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The present invention provides a plate (1; 2) comprising an array of pores (11; 21) for carrying out chemical or biological reactions, wherein each of the pores (11; 21) comprises a reaction chamber having at least one opening (111; 211) on a surface (12; 22) of the plate (1; 2). The array of pores (11; 21) is composed of a plurality of adjacent pore blocks (13; 23), wherein each pore block (13; 23) is composed of a plurality of adjacent rows of pores, and wherein at least one blank portion (14; 24) is provided between the rows of pores in each pore block (13; 23). Here, the blank portion (14; 24) is specifically a portion of the surface (12; 22) of the plate (1; 2) and has no pore opening (111; 211). Furthermore, the present invention provides a method for multiple imaging of such a plate (1; 2) using an imaging system.

This application presents a gRNA probe library, its preparation and design methods, a method for targeting genes, and a nucleic acid imaging method. This application combines gRNA probes containing modified groups with nucleases, enabling dynamic observation of DNA and RNA by binding to target genomic sequences or RNA transcripts in living cells. By utilizing fluorescently labeled crRNAs, tracrRNAs, and sgRNAs, this application achieves real-time imaging of repetitive and non-repetitive sites, establishing a powerful gene imaging toolkit. Furthermore, the gRNA probe library prepared in this application will be applied to CRISPR systems for gene editing, transcriptional regulation, gene targeting, and delivery.