Kit for bisulfite conversion of free DNA
A bisulfite and kit technology, which is applied in the field of free DNA bisulfite conversion kits, can solve the problems of DNA methylation information loss, failure, and DNA degradation, and achieve the effect of real-time monitoring efficiency
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Embodiment 1
[0037] Embodiment 1 is used for the kit of free DNA bisulfite conversion
[0038] The kit includes the following components: bisulfite solution, protection solution, washing solution A, magnetic bead suspension, washing solution B, washing solution C and eluent.
[0039] The bisulfite solution is composed of the following components, each component is by weight percentage: 50% ammonium bisulfite, 4% sodium bisulfite, 2% ammonium sulfite monohydrate, 0.8% TritionX-100, The balance is water, and the pH value is between 5.3 and 5.6.
[0040] The protective solution is composed of the following components, each component is calculated by weight percentage: tetrahydrofurfuryl alcohol 83%, D-alpha-tocopheryl acid polyethylene glycol 1000 succinic acid (TPGS) 12%, tetrahydroglycol acetate 0.25% %, 0.08% of ethoxylated yellow chil, and the balance is water.
[0041] The lotion A is composed of the following components, each component is calculated by weight percentage: 22% of guanid...
Embodiment 2
[0098] Embodiment 2 is used for the kit of free DNA bisulfite conversion
[0099] The kit includes the following components: bisulfite solution, protection solution, washing solution A, magnetic bead suspension, washing solution B, washing solution C and eluent.
[0100] The bisulfite solution is composed of the following components, each component is calculated by weight percentage: 50% ammonium bisulfite, 2% sodium bisulfite, 3% ammonium sulfite monohydrate, 0.5% TritionX-100, The balance is water, and the pH value is between 5.3 and 5.6.
[0101] The protective solution is composed of the following components, each component is calculated by weight percentage: tetrahydrofurfuryl alcohol 80%, D-alpha-tocopheryl acid polyethylene glycol 1000 succinic acid (TPGS) 15%, tetrahydroglycol acetate 0.2% %, 0.1% of ethoxylated yellow chil, and the balance is water.
[0102] The lotion A is composed of the following components, each component is calculated by weight percentage: guan...
Embodiment 3
[0107] Embodiment 3 is used for the test kit of free DNA bisulfite conversion
[0108] The kit includes the following components: bisulfite solution, protection solution, washing solution A, magnetic bead suspension, washing solution B, washing solution C and eluent.
[0109] The bisulfite solution is composed of the following components, each component is by weight percentage: 50% ammonium bisulfite, 6% sodium bisulfite, 1% ammonium sulfite monohydrate, 1% TritionX-100, The balance is water, and the pH value is between 5.3 and 5.6.
[0110] The protection solution is composed of the following components, each component is calculated by weight percentage: tetrahydrofurfuryl alcohol 85%, D-alpha-tocopheryl acid polyethylene glycol 1000 succinic acid (TPGS) 10%, tetrahydroglycol acetate 0.3% %, 0.05% of ethoxylated yellow chil, and the balance is water.
[0111] The lotion A is composed of the following components, each component is calculated by weight percentage: guanidine i...
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