7 results about "Elisa test" patented technology

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

The application discloses application of a reagent for detecting AHSP protein in preparation of a product for diagnosing prostate cancer, and relates to the technical field of biological medicine; through proteomic analysis, ELISA test on urine protein of prostate cancer patients, clear cell renal cell carcinoma patients and normal healthy men, and immunohistochemical analysis on file wax blocks of the prostate cancer patients, normal prostate tissues around the cancer, prostate tissues of prostatic hyperplasia and clear cell renal cell carcinoma tissues, it is found that the AHSP protein has a certain relationship with the occurrence and development of prostate cancer, and can be used for assisting diagnosis of the prostate cancer; and in urine detection, AHSP has higher sensitivity than PSA, and when PSA is negative or serum PSA is low, AHSP can form complementation with ASHP, thereby helping to improve the diagnosis rate and non-invasive screening ability; through research on the relationship between the AHSP protein and the prostate cancer, the diagnosis rate of patients can be improved, non-invasive reexamination after treatment is facilitated, drug use is guided, and the survival rate is improved.

The invention discloses an application of intestinal flora metabolite salicylic acid as a predictive marker of Escherichia coli calf diarrhea. The obviously up-regulated metabolite salicylic acid is screened out from the diarrhea calf feces through a non-targeted metabonomics technology, and in a group of new independent samples, it is verified through an ELISA test that the salicylic acid concentration of the diarrhea calf feces is obviously higher than that of a healthy group. ROC curve analysis shows that the area under curve (AUC) of SA for predicting escherichia coli calf diarrhea is 0.8148, and the method has good prediction capability. The invention provides a non-invasive, cheap, rapid and high-specificity prediction method, can be used for early warning and auxiliary diagnosis of Escherichia coli calf diarrhea, and has important significance for guiding clinical precise medication and reducing the death rate of calves.

The application discloses a pig IZUMO2 eukaryotic protein and a preparation method and application of a monoclonal antibody of the pig IZUMO2 eukaryotic protein. The full-length sequence of pig IZUMO2 nucleotide CDS is verified, a research method for removing the transmembrane structure of the pig IZUMO2 nucleotide CDS and only expressing the extramembrane sequence of the pig IZUMO2 nucleotide CDS in eukaryotes is designed, and the IZUMO2 protein is expressed and secreted by CHO-K1 cells. The protein expressed by the method is close to natural protein, has biological activity and is simple to operate. The qPCR and ELISA tests verify that the IZUMO2 protein can reduce the expression of inflammatory factors in RAW264.7 cells induced by LPS, and has a certain anti-inflammatory capacity. It is found through experiments that the IZUMO2 protein can promote the capacitation of boar sperm, and it is proved that the protein can be applied to the preparation of a reagent for promoting the capacitation of boar sperm.

The invention discloses a multi-target fluorescence immunoassay method based on TMSD-DNAzyme, which is used for simultaneously detecting staphylococcus aureus enterotoxin B (SEB) and GII type norovirus (NoV), and comprises the following steps: S1, coating a 96-hole black microplate with an SEB capture antibody, and sealing; s2, a diluted immune probe and a sample to be detected are added, a sandwich structure is formed through an immune reaction, and the immune probe is formed by coupling an SEB detection antibody and a Trigger chain on the surface of a gold nanoparticle; according to the invention, by designing two sets of independent signal amplification channels which can run in parallel, the detection of SEB protein and NoV nucleic acid is completed in one reaction hole at the same time, compared with a traditional mode needing two independent ELISA detection, the method has the advantages that the detection process is simplified by nearly one time, the real'one-time detection and double results' is realized, and the detection efficiency is greatly improved. The detection flux is greatly improved, and about 50% of sample, reagent and time cost is saved.

The application belongs to the technical field of biological detection, and discloses a bovine nodular dermatopathy virus specific competitive ELISA detection kit and application thereof. The application discloses a hybridoma cell strain LSDV-ORF132-11H1, and the application realizes high-efficiency soluble expression of LSDV-ORF132 based on a prokaryotic expression system. After mice are immunized by taking LSDV-ORF132 as an immunogen, a LSDV positive hybridoma cell strain LSDV-ORF132-11H1 capable of stably secreting antibodies is successfully prepared and screened after cell fusion. The ELISA titer of the antibody obtained after the monoclonal antibody secreted by the cell strain is purified is 1:64000, the heavy chain of the antibody is an IgGa2 subtype, and the light chain is a Kappa chain. Based on the antibody, high-efficiency detection of bovine nodular dermatopathy virus ORF132 protein can be realized.

The utility model discloses an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit, which belongs to the technical field of biological detection equipment and comprises a microwell plate, a box body and a top cover, the box body and the top cover are matched with each other, an inner box is movably connected in the box body, a buffer component is arranged between the box body and the inner box, and a partition plate is fixedly connected in the middle of the inner box. A supporting net for placing the micro-pore plate is fixedly connected into the inner box above the partition plate, and a vibration assembly is fixedly mounted at the bottom of the inner box. According to the ELISA detection kit, the supporting net is used for supporting the microwell plate, the top cover is matched with the pressing block and the elastic assembly to fix the microwell plate, then the vibration assembly is matched to vibrate the inner box and the microwell plate, waste liquid passes through the supporting net, and the inner box is matched with the partition plate to temporarily store the waste liquid. The problem that a worker needs to hold the kit to move back and forth among the placing unit, the vibration unit and the waste liquid cylinder, and time and labor are wasted is solved, and the detection efficiency is improved.