74 results about "Follicle" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention discloses a multi-factor risk assessment method for ovarian hyperstimulation, and relates to the technical field of intelligent medical treatment, and the method comprises the steps: based on a multi-source baseline data packet, calculating the trend of follicle volume along with time through ovarian reaction simulation to obtain a follicle reaction curve, calculating the probability of vascular injury through puncture path bleeding simulation, and calculating the risk of ovarian hyperstimulation according to the probability of vascular injury. Obtaining a bleeding probability, and fusing the follicle reaction curve and the bleeding probability to form a patient characterization risk vector; associating the intervention suggestions in the structured strategy list with the ovarian overstimulation risk score and the bleeding risk score to generate a risk change description, and forming a risk assessment report according to the risk change description; according to the method, the patient characterization risk vector fusing the follicle dynamic response and the puncture bleeding risk is constructed, so that the safety and individuation level of assisted reproduction treatment are effectively enhanced.

The invention discloses a traditional Chinese medicine composition, a traditional Chinese medicine extract, a pharmaceutical preparation and application for treating hypoovarian reserve function. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 12 to 18 parts of radix astragali seu hedysari, 9 to 15 parts of radix angelicae sinensis, 9 to 15 parts of fructus ligustri lucidi, 9 to 15 parts of fructus mori, 9 to 15 parts of semen cuscutae, 6 to 12 parts of cornu cervi degelatinatum, 7 to 13 parts of tortoise shell and 7 to 13 parts of radix cyathulae. The traditional Chinese medicine composition, the traditional Chinese medicine extract and the pharmaceutical preparation are low in cost and high in safety, and can remarkably improve the ovarian function of an elderly patient suffering from hypoovarian reserve function, increase the number of sinus follicles and improve the follicle quality.

The invention relates to the technical field of pig feeds, and particularly discloses a special compound feed for weaning sows, the feed comprises a functional additive, the functional additive comprises the following raw materials in parts by weight: 1-3 parts of a plant extract compound, 2-5 parts of conjugated linoleic acid, 0.0003-0.0005 part of selenomethionine, 0.15-0.35 part of vitamin E, and 0.15-0.3 part of vitamin C; the plant extract compound is prepared from the following components: astragalus polysaccharide, icariin, epigallocatechin gallate and a carrier, and the mass ratio of the astragalus polysaccharide to the icariin to the epigallocatechin gallate is 1 to (1 to 4) to (0.2 to 0.8). The special compound feed for the weaned sows is used for feeding the weaned sows through a scientific and reasonable formula, can promote follicular development of the sows after weaning, improve the oestrus rate and reduce the proportion of non-oestrus sows, and can solve the problem of slow ovarian function recovery of the sows in summer high-temperature seasons.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The invention discloses application of paeonol in preparation of a product for improving liver functions and ovarian functions of laying hens in the later egg laying period, belongs to the technical field of feed additives, and particularly relates to application of paeonol in preparation of a product for improving liver functions and ovarian functions of laying hens in the later egg laying period. It is found that paeonol can significantly reduce the activity of glutamic-pyruvic transaminase in the liver, significantly reduce the content of cholesterol and the content of triglyceride in serum, significantly reduce hepatic cell fatty degeneration and lipid droplet aggregation in a hepatic tissue pathological section, relieve liver injury and lipid metabolism disorder, and significantly improve the liver function. The paeonol can effectively improve the fatty liver hemorrhagic syndrome of the laying hens in the later egg laying period, and the paeonol can recover the storage capacity of ovary by improving the survival rate of follicles, improve the egg laying performance of the laying hens and improve the egg laying rate.

The invention discloses a method for promoting ovulation of sexual maturity zebrafish by using Gap27, and relates to the technical field of artificial breeding of fish, a gap connecting protein inhibitor Gap27 is applied to ovulation induction of zebrafish, an intercellular communication network in follicles is accurately intervened, the problem of asynchronous ovulation is solved, and in the final stage of maturation of oocytes, ovulation of sexual maturity zebrafish is promoted. Close substance and information exchange is carried out among follicular granule cells through a gap connecting channel formed by connecting protein Cx43, and Gap27 is used as a specific peptide fragment, can simulate an extracellular ring structure of Cx43, and competitively binds with Cx43 on an adjacent cell membrane, so that formation and functions of the gap connecting channel are physically blocked, and the follicular granule cells are separated from each other. When ovarian tissues of sexually mature female fish are exposed in a Gap27 solution, the transmission of inhibitory signals from granular cells to oocytes can be effectively interrupted, the rupture of nuclear membranes of the cells is promoted, and the final maturation process is completed.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

This invention relates to the field of ovarian tissue in vitro culture technology, and discloses a formulation for activating and increasing follicles and its application. The formulation consists of a PI3K / Akt / mTOR pathway activator and / or a PTEN inhibitor SF1670. The PI3K / Akt / mTOR pathway activator is a 5-15 μM statin, and the PTEN inhibitor is a 20-50 μM SF1670. Using this formulation can achieve the follicle activation and increase effect of existing technologies, contributing to the development and selection of in vitro follicle activators in clinical practice. Furthermore, the use of this formulation opens up a new technological application area for statins.

The utility model provides a follicle flushing liquid constant-temperature device which comprises a constant-temperature box, a box body cover plate is rotationally connected to the constant-temperature box, a fixing opening is fixedly connected to the box body cover plate, the fixing opening is connected with a flushing assembly, an extrusion assembly is arranged in the constant-temperature box, and the extrusion assembly is connected with a constant-temperature assembly. The extrusion assembly achieves the stable and continuous flushing effect and controls the flushing force, the constant-temperature assembly achieves uniform dispersion of heat, so that flushing liquid can be continuously heated, the temperature sensor achieves temperature regulation and control, the constant-temperature state of the flushing liquid is achieved, and the flushing assembly guarantees sterility of medical supplies in contact with the flushing liquid. And control over the flushing flow can be achieved through the arranged flow adjusting valve.

The present application relates to a kind of two-dimensional metal polyphenol complex nanomaterials that can be enriched in ovary, preparation method and application thereof, the nanomaterial can be enriched in ovary, including two-dimensional metal polyphenol complex as substrate, and follicle-stimulating hormone polypeptide grafted to the surface of the substrate;Wherein, the surface of the two-dimensional metal polyphenol complex grafts-COOH functional group and forms 2D MPC-COOH intermediate, the follicle-stimulating hormone polypeptide is covalently grafted with the-COOH on the surface of 2D MPC-COOH intermediate and obtains the two-dimensional metal polyphenol complex nanomaterial.The technical effect of the present application is that the preparation method of nanomaterial is simple, and nanoparticle dispersibility is good, and biosafety is high, and nanomaterial can physically absorb BRD7 target protein through its special structure, block BRD7 into cell nucleus, and then realize the safe down-regulation of KAT7 path and the effective reversal of granulosa cell stress aging.

The application relates to the field of biotechnology, and particularly relates to an application of Bacteroides ovatus in ovarian aging; the application comprises: taking low-abundance Bacteroides ovatus as a biomarker of ovarian aging; the application comprises using Bacteroides ovatus in a medicine for delaying ovarian aging; the application further comprises using a fermentation product of Bacteroides ovatus in the medicine for delaying ovarian aging; it is found through experiments that there is a significant difference in Bacteroides ovatus in mice with different degrees of ovarian aging, and it is found through subsequent experiments that Bacteroides ovatus has the effects of significantly increasing the number of oocytes, up-regulating anti-Mullerian hormone, up-regulating the level of estradiol and down-regulating the level of follicle-stimulating hormone, which indicates that Bacteroides ovatus can be used in the medicine for delaying ovarian aging.

Disclosed are a method and culture medium for performing an in vivo treatment on ovaries of mammals including humans or performing in vitro culturing on ex vivo ovaries or ovarian tissue by means of using 4-vinylcyclohexene diepoxide. The method or culture medium provided facilitates the retention and enhancement of the developmental and reproductive potential of mammalian ovaries or ovarian tissue, and is particularly capable of promoting follicle maturation and increasing the number of ovulations.

The application discloses a superovulation method based on synergistic effect of composite hormones, which comprises the following steps: injecting PMSG and FSH to induce estrus, and injecting hCG to promote synchronous ovulation. The application adopts synergistic regulation of PMSG (long-acting follicle-stimulating hormone) and FSH (short-acting follicle-stimulating hormone), PMSG completes follicle recruitment, and FSH finely regulates the development stage, thereby avoiding overgrowth of follicles. In the examples, the Burmese cat, the British short cat and the farm cat do not appear OHSS symptoms (such as ovarian enlargement and ascites), and the ovarian state is normal.

The invention discloses a live sow egg collecting instrument system, and belongs to the technical field of livestock breeding detection. A living sow egg collecting instrument system comprises a host, an ultrasonic probe, a negative pressure pump, an egg collecting tube, an egg collecting needle and an operation system. The host is a control center of the system, integrates an ultrasonic signal processing module, a negative pressure adjusting module and a data recording module, and is used for receiving signals transmitted by the ultrasonic probe, adjusting the pressure of the negative pressure pump and recording key data in the egg collecting process. The ultrasonic probe is electrically connected with the host, is used for transmitting and receiving ultrasonic signals and is inserted into the dome part of the vagina of the sow, and after the operation system is started, the probe is in contact with ultrasonic images of ovaries and follicles of the sow and displays the ultrasonic images on a host screen; the problems that in the prior art, the utilization rate of improved variety resources is low, the egg collecting accuracy and efficiency are insufficient, the egg quality is not guaranteed, and data management is disordered cannot be effectively solved.

A sensor comprising a whisker shaft and a follicle is provided. The shaft has a root end and a tip end and the shaft tapers from the root end to the tip end so that the root end is wider and the tip end is narrower. The root end is pivotably mounted in the follicle.

This invention discloses a Polygonatum sibiricum vesicle preparation for treating premature ovarian failure, its preparation method, and its application. The preparation method includes: cleaning Polygonatum sibiricum, juicing, filtering to remove residue, and collecting the juice; subjecting the juice to gradient centrifugation to remove residual plant tissue, plant cells, and plant cell debris; then using an extraction reagent for stepwise precipitation, collecting the precipitate, and resuspending it to obtain the final product. The Polygonatum sibiricum vesicle preparation prepared by this invention can be efficiently taken up by ovarian granulosa cells. It effectively treats premature ovarian failure by activating the Nrf2 signaling pathway, upregulating antioxidant enzyme expression, scavenging reactive oxygen species, restoring mitochondrial membrane potential, inhibiting apoptosis, and restoring the expression levels of estrogen, follicle-stimulating hormone, and anti-Müllerian hormone. The preparation method of this invention is standardized, biocompatible, and safe, and has broad clinical application prospects.

The application discloses a micro-needle artistic hair transplantation follicle intelligent precise positioning system and relates to the technical field of hair transplantation follicle image processing. The system determines a hair transplantation area through obtained three-dimensional network imaging of follicles, establishes a two-dimensional coordinate system taking the target area to be measured as a reference to preliminarily plan the planting density, further divides the follicle density gradient according to the determined hair transplantation area, marks the coordinate positions of the follicles, forms a visual follicle planting point distribution map, provides reference points for the positioning of the micro-needle, and finally monitors the skin deformation and the deviation of the micro-needle positioning coordinates in the spatial feature coordinate system in real time, adjusts the micro-needle puncture positioning, realizes the precise positioning alignment of the micro-needle and the target follicle, and solves the problem of low accuracy of the intelligent positioning of the hair transplantation follicle in the process of converting the position image coordinates of the hair transplantation area to the mechanical arm coordinate system when the micro-needle carries out the planting operation according to the feedback information.

The invention relates to the technical field of biological breeding and artificial intelligence image recognition, in particular to a chicken follicle ultrasonic image automatic detection model construction method and system based on deep learning. Aiming at the problems of unclear follicle boundary identification, easy missing detection of small-size follicles, easy identification of adjacent follicles as a single target in an ultrasonic image and the like when a conventional target detection model is adopted to process a chicken follicle ultrasonic image under the condition that targeted structure optimization is not performed in the prior art, the model is improved, and the follicle boundary identification accuracy is improved. Therefore, the improved model can more accurately identify the follicle center echo region and the edge structure thereof, the adjacent follicles can be respectively labeled, and the positioning result of the follicles in the ultrasonic image is clearer and more stable.

The invention relates to the technical field of ovarian ultrasound image enhancement, in particular to a polyovarian syndrome auxiliary diagnosis method and system based on image recognition. The method comprises the following steps: segmenting an ovary ultrasound image of a patient, analyzing the pixel gray level and distance distribution of an ovary region, evaluating the image recognition degree, clustering, and recognizing a potential follicle region. Any region is selected as a reference region, and the sinus degree is calculated according to the boundary gray level change and the internal gradient of the region; evaluating the follicle arrangement degree in combination with the position distribution and the neighbor distance; and calculating the change degree of the multi-capsule sample through the area proportion. And integrating the three indexes of follicle areas of all patients to mark to-be-enhanced levels, thereby enhancing the image and assisting in diagnosis of the polycystic ovarian syndrome. According to the method, targeted image enhancement can be carried out on all potential follicle regions, the omission ratio is reduced, and thus a relatively accurate diagnosis result is obtained.

The invention provides a breeding method for improving the breeding rate of replacement gilts, and the breeding method comprises the following steps: (1) adding a first functional nutritional composition into feed from 130-150 days old to the early estrus of the replacement gilts; (2) adding a second functional nutrient composition into the feed from the initial estrus period of the replacement gilts to the time before mating; and (3) within 25-35 days after mating of the replacement gilts, adding a second functional nutrient composition into the feed. According to the breeding method, oxidative stress of the replacement gilts can be relieved, development and maturation of follicles can be adjusted, oestrus and ovulation of the gilts can be promoted, the initial estrus period of the replacement gilts can be started in time, embryonic development can be promoted, the conception rate of the replacement gilts can be increased, and the replacement gilts are prevented from being eliminated due to delayed oestrus or non-oestrus or repeated breeding and infertility after oestrus; further, the breeding rate of the replacement gilts is improved.

The invention belongs to the cross technical field of regenerative medicine and traditional Chinese medicine, and discloses a composition for treating premature ovarian failure and a preparation method and application thereof. The composition comprises adult stem cells, traditional Chinese medicine active components consisting of astragaloside and salvianolic acid B in a molar ratio of (1: 1)-(4: 1) and a biodegradable slow-release carrier, and double intervention of'cell repair-microenvironment regulation and control 'is realized through a synergistic strategy of'pretreatment of stem cells by traditional Chinese medicines and targeted slow-release delivery'. Experiments prove that the composition can enable the estrus cycle recovery rate of premature ovarian failure model animals to reach 70%-90% and the number of follicles to be increased by 6-8 times, is obviously superior to the existing single therapy, has a clear mechanism (activating a PI3K / Akt pathway and inhibiting a TGF-beta1 / Smad pathway), and has outstanding clinical transformation value.

The invention discloses a bionic delivery system and an anhydrous composition for enhancing hair follicle penetration. The system comprises a mesoporous nanocarbon core, a pH responsive charge reversal polymer layer, a bionic interface lipid regulation layer and at least two loaded oligopeptide precursor molecules. The polymer layer is protonized and positively charged in a scalp microenvironment, so that a hair follicle opening is accurately anchored; the lipid regulation layer temporarily enhances the lipid fluidity of hair follicles in a non-invasive manner and promotes the permeation of active matters; the oligopeptide precursor is triggered by alkaline phosphatase in the deep part of the hair follicle and is self-assembled into the active target oligopeptide through in-situ biological orthogonal connection. A composition comprises the nanosystem, a natural deep eutectic solvent, a plant source emollient and a zinc salt complex, and synergistically enhances permeation and release. The system realizes accurate, efficient and safe delivery of active matters in the deep part of hair follicles, and is suitable for prevention and treatment of hair follicle related diseases.

The invention relates to the field of efficient breeding of animals, in particular to a sheep estrus synchronization kit based on follicular wave induction and turnover, application and a use method. The kit contains estradiol benzoate, vitamin ADE, a GnRH analogue buserelin, a progesterone vaginal sustained-release agent, pregnant mare serum gonadotropin and a prostaglandin F2alpha analogue cloprostol sodium according to time nodes, and is matched with instructions for use. According to the method, follicle waves are induced or reset through estrogen and vitamin ADE on the 0th day, buserelin is injected on the 5th day, a progesterone sustained-release agent is placed, follicle development is synchronized, thrombus is removed on the 12th day, PMSG and cloprostenol sodium are jointly injected, follicle final maturation and ovulation are triggered, and estrus testing and mating are conducted 12-96 h after thrombus is removed. According to the scheme, high oestrus rate and highly concentrated oestrus time windows can be obtained for milk goats in different physiological states in the breeding season and the non-breeding season, regular artificial insemination can be conveniently implemented, and the breeding efficiency of a large-scale sheep farm is improved.

The invention belongs to the technical field of biological medicine manufacturing, and particularly relates to a pharmaceutical preparation for improving ovarian functions and a preparation method thereof. The pharmaceutical preparation comprises the following raw materials in parts by weight: 12-25 parts of berberine hydrochloride, 8-16 parts of shionone, 5-10 parts of dicliptera chinensis polysaccharide, 10-20 parts of a diluent, 5-9 parts of an adhesive and 1-6 parts of a lubricant. According to the pharmaceutical composition, berberine hydrochloride, shionone and dicliptera chinensis polysaccharide are compounded, sexual cycle disorder caused by the polycystic ovarian syndrome is adjusted in a synergistic interaction mode, insulin resistance is improved, indexes such as luteinizing hormone (LH), follicle-stimulating hormone (FSH) and LH / FSH are remarkably improved, and the pharmaceutical composition has the application potential of treating the polycystic ovarian syndrome and has good application prospects. And the market prospect is wide.

The invention provides an application of an E3 ubiquitin ligase RLIM gene inhibitor in preparation of a medicine for treating ovarian aging or injury. Experiments find that the expression level of the RLIM gene in the ovary is positively correlated with the aging degree, and the expression of the RLIM gene is remarkably improved along with age increase. In-vivo and in-vitro experiments show that ovarian granular cell senescence can be effectively delayed and ovarian functions can be improved by inhibiting RLIM expression, and particularly, the proportion of atresia follicles is reduced, the number of healthy follicles is increased, and serum AMH and E2 levels are remarkably improved. The pharmaceutical composition provided by the invention comprises a siRNA inhibitor (preferably, siRLIM-2) of specific targeting RLIM and a nano-liposome carrier, and efficient targeting delivery can be realized through tail vein injection. The treatment scheme has no toxic effect on important organs, is high in safety, and provides a new effective means for treatment of ovarian aging and related diseases.

Methods for producing an artificial follicle are disclosed. Also disclosed are artificial follicles produced by such methods, in vitro methods of rejuvenating an oocyte, and methods of treating infertility or improving fertility.

The application discloses a kind of multi-factor risk assessment methods for ovarian hyperstimulation, it is related to intelligent medical technical field, including, based on multi-source baseline data package, the trend of follicle volume change with time is calculated by ovarian response simulation, obtain follicle response curve, the probability of vascular injury is calculated by puncture path hemorrhage simulation, obtain bleeding probability, and the follicle response curve and bleeding probability are fused, form patient characteristic risk vector;According to the intervention suggestion in the structured strategy list is associated with ovarian hyperstimulation risk score and bleeding risk score to generate risk change description, and form risk assessment report according to risk change description;The application constructs the patient characteristic risk vector by fusing follicle dynamic response and puncture bleeding risk, effectively enhance the safety and individualization level of assisted reproductive treatment.

The invention discloses a special veterinary B-ultrasonic system for pig follicle AI intelligent detection, and relates to the technical field of veterinary medical instruments. The device comprises a handle seat, the tail end of the handle seat is provided with a connecting wire seat, the front end of the handle seat is provided with an adjustable detection assembly, and the adjustable detection assembly comprises a connector. According to the invention, through a thread transmission mechanism formed by the adjusting screw rod, the threaded pipe and the limiting plate, manual rotation operation is converted into linear telescopic motion of the detection head assembly, and continuous, stable and adjustable working length of the probe is realized. The mechanical structure overcomes the defects that a universal probe in the prior art is generally insufficient in length and cannot adapt to the bending of the genital tract of a pig, so that the probe can be accurately adjusted to the optimal detection depth according to the body type and anatomical structure difference of different pigs and can go deep along a physiological bending track, thereby eliminating a detection blind area and improving the detection accuracy. The follicle image acquisition rate and definition are improved, and the risk of operation injury is reduced.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.