53results about How to "Excessive secretion" patented technology

The invention relates to a method of treating diverse human disorders that may arise, in part, out of sleep hypoxia and oxygen deprivation occurring in the context of sleep apnea / hypopnea disturbances. The disorders that may be treated by the invention comprise gastroesophageal reflux disease (GERD), asthma-associated gastroesophageal reflux (GER), GER-associated asthma, asthma, cardiomyopathy, cardioarrhythmia, congestive heart failure, sudden infant death syndrome, and diverse neurologic conditions. The mode of treatment uses somatostatin receptor ligands (SstRLs), particularly somatostatin-receptor agonists. The invention concerns the method of treatment utilizing, and compositions comprising SstRLs and somatostatin receptor agonists, including agonists of the somatostatin receptor types 2 and 5, particularly, the type 2A receptor (SsR-2A), including octreotide and lanreotide.

The invention discloses a method for preparing essence of stem cell factor with an anti-aging effect. The method comprises the steps that step 1, human umbilical cord mesenchyma stem cells are prepared and cultured, stem cell culture supernatant is collected, concentrate is obtained after concentration is conducted, and the concentrate is further frozen and dried to prepare the lyophilized powderof the stem cell factor; step 2, the components of, by volume, 0.1-1% of hyaluronic acid, 1-10% of glycerol, 0.1-1% of collagen protein, 0.1-1% of tea polyphenols and the balance water are mixed to obtain solvent; step 3, the solvent is added into the lyophilized powder of the stem cell factor to be thoroughly dissolved to obtain the essence of the stem cell factor. The prepared essence of the stem cell factor comprises multiple kinds of cell active factor components such as epidermal growth factor, vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor and the like,the essence can nourish skin, promote skin repairing and regenerating, resist wrinkles, remove colored patches and the like, and the essence has better application value in the field of medical beauty.

The invention discloses an exosome preparation derived from a stem cell. The exosome preparation is an exosome secreted by a Fe3O4 superparamagnetic nanoparticle stimulating the stem cell. The invention further provides the application of the exosome preparation in preparing a pharmaceutic preparation for promoting mesenchymal stem cell osteogenic differentiation and the application of the exosomepreparation in preparing a biological agent for treating orthopedic disorders or bone repair materials. In consideration of the complicated pathophysiologic process of osteonecrosis and bone defects,the exosome preparation prepared by the exosome secreted by the Fe3O4 superparamagnetic nanoparticle stimulating the stem cell is adopted, intramuscular administration can be adopted at local bone defect parts, or local filling is performed after the exosome preparation is mixed with absorbable gel, the exosome preparation directly reaches the focus, the osteogenic activity of the mesenchymal stem cell is motivated, the deposition of osteoblasts is promoted, and the repair of the osteonecrosis or bone defects is promoted.

The invention discloses a method for culturing nucleated pearls, which comprises the following steps of: taking a non-nucleated pearl as a nucleus or grinding shells to form the nucleus; cushioning a cell sheet under the nucleus, and implanting at the position of the viscera or foot of a freshwater mussel or nacre; returning the freshwater mussel or nacre to water for culture; and opening the freshwater mussel or nacre to harvest the pearls formed around the nucleus. Because the foot of the freshwater mussel or nacre is positioned above the visceral sac thereof, has a larger space and secretes more nacrum than the visceral sac or a mantle tissue, the implanted nucleus is difficult to be spit out, the self diameter of the nucleus is increased, and the freshwater mussel or nacre secretes more nacrum after the nucleus is coated. The pearls have the advantages of large particles, good transparency and gloss, short growth period and high economic value.

The invention discloses a pearl cultivating method for nuclear pearl, a tool adopting the method and cultivated pearl. The pearl cultivating method for nuclear pearl comprises the following steps: nuclear-free pearl is used as a pearl nucleus or a shell is grinded to form the pearl nucleus; the pearl nucleus is arranged on a cell sheet and then the pearl nucleus with the cell sheet is implanted on viscera or pelecypodium of a mussel or a pearl shell with a tool; the mussel and the pearl shell is restored in water for culture; the mussel or the pearl shell are opened and a pearl formed around the pearl nucleus is harvested. As the pelecypodium of the mussel or the pearl shell is above a visceral sac and has large space compared with the visceral sac or a mantle, and the pelecypodium secretes more nacre, the pearl nucleus cannot be spit out easily if the pearl nucleus is implanted on the pelecypodium and the diameter of the pearl nucleus is expanded, moreover, the mussel or the pearl shell secretes more nacre after the pearl nucleus is coated. The pearl cultivating method ensures that the pearl nucleus can be inserted into the pelecypodium accurately, the cell sheet can wrap the pearl nucleus better and the pearl nucleus has no need of punching a hole. The pearl has large particle, good translucent luster, short growth cycle and high economic value.

The invention relates to a double-loop oscillation perfusion-type biological reaction system which is applied to co-culture of seed cells and biomaterials. The biological reaction system comprises a support perfusion culture device, a liquid storage bottle, a recovery bottle and peristaltic pumps, wherein the support perfusion culture device is internally provided with a plurality of supports for co-culture of seed cells and biomaterials; the liquid storage bottle is used for conveying a culture solution in the bottle to the supports; the recovery bottle is used for receiving the culture solution flowing from the support perfusion culture device through a liquid conveying pipe; the flowing direction of the liquid storage bottle and the flowing direction of the recovery bottle are controlled through respective three-way pipes, so that the liquid storage bottle and the recovery bottle are switched between a perfusion liquid-changing channel and a perfusion culture channel; the peristaltic pump is respectively arranged in the perfusion liquid-changing channel and the perfusion culture channel and used for controlling the flow velocity of a culture solution passing through the liquid conveying pipe. According to the double-loop oscillation perfusion-type biological reaction system, the combination between an oscillation method and the perfusion method is achieved, the seed cells inside the biomaterials are subjected to sufficient substance interchange and stress stimulation, the production efficiency of engineered tissues is improved and the requirements of large-scale classed culture can be met.

The invention belongs to the technical field of foods, in particular to a paste food for recuperating and treating chromic gastrosis and a preparation method thereof. Solid matters of the paste food comprise the following raw materials in part by weight: 2 to 15 parts of codonopsis pilosula, 1 to 15 parts of rhodiola rosea, 1 to 15 parts of coix seeds, 0.5 to 10 parts of semen hoveniae, 0.5 to 10 parts of seville sour orange, 0.5 to 10 parts of bulb of fritillary, 0.5 to 10 parts of asiatic pennywort herb, 0.5 to 10 parts of dandelion, 1 to 30 parts of cactus, 0.2 to 10 parts of seabuckthorn oil, 2 to 30 parts of whey protein powder, 2 to 40 parts of isomalto oligosaccharide, 2 to 40 parts of xylooligosaccharide, 5 to 60 parts of inulin, 1 to 20 parts of viburnum fruit juice and 2 to 30 parts of rice starch. The paste food meets the requirements of gastrosis patients on having more meals a day but less food at each, can prevent and treat gastralgia, gastrectasia and gastric acid, can relieve economic burden of the gastrosis patients, has rational formula combination, is not cold or dry, has mild medicinal property and the effects of nourishing and circulating and recuperating to treat the diseases, does not have a side effect and conforms to health-care culture of medicament and food homology of traditional Chinese medical science.

The invention relates to a plasmid structure for expressing CD19CAR and knocking down T cell surface PD-1 expression simultaneously. The plasmid structure comprises an H1 promoter, an anti-PD-1shRNA sequence started by the H1 promoter, an EF1alpha promoter and a three-generation CD19CAR sequence started by the EF1alpha promoter that are connected in series in order. The three-generation CD19CAR sequence consists of a CD19 single chain variable region, a human CD8alpha hinge region, a human CD28 transmembrane region and an intracellular region, a human 41BB intracellular region and a human CD3zeta intracellular region that are connected in series. The plasmid structure provided by the invention is transduced into T cells by a lentivirus, and the CART cell production process is significantlysimplified than using CRISPR / Cas9 for knockout of PD-1. Moreover, the CART cells show enhanced tumor cytotoxicity killing ability and increased persistent cell repair and cytokine secretion ability after antigenic stimulation in vitro.

The invention belongs to the field of biological treatment of tumors and relates to an immune cell culture method capable of simultaneously activating CD4 <+>and CD8<+>T cells. The immune cell culture method comprises the steps of placing single nuclear cells which are well separated in a culture solution containing rhIL-12 and IL-4 ligands and an IL-10 ligand, culturing for 8-12h, then adding levamisole, continuously culturing for 8-12h, adding Anti-CD3McAb and rhIL-2, continuously culturing for 2-3 days, and then alternately adding into the culture medium containing the rhIL-2 and the culture medium containing the Anti-CD3McAb and the rhIL-2, wherein the intermediate culture time is 2-3 days. The scheme for preparing a cell factor compound is as follows: collecting cell supernatant liquid within 3-14 days after the beginning of culture, performing low-temperature ultracentrifugation, performing ultrafiltration by an ultrafiltration centrifugal tube, and performing microfiltration by a microprous filtration membrane, wherein filtrate is a natural cell factor mixture. By adopting the culture method provided by the invention, sustained proliferation of cells can be kept for 30 days. NCM contains a variety of cell factors and can stimulate immune cells of a human body to play autoimmune anti-tumor activity.

The invention discloses a composite premix used for improving female rabbit reproductive performance. Every 1kg of the composite premix comprises 13.60-16.22g of a composite vitamin premix, 1.91-2.16g of copper, 4.27-4.44g of iron, 3.7-4.2g of organic zinc, 14.22-4.55g of manganese, 0.035-0.045g of calcium iodate, 0.0195-0.0225g of organic selenium, 96.53-102.44g of lysine, 98.5-102.44g of methionine, 48-52g of choline, 7.5-22.5g of allitricin, 296-304g of brown sugar, 0.4-0.8g of ethoxyquin, 4-6g of soybean oil, 160-206g of montmorillonite, and balance of a carrier. When applied, the composite premix can be used as a 1% female rabbit composite premix. According to the invention, milking rabbit reproductive performance is improved, non-pregnant period is shortened, and stereo pollution of culturing to the environment is reduced.

The invention belongs to the technical field of wine making and particularly relates to a preparation process for waxy wheat Daqu. The process comprises the following steps: moistening materials, crushing, mixing the materials, pressing finished koji, putting the finished koji in a koji room to culture bacteria, overturning the koji and storing the koji in a warehouse to obtain finished Daqu product. The process is characterized in that raw materials for preparing the Daqu comprise waxy wheat. Furthermore, the raw materials for preparing the Daqu comprise the following components by weight percentage: 25%-100% of the waxy wheat and 0%-75% of hard wheat. According to the preparation process for the waxy wheat Daqu, the starch decomposing capability, the enzyme activity, the saccharifying capability, the liquidation capability and the acidic proteinase activity of the waxy wheat Daqu are improved greatly and the quality of the Daqu is improved; the preparation process for the waxy wheat Daqu has a very good application prospect and can be applied to a wine brewing process, vinegar brewing process and a soy sauce brewing process; the waxy wheat Daqu can be used partially or completely.

The invention provides a traditional Chinese medicine composition capable of removing acne, which comprises the following components by weight: 10-15 parts of viola tricolor, 8-10 parts of red sage root, 7-9 parts of sophora flavescens and 3-5 parts of garden burnet. The composition effectively combines several components together, enables synergism, is capable of removing acne, and has the efficacy of treating diseases and preserving health.

The invention discloses an application of a mesenchymal stem cell paracrine factor in eye drops, relates to an application of biological stem cell technology field in ophthalmology, and particularly relates to an application of the mesenchymal stem cell paracrine factor in the preparation of eye drops for macular degeneration of retina. A mesenchymal stem cell source for obtaining the paracrine factor includes umbilical cord mesenchymal stem cells, bone marrow mesenchymal stem cells, umbilical cord blood mesenchymal stem cells, adipose mesenchymal stem cells, placental mesenchymal stem cells,amniotic membrane mesenchymal stem cells and other mesenchymal stem cells from various tissues of all mammals, especially humans. The components of the eye drops are all mesenchymal stem cell paracrine factors, and the protein is cryopreserved to maintain the protein activity to the greatest extent.

The invention provides a method for obtaining mesenchymal stem cells with multidirectional differentiation potential and immunomodulatory function from children prepuce culture, comprising the following steps of: cleaning prepuce cut by circumcision with sterile PBS, and putting into a culture dish; longitudinally slicing the prepuce to diffuse tissues, separating epidermis from dermis, and then cutting the dermis into small pieces to form a tissue homogenate; uniformly inoculating the tissue homogenate into a culture bottle, horizontally placing the culture bottle to enable tissue blocks to be uniformly distributed on the whole bottom surface as much as possible, and adding a culture medium for primary culture; and finally, subculturing to the third generation to establish a mesenchymal stem cell bank. The culture method is high in speed, high in yield and rich in raw material source, and the cultured mesenchymal stem cells are high in purity, have multidirectional differentiation potential and immunomodulatory functions, can express more immunosuppression related genes and chemotactic genes, and can be used for treating various sepsis (infectivity (bacteria and viruses) and immunity) caused by inflammatory reaction.

The invention discloses preparation and prevention and treatment application of mycoprotein in a bacillus subtilis fermentation product. The preservation number of a bacillus subtilis strain DR22 is CGMCC NO: 19784. The bacillus subtilis strain DR22 has the advantages of being high in growth speed, high in sporulation quantity, high in stress resistance, rich in active products, high in mycoprotein and the like. The method is characterized in that a bacillus subtilis solid fermentation technology and a mycoprotein preparation technology are adopted, and the preparation process comprises the following steps: (1) activating strains to obtain activated strains; (2) carrying out multiplication culture to prepare a seed solution of a bacterial solution; (3) carrying out fermentation culture toprepare a microbial inoculum; and (4) preparing mycoprotein, namely fermenting thalli, drying, crushing, sieving, leaching, precipitating, carrying out isoelectric point acid precipitation, centrifugally collecting precipitates, and drying to remove moisture and mycoprotein. The final control effect can reach 73.76%, the control effect is stable, and a good biological control choice is provided for controlling tobacco mosaic virus diseases.

The invention aims at providing a traditional Chinese medicine composition, namely a capsule for gastrointestinal ulcer capable of curing the gastric ulcer and duodenal ulcer once and for all. The capsule is prepared from the following raw medicinal materials in parts by weight: 0.2-0.5 parts of bezoar, 3-9 parts of pseudo-ginseng, 3-15 parts of bighead atractylodes rhizome, 3-10 parts of rhizoma bletillae, 6-10 parts of codonopsis pilosula, 5-15 parts of astragalus membranaceus and 3-10 parts of liquorice. The capsule has multi-target curative effects on clinical treatment of the gastric ulcer and the duodenal ulcer as follows: 1, excessive secretion of gastric acid is inhibited, so that secretion of the gastric acid is returned to normal; 2, helicobacter pylori is killed, wherein the helicobacter pylori is a main pathogenic factor of stomach infection and duodenal ulcer, and also a chief culprit causing gastric perforation and a gastric cancer; 3, stomachache, stomach distension, dilute bowels, inappetence and malaise are quickly relieved; 4, the gastric and intestinal mucosa damage is repaired, so that the gastric and intestinal mucosa ulcer surface heals; 5, gastric perforation and gastric cancer complications are prevented.

Not only acute or chronic interstitial pneumonia and plumonary fibrosis but also chronic obstructive plumonary diseases, such as plumonary emphysema and chronic bronchitis, can be effectively prevented or treated by inhalation administration to the lung tissue of 2-{N-[4-(4-chlorobenzenesulfonylamino)butyl]-N-{3-[(4-isopropyl 2-thiazolyl)methoxy]benzyl}}-sulfamoylbenzoic acid or a pharmacologically acceptable salt thereof.

The invention discloses foot powder. The foot powder is prepared from, by weight, 50-80 parts of a filling agent, 2-6 parts of oyster extract, 1-4 parts of rhizoma atractylodis extract, 0.5-3 parts of spina date seeds, 0.1-2 parts of matrine extract, 1-15 parts of deodorant, 3-7 parts of surfactant, 0.05-0.15 part of preservative, 0.1-0.7 part of menthol, 0.1-0.5 part of borneol and 0.1-0.6 part of dipotassium glycyrrhizinate. The foot powder does not contain chemical additives, perfume is not added, natural ingredients contained in the foot powder are added into the formula after being extracted, the content and the utilization rate of the effective ingredients are increased, introduction of other impurities is reduced, irritation of the foot powder is effectively reduced, and the foot powder has the remarkable sweat relieving and odor restraining effects.

The invention relates to the field of livestock breeding, in particular to a whole-process efficient culture method for early breeding of Tibetan sheep. The method includes the steps of feeding and management of ewes in the suckling period, feeding and management of lambs in the suckling period, weaning selecting and remaining of earlier mating female lambs, breeding of the earlier mating female lambs, timely mating, feeding and management of ewes in the gestation period, and collecting lambs from primiparity ewes. By means of the comprehensive technical measures of breeding the earlier matingfemale lambs in the suckling period, conducting early weaning, conducting grazing, supplementary feeding and dynamic nutrient supplying in different developmental stages, conducting timely mating, conducting feeding and management of the primiparity ewes in the gestation period, collecting and breeding the lambs and the like, the ewes can give birth to one more batch of lambs within the feeding age limit, the birth weight of the lambs is increased, the death rate of the lambs is reduced, the lamb survival rate of the primiparity ewes reaches 97%, the reproduction and utilization rate of the ewes is increased, the reproduction rate is greatly increased, the interval of reproduction generations is shortened, and the feeding benefits are increased.

The invention provides a method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and a beautifying preparation. The method comprises the following steps: carrying out hypoxic starvation culture on P1-generation MSCs; carrying out hypoxic starvation culture on P2-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P3 generation; carrying out hypoxic starvation culture on P3-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P4 generation; and carrying out hypoxic starvation culture on P4-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P5 generation. The culture method can significantly promote MSCs to secret more active factors, and can particularly increase the yield of the active factors after low-temperature induction treatment. Secondly, a recovered cell factor stock solution can improve the effects of the beautifying preparation after filtration by activated carbon.

The invention relates to the technical field of the livestock breeding and in particular to a breeding method for improving a survival rate of a calf. The method specifically comprises the following steps: 1) during one month before the mating, feeding traditional Chinese medicine mixed grains every day, wherein the feeding amount is 200-300 g for cow and 350-500 g for bull; 2) after the pregnancy, feeding biological fermentation daily grains every day; 3) after the birth of the calf, cleaning the whole body of the calf by using a wet towel after 30 min while the everyday feeding is finished, illuminating the calf after the cleaning, wherein the illuminating time is 2-3 h / day, feeding breast milk after 15 days, feeding the calf separated from the cow and feeding a milk replacer. The provided breeding method is simple and convenient, and is easy to operate and control. The survival rate of the calf is high, the disease resistance is strong, and the drug residue is not existent. The breeding method is capable of, through the rational management of the mating cattle and the pregnancy, improving the health rate of the newborn cattle, guaranteeing the physique and the disease resistance of the newborn cattle, and guaranteeing the survival rate. Compared with the traditional method, the survival rate of the calf of the breeding method is improved by about 35%.

The invention relates to the technical field of medical care equipment and articles, particularly to a bionic breast pump, which comprises a milk discharging assembly, wherein a shell is divided intoan upper cavity and a lower cavity, the upper cavity is communicated with the lower cavity, the inner cavity of the upper cavity is matched with the outer contour of a milk collecting cover, a first connecting shaft is fixed to the upper portion of the lower cavity of the shell and hinged to one end of a jaw block, a second connecting shaft is arranged at the other end of the jaw block, the two ends of a crankshaft are hinged to the shell respectively, a motor is fixed to the bottom of the shell, a first gear is arranged at the output end of the motor, and a second gear meshed with the first gear is fixedly connected to the crankshaft. According to the invention, the bionic breast pump reduces the discomfort of a puerpera in a milk discharging process, avoids the problems of nipple pain, ulceration, mammary areola appearance change and the like caused by long-term repeated use, and can make hypothalamus secrete more prolactin by stimulating nerve reflex so as to promote milk secretion.

The invention relates to a medicine composition for controlling poultry gizzard erosion diseases, comprising the following ingredients: rhizoma bletillae powder, modified nano montmorillonite, cimetidine, methionine, and pharmaceutic adjuvant. The medicine composition has the effects of protecting gastric mucosa, promoting the mucosa healing, prevent harmful substance from damaging mucosa, improving hepatic / renal function, cooling blood, stop bleeding, eliminating stasis, and engendering flesh, and provides a feasible approach for industrial production.

The invention discloses a culture device for adherent cells, a 2.5D beehive type culture system and method. The culture device comprises a culture jar, a TC bracket and TC culture plates, wherein the TC bracket is arranged in the culture jar and is provided with a plurality of fixing parts which are arranged at different heights, and the fixing parts are used for mounting a plurality of TC culture plates in a layered manner; and the two surfaces of each TC culture plate are cell culture surfaces, and the TC culture plates are detachably arranged on the fixing parts with different heights respectively to form multiple layers of cell culture surfaces. The 2.5D beehive type culture system comprises the culture device and a mixture of an adherent cell culture solution and a macromolecular solute. According to the method, the mixture is injected into the 2.5D beehive type culture system for culture. The culture device provided by the invention is favorable for increasing the culture area of unit volume; the provided 2.5D beehive type culture system can simulate a 3D culture environment; and the method provided by the invention can be used for promoting cells to secrete more extracellular matrix proteins.

The invention relates to the technical field of biotechnology and food processing, in particular to an apple-flavored beverage and a preparation method thereof. The method comprises the following steps: soaking apple peel pomace in a citric acid solution, preparing a culture medium, carrying out first-stage aspergillus niger fermentation, crushing aspergillus niger cells, performing homogenizing,and carrying out second-stage probiotic fermentation. In the prior art, apple peel pomace contains a large amount of cellulose, pectin and other macromolecular substances, is difficult to use, pollutes the environment if discarded, and is difficult to degrade by a single microorganism. The invention provides the preparation method of the apple-flavored beverage. The apple peel pomace is soaked ina citric acid solution, browning can be prevented, productive turnover is facilitated, aspergillus niger and probiotics are fermented in different stages, products in a first stage are utilized through two-stage fermentation, the finally prepared apple-flavored beverage has the advantages of being high in living cell number of the probiotics and the like, and waste utilization of the apple peel pomace is achieved.

The invention relates to a sakura-flavored fermented rice and a preparation method thereof. The preparation method comprises the following steps: S1, preparing yeast fermentation liquor: (1) adding crushed fresh oriental cherry leaves, cane sugar, cellulase and a corn extract into a sodium chloride aqueous solution, completely dissolving the cane sugar and the cellulase, and performing sealed pickling for 24-36 hours; (2) after pickling is finished, adding activated saccharomyces cerevisiae into the pickling mixture and uniformly stirring the mixture, and continuing fermentation for 5-9 hours at normal temperature; s2, mixing glutinous rice and rice bran, cooking, spreading and cooling to obtain cooled rice, uniformly mixing the cooled rice with the mixed koji, and performing diastatic fermentation; s3, squeezing and filtering the saccharified and fermented mixture, pumping the mixture into a fermentation tank, and adding the yeast fermentation liquor prepared in S1 and sterile water into the fermentation tank for continuous fermentation; and S4, filling to obtain a finished product. The brewed rice wine is rich in flower fragrance but not greasy, mellow in taste and good in color, luster and taste. The method overcomes the defect that sakura-flavored rice wine prepared by a traditional method is tasteless in flower fragrance or the food safety problem caused by the fact that rice wine contains a large amount of essence.

The invention discloses a preparation method of an embryonic stem cell culture extract for skin aging resistance, and relates to the technical field of cosmetology, the preparation method comprises the following steps: S1, inoculating embryonic stem cells to an amplification culture medium for culture, collecting embryonic stem cell culture supernatant, and extracting cell secretions in the culture supernatant; and S2, concentrating and filtering the extracted culture secretions to obtain the embryonic stem cell culture extract. According to the preparation method of the embryonic stem cell culture extract for skin aging resistance, human embryonic stem cells are preferably selected as a source of the culture extract. A culture supernatant of the embryonic stem cells is rich in active factor components such as epidermal growth factors, vascular endothelial growth factors and stem cell factors, and the factors can promote growth and proliferation of in-vivo stem cells and promote fibroblasts and the like in skin to secrete more collagen. The biological effects have incomparable advantages compared with traditional cosmetics, and skin aging can be remarkably relieved.

The invention discloses a method for promoting the growth of zymomonas mobilis by using a philodina culture solution. The method comprises the following steps of firstly, culturing philodina by using a culture solution with flour as bait; when the density of the philodina reaches 100 to 500ind / mL, filtering the philodina culture solution by using a 300-mesh nylon net, and removing philodina from the philodina culture solution; secondly, filtering and separating obtained filtrate by using an ultrafiltration membrane, and further removing fine particulate matters such as flour and the like in the filtrate, thus obtaining filtrate, namely the philodina culture solution. The consumption of the philodina culture solution, disclosed by the invention, serving as a zymomonas mobilis growth promoter is 0.05 to 1 percent. The method for promoting the growth of the zymomonas mobilis by using the philodina culture solution, disclosed by the invention, has the characteristics of ecology, convenience in use and remarkable effects.

The invention relates to a perfusion bioreactor system which comprises a circulating perfusion system, a constant temperature system, a gas exchange system and an on-line monitoring system, wherein the circulating perfusion system comprises a culture chamber, a liquid storage bottle and a peristaltic pump which are sequentially connected through a pipeline to form a perfusion culture loop; the constant temperature system comprises a constant temperature water bath device of which the inside is provided with a circuitous circulating pipeline, and is applicable to an electric heating and refrigerating control part; the gas exchange system is composed of a gas filter, a gas flowmeter, a high-pressure gas bottle, a pressure relief valve and a connecting pipeline; and the on-line monitoring system is composed of a monitoring probe, a monitor terminal, monitoring software, a data conversion chip and a mass transfer regulator. The perfusion bioreactor system provided by the invention can maximally simulate the in-vivo microenvironment in the tissue or organ in-vitro construction process, ensures sufficient substance exchange and normal physiological functions of seed cells in the biological material, enhance the yield of the seed cell / biological stent material, shorten the culture period and lower the culture cost.