8 results about "Amino acid solution" patented technology

The application belongs to the field of biochemical synthesis instruments, and discloses a 96-channel synthesis instrument and a control method thereof. The instrument comprises a body, a synthesis operation cavity and a reagent storage and transportation cavity which are arranged in layers and are isolated from each other inside the body, a mechanical arm and a pipettor which are capable of multi-axis movement are arranged in the synthesis operation cavity, and amino acid bottles are arranged in an array. The core structure is a rotary feeding mechanism, 96 reactors which are uniformly distributed in a circle are arranged on the periphery of a rotary feeding disc of the rotary feeding mechanism through quick release mechanisms, a liquid adding tower is fixed above a corresponding liquid adding station, and the liquid adding tower is used for adding solvents or activating reagents to the reactors in a quantitative manner. The mechanical arm is responsible for the preparation and transfer of amino acid solutions, and the liquid adding tower is responsible for batch solvent treatment. The rotary feeding mechanism, the mechanical arm and the liquid adding tower are cooperated to realize efficient division of labor and parallel synthesis. The application has a compact structure, and the design of combination of rotary feeding and fixed liquid adding significantly improves the synthesis efficiency, operation stability and maintenance convenience.

This invention provides an amino acid-activated ice capable of easily and quickly generating gas hydrates, a method for generating the same, and a gas hydrate using amino acid-activated ice, as well as a method for generating the same. [Solution] The amino acid-activated ice is made by freezing an aqueous amino acid solution containing hydrophobic amino acids and dissolved CO2 gas. The concentration of amino acids in the aqueous amino acid solution containing hydrophobic amino acids may be in the range of 0.01 wt% to 2.0 wt%. The hydrophobic amino acid may be any one of leucine, methionine, glycine, phenylalanine, tryptophan, and histidine.

The application belongs to the technical field of cosmetics, and particularly relates to a preparation method of a small molecule polypeptide of a leaf-eating plant and an amino acid solution and application thereof. The method obtains specific peptide segments by gradually enzymatically digesting proteins contained in the leaf-eating plant and by controlling pH values and enzyme types, so as to realize efficient enzymatic digestion and improve the purity of products, thereby laying a foundation for the application of the products in the field of cosmetics. The method of the application not only has safe and reliable production principles, but also improves the yield of the small molecule polypeptide of the leaf-eating plant, and solves the technical problem that cosmetic protein raw materials are difficult to penetrate the skin.

The present application belongs to the technical field of cosmetics, and particularly relates to an anti-aging essence containing small molecule polypeptide of food leaf and amino acid. The essence realizes the effects of antioxidation, promotion of cell proliferation and improvement of skin state by adding the small molecule polypeptide of food leaf and amino acid freeze-dried powder. The small molecule polypeptide and amino acid freeze-dried powder is prepared from a small molecule polypeptide and amino acid solution through freeze-drying. The small molecule polypeptide and amino acid solution is obtained by gradually enzymatically hydrolyzing the protein contained in food leaf, controlling the pH value and the type of enzyme, and obtaining specific peptide segments, so as to realize efficient enzymatic hydrolysis, obtain specific peptide segments and improve the purity of the product. Through enzymatic hydrolysis in different stages, not only the safe and reliable production principle is provided, but also the yield of the small molecule polypeptide of food leaf is improved, and the technical problem that the protein raw material of cosmetics is difficult to penetrate the skin is solved.

The application belongs to the technical field of polypeptide synthesis equipment, and discloses a multi-channel synthesizer and a synthesis method thereof. The synthesizer comprises a body, a synthesis operation cavity and a reagent storage and transportation cavity are independently arranged in the body through physical isolation, a reaction system comprising a plurality of independent swing reactors, a common metering and transferring unit, and a corresponding number of activation bottles corresponding to the reactors are arranged in the synthesis operation cavity, and an amino acid solution supply unit is arranged at the upper portion; reagent storage and transportation cavities are centrally arranged with DMF, DCM, piperidine, condensation reagent, methanol and other storage bottles, two independent nitrogen positive pressure conveying paths are adopted: the first path is connected with amino acids, condensation reagents, a metering activation system and a reaction system, and is specially used for precise metering, mixing and adding of the condensation reaction; the second path is connected with various types of washing and deprotection reagent storage bottles and the reaction system, and is used for direct and rapid adding. The synthesis process is optimized, the cross contamination and reagent evaporation risk are significantly reduced, and the efficiency and reliability of multi-channel parallel synthesis are improved.

The present application relates to the technical field of amino acid detection, and specifically relates to a method for quantitatively detecting free amino acids in a polypeptide drug starting material protective amino acid, wherein the liquid chromatography conditions are as follows: the chromatographic column is AQ-C18, the mobile phase A is 0.1+ / -0.02% formic acid aqueous solution, the mobile phase B is 0.1+ / -0.02% formic acid acetonitrile solution, and the diluent is acetonitrile aqueous solution; the CAD detector conditions are as follows: the evaporation temperature is 50+ / -2 DEG C, and the gas adjustment mode is the analysis mode; and the free amino acid content is calculated by substituting the peak area of the amino acid reference solution and the peak area of the Fmoc protective amino acid solution into the external standard method. Compared with the pre-column derivatization-high performance liquid chromatography method, the present application does not need expensive derivatization reagents and complex derivatization processes; compared with the ultra-high performance liquid chromatography-tandem mass spectrometry method, the present application does not need to configure expensive mass spectrometers, has good economic efficiency and practical value, and can quantitatively detect the free amino acid content.

The application relates to the technical field of nanobiological sensing, and discloses D-type amino acid modified gold nanoparticles as well as a preparation method and application thereof, which comprises the following steps: (1) dissolving D-type amino acid in deionized water to obtain a D-type amino acid solution, adding anhydrous acetic acid, a HAuCl4 solution and Tween-80 into the D-type amino acid solution, uniformly mixing, boiling at 100 DEG C, and keeping boiling; under the condition of violent stirring, adding a sodium citrate aqueous solution into the solution prepared in step (1), and continuously keeping boiling; after the reaction is completed, cooling to room temperature; using deionized water to dialyze the solution prepared in step (3), filtering and sterilizing, and obtaining the D-type amino acid modified gold nanoparticles. The D-type amino acid modified gold nanoparticles can detect bacteria through the color change of a solution, realize visual detection of bacteria, and do not need to rely on large instruments.