116 results about "Follicle" patented technology

The invention relates to the field of hypothermal medicine of biotechnology, in particular to a cryoprotectant free of DMSO (dimethyl sulfoxide). The cryoprotectant comprises 200 mmol/L of trehalose, 0.3 g/mL of PVP (polyvinylpyrrolidone) 40, 200 mu g/L of cholesterol and 200 mu g/L of lecithin. The cryoprotectant is injected into a newly obtained ovary; all macroscopic follicles on the surface of the ovary are punctured and sucked, so that content in the follicles is removed; then the ovary is put in a freezing bag to be cooled. The cryoprotectant doesn't contain DMSO, does not have cytotoxicity and is easy to remove, simple to prepare and good in cryoprotective effect, raw materials are easy to obtained, the rate of normal primordial follicles is high, and the number of dead cells is small; an ovary cryopreservation method is simple and easy to operate and good in cryoprotective effect; the histological structure can be better conserved after the whole ovary of sheep is frozen.

The invention provides recombinant forms of piscine follicle-stimulating hormone (FSH) with characteristic intramolecular disulfide bonds and modified glycosylation patterns in the β-subunit that enhance the stability and metabolic activity of the hormone. Also provided are recombinant materials to produce the FSH β and glycoprotein α-subunits singly or in combination to obtain complete heterodimeric hormone of regulated glycosylation pattern. The piscine FSH agonists of the invention are therapeutically useful to expedite the onset of puberty in captive fish and to alleviate reproductive dysfunctions in fish. Likewise, the piscine FSH antagonists of the invention will be therapeutically useful to halt gonadal development, thereby contributing to body weight gain of the fish,

The invention belongs to the technical field of traditional Chinese medicines and in particular relates to a traditional Chinese medicine composition for treating female infertility and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-10 parts of epimediums, 10-15 parts of semen cuscutae, 10-60 parts of astragalus membranaceus, 5-15 parts of human placenta, 10-15 parts of white atractylodes rhizome, 5-15 parts of angelica, 6-12 parts of ossa sepiae, 5-10 parts of pulp of dogwood fruit, 10-15 parts of fructus lycii, 3-10 parts of scutellaria baicalensis, 6-12 parts of rhizoma cyperi and 3-10 parts of ligusticum wallichii. The traditional Chinese medicine composition has the effects of warming yang, benefiting vital energy, nourishing blood, replenishing essence, regulating vital energy and removing blood stasis; the raw materials are carefully selected, metaplasia of follicle and restoration of reproductive function and organs such as the kidney, the heart, the liver and the spleen are promoted, and a remarkable treatment effect on the female infertility is achieved.

The invention discloses a long-acting recombinant human follicle-stimulating hormone-Fc fusion protein (referred to as hFSH-Fc) and a preparation method thereof. The hFSH-Fc protein is dimerization fusion protein, the amino acid sequence of the protein sequentially comprises an hFSH beta subunit, CTP, a hFSH alpha subunit, a flexible peptide joint and a human IgG Fc variant from the N end to the C end, and the in vivo half-life period of the protein is longer than that of the existing human follicle-stimulating hormone, so that the efficacy is better. The invention also relates to a use of a recombinant hFSH-Fc fusion protein composition in preparation of drugs in the animal breeding field.

The invention relates to nutritional supplement for milking sows as well as a preparation method and application thereof. The nutritional supplement for the milking sows comprises the following components in parts by weight: 50 to 80 parts of expanded full-fat soybean, 10 to 30 parts of functional microcapsule coated fat powder and 1 to 10 parts of a calcium supplement. The raw materials are screened and optimized, so that the intake on nutritional substances such as protein and fat by sows in the suckling period (in particular in summer, the feed intake of the sows is low and the lactation demand of the sows cannot be met) is increased, the milk performance of the sows is improved, the follicle quality is improved and oestrus is promoted.

The invention belongs to the field of Chinese medicine pharmacy, and relates to a traditional Chinese medicine composition for treating menopausal syndrome and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises traditional Chinese medicine radix rehmanniae, radix paeoniae alba, rhizoma anemarrhenae, golden cypress, morinda officinalis, herba epimedii, raw oyster shell, crude dragon bone, radix bupleuri and tuber fleeceflower stem as main medicines, and is blended with auxiliary materials to be prepared into the preparation for treating the symptoms related to menopause, i.e. the heating, the sweating, the agitated emotion, the insomnia and the like. The experiment proves that the traditional Chinese medicine composition provided by the invention has the definite functions for improving the blood serum E2 and reducing the blood serum FSH (follicle-stimulating hormone), the hypothalamus estrogen receptors Er alpha, Er beta gene expression and protein expression can be continuously adjusted, and the situation of hypothalamus aging can be improved, so that the menopausal syndrome can be effectively cured.

The invention provides a composition and feed for feeding coccinella septempunctata, and a preparation method of the composition. The composition comprises the following components in parts by weight: 500-1000 parts of aphid treatment substances, 1-4 parts of [beta] carotene, 0.000001-0.00001 part of juvenile hormone and the like. The feed composition provided by the invention comprises the aphid treatment substance, the juvenile hormone and the [beta] carotene, wherein the aphid treatment substance has relatively comprehensive basic nutrients, the juvenile hormone has the characteristic of promoting ovarian development of adults, and the [beta] carotene has the characteristics of improving the survival rate of insects and protecting follicles and uterine cells. The egg laying amount and the hatching rate of the coccinella septempunctata fed by the composition have no significant difference from the egg laying amount and the hatching rate of the coccinella septempunctata fed by conventional bean aphids, and the feed is favorable to be stored and is conveniently used. The whole demand quantity of aphids can be reduced by about 20%, the space is saved by 50% or above, and the labor demand is reduced by 30% or above.

The invention discloses a traditional Chinese medicine composition for treating diminished ovarian reserve combined infertility as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 10-20 parts of radix angelicae sinensis, 10-20 parts of radix paeoniae alba, 10-20 parts of dried radix rehmanniae, 10-20 parts of fructus corni, 10-20 parts of semen cuscutae, 10-20 parts of human placenta, 10-20 parts of fructus ligustri lucidi, 10-20 parts of herba ecliptae, 10-20 parts of herba epimedii, 10-20 parts of rhizoma dioscoreae, 10-20 parts of semen euryales, 10-20 parts of radix codonopsis, 10-20 parts of himalayan teasel root and 3-10 parts of baked licorice. Tests prove that, according to the traditional Chinese medicine composition for treating the diminished ovarian reserve combined infertility, the periodic ovulation rate, the single follicle ovulation rate, the follicle output rate, the intra-day intima thickness of HCG injection and the periodic pregnancy rate are increased, and the traditional Chinese medicine composition has a good treatment effect on patients suffering from the diminished ovarian reserve combined infertility.

The invention discloses a separation, primary culture and subculture method for granulosa cells in follicles in a pig ovary GV period. The method comprises the following steps: collecting a cyst-free healthy pig ovary, and cleaning the ovary; separating granular cells; carrying out primary culture on the granular cells for 48 hours; changing liquid in a culture bottle for primary culture; after changing the liquid, continuously culturing the cells for 36 hours, and cleaning, digesting and terminating the cultured cells; and finally, sub-packaging and subculturing the cells for 48 hours. The invention provides a pollution-free ovary collection method, optimizes a granulosa cell acquisition and culture method, and further provides a primary granulosa cell subculture method. Granular cells obtained by the method are almost free of pollution, high in cell uniformity, high in consistency in the growth and development period, high in cell survival rate, high in success rate of making oxidative stress models and the like, and high in result consistency, practicability, stability and feasibility. In addition, the number of obtained cells is large, many subsequent experiments can be carried out, the number of times of going to a slaughter house is reduced, and the efficiency is high.

The invention relates to a composition for treating acne, a preparation method therefor and application of the composition. The composition for treating the acne, prepared by the preparation method, has the effects of inhibiting secretion of sebum, improving keratinization of follicle sebaceous gland catheters, inhibiting pathogenic microorganisms such as propionibacterium acnes, alleviating inflammatory reactions, alleviating pigmentation, preserving moisture and repairing skin barrier and is comprehensive in action and relatively good in treatment effect. The composition for treating the acne, disclosed by the invention, can be used for preserving moisture, alleviating pigmentation and repairing skin barrier while removing acne, so that fat is controlled, and skin is comfortable and notdry. The composition for treating the acne, disclosed by the invention, is simple in prescription and production process, raw materials employed all are in line with national specifications for cosmetic raw materials, the use safety is good, and the composition has a broad market prospect.

The invention relates to a method for improving low ovarian response in patients with polycystic ovary syndrome by transvaginal small follicle puncture. The method comprises case inclusion, measurement indicators, and standard operating procedures; The case inclusion comprises inclusion criteria and exclusion criteria; The measurement indicators comprise main research indicators, follicular fluidanalysis time, secondary research indicators, serum and follicular fluid analysis time, and safety indicators; The standard operating procedures comprises a clinical ovulation promotion protocol and aTVOD method. The advantages of the method of the invention are as follows: the method of COS for improving the low ovarian response in patients with PCOS can be performed on the day after the IVF ovulation promotion cycle immediately, and the effect of COS for improving the low ovarian response in patients with PCOS on the day after the IVF treatment cycle can be evaluated, and the underlying mechanisms can be studied.

The invention relates to a system for calculating the age limit of new change of ovarian reserve of a subject, and the system comprises a data acquisition module which is used for obtaining the age, anti-mullerian hormone level, follicle-stimulating hormone level and sinus follicle counting data of the subject; a module for calculating an ovarian reserve function which uses a subject working characteristic curve for detecting the demarcation points of the age, the anti-mullerian hormone level, the follicle-stimulating hormone level and the sinus follicle counting of the subject acquired in thedata acquisition module, converts the age, the anti-mullerian hormone level, the follicle-stimulating hormone level and the sinus follicle count into dichotomy variables according to the tangent point value of the demarcation point and calculates the current low ovarian response probability (p0) of the subject by using the dichotomy variables as prediction variables; and a module for calculatingthe age limit of the subject when the ovarian reserve of the subject is reduced to a certain degree, which is used for calculating the age limit of the subject when the ovarian reserve of the subjectis newly changed by utilizing the current ovarian reserve, namely the ovarian low-response probability (p0).

The embodiment of the invention discloses a method suitable for manufacturing ovarian paraffin sections of equus animals, and relates to the technical field of biological research. The method comprises the following steps: sampling and fixing, dehydrating, transparentizing, waxing, exhausting, embedding and section manufacturing. According to the method suitable for manufacturing the ovary paraffin sections of the equus animals, the air exhaust process is added; bubbles existing in ovarian tissues of equus animals in the waxing process are effectively removed; the ovary paraffin section of equus animals prepared by the method can effectively protect the integrity of the tissue structure of the ovary of equus animals, and can obtain a good staining effect, and the integrity of follicles canbe maintained to a great extent, so that good paraffin dipping and embedding effects are obtained.

The invention relates to a puncture ovum taking device capable of conveniently detecting the follicle form. The puncture ovum taking device comprises a puncture needle, a flushing device, a negative pressure device, a test tube, a puncture needle frame and a handle part. A flushing hose is connected to the flushing device, and the test tube and the negative pressure device are connected through ahose and connected with an ovum conveying connecting tube; and a main channel in the handle part, an axis cavity in the puncture needle frame and the cavity channel of the puncture needle sequentiallycommunicate with one another. An ultrasonic probe is arranged on the outer wall of the end, connected with the puncture needle, of the puncture needle frame, and a data line connected with the ultrasonic probe extends out of the handle part and can be connected with external equipment, or the ultrasonic probe is connected with a wireless communication module so that the ultrasonic probe can be connected with external equipment in a wireless transmission mode. By means of the puncture ovum taking device, relatively clear follicle distribution images can be obtained so as to provide a good observation basis for puncture ovum taking operation, operation can be conducted smoothly and conveniently, and the ovum with good quality can be sucked.

The invention discloses a chicken small yellow follicle outer membrane layer cell separation and culture method. The method comprises the following steps: collecting small yellow follicles; acquiring outer membrane layers of the small yellow follicles; cleaning, shearing, digesting and filtering the outer membrane layers; performing primary culture on outer membrane layer cells for 36 to 48 hours; after changing liquid, continuing to perform culturing for 36-48 hours; and finally, performing subculture on the cultured cells for 24-48 hours. The invention provides a pollution-free method for collecting the small yellow follicles, optimizes a method for obtaining outer membrane layers of the small yellow follicles and culturing membrane cells, and further provides a method for subculturing primary membrane cells. By using the method provided by the invention, the membrane cells with relatively high purity can be separated, the consistency in the growth and development period is high, the cell survival rate is high, the success rate of a cell proliferation detection test is high, the result consistency is high, the practicability is strong, the stability is strong, and the feasibility is high. The small yellow follicles collected by the method provided by the invention have a large quantity of cells, and can be continuously supplied to subsequent experiments, so that the poultry purchase cost is reduced, and the experiment efficiency is improved.

The invention provides a traditional Chinese medicine composition for treating or improving polycystic ovarian syndrome as well as a preparation method and application thereof The traditional Chinese medicine composition is prepared from the following raw materials of semen cuscutae, placenta hominis, poria cocos, radix puerariae, folium photiniae, radix salviae miltiorrhizae, rhizoma atractylodis fried with bran, prepared radix morindae officinalis, radix rehmanniae praeparata, roasted herba epimedii, spina gleditsiae, cornu cervi, radix gentianae, fried semen cassiae, cleaned fructus crataegi, rhizoma alismatis, folium nelumbinis and salted semen plantaginis. According to the traditional Chinese medicine composition as well as the preparation method and the application thereof provided by the invention, the reproductive disorder and metabolic disorder of the polycystic ovarian syndrome can be improved at the same time, and the SCFA level in intestinal tracts can be obviously improved; the levels of proinflammatory factors such as serum TNF-alpha, IL-1beta and IL-6 are reduced, so that the chronic hyporeactive inflammation is improved; the level of adiponectin receptors in fat, skeletal muscle and ovarian tissue is improved, and finally the insulin resistance of the polycystic ovarian syndrome is obviously improved; and the ovarian function of the polycystic ovarian syndrome and the maturation and development of follicles can be improved.

The invention discloses a composition for improving ovarian function. The composition comprises umbilical cord mesenchymal stem cells and umbilical cord blood CGF. The functions of stem cells and CGFare maximized through an ovary multi-site injection administration mode, so that the ovary weight, the follicle number of each stage and the E2 level of an ovarian premature senility model rat are increased, the FSH level and the ovarian granulocyte apoptosis rate are reduced, the Bax protein is remarkably reduced, and the Bcl2 protein is increased. The invention provides a powerful tool for clinically treating the premature ovarian failure and improving the ovarian function.

The invention relates to the technical field of isolated culture of ovarian granulosa cells, in particular to an isolated culture method of duck ovarian granulosa cells. The isolated culture method comprises the following steps: (1) bleeding necks of female ducks to kill the female ducks, and taking out complete ovaries; (2) shearing complete follicles before ovulation to obtain single follicles before ovulation; (3) cutting off the single washed follicles before ovulation, scraping off yolk, and washing for 5 times; (4) respectively digesting cleaned granulosa cell layers by adopting hyaluronidase and trypsin; (5) resuspending the digested granulosa cell layers, conveying an obtained cell suspension into a culture medium, and repeatedly blowing and beating by using a pipettor to dispersecells; and (6) carrying out incubator culture on the resuspended cell suspension. The duck ovarian granulosa cells obtained by the isolated culture method provided by the invention are high in purityand high in activity, and the survival rate of the duck ovarian granulosa cells is high.

The invention discloses an application of pyrroloquinoline quinone (PQQ) in prevention and/or treatment of female reproductive dysfunction. Research results of the invention show that supplement of pyrroloquinoline quinone can significantly improve follicle quantity and quality and reduce fertility of model mice. According to the invention, pyrroloquinoline quinone is used for treating reproductive function reduction caused by quantity reduction or quality reduction of follicles for the first time, a relatively ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of female reproductive dysfunction, and the pyrroloquinoline quinone has extremely high application value.

The invention belongs to the technical field of traditional Chinese medicine, and particularly discloses a medicine for treating infertility and a preparation method thereof. The medicine is prepared from fried semen cuscutae, cornu cervi degelatinatum, roasted morinda officinalis, fried eucommia ulmoides, fried teasel roots, fried Chinese yam, fried gorgon fruits, fructus lycii, amomum villosum lour, fried rhizoma atractylodis macrocephalae, codonopsis pilosula, prepared rehmannia roots, angelica sinensis, roasted astragalus membranaceus and roasted liquorice. The medicine for treating infertility is beneficial for increasing the thickness of the endometrium, enhancing the follicle tension, recovering normal development of follicles and enhancing pregnancy assistance.

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine composition for treating infertility and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 25-30 parts of radix rehmanniae praeparata, 12-15 parts of radix angelicae sinensis, 25-35 parts of fried rhizoma atractylodis macrocephalae, 15-20 parts of fried rhizoma dioscoreae, 10-12 parts of fried radix paeoniae alba, 9-12 parts of raw semen ziziphi spinosae, 6-10 parts of cortex moutan, 9-12 parts of radix glehniae, 8-10 parts of fried cortex eucommiae, 20-25 parts of radix codonopsis, 6-10 parts of radix bupleuri, 8-12 parts of fried rhizoma cyperi and 6-9 parts of caulis perllae. The traditional Chinese medicine composition for treating infertility, provided by the invention, can improve the curative effects of increasing the thickness of endometrium, enhancing follicle tension and recovering normal development of follicles, and finally effectively assists pregnancy.

The invention discloses an ovary and/or follicle automatic measurement method based on a two-dimensional image. The ovary and/or follicle automatic measurement method includes the steps that a two-dimensional ultrasound image to be measured is acquired; and object parameters of a target object carried by the two-dimensional ultrasound image to be measured are automatically measured to obtain measurement information corresponding to the two-dimensional ultrasound image to be measured, so that automatic measurement of the target object is realized. By automatically identifying the object parameters of the target object in the two-dimensional image and outputting monitoring results according to the object parameters, the operation steps of a doctor are reduced, errors introduced by manual marking are reduced, consistency of measurement results is ensured, and the diagnosis efficiency is improved.

The invention discloses an additive for improving ovarian development and later egg laying performance of Taihe black-bone chickens as well as a preparation method and application of the additive, and belongs to the technical field of feed additives. The additive contains fermented traditional Chinese medicines, organic calcium, polyphenol substances, vitamin E, beneficial bacteria and a carrier, the fermented traditional Chinese medicines comprise fermented pomegranate rind, fermented purslane, fermented poria cocos, fermented wild fungus flowers, fermented motherwort, fermented angelica sinensis and fermented honeysuckle; the organic calcium comprises calcium citrate and calcium formate; the polyphenol substance comprises mulberry leaf polyphenol; the beneficial bacteria comprise brevibacillus laterosporus, lactobacillus cellobiosus, enterococcus faecium and bifidobacterium bifidum; the carrier comprises corn flour. The additive can promote the laying rate of the Taihe black-bone chickens, improve the egg weight, reduce the feed-egg ratio, reduce the egg breaking rate and improve the egg shell quality; meanwhile, the number and relative weight of dominant follicles can be increased, the number and relative weight of small follicles can be reduced, ovarian development of the Taihe black-bone chickens is improved, and the problem that the egg laying performance and the egg quality are reduced in the later egg laying period is solved.

The invention discloses a pharmaceutical composition for warming kidney and tonifying yang. The pharmaceutical composition is prepared from the following raw material medicines in parts by mass of 7-13 parts of herba epimedii, 7-13 parts of rhizoma drynariae and 7-13 parts of herba cistanche. The kidney warming and yang tonifying compound can improve the uterus microenvironment from the aspects of serum sex hormone, endometrial thickness and gland function, female progestational hormone receptor expression, uterus microvessel density, uterus local area factors and the like, can also improve the ovum function, enhance ovarian reserve, promote oocyte maturation and inhibit cell excessive apoptosis, the occurrence of abnormal ova and atresia follicles is reduced, the positive improvement effect on Chong and Ren deficiency reproductive disorder is achieved, and the pregnancy outcome is changed.

The invention relates to a technical system for programmed cryopreservation of mammal ovarian tissue, and belongs to the technical field of tissue cryopreservation. The ovarian tissue frozen and resuscitated by the method can keep good physiological activity. Ovarian tissue freezing not only can preserve the function of ovarian generation gametes, but also can keep the endocrine function of the ovarian generation gametes, and has incomparable advantages in fertility preservation. After the ovarian tissue is obtained and treated, the ovarian tissue is balanced by a cryoprotectant and cooled bya programmed freezing instrument, and the ovarian tissue is stored in liquid nitrogen. By improving the components and proportion of the cryoprotectant, the balance time and the freezing and cooling procedures, follicles in ovarian tissues can be well protected from being damaged by freezing resuscitation stimulation, and a feasible method is provided for protecting and storing the fertility of mammals.

The invention provides a traditional Chinese medicine Li medicine composition for reducing an ovarian reserve function. The traditional Chinese medicine Li medicine composition is prepared from 18-22 parts of radix rehmanniae preparata, 13-17 parts of entada phaseoloides, 13-17 parts of radix angelicae sinensis, 13-17 parts of radix paeoniae alba, 18-22 parts of rhizoma polygonati, 10-14 parts of fructus corni, 18-22 parts of radix puerariae, 18-22 parts of caulis spatholobi, 18-22 parts of semen cuscutae, 8-12 parts of Jianqu, 4-6 parts of agilawood and 2-4 parts of radix glycyrrhizae. The invention further provides a corresponding traditional Chinese medicine Li medicine preparation and application thereof. According to the invention, the traditional Chinese medicine Li medicine composition can effectively increase the blood supply of ovary, improve the local microenvironment of the ovary and the uterus, and further increase the number of sinus follicles by adjusting the hypothalamus-pituitary-ovary reproductive axis, increasing the reaction of the ovary to gonadotropin and adjusting internal secretion, so that the clinical treatment effect is improved, the clinical symptoms of patients are improved, and the outcome of DOR and infertility is improved; and the traditional Chinese medicine Li medicine composition has low toxic and side effects, low price, local characteristics and wide market prospect and development value.