6 results about "Immobilized protein" patented technology

Provided herein are cell-based assays for measuring the immunomodulation of therapeutic biologics using immobilized recombinant proteins. Specifically, the present disclosure relates to a method of assessing and / or determining a biomolecule-induced immune response, comprising: a) immobilizing a recombinant membrane protein or a fragment comprising an extracellular domain thereof on a support; b) contacting the immobilized protein or fragment with (i) a biomolecule, such as an antibody, and (ii) effector cells, and c) detecting activation of the effector cells to assess and / or determine an immune response induced by the biomolecule, such as ADCC, ADCP.

Disclosed are a method for preparing an immobilized enzyme or an immobilized protein and the immobilized enzyme or the immobilized protein. The method comprises: covalently binding SpyCatcher on a carrier; constructing a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal; and contacting the carrier covalently bound with SpyCatcher with the target enzyme or the target protein fused with SpyTag. The immobilized enzyme or the immobilized protein comprises a carrier, SpyCatcher covalently bound with the carrier, and a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal, which is covalently bound with the carrier through the SpyTag and the SpyCatcher. The present application also relates to a carrier covalently bound with SpyCatcher and the use thereof for selectively immobilizing a target enzyme or a target protein fused with SpyTag at an N terminal or a C terminal from a protein mixture.

The present invention relates to a carbon-based polymeric material (also known as a porous carbon-based material) optionally complexed with at least one metal ion (preferably a metal cation); the invention also relates to a composition comprising a protein immobilized on the material, and a preparation method thereof. The invention further relates to the use of a composition comprising the immobilized protein, in particular in heterogeneous biocatalytic applications.

Provide is a novel system for immobilizing recombinantly produced proteins by entrapping them in crystals of co-expressed proteins that are capable of self-crystallization. Related compositions and as well as methods of making and using the immobilized proteins are also described.

Provided is a functionalized three-dimensional (3D) polymer scaffold which comprises immobilized proteins or protein fragments on its surface, which are capable of binding a target biological entity during affinity chromatography as well as to a method of manufacturing such 3D polymer scaffold.

This invention discloses a method for screening TNFR1-targeting active ingredients in Tremella fuciformis fermentation broth, belonging to the field of active ingredient screening. The method includes: preparing and immobilizing a recombinant TNFR1 extracellular domain protein with a His tag; optimizing the conditions for preparing Tremella fuciformis fermentation broth and removing polysaccharides; subjecting the fermentation broth to affinity incubation with the immobilized protein to specifically capture the binding components; washing to remove impurities and then competitively eluting with imidazole phosphate buffer; finally, separating the TNFR1-targeting active ingredients by the methanol-chloroform method. This method utilizes the specific affinity of the TNFR1 target to efficiently and accurately enrich immunologically active ingredients from the complex Tremella fuciformis fermentation broth. Experiments have shown that the obtained components can significantly promote the proliferation of macrophages and splenocytes, providing a new source of raw materials and technical support for the development of novel immune-enhancing products.