1079 results about "Endothelium" patented technology

The present invention relates to a helmet-type magnetic stimulation device comprising an inner sheath in contact with a skull part when the device is worn. The helmet-type magnetic stimulation device of the present invention is a helmet-type magnetic stimulation device for applying a magnetic field to nerve cells in the skull part or scalp cells so as to provide a non-invasive treatment, and includes a dome part providing a space in which the skull part is accommodated when the helmet-type stimulation device is worn; and an inner sheath inserted into the dome part to adjust the inner diameter of the dome part, wherein the inner sheath includes: a circumferential part disposed in an area which annularly surrounds the skull part in order to adjust the inner diameter of the dome part; and a connection part connected to one end and the other end of the circumferential part so as to connect the one end and the other end while crossing the upper side of an opening formed by the circumferential part.

Implants, implant systems, and methods for treatment of mitral valve regurgitation and other valve diseases generally include a coaptation assist body which remains within the blood flow path as the leaflets of the valve move, the valve bodies often being relatively thin, elongate (along the blood flow path), and / or conformable structures which extend laterally from commissure to commissure, allowing the native leaflets to engage and seal against the large, opposed surfaces on either side of the valve body during the heart cycle phase when the ventricle contracts to empty that chamber of blood, and allows blood to pass around the valve body so that blood flows from the atrium to the ventricle during the filling phase of the heart cycle. Separate deployment of independent anchors near each of the commissures may facilitate positioning and support of an exemplary triangular valve body, with a third anchor being deployed in the ventricle. An outer surface of the valve body may accommodate tissue ingrowth or endothelialization, while a fluid-absorbing matrix can swell after introduction into the heart. The valve body shape may be selected after an anchor has been deployed, and catheter-based deployment systems may have a desirable low profile.

The invention belongs to the technical field of medical instruments, and particularly relates to a dense net support. The dense net support comprises a front fixing section, a blocking section and a rear fixing section, and the blocking section is located between the front fixing section and the rear fixing section. The plugging section is connected with the front fixing section through a front connecting piece, and the plugging section is connected with the rear fixing section through a rear connecting piece; the plugging section is used for plugging an aneurysm position of a blood vessel, the plugging section sequentially comprises an inner layer part, a middle layer part and an outer layer part from inside to outside, the inner layer part adopts an inner layer metal stent, and the outer layer part adopts an outer layer metal stent. The dense net stent can adjust hemodynamics more accurately, and it is ensured that the blood flow of healthy blood vessels is not affected while the aneurysm is closed; the sealing effect of the aneurysm is ensured, and thrombosis is well promoted, so that blocking and healing of the aneurysm are promoted; and the endothelialization process of the stent is facilitated, so that the success rate of fusing the stent into the blood vessel is improved, the effect of completely sealing the aneurysm opening is achieved, and the recurrence risk is reduced.

The invention relates to anti-drug neutralizing antibody analysis. In particular, the present application relates to methods for detecting neutralizing antibodies against a vascular endothelial growth factor (VEGF) binding moiety and a programmed cell death ligand-1 (PD-L1) binding moiety in a biological sample from an individual administered a bispecific antibody drug comprising the VEGF binding moiety.

The invention discloses a method for quantitatively analyzing a left auricle occlusion effect based on a CT image. The method comprises the following steps: step 1, acquiring a heart CTA image; 2, performing three-dimensional reconstruction to obtain a heart three-dimensional image, and obtaining a left atrium three-dimensional image by using the left atrium segmentation model; 3, obtaining a left auricle occluder area by using the left auricle occluder segmentation model; 4, constructing a three-dimensional model of the left auricle occluder on the basis of the segmented left auricle occluder area so as to obtain a three-dimensional image of the left auricle occluder; 5, obtaining a three-dimensional plane of the left auricle plugging device, a front view of the plugging device and a left auricle opening plane; 6, left auricle occlusion postoperative evaluation parameters are extracted, wherein the parameters at least comprise the size, position and classification of residual shunt, the position of an occluder, the shoulder exposing position and height, the endothelialization degree of the occluder and the position of instrument-related thrombus; and 7, evaluating the left auricle occlusion effect according to the obtained evaluation parameters after the left auricle occlusion operation.

The invention discloses a vascular endothelial growth factor (VEGF) and antibacterial peptide (AMP) loaded chitosan methacrylic acid (CSMA) hydrogel microneedle patch as well as a preparation method and application thereof, the microneedle patch takes CSMA hydrogel as a main body material, the CSMA hydrogel is crosslinked and cured through ultraviolet light, and the microneedle patch has the characteristics of simplicity and convenience in preparation and stable material performance. The microneedle patch can continuously and stably release VEGF and AMP, promote wound healing and inhibit bacterial infection. Experiments show that the hydrogel microneedle is non-toxic to cells, has good biocompatibility and biodegradability, and also has excellent antibacterial performance. The invention provides an efficient and safe novel treatment tool for repairing infectious wounds.

The invention relates to a spontaneous cerebral hemorrhage postoperative intermuscular venous thrombosis risk prediction system based on a molecular mechanism and multi-modal data and a use method thereof, image space features are extracted through a multi-modal data level CNN to be complementary with time sequence features captured and detected by Transform, and support is provided for blood coagulation-endothelium-fibrinolysis three-channel activation degree calculation; a knowledge graph alignment module of a pathology and data level maps the cross-modal features to a unified semantic space, and corrects data conflicts with pathology time sequence constraints; and channel-space-time triple attention linkage at a space-time level is carried out, and a high-risk area is accurately marked. In a path driving layer, three-path activation degrees cooperatively adjust a dynamic time window, a single-path standard-exceeding triggering monitoring interval is shortened, and a double-path standard-exceeding amplitude is multiplied; and in a closed-loop feedback layer, system parameters are updated through confidence, pathological probability and conflict signal recursion, real-time calibration of a pathological mechanism and data is realized, and thrombus risk prevention and control efficiency is comprehensively improved.

The invention relates to medical recombinant collagen repair gel and a preparation method thereof, and belongs to the technical field of recombinant collagen. Through the synergistic effect of the collagen, the chitosan, the polylactic acid, the nanocellulose, the vascular endothelial growth factor, the anti-inflammatory drug and the cell adhesion peptide, the tissue repair effect is remarkably improved. Through a gas foaming method, staged freezing and freeze drying, multistage pore size distribution is generated, gel with a porous structure is formed, and cell permeation and tissue regeneration are facilitated; in addition, the permeability and the drug loading capacity of the gel are improved, the cell adhesion peptides, the growth factors and the anti-inflammatory drugs are better adsorbed to the gel, functional slow release is achieved, and the repairing effect is further improved.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention provides a construction method and application of a blood sinus networked liver organ. According to the method, pluripotent stem cells are co-differentiated into HAND1 < + > mesoderm and endoderm, a front intestine rear endoderm containing KDR < + > mesoderm progenitor cells is generated, a liver endoderm with CD34 < + > / CD31 < + > endothelial progenitor cells is generated, and the blood sinus networked liver organ is formed. The liver organ has a functional blood sinus endothelial network penetrating through the liver cluster, and has the characteristic functions of low-density lipoprotein uptake, human serum albumin removal, mediated secretion of blood coagulation factor 8 and the like. The method provides a new-generation highly bionic research tool for liver disease mechanism analysis, drug screening and regeneration repair.

The present invention relates to the field of stem cell technology. The present invention discloses a method for inducing stem cells to secrete specific factors, which includes stimulating stem cells with high-frequency waves, emitting sound waves of a specific frequency to stem cells, regulating stem cells in a hypoxic environment, culturing stem cells in a hypoxic environment, stimulating stem cells with oxidative stress, and subjecting stem cells to oxidative stimulation treatment. The present invention can significantly enhance the proliferation ability of stem cells, promote the secretion of multiple active factors, and improve cell function and adaptability. As a non-invasive physical method, high-frequency wave stimulation can activate internal cell signal pathways without introducing exogenous substances, significantly improving the proliferation ability and tissue repair potential of stem cells. Hypoxic environment regulation simulates the naturally existing microenvironmental conditions in the body, greatly promoting the secretion of active factors such as vascular endothelial growth factor, basic fibroblast growth factor, and brain-derived neurotrophic factor for tissue repair and immune regulation.

The invention relates to the technical field of biomedical engineering, and discloses a preparation method of a nano-enzyme SERS (Surface Enhanced Raman Scattering) sensor for detecting diabetic retinopathy, which comprises the following steps: (1) synthesizing a bimetallic gold-palladium nanorod; (2) assembling a gold trioctahedron array; and (3) modifying the surface of the gold-palladium nanorod synthesized in the step (1) with single-stranded DNA1 to prepare a nano-enzyme probe, hybridizing an aptamer of a vascular endothelial growth factor (VEGF) with single-stranded DNA2, modifying the surface of the gold trioctahedron array assembled in the step (2) with the hybridized aptamer, preparing a capture substrate, and constructing the nano-enzyme SERS sensor by the nano-enzyme probe and the capture substrate. In the detection process, the VEGF triggers a competitive recognition mechanism to promote the nano-enzyme probe to be captured by the capture substrate and catalyze tetramethyl benzidine (TMB) to generate oxTMB with a strong SERS signal. The sensor has the advantages of high sensitivity, strong specificity and good accuracy, and can realize rapid quantitative detection of DR biomarkers in serum.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention discloses a method for promoting endothelialization of a heart valve and an artificial heart valve.The method is characterized in that circular DNA is modified on the surface of a decellularized valve (DHV), DNA hydrogel of targeted thrombin is obtained on the surface of the DHV through rolling circle amplification, the DNA hydrogel plays a role of a heme catcher while playing a role of the heme catcher, and heme is recruited from blood; heme is further converted into biliverdin (BV) through heme oxygenase (HO1) loaded in DNA, so that continuous production of biliverdin is ensured, anti-inflammatory immune regulation and active oxygen removal are promoted, a regenerative immune microenvironment is created, and endothelialization of the valve is promoted.

The invention belongs to the technical field of electrochemical sensors, and particularly relates to an in-situ self-amplification nucleic acid-based flexible wound sensor and a preparation method thereof. The sensor comprises a flexible substrate, a gold layer and a nucleic acid sensing layer, the nucleic acid sensing layer comprises a tumor necrosis factor-alpha sensor, an interleukin 6 sensor, a transforming growth factor beta1 sensor, a vascular endothelial growth factor sensor, a temperature sensor, a pH sensor, a reference electrode and a counter electrode; the tumor necrosis factor-alpha sensor, the interleukin 6 sensor, the transforming growth factor beta1 sensor and the vascular endothelial growth factor sensor are all modified with rigid DNA tetrahedrons with hairpin DNA H2 and corresponding hairpin DNA H1. The sensor provided by the invention has antifouling and anti-degradation effects; finally, in-situ nucleic acid amplification on the flexible biosensing interface is realized by virtue of a hydrophilic / hydrophobic confinement effect and an auxiliary nucleic acid pre-fixation strategy, so that the detection sensitivity is improved.

The invention belongs to the field of biological medicines, and finds and verifies that taquinimod or pharmaceutically acceptable salts thereof can be used for preparing medicines for treating endometriosis for the first time, and is particularly suitable for progestogen-resistant, recurrent or deep infiltration type endometriosis. The action mechanism of the taquinimod is as follows: the taquinimod can down-regulate the expression of S100A9 protein and reduce the infiltration of S100A9 + macrophages by inhibiting an NF-kappa B signal channel so as to further inhibit the expression of a vascular endothelial growth factor receptor 1 (VEGFR1) and a matrix metalloproteinase 2 (MMP2), finally block pathological angiogenesis and inhibit the growth of ectopic lesions. Animal experiments show that the taquinimod can significantly reduce the number and weight of nidus of a mouse model with endometriosis. The invention provides a brand-new non-hormone treatment choice with a clear action mechanism for clinical application, provides a pharmaceutical composition containing a specific dosage and a drug combination scheme, and has an important clinical value.

The invention belongs to the technical field of cell mechanics biology experiment devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. The biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected, a micro-fluidic endothelial cell culture cavity and a peripheral system are constructed, real-time accurate control over aortic valve blood flow is achieved through the PID control system, and the system is suitable for large-scale popularization and application. Shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

Systems and methods relate to processing optical tomography coherence (OCT) images to predict characteristics of a treatment to be administered to effectively treat age-related macular degeneration. The processing can include pre-processing the image by flattening and / or cropping the image and processing the pre-processed image using a neural network. The neural network can include a deep convolutional neural network. An output of the neural network can indicate a predicted frequency and / or interval at which a treatment (e.g., anti-vascular endothelial growth factor therapy) is to be administered so as to prevent leakage of vasculature in the eye.

The present disclosure relates to fusion molecules and chimeric molecules which comprise two components: an Ang-2 antagonist peptide linked to a VEGF-binding moiety. Further disclosed are methods of using said chimeric molecules to treat a patient cancer, proliferative retinopathy, neovascular glaucoma, macular edema, wet age-related macular degeneration (wAMD), macular edema following retinal vein occlusion (RVO), diabetic macular edema (DME), or diabetic retinopathy (DR).

The invention relates to the technical field of biomedical materials, in particular to a double-network structure artificial blood vessel with anticoagulation, endothelium promotion and anti-infection functions and a preparation method of the double-network structure artificial blood vessel. The artificial blood vessel comprises a polymer fiber network artificial blood vessel substrate, a microscopic network hydrogel layer penetrating through the polymer fiber network artificial blood vessel substrate, and magnesium-copper alloy particles doped in the hydrogel layer. According to the artificial blood vessel with the double-network structure, the problem that a traditional small-caliber artificial blood vessel is insufficient in mechanical performance is greatly solved, copper ions and magnesium ions are released to the surrounding environment by means of degradation of the magnesium-copper alloy particles in the using process, the microenvironment of the surrounding tissue is adjusted to be alkalescent, nitric oxide is catalytically released, and the mechanical performance of the artificial blood vessel is improved. The artificial blood vessel with the double-network structure is endowed with anticoagulation, endothelium promotion and anti-infection functions. The problems of restenosis and insufficient endothelialization of a small-caliber artificial blood vessel in the prior art are comprehensively solved, and the long-term patency rate of the artificial blood vessel is increased.

The invention discloses a stem cell preparation for treating pulmonary fibrosis. The stem cell preparation is mesenchymal stem cell surface modified nanoparticles. The nanoparticles are ROS (reactive oxygen species) response type lipid polymer hybrid nanoparticles loaded with an anti-fibrosis drug. According to the invention, a hydrophobic drug and a hydrophilic anti-fibrosis drug can be simultaneously loaded through the reductive hybrid nanoparticles, and can be delivered to the lung by utilizing the inflammation targeting capability of stem cells. The polymer in the nanoparticles reacts with active oxygen in the microenvironment to promote drug release, and then the effect of protecting stem cells is achieved. The released medicine can induce dedifferentiation of myofibroblasts and inhibit apoptosis of vascular endothelial cells and endothelial mesenchymal transformation, and the mesenchymal stem cells release growth factors at the same time to promote angiogenesis, reconstruct capillary structures deleted in fibrosis areas, promote lung tissue regeneration and recover lung functions to achieve the effect of treating pulmonary fibrosis.

The invention discloses a double-layer small-caliber artificial blood vessel with an in-situ modified anticoagulant coating and a preparation method. The double-layer small-caliber artificial blood vessel with the in-situ modified anticoagulant coating is prepared by combining a coaxial dry-jet wet spinning technology and a polydopamine-heparin modification technology. According to the obtained double-layer small-caliber blood vessel, the outer layer provides enough mechanical strength and structural stability, the inner layer has good hydrophilicity and excellent anticoagulation function through polydopamine in-situ grafting heparin, activation of platelets and adhesion of blood cells are reduced, formation of short-term acute thrombus is prevented, and the endothelialization process of the small-caliber blood vessel is promoted; according to the method provided by the invention, the manufacturing and surface modification process of the small-caliber artificial blood vessel is simplified, additional procedures such as secondary processing are avoided, the modification position and the grafting amount are controllable, the experimental stability is high, and a convenient and efficient preparation technical idea is provided for developing the small-caliber artificial blood vessel.

The present disclosure provides a construct comprising a coding sequence operably linked to a promoter, wherein the coding sequence encodes a vascular endothelial growth factor (VEGF) binding agent or a portion thereof. In some embodiments, a construct is an AAV construct. In some embodiments, an AAV construct is a part of an AAV particle. Compositions comprising constructs and AAV particles described herein can be useful in treating hearing loss, for example, hearing loss associated with vestibular schwannoma.

The invention provides an anti-inflammatory and anti-vascular bidirectional trapping combined self-luminous power compound based on neutrophil nano-vesicles as well as a preparation method and application thereof, and belongs to the technical field of biological medicine manufacturing. The compound takes a neutrophil nano-vesicle as a carrier, and is also loaded with bis (2, 4, 5-trichlorosalicylic acid n-amyl ester) oxalate (CPPO) and a photosensitizer Ce6, and meanwhile, the surface of the compound is modified with ranibizumab through ketal mercaptan responded by ROS (reactive oxygen species). The compound has the characteristics of trapping anti-inflammatory factors and spontaneously targeting corneal neovascularization diseased regions, Ranibizumab is responsively released on the basis of ROS to be combined with vascular endothelial growth factors, the anti-angiogenesis function is achieved, meanwhile, chemical energy generated by CPPO under the high-oxygen condition stimulates Ce6 to generate singlet oxygen, and therefore the vascular endothelial cell tubulogenesis capacity is inhibited. The invention provides a new means for treatment of eye disease related diseases.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

Platelet lysate compositions and cell culture media compositions for maintaining and / or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and / or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and / or flasks pretreated with the cell culture media compositions.

The invention discloses a fucoidin-bioactive peptide coated intravascular stent and a preparation method thereof, and belongs to the technical field of medical treatment. The surface of the fucoidin-bioactive peptide coating intravascular stent is coated with a composite coating, and the composite coating is composed of fucoidin and bioactive peptide. The invention discloses a fucoidin-bioactive peptide coated intravascular stent and a preparation method thereof, and the fucoidin-bioactive peptide coated intravascular stent not only solves the problems of poor blood compatibility and slow endothelialization, but also has multiple functions of anticoagulation, anti-proliferation and repair promotion. The intravascular stent coating technology is comprehensively upgraded, and a safer, more effective and more lasting solution is provided for treatment of cardiovascular diseases.

The invention belongs to the technical field of biological medicines, and discloses a VEGFR-2 affinity peptide and application thereof, the VEGFR-2 affinity peptide can target a vascular endothelial growth factor receptor VEGFR-2, so that the VEGFR-2 affinity peptide can carry out in-vivo diagnosis on tumors with high expression of the VEGFR-2 receptor, and therefore, the VEGFR-2 affinity peptide can be used for in-vivo diagnosis of tumors with high expression of the VEGFR-2 receptor. The VEGFR-2 affinity peptide provided by the invention can be applied to preparation of a novel tumor diagnostic reagent or a novel tumor targeted drug carrier.

The invention discloses a preparation method of composite hydrogel for treating infectious wounds, which comprises the following steps: 1, dissolving double-bond modified recombinant collagen and a photoinitiator in deionized water containing vascular endothelial growth factor peptide under a dark condition to obtain a solution A; dissolving sulfhydrylated hyaluronic acid in deionized water containing the vascular endothelial growth factor peptide to obtain a solution B; and 2, mixing the solution A and the solution B, then adding an AgNO3 aqueous solution, carrying out vortex immediately, and then carrying out ultraviolet irradiation to obtain the composite hydrogel for treating infectious wounds. Thiolated hyaluronic acid and double-bond modified recombinant collagen are dissolved in an aqueous solution containing vascular endothelial growth factor peptide, an AgNO3 solution is added to enable Ag < + > and sulfydryl to be crosslinked, then photo-crosslinking is conducted to obtain the composite hydrogel, and the hydrogel has the advantages of being antibacterial, promoting cell proliferation and vascularization and the like and can be used for treating infectious wounds.

The present disclosure provides bispecific antibodies useful in gene therapy. Specifically, the invention provides a bispecific antibody, and the bispecific antibody comprises a heavy chain variable region (VHVEGF) and a light chain variable region (VLVEGF) which are targeted to a vascular endothelial growth factor (VEGF), and a heavy chain variable region (VHPD-L1) and a light chain variable region (VLPD-L1) which are targeted to a programmed death receptor-ligand 1 (PD-L1). The bispecific antibody provided by the disclosure can be used for gene therapy, such as AAV or mRNA mediated gene therapy, and can effectively block PD-1 / PD-L1 and VEGF / VEGFR pathways, and inhibit tumors in vitro and in vivo.