21 results about "Chitin synthesis" patented technology

The invention provides a chitin synthetase 2 gene of diplodia juncoides and application of the chitin synthetase 2 gene, dsRNA for preventing and treating the diplodia juncoides and application of the dsRNA, and relates to the technical field of biology. According to the invention, through a bioinformatics technology, the Hypochloa diplosa CHS2 gene is determined, the growth of the Hypochloa diplosa can be effectively inhibited by silencing the gene, and the prevention and control of the Hypochloa diplosa can be realized. The dsRNA designed and obtained based on the gene can mediate silencing of the CHS2 gene of the diplodia juncosa, and specifically realizes prevention and control of the diplodia juncosa; poisoning of chemical pesticides to non-target beneficial insects is avoided; the use of chemical pesticides is reduced, and the pollution to the environment is reduced.

The present invention provides a chitin synthesis inhibitor compound-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is buprofezin, lufenuron, cyromazine, diafenthiuron, diflubenzuron, chlorbenzuron, hexaflumuron or chlorfluazuron, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially brown planthopper, beet armyworm or liriomyza sativae. The invention verifies that the binary composition of the inhibitor insecticide synthesized from the flufenutraniliprole and the chitin has a remarkable synergistic effect, effectively improves the control effect, reduces the dosage and expands the insecticidal spectrum. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I).

The invention discloses a chitin synthase 1 gene and application thereof in prevention and treatment of diplodia juncoides, and belongs to the technical field of genetic engineering and biological prevention and treatment. The nucleotide sequence of the chitin synthetase 1 gene is as shown in SEQ ID NO. 1. The invention also provides dsRNA (double-stranded ribonucleic acid) for preventing and treating diplodia diplosa, and the dsRNA is formed by transcription of the nucleotide sequence as shown in SEQ ID NO.3. The invention discloses the chitin synthetase 1 gene of the diplodia juncoides for the first time, and designs dsRNA capable of preventing and treating the diplodia juncoides and transgenic corn for expressing the dsRNA of the diplodia juncoides based on the gene. By feeding the transgenic corn leaves, the death rate of the leucotina diplosa can be remarkably increased, and the leaf damage area and degree can be reduced. The invention provides a new gene resource and an efficient and environment-friendly prevention and control method for prevention and control of the diplodia juncoides, provides a new choice for prevention and control of the diplodia juncoides, and has a wide application prospect.

The invention belongs to the field of insecticides and acaricides, and relates to a composition containing alanine-isoxazoline compounds and acaricides. The composition contains an active component A and an active component B, wherein the weight part ratio of the active component A to the active component B is (1: 100)-(100: 1); the active component A is selected from a compound I, a compound II or a stereoisomer thereof; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delaying and the like, and can be used for preventing and treating various pests.

This invention discloses a chitin synthase 1 gene and its application in controlling the two-spotted leaf beetle, belonging to the fields of genetic engineering and biological control technology. The nucleotide sequence of the chitin synthase 1 gene is shown in SEQ ID NO.1. This invention also provides a dsRNA for controlling the two-spotted leaf beetle, transcribed from the nucleotide sequence shown in SEQ ID NO.3. This invention discloses for the first time the chitin synthase 1 gene of the two-spotted leaf beetle, and designs a dsRNA based on this gene capable of controlling the two-spotted leaf beetle, as well as transgenic maize expressing the two-spotted leaf beetle dsRNA. Feeding transgenic maize leaves significantly increases the mortality rate of the two-spotted leaf beetle and reduces the area and severity of leaf damage. This invention provides a new genetic resource and an efficient and environmentally friendly control method for the two-spotted leaf beetle, offering a new option for its control and possessing broad application prospects.

The invention belongs to the technical field of animal models, and relates to a double-stranded RNA and a method for constructing a peritrophic membrane damaged macrobrachium rosenbergii animal model by using the same. The double-stranded RNA enables the chitin synthetic protein gene to be silenced after transcription, the coding sequence of the chitin synthetic protein gene is shown as SEQ ID NO.2, and the amino acid sequence of the chitin synthetic protein coded by the double-stranded RNA is shown as SEQ ID NO.3. According to the present invention, after the double-stranded RNA prepared by using the preparation method is introduced into the macrobrachium rosenbergii body, the chitin synthetic protein gene of the macrobrachium rosenbergii can be transcribed and silenced, such that the peritrophic membrane injury macrobrachium rosenbergii animal model can be constructed, and the peritrophic membrane injury macrobrachium rosenbergii animal model can be used for disease control research, microecological preparation development and / or molecular assisted breeding.

This invention discloses the chitin synthase 1 (LmCHS1) gene of the leafminer moth, its dsRNA, its synthesis method, and its insect-resistant applications. Through transcriptome analysis, specific primer pairs for the open reading frame of the LmCHS1 gene were designed and synthesized. The LmCHS1 gene sequence was obtained through gene cloning (nucleotide sequence: SEQ ID NO: 1, amino acid sequence: SEQ ID NO: 2). The gene fragment with sequence SEQ ID NO: 3 was selected as the RNAi target sequence. Primer pairs SEQ ID NO: 6 and SEQ ID NO: 7 with restriction enzyme sites were designed and synthesized. PCR amplification was performed, and a prokaryotic expression vector was constructed to induce dsRNA expression. Feeding leafminer moth larvae with dsRNA interfered with LmCHS1 gene expression. After 24 hours, abnormalities were observed in the larvae's body surface, with some larvae failing to complete molting or dying due to an inability to form a normal body surface after molting. After 96 hours, the larval mortality rate was 72.22%, compared to 40% in the GFP control group, demonstrating a significant effect. This invention has broad application prospects in the control of the leaf borer pest in forestry due to its high efficiency, specificity, and safety.

The invention relates to a solid molded body for external use against parasites on animals. The molded body of the invention includes, as active compounds, the combination of fipronil and bifenthrin. In addition, the insect growth regulators pyriproxyfen and methoprene may be added as additional actives.

The application belongs to the technical field of biological enzymes, and particularly relates to an expression and purification method of a chitin synthase from a virus and application thereof. The expression and purification method of the chitin synthase from the virus comprises the following steps: a chitin synthase gene sequence from a virus is codon-optimized according to the codon preference of Escherichia coli, so that a nucleotide sequence as shown in the gene sequence of SEQ ID NO. 1 is obtained; the optimized gene sequence is synthesized into an expression vector; a host containing the expression vector is cultured, induced, lysed and centrifuged, so that a membrane component containing the chitin synthase from the virus is obtained. The application provides a purification method of the chitin synthase from the virus, utilizes the strategy of heterologous expression of Escherichia coli and separation and purification of a biomembrane-like system, and through the strategy, the chitin synthase from the virus with high purity can be obtained. A new method for synthesizing oligochitooligosaccharide is provided, and the reaction system is simple in composition, and the product is easy to separate and purify.

The invention relates to the technical field of aquaculture, and discloses an industrial aquaculture workshop shell shrimp larva metamorphosis development cultivation method, which comprises the following steps: S1, when zoeae enters a metamorphosis development critical window, carrying out hypotonic period stress pretreatment on an aquaculture water body; s2, changing the water body, and adding a water body environment synchronous inducer composed of an ecdysone synthesis key precursor, a GABAergic nerve inhibitor and a chitin synthesis unit at one time; s3, then forcibly stopping feeding the larvae, and then feeding fortified baits; s4, after the larvae are intensively metamorphosed and developed into opossum shrimp larvae, changing a large amount of water to reset the culture environment. Through combination of environmental factor pretreatment, physiological function pulse type induction and a specific nutrition recovery strategy, accurate regulation and control of the larva metamorphosis process are achieved, metamorphosis development synchronism is remarkably improved, the cultivation period is shortened, and the survival rate is remarkably increased.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

The invention provides a phenyl pyrazole derivative, preparation thereof and application of the phenyl pyrazole derivative as an insect growth regulator of a targeted ecdysone receptor. The structural formula is shown as a formula I. The phenylpyrazole related compound provided by the invention has insecticidal activity and molting hormone receptor binding activity. The skeleton has the advantages of simple structure, low cost and the like, and the ecdysone receptor is an insect growth regulator and has the advantages of no toxicity or low toxicity to mammals, low resistance incidence rate, high selectivity and the like. Therefore, as a potential insecticidal active compound, the phenyl pyrazole compound has excellent prospects, and the research and application of environment-friendly pesticides can be promoted by developing and exploring the application of the phenyl pyrazole compound in agricultural activity.

The invention belongs to an enzyme inhibitor, and particularly relates to a chitin synthetase inhibitor (novel acetylglucosamine thiourea glycoside conjugate) as well as preparation and application thereof. The invention relates to an application of a novel acetylglucosamine thiourea glycoside conjugate as shown in a formula I in serving as a chitin synthetase inhibitor. The glycoside conjugate disclosed by the invention is novel in structure, has good biological safety and chitin synthetase inhibitory activity, and has the potential of becoming a novel chitin synthetase inhibitor.

The invention discloses a piperine compound as well as a preparation method and application thereof. The structure of the piperine compound is shown as a formula (I). The compound disclosed by the invention is relatively low in cost and easy to synthesize, and can be used as an inhibitor of chitin synthetase to be applied to prevention and control of crop pathogenic fungi, especially prevention and control of crop fungal diseases represented by rice sheath blight disease, apple rot, wheat stem rot and the like.

The present invention provides a novel insecticidal composition of active compounds comprising Methoxyfenozide and Spinosyns and at least one further active compound selected from the class of chitin synthesis inhibitors, and to methods of controlling pests comprising applying said composition.

The invention discloses application of terphenyl compounds as active ingredients in preparation of pesticide bactericides, and belongs to the technical field of pesticides. In order to develop a novel high-activity antifungal agent based on a chitin synthetase target, a terphenyl compound is extracted from fermentation liquor of streptomyces GS2, and the chemical structural formula of the terphenyl compound is shown in the specification. By detecting the inhibitory activity of the compound on chitin synthetase, it is found that the compound achieves sterilization based on the inhibitory effect on the chitin synthetase, and the inhibitory effect of the compound on the chitin synthetase is equivalent to the inhibitory effect of polyoxin B on the chitin synthetase. In addition, bacteriostatic experiments find that the compound can effectively inhibit sclerotinia sclerotiorum and has a wide application prospect in future pesticide development.

This invention belongs to the field of agricultural technology and discloses a dsRNA biopesticide formulation based on a nanocarrier, its preparation method, and its application. Specifically, it discloses a nanocomposite comprising a nanocarrier and dsRNA, wherein the dsRNA is loaded on the nanocarrier and targets the chitin synthase gene (CHS) or the V-ATPase subunit B gene (V-ATPaseB). This invention provides a nanocomposite containing dsRNA targeting the CHS gene or the V-ATPaseB gene. This nanocomposite, as a novel biopesticide formulation, exhibits good pest control and plant leaf protection effects. The nanocarrier used in this nanocomposite can effectively deliver dsRNA, preventing its degradation within pests and improving delivery efficiency.

This invention provides a chitin synthase 2 gene of the two-spotted leaf beetle and its application, as well as a dsRNA for the control of the two-spotted leaf beetle and its application, relating to the field of biotechnology. This invention uses bioinformatics technology to identify the two-spotted leaf beetle. CHS2 This invention utilizes a gene that, by silencing, can effectively inhibit the growth of the two-spotted firefly beetle, thus achieving control of the beetle. Based on this gene, a dsRNA was designed to mediate the growth of the two-spotted firefly beetle. CHS2 Gene silencing enables specific control of the two-spotted leaf beetle; avoids the toxicity of chemical pesticides to non-target beneficial insects; reduces the use of chemical pesticides and lowers environmental pollution.

The invention discloses chitin synthetase AoChsB and application of the chitin synthetase AoChsB in increasing the yield of equisetin produced through aspergillus oryzae fermentation. The invention provides an application of a substance for inhibiting or reducing or down-regulating expression of a coding nucleic acid of AoChsB protein, or a substance for inhibiting or reducing or down-regulating activity or content of the AoChsB protein in any one of the following substances. (A1) the yield of the equissetin prepared by the aspergillus oryzae is increased; a2) preparing aspergillus oryzae with high yield of the equissetin; the Aspergillus oryzae is Aspergillus oryzae capable of producing equisetin; the chitin synthetase gene AoChsB in aspergillus oryzae is analyzed and identified through genome data and bioinformatics. After the chitin synthetase gene AoChsB in the aspergillus oryzae is knocked out, the yield of the equissetin is greatly improved.

Insect control nanobodies are provided. Accordingly there is provided a nanobody which specifically binds to an insect polypeptide selected from the group consisting of: a polypeptide comprising a chitin binding domain (CBD), V-ATPase subunit c, trehalase, cytochrome p450 monooxygenase, chitin deacetylase, chitin synthase and NPC1 sterol transporter, wherein binding of the nanobody to the insect polypeptide confers an insect control activity to the nanobody. Also provided are polynucleotides encoding the nanobody, host cells expressing the nanobody and methods of using it.

We provide insect control nanobodies. [Solution] A nanobody is provided that specifically binds to an insect polypeptide selected from the group consisting of a chitin-binding domain (CBD), V-ATPase subunit c, trehalase, cytochrome p450 monooxygenase, chitin deacetylase, chitin synthase, and NPC1 sterol transporter, wherein the binding of the nanobody to the insect polypeptide confers insect control activity to the nanobody. A polynucleotide encoding the nanobody, a host cell expressing the nanobody, and its applications are also provided.