93 results about "Puerarin" patented technology

The invention discloses a preparation method of a liver protection medicine composition containing dihydromyricetin, and relates to the technical field of medicine preparation.According to the method, dihydromyricetin, puerarin, pueraria flower extract, corn oligopeptide, pyrroloquinoline quinone disodium salt, curcumin and ganoderan are used as raw materials; dihydromyricetin and puerarin are stirred, homogenized and dried in a mixed solvent to prepare the liver protection medicine composition, wherein the mass ratio of dihydromyricetin to puerarin is 1: (0.5-2). A multi-target liver protection system is constructed through cooperation of multiple components, the problems that a traditional liver protection medicine is poor in component solubility, easy to degrade in activity and weak in synergistic effect are solved, the bioavailability and stability of the product are improved, the process is simple and controllable, the preparation method is suitable for industrial production and can be prepared into oral preparations such as tablets and oral liquid, and the preparation method is suitable for industrial production. The composition is suitable for protection of alcoholic and chemical liver injury.

The invention discloses puerarin hydrogel based on a natural eutectic solvent as well as a preparation method and application of the puerarin hydrogel, and belongs to the technical field of pharmaceutical preparations and transdermal drug delivery. The preparation method comprises the following steps: mixing a hydrogen bond acceptor and a hydrogen bond donor, heating and stirring to obtain a natural eutectic solvent; puerarin is mixed with the natural eutectic solvent, and a puerarin solution is obtained; mixing the puerarin solution with a polyvinyl alcohol aqueous solution to obtain a mixed solution; and pouring the mixed solution into a mold or spreading the mixed solution into a film, and then carrying out freeze-thaw cycle to obtain the puerarin hydrogel based on the natural deep-eutectic solvent. According to the invention, a natural eutectic solvent is introduced into a hydrogel system, so that a carrier system with'high solubilization, gel forming and controllable physicochemical properties' is obtained, and meanwhile, the bottleneck of low solubility and low permeability of puerarin is solved. The puerarin hydrogel based on the natural eutectic solvent can be used as a skin external preparation for local administration.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

The application discloses a baicalin ternary co-amorphous drug and a preparation method and application thereof, and the amorphous substance can effectively improve the solubility and dissolution of puerarin and baicalin. The application mixes metformin hydrochloride, puerarin and baicalin according to a molar ratio of 1:1:1, and obtains the baicalin ternary co-amorphous drug through a ball milling method or a solvent-assisted milling method, the preparation method is green and efficient, the co-amorphous substance has high light stability and thermal stability, the solubility is increased by 1.7 times compared with that of a puerarin raw drug crystal, the solubility is increased by 46.8 times compared with that of a baicalin raw drug crystal, and the solubility is reduced by 14.8 times compared with that of a metformin hydrochloride raw drug crystal. The baicalin ternary co-amorphous drug has certain application prospects in liver protection, blood sugar reduction, treatment of damp-heat type colitis and cervical cancer.

The invention discloses a prebiotic black coffee beverage capable of improving the intestinal health function and a preparation method and application thereof, and belongs to the technical field of functional food. The invention discloses a prebiotic black coffee beverage with a function of improving intestinal health. The prebiotic black coffee beverage is prepared from the following raw materials: water, a black coffee concentrated solution, resistant dextrin, polydextrose, fructo-oligosaccharide, puerarin, mogroside and xanthan gum. The prebiotic black coffee drink added with prebiotics, dietary fibers and medicinal and edible components has the functions of improving intestinal health, promoting excretion and relaxing bowels, and is suitable for intestinal health management of people who have high sedentariness and irregular work and rest.

The invention discloses a method for efficiently extracting active ingredients from radix puerariae, the active ingredients comprise 3 '-hydroxypuerarin, the extraction method comprises the following steps: S1, raw material pretreatment: selecting dried radix puerariae tuberous roots, crushing and sieving with a 40-60-mesh sieve, and the invention relates to the technical field of plant active ingredient extraction. According to the method for efficiently extracting the active ingredients from the radix puerariae, through the synergistic effect of composite solvent extraction and gradient purification, synchronous and efficient enrichment is achieved on trace 3 '-hydroxypuerarin and a compound A in the radix puerariae, the extraction rate is larger than or equal to 15%, the extraction rate of the compound A is larger than or equal to 85%, and the extraction rate is increased by three times or above compared with a traditional single solvent extraction method; the total purity of the active ingredients is greater than or equal to 95%, the problems of low extraction rate of the target ingredients and difficult separation of impurities in the traditional process are solved, and the pharmacological activity of the 3 '-hydroxy puerarin and the compound A are synergistically enhanced, so that the application requirements of high-purity plant source active ingredients are met, and a high-quality raw material is provided for subsequent development.

The invention discloses a method for extracting puerarin from kudzu vine root peel by using an ultrasonic-assisted ionic liquid solution. The method comprises the following steps: by taking waste kudzu vine root peel as a raw material, carrying out drying and crushing pretreatment, carrying out oscillation extraction under an ultrasonic condition by taking an ionic liquid aqueous solution as an extraction solvent, and adsorbing and purifying an extracting solution through an adsorbent to realize separation of puerarin and the ionic liquid. According to the method, the ionic liquid is used as a green solvent, mass transfer is enhanced by combining the ultrasonic cavitation effect, the extraction rate of puerarin is remarkably increased, and efficient recovery of the ionic liquid is achieved. The process is simple and convenient to operate, can be carried out at room temperature, and has outstanding environmental protection advantages. According to the method, the extraction rate of puerarin can reach 91.4% under optimal conditions, the recovery rate of ionic liquid can reach 99.9% through adsorption and purification, and the relative content of puerarin in desorption liquid is 92.9%.

The present invention relates to the technical field of liver-protecting formula compositions, and in particular, to a liver-protecting milk thistle formula composition, and a preparation method thereof. Technical problems: the liver-protecting milk thistle formula composition, and a preparation method thereof are intended to solve the technical problems that most of existing liver-protecting compositions in the prior art are designed for the general liver protection needs of the general population and cannot specifically treat liver damage caused by excessive alcohol intake and liver diseases such as cirrhosis, acute hepatitis, hepatitis, fatty liver, cholangitis, cholelithiasis, psoriasis, and hypercholesterolemia. Technical solution: a liver-protecting milk thistle formula composition, including the following components: silymarin, puerarin, artichoke extract, glutathione, dandelion extract, and GABA. The liver-protecting formula composition has a good therapeutic effect on liver damage caused by excessive alcohol intake, and liver diseases such as cirrhosis, acute hepatitis, hepatitis, fatty liver, cholangitis, cholelithiasis, psoriasis, and hypercholesterolemia.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention relates to a florfenicol preparation for reversing drug resistance, and every 100 kg of the florfenicol preparation is prepared from the following raw materials by weight: 30 to 50 kg of florfenicol, 5 to 10 kg of puerarin, 0.5 to 1 kg of magnesium stearate, and the balance of silicon dioxide. The florfenicol preparation is subjected to low-temperature crushing treatment, the crystal form of the medicine can be prevented from being converted, the dissolution rate of the medicine is prevented from being reduced, the florfenicol and the puerarin are reasonably proportioned according to the formula, the optimal reverse drug resistance effect is achieved, and meanwhile, the product process is particularly suitable for industrial production.

The invention discloses a hydrogel dressing with antibacterial property, antioxidant property and pH responsiveness as well as a preparation method and application of the hydrogel dressing, and belongs to the technical field of biomedical materials. An aldehyde group generated after ring opening of genipin as a chemical cross-linking agent and an amino group in a high-molecular polymer are subjected to a Schiff base reaction to realize chemical cross-linking, puerarin molecules are self-assembled through non-covalent bonds including hydrogen bonds and hydrophobic interaction, and a gel network is formed; and Ag-coated CR composite nanoparticles synthesized by a microalgae extract and silver nitrate are loaded. The multifunctional hydrogel material provided by the invention has antibacterial, antioxidant and pH-responsive properties, shows excellent antibacterial ability for common pathogenic bacteria such as escherichia coli, staphylococcus aureus and the like, and can effectively inhibit wound infection.

The invention provides application of lactobacillus brevicornu and a fermentation composition thereof in hangover alleviating and liver protection, and relates to the technical field of biological fermentation, the lactobacillus brevicornu is lactobacillus brevicornu, the strain number of the lactobacillus brevicornu is KMHD10-222, and the registration number of the lactobacillus brevicornu in the China General Microbiological Culture Collection Center is CGMCC No. 32358. Traditional Chinese medicine substrates can be fermented, and the hangover-alleviating and liver-protecting effects of fermentation products are enhanced. The content of rare ginsenoside, total polysaccharide, total flavone, total phenol, puerarin, daidzein and dihydromyricetin of the lactobacillus brevis KMHD10-222 fermentation substrate extracting solution is increased, the effects of promoting ethanol metabolism and improving the alcoholic liver disease can be improved, and the effects of dispelling the effects of alcohol and protecting the liver are achieved.

The present invention relates generally to methods and compositions useful for treating skin on a subject. In some embodiments, methods and compositions disclosed herein comprise topical or oral administration of nutraceutical and / or topical composition, wherein the composition comprises at least two of puerarin, collagen, and hyaluronic acid.

The invention discloses a preparation method of glucosyl puerarin, which belongs to the technical field of pharmaceutical chemicals, and comprises the following steps: S1, adding sugar for reaction, mixing puerarin with pure water, adjusting the pH value to 4.5-5.5, adding beta-cyclodextrin, heating to 45-55 DEG C, adding cyclodextrin glucosyltransferase for reaction for 16-22 hours, then adjusting the pH value to 0.1-0.8, adding activated carbon, filtering filtrate and staying overnight at low temperature; s2, desugaring reaction: adjusting the pH value of the filtrate to 3.8-4.5, heating to 55-65 DEG C, adding saccharifying enzyme and glucose oxidase to react for 25-40 minutes, adjusting the pH value to 0.5-1.2, and filtering; s3, column purification: enabling a reaction solution to pass through a mixed column containing sulfonated modified XAD-16 and amino modified HPD-720 resin, and performing graded elution to collect an ethanol eluent; s4, concentrating and drying to obtain a product. The problems that the water solubility of puerarin is poor, the purity of glucosyl puerarin is low, and impurities are difficult to remove can be solved.

The application provides a pueraria ferment as well as a preparation method and application thereof. The preparation method of the plant ferment comprises the following steps: step 1, crushing dry pueraria root powder, mixing with water to obtain a pueraria root powder mixture; step 2, mixing the pueraria root powder mixture with a compound enzyme preparation to perform enzymolysis, to obtain an enzymolysis liquid; step 3, adding a carbon source into the enzymolysis liquid, mixing, and then performing a two-stage fermentation process; the first-stage fermentation is aerobic fermentation by using a bacterial agent containing yeast and acetic acid bacteria, and the second-stage fermentation is anaerobic fermentation by using a bacterial agent containing lactobacillus; and step 4, filtering the fermented mixture to obtain a clear solution, and the pueraria ferment is obtained. The obtained pueraria ferment has high contents of active ingredients such as puerarin and glucuronic acid, has good antioxidant capacity, has a health care effect, and has a liver protection effect.

The utility model relates to the technical field of puerarin extraction, in particular to a built-in detachable fresh air exchange module of an air purification door. Comprising a door body, a door lock arranged in the door body, a hinge arranged on one side of the door body, an air outlet formed in the front wall of the door body, an air inlet formed in the back wall of the door body, a fan arranged in the door body and a purification assembly arranged in the door body and located at the top end of the fan. And the sound insulation layer is arranged in the door main body and sleeves the fan. According to the air purification device, the purification assembly is arranged, the fan conveys air to pass through the purification assembly, the contact surface of filtered air and washing and disinfecting liquid is increased through the porous structure of the purification layer, and formaldehyde benzene series in the air are effectively adsorbed, dissolved, filtered and disinfected; the porous sponge material of the sound insulation layer can be used for buffering the ventilation module in the door main body when the door main body is opened and closed, and noise generated when the ventilation module is used can be reduced.

The application discloses a content determination method of Xintong oral liquid, which adopts UPLC method to determine the contents of puerarin, daidzin, 2,3,5,4 ’ tetrahydroxystilbene-2-O-beta-D-glucoside, naringin and icariin in the preparation. The quality detection method is stable and reliable, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of the Xintong oral liquid, is favorable for stabilizing the product quality, ensures the safety and effectiveness of clinical medication, and thus better meets the needs of medical treatment and market.

The invention discloses an application of a compound composition containing astragaloside IV in preparation of a medicine for resisting plateau fatigue, and the compound composition containing astragaloside IV is prepared from the following raw materials in parts by weight: 500 to 2000 parts of astragaloside IV, 5000 to 20000 parts of ginsenoside Rg1, 2 to 5 parts of vitamin D3 and 30000 to 150000 parts of puerarin. Animal experiments prove that the compound composition can relieve plateau exercise fatigue, improve the hypoxia tolerance, exercise fatigue resistance and oxidative stress resistance of a body and improve the comprehensive exercise performance of the body. According to the invention, the defect of single-component efficacy is overcome, a compound preparation is designed in a targeted manner, an innovative and feasible compound combination is provided, and a new scheme is provided for promoting athletic performance under plateau hypoxia.

The application discloses a co-crystal of puerarin (English name: Puerarin) and valine (English name: Valine) combined by non-covalent bond, a preparation method and application thereof. 21 H 20 O9·C5H 10 NO2; a preparation method of the co-crystal of puerarin and valine; and application of the co-crystal of puerarin and valine in preparation of a drug with effects of preventing and treating cardiovascular diseases, antioxidation, anticancer, anti-hypertension, blood sugar reduction, anti-inflammation, antipyretic analgesia, anti-Alzheimer's disease and anti-osteoporosis.

The invention discloses a puerarin nanobelt with stable homologous polysaccharide and a preparation method of the puerarin nanobelt, and belongs to the technical field of medicine nanobelts. The puerarin nanobelt is prepared by taking puerarin and pueraria polysaccharide as raw materials through a medium grinding method. The preparation method comprises the following steps: weighing a puerarin raw material medicine and a puerarin polysaccharide raw material medicine; adding the kudzuvine root polysaccharide into water until the kudzuvine root polysaccharide is completely dissolved; the preparation method comprises the following steps: adding water into a penicillin bottle, adding puerarin, adding a stirrer and 0.4-0.6 mm zirconium oxide beads with the same volume as water, putting the penicillin bottle on a magnetic stirrer, grinding at a magnetic stirring speed of 800-1400 rpm for 2-12 hours, taking out, and filtering to remove the zirconium oxide beads, thereby obtaining the puerarin nanobelt. The preparation method has the advantages that the polysaccharide stabilizer with a natural source is adopted, has the characteristics of good biocompatibility, low toxicity, biodegradability and the like, and shows remarkable advantages in a nano delivery system; the accumulative dissolution amount is greatly increased, and the balance time is greatly shortened; the oral absorption of puerarin can be obviously improved.

The invention discloses health wine capable of delaying senescence as well as a preparation method and application of the health wine, and belongs to the technical field of health food. According to the health-preserving wine, mulberries and sea-buckthorn are used as monarch materials, rhizoma polygonati and radix puerariae are used as ministerial materials, lophatherum gracile, fructus lycii and Chinese yam are used as assistants and guides, and all the raw materials are medicinal and edible substances. According to the invention, through an optimized'warm soaking-cold soaking 'two-step extraction process, synergistic interaction is realized, and active ingredients such as anthocyanin, flavone, polysaccharide and puerarin are extracted to the greatest extent. The health-preserving wine disclosed by the invention not only has good antioxidant capacity, but also can be used for remarkably removing free radicals such as DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl, improving the activity of serum SOD (superoxide dismutase) and GSH-Px (glutathione The formation of advanced glycosylation end products can be effectively inhibited, and the obvious anti-aging and anti-saccharification dual effects are achieved. The health-preserving wine disclosed by the invention is simple in preparation process, mellow in taste and suitable for daily health-preserving drinking.

The invention discloses puerarin-polypeptide co-assembled nano hydrogel, and belongs to the technical field of biological medicines. In order to solve the problems that puerarin is poor in water solubility and stability and diabetic wounds are difficult to heal, the hydrogel comprises self-assembled polypeptide and puerarin with the mass ratio of 1: (0.5-4), the molecular formula of the self-assembled polypeptide is R-GDFDFDY, D represents amino acid in a D configuration, and R is a non-steroidal anti-inflammatory drug. The self-assembled polypeptide and the puerarin are co-assembled to form the nano hydrogel, so that the solubility and the stability of the puerarin are improved, and the nano hydrogel can be used for preparing the diabetes wound healing medicine.

The application discloses a composition for treating radiation enteropathy and application thereof, and belongs to the technical field of biological medicine, and solves the problem that there is no effective treatment drug for radiation enteropathy at present. The composition comprises puerarin, baicalein, berberine and glycyrrhetinic acid. The composition for treating radiation enteropathy can regulate Wnt / beta-catenin, NF-kappa B and Caspase-9 / 3 signal pathways and the like, promote the proliferation of intestinal cells after radiation damage, reduce the inflammation level of intestinal tissues after radiation, reduce the apoptosis of intestinal cells after radiation and the like, improve the radiation damage of intestinal epithelial cells, play an anti-radiation enteropathy role, and has a remarkable effect on treating radiation enteropathy.

The application discloses a method for simultaneously determining contents of shugan su, puerarin and salvianolic acid B in health-care food by two-dimensional liquid chromatography, which comprises the following steps: extracting a sample to be measured by a solvent to obtain an extract, detecting the extract by a two-dimensional high performance liquid chromatograph, on-line center cutting, and quantifying by an external standard method. The method separates shugan su, puerarin and salvianolic acid B in the sample based on the on-line center cutting two-dimensional liquid chromatography, solves the problem that impurity peaks of salvianolic acid B exist under different mobile phase systems for health-care food with complex components, improves the separation degree and accuracy of salvianolic acid B, and the two-dimensional detection is on-line synchronous, which greatly improves the detection efficiency.

The invention discloses a method for promoting coconut callus induction by using puerarin, and relates to the technical field of plant tissue culture. According to the method, tender inflorescences of coconut trees serve as tissue culture explants, a Y3 culture medium serves as a basic culture medium, 3 mg / L puerarin is added in the callus induction stage, it is found that the puerarin can partially replace 2, 4-D or dicamba, the use amount of the 2, 4-D or dicamba is reduced, the risk of subsequent regeneration malformation is reduced, the browning rate of the explants is reduced by 43%, the callus induction rate is increased by 16%, and the survival rate of the explants is increased. And the induction time is shortened by 30 days. Aiming at the problems of explant browning and low callus induction rate caused by high-concentration herbicide plant hormones (2, 4-D or dicamba), puerarin is added in the coconut tissue culture regeneration process, so that the dosage of 2, 4-D or dicamba is reduced, the explant browning rate is reduced, the callus induction rate is improved, the induction time is shortened, and the tissue culture regeneration method has the advantages that the tissue culture regeneration efficiency is improved, and the tissue culture regeneration cost is reduced. The method has good popularization and application prospects in the field of coconut tissue culture and rapid propagation.

The application discloses a kind of load live probiotic traditional Chinese medicine hydrogel's adhesion microsphere and its preparation method and application, the preparation method of the microsphere includes: sodium alginate, traditional Chinese medicine active ingredient (such as puerarin) and live probiotic (such as paracasei) are mixed, and single dispersion droplet is formed by microfluidic electric spraying technology, and hydrogel microsphere is obtained by ion crosslinking solidification;Again, surface self-polymerization modification is carried out in dopamine weak alkaline solution, and polydopamine adhesion layer is formed.The mild method condition of the application, simple process, the particle size of the microsphere prepared is uniform and controllable.The microsphere has excellent three-dimensional network structure, can effectively protect the activity and metabolic function of internal probiotic;The polydopamine layer on the surface gives it strong wet skin adhesion, can resist flushing, realize long-acting retention.The microsphere can effectively inhibit acne propionibacterium and reduce inflammation through the synergistic effect of probiotic metabolite lactic acid and traditional Chinese medicine active ingredient, and can be used for preparing medicine for treating acne.

The invention discloses a method for simultaneously determining seven mass markers (Q-Marker) in serum of traditional Chinese medicine particles for treating cervical pain, which is characterized in that the seven mass markers are magnoflorine, puerarin, tetrahydropalmatine, paeoniflorin, notoginsenoside R1, ligustilide and decuricin; the method comprises the following steps: adding a serum sample into acetonitrile and acetone, carrying out vortex and centrifugation, taking a supernatant, loading the supernatant into a Hybrid SPEcolumn, washing with water to remove salt and polar interferents, eluting a target analyte with acetonitrile, filtering the eluent through a microfiltration membrane, carrying out sample introduction determination, and carrying out quantitative analysis by adopting a high performance liquid chromatography-mass spectrometry method and an electrospray ion source. And quantitative analysis is carried out in a positive / negative ion multi-reaction monitoring detection mode. The method is efficient, sensitive and high in selectivity, and solves the problems of serious matrix interference, indefinite target components, difficulty in simultaneous analysis of multiple components and the like in the prior art.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The invention discloses a thin-layer chromatography detection method of a traditional Chinese medicine composition, and quality control and evaluation of a ginseng-radix puerariae kidney-tonifying preparation. The method comprises the following steps: by taking a mixed solution of trichloromethane, methanol and water in a volume ratio of 10: (2-4): (0.1-0.3) as a developing solvent, carrying out thin-layer chromatography development, color development and inspection on a traditional Chinese medicine composition containing radix puerariae, herba epimedii and radix pseudostellariae raw materials, and comparing the traditional Chinese medicine composition with three reference substances, namely puerarin, icariin and epimedin C, according to the method, the identification of the radix puerariae and the herba epimedii in the traditional Chinese medicine composition is realized, the separation and detection of three active ingredients of puerarin, icariin and epimedin C are realized, the thin-layer chromatography spots are clear, and the separation effect is good. According to the detection method, the accuracy and comprehensiveness of quality control of the traditional Chinese medicine composition containing the radix puerariae, the herba epimedii and the radix pseudostellariae are improved, the method has the advantages of being easy and convenient to operate, low in cost and high in specificity, and establishment and overall evaluation of quality standards of the traditional Chinese medicine composition are facilitated.

The invention relates to the technical field of compound extraction and separation, and discloses a method for efficiently separating and purifying puerarin, which comprises the following steps: raw material pretreatment: crushing a radix puerariae raw material, and adding an aqueous solution containing a biosurfactant and sodium citrate for infiltration; gradient extraction: first-step extraction: adding an ethanol solution containing a solubilizer into the infiltrated material, stirring and extracting; 2, extraction: adding an ethanol solution containing a solubilizer into the filter residues, and carrying out ultrasonic-assisted extraction; separating and purifying: combining extracting solutions, centrifuging and collecting supernate; post-treatment: adjusting the pH value, and eluting through a macroporous resin column; and decolorizing the eluent with active carbon. A biological surfactant and sodium citrate are added in a raw material pretreatment stage, and crushing and infiltration treatment are combined, so that the extraction efficiency of puerarin and the utilization rate of raw materials are remarkably improved; the use of the biosurfactant enhances the solubility and dispersibility of puerarin, reduces the extraction of non-target components, and further effectively improves the extraction purity and yield of puerarin.