345 results about "Carbohydrase" patented technology

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention belongs to the technical field of microorganisms, and discloses a drying protective agent for a brewed food fungicide, application of the drying protective agent and a preparation method of the brewed food fungicide. The brewing food fungicide drying protective agent provided by the invention comprises sterilized sorghum hydrolysate, the sorghum hydrolysate is hydrolysate obtained after a sorghum suspension is subjected to enzymolysis with alpha-amylase and saccharifying enzyme. The protective agent provided by the invention contains rich polysaccharides, monosaccharides, proteins, vitamins, minerals, organic acids and antioxidant substances, can provide comprehensive nutritional support for strains, has multiple protection mechanisms, can reduce damage in the drying and storage processes, improves the survival rate and activity of the strains in the drying and storage processes, and improves the survival rate of the strains in the drying and storage processes. And rapid recovery and metabolism start of the strain are promoted. And meanwhile, the single sorghum hydrolysate is used as a protective agent, so that the purity of the brewing process and the flavor stability of the product can be ensured. Therefore, the drying protective agent provided by the invention has obvious advantages and wide application prospects.

The invention belongs to the technical field of microbial fermentation, and discloses saccharomycopsis fibuligera and application thereof in rice wine. The saccharomycopsis fibuligera strain disclosed by the invention is Saccharomycopsis fibuligera ZB440, and the preservation number of the saccharomycopsis fibuligera strain is GDMCC No: 64395. The saccharomycopsis fibuligera has the characteristics of high temperature resistance, acid resistance, high alcohol resistance, high saccharifying enzyme activity, high esterifying enzyme activity and high alcohol yield. The method can be applied to rice wine fermentation, and the flavor substances of esters, alcohols, aldehydes and ketones can be obviously improved.

The invention relates to the technical field of microorganisms, in particular to an Aspergillus oryzae ZA307 strain and application of the Aspergillus oryzae ZA307 strain. The aspergillus oryzae ZA307 high-yield protease and aminopeptidase provided by the invention cooperate with aspergillus oryzae Huniang 3.042 for starter propagation, so that the activity of the protease, aminopeptidase, saccharifying enzyme and amylase of a starter material can be remarkably improved, and furthermore, the content of ammonia nitrogen and total nitrogen and the variety and content of volatile flavor substances in a product are remarkably improved. The soy sauce or soybean paste prepared and fermented by double-strain starter propagation is obviously improved in the aspects of sauce fragrance, ester fragrance, delicate flavor and harmonious taste, and the quality of the product is comprehensively improved.

The invention discloses a high-stability oat milk beverage and a preparation method thereof, and belongs to the technical field of grain deep processing. The preparation method of the oat milk beverage comprises the following steps: cleaning and drying oat to obtain dry oat, adding water, grinding into thick liquid, and boiling and gelatinizing to obtain gelatinized liquid; adding alpha-amylase, debranching enzyme, saccharifying enzyme and neutral protease into the gelatinized liquid, stirring, performing enzymolysis, performing enzyme deactivation, sieving, and collecting slurry; and finally homogenizing to obtain the oat milk. According to the preparation method disclosed by the invention, the stability of the oat milk is improved by optimizing process conditions and enzymes used for enzymolysis in the preparation of the oat milk, the preparation of the functional oat milk is realized by further adding functional components under the condition that the stability of the oat milk is not influenced, and the prepared oat milk can realize various effects of reducing blood sugar, resisting fatigue and the like.

The method for preparing D-mannose through catalysis can effectively reduce the amount of by-products glucose and fructose, and greatly improves the conversion rate of D-mannose. Specifically, engineering bacteria for expressing isoamylase genes and engineering bacteria for expressing alpha-glucan phosphorylase genes, glucophosphate mutase genes, difunctional enzyme glucophosphate isomerase / mannose 6-phosphate isomerase genes and mannose 6-phosphate phosphatase genes are utilized, starch or starch derivatives are used as substrates, and the starch or starch derivatives are used as substrates. A phosphate buffer solution and Mg < 2 + > are added, a preliminary catalysis system is constructed, after the reaction is completed, an incompletely-reacted substrate and maltodisaccharide exist in the reaction system, the incompletely-reacted substrate and maltodisaccharide can be hydrolyzed into glucose by glucoamylase, and the polyphosphoglucokinase can be used for catalyzing the reaction of the polyphosphoglucokinase under the assistance of sodium hexametaphosphate. All glucose is converted into phosphorylated glucose, then D-mannose is generated, and by-products in a system are removed while the yield is increased.

The invention discloses a preparation method of V-shaped adsorptive starch, and belongs to the technical field of starch modification. The preparation method comprises the following steps: firstly, carrying out high-temperature alcohol-water heat treatment to obtain V-type starch with a V-type single-spiral structure, expanded and loose particles, a large amount of exposed hydroxyl groups and relatively high reaction activity; pullulanase which has a poor enzymolysis effect on original starch and is not applied to enzymolysis of the V-type starch is subsequently adopted for enzymolysis, due to the fact that pullulanase has different enzymolysis characteristics from common saccharifying enzyme and alpha-amylase, short-chain dextrin formed during enzymolysis can be combined with ethyl alcohol to form a V-type compound with an adsorption effect, the V-type compound can be precipitated by the ethyl alcohol, and then the V-type compound can be separated and purified. The utilization rate of enzymolysis byproducts is improved, so that the yield of final products can be improved.

The invention discloses a preparation method of a whole-bean rumex hanus nutrient plant enzyme, which comprises the following steps: S1, taking soybeans, and preparing whole-bean minced soybean; taking rumex hanus to prepare rumex hanus whole pulp; taking grains, and preparing whole grain pulp paste; taking fruits and vegetables, and preparing fruit and vegetable puree; s2, mixing the whole-bean minced soybean, the rumex hanus whole pulp, the whole-grain minced pulp and the fruit and vegetable primary pulp obtained in the step S1 to obtain whole-bean rumex hanus nutrient plant enzyme primary pulp, and then sequentially performing enzymolysis by combining cellulase with pectinase, amylase, saccharifying enzyme and protease; and S3, taking the whole-bean rumex hanus nutrient plant enzyme primary pulp subjected to enzymolysis in the step S2, performing anaerobic fermentation by using saccharomycetes and / or distiller's yeast under the negative pressure condition of-0.1 to-0.06 MPa, and filtering to obtain the whole-bean rumex hanus nutrient plant enzyme. The invention further provides the whole-bean rumex hanus nutrient plant enzyme. According to the technical scheme, the defects of traditional bean products, rumex hanus food and edible plant enzymes can be overcome.

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.

The invention belongs to the technical field of food processing, and particularly relates to a cereal nutrient solution and a preparation method thereof. The preparation method of the cereal nutrient solution comprises the following steps: S1, gelatinizing corn liquid to form corn gelatinized liquid; s2, high-temperature amylase is added into the corn gelatinized liquid for enzymolysis, and a solution A is obtained; s3, adding saccharifying enzyme into the solution A for enzymolysis to obtain a solution B; s4, cellulase is added into the solution B for enzymolysis, and a solution C is obtained; s5, protease is added into the solution C for enzymolysis, and corn enzymatic hydrolysate is obtained; s6, performing enzyme deactivation treatment on the corn enzymatic hydrolysate, and then performing impurity removal treatment to obtain the cereal nutrient solution. The high-temperature amylase, the saccharifying enzyme, the cellulase and the protein powder enzyme are sequentially added into the corn liquid and are respectively subjected to enzymolysis, so that the finally prepared cereal nutrient solution has original fragrance and is high in nutrient content.

The preparation method comprises the following steps: degreasing polygonatum cyrtonema leaf superfine powder, sequentially carrying out ultrasonic-assisted enzymolysis treatment on cellulase, xylanase, high-temperature-resistant alpha-amylase, papain and saccharifying enzyme, and carrying out alcohol precipitation, dialysis and vacuum freeze drying treatment to obtain the polygonatum cyrtonema leaf polysaccharide. The polygonatum cyrtonema leaf enzyme extraction crude polysaccharide is obtained; and separating the enzyme-extracted crude polysaccharide through a cellulose column, and purifying through a sephadex column to obtain the polygonatum cyrtonema leaf enzyme-extracted polysaccharide. Polysaccharide in polygonatum cyrtonema leaves is extracted through ultrasonic-assisted compound enzyme, single pure polysaccharide is obtained through alcohol precipitation, dialysis, cellulose column chromatography and gel column chromatography, and the purity can reach 99.86%; the obtained enzyme extraction polysaccharide has good anti-cancer activity, can significantly inhibit proliferation of breast cancer cells and colorectal cancer cells, has the characteristics of good biocompatibility, strong specificity, low toxic and side effects and the like, and has wide application prospects.

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The invention discloses a method for brewing high-quality antioxidant Luzhou-flavor liquor in a semi-solid state. The method comprises the following steps: separating glutinous sorghum serving as a wine brewing raw material into sorghum rice and sorghum peel through rice milling, recovering residual starch in the sorghum peel by adopting alpha-amylase and saccharifying enzyme, releasing different tannins by adopting cellulase, and depolymerizing tannin dissolved in the sorghum peel and a solution by adopting laccase to release tannin with different structures, so as to obtain tannin enzymatic hydrolysate; the enzymatic hydrolysate is added into the steamed peeled sorghum rice according to a certain proportion, so that the thermal destructive effect of the steamed material on tannin in the sorghum rice is avoided; and adding yeast for making the white spirit in a semi-solid state to obtain the white spirit which has the antioxidant activity exceeding 80% compared with that of the existing white spirit and meets the national standard of semi-solid Luzhou-flavor white spirit. Compared with the traditional baijiu brewed by steaming sorghum, the total ester content of the baijiu brewed by adding the tannin enzymatic hydrolysate is increased by 87.50-102.27%, the free radical scavenging rate of DPPH is increased by 134.16-158.71%, and the content of guaiacol substances (4-ethyl guaiacol and guaiacol) is increased by 160.69-166.90%.

The invention discloses a germinated brown rice fermentation product filtrate as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, adjusting the pH value of a CaCl2 solution with glutamic acid to obtain a CaCl2 germination accelerating solution, immersing disinfected brown rice into the CaCl2 germination accelerating solution for germination treatment, and grinding to obtain germinated brown rice slurry; s2, adding liquefying enzyme and saccharifying enzyme into the germinated brown rice pulp, and performing enzymolysis to the end point to obtain brown rice saccharified liquid; s3, saccharomyces cerevisiae CGMCC No.33650 is inoculated into a glucose culture medium for aerobic culture, and a seed solution is obtained; s4, inoculating the seed solution into a culture solution containing a brown rice saccharification solution, and carrying out aerobic fermentation to an end point to obtain a fermentation solution; s5, performing centrifugal treatment on the fermentation liquid to obtain germinated brown rice fermentation product filtrate. The filtrate of the fermentation product is rich in GABA, amino acid, protein, small molecular organic acid and phenolic substances, retains mellow wine fragrance and does not contain alcohol; the whitening and brightening effects, the anti-wrinkle and repairing effects, the anti-aging effect, the anti-oxidation effect, the soothing effect, the moisturizing effect and the like are realized.

The invention relates to the technical field of natural plant extraction, and in particular to a method for extracting scutellaria baicalenzyme by pretreatment. The existing scutellaria baicalenzyme extraction technology is improved, and the scutellaria baicalenzyme is pretreated in sequence by amylase, saccharifying enzyme and pectinase, so that the colloid dissolution rate in the extraction process is greatly reduced, thereby increasing the extraction rate of scutellaria baicalenzyme, thereby effectively utilizing wild resources, increasing the utilization rate of raw materials, and improving economic benefits. The pretreated crude product is extracted by a percolation method, which saves the process of separating the extract and the drug residue, reduces energy consumption, greatly reduces the amount of organic reagents, is environmentally friendly, economical, safe, and has a wide market value.

The invention relates to the technical field of food processing and functional beverages, in particular to a preparation technology of wheat germ pulp, the wheat germ pulp and a functional beverage containing the wheat germ pulp. According to the preparation process of the wheat germ pulp provided by the invention, the pulsed electric field is applied in the gelatinization process, a specific enzymolysis sequence of amylase, saccharifying enzyme and cellulase is adopted, and ultrasonic treatment is carried out during first enzymolysis, so that the sugar degree and yield of the wheat germ pulp are remarkably improved, and meanwhile, the pulp viscosity is reduced; the taste of the wheat germ pulp is effectively improved.

The invention discloses a method for preparing high-purity mannan oligosaccharide from konjac fine powder, which comprises the following steps: washing the konjac fine powder with alcohol, treating with alpha-amylase and glucoamylase, degrading konjac glucomannan by using a compound enzyme consisting of beta-glucanase and beta-mannase, and finally adjusting the pH value to an isoelectric point to prepare the high-purity konjac mannan oligosaccharide. According to the method, high-content impurities such as starch, protein and alkaloid in the konjak fine powder are removed, the problem of accumulation control of konjak processing byproducts is solved, the utilization rate and the economic value of konjak resources are improved, and the source of mannan oligosaccharide is enriched. The method realizes recycling of the konjac fine powder, has the advantages of being simple in process, low in equipment requirement and suitable for industrial production, and can be popularized and applied on a large scale.

The invention provides a preparation method of glucose, and belongs to the technical field of glucose. The method comprises the steps of starch milk preparation, liquefaction, saccharification, filtration and post-treatment. The filtering step comprises the following steps: filtering the saccharified liquid through a 0.5 mm screen, collecting primary filtrate of the saccharified liquid, then adding an impurity removal agent, filtering, collecting secondary filtrate of the saccharified liquid, and carrying out nanofiltration treatment to obtain saccharified filtrate; the preparation method of the impurity removal agent comprises the following steps: placing activated carbon in a nitric acid solution, raising the temperature, stirring, filtering out, washing, putting into deionized water, adding a catalyst, stirring and catalyzing at room temperature for 28-32 minutes, then adding lauryl amine and glutamic acid, stirring and reacting at room temperature, filtering, washing, putting into a chitosan solution, stirring at 34-36 DEG C for 38-42 minutes, and filtering to obtain the impurity removal agent. Filtering, washing and drying to obtain an impurity removal agent; by adopting the preparation method disclosed by the invention, the yield and purity of the glucose are high, the stability of the functional saccharifying enzyme is good, and the energy consumption is low.

The invention relates to the technical field of feeds, in particular to a diatomite carrying agent enriched rice polysaccharide feed and a preparation method thereof. The raw materials comprise diatomite, rice, glutinous rice, flour, dry yeast, beer yeast, rhizopus sweet rice wine koji, raw rice flour, glutinous rice flour, brown sugar, soybean meal, saccharifying enzyme, sodium propionate, sodium lactate, astragalus membranaceus, liquorice, angelica sinensis, radix angelicae, clove, malt, momordica grosvenori, codonopsis pilosula and flaccid knotweed herb. The diatomite carrying agent enriched rice polysaccharide feed disclosed by the invention contains extremely rich polysaccharides, and is safe and nutritional; the preparation process of the feed is ecological and environment-friendly, can be fully copied, is easy to prepare, low in price and remarkable in economic benefit, meanwhile, the value of the rice is increased, and the feed has great strategic significance for maintaining food safety.

The present invention provides a method for preparing polygonatum wine, which comprises mixing polygonatum with saccharifying enzyme for saccharification and then mixing with saccharomyces cerevisiae FN107 bacterial liquid for fermentation. Compared with the traditional fermentation koji, the optimized fermentation process has the advantages of fast fermentation speed, convenient process operation, rich in polysaccharides, the main functional component of polygonatum, and can effectively improve the utilization efficiency of polygonatum, enrich the product types of polygonatum, expand the polygonatum industry chain, and thus promote the polygonatum industry.

The invention relates to the field of preparation of rice drinks, in particular to a preparation method of a rice drink, which comprises the following steps: S1, cleaning rice, and soaking for 25 minutes in summer and 35-40 minutes in winter; s2, adding water into the soaked rice according to the material-liquid ratio of 1: 12.5, and smashing the rice into pulp by pulping equipment; s3, adjusting the pH value of the rice milk to 5.0-5.6, and adding high-temperature alpha-amylase for liquefying; s4, adding saccharifying enzyme into the liquefied rice milk for saccharifying; s5, adjusting the pH value of the saccharified raw materials to 6.5-7.5, and adding protease for enzymolysis; s6, the rice milk obtained after enzymolysis is filtered through a 200-mesh filter; s7, the rice milk is compounded with oat juice, and white sugar, an emulsion stabilizer and curdlan are added for blending; s8, the compound mixture is homogenized through a high-pressure homogenizer; s9, performing sterilization treatment on the rice juice beverage; and S10, performing sterile filling on the sterilized rice juice beverage, and converting the rice into the rice beverage which is good in stability and not easy to stratify through a series of process steps.

The invention belongs to the field of biomass-based functional materials, relates to a difunctional coating based on enzymolysis modified cassava starch as well as a preparation method and application of the difunctional coating, and innovatively solves the technical problem that a single coating gives consideration to oil prevention and water absorption through a synergistic technical path of pretreatment and composite enzymolysis. The core mechanism is as follows: the starch particle structure is destroyed through pretreatment, and the degradation degree of starch is accurately controlled through the synergistic effect of alpha-amylase and saccharifying enzyme, so that an unexpected technical effect is achieved, and an enzymolysis product with a film-forming skeleton (medium molecular weight dextrin) and a hydrophilic unit (small molecular saccharide) is formed; meanwhile, a compact and continuous network structure can be formed by a film-forming framework, and grease permeation is blocked; and the hydrophilic unit endows the coating with a rapid capillary water absorption effect.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a fructus forsythiae extract and a preparation method thereof.The method comprises the following steps that 1, pretreatment is conducted, specifically, fructus forsythiae is added into water to be braised, then dried and ground, and fructus forsythiae powder is obtained; (2) adding the fructus forsythiae powder into a deep-eutectic solvent, then adding an enzyme preparation, carrying out enzymolysis extraction under an ultrasonic condition, and after the enzymolysis extraction is finished, filtering out precipitates to obtain a fructus forsythiae extract; wherein the enzyme preparation comprises cellulase, pectinase and saccharifying enzyme; the eutectic solvent is prepared from choline chloride, glycerol and polyethylene glycol in a molar ratio of 1: 1: (2-3). The fructus forsythiae extract obtained by the method has a very high elimination rate on DPPH free radicals, superoxide anion free radicals and ABTS free radicals, and can be applied to antioxidant products as a raw material.

The invention discloses a method for producing phloretin by using saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. A natural metabolic phloretin path in a plant is successfully constructed in the saccharomyces cerevisiae BY4741, synthesis of phloretin in microorganisms is achieved, synthesis of the phloretin in the microorganisms is enhanced by enhancing synthesis path genes of tyrosine and phenylalanine in the saccharomyces cerevisiae, including elimination of tyrosine feedback inhibition and introduction of exogenous genes, synthesis of prerequisite tyrosine and phenylalanine is enhanced, and the yield of the phloretin is increased. And the yield of the phloretin is further improved by enhancing the expression of a key gene in a phloretin synthesis pathway. The yield of phloretin synthesized from the beginning by the engineering yeast can reach 246.21 mg / L, the synthesis problem of phloretin serving as a carbohydrase inhibitor is effectively solved, and a foundation is laid for synthesizing phloretin through efficient fermentation of microorganisms.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

The invention belongs to the technical field of biology, and relates to a method for preparing peony seed protein from an oil peony byproduct. The peony seed protein is obtained by taking oil peony seed meal as a raw material and adopting a separation and purification method of protease enzymolysis, saccharifying enzyme desugaring, centrifugation, microfiltration and ultrafiltration. The peony seed protein disclosed by the invention is low in alkali consumption and dosage, simple to operate and high in solubility.

The invention provides radix puerariae and cordyceps sinensis wine and a brewing process thereof, and belongs to the technical field of health care wine. The brewing process comprises the following steps: mixing sorghum and kudzuvine root which are cleaned and soaked for 1-2 days, crushing, adding water for gelatinization, adding alpha amylase into the gelatinized sorghum and kudzuvine root for liquefying, and adding saccharifying enzyme for saccharifying; drying the cordycepin donor to constant weight at 50-60 DEG C, and crushing; adding activated saccharomycetes, cordycepin donor powder and vinasse into the saccharified liquid which is cooled to 20-35 DEG C, uniformly stirring, and jointly fermenting; and distilling the fermented wine material to obtain the radix puerariae and cordyceps sinensis wine. According to the brewing process, high-value utilization of the cordyceps sinensis waste compost is achieved, effective components in the cordyceps sinensis culture medium are reserved to the maximum extent, and the brewed radix puerariae and cordyceps sinensis wine is rich in taste, clear in gradation and remarkable in health care effect.

The application discloses a method for preparing an oat-based beverage through biological enzymolysis and a product thereof, and comprises the following steps: continuously hydrolyzing starch, cellulose, protein and other substances in oat slurry by using high-temperature-resistant amylase, cellulase, saccharifying enzyme, papain and alkaline protease, sufficiently releasing reducing substances in the oat, simultaneously generating reducing peptides, improving the free radical scavenging capacity of the oat slurry, and improving the emulsification stability of the oat slurry due to the moderate degradation of macromolecular substances such as starch, cellulose and protein; glycerol is added in three stages at a controlled speed, so that the glycerol is fully mixed with flaxseed oil, and excessive glycerol is avoided from adhering to lipase to reduce the activity of the enzyme, thereby improving the reaction efficiency.

A porous biological polymerizing agent for sediment dewatering in environmental dredging of rivers and lakes is disclosed, which is obtained by thoroughly mixing 50 wt % to 70 wt % of an agent A and 30 wt % to 50 wt % of an agent B into irregular spheres of 1 mm to 3 mm, and crushing the irregular spheres into solid particles with a particle size of ≤20 mesh, and the solid particles have a pH of 5.0 to 6.0. The agent A is obtained by thoroughly mixing 10 wt % to 30 wt % of cellulose, 20 wt % to 50 wt % of starch, and 20 wt % to 40 wt % of amino acid; and the agent B is obtained by thoroughly mixing 40 wt % to 70 wt % of saccharifying enzyme (SE) and 30 wt % to 60 wt % of citric acid.

The application discloses a method for improving the protein secretion capacity of Aspergillus and a recombinant strain obtained from the method. An16g07795 The method comprises performing genetic operation on Aspergillus to delete or reduce the activity of specific genes An16g07795 , so as to improve the protein secretion capacity of saccharifying enzyme, alpha-amylase, other proteins participating in starch hydrolysis and the like. The application utilizes the CRISPR-Cas9 genome editing technology to knock out the genes of Aspergillus niger, obtains a gene editing deletion mutant strain, and significantly improves the production level of starch hydrolysis enzymes, including the protein secretion level and the activity of saccharifying enzyme, so the application has great application value.