12 results about "Elisa test" patented technology

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

The application discloses application of a reagent for detecting AHSP protein in preparation of a product for diagnosing prostate cancer, and relates to the technical field of biological medicine; through proteomic analysis, ELISA test on urine protein of prostate cancer patients, clear cell renal cell carcinoma patients and normal healthy men, and immunohistochemical analysis on file wax blocks of the prostate cancer patients, normal prostate tissues around the cancer, prostate tissues of prostatic hyperplasia and clear cell renal cell carcinoma tissues, it is found that the AHSP protein has a certain relationship with the occurrence and development of prostate cancer, and can be used for assisting diagnosis of the prostate cancer; and in urine detection, AHSP has higher sensitivity than PSA, and when PSA is negative or serum PSA is low, AHSP can form complementation with ASHP, thereby helping to improve the diagnosis rate and non-invasive screening ability; through research on the relationship between the AHSP protein and the prostate cancer, the diagnosis rate of patients can be improved, non-invasive reexamination after treatment is facilitated, drug use is guided, and the survival rate is improved.

The application discloses an anti-IL-10 monoclonal antibody and a preparation method and application thereof, discloses the anti-IL-10 monoclonal antibody, the preparation method of the antibody, the application of the antibody in the preparation of drugs for various diseases, and further discloses the application of the antibody provided by the application in pairing use for detecting antigens.The anti-IL-10 monoclonal antibody of the application can be specifically combined with IL-10 antigens or sIL-10 antigens, lays a foundation for diagnosing or treating or preventing viral infections, tumors or inflammatory diseases by a genetic engineering method; the antibody can also be used in pairing, and the sIL-10 concentration in serum can be accurately measured and detected; in addition, the anti-IL-10 monoclonal antibody disclosed by the application has a specific antigen binding domain, can be targeted to combine with IL-10 proteins, and can also be used in immunohistochemical tests, ELISA tests and the like.

The invention discloses an application of intestinal flora metabolite salicylic acid as a predictive marker of Escherichia coli calf diarrhea. The obviously up-regulated metabolite salicylic acid is screened out from the diarrhea calf feces through a non-targeted metabonomics technology, and in a group of new independent samples, it is verified through an ELISA test that the salicylic acid concentration of the diarrhea calf feces is obviously higher than that of a healthy group. ROC curve analysis shows that the area under curve (AUC) of SA for predicting escherichia coli calf diarrhea is 0.8148, and the method has good prediction capability. The invention provides a non-invasive, cheap, rapid and high-specificity prediction method, can be used for early warning and auxiliary diagnosis of Escherichia coli calf diarrhea, and has important significance for guiding clinical precise medication and reducing the death rate of calves.

The application discloses a method for relieving the adverse effects of chronic restraint stress on animals by adding sodium isovalerate into feed, which comprises taking C57BL / 6J mice as research objects, studying the effects of sodium isovalerate on growth performance, depression behavior and intestinal barrier and inflammation indexes of the mice with chronic restraint stress, recording daily weight gain and feed intake, and performing tail suspension test and Elisa test and other test methods, and proving that sodium isovalerate can relieve the decrease of mouse weight growth rate, the increase of feed to weight ratio and the decrease of organ index caused by CRS; through fluorescence quantitative PCR and Western Blot and other methods, it is proved that sodium isovalerate can relieve the intestinal injury of the CRS mice, reduce the expression of colon pro-inflammatory factors and improve the expression of tight junction proteins, and can also reduce the phosphorylation level of NF-kappa B in the colon of the CRS mice. The application explores the relieving effect of sodium isovalerate on chronic restraint stress of animals, so as to provide some theoretical basis for relieving stress in actual production and the application of sodium isovalerate in feed additives.

The application discloses a pig IZUMO2 eukaryotic protein and a preparation method and application of a monoclonal antibody of the pig IZUMO2 eukaryotic protein. The full-length sequence of pig IZUMO2 nucleotide CDS is verified, a research method for removing the transmembrane structure of the pig IZUMO2 nucleotide CDS and only expressing the extramembrane sequence of the pig IZUMO2 nucleotide CDS in eukaryotes is designed, and the IZUMO2 protein is expressed and secreted by CHO-K1 cells. The protein expressed by the method is close to natural protein, has biological activity and is simple to operate. The qPCR and ELISA tests verify that the IZUMO2 protein can reduce the expression of inflammatory factors in RAW264.7 cells induced by LPS, and has a certain anti-inflammatory capacity. It is found through experiments that the IZUMO2 protein can promote the capacitation of boar sperm, and it is proved that the protein can be applied to the preparation of a reagent for promoting the capacitation of boar sperm.

The utility model provides an incubator for an ELISA (enzyme-linked immunosorbent assay) test in a veterinary laboratory. The incubator comprises a box body, the box door is arranged on one side of the front surface of the box body, and a drawer type elisa plate frame is arranged on the surface of the box door. According to the incubator for the ELISA test in the veterinary laboratory, the electric heater is placed in the water tank to heat water, so that the temperature in the incubator is increased; the stainless steel inner container is provided with a micro fan and a grid circulating air port, and the temperature in all directions in the box is rapidly balanced through air flow; by using the drawer type elisa plate rack, the elisa plate can be taken and placed without opening and closing the box door, the incubator is in a sealed state, heat loss is reduced, the temperature in the incubator is relatively stable, the incubator is still in a sealed state, heat loss is reduced, temperature fluctuation in the incubator caused by door opening is greatly reduced, the size of the incubator is small, space can be saved, and the incubator is convenient to use. And the operation of experimenters is facilitated.

The present invention discloses a formula of influenza virus maintenance solution and its preparation method and application, and specifically relates to the field of biomedicine technology. The virus maintenance solution includes DMEM / F-12 basal culture medium and active ingredients, and the active ingredients include amino acids, vitamins, salts, virus proliferation promoters and additives, wherein the virus proliferation promoter includes yeast extract. The present invention develops an influenza virus maintenance solution formula based on DMEM / F-12 basal culture medium by establishing an influenza virus subunit ELISA detection kit and taking the ELISA test result at the culture end point as the response value. The virus yield of influenza virus on MDCK-v cell matrix can be significantly improved, and production costs can be reduced.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

The invention discloses a method for determining the binding activity of epidermal growth factors in sodium hyaluronate composite gel. The method comprises the following steps: S1, weighing HE and carrying out enzymolysis to obtain a pre-diluent of a test sample; s2, weighing a human epidermal growth factor activity determination standard substance and redissolving to obtain a linear standard substance solution; s3, diluting the pre-diluent to enable the pre-diluent to be within a detection linear range to obtain a test solution; s4, incubating the standard solution and the test solution; s5, adding a color developing solution after incubation, incubating in a dark place, and adding a stop solution after color development; s6, putting into a microplate reader, measuring absorbance, and recording a result; and S7, drawing a standard curve and analyzing the sample concentration according to the result, and calculating the percentage of the sample concentration in the theoretical value. According to the invention, sodium hyaluronate enzyme is added, so that sodium hyaluronate in the gel can be subjected to enzymolysis, the constraint of the sodium hyaluronate hydrogel with a net structure on EGF in a test sample is relieved, the influence of a double-antibody one-step sandwich method ELISA test on the detection of the EGF content in HE is reduced, and the accuracy of a detection result is improved.

The application belongs to the technical field of biological detection, and discloses a bovine nodular dermatopathy virus specific competitive ELISA detection kit and application thereof. The application discloses a hybridoma cell strain LSDV-ORF132-11H1, and the application realizes high-efficiency soluble expression of LSDV-ORF132 based on a prokaryotic expression system. After mice are immunized by taking LSDV-ORF132 as an immunogen, a LSDV positive hybridoma cell strain LSDV-ORF132-11H1 capable of stably secreting antibodies is successfully prepared and screened after cell fusion. The ELISA titer of the antibody obtained after the monoclonal antibody secreted by the cell strain is purified is 1:64000, the heavy chain of the antibody is an IgGa2 subtype, and the light chain is a Kappa chain. Based on the antibody, high-efficiency detection of bovine nodular dermatopathy virus ORF132 protein can be realized.

The utility model discloses an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit, which belongs to the technical field of biological detection equipment and comprises a microwell plate, a box body and a top cover, the box body and the top cover are matched with each other, an inner box is movably connected in the box body, a buffer component is arranged between the box body and the inner box, and a partition plate is fixedly connected in the middle of the inner box. A supporting net for placing the micro-pore plate is fixedly connected into the inner box above the partition plate, and a vibration assembly is fixedly mounted at the bottom of the inner box. According to the ELISA detection kit, the supporting net is used for supporting the microwell plate, the top cover is matched with the pressing block and the elastic assembly to fix the microwell plate, then the vibration assembly is matched to vibrate the inner box and the microwell plate, waste liquid passes through the supporting net, and the inner box is matched with the partition plate to temporarily store the waste liquid. The problem that a worker needs to hold the kit to move back and forth among the placing unit, the vibration unit and the waste liquid cylinder, and time and labor are wasted is solved, and the detection efficiency is improved.