13 results about "DNA methyltransferase" patented technology

The application belongs to the field of chemical medicines, and particularly relates to a quinoline compound, a preparation method and use thereof and a pharmaceutical composition thereof. The application provides a quinoline compound shown in formula I, which has excellent DNA methyltransferase 1 (DNMT1) inhibitory activity and good selectivity, can be used as a novel DNMT1 inhibitor, and provides a new option for drug development and application of diseases driven by DNMT1. Formula I.

The application discloses a kind of small molecule compound combination and the method for preparing vascular endothelial cell using the small molecule compound combination induced differentiation cell.The method is directed to induce differentiated cell, and finally prepare vascular endothelial cell;The directional induction includes inhibiting the activity of lysine deacetylase, inhibiting the signal path of TGF-β, inhibiting the activity of DNA methyltransferase (DNMT), activating the signal path of WNT / β-catenin and activating the signal path of cAMP.The present application can be converted into vascular endothelial cell by small molecule compound combination induction, and each step can realize accurate quality control, which is convenient for standardized operation and large-scale production.The method provided by the present application has wide donor sources, and the patient himself can be used as a donor, so that the vascular endothelial cells required for basic research, clinical treatment or tissue engineering production can be obtained in a short time.

The disclosure provides means, methods, and compositions of preventing, reducing, and treating kidney failure through the administration of fibroblasts, modified fibroblasts, and / or products derived from fibroblasts. The disclosure may also concern administration of fibroblasts prior to, concurrent with, or subsequent to administration of a nephrotoxic agent results in production of renal function. In some embodiments, fibroblasts are enhanced for augmentation of nephron-regenerative properties through culture under means including hypoxia, histone deacetylase inhibitor treatment, oxytocin, and / or DNA methyltransferase inhibitors.

Pharmaceutical compositions and compounds comprising a phosphorothioated CpG oligodeoxynucleotide linked to a DNA oligonucleotide that is hybridized an RNA aptamer are useful in methods of treating cancer (such as leukemia) and methods of inhibiting DNA methyltransferase. In embodiments, the RNA aptamer binds to an intracellular target such as DNMT1, NF-kB, RUNX1, MYC, MYB, ETS, PAX5, MDM2, F0XM1, PU.l, STAT3, STATS. STAT6, FAD, ATP5B, or beta-catenin.

The invention belongs to the field of plant molecular biology, and relates to application and a method of a vietnamese sophora root DNA methylase gene in improving cadmium stress tolerance of plants. The invention provides a DNA methylase gene StCMT1 from vietnamese sophora root, the nucleotide sequence of the DNA methylase gene StCMT1 is shown as SEQ ID No.2, and the amino acid sequence of an encoded protein is shown as SEQ ID No.1. According to the invention, by combining transcriptome screening with qRT-PCR verification, StCMT1 is determined as a key candidate gene for continuous inducible expression under cadmium stress; functional verification is carried out in tobacco, it is proved that overexpression of StCMT1 can enhance cadmium resistance under the stress conditions of 100 [mu] M and 200 [mu] M CdCl2, and definite repeatability and application value are achieved. The method can be used for constructing cadmium-resistant plant materials and cultivating cadmium-resistant varieties, and a new molecular target and technical scheme are provided for safe production of cadmium-contaminated soil and quality improvement of medicinal plants.

The invention discloses a preparation method of a spherical DNA nano machine for detecting O6-methylguanine-DNA methyltransferase (MGMT) and application of the spherical DNA nano machine in detection of the expression level of tumor cells MGMT. The method comprises the following steps: firstly, designing a DNA walker with MGMT recognition and signal amplification functions, and fixing the DNA walker on the surface of a gold nanosphere through sulfydryl modification to construct a spherical DNA nanomachine; when the MGMT interacts with a DNA Walker chain, a methylated basic group G on DNA enzyme can be repaired, so that the activity of the DNA enzyme is recovered, an enzyme digestion reaction is initiated, a Track chain marked with a fluorophore is broken and released, a fluorescence signal change is generated, and high-sensitivity detection of the MGMT is realized. The method can effectively detect the MGMT protein in a low concentration range, and has a good biomedical application prospect.

The disclosure provides means, methods, and compositions of preventing, reducing, and treating kidney failure through the administration of fibroblasts, modified fibroblasts, and / or products derived from fibroblasts. The disclosure may also concern administration of fibroblasts prior to, concurrent with, or subsequent to administration of a nephrotoxic agent results in production of renal function. In some embodiments, fibroblasts are enhanced for augmentation of nephron-regenerative properties through culture under means including hypoxia, histone deacetylase inhibitor treatment, oxytocin, and / or DNA methyltransferase inhibitors.

This invention belongs to the field of animal cell culture technology, specifically relating to a cytokine-free human pluripotent stem cell culture medium. The technical problem this invention aims to solve is that the cytokines used in existing pluripotent stem cell culture media are expensive and easily degraded during the culture process. This invention provides a cytokine-free human pluripotent stem cell culture medium, comprising a basal medium, a spleen tyrosine kinase SYK inhibitor, and a non-covalent inhibitor of DNA methyltransferase Dnmt1. This culture medium has a simple and well-defined composition; two small chemical molecules (R406, GSK3484862) replace traditional cytokines (FGF2, TGFB), enabling effective expansion of pluripotent stem cells in vitro and maintaining their differentiation capacity.

The application discloses application of DNMT3B as a marker in preparation of a severe EV71 infection diagnosis product, and belongs to the biomedical field. It is found by the application that the nucleocytolysis degree and ubiquitination level of DNA methyltransferase DNMT3B can be used for diagnosing children with severe EV71 infection. EV71 infection promotes partial transport of DNMT3B in the cell nucleus to the cytoplasm, and promotes K63 ubiquitination modification of DNMT3B by combining the 3C and 3D proteins with DNMT3B, so that DNMT3B is finally degraded by a proteasome. The reagent for detecting the nucleocytolysis degree of DNMT3B and / or the reagent for detecting the ubiquitination level of DNMT3B can be used for preparing a severe EV71 infection diagnosis product or a severe EV71 infection prognosis diagnosis product, so as to realize diagnosis of severe EV71 infection or prognosis thereof.

Malignant glioma has a poor prognosis, despite current therapeutic modalities. Standard of care therapy consists of surgical resection, fractionated radiotherapy concurrently administered with temozolomide (TMZ), a DNA-alkylating chemotherapeutic agent, followed by adjuvant cycles of TMZ. O6-methylguanine DNA methyltransferase (MGMT) is a DNA repair enzyme which removes alkylated lesions from tumor DNA, thereby promoting chemoresistance. MGMT promoter methylation is a known predictor of temozolomide responsiveness; patients with unmethylated MGMT have a poorer prognosis. We employed deactivated Cas9-CRISPR technology to effectively target methylation in the vicinity of the MGMT methylation-specific polymerase chain reaction (MSP) region, as mediated by the catalytic domain of DNA methyltransferase DNMT3A. In doing so, we discovered that the selected dCas9 / CRISPR-mediated methylation of certain regions of MGMT gene sequences can be used to enhance chemosensitivity in malignant glioma cells.

The application belongs to the technical field of medicines, and particularly relates to a compound with inhibitory activity on DNA methyltransferase 1 (DNMT1) and a preparation method and application thereof. In the application, a DNMT1 small-molecule inhibitor with a 2-isoindole-3-carbazolyl propionic acid skeleton is found and prepared, and is expected to be applied to the treatment of diseases related to DNA hypermethylation, especially tumor treatment.

Provided are fusion proteins and DNA-targeting systems for targeted transcriptional repression comprising an effector domain comprising engineered DNA methyltransferase 3 proteins or portions thereof, such as DNA methyltransferase 3L proteins or portions thereof. In some aspects, the compositions and methods provided herein facilitate targeted transcriptional repression by targeting the effector domain to a target site, such as a target site for a target gene. Also provided are methods and uses related to the provided fusion proteins, for example in connection with therapeutic applications.

The invention discloses a method for improving cadmium resistance of gynostemma pentaphyllum and reducing cadmium accumulation through 5-azacytidine and application, and belongs to the technical field of plant cultivation and epigenetic regulation. In order to solve the problems that the growth of the gynostemma pentaphylla is inhibited and the cadmium content of the overground part exceeds the standard due to cadmium pollution, 5-azacytidine and CdCl2 are applied in a water culture stage for composite stress treatment, the 5-azacytidine is used for inhibiting the activity of DNA methyltransferase and reducing the methylation level of genomic DNA, so that the expression of a cadmium transport gene is regulated and controlled, and the cadmium content of the overground part is reduced. Transferring of cadmium to the overground part is inhibited, and enrichment of cadmium in the root system is promoted; meanwhile, an antioxidant enzyme system is activated, oxidative damage is relieved, and saponin synthesis is promoted. The method is mainly used for cultivating gynostemma pentaphyllum in a cadmium-polluted environment, so that the cadmium resistance of the gynostemma pentaphyllum is enhanced, the cadmium content of medicinal parts is reduced, and the safety and quality of medicinal materials are guaranteed.