The invention discloses a preparation method of Radix Puerariae wine. The preparation method comprises the following steps: collecting fresh Radix Puerariae, cleaning, cutting into pieces, adding water, and performing homogenate to obtain a Radix Puerariae homogenate liquid; sterilizing the Radix Puerariae homogenate liquid at 70-110 DEG C for 5-120 minutes, cooling to 50-65 DEG C, and regulatingthe pH value to 5-7 to obtain a sterilized solution; adding amylase into the sterilized solution until the mass percentage of the amylase is 0.01-5, performing enzymolysis at 50-90 DEG C for 0.5-24 hours, adding glucoamylase until the mass percentage of the glucoamylase is 0.01-5, and performing enzymolysis at 50-70 DEG C for 4-12 hours; then increasing the temperature to 70-110 DEG C, holding the temperature for 20-150 minutes, and cooling to 20-60 DEG C to obtain an enzymolysis solution; and finally adding saccharomyces cerevisiae into the enzymolysis solution until the mass percentage of the saccharomyces cerevisiae is 0.01-0.05, fermenting at 24-40 DEG C for 5-10 days, and filtering to obtain the Radix Puerariae wine. The preparation method is simple; and the prepared Radix Puerariae wine is high in Puerarin content and has an alcohol degree of 15-35, and can be applied to adjuvant therapy for diseases such as hypertension, angina pectoris, coronary heart disease, hyperlipidemia and the like.