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Microarray-ELISA detecting reagent kit for detecting autoimmunity disease relevant antibody spectrum

An enzyme-linked immunoassay detection and kit technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation of detection kits, single detection indicators, and high detection costs, and achieve good sensitivity and specificity. The effect of high operation requirements and simple operation

Inactive Publication Date: 2007-10-31
BEIJING BGI GBI BIOTECH +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Using the ENA antibody spectrum microarray-enzyme-linked immunoassay kit to detect systemic autoimmune diseases successfully overcomes the defects of the existing detection kits such as cumbersome operation, single detection index, and high detection cost. It is cheap, simple, fast, accurate, Multiple detection and other advantages

Method used

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  • Microarray-ELISA detecting reagent kit for detecting autoimmunity disease relevant antibody spectrum
  • Microarray-ELISA detecting reagent kit for detecting autoimmunity disease relevant antibody spectrum
  • Microarray-ELISA detecting reagent kit for detecting autoimmunity disease relevant antibody spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of ENA plate with immobilized protein antigens in ENA antibody profile microarray-enzyme immunoassay kit for detection of systemic autoimmune diseases

[0032] In order to detect whether the sample contains a drug selected from the group consisting of systemic lupus erythematosus (SLE), mixed connective tissue disease (MCTD), Sjogren's syndrome (SS), systemic scleroderma (PSS), polymyositis or dermatomyositis (PM / DM) and the characteristic antibody of systemic autoimmune disease of rheumatoid arthritis (RA), prepare the microtiter plate that is fixed against the antigen of following characteristic antibody: anti-Smith antibody, anti-RNP antibody ( anti-ribonucleoprotein antibody), anti-SSA antibody (anti-Sjögren syndrome antigen A antibody), anti-SSB antibody (anti-Sjogren syndrome antigen B antibody), anti-Scl-70 antibody, anti-Jo-1 antibody, and anti-Rib-P antibody (anti-ribosomal P protein antibody).

[0033] 1. Antigen Preparation

[0034] The specific...

Embodiment 2

[0040] Example 2 Determination of ENA Antibody Spectrum Microarray-ELISA Kit Using Autoimmune Disease Detection

[0041] 1. Add sample

[0042] Sample diluent (2.9g disodium hydrogen phosphate, 0.2965g sodium dihydrogen phosphate, 10g sodium chloride, 2.5g Casein, 5g aminopyridine, 100ml calf serum, 75ml glycerin, 5ml Tween-20, triton -1005ml, ProClin300 0.15ml, purified water added to 1000ml) add 50ul of the sample to be tested according to 1:50 dilution into each reaction well of the ELISA plate prepared as in Example 1 above, and put it into the incubation shaker, gear 3 React at 37°C for 60 minutes.

[0043] 2. Plate washing:

[0044] After the sample reaction is over, take the reaction plate out of the oscillator, put it into the corresponding position of the plate washer, adjust the "Number of Strips" button to the corresponding number of strips, and adjust the "Number of Times" button to 5 times. No soaking, no shaking, no pauses. After washing the plate, pat the pl...

Embodiment 3

[0050] Comparison of ENA antibody profile microarray-enzyme-linked immunoassay kit and existing technology

[0051]

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Abstract

This invention relates to one anti-extracting nuclear antigen spectrum array to ELISA test agent case for selecting for systematic lupus erythematosus, mixed connective tissue disease, Sjogren syndrome, systemic scleroderma, polymyositis and atrophic arthritis system self immune property antigen ENA spectrum micro array to enzyme immune agent case.

Description

field of invention [0001] The invention relates to an anti-extractable nuclear antigen (ENA) antibody profile microarray (Array)-enzyme-linked immunosorbent (ELISA) detection kit, especially for detecting systemic lupus erythematosus (SLE), mixed connective Systemic autoimmunity in histological disease (MCTD), Sjogren's syndrome (SS), systemic scleroderma (PSS), polymyositis or dermatomyositis (PM / DM), and rheumatoid arthritis (RA) ENA Antibody Spectrum Microarray-ELISA Kit for Characteristic Antibodies of Diseases. Background of the invention [0002] Systemic autoimmune diseases refer to a type of diffuse connective tissue diseases in rheumatic diseases, mainly including systemic lupus erythematosus (SLE), mixed connective tissue disease (MCTD), Sjogren's syndrome (SS), systemic sclerosis disease (PSS), polymyositis or dermatomyositis (PM / DM), and rheumatoid arthritis (RA). [0003] According to the current level of development of pathology and clinical medicine, the abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/545G01N33/535
Inventor 汪建杨玲牟峰张伟陈久龙郝志波姜范波何静云陈唯军沈东艳许东光顾志鹏沈远苏慧玲李黎万戈江方健秋文洁
Owner BEIJING BGI GBI BIOTECH
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