22 results about "Mitochondrial electron transport" patented technology

The application belongs to the field of optical functional composite material application, and discloses a preparation method of conjugated oligomer / gold nanocluster light energy conversion material. The preparation method is to synthesize conjugated oligomer material with main chain of fluorene and thiophene and side chain containing active groups, and to compound the conjugated oligomer material with gold nanocluster containing mercapto ligand through covalent connection to prepare conjugated oligomer / gold nanocluster compound. The preparation method is simple and easy to operate, and can effectively compound two kinds of semiconductor materials. The nanocomposite has a size of about 2 nanometers and has the ability to quickly enter photosynthetic organisms and structures. The conjugated oligomer / gold nanocluster heterojunction is cultured with photosynthetic organisms and structures, and under sunlight irradiation, high-efficiency photo-generated electrons are generated and quickly transferred to the electron transport chain of the photosynthetic organisms and structures, so as to improve the solar energy conversion efficiency, increase the biomass yield, and have practical application value in the field of energy conversion.

The invention discloses a gram-positive bacterium extracellular electron transport chain construction method and a synthetic microbial agent. It is found that after a lipophilic material is added into an iron reduction system, the iron reduction speed and the iron reduction amount of gram-positive bacteria with weak electron transfer capacity are remarkably increased, and the iron reduction amount of the gram-positive bacteria can be consistent with the iron reduction magnitude of gram-negative strong iron reduction bacteria. The lipophilic degree and the iron reduction rate are subjected to correlation analysis and show significant positive correlation, which reveals that the lipophilic degree and the permeable membrane capacity of the material are key parameters for determining the iron reduction capacity of gram-positive bacteria. The lipophilic material not only can smoothly penetrate through the cell wall of gram-positive bacteria, but also can be enriched on a cell membrane in quantity, and gradually permeates into a cytoplasm matrix to generate electron exchange with NADH (Nicotinamide Adenine Dinucleotide Hormone) so as to construct a gram-positive bacteria extracellular electron transport chain. The invention provides a brand-new strategy for preparing the biological geological catalytic synthetic microbial inoculum for soil iron reduction.

This invention relates to the field of biological denitrification technology, and more particularly to a microbial electron-regulated denitrification system based on nitrogen-doped carbon dots and its application. The system comprises electron-donating microorganisms, electron-accepting microorganisms, and nitrogen-doped carbon dots. The nitrogen-doped carbon dots act as extracellular electron mediators, constructing an efficient extracellular electron transport network and reducing interspecies electron transport resistance. Simultaneously, the nitrogen-doped carbon dots can regulate intracellular electron transport chains and energy metabolism processes, promoting increased reducing power and energy levels, and optimizing metabolic patterns. By synergistically regulating electron generation, transport, and utilization under oligotrophic conditions with limited electron donors, this system achieves highly efficient denitrification, avoids nitrite accumulation, and significantly inhibits nitrous oxide (N2O) generation, while reducing dependence on external organic carbon sources and electron sources. This provides a new technical approach for improving the efficiency of deep denitrification under low-carbon conditions.

The application provides a compound extract for treating gastric ulcer, a preparation method and application thereof, and belongs to the technical field of medicines. The compound extract is obtained by using medicinal materials of pachouli and high kankyo through an ethanol degreasing, water extraction and alcohol precipitation process. The application has the advantages of few medicinal materials, simple extraction and preparation process and easy industrialization. Animal efficacy and mechanism researches show that the compound extract can protect the gastric mucosal barrier by synergistically improving the function of the mitochondrial electron transport chain and enhancing the antioxidant stress capacity, and can effectively prevent and treat gastric ulcer.

This invention discloses a three-component oxidation system and its application in the degradation of 4-hydroxyestradiol. Specifically, it discloses a 4,5-exogeneic dioxygenase, HedG1, associated with the oxidative cleavage of 4-OH-E1. This enzyme, supported by an electron transport chain composed of ferrugin HedG2 and ferrugin reductase HedG3, catalyzes the 4,5-seco meta-oxidative cleavage of 4-OH-E1, generating a meta-cleavage product of the A ring. The three-component dioxygenase system HedG1G2G3 based on HedG1 in this invention rapidly reduces the hormonal activity of cyclopentane polyhydrophenanthrene by disrupting the A ring of the cyclopentane polyhydrophenanthrene structure, which is beneficial for the remediation and treatment of environmental estrogen pollution.

The present application provides a kind of fluorine boron compound, the fluorine boron compound contains anthraquinone group, can generate strong oxidizing anion radical after photoexcitation efficiently, thus has lower half inhibitory concentration, stronger inhibitory effect, has stronger phototoxicity, can kill cancer cells in shorter time;Meanwhile, the superoxide anion radical generated can specifically oxidize FADH2 coenzyme in mitochondrial electron transport chain, by oxidizing its isoalloxazine ring structure to cause coenzyme inactivation, then mitochondrial electron transport process, and inhibit cancer cell energy metabolism, so as to effectively inhibit the proliferation activity of cancer cells.

The invention discloses low-temperature injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Hydrogel is prepared from high-molecular-weight hyaluronic acid grafted with vitamin E and poly (N-isopropylacrylamide), the hydrogel is in an injectable liquid state at 4 DEG C, and a drug capsaicin (CPZ) wrapped by micelle is loaded and injected into the brain from the brain in a stereotactic mode. The pharmaceutical composition can temporarily maintain local low temperature of brain tissue and release capsaicin, reduce local metabolic activity of ischemic brain tissue, temporarily inhibit biogenesis, reduce NADH, FADH2 and other reducing equivalents and ADP accumulation, reduce the possibility of electron leakage in an electron transport chain, and improve the bioavailability of ischemic brain tissue. Vitamin E is added to reduce superoxide anions (O2 <.->) generated by reduction reaction of oxygen and leaked electrons in the reperfusion process, and vitamin E can promote ROS removal, synergistically alleviate oxidative stress and subsequent inflammation, and comprehensively improve cerebral ischemia reperfusion injury.

The present application relates to a kind of composition containing mitochondrial electron transport inhibitor class insecticide and its application.The compound I has synergistic effect with mitochondrial electron transport inhibitor class insecticide, can improve pest control effect, reduce application amount, reduce use frequency, reduce use cost.

The application discloses a kind of nano preparation for treating ischemia-reperfusion injury and its preparation method and application, belong to the field of pharmaceutical preparation, the nano preparation includes active ingredient, albumin, liposome phospholipid bilayer and target head, active ingredient is composed of antioxidant and coenzyme Q10 and its analog, liposome phospholipid bilayer is composed of phospholipid, cholesterol and DSPE-mPEG 2000 Composition, the nano preparation can both remove active oxygen produced in cell and relieve intracellular oxidative stress, also can enter mitochondrial inner membrane and help mitochondrial respiratory chain electron transfer, repair damaged mitochondrial function, reduce the generation of active oxygen from root, so as to play the effect of treating both symptoms and root cause.The application proposes a kind of new strategy for treating ischemia-reperfusion injury related diseases, by removing active oxygen, effectively restoring blocked electron transport chain, improving the antioxidant capacity of cell itself, reducing oxidative damage caused by ferroptosis, with good practical application prospect.

The application discloses olympicene or a pharmaceutically acceptable salt thereof, which has a structure shown in formula (I). The olympicene or the pharmaceutically acceptable salt thereof shown in formula (I) has low cytotoxicity, can activate the synthesis of glutathione after entering cells, and can strengthen the antioxidant capacity of cells. Meanwhile, the metabolism of the olympicene or the pharmaceutically acceptable salt thereof can up-regulate the expression of genes in an electron transport chain in mitochondria, so that the effect of promoting mitochondrial metabolism is achieved.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

A composition including a compound of formula (I)and one or more mitochondrial electron transport system complex III inhibitor(s) selected from the group consisting of azoxystrobin, coumoxystrobin, enoxastrobin, flufenoxystrobin, picoxystrobin, pyraoxystrobin, pyraclostrobin, mandestrobin, pyrametostrobin, triclopyricarb, kresoxim-methyl, trifloxystrobin, dimoxystrobin, fenaminstrobin, metominostrobin, orysastrobin, famoxadone, fluoxastrobin, fenamidone, pyribencarb, metyltetraprole, cyazofamid, amisulbrom, fenpicoxamid, and florylpicoxamid.

The present application relates to the technical field of nanocomposite, and particularly relates to a photocatalytic nanocomposite, a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, and has the advantages of simple process, low energy consumption and high production efficiency, and can realize efficient and low-cost large-scale preparation of the photocatalytic nanocomposite. The photocatalytic nanocomposite is composed of two kinds of semiconductor photocatalytic materials with staggered energy bands, can not only adjust the light absorption range, but also realize effective separation of photo-generated electron-hole pairs by transferring photo-generated electrons and holes to different semiconductor photocatalytic materials. The photo-generated electrons and holes generated by light excitation can make the composite material have strong oxidation-reduction capacity, and can be used for preparing products for adjusting mitochondrial function, so as to accurately interfere with the mitochondrial electron transport chain and realize oxidation of NADH and reduction of cytochrome C.

The invention relates to the technical field of antibacterial coatings, in particular to a titanium-based implant antibacterial coating, a preparation method of the titanium-based implant antibacterial coating and a prepared titanium-based implant. The coating is a Bi-TCPP compound formed by coordination bonding of bismuth ions and tetra (4-carboxyphenyl) porphyrin, and is covalently fixed on the surface of the titanium-based implant through a silane coupling agent layer. The preparation method comprises the following steps: performing cleaning and alkali heat treatment on a titanium substrate to construct an active surface; grafting a coupling agent layer through silanization reaction; the preparation method comprises the following steps: preparing a Bi-TCPP compound by a solvothermal method; and immobilizing the compound on the surface of a substrate through amidation reaction. According to the coating, efficient sterilization is achieved through pseudo-iron metabolism interference of bismuth ions and the double synergistic effect of TCPP on a bacterial membrane electron transfer chain, selective antibiosis is achieved through the structural difference between bacteria and human cells, and cytotoxicity is effectively avoided. The coating has the ultrasonic response characteristic, on-demand controlled release of antibacterial factors can be achieved, and the antibacterial efficiency and durability are remarkably improved.

Paenibacillus bacterial strains are engineered to express or overexpress (i) one or more proteins (pyruvate ferredoxin oxidoreductase, flavodoxin, and / or ferredoxin) involved in the electron transport chain, (ii) the DUF protein and / or (iii) gene products of the suf operon. This enables a greater rate / level of nitrogen fixation by the diazotrophic bacteria. The engineered bacteria are introduced to agricultural plants, or seeds for growing such, forming a symbiotic relationship that results in increased nitrogen availability to the plant and improved plant agronomic traits.

The application provides a high positive charge self-assembled ruthenium complex targeting electron transport chain and a preparation method and application thereof, and belongs to the technical field of biological medicines.The ruthenium complex provided by the application has a structural formula I shown in formula I; wherein 1<=n<=15.The high positive charge self-assembled ruthenium complex targeting electron transport chain is synthesized for the first time, the complex shows high-efficiency anti-MRSA activity, and can eliminate bacteria without toxic side effects, thereby showing good high-efficiency low-toxicity antibacterial activity, and providing a novel high-efficiency high-biocompatibility antibacterial strategy for solving problems such as antibiotic abuse and antibiotic resistance of bacteria.

Paenibacillus bacterial strains are engineered to express or overexpress (i) one or more proteins (pyruvate ferredoxin oxidoreductase, flavodoxin, and / or ferredoxin) involved in the electron transport chain, (ii) the DUF protein and / or (iii) gene products of the suf operon. This enables a greater rate / level of nitrogen fixation by the diazotrophic bacteria. The engineered bacteria are introduced to agricultural plants, or seeds for growing such, forming a symbiotic relationship that results in increased nitrogen availability to the plant and improved plant agronomic traits.

The application discloses an electron transfer chain subunit acCYTB with low-temperature activity and application thereof, and relates to the fields of genetic engineering and enzyme engineering technology. The electron transfer chain subunit provided by the application is any one of the following (1), (2) and (3): (1) an acCYTB protein, wherein the amino acid sequence is shown as SEQ ID NO. 2; (2) a protein obtained by substituting, deleting and / or adding one or more amino acid residues in the amino acid sequence of the acCYTB protein, wherein the protein has more than 90% identity with the acCYTB protein and has the same biological function as the acCYTB protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transfer chain subunit can improve the low-temperature resistance of organisms, and thus can be applied to low-temperature trait improvement and industrial production.

The application discloses an electron transfer chain subunit with low-temperature activity, a coding gene and application thereof, and relates to the technical fields of genetic engineering and enzyme engineering. The electron transfer chain subunit is any one of the following (1), (2) and (3): (1) a hoNDUFA12 protein, the amino acid sequence of which is shown in SEQ ID NO. 2; (2) a protein with more than 90% identity with the hoNDUFA12 protein and the same biological function as the hoNDUFA12 protein, which is obtained by substituting, deleting and / or adding one or more amino acid residues to the amino acid sequence of the hoNDUFA12 protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transfer chain subunit can improve the low-temperature resistance of organisms, and thus can be applied to low-temperature trait improvement and industrial production.

The invention relates to the technical field of nano composite materials, in particular to a photocatalytic nano composite material as well as a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, is simple in process, low in energy consumption and high in production efficiency, and can realize high-efficiency and low-cost large-scale preparation of the photocatalytic nano composite material. The photocatalytic nano composite material is composed of two semiconductor photocatalytic materials with staggered energy bands, so that not only can the absorption range of light be adjusted, but also the effective separation of photo-induced electron-hole pairs can be realized by transferring photo-induced electrons and holes to different semiconductor photocatalytic materials. Photogenerated electrons and holes generated by light excitation enable the composite material to obtain relatively strong oxidation-reduction ability, and the composite material can be used for preparing products for adjusting mitochondrial functions so as to accurately interfere mitochondrial electron transport chains and realize oxidation of NADH and reduction of cytochrome C.

The present application relates to a composition containing a biologically effective amount of compound I and a mitochondrial electron transport inhibitor class insecticide, and their methods for controlling pests in agronomic and non-agronomic environments. The compound I has a synergistic effect with the mitochondrial electron transport inhibitor class insecticide, which can improve the pest control effect, reduce the application amount, reduce the use frequency, and reduce the use cost.

A pharmaceutical composition for an ophthalmic use either in humans or mammalians for a treatment of eye disorders is provided. The pharmaceutical composition includes an effective amount of an oxidizing agent and an effective amount of a photo-sensitizing agent, in combination with pharmaceutically acceptable excipients; the photo-sensitizing agent is a molecule having an absorbance peak in a wavelength range between 340 nm and 440 nm and emits fluorescence in a wavelength range between 450 nm and 600 nm; the oxidizing agent is chosen among molecules, wherein the molecules partially or totally inhibit a mitochondrial electron transport chain activity, wherein the pharmaceutical composition is a liquid formulation further includes one or more substances chosen among: diluents, permeabilizers, disinfectants, buffer systems, salts, antiseptics, and the pharmaceutically acceptable excipients.