48results about How to "Exclude false positive results" patented technology

The present invention relates to oligonucleotides hybridizable with nucleic acids of pathogens causing sexually transmitted diseases, kits comprising them, and processes for amplifying and detecting viral nucleic acids using them. The present oligonucleotides completely overcome problems of false-negative and false-positive products in detection of pathogens causing sexually transmitted diseases using conventional primers and show dramatic workability in multiplex PCR, enabling to simultaneously detect pathogens causing sexually transmitted diseases in a single PCR reaction.

The invention discloses a stomach cancer serological detection and identification kit and a method. The stomach cancer serological detection and identification kit is prepared from a cel-miR-39-5p fragment, a primer, an exosome isolating reagent, an exosome miRNA (Micro Ribonucleic Acid) extracting reagent, a reverse transcription reagent and a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) reagent, wherein the primer comprises a cel-miR-39-5p internal reference primer, an hsa-miR-375 primer, an hsa-miR-590-5p primer, an hsa-miR-19b-3p primer, an hsa-miR-100-5p primer and an hsa-miR-16 primer. When any results of detecting stomach cancer CA (Carbohydrate Antigen)19-9, CA24-2 and CEA (Carcino Embryonie Antigen) of a patient by using serum are positive, and the stomach cancer serological detection and identification kit provided by the invention is then used for carrying out aided detection, false positive results of serological detection can be effectively removed, so that huge mental stress and property loss brought to the patient can be avoided; meanwhile, aiming at the situation that when the results of detecting the stomach cancer CA19-9, CA24-2 and CEA of the patient by using the sera are negative, and the stomach cancer serological detection and identification kit provided by the invention is then used for carrying out the aided detection,, the false negative results of the serological detection can be effectively found, so that the life of the patient can be rescued in time.

The invention relates to a monoclonal antibody of anti-blue crab particle hemocyte 26.7kDa protein, and the monoclonal antibody is secreted by hybridoma cell of which the name is hybridoma cell strain Crab, the preservation number is CCTCC NO: C2012196, the preservation unit is China Typical Culture Preservation Center, and the preservation data is Jan 7th, 2013. The monoclonal antibody has a characteristic of being specifically combined with blue crab particle hemocyte 26.7kDa protein. The preparation method comprises the steps of: obtaining particle hemocyte by continuous density gradient centrifugation of Percoll medium; purifying the particle hemocyte 26.7kDa protein as antigen to immunize mice by electrophoresis rubber cutting and recycling; preparing hybridoma cells by a cell fusion method; screening out the monoclonal antibody of the anti-blue crab particle hemocyte 26.7kDa protein by an immunological detection method. The monoclonal antibody disclosed by the invention provides an important tool for researching functions of particle hemocyte in nonspecific immune response of blue crab and in other physiological function aspects.

The invention discloses a method for screening and characterizing a metabolite-protein interaction system based on metabolomics and a structural mass spectrometric technique; a heterologously expressed tagged target protein is immobilized through an affinity immobilization method, then, the target protein is incubated with a metabolite extract, and finally, after being separated, denatured and eluted, the target protein-metabolite complex is subjected to non-targeted metabolomics analysis, and the complex is compared with a control group (an affinity vector without immobilized protein) so as to screen a metabolite with potential interaction with the target protein. Afterwards, the potential interaction system is directly verified using structural mass spectrometry. The binding method enables high-throughput screening of metabolites with potential interaction with the target protein, and also, direct characterization of the structural mass spectrometry can be used to screen out false positive interaction results that are probably introduced during indirect characterization. The method is a reliable high-throughput research tool for protein-metabolite interactions.

The invention discloses a kit for bisulfite conversion of free DNA. The kit comprises a bisulfite solution, a protective solution, washing liquor A, a magnetic bead suspension, washing liquor B, washing liquor C and eluate, wherein the protective solution is prepared from the following ingredients: 80% to 85% of tetrahydrofuran furfuryl alcohol, 10% to 15% of D-alpha tocopherol cetomacrogol 1000 succinate, 0.2% to 0.3% of tetrahydroglycosyl acetate, 0.05% to 0.1% of ethoxychrysoidine and the balance of water. The invention further provides a method for bisulfite conversion and DNA purificationof the free DNA by using the kit. According to the kit, a sulfite conversion method is subjected to great technological improvement, so that ctDNA conversion can be completed rapidly (40 to 60 minutes) under mild conditions (70 DEG C to 80 DEG C), pH of a system can be stabilized, ctDNA degradation is prevented, high-conversion-ratio, high-quality and high-purity free DNA can be rapidly obtained,and full automated operation of methylation researches is facilitated.

Ligation assays for detecting and profiling expression products at transcriptome scale.

The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8, or a part or the entire sequence of a sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8, wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene; a primer or a probe for the detection of M. intracellulare, which comprises the aforementioned oligonucleotide; and a method for detection of M. intracellulare using the aforementioned primer and/or the probe.

According to the detection method of the present invention, any false-positive result in diagnosis can be eliminated and detection or diagnosis of M. intracellulare can be carried out with higher accuracy, more preciseness, and more specifically compared to a conventional diagnostic method employing a cell culture assay or a PCR assay. The method also enables to quantify a microbial cell.

The invention discloses a multiple cross isothermal amplification method implemented by the aid of Antarctic thermal sensitive uracil-DNA (deoxyribonucleic acid)-glycosylase (AUDG) coupled with self-avoiding molecular recognition systems (SAMRS). The multiple cross isothermal amplification method includes carrying out SAMRS modification on four basic groups from a second basic group to a fifth basic group from the bottom of a 3' end of each primer during multiple cross displacement amplification; labeling haptens at 5' ends of amplification primers C1 or C2; leading the AUDG, deoxidized uracil and biotinylated deoxidized cytosine into amplification systems; detecting amplification products by the aid of coupled macromolecule nano biosensing on the basis of multiple cross displacement amplification technologies. The multiple cross isothermal amplification method has the advantages that the amplification products of IS6110 specific sequences of mycobacterium tuberculosis complexes can be pertinently visually detected by macromolecule nano biosensors; the multiple cross isothermal amplification method is convenient, speedy, sensitive and specific and is suitable for detecting diversified nucleotide fragments.

The present invention relates to a rapid assaying card to detect organic phosphor and carbamate pesticide residues in various agricultural products. Organic phosphor and carbamate pesticide pesticides are commonly used in China as main residual pesticide in vegetables, fruit and other agricultural products. In order to meet demand for rapid circulation of agricultural products, the rapid assaying card as a product to rapidly, easily and economically detect organic phosphor and carbamate pesticide residues emerges. However, rapid assaying cards current sold in the market suffer from serious false positive while detecting white turnip, garlic and so on, thus restricting applicable scope of the rapid assaying card. The rapid assaying card provided by the present invention to detect organic phosphor and carbamate pesticide residue in various agricultural products comprises 1% dilute sulphuric acid pre-processing reagent, 0.2% oxydol detection result confirmation reagent, and rapid assaying card composed of ferment tablets and substrates and 0.1 mol /L pH 7.5 phosphate buffers.

The invention discloses a real-time direct analysis method for rapidly determining free formaldehyde in water-soaked products. The method comprises the following steps: enabling 2,4-dinitrophenylhydrazone as a reaction reagent to react with formaldehyde, taking an appropriate amount of reaction liquid to be dripped on a screen so as to be naturally dried in air to obtain a product, placing the product between a real-time direct analysis mass spectrum ion source gas outlet and a triple quadrupole mass spectrometer ion inlet, and determining, wherein DART ion source conditions are as follows: a negative ion mode is adopted and an ion source temperature is 300 DEG C, and triple quadrupole mass spectrometer conditions are as follows: the mass analyzer voltage is 3000V, and the mass-to-charge ratio of parent ions to quantitative ions to qualitative ions is 209 to 151 to 133 in a second-grade scanning mode; and by comparing second-grade mass spectrograms of a sample and a control sample, analyzing the free formaldehyde in the water-soaked products and quantifying by adopting an external standard method. The real-time direct analysis method provided by the invention has the advantages that the complex pretreatment and chromatographic separation process is not needed, the detection limit is up to 1ppm, and the detection method is sensitive, simple and convenient, and can be used for rapidly detecting the free formaldehyde in the water-soaked products.

The invention belongs to the technical field of analysis, and particularly relates to a cell wax block preparation kit and a preparation method thereof. The cell wax block preparation kit comprises a reagent A and a reagent B; and the reagent A is an aqueous solution prepared from any one of polyanionic celluloses, polyanionic cellulose sodium, polyanionic cellulose potassium, carboxymethylcellulose, sodium carboxymethylcellulose and potassium carboxymethylcellulose, and the reagent B is an organic solvent. The reagent provided by the invention is used for producing a cell wax block, so that fewer samples such as tissue fluid and the like are needed, the success rate is high, no adverse effect on the cell is produced, the loss of the cell is reduced, more cells are maintained, the detection accuracy is improved, the application value for preparing the cell wax block for cell needle aspirations, cutting needle biopsy, body fluid and residual deposits and various micro fragments prepared by other cytology clinically is high, and the daily increasing requirements on the scientific research and clinical pathological diagnosis can be met.

A TNF-α binding polypeptide is provided, which is related to a domain of staphylococcal protein A (SPA) in that the sequence of the polypeptide corresponds to the sequence of the SPA domain having 1 to about 20 substitution mutations. Nucleic acid encoding the polypeptide, expression vector comprising the nucleic acid, and host cell comprising the expression vector are also provided. Also provided are methods comprising a step of affinity separation or detection, in which step a polypeptide according to the invention is used. Such methods may be used for reducing the content of TNF-α in a body fluid.

The invention discloses two detection targets 3283/3316 for Yersinia enterocolitica and detection primers for the Yersinia enterocolitica. Sequences of the detection targets are shown in SEQ ID NO: 1and SEQ ID NO: 2. The detection targets for the Yersinia enterocolitica, disclosed by the invention, can be applied to rapid detection on the Yersinia enterocolitica in samples of foods and the like.The special detection primers disclosed by the invention particularly comprise a 3283 marker forward primer 3283-F, a 3283 marker reverse primer 3283-R, a 3316 marker forward primer 3316-F and a 3316marker reverse primer 3316-R. Through PCR amplification and agarose gel electrophoresis detection, a detection result can be obtained from an electrophoresis product result. The specific detection molecular markers and the rapid detection method can be used for effectively detecting the Yersinia enterocolitica in the samples of the foods and the like, have the advantages of rapidness, high efficiency, simplicity in operation and the like and can be applied to the fields of foods, inspection and quarantine and the like.

The invention discloses a fluorescent microsphere-colloidal gold double-color-development qualitative and quantitative immunochromatographic test strip for detecting ractopamine, and a preparation method of the test strip. A bottom plate is lapped and pasted with filter paper, a sample pad, a glass fiber mat sprayed with a fluorescent microsphere-ractopamine monoclonal antibody complex and a colloidal gold-ractopamine monoclonal antibody complex, a nitrocellulose membrane and water absorption paper in sequence; and the nitrocellulose membrane is coated with a ractopamine coupled antigen as a detection line and is coated with an anti-mouse antibody as a quality control line. When the color development degree of the detection line is lower than that of the quality control line, the content of the ractopamine in a sample is greater than 0.5ng/ml, and the test strip is qualitatively judged to be positive. The test strip qualitatively judged to be positive is directly inserted into a fluorescence reader, and the reader performs numeralization on a fluorescent signal of a fluorescent microsphere color development system so as to realize quantitative detection. The test strip is mainly used for qualitative and quantitative detection of the ractopamine in food safety detection.

The present invention relates to an oligonucleotide, comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium intracellulare (M. intracellulare) gene; a primer or a probe for the detection of M. intracellulare which comprises said oligonucleoride; and a method for detection of M. intracellulare using said primer and/or the probe.

The invention relates to a colloidal gold card for detecting pesticide residues as well as a preparation method and application of the colloidal gold card. The colloidal gold card comprises a bottom plate, and a sample pad, a gold combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially lapped on the bottom plate, wherein the nitrocellulose membrane is provided with a detection line coated with a pesticide antigen and a quality control line coated with a second antibody. The gold conjugate pad contains a colloidal gold labeled pesticide antibody, cane sugar, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose and polyethylene glycol. In the colloidal gold card, a gold conjugate pad contains cane sugar, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose and polyethylene glycol; the colloidal gold card can effectively protect antigens and antibodies, eliminates the influence of tea polyphenols, flavonoid substances, pigments, minerals and the like in tea water on color development, and is improved in the anti-interference performance.

The invention relates to the field of single cell detection and analysis, and discloses a method for single cell detection and analysis. According to the method, single cell separation and cell lysis are carried out on a sample by a one-step method by utilizing a digital PCR instrument, and a target sequence in the sample is detected and analyzed, so that the application range of the digital PCR instrument is expanded, and the digital PCR instrument can be used for detecting micro cells; and meanwhile, a target gene in each single cell in the sample can be accurately quantified, the detection of the expression level of the target gene can be realized, and the method can also be used for verifying a single cell sequencing result.

The invention relates to a novel antidepressant compound and pharmaceutically acceptable salts thereof. The compound is 4'-bromo-7-isoamylene oxo-2,3-flavanone which is represented by a formula (I) as shown in the description. A synthetic method of the compound comprises: by taking a compound represented by a formula (II) shown in the description as a starting material, carrying out a reaction with p-bromobenzaldehyde; and recrystallizing the obtained product to obtain the compound represented by the formula (I). From the acute toxicity test result of a mouse, the compound is very low in general toxicity and is an effective antidepressant drug.

The invention provides a method for intelligently detecting BECT spike waves based on multichannel electroencephalograms. The method comprises the following steps: (1) collecting the electroencephalograms (EEG), and establishing an experimental database; (2) preprocessing data: carrying out band-pass filtering on collected EEG data, so as to obtain a standard EEG signal; (3) carrying out candidatespike wave detection, carrying out self-adaptive template matching by utilizing a screened class-center as a new template, and adding all matching results together so as to obtain candidate spike waves; (4) eliminating false-detection spike waves: determining two relevant BP channels of each candidate spike wave according to a candidate detection result of an AV channel, and removing the candidate spike waves without an "eye-for-eye" phenomenon on the two BP channels; (5) extracting characteristics of the spike waves: after the false-detection spike waves are eliminated, calculating 10 characteristics of each channel; and (6) carrying out random forest classification: training a random forest classification model by taking the extracted characteristics of the spike waves as a characteristic vector, and inputting the characteristics of the spike waves of to-be-analyzed electroencephalograms into the random forest classification model, so as to obtain a detection result of the BECT spike waves.

The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence depicted in SEQ ID NO: 1, 2, 3 or 4, or a part or the entire sequence of a nucleotide sequence complementary to the nucleotide sequence, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium kansasii; a primer and a probe for use in the detection of Mycobacterium kansasii comprising the oligonucleotide; and a method for detecting Mycobacterium kansasii using the primer and/or probe.

The method for detecting Mycobacterium kansasii enables the detection of M. kansasii more rapidly and with higher accuracy compared with a conventional bacterium identification method performed by culture examination on a bacterium. Further, the method can exclude any false positive result for the diagnosis and can also detect and diagnose M. kansasii with higher accuracy compared with a diagnosis method performed by PCR using a conventional primer and/or probe. Still further, the method can quantify a M. kansasii cell.

The invention relates to application of MxA and SAA or a binding antibody or fragment thereof in preparation of a diagnostic kit for discriminating viral influenza and non-influenza of children. An immunofluorescence method is adopted to monitor influenza-like symptom patients including influenza A, influenza B, influenza C, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, adenovirus and respiratory syncytial virus infection. The kit can effectively eliminate false positive results in diagnosis, has high specificity and sensitivity, is simple to operate, has been clinically verified, is suitable for further clinical popularization, not only improves the diagnosis sensitivity of children influenza, but also detects non-specific inflammatory factors SAA while detecting MxA. Also, the kit has the advantage that bacterial infection or virus infection can be distinguished.

The invention discloses a loop-mediated isothermal amplification method implemented by combining antarctic thermal sensitive uracil deoxyribonucleic acid glycosylase (AUDG) with a self-avoiding molecule recognition system (SAMRS). According to the method, in a process of loop-mediated isothermal amplification, SAMRS modification is performed on four bases from the reciprocal second base to the reciprocal fifth base at the 3' end of a primer, and the 5' end of a primer LF is labeled with a hapten; the AUDG, deoxyuridine and biotinylated deoxycytosine are introduced into an amplification system;the loop-mediated isothermal amplification method is based on a loop-mediated isothermal amplification technology and is used for detecting amplification products in combination of a polymer nanometer biosensor. The method aims at the fact that the amplification products of an IS6110 specific sequence of a mycobacterium tuberculosis complex group can be visually detected by the polymer nanometerbiosensor. The method is convenient and fast, rapid, sensitive and specific, and is suitable for detecting various nucleotide fragments.

The invention relates to the field of manufacturing of a sensor, particularly relates to a quality sensor for gene detection, and provides a quality sensor for gene detection. The quality sensor comprises a quality sensor body. The quality sensor is characterized in that a polyethylene glycol monolayer is arranged on the surface of a non-detected sensitive position of the quality sensor; a thiol biotin monolayer is arranged on the surface of the detected sensitive position of the quality sensor; a streptavidin layer is grafted on the thiol biotin monolayer. The quality sensor provided by the invention has the main advantages of real-time online detection, accurate result, fast detection speed, economic cost and good expansibility.