141 results about "Follicle" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention discloses a method and a system for automatically identifying primordial follicles in an ovary microscopic section image, which are used for realizing automatic identification and counting of primordial follicles in the microscopic image by carrying out image binaryzation, expansion corrosion processing, edge detection, circular feature extraction and texture feature analysis on the ovary microscopic section image dyed under a microscope. According to the method, a local binary pattern (LBP) is adopted to extract texture features, and primordial follicles in a microscopic slice image are effectively distinguished by combining edge detection and a hollow circular screening algorithm; the method has the characteristics of high processing speed, high precision and strong adaptability, and can be widely applied to ovarian slice analysis, assisted reproductive medicine and research of ovarian function related diseases.

Described herein are compositions for promoting hair growth and / or treating or preventing hair loss in a mammalian subject. In certain embodiments, the mammalian subject is a human male or a human female. Methods of making and using the same are also described. The compositions in various embodiments may comprise: a tea extract which may comprise a polyphenol, e.g., epigallocatechin gallate; an extract from Larix europaea which may comprise the flavonoid taxifolin; a natural ingredient that antagonizes or mitigates the proliferation of Gram-positive bacteria in the hair shaft passageway; compounds including zinc chloride and / or hexamidine diisethionate; follicle stimulator. e.g., caffeine, an ingredient that improves the health of the dermis. e.g., sodium hyaluronate; and / or ingredients specifically tailored for male or female subjects, e.g., Serenoa serrulata fruit extract or extract of Pterocarpus marsupium.

The invention discloses a traditional Chinese medicine composition for repairing and / or activating residual ovarian functions of an aging ovary, and a preparation method and application thereof, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: radix astragali, radix angelicae sinensis, radix ophiopogonis, radix polygonati officinalis, semen cuscutae, radix morindae officinalis, fructus rubi, rhizoma dioscoreae and folium mori. The invention further provides application of the traditional Chinese medicine composition in repairing and / or activating the residual ovarian function of the senescent ovary and improving the quality of the senescent oocyte, the traditional Chinese medicine composition can increase the volume and weight of the senescent ovary, increase the number of follicles of all levels in the senescent ovary, reduce apoptosis of granular cells in the ovary and improve the survival rate of the senescent ovary. The abnormal rate of oocytes, oxidative stress of the oocytes and DNA damage are reduced, development and maturation of residual follicles are promoted and maintained, and the traditional Chinese medicine composition has a wide application prospect.

The invention relates to the technical field of precise operation positioning, in particular to an ultrasonic monitoring and guiding method in a follicle puncture ovum taking process, which comprises the following steps of: analyzing a movement stability condition and a path deviation condition of an ultrasonic probe in a movement process to obtain a path deviation rate, and utilizing distribution characteristics of gray level and gradient level in an ultrasonic image to obtain a path deviation rate; the air interference degree of the ultrasonic image is obtained according to the path deviation rate, the echo signal intensity difference of the ultrasonic probe under the adjacent frames and the tissue contrast difference of the ultrasonic image, the information misunderstanding degree of the ultrasonic image is obtained through the path deviation rate and the air interference degree, and the attenuation characteristic of the ultrasonic echo signal is combined to obtain the information misunderstanding degree of the ultrasonic image. Gain compensation factors of echo signals of the ultrasonic probe are obtained, the echo signals are enhanced, and path guiding is conducted on the follicle puncture ovum taking process through an ultrasonic image obtained after the echo signals are enhanced. According to the method, the enhancement effect on the ultrasonic image in the follicle puncture process is effectively improved.

The invention discloses a multi-factor risk assessment method for ovarian hyperstimulation, and relates to the technical field of intelligent medical treatment, and the method comprises the steps: based on a multi-source baseline data packet, calculating the trend of follicle volume along with time through ovarian reaction simulation to obtain a follicle reaction curve, calculating the probability of vascular injury through puncture path bleeding simulation, and calculating the risk of ovarian hyperstimulation according to the probability of vascular injury. Obtaining a bleeding probability, and fusing the follicle reaction curve and the bleeding probability to form a patient characterization risk vector; associating the intervention suggestions in the structured strategy list with the ovarian overstimulation risk score and the bleeding risk score to generate a risk change description, and forming a risk assessment report according to the risk change description; according to the method, the patient characterization risk vector fusing the follicle dynamic response and the puncture bleeding risk is constructed, so that the safety and individuation level of assisted reproduction treatment are effectively enhanced.

The invention discloses an intelligent precise positioning system for hair follicles in micro-needle art hair transplantation, and relates to the technical field of image processing of hair follicles in hair transplantation. According to the system, a to-be-transplanted hair area is determined through an obtained hair follicle three-dimensional network image, a two-dimensional coordinate system taking a to-be-measured target area as a reference is established to carry out preliminary planting density planning, then hair follicle density gradient division is carried out according to the determined to-be-transplanted hair area, and the coordinate position of each hair follicle is marked; a visual hair follicle planting point location distribution map is formed, a reference point location is provided for positioning of the microneedle, finally, skin deformation quantity and microneedle positioning coordinate deviation are monitored in real time in a spatial feature coordinate system, microneedle puncture positioning is adjusted, accurate positioning alignment of the microneedle and a target hair follicle is achieved, and the accuracy of the positioning of the microneedle and the target hair follicle is improved. The problem that in the prior art, when a microneedle carries out planting operation according to feedback information, in the link that position image coordinates of a to-be-planted area are converted to a mechanical arm coordinate system, the intelligent positioning accuracy of hair follicles is not high is solved.

The present invention belongs to the field of natural compounds, and specifically relates to a crude extract of frog follicles, the VMO1 protein, and their extraction methods and uses. The specific technical solution is as follows: A crude extract, wherein the crude extract is from frog follicles and does not contain egg grains. The preparation method includes: extracting the follicles of frogs, removing the egg grains under a sterile environment, adding a buffer solution, performing ultrasonic treatment and centrifugation, taking the supernatant, and filtering to obtain the crude extract. The crude extract obtained by the present invention has good maintained activity and can be further developed for subsequent uses. The crude extract obtained by the present invention can significantly reduce the surface tension, and at the same time has good antioxidant activity and good antibacterial ability, such as the ability to inhibit Pseudomonas aeruginosa. Since products derived from natural products have good biocompatibility, therefore, based on the above activities and functions, the crude extract provided by the present invention has good application prospects in multiple fields such as the field of cosmetics water retention, the field of food preservation, and the field of pharmaceuticals.

The invention provides application of a traditional Chinese medicine composition in preparation of a medicine for preventing and / or treating ovarian dysfunction, and belongs to the technical field of biological medicine. The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 8-10 parts of American ginseng, 2-4 parts of pseudo-ginseng, 55-65 parts of kudzuvine root, 42-48 parts of coptis chinensis, 42-48 parts of scutellaria baicalensis, 14-16 parts of stiff silkworm, 14-16 parts of rhizoma zingiberis, 42-48 parts of bitter gourd and 55-65 parts of rhizoma anemarrhenae. The traditional Chinese medicine composition has the effects of increasing the number of follicles and corpus luteum, restoring the duration of an estrus cycle and restoring the levels of serum hormones and sex hormone receptors. The traditional Chinese medicine composition provided by the invention provides a new means for preventing and treating ovarian dysfunction.

The invention discloses a traditional Chinese medicine composition, a traditional Chinese medicine extract, a pharmaceutical preparation and application for treating hypoovarian reserve function. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 12 to 18 parts of radix astragali seu hedysari, 9 to 15 parts of radix angelicae sinensis, 9 to 15 parts of fructus ligustri lucidi, 9 to 15 parts of fructus mori, 9 to 15 parts of semen cuscutae, 6 to 12 parts of cornu cervi degelatinatum, 7 to 13 parts of tortoise shell and 7 to 13 parts of radix cyathulae. The traditional Chinese medicine composition, the traditional Chinese medicine extract and the pharmaceutical preparation are low in cost and high in safety, and can remarkably improve the ovarian function of an elderly patient suffering from hypoovarian reserve function, increase the number of sinus follicles and improve the follicle quality.

The invention relates to the technical field of pig feeds, and particularly discloses a special compound feed for weaning sows, the feed comprises a functional additive, the functional additive comprises the following raw materials in parts by weight: 1-3 parts of a plant extract compound, 2-5 parts of conjugated linoleic acid, 0.0003-0.0005 part of selenomethionine, 0.15-0.35 part of vitamin E, and 0.15-0.3 part of vitamin C; the plant extract compound is prepared from the following components: astragalus polysaccharide, icariin, epigallocatechin gallate and a carrier, and the mass ratio of the astragalus polysaccharide to the icariin to the epigallocatechin gallate is 1 to (1 to 4) to (0.2 to 0.8). The special compound feed for the weaned sows is used for feeding the weaned sows through a scientific and reasonable formula, can promote follicular development of the sows after weaning, improve the oestrus rate and reduce the proportion of non-oestrus sows, and can solve the problem of slow ovarian function recovery of the sows in summer high-temperature seasons.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The utility model relates to the technical field of hoisting equipment, and is an anti-mistake device capable of preventing a large hook of a crane from rotating, comprising a support frame, and also comprising: a shell fixed to the inner wall of the support frame, a winding roller is arranged inside the shell, a hoisting rope is arranged on the outer side of the winding roller, a connecting frame is fixedly connected to the bottom of the shell, a hook is arranged at the bottom of the connecting frame, and a limiting member is arranged on one side of the connecting frame; a box body fixed to the bottom of the support frame, a guide assembly is arranged inside the box body; the utility model can drive the support frame to be hoisted and lowered through the winding roller and the hoisting rope, and the effect of limiting the hook is achieved by setting the limiting member, so as to avoid the problem of aluminum rod collision caused by natural rotation of the hook, thereby improving the safety of use, and the effect of guiding the hook when working in a deep well is achieved by setting the guide assembly, thereby improving the hoisting stability.

The utility model discloses a follicle hatching device for fry cultivation, relates to the technical field of follicle hatching, and aims to solve the technical problem that a current follicle hatching device easily causes adhesion of fish follicles to cause hatching failure. The follicle hatching device comprises a culture box, a shunt tube is arranged at the top of the culture box, and a mounting plate is arranged in the culture box; a plurality of groups of hatching barrels are movably arranged on the mounting plate. By designing the structures of the hatching barrel, the mounting pipe, the flow dividing pipe, the connecting hose, the first water pump, the second water pump and the like, after a water source is discharged into the hatching barrel from the center of the bottom in the hatching barrel through the mounting pipe, the water source in the hatching barrel is in a surging-shaped flow generation state at the moment, so that follicles can upwards surge according to water flow, and follicles in the hatching barrel can be more effectively hatched. The follicles can be dispersed, the fish follicles can be prevented from being adhered together, the environment in a parent body can be simulated through proper gushing, and development and incubation of the fish follicles can be better promoted.

The invention discloses application of paeonol in preparation of a product for improving liver functions and ovarian functions of laying hens in the later egg laying period, belongs to the technical field of feed additives, and particularly relates to application of paeonol in preparation of a product for improving liver functions and ovarian functions of laying hens in the later egg laying period. It is found that paeonol can significantly reduce the activity of glutamic-pyruvic transaminase in the liver, significantly reduce the content of cholesterol and the content of triglyceride in serum, significantly reduce hepatic cell fatty degeneration and lipid droplet aggregation in a hepatic tissue pathological section, relieve liver injury and lipid metabolism disorder, and significantly improve the liver function. The paeonol can effectively improve the fatty liver hemorrhagic syndrome of the laying hens in the later egg laying period, and the paeonol can recover the storage capacity of ovary by improving the survival rate of follicles, improve the egg laying performance of the laying hens and improve the egg laying rate.

The invention discloses a method for promoting ovulation of sexual maturity zebrafish by using Gap27, and relates to the technical field of artificial breeding of fish, a gap connecting protein inhibitor Gap27 is applied to ovulation induction of zebrafish, an intercellular communication network in follicles is accurately intervened, the problem of asynchronous ovulation is solved, and in the final stage of maturation of oocytes, ovulation of sexual maturity zebrafish is promoted. Close substance and information exchange is carried out among follicular granule cells through a gap connecting channel formed by connecting protein Cx43, and Gap27 is used as a specific peptide fragment, can simulate an extracellular ring structure of Cx43, and competitively binds with Cx43 on an adjacent cell membrane, so that formation and functions of the gap connecting channel are physically blocked, and the follicular granule cells are separated from each other. When ovarian tissues of sexually mature female fish are exposed in a Gap27 solution, the transmission of inhibitory signals from granular cells to oocytes can be effectively interrupted, the rupture of nuclear membranes of the cells is promoted, and the final maturation process is completed.

The invention discloses application of spermidine in preparation of a preparation for improving fertility of female mammals, and belongs to the technical field of reproductive biology. According to the invention, spermidine with different concentrations is introduced into mice, and the effect of spermidine on ovarian hormone secretion, ovarian reserve level and follicle number of female mammals is determined in a diet intake manner, so that the fertility of female animals is improved; it is found that spermidine can effectively improve the ovarian function, increase the secretion level of E2, P4, LH and AMH hormones closely related to the ovarian function, reduce the number of atresia follicles of the ovarian and increase the litter birth number of female mice by 1-2, and the spermidine has good animal breeding and livestock raising application prospects.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

This invention relates to the field of ovarian tissue in vitro culture technology, and discloses a formulation for activating and increasing follicles and its application. The formulation consists of a PI3K / Akt / mTOR pathway activator and / or a PTEN inhibitor SF1670. The PI3K / Akt / mTOR pathway activator is a 5-15 μM statin, and the PTEN inhibitor is a 20-50 μM SF1670. Using this formulation can achieve the follicle activation and increase effect of existing technologies, contributing to the development and selection of in vitro follicle activators in clinical practice. Furthermore, the use of this formulation opens up a new technological application area for statins.

The utility model provides a follicle flushing liquid constant-temperature device which comprises a constant-temperature box, a box body cover plate is rotationally connected to the constant-temperature box, a fixing opening is fixedly connected to the box body cover plate, the fixing opening is connected with a flushing assembly, an extrusion assembly is arranged in the constant-temperature box, and the extrusion assembly is connected with a constant-temperature assembly. The extrusion assembly achieves the stable and continuous flushing effect and controls the flushing force, the constant-temperature assembly achieves uniform dispersion of heat, so that flushing liquid can be continuously heated, the temperature sensor achieves temperature regulation and control, the constant-temperature state of the flushing liquid is achieved, and the flushing assembly guarantees sterility of medical supplies in contact with the flushing liquid. And control over the flushing flow can be achieved through the arranged flow adjusting valve.

The present application relates to a kind of two-dimensional metal polyphenol complex nanomaterials that can be enriched in ovary, preparation method and application thereof, the nanomaterial can be enriched in ovary, including two-dimensional metal polyphenol complex as substrate, and follicle-stimulating hormone polypeptide grafted to the surface of the substrate;Wherein, the surface of the two-dimensional metal polyphenol complex grafts-COOH functional group and forms 2D MPC-COOH intermediate, the follicle-stimulating hormone polypeptide is covalently grafted with the-COOH on the surface of 2D MPC-COOH intermediate and obtains the two-dimensional metal polyphenol complex nanomaterial.The technical effect of the present application is that the preparation method of nanomaterial is simple, and nanoparticle dispersibility is good, and biosafety is high, and nanomaterial can physically absorb BRD7 target protein through its special structure, block BRD7 into cell nucleus, and then realize the safe down-regulation of KAT7 path and the effective reversal of granulosa cell stress aging.

The application relates to the field of biotechnology, and particularly relates to an application of Bacteroides ovatus in ovarian aging; the application comprises: taking low-abundance Bacteroides ovatus as a biomarker of ovarian aging; the application comprises using Bacteroides ovatus in a medicine for delaying ovarian aging; the application further comprises using a fermentation product of Bacteroides ovatus in the medicine for delaying ovarian aging; it is found through experiments that there is a significant difference in Bacteroides ovatus in mice with different degrees of ovarian aging, and it is found through subsequent experiments that Bacteroides ovatus has the effects of significantly increasing the number of oocytes, up-regulating anti-Mullerian hormone, up-regulating the level of estradiol and down-regulating the level of follicle-stimulating hormone, which indicates that Bacteroides ovatus can be used in the medicine for delaying ovarian aging.

The invention discloses an additive for resisting cow ovarian aging and its application. The additive is made of a composition of anthocyanidins, glycyrrhizic acid and wolfberry glycopeptide. When the composition is used in clinical veterinary treatment of cow ovarian aging, the role of primary cow ovarian granulosa cells is clarified through transcription, which is mainly involved in regulating programmed cell death, Ras protein signal transduction, tissue development, cell serine, tryptophan kinase activity, MAPK signaling pathway, PI3K-AKT signaling pathway, and laser confocal results show that the composition can significantly reduce the production of mitochondrial reactive oxygen; at the same time, the therapeutic effect on the persistent corpus luteum of the main cow is achieved by improving the intestinal flora structure, and more specifically, the colony is significantly changed upstream at the genus and species level; B-ultrasound results reveal that for a persistent corpus luteum cow, more large follicles can be detected on the ovaries after one month of gavage with the additive composition.

Disclosed are a method and culture medium for performing an in vivo treatment on ovaries of mammals including humans or performing in vitro culturing on ex vivo ovaries or ovarian tissue by means of using 4-vinylcyclohexene diepoxide. The method or culture medium provided facilitates the retention and enhancement of the developmental and reproductive potential of mammalian ovaries or ovarian tissue, and is particularly capable of promoting follicle maturation and increasing the number of ovulations.

The application discloses a superovulation method based on synergistic effect of composite hormones, which comprises the following steps: injecting PMSG and FSH to induce estrus, and injecting hCG to promote synchronous ovulation. The application adopts synergistic regulation of PMSG (long-acting follicle-stimulating hormone) and FSH (short-acting follicle-stimulating hormone), PMSG completes follicle recruitment, and FSH finely regulates the development stage, thereby avoiding overgrowth of follicles. In the examples, the Burmese cat, the British short cat and the farm cat do not appear OHSS symptoms (such as ovarian enlargement and ascites), and the ovarian state is normal.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to a composition for improving premature ovarian failure and a preparation method thereof. The composition comprises the following components in parts by weight: 5-15 parts of orange osmanthus pollen, 4-12 parts of flaxseed concentrated powder, 2-8 parts of black-bone chicken peptide, 4-20 parts of rehmannia powder, 2-10 parts of cistanche powder and 1-5 parts of lemon concentrated powder. The composition for improving premature ovarian failure prepared by the invention can reduce the content of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the serum of a premature ovarian failure rat induced by cyclophosphamide and increase the content of estradiol (E2), thereby achieving the effect of improving premature ovarian failure.

The present invention relates to methods of rejuvenating aged oocytes using young somatic cells through the generation of chimeric follicles. The chimeric follicles defined herein may be used to treat infertility or improve fertility in female subjects.

The invention discloses a live sow egg collecting instrument system, and belongs to the technical field of livestock breeding detection. A living sow egg collecting instrument system comprises a host, an ultrasonic probe, a negative pressure pump, an egg collecting tube, an egg collecting needle and an operation system. The host is a control center of the system, integrates an ultrasonic signal processing module, a negative pressure adjusting module and a data recording module, and is used for receiving signals transmitted by the ultrasonic probe, adjusting the pressure of the negative pressure pump and recording key data in the egg collecting process. The ultrasonic probe is electrically connected with the host, is used for transmitting and receiving ultrasonic signals and is inserted into the dome part of the vagina of the sow, and after the operation system is started, the probe is in contact with ultrasonic images of ovaries and follicles of the sow and displays the ultrasonic images on a host screen; the problems that in the prior art, the utilization rate of improved variety resources is low, the egg collecting accuracy and efficiency are insufficient, the egg quality is not guaranteed, and data management is disordered cannot be effectively solved.

A sensor comprising a whisker shaft and a follicle is provided. The shaft has a root end and a tip end and the shaft tapers from the root end to the tip end so that the root end is wider and the tip end is narrower. The root end is pivotably mounted in the follicle.

This invention discloses a Polygonatum sibiricum vesicle preparation for treating premature ovarian failure, its preparation method, and its application. The preparation method includes: cleaning Polygonatum sibiricum, juicing, filtering to remove residue, and collecting the juice; subjecting the juice to gradient centrifugation to remove residual plant tissue, plant cells, and plant cell debris; then using an extraction reagent for stepwise precipitation, collecting the precipitate, and resuspending it to obtain the final product. The Polygonatum sibiricum vesicle preparation prepared by this invention can be efficiently taken up by ovarian granulosa cells. It effectively treats premature ovarian failure by activating the Nrf2 signaling pathway, upregulating antioxidant enzyme expression, scavenging reactive oxygen species, restoring mitochondrial membrane potential, inhibiting apoptosis, and restoring the expression levels of estrogen, follicle-stimulating hormone, and anti-Müllerian hormone. The preparation method of this invention is standardized, biocompatible, and safe, and has broad clinical application prospects.