25 results about "Mitochondrial respiratory chain" patented technology

The invention discloses a probe combination for detecting a mitochondrial respiratory chain super complex based on a proximity ligation assay and a hybridization chain reaction and application, and belongs to the field of probe detection. Two adjacent protein subunits capable of reflecting the structural integrity of the hypercomplex are determined as bases, the antigen-antibody combination principle is utilized to promote the antigen-antibody which recognizes and combines the bases to initiate proximity ligation reaction (PLA) due to nucleic acid labeling, cascade amplification of detection signals is realized according to the hybridization chain reaction (HCR) principle, and the detection sensitivity of the hypercomplex is improved. The activity of the oxidative phosphorylation system is judged through the structural integrity of the super-complex, the defects and limitation of a traditional detection method are overcome, and rapid, high-sensitivity and accurate detection on the structure of the super-complex is achieved. Meanwhile, the cells are fixed on a 96-hole fluorescent plate, and the structural integrity of the mitochondrial hypercomplex is detected by using a multifunctional microplate reader, so that parallel detection on 96 samples at the same time is finally realized, and digitization and high throughput of detection are realized.

Disclosed in the present invention are a mitochondrial-localized small peptide (MLSP) and the use thereof in improving the plant yield and / or stress resistance. By means of overexpressing the mitochondrial-localized small peptide (MLSP) in plants, the present invention improves the stability and activity of the mitochondrial respiratory chain in plants and improves the activity of plant mitochondrial complexes, thus improving the plant yield and resistance to drought and saline-alkali stress.

The application belongs to the technical field of functional drinks, and particularly relates to a compound functional beverage for improving tissue cell hypoxia and a preparation method and application thereof. 12 The compound functional beverage is prepared by using high-oxygen liquid as a base, and compounding vitamin B1, vitamin B2 and vitamin B6, wherein the high-oxygen liquid is prepared by a low-temperature oxygenation or ozone-charging process, can be absorbed through the digestive tract to realize intestinal oxygen delivery, directly improve the blood oxygen partial pressure and blood oxygen saturation of the body, and eliminate lactic acid accumulation; the water-solubility of the B-group vitamins is good, and the chemical properties are stable, so that the B-group vitamins can provide synthesis materials for coenzyme of the mitochondrial respiratory chain, and improve the oxygen uptake and utilization capacity of cells. The two can break the vicious cycle of hypoxia inducible factor-1 alpha and lactic acid, and improve the hypoxia state of tissue cells. The application can improve the blood lactic acid clearance rate of the sports population by 20% to 25%, and improve the blood oxygen saturation under the highland environment by 5% to 8%, and can also be used for hypoxia auxiliary intervention and chemotherapy sensitivity enhancement of tumor radiotherapy and chemotherapy, and is suitable for all kinds of hypoxia population.

The present invention belongs to the technical field of pesticide preparations, and specifically relates to a pesticide composition for peanut white rot and a preparation method thereof, comprising three active ingredients, Fe2 (EDTA) 3-fluoxazolidinone nanocomposite, prothioconazole and azoxystrobin, wherein the mass ratio of the Fe2 (EDTA) 3-fluoxazolidinone nanocomposite, prothioconazole and azoxystrobin is 6-8:1:1. The pesticide composition for peanut white rot in the present invention is obtained by complexing EDTANa2 with iron ions to obtain Fe2 (EDTA) 3, which can be used as a synergist of fluoxazolidinone to improve the bactericidal effect of fluoxazolidinone, and on the other hand, iron also plays an important role in the electron transfer of the mitochondrial respiratory chain, and can synergistically enhance the bactericidal effect of fluoxazolidinone, so as to achieve a better bactericidal effect.

The invention belongs to the technical field of biochemical energy batteries, and discloses an implantable biochemical energy battery array, a chip and application. Comprising a plurality of biochemical energy power generation units which are mutually connected through a microfluidic channel to form a biochemical energy power generation array, the power generation array is connected in series and in parallel to form a battery array, and glucose fluid flows through the microfluidic channel. The power generation unit comprises a positive electrode, a biochemical decomposition chamber and a negative electrode, carrier materials of hexokinase HK, glucose 6-phosphate dehydrogenase G6PD, glucose 6-phosphate lactonase PGLS and glucose 6-phosphate dehydrogenase 6PGDH are fixed in the biochemical decomposition chamber, and the negative electrode is coated with an artificial film assembled with NADPH transhydrogenase and mitochondrial respiratory chain compound protein. According to the invention, glucose is used as fuel of the cell for continuous power generation, and the flexible array biochemical energy cell which is miniaturized, high in load, long in endurance, capable of being shaped and high in energy density can be provided.

The application discloses a kind of nano preparation for treating ischemia-reperfusion injury and its preparation method and application, belong to the field of pharmaceutical preparation, the nano preparation includes active ingredient, albumin, liposome phospholipid bilayer and target head, active ingredient is composed of antioxidant and coenzyme Q10 and its analog, liposome phospholipid bilayer is composed of phospholipid, cholesterol and DSPE-mPEG 2000 Composition, the nano preparation can both remove active oxygen produced in cell and relieve intracellular oxidative stress, also can enter mitochondrial inner membrane and help mitochondrial respiratory chain electron transfer, repair damaged mitochondrial function, reduce the generation of active oxygen from root, so as to play the effect of treating both symptoms and root cause.The application proposes a kind of new strategy for treating ischemia-reperfusion injury related diseases, by removing active oxygen, effectively restoring blocked electron transport chain, improving the antioxidant capacity of cell itself, reducing oxidative damage caused by ferroptosis, with good practical application prospect.

The invention discloses a mitochondrial small peptide MLSP and an application in improving plant yield and / or stress resistance, the mitochondrial small peptide MLSP is over-expressed in a plant, the stability and activity of a plant mitochondrial respiratory chain are improved, the activity of a plant mitochondrial compound is improved, and then the plant yield is improved. And the resistance to drought and saline-alkali stress is generated.

The present invention discloses a probe combination and application for detecting mitochondrial respiratory chain supercomplexes based on proximity ligation technology and hybridization chain reaction, belonging to the field of probe detection. By identifying two adjacent protein subunits that reflect the structural integrity of the supercomplex as pedestals, and utilizing the principle of antigen-antibody binding, the antigen-antibody that recognizes and binds to the pedestals triggers a proximity ligation reaction (PLA) due to nucleic acid labeling. Based on the principle of hybridization chain reaction (HCR), cascade amplification of the detection signal is achieved, and the activity of the oxidative phosphorylation system is determined by the structural integrity of the supercomplex. This overcomes the shortcomings and limitations of traditional detection methods and enables rapid, highly sensitive, and accurate detection of supercomplex structure. Furthermore, by fixing cells on a 96-well fluorescence plate and using a multifunctional microplate reader to detect the structural integrity of the mitochondrial supercomplex, the system ultimately enables parallel testing of up to 96 samples, achieving digital and high-throughput detection.

To provide a new treatment means for cancer treatment.SOLUTION: The present invention relates to a metabolic inhibitor comprising 7-carbomethoxy-5-hydroxy-2-methyl-1, 4-naphthoquinone (compound KISO079) which inhibits both mitochondrial respiratory chain and glycolytic system in cells, an antitumor agent or a pharmaceutical composition comprising the inhibitor, a method for treating cancer using the antitumor agent, and the like.SELECTED DRAWING: Figure 6

The invention belongs to the technical field of pesticide compounding, and particularly relates to a composition for preventing and treating pine wood nematodes, a preparation and application of the composition and the preparation. Effective active components in the composition comprise an active component A and an active component B, the concentration interaction ratio of the active component A to the active component B is 1: (0.1-10), the active component A is abamectin or emamectin benzoate, and the active component B is rotenone. Plant source active components and chemical pesticide components are compounded to improve the prevention and control effect, the pesticide dosage is reduced, the environmental risk is reduced, a binary compound system of rotenone and abamectin chemical agents is innovatively constructed, and the pesticide composition has a good application prospect in prevention and control of rotenone and abamectin chemical agents. Through cooperation of different action mechanisms of inhibition of mitochondrial respiratory chains of the two and blocking of glutamic acid gated chloride ion channels, multi-target, low-risk and high-efficiency prevention and control targets are achieved, and a green and efficient compound pesticide is provided for green and sustainable prevention and control of pine wood nematode diseases.

The present invention relates generally to isolated mitochondria derived from mtDNA-depleted cells (RhoO-mito) that are advantageously used as delivery vehicles for heterogenous proteins or nucleic acids or chemical agents. In some embodiments, proteins or nucleic acids or chemical agents are introduced into a cell, transported into, transferred or expressed in the mitochondria, thereafter or before mtDNA is removed and mtDNA-free mitochondria are extracted from the cell. These mitochondria are used for therapeutic treatment of mitochondria-related diseases such as maple syrup syndrome, citrin deficiency, Nieman-Pick disease, X-linked adrenoleukodystrophy (X-ALD), Pyruvate Dehydrogenase Deficiency, respiratory chain diseases, mutations in mitochondrial respiratory chain proteins, fatty acid metabolism disorders, or cancer with reduced likelihood of causing an immunogenic response. Additionally, in some embodiments the present invention relates to isolated mitochondria, including but not necessarily isolated mitochondria derived from mtDNA-depleted cells (RhoO-mito), that are exogenously introduced into cells to enhance production of endogenous or heterogenous proteins.

The invention belongs to the technical field of compound enzyme activity inhibitors, and particularly relates to application of benzovindiflupyr in preparation of a mitochondrial respiratory chain compound II inhibitor. The benzovindiflupyr has relatively strong inhibition capability on the activity of a mitochondrial respiratory chain compound II, such as a human mitochondrial respiratory chain compound II, and can effectively inhibit the activity of the human mitochondrial respiratory chain compound II at a low nanomole level.

A novel cellular product composed of chemically-treated macrophages for treating fibrosis diseases. The cellular product has the beneficial effect of protecting organs against fibrosis-induced lesions and dysfunction by reducing collagen content. The macrophages are treated with mitochondrial respiratory chain inhibitors and cultured under hypoxia conditions. They express a higher level of anti-fibrosis bio-markers and a reduced level of pro-fibrosis bio-markers. The macrophages can be administered to animals with pulmonary fibrosis via tail vein or intratracheal injection. The invention can also be used to treat patients with Idiopathic Pulmonary Fibrosis, liver fibrosis, myocardial fibrosis, and renal fibrosis. A novel technique produces and manufactures cellular products for therapeutic purposes by using tissue culture vehicles coated with temperature-responsive polymer material. This novel technique has a clear advantage in growing more cells and preserving cell integrity compared to conventional methods.

The invention relates to application of an OGN overexpression vector in preparation of a medicine for preventing reperfusion myocardial injury, and belongs to the technical field of biological medicine. Aiming at the problem that an existing mitochondrial targeting strategy is insufficient in multi-target cooperation in MI / RI prevention, the invention provides application of an OGN overexpression vector in preparation of drugs for preventing reperfusion myocardial injury, and the OGN overexpression vector is a recombinant adeno-associated virus vector and contains a nucleotide sequence for coding OGN. According to the invention, three-dimensional mitochondrial steady state regulation of myocardial ischemia reperfusion injury is realized for the first time, and myocardial energy metabolism is remarkably improved and cell death is reduced by enhancing mitochondrial respiratory chain activity, stabilizing membrane potential and inhibiting ROS outbreak, so that heart failure caused by MI / RI is effectively reversed. Experimental data confirm that AAV-mediated OGN overexpression has a protective effect on cardiac functions and has remarkable clinical transformation potential.

The invention discloses a nano preparation for treating ischemia reperfusion injury and a preparation method and application thereof, and belongs to the field of pharmaceutical preparations, the nano preparation comprises an active component, albumin, a liposome phospholipid bilayer and a target head, the active component is composed of an antioxidant and coenzyme Q10 and analogues thereof, and the target head is composed of a liposome phospholipid bilayer and a liposome phospholipid bilayer. The lipidosome phospholipid bilayer is composed of phospholipid, cholesterol and DSPE-mPEG2000, the nano preparation can remove active oxygen generated in cells to relieve intracellular oxidative stress, and can enter mitochondrial inner membranes to help mitochondrial respiratory chain electron transfer and repair damaged mitochondrial functions, generation of the active oxygen is reduced from the source, and the nano preparation can be used for treating the mitochondrial respiratory chain electron transfer and repairing the damaged mitochondrial function. Therefore, the effect of treating both symptoms and root causes is achieved. The invention provides a new strategy for treating ischemia-reperfusion injury related diseases, the oxidation resistance of cells is improved and oxidative injury caused by ferroptosis is reduced by removing active oxygen and effectively recovering a blocked electron transport chain, and the application has a relatively good practical application prospect.

The invention relates to the technical field of cosmetics, in particular to application of 3-phenyl lactate or a fermentation product containing the 3-phenyl lactate in preparation of a skin care product and the skin care product. According to the application disclosed by the invention, an in-vitro cell model is adopted for carrying out a function test on 3-phenyl lactate, and in a keratinocyte model, the 3-phenyl lactic acid monoglyceride has a remarkable anti-oxidation effect and a remarkable cell proliferation promoting capability, so that oxidative stress injury can be reduced, and epidermis repair and regeneration can be promoted; in a fibroblast model, the 3-phenyl lactic acid monoglyceride remarkably promotes collagen expression, enhances expression of SKN-1 and ATFS-1 signal channel related genes and mitochondrial respiratory chain complex II and V related genes, and can enhance the cell oxidation resistance, improve the energy metabolism efficiency and promote protein synthesis and repair, so that the fibroblast has the advantages that the fibroblast has a good application prospect. Skin elasticity can be improved, skin aging can be relieved, and skin injury repair can be promoted.

The present application finds that the activity of mitochondrial respiratory chain complex V of patients with idiopathic pulmonary fibrosis is significantly lower than that of healthy people, and finds that the increase of TGF-β is related to the decrease of the activity of mitochondrial respiratory chain complex V of patients with pulmonary fibrosis, thereby proving that the mitochondrial respiratory chain complex V is a molecular marker for the diagnosis of idiopathic pulmonary fibrosis. Further, the present application discloses the use of a reagent for detecting the activity of mitochondrial respiratory chain complex V in the preparation of a composition or kit for assisting in the diagnosis of idiopathic pulmonary fibrosis, and further discloses a composition for assisting in the diagnosis of idiopathic pulmonary fibrosis, which comprises a reagent for detecting the activity of mitochondrial respiratory chain complex V, and a kit for assisting in the diagnosis of idiopathic pulmonary fibrosis, and a method for screening an agent for treating idiopathic pulmonary fibrosis in vitro.

The invention discloses application of N-acetylcysteine in preparation of a medicine for enhancing an immune effect, and relates to the technical field of biology. On the basis of the existing vaccine immunomodulator technology, the invention provides an application of N-acetylcysteine or pharmaceutically acceptable salt thereof as an active ingredient in preparation of drugs for enhancing the vaccine immune effect and / or for adjuvant treatment of infection, and the N-acetylcysteine can be used for enhancing the immune effect of the vaccine by directly protecting the mitochondrial respiratory chain function of plasma cells. According to the present invention, with the application of the traditional immunomodulator, the low-level intracellular active oxygen can be maintained, and the life in the bone marrow can be prolonged so as to provide the unique advantages in immune reaction and memory enhancement, such that the long-term immune protection effect can be improved, and the safety problem such as local or whole body adverse reaction possibly caused by the traditional immunomodulator can be avoided; a new safe and effective source is provided for the existing vaccine immunomodulator and the medicine for treating infection, and the application prospect is wide.

The invention discloses poplar and phellinus igniarius polysaccharide SVP-50-1 as well as a preparation method and application thereof, and belongs to the field of biological medicines. The poplar phellinus igniarius polysaccharide SVP-50-1 is obtained by carrying out water extraction on poplar phellinus igniarius sporocarp and precipitating the poplar phellinus igniarius sporocarp with a 50% ethanol solution. The poplar phellinus igniarius polysaccharide SVP-50-1 provided by the invention can regulate the tumor metabolism process and inhibit the mitochondrial respiratory chain activity and the oxidative phosphorylation process of tumor cells, so that the energy source of the tumor cells is inhibited, and the anti-tumor effect is achieved. When the poplar and phellinus igniarius polysaccharide SVP-50-1 is combined with radiotherapy and chemotherapy drugs for use, a synergistic effect can be achieved. According to the combined treatment strategy, the curative effect of the existing tumor therapy can be remarkably improved, meanwhile, adverse reactions are reduced, and a new optimization scheme is provided for clinical tumor treatment.

Compositions and methods for treatment of mitochondrial respiratory chain dysfunction and other mitochondrial disorders are provided. Also disclosed are a number of screening assays having utility for the identification of agents which modulate the phenotype associated with mitochondrial respiratory chain dysfunction.

The invention belongs to the technical field of pesticides, and relates to an acaricidal composition containing a mitochondrial respiratory chain inhibitor, the acaricidal composition comprises an active component A and an active component B, the active component A is a mitochondrial respiratory chain inhibitor, and the active component B is fluoromite sulfide. The acaricidal composition or the preparation thereof can enhance the pesticide effect, enlarge the acaricidal spectrum, reduce the use cost, reduce the dosage, prolong the lasting period and delay the development of pesticide resistance.

The application relates to application of an OGN overexpression vector in preparation of a reperfusion myocardial injury prevention drug and belongs to the technical field of biological medicines. In view of the problem that existing mitochondrial targeting strategies are insufficient in multi-target point cooperation in MI / RI prevention, the application provides application of an OGN overexpression vector in preparation of a reperfusion myocardial injury prevention drug. The OGN overexpression vector is a recombinant adeno-associated virus vector and comprises a nucleotide sequence coding OGN. The application first realizes three-dimensional mitochondrial steady-state regulation of myocardial ischemia-reperfusion injury, significantly improves myocardial energy metabolism and reduces cell death by enhancing mitochondrial respiratory chain activity, stabilizing membrane potential and inhibiting ROS explosion, thereby effectively reversing heart function failure caused by MI / RI. Experimental data prove that AAV-mediated OGN overexpression has a significant clinical conversion potential.

This invention discloses the application of a peptide SRD in the preparation of drugs for treating acute kidney injury, belonging to the field of biomedical technology. In an in vitro H2O2-induced oxidative stress injury experiment on renal tubular epithelial cells, this invention found that peptide SRD treatment significantly reduced the apoptosis rate of renal tubular cells damaged by oxidative stress, promoted ATP production, and restored mitochondrial respiratory chain function. In an animal model of ischemia-reperfusion-induced acute kidney injury, mice with acute kidney injury injected via the tail vein showed significantly decreased serum creatinine and blood urea nitrogen levels. Pathological observation revealed a significant reduction in the degree of renal tubular injury, a lower histological score, and a significant downregulation of KIM-1, an indicator of the degree of renal tubular injury. This indicates that the antioxidant peptide SRD can improve mitochondrial function in renal tubular cells, effectively reduce renal tubular cell damage, promote the repair of renal tubular epithelial cells in acute kidney injury (AKI), effectively improve renal function, and has a good therapeutic effect on acute kidney injury.

The invention provides a molecular marker for identifying the aging process of watermelon seeds, the molecular marker comprises Cla97C04G070560 and Cla97C03G062270, and the sequences of the molecular marker and the Cla97C03G062270 are as shown in SEQ ID NO: 1 and SEQ ID NO: 2; the Cla97C04G070560 is used for coding cytochrome c oxidase, is an important protein in a mitochondrial respiratory chain, participates in an oxidative phosphorylation process, and has high conservative property in biotaxonomy; the Cla97C03G062270 is used for coding ribosomal protein, plays an important role in a protein synthesis process, and participates in assembly and stabilization of ribosomes. The invention further provides an identification method and application of the molecular marker. The application comprises the step of applying the molecular marker to watermelon seed aging detection. Through the molecular marker for the aging process of the watermelon seeds, the aging degree of the seeds can be evaluated by detecting the expression level of the aging gene marker, so that people can be helped to quickly detect and screen out the batches of the aged seeds, and the molecular marker has important significance for ensuring high-quality seed production and brings a more convenient means for subsequent breeding.

The invention belongs to the technical field of pesticide preparations, and particularly relates to a pesticide composition for peanut southern blight and a preparation method thereof, the pesticide composition comprises three active components of a Fe2 (EDTA) 3-fluxapyroxad nanocomposite, prothioconazole and azoxystrobin, and the mass ratio of the Fe2 (EDTA) 3-fluxapyroxad nanocomposite to the prothioconazole to the azoxystrobin is (6-8): 1: 1. According to the pesticide composition for peanut southern blight, EDTANa2 and iron ions are complexed to obtain Fe2 (EDTA) 3, on one hand, the Fe2 (EDTA) 3 can be used as a synergist of fluxapyroxad to improve the bactericidal effect of fluxapyroxad, and on the other hand, the iron element also plays an important role in electron transfer of a mitochondrial respiratory chain, so that the synergistic effect of the Fe2 (EDTA) 3 and the mitochondrial respiratory chain is improved. The sterilization effect of the fluxapyroxad can be synergistically enhanced, and the sterilization effect is better.