147 results about "Carbohydrase" patented technology

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The invention relates to the technical field of food processing and functional beverages, in particular to a preparation technology of wheat germ pulp, the wheat germ pulp and a functional beverage containing the wheat germ pulp. According to the preparation process of the wheat germ pulp provided by the invention, the pulsed electric field is applied in the gelatinization process, a specific enzymolysis sequence of amylase, saccharifying enzyme and cellulase is adopted, and ultrasonic treatment is carried out during first enzymolysis, so that the sugar degree and yield of the wheat germ pulp are remarkably improved, and meanwhile, the pulp viscosity is reduced; the taste of the wheat germ pulp is effectively improved.

The invention belongs to the field of biomass-based functional materials, relates to a difunctional coating based on enzymolysis modified cassava starch as well as a preparation method and application of the difunctional coating, and innovatively solves the technical problem that a single coating gives consideration to oil prevention and water absorption through a synergistic technical path of pretreatment and composite enzymolysis. The core mechanism is as follows: the starch particle structure is destroyed through pretreatment, and the degradation degree of starch is accurately controlled through the synergistic effect of alpha-amylase and saccharifying enzyme, so that an unexpected technical effect is achieved, and an enzymolysis product with a film-forming skeleton (medium molecular weight dextrin) and a hydrophilic unit (small molecular saccharide) is formed; meanwhile, a compact and continuous network structure can be formed by a film-forming framework, and grease permeation is blocked; and the hydrophilic unit endows the coating with a rapid capillary water absorption effect.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

The invention belongs to the technical field of biology, and relates to a method for preparing peony seed protein from an oil peony byproduct. The peony seed protein is obtained by taking oil peony seed meal as a raw material and adopting a separation and purification method of protease enzymolysis, saccharifying enzyme desugaring, centrifugation, microfiltration and ultrafiltration. The peony seed protein disclosed by the invention is low in alkali consumption and dosage, simple to operate and high in solubility.

The application discloses a method for preparing an oat-based beverage through biological enzymolysis and a product thereof, and comprises the following steps: continuously hydrolyzing starch, cellulose, protein and other substances in oat slurry by using high-temperature-resistant amylase, cellulase, saccharifying enzyme, papain and alkaline protease, sufficiently releasing reducing substances in the oat, simultaneously generating reducing peptides, improving the free radical scavenging capacity of the oat slurry, and improving the emulsification stability of the oat slurry due to the moderate degradation of macromolecular substances such as starch, cellulose and protein; glycerol is added in three stages at a controlled speed, so that the glycerol is fully mixed with flaxseed oil, and excessive glycerol is avoided from adhering to lipase to reduce the activity of the enzyme, thereby improving the reaction efficiency.

The present application relates to the field of food processing, and particularly relates to a preparation method of quinoa and red date yoghurt. The method comprises the following steps: quinoa is soaked, ground, and treated by gradient temperature enzyme inactivation, and then mixed with red date pulp at a volume ratio of 1:1, and then treated by alpha-amylase liquefaction, saccharifying enzyme and pullulanase compound saccharification, and low-oxygen flavor pre-hybridization, and then mixed with reconstituted milk and compound stabilizer konjac glucomannan and sodium pyrophosphate, and then sterilized, cooled, inoculated with starter culture, and then fermented and ripened to obtain the product. The present application eliminates the peculiar smell of quinoa by gradient enzyme inactivation, and saccharifying enzyme and pullulanase have a significant synergistic effect in the quinoa and red date yoghurt system, and the secondary enzymolysis of liquefaction and saccharification significantly improves the starch utilization rate, and the compounding of konjac glucomannan and sodium pyrophosphate significantly improves the product texture, and the present application solves the technical problems of low starch utilization rate, obvious peculiar smell, poor water holding capacity, and low viable count of traditional quinoa yoghurt.

The invention provides a preparation method and application of high-palatability carrot dietary fibers. The method comprises the following steps: (1) washing and dicing carrots; (2) cellulase and saccharifying enzyme enzymolysis; (3) constructing a two-phase system, adding a compound enzyme and pectinase, and carrying out gradient enzymolysis; (4) carrying out enzyme deactivation and centrifugation to obtain a water phase; (5) performing gradient centrifugation to obtain mixed fibers; (6) precipitating, and collecting precipitated fibers; (7) adding carrot source nano cellulose to obtain a composite fiber with a core-shell structure; and (8) drying, crushing and sieving. According to the preparation method disclosed by the invention, the core problems of rough taste (large particle size), astringent taste (residual acetyl groups and terpene substances), weak function and low purity in the traditional carrot dietary fiber preparation process are solved through a water-alcohol two-phase gradient composite enzymolysis and nano cellulose composite purification process; and finally, the high-palatability carrot dietary fiber which is fine, smooth, free of rough feeling, natural, fresh and sweet, high in purity and strong in function is prepared.

This invention discloses a method for preparing fermented Chinese medicine wine, comprising the following steps: S1: Preparing brewing grains by weight, soaking, steaming, and treating with saccharifying enzymes to obtain saccharified grains; S2: Treating Chinese medicinal materials with compound enzymes to obtain enzymatically hydrolyzed Chinese medicinal materials; S3: Mixing the saccharified grains and enzymatically hydrolyzed Chinese medicinal materials, adding yeast for anaerobic fermentation to obtain fermented material; S4: Distilling and aging the fermented material to obtain fermented Chinese medicine wine. The advantages of this invention compared to existing technologies are: providing a method for preparing fermented Chinese medicine wine that achieves efficient release and full conversion of the effective components of Chinese medicinal materials, ensuring a mellow flavor and stable quality.

The application belongs to the technical field of vinegar brewing, and provides a compound koji inoculated with indigenous Aspergillus oryzae and Aspergillus niger and a method for strengthening Shanxi old mature vinegar. Indigenous Aspergillus oryzae CGMCC 42103 with high yield of acid protease and glutaminease and Aspergillus niger CGMCC 15672 with high yield of saccharifying enzyme are mixed and inoculated in bran and soybean powder medium to prepare compound koji, and the prepared compound koji is applied to the fermentation process of Shanxi old mature vinegar to prepare new vinegar, the total acid, amino acid nitrogen, non-volatile acid and glutamic acid content of the new vinegar are 6.18 g / 100 mL, 0.38 g / 100 mL, 2.45 g / 100 mL and 58.83 mg / 100 mL respectively, compared with the comparative example in which only highland millet with a mass fraction of 60% is added in the alcohol fermentation stage to carry out conventional fermentation of food vinegar, the new vinegar is improved by 20.47%, 153.33%, 33.15% and 118.86% respectively, and the content of organic acid and volatile aroma components is enriched.

The method for catalytically preparing D-mannose provided in the application can effectively reduce the amount of by-products glucose and fructose, and greatly improve the conversion rate of D-mannose. Specifically, the engineering bacteria expressing isoamylase gene and the engineering bacteria expressing alpha-glucan phosphorylase gene, glucose phosphomutase gene, bifunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and mannose 6-phosphate phosphatase gene are used, starch or starch derivatives are used as substrates, phosphate buffer solution and Mg 2+ A preliminary catalytic system is constructed, and after the reaction is completed, there are unreacted substrates and maltodextrin in the reaction system, the saccharifying enzyme can hydrolyze the unreacted substrates and maltodextrin into glucose, the polyphosphate glucose kinase can convert all the glucose into phosphorylated glucose with the assistance of sodium hexametaphosphate, and then D-mannose is generated, thereby improving the yield and removing the by-products in the system.

The present application relates to the technical field of starch coating, in particular to a preparation method of structured starch coating liquid with oil-proof and moisture-absorbing dual functions. The method comprises the following steps: firstly, performing mild acidolysis or selective oxidation pretreatment on cassava starch; then, preparing starch milk by using the pretreated starch, and performing synergistic enzymolysis on the starch milk under mild conditions by using a composite enzyme composed of alpha-amylase and glucoamylase in a specific ratio; after the enzymolysis, performing enzyme inactivation treatment, and then adding a bio-based crosslinking agent for compounding. The prepared coating has a specific bimodal distribution structure of starch derivative molecular weight, which is a key to realize function balance. After the coating liquid is coated on a substrate such as paper and dried and solidified, the formed coating not only has excellent oil-proof performance, but also exhibits good water vapor absorption capacity. The raw materials of the present application are green and the process is environmentally friendly, and the prepared multifunctional coating can be effectively applied to the fields of food packaging, medical protection and other fields which need to resist oil and regulate humidity at the same time.

The present application provides methods for preparing a liquid sourdough product comprising: a) adding amyloglucosidase to a liquid mixture comprising cereal(s) and / or cereal fractions; b) inoculating the liquid mixture with one or more strains of Weissella confusa; c) fermenting the liquid mixture obtained in step b) for a period between 4 and 8 hours at a temperature between 15.0 and 40.0°C; d) adding at least 5.0 % (w / w) of sugar to the fermented liquid mixture obtained in step c); and e) further fermenting the fermented liquid mixture obtained in step d) at a temperature between 15.0 and 40.0°C for a period between 18 and 48 hours, thereby obtaining a liquid sourdough product. The application further provides stable liquid sourdough products obtained by such methods and uses thereof.

The invention discloses a preparation method of glucosyl puerarin, which belongs to the technical field of pharmaceutical chemicals, and comprises the following steps: S1, adding sugar for reaction, mixing puerarin with pure water, adjusting the pH value to 4.5-5.5, adding beta-cyclodextrin, heating to 45-55 DEG C, adding cyclodextrin glucosyltransferase for reaction for 16-22 hours, then adjusting the pH value to 0.1-0.8, adding activated carbon, filtering filtrate and staying overnight at low temperature; s2, desugaring reaction: adjusting the pH value of the filtrate to 3.8-4.5, heating to 55-65 DEG C, adding saccharifying enzyme and glucose oxidase to react for 25-40 minutes, adjusting the pH value to 0.5-1.2, and filtering; s3, column purification: enabling a reaction solution to pass through a mixed column containing sulfonated modified XAD-16 and amino modified HPD-720 resin, and performing graded elution to collect an ethanol eluent; s4, concentrating and drying to obtain a product. The problems that the water solubility of puerarin is poor, the purity of glucosyl puerarin is low, and impurities are difficult to remove can be solved.

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.

The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.

The invention discloses Corynespora aspera CYPJ1 and an application of the Corynespora aspera CYPJ1. The preservation number of the corynespora aspera CYPJ1 is CGMCC (China General Microbiological Culture Collection Center) No.35719, and the saccharifying enzyme activity of the corynespora aspera CYPJ1 is 73.19 + / Raw materials are homogenized, the content of soluble dietary fibers prepared through fermentation of the strain is increased, and physical characteristics and functional characteristics are enhanced. The raw materials are homogenized, and food or health care products fermented by the strain have the effect of reducing blood fat. The invention also finds that after the raw materials are homogenized, the combined fermentation of clavipora lusitaniae CYPJ1 and kluyveromyces marxianus SXJ2 or bacillus siamensis DL-RXL01 is adopted, so that the effect is better. The invention also finds that after the raw materials are homogenized, the fermentation is carried out by adopting the combination of the clavipora lusitaniae CYPJ1 and the kluyveromyces marxianus SXJ2 or the bacillus siamensis DL-RXL01.

The invention discloses a method for removing and degrading starch by a compound enzyme system in a pulping process of waste box boards, which comprises alpha-amylase, beta-amylase, glucoamylase, pullulanase and pectinase. OCC white water and OCC paper pulp are subjected to enzymolysis through alpha-amylase and another three kinds of compound enzymes (beta-amylase, pullulanase and glucoamylase) which are compounded (the enzyme activity is conventional enzyme activity), and when the dosage of the compound enzymes is 1000 mL * t <-1 >, the enzymolysis rate of the compound enzymes is far superior to that of single alpha-amylase with the dosage of 5000 mL * t <-1 > at the same enzymolysis time; the starch decomposition rate of the waste paper pulp is increased by 3.75 times and reaches 42.8%, and the white water is correspondingly increased by 38% and reaches 42.8%.

The invention belongs to the technical field of enzyme preparations and biological fermentation, and relates to a composite saccharifying enzyme for producing alcohol from thick mash corn and a use method of the composite saccharifying enzyme, and the composite saccharifying enzyme contains 70-90% of glucoamylase, 5-10% of pullulanase, 5-8% of acid protease and 3-5% of xylanase according to the enzyme activity unit ratio. The use method of the composite saccharifying enzyme in production of alcohol from thick mash corn comprises the following steps: S1, sieving corn flour, adding water, uniformly mixing, then adding alpha-amylase, heating, and liquefying to obtain liquefied mash; and S2, cooling the liquefied mash, adjusting the pH to be acidic, adding a compound saccharifying enzyme and saccharomyces cerevisiae, synchronously performing saccharification and fermentation treatment, and distilling after the treatment is completed to obtain the alcohol. The composite saccharifying enzyme disclosed by the invention can solve the problems of low starch utilization rate, high mash viscosity, more residual sugar and the like in the alcohol fermentation of the thick mash corn.

The invention relates to the technical field of dairy product processing, in particular to Chinese yam milk containing active protein and a preparation method thereof, and the Chinese yam milk comprises the following raw material components: raw milk, Chinese yam powder, gellan gum, sucrose fatty acid ester, glyceryl monostearate, glyceryl distearate, amylase, glucoamylase, food essence and water. According to the method, the Chinese yam and the milk are combined, a sweetening agent is not additionally added, meanwhile, the beverage is endowed with a sweet feeling, meanwhile, the damage of heat treatment to active protein in cow milk in the processing process is overcome, and the Chinese yam milk which is good in flavor and stable in system and contains the active protein is prepared.

The present invention provides a method for improving a defoaming effect of a fermentation liquid when lignocellulosic biomass is used. More specifically, the present invention provides a method for producing ethanol from lignocellulosic biomass, the method involving a multiple parallel fermentation step, a multiple semi-parallel fermentation step, or a sequential fermentation step, wherein when the multiple parallel fermentation step is involved, the multiple parallel fermentation step involves a step for adding a defoaming agent to a fermentation liquid containing lignocellulosic biomass, a diastatic enzyme, and yeast; when the multiple semi-parallel fermentation step or sequential fermentation step is involved, the multiple semi-parallel fermentation step or sequential fermentation step involves a step for adding a defoaming agent to a saccharified solution containing lignocellulosic biomass and a diastatic enzyme and / or to a fermentation liquid containing the saccharified solution and yeast; and the defoaming agent includes a nonionic surfactant.

The invention discloses a high-efficiency environment-friendly starch sugar production method, which comprises: (1) crushing sorghum starch, screening, adding into water, adjusting the pH value, adding alpha-amylase and ferulic acid, completely liquefying, adding saccharifying enzyme and potassium sorbate, and saccharifying; and (2) refining and concentrating the saccharified sorghum starch in the step (1), then drying high-concentration sorghum starch syrup to obtain sorghum starch sugar powder, and finally packaging to obtain a finished product.

The present application belongs to the field of bioengineering technology, and particularly relates to a kind of enzymatic broussonetia papyrifera powder and its preparation method and application.The present application uses Aspergillus niger WZ001 as a starting strain, constructs and selects a strain of high-yield Aspergillus niger tannase, and then uses the recombinant tannase obtained by fermentation to enzymatically hydrolyze tannin in broussonetia papyrifera powder, which can convert broussonetia papyrifera powder tannin into gallic acid, which can effectively improve the palatability of feed using broussonetia papyrifera powder as one of the raw materials.On this basis, since the Aspergillus niger host has a rich enzyme system, amylase, glucoamylase, protease, cellulase, etc., the Aspergillus niger tannase of the present application can play the role of a complex enzyme, so it can enzymatically hydrolyze broussonetia papyrifera powder more comprehensively, and the product obtained after enzymatic hydrolysis has a higher nutritional value, which reflects a high economic value and social benefit.In addition, the Aspergillus niger tannase constructed in the present application can meet the requirements of food-grade enzyme preparation, so the safety of the enzymatically hydrolyzed broussonetia papyrifera powder is guaranteed.

The invention discloses a method for performing enzymolysis on flavonoid glycoside by using industrial saccharifying enzyme, and belongs to the technical field of bioactive substance conversion. The method comprises the following steps: dissolving a flavonoid glycoside substrate by using a DMSO solution to obtain a dissolved solution; then adding the dissolving solution, amyloglucosidase and a citrate buffer solution into a pore plate, putting the pore plate into a microplate reader, and carrying out enzymolysis efficiency test; and after the reaction is finished, wrapping the pore plate with tinfoil, and putting the pore plate into a freezing layer to inactivate the enzyme. The amyloglucosidase provided by the invention has a wider substrate spectrum, can realize comprehensive conversion of glycoside components with different structures in various flavone-rich complex plant raw materials, and does not need to independently adapt enzymes for specific glycoside types or raw material types; meanwhile, the amyloglucosidase can be used for treating various types of plant raw materials by virtue of a single enzyme seed, so that the use types of enzyme preparations are reduced, and the process complexity and cost are reduced.

The application discloses a kind of enzyme processing combination liquid fermentation production brewing type tobacco flavor agent's method, comprising the following steps: S1, cut tobacco is mixed with sorghum powder, after adding distilled water obtains tobacco-sorghum liquid medium;S2, yeast strain is inoculated to YPD culture medium and is cultivated, obtains yeast bacterial liquid;And / or lactobacillus strain is inoculated to MRS culture medium and is cultivated, obtains lactobacillus bacterial liquid;S3, configuration enzyme preparation solution, enzyme includes one or more of amylase, flavourzyme and saccharifying enzyme;S4, enzyme preparation solution is added to tobacco-sorghum liquid culture medium;S5, the yeast bacterial liquid and / or lactobacillus bacterial liquid of S2 is inoculated to the culture medium of S4 and is cultivated, obtains fermentation liquid flavor medium;S6, fermentation liquid flavor medium is freeze-dried, obtains tobacco flavor agent.The brewing type flavor agent of the application is prepared based on microbial fermentation technology, process is simple, condition is mild, cost is low, green environmental protection, improve the economic benefit of low quality cigarette.

The application relates to a method for synergistically treating kitchen garbage and forestry waste, comprising: first pretreating the kitchen garbage to obtain kitchen garbage oil phase, kitchen garbage liquid phase and kitchen garbage solid phase; second pretreating the forestry waste to obtain forestry waste solid phase; mixing the forestry waste solid phase and part of the kitchen garbage liquid phase and performing high-temperature treatment to obtain mixed slurry; performing first saccharification treatment on the mixed slurry and cellulase to obtain saccharified mixed slurry; mixing the remaining kitchen garbage liquid phase and the kitchen garbage solid phase to obtain kitchen garbage mixed slurry; performing second saccharification treatment on the kitchen garbage mixed slurry and saccharifying enzyme to obtain saccharified kitchen slurry; mixing the saccharified mixed slurry and the saccharified kitchen slurry to obtain fermentation raw material; and performing biological fermentation treatment on the fermentation raw material to obtain ethanol product. The method synergistically drives waste conversion through saccharification and fermentation, and increases efficiency and production.

The application provides a preparation method of anti-aging wheat starch; high-temperature-resistant alpha-amylase, papain and saccharifying enzyme are used for enzymolysis of young leaves of highland barley; the product after the enzymolysis is subjected to first solid-liquid separation, and residues are collected; the residues are added with xylanase and cellulase for enzymolysis; the product is cooled to room temperature and subjected to second solid-liquid separation; polysaccharide in the liquid is precipitated by ethanol; the precipitate is redissolved, dialyzed, concentrated by rotary evaporation and vacuum freeze-dried to obtain polysaccharide; the polysaccharide is dissolved in water to obtain a uniform polysaccharide solution; wheat starch is added into the polysaccharide solution; the mixture is stirred and uniformly mixed to obtain a mixed system; the mixed system is heated and gelatinized; the product is obtained by cooling at room temperature and freeze-drying; and the anti-aging wheat starch prepared based on the polysaccharide obtained from the enzyme residue can be used for making various snacks, such as crystal moon cake crust, shrimp dumplings, powder cakes, rice rolls and the like, and can enhance the anti-aging function of food.

The invention relates to a method for preparing resistant dextrin by using an intermittent microwave technology, which comprises the following steps: (1) adding an acid solution into starch, sealing and uniformly stirring to obtain acidified starch; (2) carrying out intermittent microwave operation on the acidified starch according to different empty-duty ratios to prepare a resistant dextrin crude product; and (3) dissolving the crude product of the resistant dextrin, performing enzymolysis and centrifugation through thermostable alpha-amylase and saccharifying enzyme, performing alcohol precipitation with ethanol, cleaning, and drying to obtain the resistant dextrin. By adopting the intermittent microwave method, the energy loss is reduced, the yield and the purity of the resistant dextrin are improved, and the production cost is saved.

The application discloses a kind of green efficient extraction of highland barley beta-glucan method, comprising the following steps: highland barley whole grain grinding powder;With highland barley whole grain powder is prepared powder;Ethanol enzyme removal impurity: highland barley powder is extracted with 80% ethanol, filtration and drying;Hot water extraction: distilled water extraction, centrifugal supernatant is combined;Enzymatic hydrolysis impurity: supernatant is sequentially added in high temperature resistant alpha-amylase, saccharifying enzyme, trypsin and is subjected to enzymatic hydrolysis, enzyme inactivation, centrifugal, and concentrates supernatant;Crude extraction: after alcohol precipitation, centrifugal washing, water freeze-drying is obtained beta-glucan extract.The application mainly uses clean energy such as ethanol, water, does not use organic chemical substance;The method is simple in operation, equipment cost is lower, and it is relatively energy saving and emission reduction;Extraction efficiency is higher, easy to factory implementation.

The invention belongs to the technical field of food processing, and particularly provides a compound preparation for improving the rancidity of bamboo powder, which comprises a compound enzyme A and a compound enzyme B, the compound enzyme A comprises neutral protease, lipase, glucose oxidase and catalase; the compound enzyme B is prepared from medium-temperature alpha-amylase, saccharifying enzyme and pullulanase. According to the composite preparation for improving the rancidity of the bamboo powder, specific enzymes are combined, and an optimized step-by-step enzymolysis process is combined, so that oxidation products causing the rancidity in the bamboo powder can be effectively degraded, or the formation way of the oxidation products can be blocked, the bamboo flavor can be remarkably reduced or eliminated, and meanwhile, main nutritional ingredients and quality of the bamboo powder are kept.