355 results about "Cystine" patented technology

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The invention relates to the technical field of functional microbial preparations, in particular to a biological preparation for relieving intestinal discomfort caused by alcohol and a preparation method and application thereof.The main technical scheme is that the biological preparation is prepared from, by weight, 5-20 parts of bacillus coagulans, 10-30 parts of pediococcus pentosaceus, 5-10 parts of lactobacillus rhamnosus and 0-20 parts of saccharomycetes. 0-5 parts of bifidobacterium longum and 0-5 parts of lactococcus lactis. By constructing an alcohol metabolism-intestinal protection dual-function synergistic system, specific florae such as bacillus coagulans, pediococcus pentosaceus and lactobacillus rhamnosus are scientifically compatible with functional components such as inulin and L-cysteine, alcohol is efficiently metabolized, and a carbon source is generated; the technical problems of low metabolic efficiency, weak intestinal protection and serious gastric acid inactivation of a traditional product are comprehensively solved.

The invention relates to the technical field of agriculture, in particular to a zinc-rich cadmium-reducing leaf surface blocking and controlling agent as well as a preparation method and an application method thereof. According to the specific technical scheme, the zinc-rich cadmium-reducing leaf surface blocking and controlling agent is prepared from, by mass, 10%-20% of gamma-chitosan, 20%-30% of L-malic acid and 50%-70% of zinc sulfate. The technical problem that in the prior art, the cadmium content of brown rice cannot be reduced through a leaf surface blocking and controlling agent is solved.

Provided a method for promoting growth of bacteria of the genus Bifidobacterium. The method for promoting growth of bacteria of the genus Bifidobacterium includes subjecting a milk culture medium containing one or more selected from the group consisting of cysteine, cystine, and a salt thereof to heat treatment and then culturing bacteria of the genus Bifidobacterium in the milk culture medium.

The present application relates to a kind of method and kit for detecting methylation cysteine total amount, belong to the field of biotechnology and analytical test.Utilize nucleic acid aptamer with high response to free Hcy, with moderate corresponding state Hcy to construct evanescent wave optical fiber sensor.Complementary strand and tHcy of fluorescently labeled are combined with the nucleic acid aptamer fixed on optical fiber with competition.Fluorescence signal drop value is positively correlated with tHcy concentration in serum.According to the percentage of fluorescence reduction, the tHcy concentration in serum is calculated.The present application detects tHcy only needs 1 microliter serum, after diluting 1000 times, detection is carried out, and the detection result is consistent with commercial enzyme cycle method.Compared with enzyme cycle method, the present application has the advantages of low price, optical fiber can be reused more than 30 times, detection speed is fast, fastest 5 minutes, does not need enzyme, equipment miniaturization, simple operation, etc., has excellent clinical application value.

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

The present invention provides a method for detecting the presence of or risk of bladder cancer in a female patient, the method comprising the steps of: detecting the presence of a biomarker panel in a sample isolated from a female patient, the biomarker panel comprising IL-13 and IL-12p70 and one or more biomarkers selected from the group consisting of BTA, midkine, PAI-1 / tPA, 8OHdG, CEA, CK18, Fibrillin, Creatinine, CXCL16, Cystatin B, Cystatin C, d-dimer, EGF, FAS, HAD, IL-1a, IL-1b, IL-4, IL-6, IL-7, IL-8, MCP-1, Microalbumin, MMP9 NGAL, MMP9 TIMP1, NGAL, NSE, Progranulin, TUP, TGFB1, Thrombomodulin, sTNFR1, TPA, VEGF and Triglyceride, and / or the concentration of albumin / microalbumin / protein to creatinine in a sample isolated from a female patient, expressed as an albumin:creatinine ratio; and assessing the result and comparing it to a normal control, wherein an increase in the presence of the biomarkers compared to the normal control indicates the presence or risk of cancer in the patient from which the sample was isolated.

The application discloses a modified bromhexine hydrochloride inhalation solution with enhanced stability and a preparation method thereof, and belongs to the technical field of medical preparations. The inhalation solution is mainly prepared from a targeting impurity control modified bromhexine hydrochloride inclusion compound, a special composite buffer mother liquor for inhibiting impurity B, inhalation grade sodium chloride, pharmaceutical grade sodium hydroxide and water for injection, and the pH value of the finished product is 4.5-5.5. The application constructs a three-stage progressive modification system, covalently grafts a mercapto-activated L-cysteine derivative onto hydroxypropyl-gamma-cyclodextrin to prepare a functional carrier, and realizes molecular-level isolation of bromhexine hydrochloride through host-guest inclusion, thereby blocking the generation of impurity B of bromhexine hydrochloride from the whole path, and solving the core pain points of easy impurity exceeding, short effective period and airway irritation of the existing product. The application can realize long-acting stable storage for 36 months, is suitable for the existing industrial production line, and has high clinical application and industrialization values.

The invention discloses cysteine mediated Hf / Cu bimetal synergistic self-assembled nano-particles as well as a preparation method and application of the cysteine mediated Hf / Cu bimetal synergistic self-assembled nano-particles. According to the method, cysteine is used as a multifunctional ligand, carboxyl and sulfydryl of cysteine are in bridge connection with metal through coordination, ligand chelation and assembly guiding effects are synchronously exerted, bimetallic ions are induced to be cooperatively self-assembled in an alkaline system (pH is 9-10), and metal cysteine self-assembled nanoparticles with uniform structures are formed through in-situ nucleation and multistage aggregation. According to the method, the ligand function and the bimetallic synergistic effect are accurately matched, the synthesis process is mild (room temperature), the operation is simple and convenient, and the product is excellent in dispersity and stability. Meanwhile, a close coordination structure constructed by cysteine provides a key channel for energy / electron transfer from sensitizing metal to Cu under X-ray irradiation, and lays a structural foundation for subsequent catalytic effect enhancement.

The application discloses an L-cysteine-based soil lead pollution leaching agent and a preparation method thereof, and belongs to the technical field of soil heavy metal pollution remediation. The L-cysteine-based soil lead pollution leaching agent comprises the following raw materials: an L-cysteine solution, a pH buffer and a polyvinylpyrrolidone solution; the concentration of the L-cysteine solution is 0.3-0.5 mmol / L, the concentration of the pH buffer is 1 mmol / L, and the mass fraction of the polyvinylpyrrolidone solution is 0.003%-0.005%; the volume ratio of the L-cysteine solution, the pH buffer and the polyvinylpyrrolidone solution is 1:1:0.15-0.4. The leaching agent has a remarkable leaching effect on soil lead, and the removal rate of the leaching agent on the contaminated soil with a Pb content of more than 500 mg / kg can reach more than 85%, so that the leaching agent can effectively prevent lead from migrating through the soil into a food chain or a water environment, and is much stronger than a traditional carboxyl-based leaching agent.

The invention relates to the field of antibody proteins, in particular to an immunoglobulin Fc dimer with a stable disulfide bond, an Fc fusion protein containing the Fc protein dimer and a calculation design method of the Fc fusion protein. The immunoglobulin Fc protein dimer comprises two protein subunits, a CH3 structural domain of each subunit contains at least one cysteine mutation, and the two protein subunits are combined through an interchain disulfide bond formed by mutation cysteine. Cysteine mutation is specifically introduced into the CH3 structural domain of the Fc region of the immunoglobulin to form a stable interchain disulfide bond, so that the problem of insufficient stability of the Fc protein dimer is solved.

The invention provides a method for detecting enzyme activity of papain in a hypersensitive protein complex enzyme, which comprises the following steps: mixing the hypersensitive protein complex enzyme with an L-cysteine hydrochloride buffer solution to obtain a sample solution to be detected; adding the to-be-tested sample solution into a substrate solution containing casein, and testing a change curve of a voltage value of the to-be-tested sample solution along with time under the condition that the casein can be decomposed by papain; and determining the enzyme activity of the papain in the hypersensitive protein compound enzyme according to the change curve of the voltage value of the to-be-detected sample solution along with time. The method can solve the problem that the enzyme activity of papain in the hypersensitive protein complex enzyme cannot be accurately detected in the prior art.

The application belongs to the field of biomedical application, and more particularly relates to a cell fluorescence probe anchoring cell integrin, and a preparation method and application thereof. The cell fluorescence probe comprises a polypeptide complex, a fluorescent molecule and a cyclodextrin unit, the polypeptide complex comprises a cysteine-containing peptide, a peptide containing a bonding group and a targeting peptide which are connected in any order, the cyclodextrin unit contains a carbon-carbon double bond functional group, and is combined with the cysteine-containing peptide through a click reaction to form the polypeptide complex, the polypeptide complex is covalently connected to the fluorescent molecule through the bonding group, and the fluorescent molecule is an aggregation-induced emission compound. The double bond functional group of the modified cyclodextrin is bonded to the sulfhydryl group of the terminal cysteine of the polypeptide through a click reaction, and the sulfhydryl group of the terminal cysteine of the polypeptide is bonded to the double bond functional group of the modified cyclodextrin through a click reaction, so that the cyclodextrin is improved to protect the fluorescent molecule and prolong the fluorescent time effectiveness of the fluorescent imaging reagent.

This invention belongs to the field of environmental chemistry and relates to a method for quantitatively analyzing the concentrations of cystine and cysteine ​​in a mixed solution. Based on the theoretical characteristics of the reaction between the disulfide bond in cystine, a representative amino acid, and the thiol group in cysteine, and persulfate, this invention expands the detection capability of the traditional 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB), providing support for the quantitative analysis of cystine and cysteine ​​concentrations and the study of their transformation processes.

The invention discloses a generalized pustular psoriasis diagnosis marker based on metabonomics and application of the generalized pustular psoriasis diagnosis marker. The diagnostic marker is prepared from one or more of the following 35 compounds: pyruvic acid, alpha-ketoisovaleric acid, 2-hydroxybutyric acid, 3-hydroxybutyric acid, methane thiophosphoric acid, proline, uracil, tranexamic acid, 4-aminobutyric acid, threonine, scopoletin, dodecanol, N-methyl-L-leucine, L-cysteine-glycine and L-kynurenine. The feed additive is prepared from the following raw materials: 3-hydroxybenzoic acid, allantoin, delta-tocopherol, xylofuranose, glucose-1-phosphoric acid, pyrophosphate, taurine, L-asparagine, phthalic acid, 4-(dimethylamino) azobenzene, 5-tert-butyl-1h-indole-2, 3-dione, quinic acid, glucose, histidine, lysine, palmitic acid, 7-methylguanine, oleic acid and whale acid. The marker can be used for accurately distinguishing patients with generalized pustular psoriasis from healthy people.

A process for preparing a supramolecular carrier photosensitizer for photodynamic therapy and a formulation thereof is disclosed for prevention, treatment, anti-inflammatory and sterilization of oral dental diseases. Supramolecular carrier consists of refined lignin, glucomannan, cysteine, and sodium benzoate at a mass ratio of 0.1-1.0%: 0.5-3%: 0.1-0.5%: 0.05%, and the balance of water. Aqueous solutions of refined lignin, glucomannan, cysteine and sodium benzoate are prepared, and a pH value of combined solution is adjusted to 6.5, so that dissolved alkali lignin is precipitated to form an efficient and stable three-dimensional polymer network skeleton, and rich hydrogen bonds are facilitated to be formed between the same molecules and different molecules of lignin and glucomannan, thereby forming a supramolecular carrier formulation loaded with the photosensitizer. It ensures long-term storage stability of physicochemical traits of formulation, which is conducive to rapidly promoting application of PDT in anti-inflammatory and sterilization treatment of oral dental diseases.

An O-phosphoserine (OPS) export protein variant with increased O-phosphoserine exporting activity, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the same. Also provided herein are O-phosphoserine-producing microorganisms, a polynucleotide encoding the O-phosphoserine export protein variant, and a vector containing the polynucleotide encoding the O-phosphoserine export protein variant.

The invention relates to a composite acidifying agent for improving feed intake of lactating sows and a preparation method thereof, and belongs to the technical field of feed additives. The composite acidifier comprises a wall material and a core material, wherein the wall material comprises cystine-gamma-polyglutamic acid and arginine-chitosan; the core material comprises organic acid, organic acid salt and fermentation liquor. According to the compound acidifying agent disclosed by the invention, the cystine cross-linked gamma-polyglutamic acid and the arginine grafted chitosan are used as wall materials, and the microcapsule with an intestinal targeted release function is formed by wrapping the organic acid, so that the utilization efficiency of the acidifying agent and the feed intake of lactating sows can be effectively improved.

The present invention relates to a cosmetic composition, advantageously an eco-biological cosmetic composition, comprising: - polyglutamic acid or a salt thereof; and - at least one peptide derivative represented by the general formula (I) in which: - R1 represents a hydrogen atom, an acyl radical or an acyloxy radical; and - R2 represents the side chain of an alpha-amino acid chosen from the group consisting of phenylalanine, glutamic acid, arginine, cysteine, methionine, histidine and tyrosine. The present invention also relates to the use of said cosmetic composition for combating skin redness and / or skin aging; to its use for preventing and / or combating cutaneous vascular hyperproliferation and / or cutaneous inflammation and / or rosacea; and to a method for selecting compounds suitable for preventing and / or combating these pathologies.

The invention discloses an L-cysteine doped ammonium vanadate positive electrode material, a preparation method thereof and application of the L-cysteine doped ammonium vanadate positive electrode material in an aqueous zinc ion battery, and belongs to the technical field of materials. The preparation method comprises the following steps: adding ammonium metavanadate and oxalic acid into deionized water, stirring and mixing, then adding L-cysteine, carrying out hydrothermal reaction on the obtained mixed solution, cooling to room temperature, carrying out centrifugal cleaning, and carrying out vacuum drying to obtain the L-NVO. L-NVO is prepared through a simple one-step hydrothermal method, L-cysteine is doped into the electrode material through further reaction, the introduction of L-cysteine not only enlarges the interlayer spacing of NVO, but also participates in the storage of zinc ions, increases the active sites of the zinc ions, effectively promotes the diffusion of ions and charge transfer, and improves the electrochemical performance of the electrode material. The conductivity of the material is improved, the internal resistance of the material is reduced, and the structural flexibility and stability of the material are maintained, so that the overall performance of the aqueous zinc ion battery is improved.

The present disclosure relates to a method of capping, reducing, and oxidizing cys-mAbs in order to provide homogenous material for subsequent conjugation reactions. The present method demonstrates robust ways to manufacture conjugates of cysteine-engineered antibodies that offer high yield and consistent product quality.

The application discloses a low-foam acid zinc plating additive and a preparation process thereof, and belongs to the technical field of electroplating additives. The additive comprises a low-foam softener and a water-based brightener. The low-foam softener comprises the following raw materials in percentage by mass: polyethylene imine 0.8-1.2%, monododecyl phosphate potassium 0.03-0.06%, water-soluble polyether amine 0.2-0.6%, and the balance is water. The water-based brightener comprises the following raw materials: 5-hydroxymethyl furfural 0.6-1.2%, L-cysteine salt 0.2-0.4%, 4-amino-2(1H) pyrimidinone 0.1-0.15%, sodium salicylate 0.05-0.1%, citric acid 1-1.5%, alpha-ketoglutaric acid 0.3-0.5%, isoascorbic acid 0.2-0.4%, and the balance is water. The application optimizes the components in the additive, prepares a water-based formula, and the components are uniformly dispersed, the foam amount is low, and the plating layer quality is good.

To provide a human interferon-beta mutant having a double mutation and a method for improving the safety of the human interferon-beta mutant.SOLUTION: Provided are a human interferon-beta variant comprising an amino acid sequence in which the 17th amino acid cysteine of human interferon-beta is substituted with serine and the 27th amino acid arginine is substituted with threonine, and a method for improving the stability of a human interferon-beta R27T variant, the method comprising the step of changing the 17th amino acid cysteine of a human interferon-beta R27T variant, in which the 27th amino acid arginine of human interferon-beta is substituted with threonine, to serine.SELECTED DRAWING: Figure 1

The application discloses a capillary open-tube chromatographic column preparation method based on a defect engineering metal organic framework-molecular imprinting composite nanomaterial and application, and particularly relates to the field of chemical synthesis and analysis technology. A GLYMO-IDA-silane solution is used to flush a pretreated capillary, so that a carboxyl-modified capillary is obtained. Then, an N,N-dimethylformamide solution containing zirconyl chloride octahydrate, terephthalic acid, L-proline and hydrochloric acid is injected into the cysteine-modified capillary, and under a heating condition, a metal organic framework UiO-66-L-Pro is in-situ grown on the inner wall of the capillary. D-tryptophan and norepinephrine are dissolved in a neutral buffer solution, and after magnetic stirring for 30 min, ferrous sulfate is added and rapidly injected into the UiO-66-L-Pro-modified capillary, and then both ends are sealed, and reaction is carried out at room temperature for 24 h. An acetic acid / SDS solution mixture is used to flush the capillary for 1.5 h to elute the template molecules, and then nitrogen is used to blow dry, so that an MIP@UiO-66-L-Pro@capillary is prepared.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The application discloses a toughened composite polyurethane impact-resistant material based on dynamic covalent bond energy regulation, and belongs to the technical field of polyurethane materials. A double dynamic covalent network containing DA bonds and disulfide bonds is constructed by reacting polyurethane prepolymer, furfurylamine, cystine diethyl ester and bismaleimide, then nano molybdenum disulfide is modified by silane, then a metal-polyphenol / polymer composite interface layer is constructed on the surface of aramid fiber by tannic acid-iron complex and polyethyleneimine, finally, the components are mixed, impregnated and hot-pressed to obtain the composite polyurethane material. The application effectively dissipates impact energy through the reversible rupture and recombination of dynamic covalent bonds, and enhances the interfacial bonding force between components by using multiple interface modification, so that the material has excellent impact resistance, high strength, high ductility and efficient self-repairing function, solves the problems of easy damage of the interface of traditional composite materials, insufficient impact resistance and irreversible damage, and has wide application prospect in the fields of safety protection and buffer components.

The invention belongs to the technical field of functional food, and particularly relates to a donkey-hide gelatin peptide-iron chelate preparation and a preparation method thereof. The preparation method comprises the following steps: adding colla corii asini powder into water, and melting by heat to obtain a colla corii asini stock solution; adding protease into the colla corii asini stock solution for enzymolysis to obtain colla corii asini enzymatic hydrolysate; bOC-L-cysteine-curcumin, tetrabutylammonium bromide and iodine are added into the colla corii asini enzymatic hydrolysate, a sodium hydroxide solution with the concentration of 0.1 mol / L is adopted for adjusting the pH value, then a reaction is conducted, and a colla corii asini enzymatic hydrolysate retreatment solution is prepared; ascorbic acid is added into the donkey-hide gelatin enzymolysis retreatment liquid, the pH is adjusted with a 10% hydrochloric acid solution, then ferrous chloride is added, centrifugation is carried out after chelation, nanofiltration treatment is carried out, and a nanofiltration concentrated solution is a donkey-hide gelatin peptide-iron chelate; and uniformly stirring and mixing the colla corii asini peptide-iron chelate and genkwanin to obtain the colla corii asini peptide-iron chelate

The invention discloses a spiral chiral gold nanorod as well as a preparation method and application thereof, and the method comprises the following steps: (1) sequentially adding hexadecyl trimethyl ammonium bromide, cysteine and p-aminothiophenol into an anisotropic gold nanorod solution, uniformly mixing, and adsorbing; and (2) silver nitrate, chloroauric acid and ascorbic acid are added for a reaction, centrifugal dispersion is performed after the reaction is finished, and the spiral chiral gold nanorod is obtained. Dispersing the spiral chiral gold nanorod in an aqueous solution, adding phenylalanine enantiomer, dropwise adding on a clean glass slide after incubation is finished, and performing surface enhanced Raman spectroscopy test by using a Raman microscope after air drying. According to the invention, the broadband coverage of chiral response from visible light to near ultraviolet region is realized by accurately regulating and controlling the arrangement of interface molecules and the morphology of the nano structure.

The application belongs to the technical field of material preparation, and relates to a bimodal functional imaging nanomaterial and a preparation method and application thereof. 1‑x Fe x Se2, wherein 0 < x < 1. The preparation method of the bimodal functional imaging nanomaterial comprises the following steps: stirring ferrous sulfate and cysteine in a solvent until uniform, then fully stirring bismuth salt, then adding sodium selenite to perform a heating reaction to obtain a bismuth iron selenium nanocomposite. The bismuth iron selenium nanocomposite provided by the application is uniform in size, regular in morphology, has good water phase dispersibility, and has the performance of simultaneously enhancing MRI imaging and CT imaging.