238results about "Glucose production" patented technology

The invention discloses a method for efficiently preparing furfural and glucose from forest grass biomass. The method comprises the following steps: mixing a forest grass biomass raw material, a molten salt hydrate and an organic solvent, and carrying out a pretreatment reaction to obtain a furfural-containing pretreatment solution and pretreatment residues; mixing the pretreated residues, sulfuric acid and molten salt hydrate, and then performing hydrolysis reaction to obtain hydrolysate containing glucose; the method for preparing furfural and glucose from forest grass biomass provided by the invention is simple, convenient to operate, environment-friendly, short in time, high in yield, economical and efficient, and has a wide industrial application prospect.

The invention relates to a method for converting lignocellulosic biomass by contacting pretreated lignocellulosic biomass with at least one biocatalyst (3) in an aqueous phase in a first reactor (1) containing a reaction medium, the reaction medium comprises the pretreated lignocellulosic biomass (2) and the biocatalyst in an aqueous phase, the process comprises: (a) a first liquefaction step without removing all or part of the reaction medium from the reactor by adding the pretreated lignocellulosic biomass and at least one biocatalyst to the reactor, followed by (b) a second continuous liquefaction step, wherein a portion of the reaction medium is continuously withdrawn from the first reactor, at least one biocatalyst is added, and pretreated lignocellulosic biomass is continuously added.

The invention discloses a parameter differential separation process, and relates to the technical field of separation. The defects in the prior art are overcome. The method specifically comprises the following steps: carrying out a generalized separation process by using a parameter differential moving bed, and operating the generalized separation process to eliminate a displacement zone in chromatographic sequential operation; the method comprises the following steps: contacting a mobile-phase liquid feeding solution with solid-phase resin / adsorbent filler through a novel mass transfer equilibrium contact method, and starting to obtain a characteristic separation curve; the multiple components contained in the mobile phase liquid feed solution are separated by a new mass transfer equilibrium contact process such that at least one component contained in the mobile phase liquid feed solution can be adsorbed onto the resin / adsorbent. The method realizes final separation of a large-scale production separation system, improves the maximum mass transfer efficiency, can also be used for a binary separation system in the separation process of glucose and fructose, reduces the consumption of washing liquid, and reduces the final power consumption.

A device for converting cellulose to sugar has a reaction chamber with a plurality of control components, and a control assembly. The control assembly is operatively connected to the reaction chamber, a drive assembly and control components to transmit and receive interoperability signals. The device has an inlet hopper with a detector, a crusher, an outlet hopper, a sensor assembly, a steam inlet, and a carbon dioxide inlet. The inlet hopper is configured to receive and analyze proportion data of matters in a feedstock and catalyst mixture via the detector. The crusher receives and grinds the mixture from the inlet hopper to induce chemical reaction for producing sugar. The outlet hopper is configured to determine a proportion data of matter in the grinded mixture. The control assembly is configured to determine adjustments need to be performed on the components and drive assembly to optimize the sugar production.

Disclosed in the present invention is a process for preparing a monosaccharide solution by means of acid gradient treatment of a straw lignocellulose. The process comprises the steps of: S1: performing a first acidolysis reaction on a lignocellulose biomass using an acid solution with a concentration of 50 wt% or above to obtain a first acidolysis solution; S2: uniformly mixing the first acidolysis solution with an organic solvent, and performing solid-liquid separation to obtain a glycan-containing residue; and S3: adding water and / or an acid solution to the glycan-containing residue to obtain a mixed solution with an acid concentration of 45 wt% or below, and performing a second acidolysis reaction to obtain a monosaccharide-containing reaction solution. In the present invention, pretreatment is performed at room temperature and atmospheric pressure without the need to provide additional energy, and various agents used in the pretreatment process are all recoverable, which greatly reduces the production cost. In addition, in the present invention, a relatively low liquid-solid ratio is used, monosaccharide can be directly obtained after the pretreatment, and finally a monosaccharide solution with a high and adjustable concentration is obtained, thereby avoiding a large amount of input in enzyme costs.

The present disclosure generally relates to compositions and methods of simultaneously making two unique biointermediates from a single lignocellulosic biomass feedstock, including a hydrophobic biohydrocarbon and a water insoluble hydrophilic cellulosic biopolymer for use in the production of renewable fuels, chemicals, and other carbon neutral materials commonly derived from petroleum and other fossil resources.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention relates to a method for deep processing of corn by using a pressurized soaking method, in particular to a method for preparing dextrin, syrup or sugar, in which the pressurized soaking method is used for replacing a soaking process of a traditional wet corn processing method, so that the corn quickly absorbs water and swells on the premise of not damaging the quality of germs and endosperm and not discharging waste water and waste gas, and the dextrin, the syrup or the sugar is prepared. The process requirements of a wet corn processing method are met, germs and corn husks are further removed, and the obtained germs and corn husks are refined, so that the germs and the corn husks respectively reach commercial selling standards; directly mixing and liquefying the corn slurry without germs and corn husks; the resulting liquefied liquid may be dried to produce dextrins, or further saccharified, refined, concentrated to produce syrup, or optionally further produce sugars.

The invention relates to a method for increasing the yield of glucose biologically converted from corn straws, which comprises the following steps of: carrying out composite pretreatment on the corn straws by using NaOH and urea, and then carrying out step-by-step enzymolysis, namely carrying out enzymolysis on the corn straws by using pleurotus eryngii lysate polysaccharide monooxygenase in the first step, and adding cellulase for further enzymolysis to produce the glucose in the second step, the pleuromutilus rubra lysate polysaccharide monooxygenase is a compound enzyme composed of PdLPMO9A and PdLPMO9B according to a ratio of (1: 2)-(1: 4). According to the method disclosed by the invention, the corn straws are subjected to specific pretreatment, the compound enzyme is formed by adopting the pleurotus eryngii lysate polysaccharide monooxygenase PdLPMO9A and PdLPMO9B according to a specific proportion, and the compound enzyme and the cellulase are used for performing enzymolysis on the corn straws step by step, so that the biological conversion efficiency of the corn straws is effectively improved under the same enzymolysis time, and the yield of glucose is improved and reaches 0.642 g / g of straws.

The application discloses a method for separating non-condensed lignin and saccharide compounds from deconstructed lignocellulose. The lignocellulose is used as raw material, and a high-boiling alcohol aqueous solution such as dihydric alcohol or trihydric alcohol is added into an acid molten salt hydrate as a deconstruction reaction system. The acid molten salt hydrate hydrolyzes hemicellulose and cellulose in the lignocellulose into saccharide compounds, and the high-boiling alcohol in the system acts as a lignin dissolving agent and a structure protecting agent, so that the lignin is dissolved in the high-boiling alcohol. Then, water is added to precipitate the lignin, so that the non-condensed lignin and the saccharide compounds are separated. The biomass deconstruction method can effectively promote the full-component utilization of three components of lignocellulose, and has great industrial application potential.

A system for converting cellulosic feedstock to sugar is disclosed and has a reactor chamber configured receive the cellulosic feedstock, a crusher assembly configured to receive the cellulose feedstock wherein the crusher assembly is configured to grind the mixture under pressure to induce a reaction between the cellulosic feedstock and a natural occurring griding element in the feedstock to produce a grinded mixture and sugar, wherein the crusher assembly comprises rollers.

The present invention relates to the technical field of biotechnology, particularly to an enzyme combination, a genetically modified bacterium, and its use in the production of D-allulose. The present invention relates to Bacillus subtilis co-expressing glucose isomerase and D-allulose 3-epimerase from a specific source. The resulting modified bacterium is fermented to obtain a crude enzyme solution, which is then isomerized using a high concentration of glucose as a substrate. After separation, purification, and concentration, fructoglucose syrup and D-allulose syrup are obtained. The enzyme combination provided by the present invention has a high catalytic rate relative to the substrate, significantly improving the conversion rate of D-allulose. At the same time, the present invention reduces the production costs of D-allulose by using inexpensive glucose as a raw material, and simultaneously produces fructoglucose syrup while producing D-allulose, thereby saving on the input of the fructoglucose syrup production line and significantly improving economic benefits.

A method for treating grain having starch and non-starch carbohydrates, wherein the starch is present in an amount of at least 10% by weight based on the dry weight of the grain. Contacting the grains with a solution containing at least one alpha-hydroxysulfonic acid; and reacting under acid hydrolysis conditions to produce a product suitable for the production of chemicals and / or fuels.

A low-cost process is provided to render lignocellulosic biomass accessible to cellulase enzymes, to produce fermentable sugars. Some variations provide a process to produce ethanol from lignocellulosic biomass (such as sugarcane bagasse or corn stover), comprising introducing a lignocellulosic biomass feedstock to a single-stage digestor; exposing the feedstock to a reaction solution comprising steam or liquid hot water within the digestor, to solubilize the hemicellulose in a liquid phase and to provide a cellulose-rich solid phase; refining the cellulose-rich solid phase, together with the liquid phase, in a mechanical refiner, thereby providing a mixture of refined cellulose-rich solids and the liquid phase; enzymatically hydrolyzing the mixture in a hydrolysis reactor with cellulase enzymes, to generate fermentable sugars; and fermenting the fermentable sugars to produce ethanol. Many alternative process configurations are described. The disclosed processes may be employed for other fermentation products.

The invention relates to enzyme compositions comprising a glucoamylase, an alpha-amylase, and optionally a cellulolytic composition and / or a protease. The invention also relates to the use thereof in processes of producing sugars and / or fermentation products from starch-containing material by saccharifying and / or fermenting starch-containing material at a temperature below the initial gelatinization temperature.

The present invention discloses a method for preparing fuel ethanol from straw biomass and the fuel ethanol, belonging to the technical field of fuel ethanol production. It includes: dissolving and hydrolyzing straw and concentrated sulfuric acid to obtain a saccharified solution, separating out a sugar-rich solution in a simulated moving bed chromatography system, and then mixing it with Saccharomyces cerevisiae SEB3 for fermentation, and generating fuel ethanol through distillation. The method for preparing fuel ethanol from straw biomass provided by the present invention can obtain fermentable sugars under normal pressure and low temperature conditions by using concentrated acid hydrolysis, and has the remarkable advantages of low energy consumption, low toxic by-products, and no need to input cellulase. And Saccharomyces cerevisiae SEB3 is used for fermentation. This strain has the ability of co-fermenting all sugars, can realize the fermentation of all sugars, synchronously convert all sugars into ethanol, the sugar consumption efficiency reaches more than 90%, and the ethanol recovery rate reaches more than 80%, and it is applicable to industrial production.

The application discloses a preparation method of a mesoporous silicon-based composite material with phosphotungstic acid and titanium oxide simultaneously supported, and belongs to the technical field of catalytic material preparation. The method aims to solve the problems of easy aggregation of active components, easy damage of channel order and complex preparation process in the prior art. The method is characterized by the following steps: through a one-step hydrothermal synthesis strategy, a triblock copolymer is used as a structure directing agent, an alcohol solution of a titanium source precursor, a silicon source precursor and a Keggin type phosphotungstic acid is simultaneously added in an acid system, and then the mesoporous silicon-based composite material is prepared through hydrothermal crystallization, washing, drying and calcination. The method is simple in process and controllable in operation, and the obtained material has the following advantages: two-dimensional hexagonal ordered mesoporous structure, large specific surface area, high dispersion of titanium and phosphotungstic acid, intact Keggin structure, good double-acid-site synergistic catalytic effect, and suitability for acid catalytic reactions, especially in the field of biomass conversion.

A method for treating grains having starch and non-starch carbohydrates, wherein the starch is present in an amount of at least 10 wt.% based on the dry weight of the grain. The grain is contacted with a solution containing at least one α-hydroxysulfonic acid; and to react under acid hydrolysis conditions to produce a product that is suitable for producing chemicals and / or fuel.

Described is a process for releasing soluble starch hydrolysates or hydrolysed starch syrup from substantially intact corn kernels during high temperature steeping at a temperature at or above the gelatinization temperature of starch in the corn kernels, and the recovery of protein and oil-enhanced, carbohydrate-depleted residuals that are ideally suited for use as high quality animal feed or other food-grade products.

The method that separates a fermentation inhibitor and sugar from a sugar solution that contains a fermentation inhibitor and recovers the sugar is a method that includes using water as an eluent to separate the fermentation inhibitor and the sugar based on the difference in retention time after having brought the sugar solution that contains a fermentation inhibitor into contact with a basic anion exchange resin packed in a column, and separately recovering a fraction that contains the fermentation inhibitor and a fraction that contains the sugar, in which the basic anion exchange resin is pretreated by a solution that contains a fermentation inhibitor.

The invention relates to a cooling crystallizer (2.0) for saccharose magma in a vertically oriented container (2.1) which has an upper inlet (2.2) for supplying magma and a lower outlet (2.3) for discharging magma, comprising multiple cooling blocks (5.0) which are mutually spaced in a vertical direction. A heat carrier fluid flows through the cooling blocks (5.0), and the cooling blocks are coupled to a heat exchanger in order to dissipate heat from the magma, wherein multiple cooling blocks (5.0) are combined to form a cooling packet (5.1; 5.2), and the cooling packets (5.1; 5.2) are designed as separate cooling circuits with separate heat exchangers (2.1.2; 2.2.2).

The present application relates to starch production technical field, especially disclose a kind of green preparation method for modified starch for replacing hydroxypropyl distarch phosphate.The green preparation method, cassava starch is used as raw material, specifically includes the following steps: (1) cassava starch is mixed with water uniformly, and starch suspension is prepared;(2) adding alpha-amylase to starch suspension for glycosidic bond enzymolysis, then passivate enzyme activity;(3) the enzymatic hydrolysate is used to hexose oxidase and oligomeric glucan is subjected to lactonization reaction, then passivate enzyme activity;(4) the final enzymatic hydrolysate is centrifuged, washed with water, the precipitate is freeze-dried, and enzyme modified cassava starch is obtained.The paste viscosity, freeze-thaw stability, shear resistance, anti-aging and other chemical properties of the enzyme modified cassava starch prepared by the present application are close to or better than hydroxypropyl distarch phosphate, and the preparation process is simple, short cycle, green and safe, low in production cost, simple in equipment, suitable for industrial production, and safe and pollution-free during production.

The present invention relates to a process for producing raw juice (14) for making sugar, wherein sugar-beet cossettes (11) are fed to an extraction device (20), for example an extraction tower or a diffusion trough, and sugar-beet-cossette remains (13) and raw juice (14) are drawn off from the extraction device (20), wherein a first near-infrared spectroscopy device (21) is used to record first measurement data concerning the sugar-beet cossettes (11) which are fed to the extraction device (20) and / or a second near-infrared spectroscopy device (22) is used to record second measurement data concerning the sugar-beet-cossette remains (13) which are drawn off from the extraction device (20) and / or a third near-infrared spectroscopy device (23) is used to record third measurement data concerning the raw juice (14) which is drawn off from the extraction device (20). The invention also relates to a sugar-production installation and to a process for making sugar, wherein raw juice (14) is produced and sugar is made from the raw juice (14) in subsequent process steps.

The invention relates to a method for preparing a fermentation substrate, said method comprising the steps of: providing at least one leguminous plant seed comprising starch and proteins; micronizing the seed so as to obtain a micronized fraction; purifying the micronized fraction so as to collect a starch-enriched and protein-depleted fraction; mixing the starch-enriched and protein-depleted fraction with a liquid so as to form a starch fluid; and hydrolysing the starch by mixing water vapour with the starch fluid so as to obtain a hydrolysed starch fluid. The invention also relates to a fermentation substrate, to a fermentation method, and to a fermentation product.

The invention belongs to the technical field of starch sugar processing, and particularly relates to a preparation method and application of acid-process starch syrup. The preparation method of the acid-process starch syrup comprises the following steps: enabling pure water to pass through a strong acid cation exchange resin column to obtain acidic pure water; then mixing with starch, and performing size mixing to obtain a material A; carrying out heating, pressure maintaining, heat preservation and cooling on the material A to obtain a material B, adjusting the pH of the material B to be acidic, filtering, and taking filtrate to obtain a material C; deacidifying through a weak base anion exchange resin column to obtain a material D; then adjusting the pH to be acidic to obtain a material E; the material E sequentially passes through a sodium type strong acid cation exchange resin column and a chlorine type strong base ion exchange resin column to obtain a material F; and refining through an activated carbon column to obtain a material G, and concentrating to obtain the product. According to the method, the ash content of the acid-process starch hydrolysis saccharification liquid can be reduced, and the excessive sugar multiple of the ion exchange resin column is improved, so that the consumption of an ion exchange resin regenerant is reduced, and the acid-process starch syrup which is relatively low in ash content and not easy to discolor during storage is obtained.

The present invention relates to a process for converting lignocellulosic biomass by contacting, in aqueous phase, pretreated lignocellulosic biomass with at least one biocatalyst (3) in a first reactor (1) containing a reaction medium comprising said pretreated lignocellulosic biomass (2) in aqueous phase and said biocatalyst, said process comprising: (a) a first liquefaction step by adding said pretreated lignocellulosic biomass and at least one biocatalyst to said reactor without withdrawing all or part of said reaction medium from said reactor, and (b) a second continuous liquefaction step, with continuous withdrawal from said first reactor of a portion of said reaction medium, addition of at least one biocatalyst, and continuous addition of pretreated lignocellulosic biomass. Figure for the abstract: Fig. 2

The invention discloses a method for efficiently saccharifying brewing sorghum straws, which comprises the following steps: pretreating the brewing sorghum straws by adopting steam explosion under the conditions that the explosion pressure is 0.9-1.5 MPa, the pressure maintaining time is 4-8 minutes, the particle size of a material is 3-9 cm and the water content of the material is 30-70%; carrying out enzymolysis saccharification on the brewing sorghum straws subjected to steam explosion treatment, wherein the initial solid content is 10-20%, the enzyme addition amount is 20-50 FPU / g, the enzymolysis temperature is 45-55 DEG C, and the material particle size is 10-30 meshes; in the enzymolysis saccharification process, materials are supplemented in stages to regulate and control the solid content, the materials are supplemented at least once, and the final solid content is 35-40%. According to the method disclosed by the invention, the biomass conversion efficiency is remarkably improved through system optimization pretreatment and an enzymolysis process, and the solid content is improved and the fermentable sugar concentration is remarkably improved in combination with stepped material supplementation.

Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with low mechanical energy input. In some variations, the process includes fractionating biomass with sulfur dioxide or a sulfite compound and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and / or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites.

The present invention provides an improved method for processing crop grains to provide high-quality starch products suitable for converting starch into monosaccharides and oligosaccharides, ethanol, and sweeteners. The present invention also provides polypeptides having GH10 xylanase activity, polypeptides having GH62 arabinofuranosidase activity, and their uses.

Provided herein are methods and compositions for reducing the viscosity of syrup produced as a byproduct of fermentation processes.