53results about How to "Excessive secretion" patented technology

The invention relates to a method of treating diverse human disorders that may arise, in part, out of sleep hypoxia and oxygen deprivation occurring in the context of sleep apnea/hypopnea disturbances. The disorders that may be treated by the invention comprise gastroesophageal reflux disease (GERD), asthma-associated gastroesophageal reflux (GER), GER-associated asthma, asthma, cardiomyopathy, cardioarrhythmia, congestive heart failure, sudden infant death syndrome, and diverse neurologic conditions. The mode of treatment uses somatostatin receptor ligands (SstRLs), particularly somatostatin-receptor agonists. The invention concerns the method of treatment utilizing, and compositions comprising SstRLs and somatostatin receptor agonists, including agonists of the somatostatin receptor types 2 and 5, particularly, the type 2A receptor (SsR-2A), including octreotide and lanreotide.

The invention discloses a method for preparing essence of stem cell factor with an anti-aging effect. The method comprises the steps that step 1, human umbilical cord mesenchyma stem cells are prepared and cultured, stem cell culture supernatant is collected, concentrate is obtained after concentration is conducted, and the concentrate is further frozen and dried to prepare the lyophilized powderof the stem cell factor; step 2, the components of, by volume, 0.1-1% of hyaluronic acid, 1-10% of glycerol, 0.1-1% of collagen protein, 0.1-1% of tea polyphenols and the balance water are mixed to obtain solvent; step 3, the solvent is added into the lyophilized powder of the stem cell factor to be thoroughly dissolved to obtain the essence of the stem cell factor. The prepared essence of the stem cell factor comprises multiple kinds of cell active factor components such as epidermal growth factor, vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor and the like,the essence can nourish skin, promote skin repairing and regenerating, resist wrinkles, remove colored patches and the like, and the essence has better application value in the field of medical beauty.

The invention discloses an exosome preparation derived from a stem cell. The exosome preparation is an exosome secreted by a Fe3O4 superparamagnetic nanoparticle stimulating the stem cell. The invention further provides the application of the exosome preparation in preparing a pharmaceutic preparation for promoting mesenchymal stem cell osteogenic differentiation and the application of the exosomepreparation in preparing a biological agent for treating orthopedic disorders or bone repair materials. In consideration of the complicated pathophysiologic process of osteonecrosis and bone defects,the exosome preparation prepared by the exosome secreted by the Fe3O4 superparamagnetic nanoparticle stimulating the stem cell is adopted, intramuscular administration can be adopted at local bone defect parts, or local filling is performed after the exosome preparation is mixed with absorbable gel, the exosome preparation directly reaches the focus, the osteogenic activity of the mesenchymal stem cell is motivated, the deposition of osteoblasts is promoted, and the repair of the osteonecrosis or bone defects is promoted.

The invention discloses a method for culturing nucleated pearls, which comprises the following steps of: taking a non-nucleated pearl as a nucleus or grinding shells to form the nucleus; cushioning a cell sheet under the nucleus, and implanting at the position of the viscera or foot of a freshwater mussel or nacre; returning the freshwater mussel or nacre to water for culture; and opening the freshwater mussel or nacre to harvest the pearls formed around the nucleus. Because the foot of the freshwater mussel or nacre is positioned above the visceral sac thereof, has a larger space and secretes more nacrum than the visceral sac or a mantle tissue, the implanted nucleus is difficult to be spit out, the self diameter of the nucleus is increased, and the freshwater mussel or nacre secretes more nacrum after the nucleus is coated. The pearls have the advantages of large particles, good transparency and gloss, short growth period and high economic value.

The invention discloses a pearl cultivating method for nuclear pearl, a tool adopting the method and cultivated pearl. The pearl cultivating method for nuclear pearl comprises the following steps: nuclear-free pearl is used as a pearl nucleus or a shell is grinded to form the pearl nucleus; the pearl nucleus is arranged on a cell sheet and then the pearl nucleus with the cell sheet is implanted on viscera or pelecypodium of a mussel or a pearl shell with a tool; the mussel and the pearl shell is restored in water for culture; the mussel or the pearl shell are opened and a pearl formed around the pearl nucleus is harvested. As the pelecypodium of the mussel or the pearl shell is above a visceral sac and has large space compared with the visceral sac or a mantle, and the pelecypodium secretes more nacre, the pearl nucleus cannot be spit out easily if the pearl nucleus is implanted on the pelecypodium and the diameter of the pearl nucleus is expanded, moreover, the mussel or the pearl shell secretes more nacre after the pearl nucleus is coated. The pearl cultivating method ensures that the pearl nucleus can be inserted into the pelecypodium accurately, the cell sheet can wrap the pearl nucleus better and the pearl nucleus has no need of punching a hole. The pearl has large particle, good translucent luster, short growth cycle and high economic value.

The invention relates to a plasmid structure for expressing CD19CAR and knocking down T cell surface PD-1 expression simultaneously. The plasmid structure comprises an H1 promoter, an anti-PD-1shRNA sequence started by the H1 promoter, an EF1alpha promoter and a three-generation CD19CAR sequence started by the EF1alpha promoter that are connected in series in order. The three-generation CD19CAR sequence consists of a CD19 single chain variable region, a human CD8alpha hinge region, a human CD28 transmembrane region and an intracellular region, a human 41BB intracellular region and a human CD3zeta intracellular region that are connected in series. The plasmid structure provided by the invention is transduced into T cells by a lentivirus, and the CART cell production process is significantlysimplified than using CRISPR/Cas9 for knockout of PD-1. Moreover, the CART cells show enhanced tumor cytotoxicity killing ability and increased persistent cell repair and cytokine secretion ability after antigenic stimulation in vitro.

The invention belongs to the field of biological treatment of tumors and relates to an immune cell culture method capable of simultaneously activating CD4 <+>and CD8<+>T cells. The immune cell culture method comprises the steps of placing single nuclear cells which are well separated in a culture solution containing rhIL-12 and IL-4 ligands and an IL-10 ligand, culturing for 8-12h, then adding levamisole, continuously culturing for 8-12h, adding Anti-CD3McAb and rhIL-2, continuously culturing for 2-3 days, and then alternately adding into the culture medium containing the rhIL-2 and the culture medium containing the Anti-CD3McAb and the rhIL-2, wherein the intermediate culture time is 2-3 days. The scheme for preparing a cell factor compound is as follows: collecting cell supernatant liquid within 3-14 days after the beginning of culture, performing low-temperature ultracentrifugation, performing ultrafiltration by an ultrafiltration centrifugal tube, and performing microfiltration by a microprous filtration membrane, wherein filtrate is a natural cell factor mixture. By adopting the culture method provided by the invention, sustained proliferation of cells can be kept for 30 days. NCM contains a variety of cell factors and can stimulate immune cells of a human body to play autoimmune anti-tumor activity.

The invention discloses a composite premix used for improving female rabbit reproductive performance. Every 1kg of the composite premix comprises 13.60-16.22g of a composite vitamin premix, 1.91-2.16g of copper, 4.27-4.44g of iron, 3.7-4.2g of organic zinc, 14.22-4.55g of manganese, 0.035-0.045g of calcium iodate, 0.0195-0.0225g of organic selenium, 96.53-102.44g of lysine, 98.5-102.44g of methionine, 48-52g of choline, 7.5-22.5g of allitricin, 296-304g of brown sugar, 0.4-0.8g of ethoxyquin, 4-6g of soybean oil, 160-206g of montmorillonite, and balance of a carrier. When applied, the composite premix can be used as a 1% female rabbit composite premix. According to the invention, milking rabbit reproductive performance is improved, non-pregnant period is shortened, and stereo pollution of culturing to the environment is reduced.

The invention provides a traditional Chinese medicine composition capable of removing acne, which comprises the following components by weight: 10-15 parts of viola tricolor, 8-10 parts of red sage root, 7-9 parts of sophora flavescens and 3-5 parts of garden burnet. The composition effectively combines several components together, enables synergism, is capable of removing acne, and has the efficacy of treating diseases and preserving health.

The invention discloses an application of a mesenchymal stem cell paracrine factor in eye drops, relates to an application of biological stem cell technology field in ophthalmology, and particularly relates to an application of the mesenchymal stem cell paracrine factor in the preparation of eye drops for macular degeneration of retina. A mesenchymal stem cell source for obtaining the paracrine factor includes umbilical cord mesenchymal stem cells, bone marrow mesenchymal stem cells, umbilical cord blood mesenchymal stem cells, adipose mesenchymal stem cells, placental mesenchymal stem cells,amniotic membrane mesenchymal stem cells and other mesenchymal stem cells from various tissues of all mammals, especially humans. The components of the eye drops are all mesenchymal stem cell paracrine factors, and the protein is cryopreserved to maintain the protein activity to the greatest extent.

The invention provides a method for obtaining mesenchymal stem cells with multidirectional differentiation potential and immunomodulatory function from children prepuce culture, comprising the following steps of: cleaning prepuce cut by circumcision with sterile PBS, and putting into a culture dish; longitudinally slicing the prepuce to diffuse tissues, separating epidermis from dermis, and then cutting the dermis into small pieces to form a tissue homogenate; uniformly inoculating the tissue homogenate into a culture bottle, horizontally placing the culture bottle to enable tissue blocks to be uniformly distributed on the whole bottom surface as much as possible, and adding a culture medium for primary culture; and finally, subculturing to the third generation to establish a mesenchymal stem cell bank. The culture method is high in speed, high in yield and rich in raw material source, and the cultured mesenchymal stem cells are high in purity, have multidirectional differentiation potential and immunomodulatory functions, can express more immunosuppression related genes and chemotactic genes, and can be used for treating various sepsis (infectivity (bacteria and viruses) and immunity) caused by inflammatory reaction.

The invention discloses preparation and prevention and treatment application of mycoprotein in a bacillus subtilis fermentation product. The preservation number of a bacillus subtilis strain DR22 is CGMCC NO: 19784. The bacillus subtilis strain DR22 has the advantages of being high in growth speed, high in sporulation quantity, high in stress resistance, rich in active products, high in mycoprotein and the like. The method is characterized in that a bacillus subtilis solid fermentation technology and a mycoprotein preparation technology are adopted, and the preparation process comprises the following steps: (1) activating strains to obtain activated strains; (2) carrying out multiplication culture to prepare a seed solution of a bacterial solution; (3) carrying out fermentation culture toprepare a microbial inoculum; and (4) preparing mycoprotein, namely fermenting thalli, drying, crushing, sieving, leaching, precipitating, carrying out isoelectric point acid precipitation, centrifugally collecting precipitates, and drying to remove moisture and mycoprotein. The final control effect can reach 73.76%, the control effect is stable, and a good biological control choice is provided for controlling tobacco mosaic virus diseases.

The invention aims at providing a traditional Chinese medicine composition, namely a capsule for gastrointestinal ulcer capable of curing the gastric ulcer and duodenal ulcer once and for all. The capsule is prepared from the following raw medicinal materials in parts by weight: 0.2-0.5 parts of bezoar, 3-9 parts of pseudo-ginseng, 3-15 parts of bighead atractylodes rhizome, 3-10 parts of rhizoma bletillae, 6-10 parts of codonopsis pilosula, 5-15 parts of astragalus membranaceus and 3-10 parts of liquorice. The capsule has multi-target curative effects on clinical treatment of the gastric ulcer and the duodenal ulcer as follows: 1, excessive secretion of gastric acid is inhibited, so that secretion of the gastric acid is returned to normal; 2, helicobacter pylori is killed, wherein the helicobacter pylori is a main pathogenic factor of stomach infection and duodenal ulcer, and also a chief culprit causing gastric perforation and a gastric cancer; 3, stomachache, stomach distension, dilute bowels, inappetence and malaise are quickly relieved; 4, the gastric and intestinal mucosa damage is repaired, so that the gastric and intestinal mucosa ulcer surface heals; 5, gastric perforation and gastric cancer complications are prevented.

Not only acute or chronic interstitial pneumonia and plumonary fibrosis but also chronic obstructive plumonary diseases, such as plumonary emphysema and chronic bronchitis, can be effectively prevented or treated by inhalation administration to the lung tissue of 2-{N-[4-(4-chlorobenzenesulfonylamino)butyl]-N-{3-[(4-isopropyl 2-thiazolyl)methoxy]benzyl}}-sulfamoylbenzoic acid or a pharmacologically acceptable salt thereof.

The invention discloses foot powder. The foot powder is prepared from, by weight, 50-80 parts of a filling agent, 2-6 parts of oyster extract, 1-4 parts of rhizoma atractylodis extract, 0.5-3 parts of spina date seeds, 0.1-2 parts of matrine extract, 1-15 parts of deodorant, 3-7 parts of surfactant, 0.05-0.15 part of preservative, 0.1-0.7 part of menthol, 0.1-0.5 part of borneol and 0.1-0.6 part of dipotassium glycyrrhizinate. The foot powder does not contain chemical additives, perfume is not added, natural ingredients contained in the foot powder are added into the formula after being extracted, the content and the utilization rate of the effective ingredients are increased, introduction of other impurities is reduced, irritation of the foot powder is effectively reduced, and the foot powder has the remarkable sweat relieving and odor restraining effects.

The invention provides a method for producing active factors by using perinatal mesenchymal stem cells (MSCs) and a beautifying preparation. The method comprises the following steps: carrying out hypoxic starvation culture on P1-generation MSCs; carrying out hypoxic starvation culture on P2-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P3 generation; carrying out hypoxic starvation culture on P3-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P4 generation; and carrying out hypoxic starvation culture on P4-generation MSCs, and collecting a culture supernatant, and then using a complete culture medium to passage to a P5 generation. The culture method can significantly promote MSCs to secret more active factors, and can particularly increase the yield of the active factors after low-temperature induction treatment. Secondly, a recovered cell factor stock solution can improve the effects of the beautifying preparation after filtration by activated carbon.

The invention relates to a medicine composition for controlling poultry gizzard erosion diseases, comprising the following ingredients: rhizoma bletillae powder, modified nano montmorillonite, cimetidine, methionine, and pharmaceutic adjuvant. The medicine composition has the effects of protecting gastric mucosa, promoting the mucosa healing, prevent harmful substance from damaging mucosa, improving hepatic/renal function, cooling blood, stop bleeding, eliminating stasis, and engendering flesh, and provides a feasible approach for industrial production.

The invention discloses a culture device for adherent cells, a 2.5D beehive type culture system and method. The culture device comprises a culture jar, a TC bracket and TC culture plates, wherein the TC bracket is arranged in the culture jar and is provided with a plurality of fixing parts which are arranged at different heights, and the fixing parts are used for mounting a plurality of TC culture plates in a layered manner; and the two surfaces of each TC culture plate are cell culture surfaces, and the TC culture plates are detachably arranged on the fixing parts with different heights respectively to form multiple layers of cell culture surfaces. The 2.5D beehive type culture system comprises the culture device and a mixture of an adherent cell culture solution and a macromolecular solute. According to the method, the mixture is injected into the 2.5D beehive type culture system for culture. The culture device provided by the invention is favorable for increasing the culture area of unit volume; the provided 2.5D beehive type culture system can simulate a 3D culture environment; and the method provided by the invention can be used for promoting cells to secrete more extracellular matrix proteins.

The invention relates to the technical field of biotechnology and food processing, in particular to an apple-flavored beverage and a preparation method thereof. The method comprises the following steps: soaking apple peel pomace in a citric acid solution, preparing a culture medium, carrying out first-stage aspergillus niger fermentation, crushing aspergillus niger cells, performing homogenizing,and carrying out second-stage probiotic fermentation. In the prior art, apple peel pomace contains a large amount of cellulose, pectin and other macromolecular substances, is difficult to use, pollutes the environment if discarded, and is difficult to degrade by a single microorganism. The invention provides the preparation method of the apple-flavored beverage. The apple peel pomace is soaked ina citric acid solution, browning can be prevented, productive turnover is facilitated, aspergillus niger and probiotics are fermented in different stages, products in a first stage are utilized through two-stage fermentation, the finally prepared apple-flavored beverage has the advantages of being high in living cell number of the probiotics and the like, and waste utilization of the apple peel pomace is achieved.

The invention relates to a sakura-flavored fermented rice and a preparation method thereof. The preparation method comprises the following steps: S1, preparing yeast fermentation liquor: (1) adding crushed fresh oriental cherry leaves, cane sugar, cellulase and a corn extract into a sodium chloride aqueous solution, completely dissolving the cane sugar and the cellulase, and performing sealed pickling for 24-36 hours; (2) after pickling is finished, adding activated saccharomyces cerevisiae into the pickling mixture and uniformly stirring the mixture, and continuing fermentation for 5-9 hours at normal temperature; s2, mixing glutinous rice and rice bran, cooking, spreading and cooling to obtain cooled rice, uniformly mixing the cooled rice with the mixed koji, and performing diastatic fermentation; s3, squeezing and filtering the saccharified and fermented mixture, pumping the mixture into a fermentation tank, and adding the yeast fermentation liquor prepared in S1 and sterile water into the fermentation tank for continuous fermentation; and S4, filling to obtain a finished product. The brewed rice wine is rich in flower fragrance but not greasy, mellow in taste and good in color, luster and taste. The method overcomes the defect that sakura-flavored rice wine prepared by a traditional method is tasteless in flower fragrance or the food safety problem caused by the fact that rice wine contains a large amount of essence.

The invention discloses a method for promoting the growth of zymomonas mobilis by using a philodina culture solution. The method comprises the following steps of firstly, culturing philodina by using a culture solution with flour as bait; when the density of the philodina reaches 100 to 500ind/mL, filtering the philodina culture solution by using a 300-mesh nylon net, and removing philodina from the philodina culture solution; secondly, filtering and separating obtained filtrate by using an ultrafiltration membrane, and further removing fine particulate matters such as flour and the like in the filtrate, thus obtaining filtrate, namely the philodina culture solution. The consumption of the philodina culture solution, disclosed by the invention, serving as a zymomonas mobilis growth promoter is 0.05 to 1 percent. The method for promoting the growth of the zymomonas mobilis by using the philodina culture solution, disclosed by the invention, has the characteristics of ecology, convenience in use and remarkable effects.