The invention relates to the technical field of
image processing, and discloses a fluorescent lifetime
microscopic image large-view-field splicing method and
system and
electronic equipment, and the method comprises the steps: constructing a tissue region
mask for a to-be-spliced image, and constructing a
vignetting model in a tissue region to complete
vignetting correction; counting brightness indexes in an organization area to determine a global brightness reference, and realizing image group brightness unification through global zooming and
single image brightness adaptive correction; further realizing alignment of adjacent images through
feature point matching, and finishing image block splicing in combination with a minimum
color difference suture fusion method to obtain an image band; an overlapping area is extracted from an image belt to generate an effective content
mask,
relative displacement is estimated by using a
phase correlation method after low-frequency suppression, image belt splicing is completed by
multiplexing a minimum
color difference suture fusion method, a large-view-field spliced image is output, the
automation degree and robustness of splicing are improved, the spliced image is geometrically consistent and visually seamless, and the splicing efficiency is improved. And the requirements of
medical research on high-resolution and large-field-of-view
fluorescence lifetime microscopic images are met.