19 results about "Human epidermal growth factor" patented technology

The invention belongs to the technical field of cell culture, and particularly relates to a culture method and application of human astrocytes. According to the culture solution provided by the invention, DMEM (Dulbecco Modified Eagle Medium) and MCDB 131 are mixed according to a specific ratio to form a basic culture medium, and functional additives of HEPES, Glutamax, N2, G-5, B-27, hEGF (Human Epidermal Growth Factor) and the like with accurate concentration and fetal calf serum with proper concentration are matched. The culture solution can effectively improve the vitality and proliferation efficiency of SVGP12 cells, guarantees the growth stability and morphological consistency of the cells, can provide a high-quality and reliable cell source for the application of human astrocytes in the fields of neurobiology research, neurological disease mechanism exploration, drug screening and the like, and has important practical value.

This invention discloses an antibody against human epidermal growth factor 3 (HER3), a method for producing the same, and its use. The HER3 antibody specifically binds to HER3, is endocytotic by cells, and can be used to suppress diseases associated with HER3 expression (including overexpression) or affected by HER3 function, such as tumors.

The invention provides a composition containing neural stem cells, which comprises the following components in parts by mass: 15-20 parts of neural stem cells which are subjected to hypoxia pretreatment and chemical protective agent treatment; 30 to 40 parts of a collagen-sodium alginate composite carrier; 5-8 parts of compound active factors, wherein the compound active factors comprise endostatin, recombinant human epidermal growth factors and superoxide dismutase; and 100 parts of cell protection liquid, wherein the cell protection liquid comprises thioredoxin and nicotinamide. The composition is used for preparing a medicine for treating retina degenerative diseases, and the medicine is an intravitreal injection preparation. The composition synchronously overcomes the three technical bottlenecks of low cell transplantation survival rate, out-of-control differentiation direction and neural network reconstruction disorder through neural stem cell hypoxia pretreatment, active factor compound network, collagen-sodium alginate carrier mechanical adaptation and multi-channel synergistic intervention of a redox protection system.

The application discloses a preparation method and a treatment device of recombinant human epidermal growth factor silk membrane, relates to the technical field of biological products, and is characterized in that a transgenic epidermal growth factor silkworm strain N S EC S The transgenic epidermal growth factor silk biological membrane dressing is prepared through the technical processes of hot pressing and sterilization after the flat plate silk is spun on the two-dimensional plane of a mold. The transgenic epidermal growth factor silk biological membrane prepared by using the technology can greatly maintain the content and activity of the growth factor, greatly saves the time required for preparing the silk structure biological membrane dressing, maintains the natural structure and mechanical properties of the silk, and the preparation process is simpler. Since degumming and silk fibroin extraction are not needed, the treatment cost of the degumming liquid and the silk fibroin extraction liquid is reduced, the pollution to the environment is reduced, and the problem of maintaining the natural structure of the silk and the activity of the epidermal growth factor in the preparation process of the traditional silk membrane is solved. The human epidermal growth factor silk membrane prepared by the method can be applied to the medical fields of wound repair and tissue repair, and has good application potential and value.

The invention discloses a high-stability hEGF sustained-release gel external preparation and a preparation method, and belongs to the technical field of sustained-release gel preparations, and the preparation method comprises the following steps: preparing a poloxamer 407 solution, and preparing a sodium carboxymethyl cellulose solution; adding the sodium carboxymethyl cellulose solution into the poloxamer 407 solution to obtain a mixed solution A, preparing a borneol solution, and adding the borneol solution into the mixed solution A to obtain a mixed solution B; adding the human epidermal growth factor into the mixed solution B to obtain a mixed solution C; filtering and sterilizing to obtain the hEGF sustained-release gel preparation. By adopting a low-temperature gel drug-loading combined process and cooperating with a stable pH system, the biological activity of the human epidermal growth factor can be effectively maintained, it is ensured that the preparation can normally exert the core biological functions of regulating and controlling proliferation, differentiation, migration and the like of related target cells, and the basic requirements for drug efficiency in practical application are met.

The present application provides a kind of for human epidermal growth factor 2 (HER2) and integrin dual targeting binding protein and its production method and application.Integrin dual targeting binding protein includes HER2 specific nanobody or single chain antibody and integrin polypeptide ligand (RGD).Dual targeting antigen binding protein can target recognition HER2 and integrin protein specifically expressed on the surface of tumor cell, interfere with the expression and signal transduction of HER2 and integrin protein, while inhibiting the expression of EGFR, HER2, HER3, HER4, so as to maximum degree inhibit the growth signal pathway of tumor cell.The dual targeting binding protein can also be used as a drug carrier to deliver drugs directly to the tumor site.The dual targeting binding protein described in the present application can be prepared by E. coli expression system.

The application relates to the technical field of stem cells, in particular to a mesenchymal stem cell culture system without heterogeneity and application thereof. The mesenchymal stem cell culture system without heterogeneity comprises a basic culture medium and an additive component, wherein the basic culture medium is DMEM / F12, and the additive component comprises: recombinant human transferrin, recombinant human epidermal growth factor, recombinant human basic fibroblast growth factor, recombinant human insulin, recombinant human interferon gamma, hydrocortisone, vitamin A, vitamin D2, vitamin B, human blood albumin, CTS KnockOut SR XenoFree, glutamine and dextran. The mesenchymal stem cell culture system without heterogeneity provided by the application improves the proliferation efficiency of primary culture and in-vitro expansion culture of mesenchymal stem cells, is high in safety, good in stability and suitable for large-scale popularization and application.

The invention discloses a human epidermal growth factor temperature-sensitive sustained-release gel as well as a preparation method and application thereof, and belongs to the technical field of biological pharmacy. The preparation method of the human epidermal growth factor temperature-sensitive sustained-release gel comprises the following steps: S1, adding poloxamer and a thickening agent into purified water, and uniformly mixing to form a gel matrix; s2, respectively preparing a human epidermal growth factor solution and a chitosan-sodium tripolyphosphate colloid, and adding the human epidermal growth factor solution into the chitosan-sodium tripolyphosphate colloid for adsorption to obtain the chitosan-sodium tripolyphosphate colloid loaded with the human epidermal growth factor; s3, adding the chitosan-sodium tripolyphosphate colloid loaded with the human epidermal growth factors, a humectant and a stabilizer into the gel matrix, and adjusting the pH value. The gel can realize stable loading and controllable slow release of the human epidermal growth factor, so that the administration frequency and repeated administration operation are reduced, and the gel has important application value in treatment of refractory wounds.

The present invention relates to a transformed Pediococcus pentosaceus strain into which a gene encoding a human epidermal growth factor (EGF) protein is introduced to express and secrete an epidermal growth factor, and a skin-improving cosmetic composition comprising same. According to the present invention, whitening, wrinkle improvement, antioxidant, and skin regeneration effects superior to those of a cosmetic composition comprising commercially available isolated / purified EGF are exhibited. In addition, the present invention facilitates large-scale production of EGF and has economical production costs, thereby providing high competitiveness as a raw material for cosmetic compositions.

The invention discloses a co-culture solution, a construction method of an in-vitro organ-like model, the in-vitro organ-like model and application of the in-vitro organ-like model, and belongs to the technical field of biological medicine. The DMEM culture medium contains 4-hydroxyethylpiperazine ethanesulfonic acid, L-alanyl-L-glutamine, an N2-additive, a G-5 additive, a B-27 additive, a human epidermal growth factor, penicillin-streptomycin and fetal calf serum, and the MCEB131 culture medium is used for culturing the human epidermal growth factor, the penicillin-streptomycin and the fetal calf serum. The co-culture solution provided by the invention can realize co-culture of human astrocytes SVGP12, human microvascular endothelial cells HMEC-1 and human perineuronal cells HBVP, avoids the interference of cross components of different nutrient solutions on the cells, and improves the stability of an in-vitro organ-like model.

The invention relates to the technical field of sludge conditioners, in particular to a pharmaceutical composition for preventing and treating oropharyngeal mucosal injury caused by radiotherapy and chemotherapy, which is prepared from forsythin, radix ophiopogonis total saponins, L-glutamine, famciclovir, yeast beta-glucan, recombinant human epidermal growth factors, glutathione and glycine. The oral pharyngeal mucosal injury caused by chemoradiotherapy can be prevented and treated. According to the traditional Chinese medicine composition, multiple components have a synergistic effect, and the traditional Chinese medicine composition has prevention and treatment effects and is free of incompatibility and good in tolerance; the mucous membrane adhesive force is enhanced, the administration frequency is reduced, the dosage forms are diversified, different injury degrees and medication scenes are adapted, and the clinical prevention and control requirements are comprehensively met.

The invention relates to the field of diabetic wound treatment, and particularly discloses a medicine for treating diabetic wound healing and a preparation method thereof.The medicine comprises active ingredients and a pharmaceutically acceptable carrier, the active ingredients are composed of epigallocatechin gallate, recombinant human epidermal growth factors and sitagliptin, and the carrier is composed of chitosan, chitosan and chitosan. The mass ratio of the three components is (20-50): (1-5): (5-15); according to the invention, through innovative compatibility of the active components epigallocatechin gallate (EGCG), recombinant human epidermal growth factor (rhEGF) and sitagliptin, the three components generate a synergistic interaction effect exceeding simple addition; eGCG strongly clears active oxygen and inhibits formation of advanced glycosylation end products, sitagliptin regulates immune response and improves local microenvironment, and clears obstacles for efficient playing of cell proliferation and migration promoting functions of rhEGF, so that drugs can synchronously intervene from three dimensions of oxidation resistance, inflammation resistance and growth promotion.

Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.

The present invention relates to new proteins that are specific for human epidermal growth factor kinase receptor 3 (ERB-B3 receptor tyrosine kinase 3, Her3). The Her3 specific proteins of the invention bind with high affinity to human Her3. The invention further refers to Her3 specific proteins that further comprise a diagnostically or therapeutically active component. Further aspects of the invention cover the use of these Her3 specific binding proteins in medicine, for example, in diagnosis and therapy of Her3 related cancer.

The application discloses a high-stability hEGF sustained-release gel external preparation and a preparation method, and belongs to the technical field of sustained-release gel preparations, and comprises the following steps: preparing a poloxamer 407 solution, preparing a sodium carboxymethyl cellulose solution; adding the sodium carboxymethyl cellulose solution into the poloxamer 407 solution to obtain a mixed solution A, preparing a borneol solution, adding the borneol solution into the mixed solution A to obtain a mixed solution B; adding human epidermal growth factor into the mixed solution B to obtain a mixed solution C; and filtering and sterilizing to obtain the hEGF sustained-release gel preparation. The low-temperature gel drug loading combination process is adopted in combination with a stable pH system, so that the biological activity of the human epidermal growth factor can be effectively maintained, the preparation can normally play its core biological functions of regulating the proliferation, differentiation and migration of related target cells, and the basic demand for drug efficiency in actual application is met.

The invention discloses a primary tissue or organ-like cryopreservation solution which is prepared from dimethyl sulfoxide, dextran-20, primary cell antibiotics, Penicillin-streptomysin, bovine serum albumin, insulin, transferrin, growth hormone, fibroblast growth factors, human platelet growth factors, bone morphogenetic protein 4, human epidermal growth factors, insulin-like growth factors and the balance of distilled water. The culture medium is prepared from a hepatocyte growth factor, L-glutamic acid, alanine, glycine, L-asparaginic acid, L-proline, serine, sodium pyruvate, glutamine, nicotinamide, N-acetylcysteine, beta-mercaptoethanol, sodium selenite, beta glycerophosphate, cycloastragenol, Y-27632, a DMEM (Dulbecco Modified Eagle Medium) basal culture medium and the like. After the cryopreserved tissue is recovered, the isolated culture of the primary cells can be normally carried out, the viability of the primary cells reaches 93%, and the survival rate of the recovered organoid reaches 92%.

The invention provides a nursing pharmaceutical composition for preventing pressure sores and a preparation method thereof, and the composition is composed of an active component, a liquid absorption component and pharmaceutically acceptable auxiliary materials, active components of the composition comprise an astragalus extract, an angelica sinensis extract, a lithospermum extract, a recombinant human epidermal growth factor, pentoxifylline, polyhexamethylene biguanide hydrochloride, beta-glucan, asiaticoside and magnolol, and the effects of improving microcirculation, resisting inflammation and bacteria and promoting tissue repair are comprehensively achieved through a multi-target synergistic effect; luminan microspheres or calcium alginate fibers are adopted as liquid absorption components, wound exudate is effectively managed, a wet environment is maintained, a split-phase preparation and low-temperature integration process is adopted in the preparation method, the compatibility problem of complex components is solved, and the stability and effectiveness of the product are ensured; the device integrates active treatment and protection, is suitable for preventing and treating pressure sores in all stages, and has the advantages of being remarkable in effect and safe to use.

The invention relates to serum protein essence for promoting hair follicle repair and scalp care and a preparation method thereof, and belongs to the technical field of daily chemical products. The serum protein essence comprises the following components in percentage by weight: 0.005-0.02% of human epidermal growth factor (EGF), 6-10% of hydrolyzed silk protein, 2-5% of nicotinamide, 1-3% of trehalose and the balance of a buffer solution. According to the product, hair follicle stem cells are directly activated through small molecule EGF, silk protein is hydrolyzed to form an epidermis protection layer, nicotinamide enhances the skin barrier function, and trehalose prolongs the activity of components; the whole formula is stably dispersed through a micro-emulsification system, and the synergistic effect of the components is guaranteed. Experiments prove that the hair follicle density can be remarkably increased, the percutaneous water loss rate can be improved, and the stability of active ingredients can be improved.