168results about "Glucose production" patented technology

The present disclosure generally relates to compositions and methods of simultaneously making two unique biointermediates from a single lignocellulosic biomass feedstock, including a hydrophobic biohydrocarbon and a water insoluble hydrophilic cellulosic biopolymer for use in the production of renewable fuels, chemicals, and other carbon neutral materials commonly derived from petroleum and other fossil resources.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention relates to a method for deep processing of corn by using a pressurized soaking method, in particular to a method for preparing dextrin, syrup or sugar, in which the pressurized soaking method is used for replacing a soaking process of a traditional wet corn processing method, so that the corn quickly absorbs water and swells on the premise of not damaging the quality of germs and endosperm and not discharging waste water and waste gas, and the dextrin, the syrup or the sugar is prepared. The process requirements of a wet corn processing method are met, germs and corn husks are further removed, and the obtained germs and corn husks are refined, so that the germs and the corn husks respectively reach commercial selling standards; directly mixing and liquefying the corn slurry without germs and corn husks; the resulting liquefied liquid may be dried to produce dextrins, or further saccharified, refined, concentrated to produce syrup, or optionally further produce sugars.

The invention relates to a method for increasing the yield of glucose biologically converted from corn straws, which comprises the following steps of: carrying out composite pretreatment on the corn straws by using NaOH and urea, and then carrying out step-by-step enzymolysis, namely carrying out enzymolysis on the corn straws by using pleurotus eryngii lysate polysaccharide monooxygenase in the first step, and adding cellulase for further enzymolysis to produce the glucose in the second step, the pleuromutilus rubra lysate polysaccharide monooxygenase is a compound enzyme composed of PdLPMO9A and PdLPMO9B according to a ratio of (1: 2)-(1: 4). According to the method disclosed by the invention, the corn straws are subjected to specific pretreatment, the compound enzyme is formed by adopting the pleurotus eryngii lysate polysaccharide monooxygenase PdLPMO9A and PdLPMO9B according to a specific proportion, and the compound enzyme and the cellulase are used for performing enzymolysis on the corn straws step by step, so that the biological conversion efficiency of the corn straws is effectively improved under the same enzymolysis time, and the yield of glucose is improved and reaches 0.642 g / g of straws.

The application discloses a method for separating non-condensed lignin and saccharide compounds from deconstructed lignocellulose. The lignocellulose is used as raw material, and a high-boiling alcohol aqueous solution such as dihydric alcohol or trihydric alcohol is added into an acid molten salt hydrate as a deconstruction reaction system. The acid molten salt hydrate hydrolyzes hemicellulose and cellulose in the lignocellulose into saccharide compounds, and the high-boiling alcohol in the system acts as a lignin dissolving agent and a structure protecting agent, so that the lignin is dissolved in the high-boiling alcohol. Then, water is added to precipitate the lignin, so that the non-condensed lignin and the saccharide compounds are separated. The biomass deconstruction method can effectively promote the full-component utilization of three components of lignocellulose, and has great industrial application potential.

A system for converting cellulosic feedstock to sugar is disclosed and has a reactor chamber configured receive the cellulosic feedstock, a crusher assembly configured to receive the cellulose feedstock wherein the crusher assembly is configured to grind the mixture under pressure to induce a reaction between the cellulosic feedstock and a natural occurring griding element in the feedstock to produce a grinded mixture and sugar, wherein the crusher assembly comprises rollers.

The present invention relates to the technical field of biotechnology, particularly to an enzyme combination, a genetically modified bacterium, and its use in the production of D-allulose. The present invention relates to Bacillus subtilis co-expressing glucose isomerase and D-allulose 3-epimerase from a specific source. The resulting modified bacterium is fermented to obtain a crude enzyme solution, which is then isomerized using a high concentration of glucose as a substrate. After separation, purification, and concentration, fructoglucose syrup and D-allulose syrup are obtained. The enzyme combination provided by the present invention has a high catalytic rate relative to the substrate, significantly improving the conversion rate of D-allulose. At the same time, the present invention reduces the production costs of D-allulose by using inexpensive glucose as a raw material, and simultaneously produces fructoglucose syrup while producing D-allulose, thereby saving on the input of the fructoglucose syrup production line and significantly improving economic benefits.

A method for treating grain having starch and non-starch carbohydrates, wherein the starch is present in an amount of at least 10% by weight based on the dry weight of the grain. Contacting the grains with a solution containing at least one alpha-hydroxysulfonic acid; and reacting under acid hydrolysis conditions to produce a product suitable for the production of chemicals and / or fuels.

A low-cost process is provided to render lignocellulosic biomass accessible to cellulase enzymes, to produce fermentable sugars. Some variations provide a process to produce ethanol from lignocellulosic biomass (such as sugarcane bagasse or corn stover), comprising introducing a lignocellulosic biomass feedstock to a single-stage digestor; exposing the feedstock to a reaction solution comprising steam or liquid hot water within the digestor, to solubilize the hemicellulose in a liquid phase and to provide a cellulose-rich solid phase; refining the cellulose-rich solid phase, together with the liquid phase, in a mechanical refiner, thereby providing a mixture of refined cellulose-rich solids and the liquid phase; enzymatically hydrolyzing the mixture in a hydrolysis reactor with cellulase enzymes, to generate fermentable sugars; and fermenting the fermentable sugars to produce ethanol. Many alternative process configurations are described. The disclosed processes may be employed for other fermentation products.

The invention relates to enzyme compositions comprising a glucoamylase, an alpha-amylase, and optionally a cellulolytic composition and / or a protease. The invention also relates to the use thereof in processes of producing sugars and / or fermentation products from starch-containing material by saccharifying and / or fermenting starch-containing material at a temperature below the initial gelatinization temperature.

The application discloses a preparation method of a mesoporous silicon-based composite material with phosphotungstic acid and titanium oxide simultaneously supported, and belongs to the technical field of catalytic material preparation. The method aims to solve the problems of easy aggregation of active components, easy damage of channel order and complex preparation process in the prior art. The method is characterized by the following steps: through a one-step hydrothermal synthesis strategy, a triblock copolymer is used as a structure directing agent, an alcohol solution of a titanium source precursor, a silicon source precursor and a Keggin type phosphotungstic acid is simultaneously added in an acid system, and then the mesoporous silicon-based composite material is prepared through hydrothermal crystallization, washing, drying and calcination. The method is simple in process and controllable in operation, and the obtained material has the following advantages: two-dimensional hexagonal ordered mesoporous structure, large specific surface area, high dispersion of titanium and phosphotungstic acid, intact Keggin structure, good double-acid-site synergistic catalytic effect, and suitability for acid catalytic reactions, especially in the field of biomass conversion.

A method for treating grains having starch and non-starch carbohydrates, wherein the starch is present in an amount of at least 10 wt.% based on the dry weight of the grain. The grain is contacted with a solution containing at least one α-hydroxysulfonic acid; and to react under acid hydrolysis conditions to produce a product that is suitable for producing chemicals and / or fuel.

Described is a process for releasing soluble starch hydrolysates or hydrolysed starch syrup from substantially intact corn kernels during high temperature steeping at a temperature at or above the gelatinization temperature of starch in the corn kernels, and the recovery of protein and oil-enhanced, carbohydrate-depleted residuals that are ideally suited for use as high quality animal feed or other food-grade products.

The present application relates to starch production technical field, especially disclose a kind of green preparation method for modified starch for replacing hydroxypropyl distarch phosphate.The green preparation method, cassava starch is used as raw material, specifically includes the following steps: (1) cassava starch is mixed with water uniformly, and starch suspension is prepared;(2) adding alpha-amylase to starch suspension for glycosidic bond enzymolysis, then passivate enzyme activity;(3) the enzymatic hydrolysate is used to hexose oxidase and oligomeric glucan is subjected to lactonization reaction, then passivate enzyme activity;(4) the final enzymatic hydrolysate is centrifuged, washed with water, the precipitate is freeze-dried, and enzyme modified cassava starch is obtained.The paste viscosity, freeze-thaw stability, shear resistance, anti-aging and other chemical properties of the enzyme modified cassava starch prepared by the present application are close to or better than hydroxypropyl distarch phosphate, and the preparation process is simple, short cycle, green and safe, low in production cost, simple in equipment, suitable for industrial production, and safe and pollution-free during production.

The present invention relates to a process for producing raw juice (14) for making sugar, wherein sugar-beet cossettes (11) are fed to an extraction device (20), for example an extraction tower or a diffusion trough, and sugar-beet-cossette remains (13) and raw juice (14) are drawn off from the extraction device (20), wherein a first near-infrared spectroscopy device (21) is used to record first measurement data concerning the sugar-beet cossettes (11) which are fed to the extraction device (20) and / or a second near-infrared spectroscopy device (22) is used to record second measurement data concerning the sugar-beet-cossette remains (13) which are drawn off from the extraction device (20) and / or a third near-infrared spectroscopy device (23) is used to record third measurement data concerning the raw juice (14) which is drawn off from the extraction device (20). The invention also relates to a sugar-production installation and to a process for making sugar, wherein raw juice (14) is produced and sugar is made from the raw juice (14) in subsequent process steps.

The invention relates to a method for preparing a fermentation substrate, said method comprising the steps of: providing at least one leguminous plant seed comprising starch and proteins; micronizing the seed so as to obtain a micronized fraction; purifying the micronized fraction so as to collect a starch-enriched and protein-depleted fraction; mixing the starch-enriched and protein-depleted fraction with a liquid so as to form a starch fluid; and hydrolysing the starch by mixing water vapour with the starch fluid so as to obtain a hydrolysed starch fluid. The invention also relates to a fermentation substrate, to a fermentation method, and to a fermentation product.

The invention belongs to the technical field of starch sugar processing, and particularly relates to a preparation method and application of acid-process starch syrup. The preparation method of the acid-process starch syrup comprises the following steps: enabling pure water to pass through a strong acid cation exchange resin column to obtain acidic pure water; then mixing with starch, and performing size mixing to obtain a material A; carrying out heating, pressure maintaining, heat preservation and cooling on the material A to obtain a material B, adjusting the pH of the material B to be acidic, filtering, and taking filtrate to obtain a material C; deacidifying through a weak base anion exchange resin column to obtain a material D; then adjusting the pH to be acidic to obtain a material E; the material E sequentially passes through a sodium type strong acid cation exchange resin column and a chlorine type strong base ion exchange resin column to obtain a material F; and refining through an activated carbon column to obtain a material G, and concentrating to obtain the product. According to the method, the ash content of the acid-process starch hydrolysis saccharification liquid can be reduced, and the excessive sugar multiple of the ion exchange resin column is improved, so that the consumption of an ion exchange resin regenerant is reduced, and the acid-process starch syrup which is relatively low in ash content and not easy to discolor during storage is obtained.

The present invention relates to a process for converting lignocellulosic biomass by contacting, in aqueous phase, pretreated lignocellulosic biomass with at least one biocatalyst (3) in a first reactor (1) containing a reaction medium comprising said pretreated lignocellulosic biomass (2) in aqueous phase and said biocatalyst, said process comprising: (a) a first liquefaction step by adding said pretreated lignocellulosic biomass and at least one biocatalyst to said reactor without withdrawing all or part of said reaction medium from said reactor, and (b) a second continuous liquefaction step, with continuous withdrawal from said first reactor of a portion of said reaction medium, addition of at least one biocatalyst, and continuous addition of pretreated lignocellulosic biomass. Figure for the abstract: Fig. 2

The invention discloses a method for efficiently saccharifying brewing sorghum straws, which comprises the following steps: pretreating the brewing sorghum straws by adopting steam explosion under the conditions that the explosion pressure is 0.9-1.5 MPa, the pressure maintaining time is 4-8 minutes, the particle size of a material is 3-9 cm and the water content of the material is 30-70%; carrying out enzymolysis saccharification on the brewing sorghum straws subjected to steam explosion treatment, wherein the initial solid content is 10-20%, the enzyme addition amount is 20-50 FPU / g, the enzymolysis temperature is 45-55 DEG C, and the material particle size is 10-30 meshes; in the enzymolysis saccharification process, materials are supplemented in stages to regulate and control the solid content, the materials are supplemented at least once, and the final solid content is 35-40%. According to the method disclosed by the invention, the biomass conversion efficiency is remarkably improved through system optimization pretreatment and an enzymolysis process, and the solid content is improved and the fermentable sugar concentration is remarkably improved in combination with stepped material supplementation.

Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with low mechanical energy input. In some variations, the process includes fractionating biomass with sulfur dioxide or a sulfite compound and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and / or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites.

The invention relates to a method for treating lignocellulosic biomass, comprising: b) a step of acidic or neutral pretreatment of the biomass to produce an acidic or neutral pretreated biomass, which is carried out alternately with b1), b1) a step of alkaline pre-treatment of the biomass in advance under acidic, neutral or alkaline conditions to produce an alkaline pre-treated biomass, followed by c) a step of enzymatic hydrolysis of the acidic pre-treated biomass and / or the alkaline pre-treated biomass to obtain a hydrolyzed biomass, d) a step of solid / liquid separation of the hydrolyzed biomass in sugar form obtained at the end of step c) or a step of solid / liquid separation of the hydrolyzed biomass in sugar form obtained at the end of step c) and then treated in a step following enzymatic hydrolysis of step c), to obtain separated juice and unconverted solid residues, e) a step of recycling said solid residue to the alkaline pretreatment step b ').

The present application belongs to the technical field of glucose preparation, and particularly relates to a process for producing glucose by using corn dry method. The process for producing glucose by using corn dry method provided by the present application comprises the steps of corn kernel pretreatment, freezing treatment, high-pressure steam explosion treatment, liquefaction, saccharification, refining and the like. The process for producing glucose by using corn dry method provided by the present application combines freezing and thawing, high-pressure steam explosion and multi-stage enzymolysis and the like process conditions for regulation and control, effectively improves the liquefaction efficiency, enhances the saccharification effect and speeds up the saccharification reaction speed, so that high-purity glucose with stable quality is quickly obtained, which meets the current standard and specification requirements. Moreover, the process can shorten the time required for liquefaction and saccharification, effectively reduce energy consumption and lower the overall production cost, and a more efficient conversion process ensures that more starch is converted into glucose, improves the raw material use efficiency, and maximizes the reduction of resource waste.

The utility model relates to the technical field of glucose mother liquor treatment equipment, in particular to a glucose mother liquor treatment system which comprises a first nanofiltration unit, a hydrolysis unit, a continuous chromatographic separation unit and a second acid-resistant nanofiltration unit which are sequentially connected according to treatment procedures. The first nanofiltration unit is used for carrying out primary nanofiltration treatment on the glucose mother liquor and sending a generated concentrated solution I into the hydrolysis unit, the hydrolysis unit is used for carrying out acid hydrolysis on the concentrated solution I and conveying a generated hydrolysate to the continuous chromatographic separation unit, and the continuous chromatographic separation unit is used for carrying out deacidification treatment on the hydrolysate; acid liquor generated in the deacidification treatment process of the continuous chromatographic separation system is fed into a second acid-resistant nanofiltration unit to be subjected to secondary nanofiltration treatment. The treatment system is reasonable in process, solves the problems of large acid and alkali consumption and high water consumption in the existing glucose mother liquor treatment process, and can greatly improve the recovery rate of glucose.

The present invention belongs to the field of biomass conversion, and in particular to a process for preparing sugar by enzymolysis of biomass, wherein the biomass is pretreated; an enzymolysis aid is prepared, and in the presence of the enzymolysis aid, the pretreated biomass is subjected to enzymatic hydrolysis; after the hydrolysis is completed, the supernatant is taken, the temperature is increased, the enzymolysis aid is precipitated, and an enzymolysis solution is obtained; the enzymolysis aid is cleaned and recycled; the enzymolysis solution is purified and the sugar therein is extracted. The method of the present invention has a wide scope of application, high enzymolysis efficiency, low enzyme input cost, and the enzymolysis aid is easily separated, does not pollute downstream production, and is easily recyclable, with low production cost.

According to the present invention, it is possible to efficiently produce proteins by culturing a filamentous fungus belonging to the genus Trichoderma using a medium containing a corn hull pulverization product having a relative particle mass peak in a particle size range of 70-150 µm in a volume-based particle size distribution obtained by a measuring method based on a laser diffraction and scattering method.

The application discloses a preparation method and application of a supported nickel-based catalyst, and relates to the technical field of catalysts, in particular to a preparation method of a supported nickel-based catalyst.The method comprises the following steps: mixing and stirring nickel nitrate hexahydrate and lanthanide nitrate, adding a biomass template agent in the stirring process, and obtaining a wet gel through water bath heating, wherein the molar ratio of C6 of the biomass template agent to the total mass of the nickel nitrate hexahydrate and the lanthanide nitrate is 1.5:1-5:1; drying, pre-calcining, calcining, activating and passivating the wet gel to obtain the supported nickel-based catalyst, and the pre-calcining temperature is 300-500 DEG C.The preparation method is simple, and the high specific surface area can effectively strengthen hydrogen dissociation and adsorption and activation of substrate molecules such as glucose, so that efficient conversion of glucose and maleic anhydride is realized.

This invention discloses a mesoporous silica-based composite material with phosphotungstic acid simultaneously immobilized with titanium oxide and its cellulose hydrolysis method, belonging to the field of biomass catalytic conversion technology. This invention aims to solve the problems of easy desorption of active components, low catalytic efficiency, and poor cycle stability in existing solid acid catalysts during cellulose hydrolysis. This method uses an ionic liquid as the reaction medium and a titanium-silicon-supported phosphotungstic acid composite material with a two-dimensional hexagonal ordered mesoporous structure prepared by a one-step hydrothermal synthesis as the solid acid catalyst, catalyzing the hydrolysis of cellulose to reduce sugars in the presence of water. This invention achieves highly efficient cellulose conversion through the synergistic catalytic effect of the two acid sites combined with the good solubility of cellulose in the ionic liquid. The catalyst exhibits excellent recyclability, the process conditions are well-defined, it meets the requirements of green chemistry, and it is applicable to the field of biomass resource utilization.

A method for hydrolyzing cellulose into sugar to produce spherical capacitive carbon for the deep utilization of biomass and carbon materials. The present disclosure includes the following steps of: (1) crude cellulose pretreatment; (2) alkaline hydrolysis of cellulose; (3) separation of the cellulose from a hydrolyzed sugar liquor; (4) drying of an alkali-containing hydrolyzed sugar; (5) sintering of spherical capacitive carbon; (6) capacitive carbon post-processing; and (7) alkali recycling. In the method, biomass is used as a raw material, high-purity cellulose and hydrolyzed sugar are obtained through deep hydrolysis, the spherical capacitive carbon is sintered with the hydrolyzed sugar instead of sucrose and starch, and alkali is recycled. Pollution and waste are not generated, and more than 80% of the alkali can be recycled.

The invention relates to a process for treating lignocellulosic biomass, employing at least one reactor (9; 14) for treating said biomass, said reactor (9; 14) being equipped with a feeding device (6; 11) having a biomass inlet and a biomass outlet, said biomass outlet being in fluidic connection with an inlet of the reactor, such that: - a residue (8; 13) is extracted from the biomass as it passes through the feeding device (6; 11) to the reactor (9; 14) via an extraction outlet provided in said device, said residue being a mixture of solid and liquid; - said residue is separated into a solid residue (32; 42) and a liquid residue (31; 41); - at least a portion of the solid residue (32; 42) is reintroduced into the feeding device, or into one of said feeding devices. Figure for the abstract: Fig. 2

The invention relates to a preparation method of a saccharide isomeride with strong moisturizing and relieving effects as well as a product and application of the saccharide isomeride. The preparation method comprises the following steps: mixing corn starch with water to prepare starch milk, and performing acidolysis, neutralization, composite enzymolysis, saccharification and cooling crystallization to prepare D-glucose crystals; mixing the D-glucose crystal with water and glucose isomerase for reaction, and performing enzyme deactivation and concentration to obtain a saccharide isomeride; wherein the enzyme in the composite enzymatic hydrolysis comprises a combination of alpha-amylase, pullulanase and beta-amylase. The specific enzyme combination is adopted to decompose and extract the saccharide isomeride component in the corn starch, a potential synergistic relationship exists among the three enzymes, the extraction effect can be remarkably improved after the three enzymes are combined, and the prepared saccharide isomeride product has excellent moisturizing and soothing effects.