59 results about "Tumor cytotoxicity" patented technology

The invention discloses truffle-tartary buckwheat health care wine and a preparation method thereof. The truffle-tartary buckwheat health care wine is composed of the following components in parts by mass: 3-8 parts of truffle, 3-8 parts of tartary buckwheat bran and 92-97 parts of tartary buckwheat distilled liquor. The preparation method comprises the following steps: at first, cleaning, primary steaming, grain cooling, grain sealing, secondary steaming, cooling, cultivating bacteria, allocating slot, fermenting and distilling to obtain the tartary buckwheat distilled liquor, then uniformly mixing the tartary buckwheat distilled liquor with truffle, ultrasonically extracting the mixture for 1 hour, sequentially filtering by using rough kieselguhr and fine kieselguhr to obtain the truffle-tartary buckwheat health care wine. The truffle-tartary buckwheat health care wine provided by the invention has quite high nutrition and health care values, and has the functions of supporting Yang, conditioning endocrine, preventing Alzheimer disease, atherosclerosis and tumor cytotoxicity, enhancing immunity and resisting aging and fatigue and the like. The truffle-tartary buckwheat health care wine has the advantages of being quick to become effective, obvious in effect, high in safety, and simple and convenient preparation method, etc.

The invention belongs to the field of medical biology engineering, and particularly relates to a method for effectively multiplying gamma delta T cells by stimulating peripheral blood in vitro and application of the method. The method comprises the step of using feeder cells, an OKT3 (ornithine ketoacid transaminase) antibody, interleukin-2 and zoledronic acid. The feeder cells are formed by specifically inserting CD64, CD86 and CD137L genes in a target site of a genome of the feeder cells. After the zoledronic acid and the nterleukin-2 are used for increasing the proportion of the gamma delta T cells of the peripheral blood, protein products of genes, the OKT3 antibody and the interleukin-2 act in a combined manner, and the gamma delta T cells can be stimulated so that a large amount of gamma delta T cells can be multiplied. The multiplied gamma delta T cells can be used for killing tumor cells which are pretreated by the zoledronic acid, or the tumor cells can be directly killed by modifying and expressing chimeric antigen receptors (CAR) via a genetic engineering means. The gamma delta T cells which are obtained by the method have complete anti-tumor cytotoxicity, and can kill solid tumor cells and non-solid tumor cells.

The invention discloses a preparation method of an effective part of dendrobium officinale. The method comprises steps as follows: firstly, extracting fat-soluble extracts represented by bibenzyl from the dendrobium officinale with a high-concentration alcohol-water system, refining the extracts with a macroporous resin method to obtain a total bibenzyl effective part with the bibenzyl content higher than 50%, and preparing dregs of the extracts into dendrobium officinale total polysaccharides with water extraction and alcohol precipitation methods; drying and evenly mixing the total bibenzyl effective part and the dendrobium officinale total polysaccharides obtained in two steps to obtain a dendrobium officinale total effective part extract. According to the method, the defect of the extremely low extraction rate of dendrobium officinale fat-soluble active ingredients represented by the bibenzyl with traditional methods such as a water decoction method, a tea making method and the like is overcome, the utilization efficiency of the effective part of the dendrobium officinale is improved substantially, pharmacological anti-oxidation animal experiments and anti-tumor cytotoxicity experiments prove that the activity of the effective part is significantly higher than that of traditional aqueous extracts, and the effective part has the characteristics of high extraction rate and high activity.