolouring, adding ethyl alcohol and stirring them, storing the above-mentioned material in a refrigeration house and staying overnight; then making crystal solution undergo the processes of centrifugal separation, vacuum drying, bagging, weighing, sealing and dry-storage.

Description

Technical field: [0001] The invention relates to a production process of chemical products, which is a citicoline production process for treating waste yeast produced in a brewery. Background technique: [0002] In the existing citicoline production process, the conversion rate and yield of the product are low, the discarded yeast in the production process causes great pollution to the environment, the yeast resources cannot be fully utilized, and the production cost is high. After the biochemical reaction of the product, the fermentation broth cannot effectively remove impurities such as inorganic salts, and the product has low purity. The color and protein of the product are difficult to remove, the clarity of the product is not good, and there are bacteria pollution problems in the production process. The activity of the strains of the waste yeast produced by the winery is low, so that the conversion rate and yield of the product are very low, and it is difficult to meet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P9/00C12R1/85
Inventor 张剑
Owner 张剑
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