Preparation method of embryonic stem cell culture extract for skin aging resistance

An embryonic stem cell and cell culture technology, which is applied in the field of preparation of embryonic stem cell culture extracts for skin anti-aging, can solve the problems of poor individual targeting, serious product homogeneity, and single active ingredients, and achieve smooth and supple skin. , Promoting growth and proliferation, good lasting effect

Inactive Publication Date: 2021-08-03
杭州露源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the cosmetic preparations on the market have a single active ingredient, are not highly targeted to individuals, have poor product effects, and have serious product homogeneity.

Method used

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  • Preparation method of embryonic stem cell culture extract for skin aging resistance
  • Preparation method of embryonic stem cell culture extract for skin aging resistance
  • Preparation method of embryonic stem cell culture extract for skin aging resistance

Examples

Experimental program
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Embodiment 1

[0026] see figure 1 , the present invention provides a technical solution: a preparation method of an embryonic stem cell culture extract for skin anti-aging, comprising the following steps:

[0027] S1: Inoculate the embryonic stem cells into the expansion medium for culture, collect the culture supernatant of the embryonic stem cells, and extract the cell secretion in the culture supernatant;

[0028] S2: Concentrating and filtering the extracted culture secretion to obtain an embryonic stem cell culture extract.

[0029] Those skilled in the art can be understood as:

[0030] 1. Cell culture supernatant pretreatment: Take the embryonic stem cell culture supernatant (fresh or rewarmed at -20°C), centrifuge at 3000g for 15 minutes to remove dead cells and debris, and transfer the supernatant to a sterilized 1L blue cap bottle (do not pour too clean).

[0031] 2. Filter out large particles: use 0.45 μm and 0.22 μm aqueous membranes to filter the supernatant in step 1, and s...

Embodiment 2

[0044] see figure 2 , an application method of embryonic stem cell culture extract for skin anti-aging, comprising the following steps:

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Abstract

The invention discloses a preparation method of an embryonic stem cell culture extract for skin aging resistance, and relates to the technical field of cosmetology, the preparation method comprises the following steps: S1, inoculating embryonic stem cells to an amplification culture medium for culture, collecting embryonic stem cell culture supernatant, and extracting cell secretions in the culture supernatant; and S2, concentrating and filtering the extracted culture secretions to obtain the embryonic stem cell culture extract. According to the preparation method of the embryonic stem cell culture extract for skin aging resistance, human embryonic stem cells are preferably selected as a source of the culture extract. A culture supernatant of the embryonic stem cells is rich in active factor components such as epidermal growth factors, vascular endothelial growth factors and stem cell factors, and the factors can promote growth and proliferation of in-vivo stem cells and promote fibroblasts and the like in skin to secrete more collagen. The biological effects have incomparable advantages compared with traditional cosmetics, and skin aging can be remarkably relieved.

Description

technical field [0001] The invention relates to the field of cosmetic technology, in particular to a preparation method of an embryonic stem cell culture extract for skin anti-aging. Background technique [0002] Embryonic stem cells (ESCs) are a type of cells isolated from early embryos. They have the characteristics of unlimited proliferation, self-renewal and multi-directional differentiation in vitro. They can differentiate into any cells of the three germ layers and have the ability to develop into various tissues. The ability to participate in the formation of part of the organization. [0003] Embryonic stem cells will secrete a variety of factors during the culture process, mainly including factors related to pluripotency, transcription factors, mRNA and microRNA, etc. These factors can inhibit the aging process of cells, change the epigenetic characteristics of target cells, and reverse the effector cells. In the poorly differentiated state, it plays a similar role...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/545A61P17/00A61P17/18A61K8/99A61Q19/00A61Q19/02A61Q19/08
CPCA61K35/545A61K9/0014A61P17/00A61P17/18A61K8/99A61Q19/00A61Q19/02A61Q19/08
Inventor 沈自慧王雪
Owner 杭州露源生物科技有限公司
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