10 results about "Immobilized protein" patented technology

Provided herein are cell-based assays using immobilized recombinant proteins to measure immune regulation of therapeutic biologics. In particular, the disclosure relates to a method for assessing and / or determining immune response induced by a biologic molecule comprising: a) immobilizing a recombinant membrane protein or a fragment comprising the extracellular domain thereof on a support; b) contacting the immobilized protein or fragment with (i) a biologic molecule (such as an antibody); and (ii) an effector cell; and c) detecting the activation of the effector cell so as to assess and / or determine the immune response (such as ADCC, ADCP) induced by the biologic molecule.

Provided herein are cell-based assays for measuring the immunomodulation of therapeutic biologics using immobilized recombinant proteins. Specifically, the present disclosure relates to a method of assessing and / or determining a biomolecule-induced immune response, comprising: a) immobilizing a recombinant membrane protein or a fragment comprising an extracellular domain thereof on a support; b) contacting the immobilized protein or fragment with (i) a biomolecule, such as an antibody, and (ii) effector cells, and c) detecting activation of the effector cells to assess and / or determine an immune response induced by the biomolecule, such as ADCC, ADCP.

Disclosed are a method for preparing an immobilized enzyme or an immobilized protein and the immobilized enzyme or the immobilized protein. The method comprises: covalently binding SpyCatcher on a carrier; constructing a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal; and contacting the carrier covalently bound with SpyCatcher with the target enzyme or the target protein fused with SpyTag. The immobilized enzyme or the immobilized protein comprises a carrier, SpyCatcher covalently bound with the carrier, and a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal, which is covalently bound with the carrier through the SpyTag and the SpyCatcher. The present application also relates to a carrier covalently bound with SpyCatcher and the use thereof for selectively immobilizing a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal from a protein mixture.

A dried body of an immobilized enzyme or an immobilized protein having enzymatic activity thereof, which is an enzyme capable of enduring drying, wherein the enzyme or the protein having enzymatic activity thereof is a ketose 3-epimerase or a protein having ketose 3-epimerase activity, and the immobilized enzyme prior to drying or the immobilized protein having enzymatic activity thereof prior to drying is an immobilized enzyme or an immobilized protein having enzymatic activity thereof, in which a temperature (an optimum temperature) indicating maximum activity has further increased as compared with prior to immobilization. A manufacturing method including immobilizing the enzyme or the protein, manufacturing an immobilized enzyme or an immobilized protein having enzymatic activity of the enzyme, in which a temperature (an optimum temperature) indicating maximum activity has further increased as compared with prior to immobilization, and performing drying.

[Problem] To provide a ketose 3-epimerase or a protein having the same activity in a form in which an effect of the enzyme is maintained for a long period of time even under severe preservation conditions. [Solution] A dried body of an immobilized enzyme or an immobilized protein having enzymatic activity thereof, which is an enzyme capable of enduring drying, wherein the enzyme or the protein having enzymatic activity thereof is a ketose 3-epimerase or a protein having ketose 3-epimerase activity, and the immobilized enzyme prior to drying or the immobilized protein having enzymatic activity thereof prior to drying is an immobilized enzyme or an immobilized protein having enzymatic activity thereof, in which a temperature (an optimum temperature) indicating maximum activity has further increased as compared with prior to immobilization. A manufacturing method including immobilizing the enzyme or the protein, manufacturing an immobilized enzyme or an immobilized protein having enzymatic activity of the enzyme, in which a temperature (an optimum temperature) indicating maximum activity has further increased as compared with prior to immobilization, and performing drying. The enzyme or the protein is supported by a carrier which is an ion exchange resin, an organic polymer carrier, and / or an inorganic carrier, and is dried.

The present invention relates to a carbon-based polymeric material (also known as a porous carbon-based material) optionally complexed with at least one metal ion (preferably a metal cation); the invention also relates to a composition comprising a protein immobilized on the material, and a preparation method thereof. The invention further relates to the use of a composition comprising the immobilized protein, in particular in heterogeneous biocatalytic applications.

Provide is a novel system for immobilizing recombinantly produced proteins by entrapping them in crystals of co-expressed proteins that are capable of self-crystallization. Related compositions and as well as methods of making and using the immobilized proteins are also described.

Provided is a functionalized three-dimensional (3D) polymer scaffold which comprises immobilized proteins or protein fragments on its surface, which are capable of binding a target biological entity during affinity chromatography as well as to a method of manufacturing such 3D polymer scaffold.

This invention discloses a method for screening TNFR1-targeting active ingredients in Tremella fuciformis fermentation broth, belonging to the field of active ingredient screening. The method includes: preparing and immobilizing a recombinant TNFR1 extracellular domain protein with a His tag; optimizing the conditions for preparing Tremella fuciformis fermentation broth and removing polysaccharides; subjecting the fermentation broth to affinity incubation with the immobilized protein to specifically capture the binding components; washing to remove impurities and then competitively eluting with imidazole phosphate buffer; finally, separating the TNFR1-targeting active ingredients by the methanol-chloroform method. This method utilizes the specific affinity of the TNFR1 target to efficiently and accurately enrich immunologically active ingredients from the complex Tremella fuciformis fermentation broth. Experiments have shown that the obtained components can significantly promote the proliferation of macrophages and splenocytes, providing a new source of raw materials and technical support for the development of novel immune-enhancing products.

The invention discloses a preparation method of a novel sheet-shaped hollow porous chitin carrier and application of the novel sheet-shaped hollow porous chitin carrier in immobilized enzyme. A novel carrier MC-OA is created by utilizing shrimp meal source chitin (the mass ratio of choline chloride to oxalic acid is 1: 3), the novel carrier MC-OA is applied to immobilized enzyme, the chitin carrier has excellent loading performance on zymoprotein, the quantity of the immobilized protein is 3.39 times that of natural chitin, and the enzyme activity expression is improved to 1.91 times that of free enzyme BTL2. The novel carrier MC-OA provided by the invention has universality on loading of different sources of enzyme proteins, the protein loading capacity is improved, meanwhile, the activity increasing effect and stable catalytic performance of natural chitin on free enzyme can be reserved, and a good carrier source basis is provided for subsequent immobilized enzyme creation.