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197 results about "Carbohydrase" patented technology

Carbohydrase is a set of enzymes that catalyzes 5 types of breakdown, turning carbohydrates into simple sugars. Carbohydrases are produced in the pancreas and salivary glands, breaking down polysaccharides. This is because complex sugars are often insoluble (such as starch), and therefore breaking them down will make it easier for the sugars to be absorbed into the blood, through the wall of the small intestine. A carbohydrate is usually a compound consisting of carbon, hydrogen and oxygen. Carbohydrase facilitates the hydrolysis of polysaccharides into simpler sugars called Monosaccharides.

Aspergillus oryzae ZA307 and application thereof

The invention relates to the technical field of microorganisms, in particular to an Aspergillus oryzae ZA307 strain and application of the Aspergillus oryzae ZA307 strain. The aspergillus oryzae ZA307 high-yield protease and aminopeptidase provided by the invention cooperate with aspergillus oryzae Huniang 3.042 for starter propagation, so that the activity of the protease, aminopeptidase, saccharifying enzyme and amylase of a starter material can be remarkably improved, and furthermore, the content of ammonia nitrogen and total nitrogen and the variety and content of volatile flavor substances in a product are remarkably improved. The soy sauce or soybean paste prepared and fermented by double-strain starter propagation is obviously improved in the aspects of sauce fragrance, ester fragrance, delicate flavor and harmonious taste, and the quality of the product is comprehensively improved.
Owner:FOSHAN HAITIAN GAOMING FLAVORING & FOOD +2

Method for preparing D-mannose through catalysis

The method for preparing D-mannose through catalysis can effectively reduce the amount of by-products glucose and fructose, and greatly improves the conversion rate of D-mannose. Specifically, engineering bacteria for expressing isoamylase genes and engineering bacteria for expressing alpha-glucan phosphorylase genes, glucophosphate mutase genes, difunctional enzyme glucophosphate isomerase / mannose 6-phosphate isomerase genes and mannose 6-phosphate phosphatase genes are utilized, starch or starch derivatives are used as substrates, and the starch or starch derivatives are used as substrates. A phosphate buffer solution and Mg < 2 + > are added, a preliminary catalysis system is constructed, after the reaction is completed, an incompletely-reacted substrate and maltodisaccharide exist in the reaction system, the incompletely-reacted substrate and maltodisaccharide can be hydrolyzed into glucose by glucoamylase, and the polyphosphoglucokinase can be used for catalyzing the reaction of the polyphosphoglucokinase under the assistance of sodium hexametaphosphate. All glucose is converted into phosphorylated glucose, then D-mannose is generated, and by-products in a system are removed while the yield is increased.
Owner:BINZHOU SANYUAN BIOLOGICAL TECH

Whole-bean rumex hanus nutrient plant enzyme and preparation method thereof

The invention discloses a preparation method of a whole-bean rumex hanus nutrient plant enzyme, which comprises the following steps: S1, taking soybeans, and preparing whole-bean minced soybean; taking rumex hanus to prepare rumex hanus whole pulp; taking grains, and preparing whole grain pulp paste; taking fruits and vegetables, and preparing fruit and vegetable puree; s2, mixing the whole-bean minced soybean, the rumex hanus whole pulp, the whole-grain minced pulp and the fruit and vegetable primary pulp obtained in the step S1 to obtain whole-bean rumex hanus nutrient plant enzyme primary pulp, and then sequentially performing enzymolysis by combining cellulase with pectinase, amylase, saccharifying enzyme and protease; and S3, taking the whole-bean rumex hanus nutrient plant enzyme primary pulp subjected to enzymolysis in the step S2, performing anaerobic fermentation by using saccharomycetes and / or distiller's yeast under the negative pressure condition of-0.1 to-0.06 MPa, and filtering to obtain the whole-bean rumex hanus nutrient plant enzyme. The invention further provides the whole-bean rumex hanus nutrient plant enzyme. According to the technical scheme, the defects of traditional bean products, rumex hanus food and edible plant enzymes can be overcome.
Owner:HEBEI ZHENDOU FOOD CO LTD +1

Distilled liquor added with bamboo leaf powder and preparation method of distilled liquor

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.
Owner:NAT FORESTRY & GRASSLAND ADMINISTRATION BAMBOO RES & DEV CENT

Germinated brown rice fermentation product filtrate as well as preparation method and application thereof

The invention discloses a germinated brown rice fermentation product filtrate as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, adjusting the pH value of a CaCl2 solution with glutamic acid to obtain a CaCl2 germination accelerating solution, immersing disinfected brown rice into the CaCl2 germination accelerating solution for germination treatment, and grinding to obtain germinated brown rice slurry; s2, adding liquefying enzyme and saccharifying enzyme into the germinated brown rice pulp, and performing enzymolysis to the end point to obtain brown rice saccharified liquid; s3, saccharomyces cerevisiae CGMCC No.33650 is inoculated into a glucose culture medium for aerobic culture, and a seed solution is obtained; s4, inoculating the seed solution into a culture solution containing a brown rice saccharification solution, and carrying out aerobic fermentation to an end point to obtain a fermentation solution; s5, performing centrifugal treatment on the fermentation liquid to obtain germinated brown rice fermentation product filtrate. The filtrate of the fermentation product is rich in GABA, amino acid, protein, small molecular organic acid and phenolic substances, retains mellow wine fragrance and does not contain alcohol; the whitening and brightening effects, the anti-wrinkle and repairing effects, the anti-aging effect, the anti-oxidation effect, the soothing effect, the moisturizing effect and the like are realized.
Owner:BEIJING UNIV OF CHEM TECH +1

Preparation process of wheat germ pulp, wheat germ pulp and functional beverage containing wheat germ pulp

The invention relates to the technical field of food processing and functional beverages, in particular to a preparation technology of wheat germ pulp, the wheat germ pulp and a functional beverage containing the wheat germ pulp. According to the preparation process of the wheat germ pulp provided by the invention, the pulsed electric field is applied in the gelatinization process, a specific enzymolysis sequence of amylase, saccharifying enzyme and cellulase is adopted, and ultrasonic treatment is carried out during first enzymolysis, so that the sugar degree and yield of the wheat germ pulp are remarkably improved, and meanwhile, the pulp viscosity is reduced; the taste of the wheat germ pulp is effectively improved.
Owner:北京再益生物科技有限公司

Diatomite carrying agent enriched rice polysaccharide feed and preparation method thereof

The invention relates to the technical field of feeds, in particular to a diatomite carrying agent enriched rice polysaccharide feed and a preparation method thereof. The raw materials comprise diatomite, rice, glutinous rice, flour, dry yeast, beer yeast, rhizopus sweet rice wine koji, raw rice flour, glutinous rice flour, brown sugar, soybean meal, saccharifying enzyme, sodium propionate, sodium lactate, astragalus membranaceus, liquorice, angelica sinensis, radix angelicae, clove, malt, momordica grosvenori, codonopsis pilosula and flaccid knotweed herb. The diatomite carrying agent enriched rice polysaccharide feed disclosed by the invention contains extremely rich polysaccharides, and is safe and nutritional; the preparation process of the feed is ecological and environment-friendly, can be fully copied, is easy to prepare, low in price and remarkable in economic benefit, meanwhile, the value of the rice is increased, and the feed has great strategic significance for maintaining food safety.
Owner:GUANGZHOU ZHENHUA JUNHUI TECHNOLOGY CO LTD +1

Difunctional coating based on enzymolysis modified cassava starch as well as preparation method and application of difunctional coating

The invention belongs to the field of biomass-based functional materials, relates to a difunctional coating based on enzymolysis modified cassava starch as well as a preparation method and application of the difunctional coating, and innovatively solves the technical problem that a single coating gives consideration to oil prevention and water absorption through a synergistic technical path of pretreatment and composite enzymolysis. The core mechanism is as follows: the starch particle structure is destroyed through pretreatment, and the degradation degree of starch is accurately controlled through the synergistic effect of alpha-amylase and saccharifying enzyme, so that an unexpected technical effect is achieved, and an enzymolysis product with a film-forming skeleton (medium molecular weight dextrin) and a hydrophilic unit (small molecular saccharide) is formed; meanwhile, a compact and continuous network structure can be formed by a film-forming framework, and grease permeation is blocked; and the hydrophilic unit endows the coating with a rapid capillary water absorption effect.
Owner:SHANGHAI ZIDAN FOOD PACKAGING & PRINTING CO LTD +1

High-purity resistant dextrin and preparation method thereof

PendingCN122060814AAchieve completely green biosynthesisIncrease contentFermentationAmylaseFermentation
The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.
Owner:SOUTH CHINA UNIV OF TECH

Method for preparing peony seed protein from oil peony byproduct

The invention belongs to the technical field of biology, and relates to a method for preparing peony seed protein from an oil peony byproduct. The peony seed protein is obtained by taking oil peony seed meal as a raw material and adopting a separation and purification method of protease enzymolysis, saccharifying enzyme desugaring, centrifugation, microfiltration and ultrafiltration. The peony seed protein disclosed by the invention is low in alkali consumption and dosage, simple to operate and high in solubility.
Owner:JINAN NEW SIWEI BIOTECHNOLOGY CO LTD

A method for preparing an oat-based beverage by bioenzymatic hydrolysis and products thereof

ActiveCN118435999BBiotechnologyAmylase
The application discloses a method for preparing an oat-based beverage through biological enzymolysis and a product thereof, and comprises the following steps: continuously hydrolyzing starch, cellulose, protein and other substances in oat slurry by using high-temperature-resistant amylase, cellulase, saccharifying enzyme, papain and alkaline protease, sufficiently releasing reducing substances in the oat, simultaneously generating reducing peptides, improving the free radical scavenging capacity of the oat slurry, and improving the emulsification stability of the oat slurry due to the moderate degradation of macromolecular substances such as starch, cellulose and protein; glycerol is added in three stages at a controlled speed, so that the glycerol is fully mixed with flaxseed oil, and excessive glycerol is avoided from adhering to lipase to reduce the activity of the enzyme, thereby improving the reaction efficiency.
Owner:WUXI CHENGBAO FOODS CO LTD

Preparation method of quinoa and red date yoghurt

PendingCN122623724ABiotechnologyAmylase
The present application relates to the field of food processing, and particularly relates to a preparation method of quinoa and red date yoghurt. The method comprises the following steps: quinoa is soaked, ground, and treated by gradient temperature enzyme inactivation, and then mixed with red date pulp at a volume ratio of 1:1, and then treated by alpha-amylase liquefaction, saccharifying enzyme and pullulanase compound saccharification, and low-oxygen flavor pre-hybridization, and then mixed with reconstituted milk and compound stabilizer konjac glucomannan and sodium pyrophosphate, and then sterilized, cooled, inoculated with starter culture, and then fermented and ripened to obtain the product. The present application eliminates the peculiar smell of quinoa by gradient enzyme inactivation, and saccharifying enzyme and pullulanase have a significant synergistic effect in the quinoa and red date yoghurt system, and the secondary enzymolysis of liquefaction and saccharification significantly improves the starch utilization rate, and the compounding of konjac glucomannan and sodium pyrophosphate significantly improves the product texture, and the present application solves the technical problems of low starch utilization rate, obvious peculiar smell, poor water holding capacity, and low viable count of traditional quinoa yoghurt.
Owner:BOZHOU UNIV

Dynamic optimization process for filtration parameters of nanofiltration membrane of glucose mother liquor

The invention relates to the technical field of membrane separation, in particular to a glucose mother liquor nanofiltration membrane filtration parameter dynamic optimization process which comprises mother liquor pretreatment, intelligent nanofiltration filtration, clear liquid treatment, residual liquid deep saccharification and finished product preparation. A sulfonated chitosan-graphene modifier is added during pretreatment, a polyamide membrane is adopted for nanofiltration, the temperature, pressure and flow velocity are dynamically adjusted through a PLC-AI system, a TiO-ZnO nano liquid is synchronously added, clear liquid is concentrated, composite saccharifying enzyme is added for reaction, enzyme is added into residual liquid, nano aerogel is saccharified, and a finished product is dried through pulse hot air circulation. According to the process, AI dynamic regulation and control are combined with a nano material, so that the pollution resistance of a nanofiltration membrane is improved, the purity of glucose is improved to 92% or above, the yield is higher than 89%, oligosaccharide residues in saccharified liquid are reduced, drying energy consumption and COD emission are reduced, and high-value utilization of mother liquor is realized.
Owner:QIQIHAR LONGJIANG FUFENG BIOTECHNOLOGIES CO LTD

Preparation method of rice bran fermentation product filtrate as well as product and application thereof

The invention provides a preparation method of rice bran fermentation product filtrate as well as a product and application thereof. The preparation method comprises the following steps: (1) sterilizing rice bran, mixing the sterilized rice bran with compound enzyme and water, adjusting the pH value, performing enzymolysis reaction, and performing enzyme deactivation after the enzymolysis reaction is finished to obtain rice bran zymolyte; the compound enzyme comprises alpha-amylase, beta-xylosidase and saccharifying enzyme; (2) mixing the rice bran zymolyte, an auxiliary carbon source and water to prepare a fermentation substrate; and (3) inoculating compound bacteria into the fermentation substrate for fermentation, sterilizing after fermentation, and carrying out solid-liquid separation to obtain the rice bran fermentation product filtrate. According to the method, active ingredients in the rice bran can be fully and effectively extracted and utilized, the stability and the absorption rate of the rice bran fermentation product filtrate are improved, and the prepared rice bran fermentation product filtrate contains rich effective ingredients such as ceramide, vitamins, polyphenol compounds and trace elements; the composition has excellent soothing, repairing and anti-oxidation effects.
Owner:GUANGZHOU SHANHE CHEM CO LTD

Preparation method and application of high-palatability carrot dietary fiber

The invention provides a preparation method and application of high-palatability carrot dietary fibers. The method comprises the following steps: (1) washing and dicing carrots; (2) cellulase and saccharifying enzyme enzymolysis; (3) constructing a two-phase system, adding a compound enzyme and pectinase, and carrying out gradient enzymolysis; (4) carrying out enzyme deactivation and centrifugation to obtain a water phase; (5) performing gradient centrifugation to obtain mixed fibers; (6) precipitating, and collecting precipitated fibers; (7) adding carrot source nano cellulose to obtain a composite fiber with a core-shell structure; and (8) drying, crushing and sieving. According to the preparation method disclosed by the invention, the core problems of rough taste (large particle size), astringent taste (residual acetyl groups and terpene substances), weak function and low purity in the traditional carrot dietary fiber preparation process are solved through a water-alcohol two-phase gradient composite enzymolysis and nano cellulose composite purification process; and finally, the high-palatability carrot dietary fiber which is fine, smooth, free of rough feeling, natural, fresh and sweet, high in purity and strong in function is prepared.
Owner:HUAZHONG AGRI UNIV

A method for preparing fermented Chinese herbal wine

This invention discloses a method for preparing fermented Chinese medicine wine, comprising the following steps: S1: Preparing brewing grains by weight, soaking, steaming, and treating with saccharifying enzymes to obtain saccharified grains; S2: Treating Chinese medicinal materials with compound enzymes to obtain enzymatically hydrolyzed Chinese medicinal materials; S3: Mixing the saccharified grains and enzymatically hydrolyzed Chinese medicinal materials, adding yeast for anaerobic fermentation to obtain fermented material; S4: Distilling and aging the fermented material to obtain fermented Chinese medicine wine. The advantages of this invention compared to existing technologies are: providing a method for preparing fermented Chinese medicine wine that achieves efficient release and full conversion of the effective components of Chinese medicinal materials, ensuring a mellow flavor and stable quality.
Owner:CHENGZE YAOJUNTANG TRADITIONAL CHINESE MEDICINE CLINIC (CHONGQING) CO LTD

A compound koji inoculated with indigenous aspergillus oryzae and aspergillus niger and a method for strengthening shanxi old mature vinegar

The application belongs to the technical field of vinegar brewing, and provides a compound koji inoculated with indigenous Aspergillus oryzae and Aspergillus niger and a method for strengthening Shanxi old mature vinegar. Indigenous Aspergillus oryzae CGMCC 42103 with high yield of acid protease and glutaminease and Aspergillus niger CGMCC 15672 with high yield of saccharifying enzyme are mixed and inoculated in bran and soybean powder medium to prepare compound koji, and the prepared compound koji is applied to the fermentation process of Shanxi old mature vinegar to prepare new vinegar, the total acid, amino acid nitrogen, non-volatile acid and glutamic acid content of the new vinegar are 6.18 g / 100 mL, 0.38 g / 100 mL, 2.45 g / 100 mL and 58.83 mg / 100 mL respectively, compared with the comparative example in which only highland millet with a mass fraction of 60% is added in the alcohol fermentation stage to carry out conventional fermentation of food vinegar, the new vinegar is improved by 20.47%, 153.33%, 33.15% and 118.86% respectively, and the content of organic acid and volatile aroma components is enriched.
Owner:JINZHONG COMPREHENSIVE INSPECTION & TESTING CENT

A process for the catalytic preparation of D-mannose

The method for catalytically preparing D-mannose provided in the application can effectively reduce the amount of by-products glucose and fructose, and greatly improve the conversion rate of D-mannose. Specifically, the engineering bacteria expressing isoamylase gene and the engineering bacteria expressing alpha-glucan phosphorylase gene, glucose phosphomutase gene, bifunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and mannose 6-phosphate phosphatase gene are used, starch or starch derivatives are used as substrates, phosphate buffer solution and Mg 2+ A preliminary catalytic system is constructed, and after the reaction is completed, there are unreacted substrates and maltodextrin in the reaction system, the saccharifying enzyme can hydrolyze the unreacted substrates and maltodextrin into glucose, the polyphosphate glucose kinase can convert all the glucose into phosphorylated glucose with the assistance of sodium hexametaphosphate, and then D-mannose is generated, thereby improving the yield and removing the by-products in the system.
Owner:BINZHOU SANYUAN BIOLOGICAL TECH

Method for preparing structured starch coating liquid with oil-proof and moisture-absorbing dual functions

The present application relates to the technical field of starch coating, in particular to a preparation method of structured starch coating liquid with oil-proof and moisture-absorbing dual functions. The method comprises the following steps: firstly, performing mild acidolysis or selective oxidation pretreatment on cassava starch; then, preparing starch milk by using the pretreated starch, and performing synergistic enzymolysis on the starch milk under mild conditions by using a composite enzyme composed of alpha-amylase and glucoamylase in a specific ratio; after the enzymolysis, performing enzyme inactivation treatment, and then adding a bio-based crosslinking agent for compounding. The prepared coating has a specific bimodal distribution structure of starch derivative molecular weight, which is a key to realize function balance. After the coating liquid is coated on a substrate such as paper and dried and solidified, the formed coating not only has excellent oil-proof performance, but also exhibits good water vapor absorption capacity. The raw materials of the present application are green and the process is environmentally friendly, and the prepared multifunctional coating can be effectively applied to the fields of food packaging, medical protection and other fields which need to resist oil and regulate humidity at the same time.
Owner:JIANGSU XUSHENG ENVIRONMENTAL PROTECTION TECH CO LTD

Method of improving carbohydrate digestibility by a carbohydratase in an animal feed by employing serine protease

PendingUS20250318552A1Animal feeding stuffAccessory food factorsWheat middlingsSorghum
The present invention provides a method for improving the digestibility of a carbohydrate by a carbohydrase in an animal feed such as soybean-meal, corn, wheat, wheat middlings, oats, rye, barley and sorghum; and / or roughages such as hay and pasture plants, or mixture thereof by adding one or more proteases, preferably a serine protease such as a Nocardiopsis serine protease or a S8 serine protease. The present invention also provides a feed composition suitable for the above methods.
Owner:NOVONESIS ANIMAL BIOSOLUTIONS AG +1

Saccharifying tank for white spirit production

The utility model discloses a saccharifying tank for white spirit production in the technical field of saccharifying tanks, which comprises a storage tank, two stirring rings are arranged in the storage tank, the saccharifying tank further comprises a driving unit and a gas supply unit, the driving unit is used for driving the two stirring rings to move along the length direction of the storage tank and controlling the two stirring rings to rotate, and the gas supply unit is used for supplying gas to the storage tank. And the air supply unit is used for providing air for the surfaces of the two stirring rings. According to the storage tank, the two stirring rings are arranged in the storage tank body, when the storage tank is used, a user places raw materials and glucoamylase in the storage tank body, then the driving unit controls the two stirring rings to move in the length direction of the storage tank body, and in the process, the two stirring rings rotate synchronously; the air supply unit can spray air to the surfaces of the two stirring rings, so that oxygen can be uniformly sprayed into the raw materials and the saccharifying enzyme, and the saccharifying efficiency is effectively improved.
Owner:SHANXI YU CHENGLONG LIQUOR CO LTD

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

PendingUS20250236875A1BiofuelsFermentationStarch cornGranular starch
The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
Owner:STELLENBOSCH UNIVERSITY +1

Stable liquid sourdough products

The present application provides methods for preparing a liquid sourdough product comprising: a) adding amyloglucosidase to a liquid mixture comprising cereal(s) and / or cereal fractions; b) inoculating the liquid mixture with one or more strains of Weissella confusa; c) fermenting the liquid mixture obtained in step b) for a period between 4 and 8 hours at a temperature between 15.0 and 40.0°C; d) adding at least 5.0 % (w / w) of sugar to the fermented liquid mixture obtained in step c); and e) further fermenting the fermented liquid mixture obtained in step d) at a temperature between 15.0 and 40.0°C for a period between 18 and 48 hours, thereby obtaining a liquid sourdough product. The application further provides stable liquid sourdough products obtained by such methods and uses thereof.
Owner:PURATOS NV

Preparation method of glucosyl puerarin

The invention discloses a preparation method of glucosyl puerarin, which belongs to the technical field of pharmaceutical chemicals, and comprises the following steps: S1, adding sugar for reaction, mixing puerarin with pure water, adjusting the pH value to 4.5-5.5, adding beta-cyclodextrin, heating to 45-55 DEG C, adding cyclodextrin glucosyltransferase for reaction for 16-22 hours, then adjusting the pH value to 0.1-0.8, adding activated carbon, filtering filtrate and staying overnight at low temperature; s2, desugaring reaction: adjusting the pH value of the filtrate to 3.8-4.5, heating to 55-65 DEG C, adding saccharifying enzyme and glucose oxidase to react for 25-40 minutes, adjusting the pH value to 0.5-1.2, and filtering; s3, column purification: enabling a reaction solution to pass through a mixed column containing sulfonated modified XAD-16 and amino modified HPD-720 resin, and performing graded elution to collect an ethanol eluent; s4, concentrating and drying to obtain a product. The problems that the water solubility of puerarin is poor, the purity of glucosyl puerarin is low, and impurities are difficult to remove can be solved.
Owner:MIANYANG SHANYI BIOTECHNOLOGY CO LTD

Method for improving the ability of broiler chickens to resist lps stress

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.
Owner:WUHAN SUNHY BIOLOGICAL +1

Method for detecting resistant starch in rice

The invention discloses a rice resistant starch detection method, and belongs to the technical field of resistant starch detection. The rice resistant starch detection method comprises the following steps: grinding rice into rice flour, degreasing the rice flour, adding water, and steaming to obtain cooked rice flour; adding a mixed enzyme buffer solution containing alpha-amylase and glucoamylase into the cured rice flour for treatment, then adding an ethanol solution to terminate the reaction, and centrifuging to obtain a precipitate; adding alkali liquor into the precipitate for treatment, then adding a saccharifying enzyme buffer solution for treatment, and taking supernate after centrifugation; and detecting the content of glucose by using a DNS method, and calculating the content of resistant starch. The method solves the problems that in the prior art, the determination time is long, needed reagents are many and complex, the determination is influenced by starch fermentation due to too long decomposition time of non-resistant starch, the sample pretreatment mode is fuzzy and the like, and the resistant starch content of the rice in the cooking and eating state is accurately reflected.
Owner:YIBIN UNIV +2

Expansin recombinant aspergillus niger suitable for liquid state fermentation and construction method and application of expansin recombinant aspergillus niger

PendingCN121227536AFungiMicroorganism based processesPenicillium oxalicumAspergillus niger
The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Camellia oil emulsification production process

The present invention relates to the technical field of emulsification processes, and particularly to the production process of camellia oil emulsification; it includes the following steps: Step 1: Mix tapioca starch and water to obtain a starch milk; Step 2: Add glucoamylase and α-amylase to the starch milk, add Bacillus firmus for fermentation reaction, after the reaction, inactivate Bacillus firmus, glucoamylase, and α-amylase to obtain a fermentation product; Step 3: Mix the fermentation product, camellia oil, sorbitan monostearate, L-ascorbic acid palmitate, cocoa butter, water, and lecithin according to the mass ratio to obtain a mixture, heat the mixture to 40-45 °C, adjust the pH to 6-8, and circulate and process it 3-5 times under a pressure of 90-100 MPa through a high-pressure homogenizer to obtain a homogeneous emulsion. The prepared camellia oil emulsification product can still maintain a good effect on gastric mucosa repair after being stored under indoor light conditions at room temperature for more than 24 months.
Owner:NINGDE JIUZHAN AGRI CO LTD

Brewing process of medicinal and edible yellow wine and liqueur

The invention discloses a brewing technology of medicinal and edible yellow wine and liqueur, and belongs to the technical field of yellow wine brewing. By adding the dried red dates, the saccharification rate and the health-care function are improved by utilizing natural sugar and active ingredients of the dried red dates; in the saccharification process, high-temperature-resistant alpha-amylase is added in stages, the temperature is accurately controlled, starch is rapidly degraded in cooperation with liquid saccharifying enzyme, the fermentation stage is subjected to aerobic / anaerobic staged control in combination with composite strains such as red yeast rice and rhizopus, generation of functional metabolites is promoted, meanwhile, a medicinal material extracting solution soaked in high-concentration white spirit is added, and the high-temperature-resistant starch is obtained. The efficient fusion and flavor balance of medicinal and edible components are realized, and the final finished product has a mellow taste and a health-preserving effect.
Owner:HESHUN COUNTY JINGSHENG RESEARCH & DEVELOPMENT CO LTD

Corynespora aspera CYPJ1 and application thereof

The invention discloses Corynespora aspera CYPJ1 and an application of the Corynespora aspera CYPJ1. The preservation number of the corynespora aspera CYPJ1 is CGMCC (China General Microbiological Culture Collection Center) No.35719, and the saccharifying enzyme activity of the corynespora aspera CYPJ1 is 73.19 + / Raw materials are homogenized, the content of soluble dietary fibers prepared through fermentation of the strain is increased, and physical characteristics and functional characteristics are enhanced. The raw materials are homogenized, and food or health care products fermented by the strain have the effect of reducing blood fat. The invention also finds that after the raw materials are homogenized, the combined fermentation of clavipora lusitaniae CYPJ1 and kluyveromyces marxianus SXJ2 or bacillus siamensis DL-RXL01 is adopted, so that the effect is better. The invention also finds that after the raw materials are homogenized, the fermentation is carried out by adopting the combination of the clavipora lusitaniae CYPJ1 and the kluyveromyces marxianus SXJ2 or the bacillus siamensis DL-RXL01.
Owner:JILIN ACAD OF AGRI SCI