836 results about "Endothelium" patented technology

The invention relates to anti-drug neutralizing antibody analysis. In particular, the present application relates to methods for detecting neutralizing antibodies against a vascular endothelial growth factor (VEGF) binding moiety and a programmed cell death ligand-1 (PD-L1) binding moiety in a biological sample from an individual administered a bispecific antibody drug comprising the VEGF binding moiety.

The invention discloses a vascular endothelial growth factor (VEGF) and antibacterial peptide (AMP) loaded chitosan methacrylic acid (CSMA) hydrogel microneedle patch as well as a preparation method and application thereof, the microneedle patch takes CSMA hydrogel as a main body material, the CSMA hydrogel is crosslinked and cured through ultraviolet light, and the microneedle patch has the characteristics of simplicity and convenience in preparation and stable material performance. The microneedle patch can continuously and stably release VEGF and AMP, promote wound healing and inhibit bacterial infection. Experiments show that the hydrogel microneedle is non-toxic to cells, has good biocompatibility and biodegradability, and also has excellent antibacterial performance. The invention provides an efficient and safe novel treatment tool for repairing infectious wounds.

The invention relates to a spontaneous cerebral hemorrhage postoperative intermuscular venous thrombosis risk prediction system based on a molecular mechanism and multi-modal data and a use method thereof, image space features are extracted through a multi-modal data level CNN to be complementary with time sequence features captured and detected by Transform, and support is provided for blood coagulation-endothelium-fibrinolysis three-channel activation degree calculation; a knowledge graph alignment module of a pathology and data level maps the cross-modal features to a unified semantic space, and corrects data conflicts with pathology time sequence constraints; and channel-space-time triple attention linkage at a space-time level is carried out, and a high-risk area is accurately marked. In a path driving layer, three-path activation degrees cooperatively adjust a dynamic time window, a single-path standard-exceeding triggering monitoring interval is shortened, and a double-path standard-exceeding amplitude is multiplied; and in a closed-loop feedback layer, system parameters are updated through confidence, pathological probability and conflict signal recursion, real-time calibration of a pathological mechanism and data is realized, and thrombus risk prevention and control efficiency is comprehensively improved.

The invention relates to medical recombinant collagen repair gel and a preparation method thereof, and belongs to the technical field of recombinant collagen. Through the synergistic effect of the collagen, the chitosan, the polylactic acid, the nanocellulose, the vascular endothelial growth factor, the anti-inflammatory drug and the cell adhesion peptide, the tissue repair effect is remarkably improved. Through a gas foaming method, staged freezing and freeze drying, multistage pore size distribution is generated, gel with a porous structure is formed, and cell permeation and tissue regeneration are facilitated; in addition, the permeability and the drug loading capacity of the gel are improved, the cell adhesion peptides, the growth factors and the anti-inflammatory drugs are better adsorbed to the gel, functional slow release is achieved, and the repairing effect is further improved.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention provides a construction method and application of a blood sinus networked liver organ. According to the method, pluripotent stem cells are co-differentiated into HAND1 < + > mesoderm and endoderm, a front intestine rear endoderm containing KDR < + > mesoderm progenitor cells is generated, a liver endoderm with CD34 < + > / CD31 < + > endothelial progenitor cells is generated, and the blood sinus networked liver organ is formed. The liver organ has a functional blood sinus endothelial network penetrating through the liver cluster, and has the characteristic functions of low-density lipoprotein uptake, human serum albumin removal, mediated secretion of blood coagulation factor 8 and the like. The method provides a new-generation highly bionic research tool for liver disease mechanism analysis, drug screening and regeneration repair.

The invention relates to the technical field of biomedical engineering, and discloses a preparation method of a nano-enzyme SERS (Surface Enhanced Raman Scattering) sensor for detecting diabetic retinopathy, which comprises the following steps: (1) synthesizing a bimetallic gold-palladium nanorod; (2) assembling a gold trioctahedron array; and (3) modifying the surface of the gold-palladium nanorod synthesized in the step (1) with single-stranded DNA1 to prepare a nano-enzyme probe, hybridizing an aptamer of a vascular endothelial growth factor (VEGF) with single-stranded DNA2, modifying the surface of the gold trioctahedron array assembled in the step (2) with the hybridized aptamer, preparing a capture substrate, and constructing the nano-enzyme SERS sensor by the nano-enzyme probe and the capture substrate. In the detection process, the VEGF triggers a competitive recognition mechanism to promote the nano-enzyme probe to be captured by the capture substrate and catalyze tetramethyl benzidine (TMB) to generate oxTMB with a strong SERS signal. The sensor has the advantages of high sensitivity, strong specificity and good accuracy, and can realize rapid quantitative detection of DR biomarkers in serum.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention belongs to the field of biological medicines, and finds and verifies that taquinimod or pharmaceutically acceptable salts thereof can be used for preparing medicines for treating endometriosis for the first time, and is particularly suitable for progestogen-resistant, recurrent or deep infiltration type endometriosis. The action mechanism of the taquinimod is as follows: the taquinimod can down-regulate the expression of S100A9 protein and reduce the infiltration of S100A9 + macrophages by inhibiting an NF-kappa B signal channel so as to further inhibit the expression of a vascular endothelial growth factor receptor 1 (VEGFR1) and a matrix metalloproteinase 2 (MMP2), finally block pathological angiogenesis and inhibit the growth of ectopic lesions. Animal experiments show that the taquinimod can significantly reduce the number and weight of nidus of a mouse model with endometriosis. The invention provides a brand-new non-hormone treatment choice with a clear action mechanism for clinical application, provides a pharmaceutical composition containing a specific dosage and a drug combination scheme, and has an important clinical value.

The invention belongs to the technical field of cell mechanics biology experiment devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. The biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected, a micro-fluidic endothelial cell culture cavity and a peripheral system are constructed, real-time accurate control over aortic valve blood flow is achieved through the PID control system, and the system is suitable for large-scale popularization and application. Shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

Systems and methods relate to processing optical tomography coherence (OCT) images to predict characteristics of a treatment to be administered to effectively treat age-related macular degeneration. The processing can include pre-processing the image by flattening and / or cropping the image and processing the pre-processed image using a neural network. The neural network can include a deep convolutional neural network. An output of the neural network can indicate a predicted frequency and / or interval at which a treatment (e.g., anti-vascular endothelial growth factor therapy) is to be administered so as to prevent leakage of vasculature in the eye.

The present disclosure relates to fusion molecules and chimeric molecules which comprise two components: an Ang-2 antagonist peptide linked to a VEGF-binding moiety. Further disclosed are methods of using said chimeric molecules to treat a patient cancer, proliferative retinopathy, neovascular glaucoma, macular edema, wet age-related macular degeneration (wAMD), macular edema following retinal vein occlusion (RVO), diabetic macular edema (DME), or diabetic retinopathy (DR).

The invention relates to the technical field of biomedical materials, in particular to a double-network structure artificial blood vessel with anticoagulation, endothelium promotion and anti-infection functions and a preparation method of the double-network structure artificial blood vessel. The artificial blood vessel comprises a polymer fiber network artificial blood vessel substrate, a microscopic network hydrogel layer penetrating through the polymer fiber network artificial blood vessel substrate, and magnesium-copper alloy particles doped in the hydrogel layer. According to the artificial blood vessel with the double-network structure, the problem that a traditional small-caliber artificial blood vessel is insufficient in mechanical performance is greatly solved, copper ions and magnesium ions are released to the surrounding environment by means of degradation of the magnesium-copper alloy particles in the using process, the microenvironment of the surrounding tissue is adjusted to be alkalescent, nitric oxide is catalytically released, and the mechanical performance of the artificial blood vessel is improved. The artificial blood vessel with the double-network structure is endowed with anticoagulation, endothelium promotion and anti-infection functions. The problems of restenosis and insufficient endothelialization of a small-caliber artificial blood vessel in the prior art are comprehensively solved, and the long-term patency rate of the artificial blood vessel is increased.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

Platelet lysate compositions and cell culture media compositions for maintaining and / or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and / or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and / or flasks pretreated with the cell culture media compositions.

The invention belongs to the technical field of biological medicines, and discloses a VEGFR-2 affinity peptide and application thereof, the VEGFR-2 affinity peptide can target a vascular endothelial growth factor receptor VEGFR-2, so that the VEGFR-2 affinity peptide can carry out in-vivo diagnosis on tumors with high expression of the VEGFR-2 receptor, and therefore, the VEGFR-2 affinity peptide can be used for in-vivo diagnosis of tumors with high expression of the VEGFR-2 receptor. The VEGFR-2 affinity peptide provided by the invention can be applied to preparation of a novel tumor diagnostic reagent or a novel tumor targeted drug carrier.

The invention discloses a preparation method of composite hydrogel for treating infectious wounds, which comprises the following steps: 1, dissolving double-bond modified recombinant collagen and a photoinitiator in deionized water containing vascular endothelial growth factor peptide under a dark condition to obtain a solution A; dissolving sulfhydrylated hyaluronic acid in deionized water containing the vascular endothelial growth factor peptide to obtain a solution B; and 2, mixing the solution A and the solution B, then adding an AgNO3 aqueous solution, carrying out vortex immediately, and then carrying out ultraviolet irradiation to obtain the composite hydrogel for treating infectious wounds. Thiolated hyaluronic acid and double-bond modified recombinant collagen are dissolved in an aqueous solution containing vascular endothelial growth factor peptide, an AgNO3 solution is added to enable Ag < + > and sulfydryl to be crosslinked, then photo-crosslinking is conducted to obtain the composite hydrogel, and the hydrogel has the advantages of being antibacterial, promoting cell proliferation and vascularization and the like and can be used for treating infectious wounds.

The present disclosure provides bispecific antibodies useful in gene therapy. Specifically, the invention provides a bispecific antibody, and the bispecific antibody comprises a heavy chain variable region (VHVEGF) and a light chain variable region (VLVEGF) which are targeted to a vascular endothelial growth factor (VEGF), and a heavy chain variable region (VHPD-L1) and a light chain variable region (VLPD-L1) which are targeted to a programmed death receptor-ligand 1 (PD-L1). The bispecific antibody provided by the disclosure can be used for gene therapy, such as AAV or mRNA mediated gene therapy, and can effectively block PD-1 / PD-L1 and VEGF / VEGFR pathways, and inhibit tumors in vitro and in vivo.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

Disclosed is an engineered exosome, comprising (a) a KGF polypeptide, fused to a first anchoring polypeptide, (b) a VEGF-Apolypeptide, fused to a second anchoring polypeptide, and (c) a Wnt family member polypeptide involved in Wnt / β-catenin signaling pathway, fused to a third anchoring polypeptide, wherein (a), (b) and (c) are anchored on a membrane of the exosome via the first, second and third anchoring polypeptide, respectively, and wherein the KGF polypeptide, the VEGF-Apolypeptide and the Wnt family member polypeptide are exposed on an outer surface of the membrane of the exosome. Also disclosed are compositions, nucleic acid constructs, and uses of the exosome in treating alopecia and / or canities.

The invention relates to the technical field of freeze-dried powder, in particular to a freeze-dried powder composition for hair regeneration and a preparation method thereof. The freeze-dried powder composition comprises 5-15 parts of a vascular endothelial growth factor, 3-10 parts of a platelet-derived growth factor, 1-5 parts of copper peptide, 0.5-3 parts of acetyl tetrapeptide-3, 2-8 parts of baicalein, 1-5 parts of asiaticoside, 20-40 parts of a freeze-drying protective agent and 1-3 parts of nano silicon dioxide. The preparation method comprises the following steps: mixing the pretreated vascular endothelial growth factor, platelet-derived growth factor, copper peptide, acetyl tetrapeptide-3, baicalein and asiaticoside with a freeze-drying protective additive and nano silicon dioxide, and freeze-drying. The freeze-dried powder composition can promote hair growth, improve scalp microenvironment and provide lasting support for healthy growth of hair. According to the preparation method, the stability and biological activity of the active ingredients are improved, and the overall performance and safety of the product are improved.

The invention discloses application of rapamycin combined with a vascular endothelial growth factor A in improvement of an ovarian tissue function. The improvement of the ovarian tissue function comprises any one or more than two of the following aspects: increasing the survival rate of follicles, prolonging the functional life of the ovarian tissue and improving the quality of oocytes. The effective concentration of the rapamycin is 500nM, and the effective concentration of the vascular endothelial growth factor A is 5ng / mL. The follicle loss dynamic after OTCT is tracked through a mouse model, it is found that rapid follicle loss is related to tip cell damage and primordial follicle activation, then a rapamycin and vascular endothelial growth factor A combination strategy is provided, follicle loss can be remarkably reduced, the reproductive function period can be prolonged, and the oocyte quality can be improved; according to the invention, a systematic solution is provided for OTCT technology optimization.

The invention relates to the field of medical instruments and public health, in particular to an artificial blood vessel with anticoagulation, endothelium promotion and bending resistance functions and a preparation method of the artificial blood vessel, and the artificial blood vessel is used for various blood vessel replacement and blood vessel bypass operations and has the characteristics of anticoagulation, endothelium promotion and bending resistance. The copper-containing material and the degradable magnesium-based metal material are respectively integrated on the inner surface of the artificial blood vessel and in the interlayer of the vessel wall, so that the functions of anticoagulation, endothelium promotion and bending resistance of the artificial blood vessel are realized. According to the artificial blood vessel with the anticoagulation, endothelium promotion and bending resistance functions, in the initial stage of use, due to the spiral structure of the magnesium alloy wire, the blood vessel cannot collapse after being bent, the blood flow is kept smooth, copper ions, magnesium ions and a small amount of hydrogen can be released to surrounding tissues in the use process, the microenvironment of the surrounding tissues is adjusted to be alkalescent, and the blood circulation is kept smooth. Through the synergistic effect of the copper ions and the magnesium alloy, the effects of promoting endothelialization, resisting blood coagulation and adjusting the acid-base property of the microenvironment are achieved at the same time, and therefore the functions of resisting blood coagulation, promoting endothelium and resisting bending are achieved.

The invention discloses an application of 4-octyl itaconic acid (4-OI) in preparation of a medicine for improving pulmonary arterial hypertension (PAH). In-vivo experiments show that 4-OI can significantly reduce pulmonary vascular wall thickening and collagen deposition of a PAH rat model, and effectively improve PAH and vascular remodeling. In-vitro experiments further reveal that 4-OI reduces endoplasmic reticulum stress (ERS) by inhibiting a PERK-eIF2alpha pathway, further blocks SM22 dependent smooth muscle cell phenotypic transformation and endothelial-mesenchymal transformation (EndMT) processes, finally inhibits vascular remodeling, and plays a role in treating PAH. The three key pathological processes of ERS, EndMT and vascular remodeling are closely linked, a new perspective is provided for deeply understanding the correlation among oxidative stress, endothelial dysfunction and vascular structure change in PAH, meanwhile, a theoretical foundation is laid for developing PAH and vascular remodeling accurate treatment drugs, and clinical transformation value is achieved.

Provided are compositions comprising aggregates of corneal endothelial cells (CECs) and methods administering said aggregates to patients for the treatment of corneal endothelial disease.

The invention discloses an anticoagulation endothelium slow release promoting coating and a preparation method thereof, and relates to the technical field of biomedical materials, the method comprises the following steps: soaking a substrate material in a guide layer solution prepared from dopamine, a silane coupling agent containing double bonds and an amino compound; immersing in an assembly layer solution prepared from a double-bond functionalized high-molecular compound and an endothelial active promoting substance, taking out, and carrying out photo-crosslinking curing; and immersing in a regulation and control layer solution prepared from a double-bond functionalized high-molecular compound and a zwitterionic monomer, and finally taking out for photo-crosslinking and curing to obtain the anti-coagulation endothelial sustained-release promoting coating. According to the method, efficient packaging and slow release of anticoagulation and endothelialization promoting active substances are achieved, and the prepared coating remarkably improves the performance of a blood contact instrument in the aspects of acute-stage thrombus formation resistance and long-acting endothelialization promotion after implantation.

Provided herein are methods of preparing an engineered heart valve for implantation into a subject in need (e.g., a pediatric patient). For example, a porcine pulmonary valve can be decellularized and then recellularized with cells from the subject. Hydrogels can be used to facilitate cell delivery and growth within the sub-endothelial layer (e.g., spongiosa) of a heart valve, which is essential for long-term success of the implantation.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention discloses a blood vessel adhesive bandage, a preparation method of the blood vessel adhesive bandage and application of the blood vessel adhesive bandage in promoting healing of a sutured port after a vascular surgical operation. An electrostatic spinning technology and a photo-crosslinking technology are combined to construct a high-porosity superfine methacrylic acid gelatin (GelMA) hydrogel fiber membrane patch; then tannic acid (TA) functional modified fiber surface is endowed with immunoregulation ability, finally, matrix-derived factor-1 alpha (SDF-1 alpha) is grafted to TA to endow the TA with stem cell collection ability, and finally, the SDF-1 alpha (at) TA (at) GelMA system patch is obtained. The patch can utilize SDF-1alpha to collect stem cells and gather the stem cells at a vascular suture to accelerate vascular endothelium regeneration, and TA is used for relieving local injury and performing immunoregulation, so that healing of a sutured wound is accelerated finally.