589 results about "Endothelium" patented technology

The invention relates to a spontaneous cerebral hemorrhage postoperative intermuscular venous thrombosis risk prediction system based on a molecular mechanism and multi-modal data and a use method thereof, image space features are extracted through a multi-modal data level CNN to be complementary with time sequence features captured and detected by Transform, and support is provided for blood coagulation-endothelium-fibrinolysis three-channel activation degree calculation; a knowledge graph alignment module of a pathology and data level maps the cross-modal features to a unified semantic space, and corrects data conflicts with pathology time sequence constraints; and channel-space-time triple attention linkage at a space-time level is carried out, and a high-risk area is accurately marked. In a path driving layer, three-path activation degrees cooperatively adjust a dynamic time window, a single-path standard-exceeding triggering monitoring interval is shortened, and a double-path standard-exceeding amplitude is multiplied; and in a closed-loop feedback layer, system parameters are updated through confidence, pathological probability and conflict signal recursion, real-time calibration of a pathological mechanism and data is realized, and thrombus risk prevention and control efficiency is comprehensively improved.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention belongs to the field of biological medicines, and finds and verifies that taquinimod or pharmaceutically acceptable salts thereof can be used for preparing medicines for treating endometriosis for the first time, and is particularly suitable for progestogen-resistant, recurrent or deep infiltration type endometriosis. The action mechanism of the taquinimod is as follows: the taquinimod can down-regulate the expression of S100A9 protein and reduce the infiltration of S100A9 + macrophages by inhibiting an NF-kappa B signal channel so as to further inhibit the expression of a vascular endothelial growth factor receptor 1 (VEGFR1) and a matrix metalloproteinase 2 (MMP2), finally block pathological angiogenesis and inhibit the growth of ectopic lesions. Animal experiments show that the taquinimod can significantly reduce the number and weight of nidus of a mouse model with endometriosis. The invention provides a brand-new non-hormone treatment choice with a clear action mechanism for clinical application, provides a pharmaceutical composition containing a specific dosage and a drug combination scheme, and has an important clinical value.

The invention relates to the technical field of biomedical materials, in particular to a double-network structure artificial blood vessel with anticoagulation, endothelium promotion and anti-infection functions and a preparation method of the double-network structure artificial blood vessel. The artificial blood vessel comprises a polymer fiber network artificial blood vessel substrate, a microscopic network hydrogel layer penetrating through the polymer fiber network artificial blood vessel substrate, and magnesium-copper alloy particles doped in the hydrogel layer. According to the artificial blood vessel with the double-network structure, the problem that a traditional small-caliber artificial blood vessel is insufficient in mechanical performance is greatly solved, copper ions and magnesium ions are released to the surrounding environment by means of degradation of the magnesium-copper alloy particles in the using process, the microenvironment of the surrounding tissue is adjusted to be alkalescent, nitric oxide is catalytically released, and the mechanical performance of the artificial blood vessel is improved. The artificial blood vessel with the double-network structure is endowed with anticoagulation, endothelium promotion and anti-infection functions. The problems of restenosis and insufficient endothelialization of a small-caliber artificial blood vessel in the prior art are comprehensively solved, and the long-term patency rate of the artificial blood vessel is increased.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

The invention relates to the field of medical instruments and public health, in particular to an artificial blood vessel with anticoagulation, endothelium promotion and bending resistance functions and a preparation method of the artificial blood vessel, and the artificial blood vessel is used for various blood vessel replacement and blood vessel bypass operations and has the characteristics of anticoagulation, endothelium promotion and bending resistance. The copper-containing material and the degradable magnesium-based metal material are respectively integrated on the inner surface of the artificial blood vessel and in the interlayer of the vessel wall, so that the functions of anticoagulation, endothelium promotion and bending resistance of the artificial blood vessel are realized. According to the artificial blood vessel with the anticoagulation, endothelium promotion and bending resistance functions, in the initial stage of use, due to the spiral structure of the magnesium alloy wire, the blood vessel cannot collapse after being bent, the blood flow is kept smooth, copper ions, magnesium ions and a small amount of hydrogen can be released to surrounding tissues in the use process, the microenvironment of the surrounding tissues is adjusted to be alkalescent, and the blood circulation is kept smooth. Through the synergistic effect of the copper ions and the magnesium alloy, the effects of promoting endothelialization, resisting blood coagulation and adjusting the acid-base property of the microenvironment are achieved at the same time, and therefore the functions of resisting blood coagulation, promoting endothelium and resisting bending are achieved.

Provided herein are methods of preparing an engineered heart valve for implantation into a subject in need (e.g., a pediatric patient). For example, a porcine pulmonary valve can be decellularized and then recellularized with cells from the subject. Hydrogels can be used to facilitate cell delivery and growth within the sub-endothelial layer (e.g., spongiosa) of a heart valve, which is essential for long-term success of the implantation.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The application provides an absorbable vascular stent covering facilitating endothelialization and a preparation method thereof, and belongs to the technical fields of biomedical materials and vascular stent covering preparation. The absorbable vascular stent covering facilitating endothelialization is prepared based on modified silk fibroin and domestic silkworm silk. The inner surface of the absorbable vascular stent covering facilitating endothelialization has a nano topological structure and a matrix-like component simulating a vascular basement membrane, and the outer surface has a micro topological structure. The absorbable vascular stent covering facilitating endothelialization has good mechanical properties and excellent endothelial cell activity, and can be used for producing a vascular stent capable of rapidly forming a new endothelial layer, maintaining stable blood flow, and inhibiting thrombosis and inflammation.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating gastroesophageal reflux cough and a preparation method thereof. The traditional Chinese medicine composition disclosed by the invention is constructed by carefully selecting a medicine formula under the guidance of a traditional Chinese medicine theory and clinical experience and through a compatibility theory of monarch, minister, assistant and guide of traditional Chinese medicines. In the traditional Chinese medicine composition, the inula flower has the effects of promoting blood circulation, removing stasis and turbidity, clearing the airway and removing phlegm and fluid retention, and the mangnolia officinalis has the effects of promoting qi circulation, removing food retention, calming adverse-rising energy and relieving asthma, and both serve as monarch drugs; the dried orange peel has the effects of regulating qi and removing stagnation, the poria cocos has the effects of tonifying spleen, eliminating dampness and phlegm and breaking up the source of phlegm, and the bulbus fritillariae cirrhosae has the effects of clearing heat and moistening lung and resolving phlegm and relieving asthma; the rhizoma atractylodis macrocephalae has the effects of invigorating the spleen, replenishing qi, eliminating dampness and inducing diuresis, and the endothelium corneum gigeriae galli has the effects of promoting digestion and invigorating the stomach and is an assistant and guide drug. The whole prescription is ordered in monarch and minister, focuses on pathogenesis, is rigorous in prescription, has the effects of relieving cough and asthma, clearing heat and moistening lung, and strengthening spleen and stomach, and treats both symptoms and root causes of cough.

This utility model relates to an innovation of a carotid artery vasodilator that can be used at home and in the emergency room as a supplement to drug treatment for conditions such as carotid artery obstruction in the neck region. It increases the production of nitric oxide mediated by endothelial nitric oxide synthase in the intact endothelial layer of the carotid artery, which is located on the inner surface of the neck brace. It can be applied for a certain period of time for each patient. The intermittent or continuous operation function can be selected through an adjustable power circuit. It only affects the lesion area and does not affect the patient's peripheral blood vessels. It can be used in situations where nitrates may be prohibited. It prevents the reduction of blood flow to the brain tissue that may occur due to jugular venous constriction. The flexible structure of the applicator neck brace (9) can be maintained in the application area for a long time and includes a pulsed magnetic field (PMF).

The invention relates to an endothelialization-promoting antifouling developing coating material as well as a preparation method and application thereof. The endothelialization-promoting antifouling developing coating material is obtained by reacting a siloxane-modified developing agent with a modified phosphorylcholine polymer; the modified phosphorylcholine polymer is obtained by copolymerization of a double-bond-containing monomer, and the double-bond-containing monomer comprises a combination of a double-bond-containing phosphorylcholine monomer, an endothelialization-promoting monomer and a siloxane monomer. According to the endothelialization-promoting antifouling developing coating material disclosed by the invention, a siloxane-modified developing agent is connected with a modified phosphorylcholine polymer. The siloxane-modified developing agent improves the developing function, and the modified phosphorylcholine polymer provides antifouling and endothelialization promoting functions, so that a composite coating with antifouling, developing and endothelialization promoting functions is formed.

The present disclosure provides a construct comprising a coding sequence operably linked to a promoter, wherein the coding sequence encodes a vascular endothelial growth factor (VEGF) binding agent or a portion thereof. In some embodiments, a construct is an AAV construct. In some embodiments, an AAV construct is a part of an AAV particle. Compositions comprising constructs and AAV particles described herein can be useful in treating hearing loss, for example, hearing loss associated with vestibular schwannoma.

The present application relates to the technical field of medical devices, and provides a system with a spiral structure for cavity embolization and a delivery method, the system comprising: an embolization device which is in a spiral structure in a natural state and can be freely stretched and contracted under stress; the embolization device comprises an inner mesh layer and an outer mesh layer which are concentrically arranged, and the sparseness of the outer mesh layer is greater than that of the inner mesh layer; the embolization device is used for sleeving the embolization stent and the inner circle of the spiral structure is tightly attached to the outer wall of the embolization stent. The system, after the release of the embolization device with a spiral structure, can tightly adhere to the periphery of the embolization stent, increase the diameter of the cavity part of the embolization stent, fill the entire cavity or cyst in a physical occupation manner, improve the occlusion effect, avoid the occurrence of internal leakage, and thus avoid secondary surgery. Moreover, since the sparseness of the outer mesh layer is greater than that of the inner mesh layer, the blood flow direction can be better changed, the blood flow speed is reduced, the endothelial material in the blood is quickly deposited in the embolization, the endothelialization is completed, and the tumor cyst is atrophied.

The present invention discloses a cardiovascular intervention device, which belongs to the technical field of cardiovascular surgical instruments. The puncture catheter is pre-filled with liquid medicine. As the air pump inputs gas, the mesh stent is stretched and the contracted blood vessel position is expanded. As the air pump continues to inflate, the piston head extends the pointed part from the inflation port of the puncture catheter, punctures the end of the airbag, and the liquid medicine inside the airbag flows out and diffuses at the vascular surgical site. This device can be used to quickly diffuse immediate-acting drugs such as blood vessel recovery and inhibition of endothelial hyperplasia at the surgical site while performing cardiovascular dilation surgery. Compared with applying the drug directly to the mesh stent, this device can input more liquid medicine at one time, and the amount of liquid medicine is controllable. When the liquid medicine reaches the surgical site, it will not be consumed in other positions of the blood vessel, which is more conducive to the treatment of the patient's vascular surgical site. This intervention device is simple to operate, does not affect the progress of the dilation surgery, and has a better treatment effect.

The invention relates to a crystal form of a compound shown as a formula I or a pharmaceutically acceptable salt thereof and a preparation method of the crystal form of the compound shown as the formula I or the pharmaceutically acceptable salt thereof. The compound of the formula I or the pharmaceutically acceptable salt thereof is used for treating cardiovascular diseases, endothelial dysfunction, diastolic dysfunction, atherosclerosis, hypertension, heart failure, pulmonary arterial hypertension (WHO levels I, II, III, IV), angina pectoris, thrombus, restenosis, myocardial infarction, stroke, cardiac insufficiency, fibrosis, pulmonary hyperplasia, erectile dysfunction, asthma, chronic kidney disease, diabetes, and the like. The composition can be used for treating liver cirrhosis, chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome, acute lung injury, pulmonary fibrosis, cystic fibrosis or interstitial lung disease.

The mouse intestinal tumor cell injector comprises a handle, an injection device installed in the handle, and a radially telescopic support ring structure connected with the handle, tumor cells can be discharged from a first injection needle by operating a first push rod, and antibiotics can be discharged from a second injection needle by operating a second push rod; when the support ring structure is in a contracted state, the first injection needle and the second injection needle are located in the support ring structure; when the support ring structure is in an expanded support state, the needle heads of the first injection needle and the second injection needle can both extend to the outside of the outer edge of the support ring structure and are inserted into the mouse intestinal wall. The present application supports the mouse intestine by the support ring structure, supports the insertion of the first injection needle and the second injection needle into the mouse intestinal wall, ensures that only the mouse intestinal endothelium is punctured and the mouse intestinal canal is not punctured, ensures that tumor cells are injected into the mouse intestinal wall, and when the tumor cells are injected, antibiotics are also injected for anti-inflammatory treatment, which is beneficial to the recovery of the mouse intestinal puncture point.

The invention relates to a medical device for treating aneurysms, in particular a stent, including a compressible and expandable grid structure made up of grid elements. The grid structure has at least one closed cell ring which includes at most 12, in particular at most 10, in particular at most 8, in particular at most 6 cells directly adjacent to one another in a circumferential direction of the grid structure. The grid structure is provided at least in certain portions with a covering made of an electrospun fabric which has pores of irregular sizes. The covering includes over a surface area of 100 000 μm2 at least 10 pores which have a size of at least 15 μm2. The covering has a biocompatible, in particular antithrombogenic and / or endothelialization-promoting coating.

The present invention is concerned with a protein oligomer comprising (i) at least two NC-1 monomers of collagen 18 or (ii) at least two endostatin domains of collagen 18 or (iii) at least two N-terminal peptides of the collagen 18 endostatin domain, for use in treating, ameliorating or preventing fibrosis or a fibrosis-associated disease, a vascular endothelial growth factor (VEGF)-related disease or a matrix metalloproteinase (MMP)-related disease. The invention further relates to the mentioned protein oligomer for use for detecting and / or diagnosing fibrosis or a fibrosis-associated disease, a vascular endothelial growth factor (VEGF)-related disease or a matrix metalloproteinase (MMP)-related disease.

The invention provides application of taurochenodeoxycholic acid in preparation of a medicine for preventing and / or treating Fuchs corneal endothelial dystrophy, and particularly belongs to the technical field of medicine preparation. The invention provides an application of taurochenodeoxycholic acid in preparation of a medicine for preventing and / or treating Fuchs corneal endothelial dystrophy. Test results show that taurochenodeoxycholic acid can realize prevention and / or treatment of corneal endothelial cell injury caused by UVA; the number reduction, activity reduction, oxidative stress, ROS level rise and mitochondrial membrane potential drop of corneal endothelial cells caused by UVA are relieved; mitochondrial biological energy deficiency caused by UVA is relieved; and the prevention and / or treatment of the Fuchs corneal endothelial dystrophy are / is realized by activating a Ca < 2 + > signal channel.

The application discloses an AMD efficacy prediction method, system, device and medium based on feature fusion, belongs to the technical field of efficacy prediction, and comprises the following steps: acquiring optical coherence tomography image data and demographic characteristics of an age-related macular degeneration patient; determining deep learning features and imaging features according to the optical coherence tomography image data; performing feature selection based on the deep learning features, the imaging features and the demographic characteristics to obtain input features; performing calculation by combining a time series analysis network with the input features to determine a hidden state; and performing feature change trend analysis according to the hidden state to determine an age-related macular degeneration efficacy prediction result. The application proposes a multi-feature fusion long short-term memory network, and realizes efficacy prediction of anti-vascular endothelial growth factor treatment for the age-related macular degeneration patient.

The present invention is related to a drug-coated covered stent the drug coated covered stent is a novel medical device designed to address Type III coronary artery perforations during percutaneous coronary intervention (PCI). This system combines a balloon-expandable stent covered with a biocompatible graft material and coated with cytotoxic drugs at its ends to prevent excess tissue growth and promote endothelialization. through a rapid exchange delivery system, the DCCS can be precisely positioned and expanded within the artery, sealing tears and promoting healing. The device's ultrathin profile and high flexibility enable navigation through tortuous vessels. Key components are a blocking balloon (201), second guidewire (203), covered stent (205), proximal and distal shafts of the delivery system (1, 2, 3, 15 4), stent (5), graft material (6), cytotoxic drug coatings (8, 9), and endothelialization (11). This innovative solution offers a promising approach to managing coronary artery perforations, potentially reducing the need for surgical interventions.

The present invention relates to a polypeptide configured to assemble into an inhibitor of vascular endothelial growth factor (VEGF), a protein comprising said polypeptide, a nucleic acid molecule encoding said polypeptide or protein, an expression vector comprising the nucleic acid molecule, a recombinant host cell comprising said polypeptide, protein, nucleic acid molecule and / or expression vector, a pharmaceutical composition comprising the said polypeptide, protein, nucleic acid molecule, expression vector and / or host cell, and to a kit.

This invention provides a near-infrared therapy device for improving Alzheimer's disease, characterized by comprising: a frequency-modulated drive circuit 1, a near-infrared LD light source 2, an optical fiber coupler 3, a light source power monitoring photodetector (PD) 4, an optical power monitoring circuit 5, an optical fiber beam splitter 6, an MPO optical fiber cable 7, and an optical transmitter 8. The optical transmitter includes an optical transmitter housing 8-1, a fiber-end hemispherical emitting microlens 8-2, and a beam-shaping coupling lens 8-3. This invention can be used to regulate the metabolism and cellular function of meningeal lymphatic endothelial mitochondrial cells, promote meningeal lymphatic transport in the central nervous system, thereby alleviating the pathological symptoms of Alzheimer's patients and enhancing their cognitive function. The device has a simple and compact structure, is easy to plug and unplug, has low optical signal loss, and high coupling efficiency. It can be widely used in novel non-invasive optical brain-computer interface devices and belongs to the field of fiber optic sensing technology.

The invention relates to the technical field of biological medicines, in particular to a preparation method of a stem cell factor composition for promoting scalp hair follicle regeneration. The method comprises the following steps: performing high-low oxygen cycle pre-stimulation and stepped hypoxia induction on umbilical cord mesenchymal stem cells, monitoring secretion kinetics of vascular endothelial growth factors (VEGF), hepatocyte growth factors (HGF), keratinocyte growth factors (KGF) and fibroblast growth factors 2 (FGF2) in real time, and determining the optimal collection time; performing ultrafiltration on the supernate to obtain a primary concentrated solution, optimizing the molecular weight cutoff based on the bioactivity retention rate after freezing and thawing, and concentrating to obtain a stem cell factor concentrated solution; the stem cell factor composition is obtained by combining the stem cell factor with a compound nutrition preparation, verifying in-vitro hair follicle cell proliferation / migration activity, dynamically adjusting the nutrition proportion according to the relative efficiency ratio and optimizing. According to the invention, intelligent preparation of the stem cell factor composition with multi-factor synergistic secretion, controllable process, stable activity and adjustable functions is realized.

Provided herein are compositions including a CRISPR / dCas9-based demethylation cocktail targeting a methylated endothelial PLCγ2 promoter and methods of treating wounds using the same.

The invention provides a blood concentration prediction model based on artificial intelligence and a construction method and application thereof.A whole blood sample of a patient taking sirolimus is collected, the blood concentration is detected through liquid chromatography tandem mass spectrometry, CYP 3A4 / 5, CYP 2C8 and P-gp gene polymorphism is detected in combination with a high-resolution dissolution curve technology, and the blood concentration prediction model based on artificial intelligence is obtained. And constructing a database containing the multi-dimensional data. And training the database by using an extreme gradient lifting algorithm, establishing a high-precision sirolimus blood concentration artificial intelligence prediction model, and developing a mobile equipment end application to realize remote data acquisition and result display. The model is especially suitable for children with Carboxi-like vascular endothelioma, can dynamically monitor the blood concentration in real time, provides refined guidance for clinical treatment, reduces the treatment cost, and improves the treatment effect and safety.

To provide a deep neural network framework for processing OCT images to predict treatment intensity.SOLUTION: Systems and methods relate to processing optical tomography coherence (OCT) images to predict characteristics of treatment to be administered to effectively treat age-related macular degeneration. The processing may include pre-processing the image by flattening and / or cropping the image (230), and processing the pre-processed image using a neural network (235). The neural network may include a deep convolutional neural network. An output of the neural network can indicate a predicted frequency and / or interval at which treatment (e.g., anti-vascular endothelial growth factor therapy) is to be administered so as to prevent leakage of vasculature in the eye (240).SELECTED DRAWING: Figure 2