49 results about "Mitochondrial electron transport" patented technology

The invention discloses a P450 mutant and application thereof in synthesis of calcifediol, and belongs to the technical field of bioengineering. According to the present invention, the P450 enzyme mutant enzyme is constructed, and the single plasmid three-enzyme co-expression system of the redox partner and the cofactor cycle enzyme is constructed, such that the sufficient electron transport chain is provided for the P450 enzyme, and the calcifediol can be efficiently synthesized under the mild condition by using the vitamin D3 as the substrate; the conversion rate of calcifediol can reach 63.1%, and the conversion rate of the reaction is greatly improved. By adopting the method disclosed by the invention, the catalytic efficiency of the catalyst is improved, and the reaction cost is reduced.

The invention provides a pharmaceutical composition containing a pyrazole ether compound and application of the pharmaceutical composition, the pharmaceutical composition comprises an effective component A and an effective component B. The effective component A is a pyrazole ether compound with a structure shown in a formula I; and the effective component B is selected from any one or a combination of at least two of azole bactericides, mitochondrial electron transport chain compound III inhibitor bactericides, SDHI bactericides, multi-site contact type bactericides or other bactericides. In the invention, the effective component A and the effective component B have a synergistic effect, so that the pharmaceutical composition has higher biological activity and favorable or wider active spectrum, can reduce the application amount of compounds and compositions required for effectively controlling phytopathogens, and has longer lasting activity.

The invention discloses an electron transport chain subunit acCYTB with low-temperature activity and application of the electron transport chain subunit acCYTB, and relates to the technical field of gene engineering and the technical field of enzyme engineering. The electron transport chain subunit provided by the invention is any one of the following (1), (2) and (3): (1) acCYTB protein, the amino acid sequence of which is as shown in SEQ ID NO.2; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the acCYTB protein to obtain a protein which has more than 90% of identity with the acCYTB protein and has the same biological function with the acCYTB protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transport chain subunit can improve the low-temperature resistance of organisms, so that the electron transport chain subunit is applied to low-temperature character improvement and industrial production.

Efficient lung cancer treatment is challenging due to the lack of therapeutic targets. The succinate dehydrogenase (SDH) enzyme plays a critical metabolic role as an intermediate between the citric acid cycle and the electron transport chain associated with the cancer disease state. Although several existing compounds have been applied to target metabolic diseases in vitro, modulating SDH for lung cancer treatment has been elusive. By integrating the AtomNet® technology for compound identification with mitochondria- and cell-based enzyme activity assays, this disclosure demonstrates identification of four new small molecules that bind directly to SDH subunits in lung cancer cells. Mechanistically, treatment with these small molecules increased reactive oxygen species and DNA damage in lung cancer cell lines. Functionally, these small molecules reduced short-term and long-term malignant growth of lung cancer cell lines in vitro. This study provides a new therapeutic approach for targeting lung and other types of cancers through modulation of SDH.

The invention discloses a preparation method and application of polyethylene glycol functionalized nano capsule-like gel. The preparation method comprises the following steps: (1) extracting a nano capsule-like body; (2) preparing polyethylene glycol functionalized nano capsule-like gel; and (3) applying the polyethylene glycol functionalized nano capsule-like gel to mediating photodynamic tooth bleaching. The polyethylene glycol functionalized nano capsule-like gel is combined with a photodynamic reaction to interfere with an electron transport chain and promote ROS accumulation, and the nano capsule-like gel with a specific photoreaction oxidation function is constructed, so that the nano capsule-like gel can be used for equivalently bleaching teeth with a traditional bleaching agent, and meanwhile, the activity of the nano capsule-like gel is improved. The sensitive risk to tooth essence and the mechanical injury risk to enamel and cementum are remarkably reduced, and the composition can be applied to home care or clinical whitening treatment; and the preparation method is simple, is convenient to use, is mild and non-irritant, is high in biocompatibility, and has a good market application prospect.

The invention discloses application of a mitochondrial differentiation expression gene in preparation of a depression diagnostic agent and a depression treatment drug, and relates to the technical field of depression diagnosis and treatment. And the mitochondrial differentiation expression gene comprises one or more of TFAM (Transforming Factor Amplification Molecular), SURF1 and NDUFB9. According to the present invention, the SURF1 gene simultaneously expressed in inhibitory neurons and excitatory neurons is focused on, the key effect of the SURF1 in CUMS-induced depression-like behaviors is found, the CUMS-induced molecular and behavior changes are effectively reversed by the overexpression of the SURF1, and the potential anti-depression effect is displayed; besides, animal experiments find that overexpression of the SURF1 can effectively recover the function of an electron transport chain, ATP generation is enhanced, mitochondrial membrane potential is maintained, and oxidative stress is reduced. The effects jointly relieve depression-like behaviors induced by CUMS, and further support the protection effect of the SURF1 in a depression model, which provides evidence for the new effect of the SURF1 in the pathophysiology of depression.

This application provides a method for extracting gold resources from urban minerals based on TEMPO electrocatalysis-histidine gold leaching reagent system, which belongs to the technical field of precious metal recycling. This method utilizes the original copper / iron impurities in discarded printed circuit boards to construct a Cu 2+ / Fe 3+ TEMPO redox coupled synergistic catalytic system. 2+ (E°=0.34 V vs SHE) and Fe 3+ (E°=0.77 V) with TEMPO + (E°=0.75 V) forms a step-by-step electron transport chain. TEMPO is precisely controlled by electric field + The electron transfer efficiency of the TEMPO redox couple (E° = 0.75 V vs. SHE) significantly increases the gold dissolution rate while reducing energy consumption and achieving a low impurity co-dissolution rate, enabling efficient and selective recovery of gold resources from electronic waste. This invention, through the TEMPO electrocatalytic-histidine chelation synergistic system, achieves efficient and selective extraction of gold from urban minerals, fundamentally addressing the key challenges of traditional cyanide-free gold extraction processes, such as high oxidant consumption, poor selectivity, and low efficiency.

The application belongs to the field of optical functional composite material application, and discloses a preparation method of conjugated oligomer / gold nanocluster light energy conversion material. The preparation method is to synthesize conjugated oligomer material with main chain of fluorene and thiophene and side chain containing active groups, and to compound the conjugated oligomer material with gold nanocluster containing mercapto ligand through covalent connection to prepare conjugated oligomer / gold nanocluster compound. The preparation method is simple and easy to operate, and can effectively compound two kinds of semiconductor materials. The nanocomposite has a size of about 2 nanometers and has the ability to quickly enter photosynthetic organisms and structures. The conjugated oligomer / gold nanocluster heterojunction is cultured with photosynthetic organisms and structures, and under sunlight irradiation, high-efficiency photo-generated electrons are generated and quickly transferred to the electron transport chain of the photosynthetic organisms and structures, so as to improve the solar energy conversion efficiency, increase the biomass yield, and have practical application value in the field of energy conversion.

The invention discloses a gram-positive bacterium extracellular electron transport chain construction method and a synthetic microbial agent. It is found that after a lipophilic material is added into an iron reduction system, the iron reduction speed and the iron reduction amount of gram-positive bacteria with weak electron transfer capacity are remarkably increased, and the iron reduction amount of the gram-positive bacteria can be consistent with the iron reduction magnitude of gram-negative strong iron reduction bacteria. The lipophilic degree and the iron reduction rate are subjected to correlation analysis and show significant positive correlation, which reveals that the lipophilic degree and the permeable membrane capacity of the material are key parameters for determining the iron reduction capacity of gram-positive bacteria. The lipophilic material not only can smoothly penetrate through the cell wall of gram-positive bacteria, but also can be enriched on a cell membrane in quantity, and gradually permeates into a cytoplasm matrix to generate electron exchange with NADH (Nicotinamide Adenine Dinucleotide Hormone) so as to construct a gram-positive bacteria extracellular electron transport chain. The invention provides a brand-new strategy for preparing the biological geological catalytic synthetic microbial inoculum for soil iron reduction.

The invention discloses engineering bacteria, a biosensor and a detection method thereof. According to the sensor, modified Acinetobacter bayli ADP1 is taken as a carrier, and exogenous nucleic acid can be autonomously absorbed without external induction by utilizing the natural nucleic acid uptake capability of the carrier; after nucleic acid internalization, hairpin structure limitation of the blocking type gRNA is relieved, a CRISPRa system is activated, dCas9-SoxS protein is driven to be combined with a target promoter in a targeted mode, CymA-MtrCAB gene expression is started, and specific recognition of a nucleic acid sequence is achieved. The system is integrated with a CymA-MtrCAB transmembrane electron conduction module, a biological recognition signal is converted into an electrochemical signal in real time to be output through a membrane protein mediated electron transfer chain, and a nucleic acid recognition-gene activation-electric response detection path is established. The biosensor has the advantages of structural modularization, high response specificity, high detection sensitivity, wide environment adaptability and the like, is suitable for tumor marker detection, wearable health monitoring and other biomolecule recognition scenes, and provides a novel biosensing solution which is high in universality and programmable.

The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novel Thermococcus strain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.

The invention belongs to the technical field of electrocatalysis, and discloses a chlorella electrode with redox activity and a preparation method and application thereof. Under a constant potential window, methylene blue, aniline and chlorella are synchronously subjected to electrochemical oxidation to generate free radicals, an electron transport chain between chlorella and methylene blue is constructed through a click reaction, meanwhile, an interpenetrating network structure copolymer is generated in the copolymerization process, and chlorella is wrapped and anchored on the surface of an electrode. According to the method, chlorella can be effectively dispersed, more active sites are exposed, the electron transfer efficiency is improved, and the stability of the electrode is enhanced under the interaction of the three. The obtained chlorella electrode with redox activity has an application prospect in the field of electrocatalytic reduction of carbon dioxide into high-added-value products.

This invention relates to the field of biological denitrification technology, and more particularly to a microbial electron-regulated denitrification system based on nitrogen-doped carbon dots and its application. The system comprises electron-donating microorganisms, electron-accepting microorganisms, and nitrogen-doped carbon dots. The nitrogen-doped carbon dots act as extracellular electron mediators, constructing an efficient extracellular electron transport network and reducing interspecies electron transport resistance. Simultaneously, the nitrogen-doped carbon dots can regulate intracellular electron transport chains and energy metabolism processes, promoting increased reducing power and energy levels, and optimizing metabolic patterns. By synergistically regulating electron generation, transport, and utilization under oligotrophic conditions with limited electron donors, this system achieves highly efficient denitrification, avoids nitrite accumulation, and significantly inhibits nitrous oxide (N2O) generation, while reducing dependence on external organic carbon sources and electron sources. This provides a new technical approach for improving the efficiency of deep denitrification under low-carbon conditions.

The application provides a compound extract for treating gastric ulcer, a preparation method and application thereof, and belongs to the technical field of medicines. The compound extract is obtained by using medicinal materials of pachouli and high kankyo through an ethanol degreasing, water extraction and alcohol precipitation process. The application has the advantages of few medicinal materials, simple extraction and preparation process and easy industrialization. Animal efficacy and mechanism researches show that the compound extract can protect the gastric mucosal barrier by synergistically improving the function of the mitochondrial electron transport chain and enhancing the antioxidant stress capacity, and can effectively prevent and treat gastric ulcer.

This invention discloses a three-component oxidation system and its application in the degradation of 4-hydroxyestradiol. Specifically, it discloses a 4,5-exogeneic dioxygenase, HedG1, associated with the oxidative cleavage of 4-OH-E1. This enzyme, supported by an electron transport chain composed of ferrugin HedG2 and ferrugin reductase HedG3, catalyzes the 4,5-seco meta-oxidative cleavage of 4-OH-E1, generating a meta-cleavage product of the A ring. The three-component dioxygenase system HedG1G2G3 based on HedG1 in this invention rapidly reduces the hormonal activity of cyclopentane polyhydrophenanthrene by disrupting the A ring of the cyclopentane polyhydrophenanthrene structure, which is beneficial for the remediation and treatment of environmental estrogen pollution.

The invention relates to application of an agonist for improving the expression quantity of an SLC25A51 gene or protein in preparation of a medicine for preventing or treating intrahepatic cholestasis in a gestation period. The invention further relates to a method for constructing a novel animal model for the intrahepatic cholestasis in the gestation period by feeding 0.5% cholic acid. Research is carried out from multiple levels of animal, cell and molecular levels, and by discussing that SLC25A51 transports NAD + into mitochondria, expression of mitochondrial electron transport chain related genes is promoted, the content of malic acid in tricarboxylic acid cycle is increased, proliferation of placental vascular endothelial cells with intrahepatic cholestasis in gestation period is promoted, and the placental vascular endothelial cells with intrahepatic cholestasis in gestation period are promoted. It is proved that activation of the SLC25A51 plays an important role in improving angiogenesis, and the SLC25A51 can be used as a new target for screening intrahepatic cholestasis in the gestation period.

The present application provides a kind of fluorine boron compound, the fluorine boron compound contains anthraquinone group, can generate strong oxidizing anion radical after photoexcitation efficiently, thus has lower half inhibitory concentration, stronger inhibitory effect, has stronger phototoxicity, can kill cancer cells in shorter time;Meanwhile, the superoxide anion radical generated can specifically oxidize FADH2 coenzyme in mitochondrial electron transport chain, by oxidizing its isoalloxazine ring structure to cause coenzyme inactivation, then mitochondrial electron transport process, and inhibit cancer cell energy metabolism, so as to effectively inhibit the proliferation activity of cancer cells.

A method of diagnosing the likelihood of recurrence of clear cell renal cell carcinoma is provided. The method involves a) detecting the gene expression signatures of mitochondrial electron transport chain subunits, mitochondrial ribosomal proteins, major histocompatibility complex class II (MHC-II) proteins or combinations thereof in a kidney tumor tissue sample; and b) determining that the subject has an elevated risk of recurrence of clear cell renal cell carcinoma if the gene expression signatures include certain sequences. In another embodiment, the method uses copper levels to diagnose the likelihood of recurrence of clear cell renal cell carcinoma.

The invention discloses low-temperature injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Hydrogel is prepared from high-molecular-weight hyaluronic acid grafted with vitamin E and poly (N-isopropylacrylamide), the hydrogel is in an injectable liquid state at 4 DEG C, and a drug capsaicin (CPZ) wrapped by micelle is loaded and injected into the brain from the brain in a stereotactic mode. The pharmaceutical composition can temporarily maintain local low temperature of brain tissue and release capsaicin, reduce local metabolic activity of ischemic brain tissue, temporarily inhibit biogenesis, reduce NADH, FADH2 and other reducing equivalents and ADP accumulation, reduce the possibility of electron leakage in an electron transport chain, and improve the bioavailability of ischemic brain tissue. Vitamin E is added to reduce superoxide anions (O2 <.->) generated by reduction reaction of oxygen and leaked electrons in the reperfusion process, and vitamin E can promote ROS removal, synergistically alleviate oxidative stress and subsequent inflammation, and comprehensively improve cerebral ischemia reperfusion injury.

The present invention provides a biomimetic liposome nanoreactor, a preparation method and its application, and relates to the field of biomedical engineering technology. The biomimetic liposome nanoreactor comprises liposomes, thylakoid membrane fragments and electron donors. The present invention integrates thylakoid fragments (Tk) into liposomes (Lip) and loads electron donors into the liposomes to prepare a biomimetic liposome nanoreactor. The biomimetic liposome nanoreactor of the present invention can be taken up by renal epithelial cells and catalyze NAD + Generate; and under the drive of ultrasound, the electron transport chain on it transfers a large number of electrons to the cytoplasm, converting NAD + Reduction to NADH promotes ATP production, rapidly alleviating AKI in the early stages. Furthermore, in the presence of electrons, the effective generation of NADPH fundamentally prevents the generation of oxidative stress such as ROS, effectively preventing the progression of AKI to CKD.

The present application relates to a kind of composition containing mitochondrial electron transport inhibitor class insecticide and its application.The compound I has synergistic effect with mitochondrial electron transport inhibitor class insecticide, can improve pest control effect, reduce application amount, reduce use frequency, reduce use cost.

The application discloses a kind of nano preparation for treating ischemia-reperfusion injury and its preparation method and application, belong to the field of pharmaceutical preparation, the nano preparation includes active ingredient, albumin, liposome phospholipid bilayer and target head, active ingredient is composed of antioxidant and coenzyme Q10 and its analog, liposome phospholipid bilayer is composed of phospholipid, cholesterol and DSPE-mPEG 2000 Composition, the nano preparation can both remove active oxygen produced in cell and relieve intracellular oxidative stress, also can enter mitochondrial inner membrane and help mitochondrial respiratory chain electron transfer, repair damaged mitochondrial function, reduce the generation of active oxygen from root, so as to play the effect of treating both symptoms and root cause.The application proposes a kind of new strategy for treating ischemia-reperfusion injury related diseases, by removing active oxygen, effectively restoring blocked electron transport chain, improving the antioxidant capacity of cell itself, reducing oxidative damage caused by ferroptosis, with good practical application prospect.

The invention relates to the technical field of agriculture, in particular to a compound herbicide for siberian cocklebur and a preparation method thereof, and the compound herbicide comprises 150 ml of benazolin, 60 ml of florasulam, 1.5 g of alkyl polyglycoside, 7 g of polyvinyl alcohol, 100 g of diatomite and 70 ml of distilled water. According to the compound herbicide, synergistic interaction of multiple action mechanisms is realized through scientific proportioning. The benazolin is used as a benzothiadiazinone herbicide and can inhibit an electron transport chain in plant photosynthesis; the florasulam is used as an acetolactate synthetase inhibitor and can block synthesis of branched chain amino acid. After the two components are compounded, the risk of drug resistance caused by long-term use of a single component is remarkably reduced. A composite herbicide is stirred through stirring blades, the composite herbicide is extruded through movement of the stirring blades, the extruded composite herbicide is crushed through crushing blades, and finally crushed composite herbicide particles are dried, so that the preparation process of the composite herbicide is simplified; the preparation efficiency of the compound herbicide is improved.

The application discloses olympicene or a pharmaceutically acceptable salt thereof, which has a structure shown in formula (I). The olympicene or the pharmaceutically acceptable salt thereof shown in formula (I) has low cytotoxicity, can activate the synthesis of glutathione after entering cells, and can strengthen the antioxidant capacity of cells. Meanwhile, the metabolism of the olympicene or the pharmaceutically acceptable salt thereof can up-regulate the expression of genes in an electron transport chain in mitochondria, so that the effect of promoting mitochondrial metabolism is achieved.

Nutraceutical compositions and the methods for promoting wakefulness and alertness by the administration thereof. The nutraceutical compositions address the challenged state of the individual to remain awake and alert during waking hours. The methods provide the nutraceutical composition directly to the cells in the brain and body where energy is produced and utilized. A nutraceutical composition includes a therapeutically effective amount of Vitamin B1, Vitamin B2, Vitamin B3, Vitamin B5, Vitamin B6, Vitamin B12, citicoline sodium, calcium pyruvate, and L-tyrosine, wherein said therapeutically effective amount promotes wakefulness by providing molecular support for glycolysis, Krebs cycle reactions, and electron transport chain (ETC) activity. The composition can further include Vitamin C and caffeine.

The invention discloses an electron transport chain subunit with low-temperature activity as well as a coding gene and application thereof, and relates to the technical fields of gene engineering and enzyme engineering. The electron transport chain subunit is any one of the following (1), (2) and (3): (1) hoNDUFA12 protein, the amino acid sequence of which is shown as SEQ ID NO.2; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the hoNDUFA12 protein to obtain a protein which has more than 90% of identity with the hoNDUFA12 protein and has the same biological function with the hoNDUFA12 protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transport chain subunit can improve the low-temperature resistance of organisms, so that the electron transport chain subunit is applied to low-temperature character improvement and industrial production.

The invention belongs to the technical field of algae development experiments, and particularly relates to a method for measuring energy metabolism of chlorella mitochondrial stress cells. According to the method, an Agilent cell energy meter XF24 is utilized, the setting of an Agilent default experimental program is optimized, and a mixing part in all reading programs is canceled; the drug concentrations of the three targeting drugs added into the mitochondrial electron transport chain are designed through a large number of research tests, and the number of measurement cycles (5-6 times) after the drugs are added is increased, so that the oxygen consumption rate of cells is measured to obtain key parameters reflecting the mitochondrial function, and the measurement result is more accurate. According to the method, a chlorella mitochondrial pressure curve graph is given, and a new research direction is provided for energy metabolism of algae cells.

A composition including a compound of formula (I)and one or more mitochondrial electron transport system complex III inhibitor(s) selected from the group consisting of azoxystrobin, coumoxystrobin, enoxastrobin, flufenoxystrobin, picoxystrobin, pyraoxystrobin, pyraclostrobin, mandestrobin, pyrametostrobin, triclopyricarb, kresoxim-methyl, trifloxystrobin, dimoxystrobin, fenaminstrobin, metominostrobin, orysastrobin, famoxadone, fluoxastrobin, fenamidone, pyribencarb, metyltetraprole, cyazofamid, amisulbrom, fenpicoxamid, and florylpicoxamid.

The present application relates to the technical field of nanocomposite, and particularly relates to a photocatalytic nanocomposite, a preparation method and application thereof. The preparation method comprises three steps of molybdenum disulfide nanoparticle preparation, precursor dispersion and in-situ compounding, and has the advantages of simple process, low energy consumption and high production efficiency, and can realize efficient and low-cost large-scale preparation of the photocatalytic nanocomposite. The photocatalytic nanocomposite is composed of two kinds of semiconductor photocatalytic materials with staggered energy bands, can not only adjust the light absorption range, but also realize effective separation of photo-generated electron-hole pairs by transferring photo-generated electrons and holes to different semiconductor photocatalytic materials. The photo-generated electrons and holes generated by light excitation can make the composite material have strong oxidation-reduction capacity, and can be used for preparing products for adjusting mitochondrial function, so as to accurately interfere with the mitochondrial electron transport chain and realize oxidation of NADH and reduction of cytochrome C.

The invention relates to the technical field of antibacterial coatings, in particular to a titanium-based implant antibacterial coating, a preparation method of the titanium-based implant antibacterial coating and a prepared titanium-based implant. The coating is a Bi-TCPP compound formed by coordination bonding of bismuth ions and tetra (4-carboxyphenyl) porphyrin, and is covalently fixed on the surface of the titanium-based implant through a silane coupling agent layer. The preparation method comprises the following steps: performing cleaning and alkali heat treatment on a titanium substrate to construct an active surface; grafting a coupling agent layer through silanization reaction; the preparation method comprises the following steps: preparing a Bi-TCPP compound by a solvothermal method; and immobilizing the compound on the surface of a substrate through amidation reaction. According to the coating, efficient sterilization is achieved through pseudo-iron metabolism interference of bismuth ions and the double synergistic effect of TCPP on a bacterial membrane electron transfer chain, selective antibiosis is achieved through the structural difference between bacteria and human cells, and cytotoxicity is effectively avoided. The coating has the ultrasonic response characteristic, on-demand controlled release of antibacterial factors can be achieved, and the antibacterial efficiency and durability are remarkably improved.