67 results about "Toxicant" patented technology

The invention belongs to the field of biological medicines, and particularly relates to application of FeTMPyP in preparation of a medicine for relieving ovarian toxicity. FeTMPyP is selected as an intervention agent aiming at the fact that MC-LR and NaNON combined exposure can remarkably induce mouse ovary nitration stress, mouse follicle barrier damage and ovarian reproductive endocrine dysfunction; the FeTMPyP can be used for effectively relieving nitration stress induced by combined exposure of MC-LR and NaNO2 in mouse ovaries, improving sex hormone disorder, disordered estrus cycle and follicular atresia phenomena caused by combined exposure of the two poisons, and recovering the fertility of the mouse, so that the intervention of the FeTMPyP can be used for relieving the mouse ovarian toxicity induced by combined exposure of MC-LR and NaNO2, and can be used for treating the ovarian toxicity of the mouse caused by combined exposure of the MC-LR and NaNO2. The compound can be used for preparing medicines for preventing and treating related diseases such as mouse ovarian toxicity caused by environmental toxin exposure, and a new way is provided for early recognition and targeted intervention of ovarian related diseases.

1. Name of the product in this design: Rapid Detection Kit for Toxic Substances. 2. Purpose of this design: This design is used for the rapid detection of toxic substances. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This invention discloses a method for detecting the toxicity threshold of a substance based on a rat drinking experiment. The method of this invention includes the following steps: First, a drinking preference experiment is conducted, and then the toxicity threshold is determined: the amount of substance water and pure water consumed in the drinking preference is compared to determine the time when the rat begins to significantly avoid substance water, the mass of the toxic substance in the rat's body at that time is calculated, and the toxicity threshold of the rat is calculated; (3) dose conversion from rat to human: based on the interspecies dose conversion of body surface area, the toxicity threshold of the rat is first converted into the equivalent dose for humans to obtain the acute exposure dose, and then the acute exposure dose is converted into the chronic exposure dose for humans to obtain the substance toxicity threshold. This invention can accurately determine the toxicity threshold: it achieves toxicity assessment of low-dose exposure through spontaneous recognition behavior; it is easy to operate; and it is applicable to the toxicity assessment and non-destructive threshold determination of various substances such as environmental pollutants, chemical substances, and drugs.

The application provides a toxicant diffusion simulation method based on Fluent, which comprises the following steps: data input, receiving explosion data; first simulation, explosion dispersion simulation according to the explosion data; second simulation, cloud diffusion simulation according to the explosion data and the first simulation result, to obtain a diffusion model. In the application, the concentration and diffusion of toxic substances in a unit space within a short time after the explosion are measured and estimated according to the diffusion model, the explosion process can be simulated and modeled according to the initial explosion data, the radial velocity of explosion diffusion, the concentration of toxic substances, and the size, range, temperature field and pressure field effect of the formed aerosol cloud are obtained, the effective assistance of rescue work under the explosion situation is realized, and the exclusion work or optimization of the space layout of the flammable and explosive scene is realized to improve the safety factor of the scene.

The present invention discloses an environmental pollution monitoring method and device based on an evaluation model, relating to the field of environmental pollution monitoring. The method includes: analyzing the environmental pollution evaluation features of the evaluation model and establishing a multi-dimensional feature list of the evaluation model; analyzing the existence form, diffusion mode, pollution target, and pollution property of the environmental pollution poison monitoring target to obtain the pollution poison monitoring features, and performing a monitoring evaluation target response analysis to obtain the response evaluation demand parameters; using the response evaluation demand parameters to perform a traversal match with the model evaluation features to obtain the missing demand parameters; performing additional training on the evaluation model to obtain an enhanced evaluation model; using the enhanced evaluation model to perform pollutant monitoring and evaluation on environmental monitoring data to obtain the environmental pollution monitoring and evaluation results, and performing pollution monitoring feedback. It solves the technical problem of insufficient accuracy and reliability of the evaluation results in existing environmental pollution monitoring, and achieves the technical effect of improving the accuracy and reliability of the evaluation results.

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

Termite trailing and recruitment compositions, systems, devices, and methods using a trail of a percentage 2 phenoxyethanol (2-PE) dissolved in water to lead subterranean termites to termite bait or monitoring stations with toxicants located about a perimeter of a wood house structures, trees, wood piles, wood pallets, and wood boats. The percentage 2 phenoxyethanol (2-PE) dissolved in water range from approximately 0.1% to approximately 2% depending on soil types. Devices and methods form a complete barrier about wood members so termites are redirected due to elevated soil water concentrations that connect termite toxicants, monitoring stations and / or bait stations with toxicants. Devices and methods form trails of 2-PE dissolved in water direct termites to termite monitoring stations and / or bait stations with toxicants, located on wood-based house structures, trees, boats, cut wood, wood pallets, fences, posts. Trails of 2-PE dissolved in water surrounding termite monitoring stations / bait stations with toxicants can direct termites to the termite toxicants.

The Cardio-Tox Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The invention discloses a diagnostic model construction method based on a novel network toxicology research normal form, and belongs to the field of health risk assess.The diagnostic model construction method specifically comprises the following steps that firstly, a potential poison and disease target library is constructed, and common potential targets between ATBC and fracture healing damage are screened and recognized; iI, constructing a PPI network to identify a core target, and analyzing functions and pathway enrichment of the core target; iII, identifying and screening the biomarkers, and constructing a diagnostic model to evaluate the diagnostic value of the biomarkers; according to the method, the evaluation precision of ATBC on the potential risk of fracture healing is remarkably improved, the conformation limitation of traditional static docking is broken through, stability evaluation in the time dimension is provided, the limitation of a rigid docking method is effectively overcome, meanwhile, visual representation of the dynamic behavior of a compound is provided, and the accuracy of the dynamic behavior of the compound is improved. The method provides a quantitative basis for mechanism analysis of molecular interaction, provides an effective technical means for subsequent high-risk substance screening, and is simple and convenient to operate and wide in application range.

The invention relates to the field of biological control, in particular to an environment response type African giant snail capsule attractive toxicant and a preparation method and application of the environment response type African giant snail capsule attractive toxicant. The capsule attractant comprises a capsule shell and a composition located in the capsule shell, and the composition is prepared from, by mass, 5%-10% of metaldehyde, 50%-65% of attractant, 30%-40% of macromolecule water-absorbent resin, 0.1%-1% of mildew preventive, 0.1%-1% of preservative and 0.1%-1% of coloring agent. The capsule attractant provided by the invention is ingeniously arranged according to the specific life habits of the African snails, and through various exquisite arrangements of a capsule shell, an internal mildew-proof system, SAP water absorption expansion and water locking, the validity period of the attractant can be remarkably delayed, and the pesticide effect can be highly synchronized with the emergence peak of the African snails; the damage to non-target organisms is greatly reduced through optimal setting of the capsule, and the ecological friendliness is outstanding.

The present application belongs to the field of biological toxicity monitoring and ecological toxicity detection, and particularly relates to a pollutant exposure device suitable for aquatic organisms and a large-scale simulation continuous dilution method. The device comprises a cylinder with an open top, a buffer zone, a toxicant exposure zone and a water outlet zone arranged in sequence in the cylinder, the buffer zone and the toxicant exposure zone, and the toxicant exposure zone and the water outlet zone are separated by a partition, the partition is perforated, the buffer zone, the toxicant exposure zone and the water outlet zone are provided with an internal circulation system, and the internal circulation system comprises a submersible pump, a circulating water pipe and an aeration assembly. The method simulates the condition that a real water body receives sewage by using the device, the dispersion and dilution of pollutants are achieved by water flow and aeration, the circulation of pollutants and water bodies is achieved by a circulating system, the water inlet flow of the device is achieved by water containing pollutants, the real concentration that organisms can contact is simulated, and the method has strong application prospects in the fields of non-destructive online monitoring of sewage and wastewater and toxicity simulation of pollutants entering large-scale water bodies.

The disclosures herein relate to cucurbituril compounds and methods and compositions for reducing the amount of a toxin in a patient by administering to the patient an effective amount of the cucurbituril compound. Also provided are methods and compositions for preventing or treating an overdose from a toxin that has been ingested by a patient.

The invention discloses a JNK recombinant protein as well as a preparation method and application thereof, and relates to the technical field of recombinant protein preparation and purification. The preparation method comprises the following steps: S1, taking cDNA (complementary deoxyribonucleic acid) of cristata plicata as a template, and carrying out PCR (polymerase chain reaction) amplification by adopting a specific primer to obtain an amplification product; s2, connecting the amplification product to a carrier by using a homologous recombination technology to construct a recombinant plasmid; and S3, carrying out induced expression on the recombinant plasmid, and purifying to obtain the JNK recombinant protein. The JNK recombinant protein is prepared, the efficient preparation system conditions of the JNK protein are optimized, the target protein is captured through a nickel column affinity chromatography method, the protection effect of the JNK protein in cell resistance toxicants and the resistance of the JNK protein to H2O2 are evaluated, key data support is provided for subsequent research on development of the efficacy of the JNK protein, and the JNK protein has good application prospects. Recycling of shellfish processing waste is promoted, and experimental reference is provided for deep research and development of shellfish active substances.

The invention relates to a method for simultaneously detecting multiple amatoxin, and relates to the technical field of analysis and detection, and the method comprises the following steps: S1, preparing a sample solution to be detected; s2, detecting the amatoxin in the sample solution to be detected by adopting a liquid chromatography-mass spectrometry method; wherein the chromatographic separation conditions of the liquid chromatography-mass spectrometry method are as follows: a chromatographic column: Kinetex is 2.6 [mu] m XB-C18 100A, and LC Column is 50 * 3 mm; mobile phases: the phase A is formic acid water, the phase B is methanol, and gradient elution is carried out. According to the application, synchronous detection of four target objects (alpha-amanita, beta-amanita, gamma-amanita and dihydroxy sticktight) can be completed within a short time, the sensitivity is very high, the requirement of poison residue analysis can be met, and a powerful technical support is provided for emergency detection of public health emergencies.

The invention discloses a toxicity risk evaluation method for low-concentration composite pollutants. The method comprises the following steps: placing gill cells in a filtered sewage sample for exposure contamination; the method comprises the following steps: marking cell structures of gill cells by using a mixed fluorescent staining agent, and carrying out high-throughput shooting on stained and marked cell images by using a high-content automatic imaging analysis system to obtain a multi-parameter phenotype spectrum; unsupervised learning clustering is carried out on the multi-parameter phenotype spectrum to form different clusters to complete dimension reduction, the sewage toxicity risk level is judged through machine learning model training, and a gradient boosting tree is finally selected through a hyper-parameter optimization model to establish a water quality toxicity risk model; inputting the multi-parameter phenotype spectrum into the water quality toxicity risk model, and screening by clustering categories to obtain a toxicity risk grade; the method can effectively and comprehensively evaluate the toxicity risk of the low-concentration composite pollutants in the effluent of the sewage treatment plant, and has practical significance for guiding the water quality risk control of the sewage treatment plant.

The invention provides a pomacea canaliculata egg poisoning reagent and a pomacea canaliculata egg poisoning method, and belongs to the technical field of agricultural pest control. The invention provides a pomacea canaliculata ovum poisoning reagent. The active ingredient of the pomacea canaliculata ovum poisoning reagent comprises an aqueous extract of an oleander raw material; the oleander raw material comprises one or more of leaves, stems and flowers of oleander; the aqueous extract of the oleander raw material contains rutin and oleandrin. The active ingredients of the poisoning agent comprise water-soluble natural active ingredients in the oleander, so that the poisoning agent has the advantage of being environmentally friendly, and the problem that the environment is polluted by a traditional chemical control method is effectively solved. Moreover, the aqueous extract of the oleander raw material contains rutin and oleandrin, and the rutin and the oleandrin have a synergistic effect, so that the poison killing effect on the ampullaria gigas eggs can be remarkably improved, and the problem of low physical prevention and treatment efficiency is solved. The poison reagent provided by the invention is suitable for green and accurate prevention and control of pomacea canaliculata in an agricultural ecosystem, especially a rice field.

Systems and methods for mapping toxicants are disclosed. A toxicant database is generated by preparing data retrieved from multiple data sources. Based on a query, which may include a location, the toxicant database is accessed and a map is generated from the toxicant database using at least the location in the query. A report may be generated that provides remediation and mitigation information. The map and the report are presented in a user interface.

This invention belongs to the field of biomedicine, and specifically relates to the application of FeTMPyP in the preparation of drugs to alleviate ovarian toxicity. This application addresses the issue that combined exposure to MC-LR and NaNO₂ significantly induces nitrification stress in mouse ovaries, leading to follicular barrier disruption and ovarian reproductive endocrine dysfunction. FeTMPyP was selected as an intervention agent, which effectively alleviated nitrification stress induced by combined exposure to MC-LR and NaNO₂ in mouse ovaries, and improved sex hormone disorders, estrous cycle irregularities, and follicular atresia caused by the combined exposure to these two toxins. Simultaneously, it restored the fertility of mice, indicating that FeTMPyP intervention can alleviate ovarian toxicity induced by combined exposure to MC-LR and NaNO₂ in mice. This invention can be used to prepare drugs to prevent and treat diseases related to ovarian toxicity caused by environmental toxin exposure in mice, providing a new approach for the early identification and targeted intervention of ovarian-related diseases.

The invention belongs to the field of rice breeding, and particularly discloses a primer group for detecting a PARMS molecular marker of a rice plant height gene OsPH4 and application of the primer group, dwarf type OsPH4 gene detection is carried out on a rice material through a molecular sequence of the rice plant height gene OsPH4 by applying a PARMS technology, and the rice plant height gene OsPH4 is obtained. The rice dwarf type OsPH4 gene can be rapidly and accurately detected in different germplasm resources such as indica rice and japonica rice. According to the method, tedious procedures such as enzyme digestion, electrophoresis and sequencing are not needed in the detection process, pollution of PCR product aerosol and use of toxic substances such as EB are reduced, meanwhile, foreground selection and background selection can be conducted in the early stage of molecular marker assisted breeding, the background recovery rate is increased, the scale of breeding populations is reduced, the breeding process is accelerated, and the breeding efficiency is improved. The efficient and environment-friendly application of the OsPH4 gene in commercial molecular breeding of rice is facilitated.

The invention aims to provide a method for preparing regulatory T cells and knocking out Stub1 to enhance functions of the regulatory T cells, and belongs to the field of cell preparation. Comprising the following steps: (1) Tregs sorting; (2) carrying out subsequent in vitro amplification on the Tregs; and (3) knocking out Stub1 in the Tregs by using CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Compared with an unedited polyclone Tregs group and an environment poison exposure group, in an environment poison induced immune homeostasis imbalance model, the Stub1 knocked-out Tregs are fed back, so that the immune homeostasis imbalance state of rats is remarkably improved, and tissue damage of immune organs can be effectively repaired. Further detection shows that the Foxp3 protein level in the Tregs in the group of rats is remarkably increased, and meanwhile, the number of Tregs cells is also maintained at a relatively high level. The results prove that the strategy provided by the invention has a remarkable intervention effect on improving immune organ injury in the aspects of enhancing the Tregs inhibition function and maintaining immune balance.

The invention discloses a construction method and application of a sperm-specific hOdf2-GFP transgenic mouse model, and relates to the technical field of reproductive biology and transgenic animal model construction. According to the construction method disclosed by the invention, based on a piggyBac transposon expression vector system, a DNA (Deoxyribose Nucleic Acid) sequence containing a coding human source Odf2 is effectively connected with a Protamine1 promoter. The transgenic positive mouse constructed by the method can specifically express human Odf2, has a dynamic change from sperm to zygote to eight cell stages, realizes early continuous visual tracking of embryos, has genetic stability, keeps expression and phenotype consistency across generations, and has a good application prospect. The method can be applied to the fields of environmental poison evaluation, asthenospermia mechanisms, drug screening and the like.

The Cardio-Tox Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The utility model provides a storage device convenient to check forensic poison acceptance samples, which relates to the technical field of sample storage devices and comprises a machine body, a bottom plate is arranged in the machine body, a vertical column is fixed at the top end of the bottom plate, and a plurality of first telescopic rods are mounted on the outer wall of the vertical column at equal intervals. A storage block is fixed to the output end of each first telescopic rod, and three storage holes are formed in the top end of each storage block. Through the arrangement of a plurality of storage blocks, each sample is stored in the storage blocks, when the sample needs to be taken out, the whole bottom plate is driven to rotate through the operation of a motor, the corresponding storage blocks are moved to a discharging pipe, then the single storage block is driven to move through the operation of a first telescopic rod, and the storage blocks are moved out of the discharging pipe; by arranging the discharging pipe, the problem that a large amount of cold air leaks due to the fact that a top cover in a traditional device is opened can be avoided, and the practicability is higher.

The present invention discloses a method for calculating the nuclear density of poisons in fuel spheres circulating outside the reactor of a pebble-bed high-temperature gas-cooled reactor. First, the distribution of multiple neutron fluxes within the reactor is obtained; the burnup chain of important poisons is simplified, and the burnup equation of the simplified burnup chain is established and solved to obtain the equilibrium concentration of the batch that will return to the reactor core in all areas of the bottom layer of the pebble bed at the current stage, i.e., the initial fuel sphere poison concentration; a burnup equation is established after the fuel spheres are discharged from the reactor, and the nuclear density of a batch of poisons discharged from a flow channel, after staying outside the reactor for a period of time before returning to the reactor core, is derived and calculated; until the change in the nuclear density of poisons in each flow channel that will return to the reactor core is calculated; the nuclear density of the same batch of poisons in different flow channels that have decayed outside the reactor for a period of time is calculated as a volume-weighted average based on the area ratio of each flow channel; and finally, the nuclear density of the poisons in the batch that returns to the reactor core after decaying outside the reactor and being evenly mixed is obtained. The method of the present invention makes the poison concentration of the fuel spheres that have returned to the reactor core after circulating outside the reactor more reasonable.

The invention discloses a miniature device for rapidly and directly detecting a poison sample and a use method of the miniature device, and relates to the technical field of analytical chemistry and biosensors.The miniature device comprises a magnetic enrichment unit, a magnetic recognition probe and a signal probe. A magnetic enrichment unit is utilized to enrich a magnetic recognition probe combined with a target object in a micro-droplet at the top end, then a signal probe is added, qualitative and quantitative analysis of the target object is achieved by detecting a generated fluorescence signal, and the specific magnetic recognition probe does not need sample pretreatment; the magnetic recognition probe and the signal probe are combined to realize ultrahigh-sensitivity detection; all detection steps are integrated in the micro-droplet at the top end of the magnetic bar, so that the device is extremely miniaturized and portable, reagent consumption and cost are reduced, the device solves the problems of complex sample pretreatment, serious matrix interference and the like, and miniaturized and low-cost field rapid detection of high-sensitivity and high-specificity detection is realized.

The disclosures herein relate to cucurbituril compounds and methods and compositions for reducing the amount of a toxin in a patient by administering to the patient an effective amount of the cucurbituril compound. Also provided are methods and compositions for preventing or treating an overdose from a toxin that has been ingested by a patient.

The invention provides a preparation method of hydroxycotinin-D3, and aims to solve the problem that existing hydroxycotinin-D3 lacks a reference substance and a deuterated substance and hinders forensic poison identification, 3-(pyrrolidine-2-yl) pyridine is used as a raw material, a product is prepared through deuterium methylation, oxidation, hydroxylation and refining, a final finished product is colorless needle-shaped crystals, and after the colorless needle-shaped crystals are dried at 45 DEG C for 72 hours, the hydroxycotinin-D3 is obtained. According to the present invention, the yield is 56% based on the crude product, the HPLC detection results show that the purity is more than 99.7%, the single impurity content is less than 0.1%, the total impurity content is less than 0.3%, the whole product has high stability and high reaction specificity from the intermediate to the finished product, the final finished product completely meets the strict requirements of the reference substance for forensic poison identification, the prepared hydroxycotinin-D3 has advantages of high reaction efficiency, good product quality and the like.

A method of manufacture and a fabric product that includes a concentrated formulation of photosensitizers having anti-microbial properties that are applied to outer surfaces of the fabric product. The inner surfaces of the fabric product are configured as a protective barrier for a user of the fabric product. Light activation of the photosensitizers protects the user against a range of pathogens, toxins, poisons, microbial particles and radiation particles incident on the outer surfaces of the fabric product.