477 results about "Signal Pathways" patented technology

The invention relates to the technical field of biology, in particular to application of a rice OsPBL8 gene in enhancing resistance of rice to magnaporthe oryzae. The rice OsPBL8 gene is a rice endogenous gene participating in the processes of plant growth and development, abiotic stress response, hormone signal channel regulation and control and the like. The invention provides application of the OsPBL8 gene in the field of disease resistance, the OsPBL8 gene is overexpressed through gene editing, and the expression quantity of receptor-like cytoplasm kinase OsPBL8 in plants is increased, so that a plurality of signal channels are regulated and controlled, and the germ resistance of the plants is synergistically improved. By adjusting key hub nodes of plant targeted immune signal transduction, more lasting broad-spectrum disease resistance can be provided, good universality is achieved, disease resistance and growth can be balanced, good practicability is achieved, and improvement of disease resistance of various rice varieties is facilitated.

The invention discloses a chimera for degrading a CARD structural domain protein aggregate and application of the chimera. The chimera is prepared from light-operated targeting protein and light-operated degradation protein, the light-operated targeting protein is sequentially connected by an MAVS CARD structural domain, a flexible connecting peptide and a photosensitive protein pMag; the light-controlled degradation protein is sequentially connected by a photosensitive protein nMag, a flexible connecting peptide and an RING structural domain of TRIM21. According to the application, a light-operated activated CARD-RING chimera is constructed, and when the CARTAC generates toxic and side effects or in cells with RIG-I / MDA5 signal channels abnormally activated, the activity of the CARTAC can be effectively controlled or autoimmune diseases caused by RIG-I / MDA5 abnormity can be inhibited.

The invention relates to the technical field of strategy optimization, in particular to an ASIC (Application Specific Integrated Circuit) layout optimization method based on hybrid shaping programming, which comprises the following steps of: analyzing a circuit netlist, and establishing a signal path physical model containing static topological connectivity; a candidate layout scheme is generated based on the model, and layout state data describing the state of the candidate layout scheme is generated through physical effect simulation; based on the layout state data, calculating a signal processing performance index and a wiring cost value, and generating a group of dynamic shaping constraints for guiding subsequent layout according to the index and the cost value, the dynamic shaping constraints being quantitative adjustment of a path congestion perception graph in a signal path physical model; and updating the model through the constraint, evaluating the stability of a Pareto optimal boundary formed by the performance index and the cost value so as to judge convergence, and finally outputting a layout scheme associated with a stable state. According to the method, dynamic shaping constraints are generated through a strategy optimization algorithm, and global collaborative optimization of multiple targets such as time sequence and wiring performance is achieved.

The invention discloses a DCS system IO module channel testing device, and relates to the technical field of industrial automation equipment detection. The device comprises an IO module point inspection instrument and an upper computer, the IO module point inspection instrument is in communication connection with the upper computer, a plurality of sets of independent test modules capable of running in parallel are arranged in the IO module point inspection instrument, and each test module comprises a controller, an excitation source circuit, a signal acquisition circuit and a matrix switch circuit and corresponds to one slot. And the test module establishes a signal path with the IO module connected to the slot through the matrix switch circuit. Wherein a controller in the test module can automatically identify the type of the IO module to be tested, dynamically configure an excitation source circuit and a signal acquisition circuit which are matched with the IO module to be tested, and realize automatic and high-precision detection of various types of IO modules. According to the device, the point inspection efficiency of the IO module of the DCS system can be greatly improved by integrating a multi-channel signal output function and a signal detection function.

The invention discloses a non-reflection band-pass filter chip based on a novel topological structure, which is characterized by comprising a dielectric substrate, a filter circuit arranged on the dielectric substrate, an input port and an output port, a topological structure of the filter circuit is in bilateral symmetry, a main signal path of the filter circuit is of a five-order band-pass filter structure, and the filter circuit comprises a first series resonator, a third series resonator and another first series resonator which are sequentially connected between an input port and an output port in series; one ends of the two second parallel resonators are respectively connected between the first first series resonator and the third series resonator and between the third series resonator and the second first series resonator, and the other ends of the two second parallel resonators are grounded; the two ends of each first series resonator are connected with an absorption branch in parallel, each absorption branch is formed by sequentially connecting a fourth parallel resonator, a resistor and a fifth parallel resonator in series, and out-of-band energy absorption is achieved through the two branches of the same structure. An absorption circuit and a main filter circuit are organically fused, the problem of out-of-band reflection of a traditional filter is solved, and meanwhile the filter has the advantages of being compact in structure, small in size and high in integration degree.

The application provides application of a TT2-SCT1 / SCT2-WR2 regulation channel in plant heat stress tolerance. The application first discloses a new signal channel involved in plant heat tolerance: a TT2-SCT1 / SCT2-WR2 signal channel; wherein, TT2 is a negative regulation factor for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, SCT1 / SCT2 are also negative regulation factors for regulating the heat tolerance of plants or protecting (maintaining) the yield of plants in Gramineae under high temperature, and WR2 is a positive regulation factor for playing a role, which is beneficial to maintaining the homeostasis in plants. The application has important theoretical significance for genetic improvement of plant traits.

The invention provides a chicken PGC reporter gene cell strain for detecting the biological activity of FGF2 as well as a construction method and application of the chicken PGC reporter gene cell strain, and belongs to the technical field of biological activity detection. The cell strain is a recombinant cell which is stably integrated with a reporter gene expression cassette responding to an FGF signal channel in a chicken PGC genome; the expression cassette comprises a serum response element (SRE), a minimum promoter and a reporter gene (such as a luciferase gene). The construction method comprises the following steps: introducing a donor vector containing the elements into chicken PGC by utilizing a PiggyBac transposon system, and screening to obtain a stably expressed cell strain. The cell strain provided by the invention retains endogenous signal channel characteristics of chicken PGC, and can specifically and highly sensitively respond to FGF2 stimulation. The method can be used for biological activity quantitative determination and thermal stability evaluation of the FGF2 protein and the mutant thereof, quality control of culture medium components and screening of an FGF signal channel regulator.

The invention provides an anti-PD-1 antibody, a CAR-T cell, and a preparation method and application thereof. The anti-PD-1 antibody comprises LCDR-1-3 as shown in SEQ ID NO: 1-3 and HCDR-1-3 as shown in SEQ ID NO: 4-6, respectively. The CAR-T cell expresses a novel element for efficiently blocking the PD-1, and the element is a single-chain antibody for targeting the PD-1 in a cell membrane anchoring manner. And the chimeric antigen receptor and the cell membrane anchored anti-PD-1 scFv are connected by a 2A cleavage protein. The membrane anchor type anti-PD-1 scFv expressed by the CAR-T cell can almost completely block the expression of PD-1 on the surface of a T cell membrane, and further block a signal channel combined with PD-1 / PD-L1, so that the capability of T cell depletion caused by antagonism tumor of the CAR-T cell is enhanced, and the purpose of enhancing the anti-tumor effect of the CAR-T cell is achieved.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses a regulation mechanism research method of depleted precursor CD8T cells, and relates to the field of immunology and molecular biology. Comprising the following steps: detecting the expression level of the ARHGAP9 gene in a chronic virus infection model; constructing a T cell specific ARHGAP9 gene knockout animal model; the influence of ARHGAP9 deletion on the frequency, phenotype and function of the Tpex cell is analyzed; a single cell transcriptome sequencing technology reveals that ARHGAP9 regulates and controls a downstream signal channel of a Tpex cell. By constructing a chronic virus infection model, detecting the expression dynamic state of the ARHGAP9 gene, knocking out an animal model by utilizing T cell specificity ARHGAP9, and combining flow cytometry, qPCR, single cell transcriptome sequencing and other technologies, the invention discloses the effect of the ARHGAP9 as a novel immune checkpoint molecule, and provides a theoretical basis for developing Tpex cell targeting immunotherapy.

The present application relates to the technical field of medicine, in particular to the application of gansix triol A in the preparation of a drug for treating gastric cancer and / or non-small cell lung cancer, gansix triol A plays an anti-gastric cancer role by selectively inhibiting the proliferation of gastric cancer MGC-803 cells and inhibiting angiogenesis, the mechanism involves inhibiting the VEGF / VEGFR2 signal pathway and inducing cell apoptosis; the mechanism of gansix triol A in inhibiting the VEGF / VEGFR2 signal pathway is similar to that of bevacizumab; gansix triol A plays an anti-non-small cell lung cancer role by selectively inhibiting the proliferation of non-small cell lung cancer A549 cells, the mechanism involves inhibiting the MAPK / ERK signal pathway and inducing cell apoptosis. The present application finds that the cytotoxic activity of gansix triol A on MGC-803 cells and A549 cells is significantly stronger than that of tanshinone IIA; gansix triol A has an anti-tumor effect of "one drug with double targets, double inhibition" and "high efficiency and low toxicity".

This application provides a cable anti-theft device for construction sites, comprising: a transmitting module; and a receiving module, which, along with the transmitting module, is respectively disposed within cable end caps at both ends of the cable and connected to the same conductor within the cable via conductive connectors to form an electrical signal path. The receiving module includes a loop detection unit and an alarm unit for signal connection. The loop detection unit detects the continuity of the electrical signal path; the alarm unit triggers a first alarm upon interruption of the electrical signal path. This device, through the transmitting and receiving modules and the conductive connectors, forms an electrical signal path with the same conductor within the cable. The formation of this path is independent of whether the cable is energized; even if the cable is not energized, as long as the conductor is intact, the electrical signal path remains unobstructed. When the cable is cut, the conductor breaks, and the electrical signal path is interrupted. The loop detection unit detects this promptly, and the alarm unit issues an alarm, thus solving the problem of dependence on the cable being energized.

The invention provides an application of M1 as an affinity of human source PD-L1 and / or mouse source PD-L1, and relates to the technical field of biological medicine. The invention proves that M1 can be used as a PD-L1 affinity for human / mouse cross reaction, and has low nanomole affinity for two targets. In cell-based function detection, M1 has similar affinity with mPDL1 and hPD-L1 expressed on the surface of a cell, and signal transduction is inhibited through a PD-L1: PD1 axis at a low micromolar concentration. The M1 disclosed by the invention can be used as a non-antibody treatment method to damage a PD-L1: PD1 signal channel, and the M1 shows high tumor permeability compared with an antibody, and particularly has a remarkable advantage in tumors (such as pancreatic tumors) with high fiber hyperplasia; the M1 shows selective uptake in the tumor for expressing the PD-L1.

The present application provides a kind of debugging system and debugging method for mechanical and electrical engineering, the system includes oscillation generator, for sending oscillation signal, in the passage confirmation stage, it is arranged at the pre-installation position of the element to be detected;Field transceiver digital-analog circuit processing unit for receiving the oscillation signal sent by oscillation generator and carrying out signal type conversion, controller unit, including digital-analog circuit processing unit and signal oscillation generator unit;Digital-analog circuit processing unit, in the passage confirmation stage, for signal type conversion and output analog signal;Signal oscillation generator unit, in the debugging stage, for receiving the debugging instruction sent by the electronic model of control unit in system platform unit, corresponding to the electronic model of the element to be detected, and output analog oscillation signal to IO module connector docking unit and the element to be detected;System platform unit, including design drawing.The present application can realize automatic debugging, and debugging and drawing modification are carried out synchronously.

This invention relates to the field of wireless communication technology and discloses a radio frequency front-end ASDIV switching system and electronic device, including a physical antenna group comprising a first antenna and a second antenna; a power amplifier front-end module including a first port and a second port, the first port being directly connected to the first antenna and the second port being directly connected to the second antenna; a transceiver having a main receiver and a diversity receiver; and a switching circuit internally within the power amplifier front-end module. The switching circuit is configured to form four signal paths between the first port, the second port, and the main receiver and diversity receiver, including: a first path from the first port to the main receiver, a second path from the first port to the diversity receiver, a third path from the second port to the main receiver, and a fourth path from the second port to the diversity receiver. By dynamically reconstructing the signal paths through the internal switching circuit to replace an external switch, hardware simplification, performance improvement, and cost reduction are achieved.

The invention belongs to the field of medicinal chemistry, and relates to an indole allylamine amide derivative as well as a preparation method and application thereof. Functional activity screening of G protein dependent signaling pathways is carried out on all the synthesized compounds, and it is found that the new derivatives can be used for preparing beta2-adrenergic receptor allosteric antagonism regulator drugs. Trans-indole allylamine is used as a raw material, amide coupling is performed to obtain the novel indole allylamine amide derivative, and R1 is any one of phenyl, m-methylbenzene, m-methoxyphenyl, m-bromophenyl, m-chlorphenyl, m-fluorophenyl, o-methoxyphenyl, p-methoxyphenyl, naphthalene ring, oxazole, cyclohexyl, cyclopentyl, methyl, ethyl and isopropyl. A biological activity test result shows that the indole allylamine amide derivative disclosed by the invention has relatively good antagonistic activity on beta2AR and can be used for carrying out negative allosteric regulation on the functional activity of isoproterenol.

The invention relates to application of NMRK2 in regulating and improving myocardial ischemia reperfusion injury, and belongs to the technical field of biological medicine. Based on the lack of medicines for effectively preventing and treating myocardial ischemia-reperfusion injury at present, the invention develops a protection method based on NMRK2 signal regulation and control, specifically, a preparation for enhancing NMRK2 expression or activity is adopted to prepare the medicines for preventing or treating myocardial ischemia-reperfusion injury, and the medicines can systematically enhance the anti-oxidation defense capability of myocardial cells, so that the anti-oxidation defense capability of myocardial cells is enhanced. The tissue injury caused by reperfusion is improved, so that the myocardial ischemia reperfusion injury is effectively prevented and treated. Furthermore, it is found that the NMRK2 can inhibit excessive activation of MST1 / p-MST1 in a Hippo pathway and promote YAP nuclear translocation, so that NADK expression is up-regulated, a Trx1 antioxidant system is enhanced, and a new 'NMRK2-YAP-NADK-Trx1 antioxidant signal pathway' is formed.

The present application relates to a kind of drug composition containing STING agonist and WIP1 inhibitor and its liposome and application.The composition can be co-encapsulated in liposome with STING agonist and WIP1 inhibitor, form stable drug delivery system.The liposome can promote efficient endocytosis of cell, and release two active ingredients in cytoplasm synchronously, block the negative feedback mechanism of STING signal path by WIP1 inhibitor, enhance and prolong the activation level of STING path, to synergistically inhibit tumor growth.Based on the mechanism, the drug composition of the present application can be used for preparing the drug for treating diseases related to STING path (such as tumor).

The invention provides a double-pole double-throw radio frequency relay fault real-time detection method and system, and relates to the technical field of radio frequency relay fault detection. The method comprises the following steps: respectively applying a test excitation signal to each independent signal path and acquiring a response signal; performing frequency domain feature extraction on the response signal to obtain a nonlinear distortion feature value representing the physical state of the internal contact of each independent signal path; comparing the nonlinear distortion characteristic value of each independent signal path with the corresponding path independent health reference in real time to obtain a deviation value; and independently judging whether each independent signal path has physical degradation or not according to the deviation value. The method provided by the invention aims to solve the problems of false alarm and missing alarm caused by the difference of the physical characteristics of two independent signal paths when the double-pole double-throw radio frequency relay runs for a long time and the environment is changed in the prior art, and is beneficial to improving the accuracy of fault detection and the efficiency of production testing.

The application provides an automated test bench for multi-device testing and a testing method thereof, and relates to the technical field of device testing. The automated test bench comprises a test bench main body, at least two groups of controller interfaces, at least two groups of driver interfaces and a control panel arranged on the test bench main body. Each controller interface is connected to a programmable logic controller, each driver interface is connected to a driver, the control panel comprises a controller selection switch selected from the at least two groups of controller interfaces as a test interface, a driver selection switch selected from the at least two groups of driver interfaces as a test interface and a switching control module. The switching control module turns on the power loop of the selected controller interface and the driver interface and the signal path between the two, so that the externally connected driver and programmable logic controller can participate in the test. The application solves the problems of fixed matching of the test bench, inability to simulate actual working conditions and mixed use of multi-brand devices.

The embodiment of the invention discloses a heavy chain variable region and a light chain variable region of an anti-GPC3 monoclonal antibody and application of the heavy chain variable region and the light chain variable region. The high-affinity anti-GPC3 sequence is obtained through screening, the binding specificity of the high-affinity anti-GPC3 sequence and the GPC3 antigen is high, and dissociation is slow. The sequence is used as an extracellular targeting domain to construct GPC3-CAR, after macrophages are transduced through lentivirus, CAR-M can specifically recognize GPC3 positive target cells, activate intracellular signal channels and remarkably improve in-vitro phagocytosis and killing activity, the hepatoma cell lysis effect is better, and technical support is provided for anti-hepatoma application.

The invention discloses a double-target chimeric antigen receptor capable of simultaneously targeting TLL1 and B7H3, a CAR-T cell and application of the double-target chimeric antigen receptor. The chimeric antigen receptor is a fusion protein which is sequentially composed of Omburt-scFv (SEQ ID NO: 1) targeting B7H3, a CD8 alpha hinge region and transmembrane region, a 4-1BB costimulatory domain, a CD3 zeta signal domain and TLL1-scFv (SEQ ID NO: 6) targeting TLL1 from an N terminal to a C terminal. The TLL1-scFv can be efficiently combined with TLL1 protein, can inhibit a TGF-beta signal channel and block prostate cancer cell migration, and is integrated into CAR of targeted B7H3, so that the double-target CAR-T cell with direct killing and immune microenvironment regulation functions is successfully constructed. The CAR-T cell has a remarkable killing effect on a prostate cancer cell line DU145, can be strongly activated after being co-cultured with a target cell and secretes a large amount of IFN-gamma and TNF-alpha, and shows high immunocompetence. The invention provides a new synergistic immunotherapy strategy for the B7H3-positive prostate cancer with the TGF-beta signal channel activated.

The application provides a nanobody targeting human transmembrane activator and calcium modulator and cyclophilin interactor (TACI) and an application thereof, and the antibody is derived from a llama heavy chain antibody variable region. The anti-TACI single-domain antibody NB38 can specifically bind to human TACI with high affinity, can recognize a recombinant TACI protein, can also recognize a TACI with a natural conformation on a cell surface, and can partially block a BAFF / APRIL signal pathway. Based on the nanobody, the application constructs a fusion protein, a chimeric antigen receptor, an effector cell expressing the chimeric antigen receptor, and a double-specific cell linker and other genetically engineered forms. The nanobody can be further extended into a drug coupling form. The product of the application can be used for mediating directional recognition and killing of TACI positive cells, and can be used as a supplement and expansion of BCMA targeted therapy, and provides a new candidate technical scheme for targeted therapy of multiple myeloma and other TACI related diseases.

The invention belongs to the technical field of biomedicine, and relates to a novel application of 3-O-methyl chrysotoxin, in particular to an application of 3-O-methyl chrysotoxin in preparation of a medicine for treating xerophthalmia. The core of the 3-O-methyl chrysotoxin disclosed by the invention is that the 3-O-methyl chrysotoxin effectively relieves corneal epithelial cell injury induced by hyperosmosis and inhibits oxidative stress and proinflammatory factor release by activating an EGFR (Epidermal Growth Factor Receptor) signal channel. In-vitro and in-vivo experiments prove that the composition can remarkably enhance cell activity, reduce apoptosis and active oxygen accumulation and repair the steady state of a tear film. The compound is applied to preparation of xerophthalmia drugs and has important clinical value.

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

The invention discloses an application of thermal sensitive Sibestein granules in treating / or preventing asthma, and belongs to the technical field of biological medicines. The invention finds that the thermal sensitive Sibestein granules can reduce the total number of cells and the number of eosinophilic granulocytes, neutrophil granulocytes and lymphocytes in asthma mice BALF, reduce the level of cell factors in asthma mice BALF and IgE in serum, reduce exudation of inflammatory substances in airway and alveolar tissues of asthma mice and infiltration of inflammatory cells, improve the lesion degree of lung tissues, and improve the curative effect of the lung tissues. The action mechanism may be related to regulation and control of MAPK / NF-kappa B signal channel related protein expression, so that inflammatory response and cell apoptosis are inhibited, and airway inflammation of mice is relieved.

The invention discloses a DNA tetrahedron gating logic nanometer machine and a preparation method and application thereof, and belongs to the technical field of biomedicine. According to the invention, a DNA tetrahedron gating logic nanometer machine DTDN is constructed, and the DNA tetrahedron gating logic nanometer machine DTDN is composed of two DNA tetrahedron nanometer structures functionalized by AS1411 aptamers. Each tetrahedron is loaded with a functional DNA hairpin structure, and the structure is composed of a stem-loop structure and an extended single-stranded tail containing an AP site. The framework gating strategy of DTDN enables efficient delivery of functional DNA hairpins, and also prevents signal leakage by blocking cohesive end sequences of HCR reactions. The cascade activated logic loop has high specificity and anti-interference capability, and can accurately activate a downstream regulation and control path. Therefore, the DTDN realizes synchronization of imaging and regulation, and a programmable, safe and accurate nucleic acid nano platform is provided for cancer diagnosis and accurate treatment based on a cGAS-STING signal channel.

The application discloses a kind of small molecule compound combination and the method for preparing vascular endothelial cell using the small molecule compound combination induced differentiation cell.The method is directed to induce differentiated cell, and finally prepare vascular endothelial cell;The directional induction includes inhibiting the activity of lysine deacetylase, inhibiting the signal path of TGF-β, inhibiting the activity of DNA methyltransferase (DNMT), activating the signal path of WNT / β-catenin and activating the signal path of cAMP.The present application can be converted into vascular endothelial cell by small molecule compound combination induction, and each step can realize accurate quality control, which is convenient for standardized operation and large-scale production.The method provided by the present application has wide donor sources, and the patient himself can be used as a donor, so that the vascular endothelial cells required for basic research, clinical treatment or tissue engineering production can be obtained in a short time.

The application discloses an azaphilone molecule targeting STAT3 signal pathways, a preparation method and purposes. The structural formula of the azaphilone molecule chaetoviridinol A1 is shown as formula (A). The azaphilone molecule chaetoviridinol A1 or a medicinal salt thereof has the purposes of preparing an antitumor drug.

This application provides a device and method for processing spatial environmental noise in electronic devices. The device includes: a communication antenna for receiving communication signals and spatial environmental noise; a detection antenna for real-time detection of the spatial environmental noise; a switching network connecting the signal paths of the communication antenna and the detection antenna respectively, for switching the device's operating mode; a signal processing module connected to the signal path of the detection antenna for amplifying, attenuating, and phase-shifting the detected noise signal; a coupler whose through port is connected to the signal path of the communication antenna, its coupling port is connected to the signal path of the detection antenna, and its output is connected to a radio frequency system; and a control module for adjusting the attenuation and phase shift of the signal processing module according to the noise signal parameters received by the communication antenna and the detection antenna, so that the two noise signals are superimposed in antiphase in the coupler. This application improves the receiving performance of wireless communication devices through active detection and cancellation.