55 results about "Toxicant" patented technology

1. Name of the product in this design: Rapid Detection Kit for Toxic Substances. 2. Purpose of this design: This design is used for the rapid detection of toxic substances. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This invention discloses a method for detecting the toxicity threshold of a substance based on a rat drinking experiment. The method of this invention includes the following steps: First, a drinking preference experiment is conducted, and then the toxicity threshold is determined: the amount of substance water and pure water consumed in the drinking preference is compared to determine the time when the rat begins to significantly avoid substance water, the mass of the toxic substance in the rat's body at that time is calculated, and the toxicity threshold of the rat is calculated; (3) dose conversion from rat to human: based on the interspecies dose conversion of body surface area, the toxicity threshold of the rat is first converted into the equivalent dose for humans to obtain the acute exposure dose, and then the acute exposure dose is converted into the chronic exposure dose for humans to obtain the substance toxicity threshold. This invention can accurately determine the toxicity threshold: it achieves toxicity assessment of low-dose exposure through spontaneous recognition behavior; it is easy to operate; and it is applicable to the toxicity assessment and non-destructive threshold determination of various substances such as environmental pollutants, chemical substances, and drugs.

The application provides a toxicant diffusion simulation method based on Fluent, which comprises the following steps: data input, receiving explosion data; first simulation, explosion dispersion simulation according to the explosion data; second simulation, cloud diffusion simulation according to the explosion data and the first simulation result, to obtain a diffusion model. In the application, the concentration and diffusion of toxic substances in a unit space within a short time after the explosion are measured and estimated according to the diffusion model, the explosion process can be simulated and modeled according to the initial explosion data, the radial velocity of explosion diffusion, the concentration of toxic substances, and the size, range, temperature field and pressure field effect of the formed aerosol cloud are obtained, the effective assistance of rescue work under the explosion situation is realized, and the exclusion work or optimization of the space layout of the flammable and explosive scene is realized to improve the safety factor of the scene.

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

The Cardio-Tox Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The invention relates to the field of biological control, in particular to an environment response type African giant snail capsule attractive toxicant and a preparation method and application of the environment response type African giant snail capsule attractive toxicant. The capsule attractant comprises a capsule shell and a composition located in the capsule shell, and the composition is prepared from, by mass, 5%-10% of metaldehyde, 50%-65% of attractant, 30%-40% of macromolecule water-absorbent resin, 0.1%-1% of mildew preventive, 0.1%-1% of preservative and 0.1%-1% of coloring agent. The capsule attractant provided by the invention is ingeniously arranged according to the specific life habits of the African snails, and through various exquisite arrangements of a capsule shell, an internal mildew-proof system, SAP water absorption expansion and water locking, the validity period of the attractant can be remarkably delayed, and the pesticide effect can be highly synchronized with the emergence peak of the African snails; the damage to non-target organisms is greatly reduced through optimal setting of the capsule, and the ecological friendliness is outstanding.

The present application belongs to the field of biological toxicity monitoring and ecological toxicity detection, and particularly relates to a pollutant exposure device suitable for aquatic organisms and a large-scale simulation continuous dilution method. The device comprises a cylinder with an open top, a buffer zone, a toxicant exposure zone and a water outlet zone arranged in sequence in the cylinder, the buffer zone and the toxicant exposure zone, and the toxicant exposure zone and the water outlet zone are separated by a partition, the partition is perforated, the buffer zone, the toxicant exposure zone and the water outlet zone are provided with an internal circulation system, and the internal circulation system comprises a submersible pump, a circulating water pipe and an aeration assembly. The method simulates the condition that a real water body receives sewage by using the device, the dispersion and dilution of pollutants are achieved by water flow and aeration, the circulation of pollutants and water bodies is achieved by a circulating system, the water inlet flow of the device is achieved by water containing pollutants, the real concentration that organisms can contact is simulated, and the method has strong application prospects in the fields of non-destructive online monitoring of sewage and wastewater and toxicity simulation of pollutants entering large-scale water bodies.

The disclosures herein relate to cucurbituril compounds and methods and compositions for reducing the amount of a toxin in a patient by administering to the patient an effective amount of the cucurbituril compound. Also provided are methods and compositions for preventing or treating an overdose from a toxin that has been ingested by a patient.

The invention discloses a JNK recombinant protein as well as a preparation method and application thereof, and relates to the technical field of recombinant protein preparation and purification. The preparation method comprises the following steps: S1, taking cDNA (complementary deoxyribonucleic acid) of cristata plicata as a template, and carrying out PCR (polymerase chain reaction) amplification by adopting a specific primer to obtain an amplification product; s2, connecting the amplification product to a carrier by using a homologous recombination technology to construct a recombinant plasmid; and S3, carrying out induced expression on the recombinant plasmid, and purifying to obtain the JNK recombinant protein. The JNK recombinant protein is prepared, the efficient preparation system conditions of the JNK protein are optimized, the target protein is captured through a nickel column affinity chromatography method, the protection effect of the JNK protein in cell resistance toxicants and the resistance of the JNK protein to H2O2 are evaluated, key data support is provided for subsequent research on development of the efficacy of the JNK protein, and the JNK protein has good application prospects. Recycling of shellfish processing waste is promoted, and experimental reference is provided for deep research and development of shellfish active substances.

The invention relates to a method for simultaneously detecting multiple amatoxin, and relates to the technical field of analysis and detection, and the method comprises the following steps: S1, preparing a sample solution to be detected; s2, detecting the amatoxin in the sample solution to be detected by adopting a liquid chromatography-mass spectrometry method; wherein the chromatographic separation conditions of the liquid chromatography-mass spectrometry method are as follows: a chromatographic column: Kinetex is 2.6 [mu] m XB-C18 100A, and LC Column is 50 * 3 mm; mobile phases: the phase A is formic acid water, the phase B is methanol, and gradient elution is carried out. According to the application, synchronous detection of four target objects (alpha-amanita, beta-amanita, gamma-amanita and dihydroxy sticktight) can be completed within a short time, the sensitivity is very high, the requirement of poison residue analysis can be met, and a powerful technical support is provided for emergency detection of public health emergencies.

The invention provides a pomacea canaliculata egg poisoning reagent and a pomacea canaliculata egg poisoning method, and belongs to the technical field of agricultural pest control. The invention provides a pomacea canaliculata ovum poisoning reagent. The active ingredient of the pomacea canaliculata ovum poisoning reagent comprises an aqueous extract of an oleander raw material; the oleander raw material comprises one or more of leaves, stems and flowers of oleander; the aqueous extract of the oleander raw material contains rutin and oleandrin. The active ingredients of the poisoning agent comprise water-soluble natural active ingredients in the oleander, so that the poisoning agent has the advantage of being environmentally friendly, and the problem that the environment is polluted by a traditional chemical control method is effectively solved. Moreover, the aqueous extract of the oleander raw material contains rutin and oleandrin, and the rutin and the oleandrin have a synergistic effect, so that the poison killing effect on the ampullaria gigas eggs can be remarkably improved, and the problem of low physical prevention and treatment efficiency is solved. The poison reagent provided by the invention is suitable for green and accurate prevention and control of pomacea canaliculata in an agricultural ecosystem, especially a rice field.

Systems and methods for mapping toxicants are disclosed. A toxicant database is generated by preparing data retrieved from multiple data sources. Based on a query, which may include a location, the toxicant database is accessed and a map is generated from the toxicant database using at least the location in the query. A report may be generated that provides remediation and mitigation information. The map and the report are presented in a user interface.

This invention belongs to the field of biomedicine, and specifically relates to the application of FeTMPyP in the preparation of drugs to alleviate ovarian toxicity. This application addresses the issue that combined exposure to MC-LR and NaNO₂ significantly induces nitrification stress in mouse ovaries, leading to follicular barrier disruption and ovarian reproductive endocrine dysfunction. FeTMPyP was selected as an intervention agent, which effectively alleviated nitrification stress induced by combined exposure to MC-LR and NaNO₂ in mouse ovaries, and improved sex hormone disorders, estrous cycle irregularities, and follicular atresia caused by the combined exposure to these two toxins. Simultaneously, it restored the fertility of mice, indicating that FeTMPyP intervention can alleviate ovarian toxicity induced by combined exposure to MC-LR and NaNO₂ in mice. This invention can be used to prepare drugs to prevent and treat diseases related to ovarian toxicity caused by environmental toxin exposure in mice, providing a new approach for the early identification and targeted intervention of ovarian-related diseases.

The invention belongs to the field of rice breeding, and particularly discloses a primer group for detecting a PARMS molecular marker of a rice plant height gene OsPH4 and application of the primer group, dwarf type OsPH4 gene detection is carried out on a rice material through a molecular sequence of the rice plant height gene OsPH4 by applying a PARMS technology, and the rice plant height gene OsPH4 is obtained. The rice dwarf type OsPH4 gene can be rapidly and accurately detected in different germplasm resources such as indica rice and japonica rice. According to the method, tedious procedures such as enzyme digestion, electrophoresis and sequencing are not needed in the detection process, pollution of PCR product aerosol and use of toxic substances such as EB are reduced, meanwhile, foreground selection and background selection can be conducted in the early stage of molecular marker assisted breeding, the background recovery rate is increased, the scale of breeding populations is reduced, the breeding process is accelerated, and the breeding efficiency is improved. The efficient and environment-friendly application of the OsPH4 gene in commercial molecular breeding of rice is facilitated.

The invention aims to provide a method for preparing regulatory T cells and knocking out Stub1 to enhance functions of the regulatory T cells, and belongs to the field of cell preparation. Comprising the following steps: (1) Tregs sorting; (2) carrying out subsequent in vitro amplification on the Tregs; and (3) knocking out Stub1 in the Tregs by using CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Compared with an unedited polyclone Tregs group and an environment poison exposure group, in an environment poison induced immune homeostasis imbalance model, the Stub1 knocked-out Tregs are fed back, so that the immune homeostasis imbalance state of rats is remarkably improved, and tissue damage of immune organs can be effectively repaired. Further detection shows that the Foxp3 protein level in the Tregs in the group of rats is remarkably increased, and meanwhile, the number of Tregs cells is also maintained at a relatively high level. The results prove that the strategy provided by the invention has a remarkable intervention effect on improving immune organ injury in the aspects of enhancing the Tregs inhibition function and maintaining immune balance.

The invention discloses a construction method and application of a sperm-specific hOdf2-GFP transgenic mouse model, and relates to the technical field of reproductive biology and transgenic animal model construction. According to the construction method disclosed by the invention, based on a piggyBac transposon expression vector system, a DNA (Deoxyribose Nucleic Acid) sequence containing a coding human source Odf2 is effectively connected with a Protamine1 promoter. The transgenic positive mouse constructed by the method can specifically express human Odf2, has a dynamic change from sperm to zygote to eight cell stages, realizes early continuous visual tracking of embryos, has genetic stability, keeps expression and phenotype consistency across generations, and has a good application prospect. The method can be applied to the fields of environmental poison evaluation, asthenospermia mechanisms, drug screening and the like.

The Cardio-Tox Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The utility model provides a storage device convenient to check forensic poison acceptance samples, which relates to the technical field of sample storage devices and comprises a machine body, a bottom plate is arranged in the machine body, a vertical column is fixed at the top end of the bottom plate, and a plurality of first telescopic rods are mounted on the outer wall of the vertical column at equal intervals. A storage block is fixed to the output end of each first telescopic rod, and three storage holes are formed in the top end of each storage block. Through the arrangement of a plurality of storage blocks, each sample is stored in the storage blocks, when the sample needs to be taken out, the whole bottom plate is driven to rotate through the operation of a motor, the corresponding storage blocks are moved to a discharging pipe, then the single storage block is driven to move through the operation of a first telescopic rod, and the storage blocks are moved out of the discharging pipe; by arranging the discharging pipe, the problem that a large amount of cold air leaks due to the fact that a top cover in a traditional device is opened can be avoided, and the practicability is higher.

The disclosures herein relate to cucurbituril compounds and methods and compositions for reducing the amount of a toxin in a patient by administering to the patient an effective amount of the cucurbituril compound. Also provided are methods and compositions for preventing or treating an overdose from a toxin that has been ingested by a patient.

The invention provides a preparation method of hydroxycotinin-D3, and aims to solve the problem that existing hydroxycotinin-D3 lacks a reference substance and a deuterated substance and hinders forensic poison identification, 3-(pyrrolidine-2-yl) pyridine is used as a raw material, a product is prepared through deuterium methylation, oxidation, hydroxylation and refining, a final finished product is colorless needle-shaped crystals, and after the colorless needle-shaped crystals are dried at 45 DEG C for 72 hours, the hydroxycotinin-D3 is obtained. According to the present invention, the yield is 56% based on the crude product, the HPLC detection results show that the purity is more than 99.7%, the single impurity content is less than 0.1%, the total impurity content is less than 0.3%, the whole product has high stability and high reaction specificity from the intermediate to the finished product, the final finished product completely meets the strict requirements of the reference substance for forensic poison identification, the prepared hydroxycotinin-D3 has advantages of high reaction efficiency, good product quality and the like.

The invention relates to the technical field of sewage treatment, and discloses weeding pesticide wastewater multi-stage treatment equipment, when high-pressure wastewater is output through a water inlet pipe, inclined flow deflectors are pushed to move, a plurality of groups of flow deflectors are matched with one another and are pushed to rotate by the wastewater, so that rotational flow is generated in a treatment tank shell, and rotational turbulent flow impacts a biological membrane treatment assembly I; under the action of high-speed rotational flow, strong shearing force is generated, and a boundary layer on the surface of the biological membrane is continuously updated, so that the mass transfer efficiency of oxygen and organic substrates into the biological membrane is enhanced, metabolites and toxic substances are promoted to diffuse outwards, an anaerobic environment and toxic substance accumulation are effectively prevented from being formed in the biological membrane, and the biological membrane is effectively protected. The thickness of the biological membrane is controlled in an optimal active state, and finally, the pollutant degradation efficiency and the system stability are greatly improved.

The invention belongs to the field of biological medicine, and relates to a lung poison cell screening model and construction and application thereof. The poison cell model provided by the invention contains a PPAR gamma promoter gene segment, a PPRE response element promoter gene segment and a reporter gene segment. The cell model can more sensitively and rapidly detect unknown toxicants with lung toxicity and also can perform early evaluation on whether drugs have side effects such as lung toxicity and the like, and has a good application prospect.

The present disclosure provides a 3-phenoxybenzoic acid-glucuronic acid conjugate, and a preparation method and use thereof, and belongs to the technical field of pesticide detection. Compared with 3-phenoxybenzoic acid, the 3-phenoxybenzoic acid-glucuronic acid conjugate provided by the present disclosure features structural stability, high specificity, long limit of detection, and high content in urine, and can better serve as a marker that identifies whether an organism is killed due to pyrethroid pesticide poisoning. Namely, the 3-phenoxybenzoic acid-glucuronic acid conjugate can detect whether a toxicant (pyrethroid pesticides) is taken antemortem or exposed postmortem, and has an excellent application prospect in pyrethroid pesticide detection. The present disclosure provides a preparation method of a 3-phenoxybenzoic acid-glucuronic acid conjugate. The preparation method provided by the present disclosure features high product yield, simple operation, wide raw material sources, low costs, and suitability for industrial production.

The invention relates to a water treatment system suitable for utilization in mobile platform technologies so as to remove TNT and potentially other energetic materials, toxicants and particulate matter from water and a process of utilizing said system.

The invention discloses a diversified regeneration method of an inactivated SCR (Selective Catalytic Reduction) denitration catalyst, which comprises the following steps: firstly, carrying out apparent inspection, performance test and comprehensive analysis on the inactivated catalyst to determine the variety, distribution and degree of toxic substances; next, physical, chemical, thermal regeneration and bioremediation are carried out in this way. Surface and pore channel blockages are removed through physical repairing; chemical remediation parameters are determined according to poisoning types; grading roasting is carried out in thermal regeneration in an inert atmosphere, and parameters are determined according to poisoning degrees; in the bioremediation process, white-rot fungi are used for degrading organic toxicants. A poisoning type identification and evaluation system is established, different poisoning mechanisms are clarified, and a basis is provided for repair. Multiple means are integrated, and targeted, efficient and overall activity recovery is achieved. The method emphasizes grading restoration under precise diagnosis, reduces energy consumption and agent dosage, promotes regeneration to develop towards the environment-friendly, economic and sustainable direction, provides a practical and green scheme for the industry, and assists emission reduction and environmental protection.

The invention relates to the technical field of analysis and detection, and discloses a poison analysis and detection system and method. The poison analysis and detection system comprises a sample processing module which is used for carrying out centrifugal filtration, solid-phase extraction and enrichment and derivatization treatment on a biological sample; the detection module comprises a mass spectrum and high performance liquid chromatography combined system; and the data processing module is used for establishing a database and completing poison detection and contrastive analysis of the sample. The poison analysis and detection system and the poison analysis and detection method have the advantages of high sensitivity, high accuracy and rapid detection, can shorten diagnosis time and accurately guide treatment, and have practical application significance in solving difficult cases.

The application discloses a three-item combined detection kit for methamphetamine analogues in hair, a preparation method and a detection method, and is characterized in that the three-item combined detection kit comprises a quality control disc, a sample tube and a detection working solution, and is used for realizing detection of the methamphetamine analogues; the quality control disc comprises blank quality control, negative control and threshold control; and the detection working solution comprises mobile phase A and mobile phase B. The application relates to the field of forensic science toxic substance analysis, in particular to the three-item combined detection kit for methamphetamine analogues in hair, the preparation method and the detection method. The application aims to provide the three-item combined detection kit for methamphetamine analogues in hair, the preparation method and the detection method, and has the advantages of high detection efficiency, convenient operation and accurate results.

The invention belongs to the technical field of medical treatment, and particularly relates to a dipyridyl poison degradation method and device and application. According to the method, plasma, blood or water and other in-vitro matrixes containing toxicants are irradiated with 190-310 nm ultraviolet light, and efficient degradation of toxicant molecules is achieved; the device comprises an ultraviolet light generation module, the purpose of emitting ultraviolet light is achieved, the wavelength of the ultraviolet light is accurately controlled to be 190-310 nm, and the method and the device provide an efficient and safe technical scheme for in-vitro degradation of dipyridyl toxicants (such as diquat and paraquat). When the device is applied to preparation of a dipyridyl poison detoxification system, an in-vitro poison removal system can be formed, the problem that an existing blood purification technology is low in removal efficiency is solved, the poison removal efficiency is improved, and the poison removal effect after poisoning is improved.