225 results about "Polyglutamate" patented technology

The invention discloses a chewable composition for oral delivery of bioactive agents having nanoparticles that are composed of chitosan, poly-glutamic acid, and at least one protein drug or bioactive agent characterized with a positive surface charge and their enhanced permeability for paracellular protein drug and bioactive agent delivery.

Disclosed are water soluble compositions of paclitaxel and docetaxel formed by conjugating the paclitaxel or docetaxel to a water soluble polymer such as poly-glutamic acid, poly-aspartic acid or poly-lysine. Also disclosed are methods of using the compositions for treatment of tumors, auto-immune disorders such as rheumatoid arthritis. Other embodiments include the coating of implantable stents for prevention of restenosis.

A nucleic acid formulation for use in gene delivery comprising a nucleic acid and an anionic polymer is disclosed. Examples of the anionic polymer includes anionic amino acid polymer or poly-amino acid (such as poly-L-glutamic acid, poly-D-glutamic acid, poly-L-aspartic acid, poly-D-aspartic acid), poly-acrylic acid, polynucleotides, poly galacturonic acid, and poly vinyl sulfate.

Various biodegradable polyglutamate conjugates comprising recurring units of the general formulae (I), (II), (III), (IV), (V), and / or (VI) are prepared. The polymers are conjugated with a plurality of drugs. Such polymer conjugates are useful for variety of drug, targeting, stabilizing and / or imaging agent delivery applications.

A bone targeted alkaline phosphatase comprising a polypeptide having the structure: Z-sALP-Y-spacer-X-Wn-V, wherein sALP is the extracellular domain of the alkaline phosphatase; wherein V is absent or is an amino acid sequence of at least one amino acid; X is absent or is an amino acid sequence of at least one amino acid; Y is absent or is an amino acid sequence of at least one amino acid; Z is absent or is an amino acid sequence of at least one amino acid; and Wn is a polyaspartate or a polyglutamate wherein n=10 to 16. Kits and methods of use thereof.

The present invention provides one kind of gamma-polyglutamic acid generating bacterium and the process of preparing gamma-polyglutamic acid (gamma-PGA) therewith. The gamma-polyglutamic acid generating bacterium is Bacillus substilis CCTCC M 206102. The preparation process of gamma-PGA includes using Bacillus substilis CCTCC M 206102 as fermenting strain, and shake flask fermentation of sugar material without adding L-glutamic acid. The present invention has relatively low material cost, gamma-PGA output up to 18g / L and excellent industrial application foreground.

The invention particularly discloses a composition for promoting opening of skin aquaporin, which is mainly proportionally prepared from deionized water, glycerol, carbomer, xanthan gum, lycine, trehalose, inositol, taurine, lauryl mammary gland sodium lactate, monomethyl-monosilane triol, oxyproline, aspartic acid, yeast amino acid, polyglutamic acid, sodium hyaluronate, dipotassium glycyrrhetate, purslane extracting solution, licorice extract, ceramide, phytosphingosine, cholesterol, oligopeptide-1, polyquaternary amine salt-14 and the like. The composition has the function of promoting opening of skin aquaporin, has better water replenishing and locking effect, and effectively prevents and resists the phenomena of dry skin mucosa and skin aging.

Disclosed are water soluble compositions of paclitaxel and docetaxel formed by conjugating the paclitaxel or docetaxel to a water soluble polymer such as poly-glutamic acid, poly-aspartic acid or poly-lysine. Also disclosed are methods of using the compositions for treatment of tumors, auto-immune disorders such as rheumatoid arthritis. Other embodiments include the coating of implantable stents for prevention of restenosis.

Compositions are proposed which comprise folic acid in combination with 5-methyltetrahydrofolic acid, and also compositions which comprise folic acid, 5-methyltetrahydrofolic acid and / or 5-methyltetrahydrofolylpolyglutamate and also comprise a dietary component and / or a nutrient preparation, and their use is also proposed.

A process for preparing gamma-polyglutamic acid and polyglutamate includes such steps as culturing bacillus, such as Bacillus subtilis and lichen bacillus, in the culture medium containing carbon source, nitrogen source and Glu to obtain high-activity (1-10 U / ml) gamma-glutamyl transpeptidase, preparing high-concentration fermented liquid of gamma-polyglutamic acid, and solvent precipitation or chemical precipitation.

Various biodegradable polyglutamate polymer conjugates that can include recurring units of the general formulae (I) and (II) are described herein. Such polymer conjugates are useful for variety of drug, targeting, stabilizing and / or imaging agent delivery applications.

The invention provides a preparation method of a gamma-polyglutamic acid / Pulullan composite hydrogel. The hydrogel contains gamma-polyglutamic acid, Pulullan and water. The preparation method comprises the following steps: dissolving 8-20% of gamma-polyglutamic acid in distilled water to obtain a first reaction solution; adding Pulullan according to different gamma-polyglutamic acid / Pulullan ratios to obtain a second reaction solution; and adding a crosslinking agent into the second reaction solution, stirring uniformly, regulating the pH value of the system, and reacting at 50 DEG C for 24 hours. The gamma-polyglutamic acid / Pulullan composite hydrogel prepared by the method has favorable adsorbability for brine. The hydrogel is applicable to a brine environment, and is especially applicable to an environment of soil, sweat, blood or the like.

The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one protein drug or bioactive agent characterized with a positive surface charge and their enhanced permeability for paracellular protein drug and bioactive agent delivery.

Methods for preparing and isolating polymer conjugates that include a recurring unit of Formulae (I) and (Ia) are described herein. The polymer conjugates can include an anti-cancer drug.

The bacillus subtilis NX-2, or CGMCC NO.0833 is one bacillus spawn obtained through screening and is used for the production of gamma-polyglutamic acid and its salt as well as glutathione and its precursor. In optimized condition, gamma-polyglutamic acid may be accumulated to 30-50 g / L and produced in the production efficinecy of 0.8-2.5 g / L. When bacillus subtilis NX-2 is used in fermenting to produce gamma-glutamyl transpeptidase in optimized condition, the fermented liquid may reach enzyme activity of 1-5 U / ml; and the fermented liquid or separation obtained enzyme or the immobilized enzyme is used in the production of glutathione and its precursor with substrate dipeptide or dipeptide precursor converting rate of 30-80% being reached.

The invention discloses a gamma-polyglutamic acid producing bacterium. The gamma-polyglutamic acid producing bacterium is classified and named as Bacillus Subtilis hzy-1, has been deposited in the Chinese Industry Culture Collection Center with the accession number of CCTCC M 2018226 in May 3, 2018. Meanwhile, the invention further discloses a method for efficiently synthesizing gamma-polyglutamicacid. Compared with an existing technology for producing gamma-polyglutamic acid, the method has the advantages that by a used bacillus subtilis strain, the gamma-polyglutamic acid can be effectivelyfermented and produced, the molecular weight of the produced gamma-polyglutamic acid is 1000 KDa or above, by optimization of bacillus subtilis strain culture conditions, the content of the gamma-polyglutamic acid in fermentation liquor reaches 40-60 g / L, and therefore, a method for effectively preparing the gamma-polyglutamic acid at low price is provided. The produced gamma-polyglutamic acid can be applied to the fields of agriculture, cosmetics and the like.

The invention discloses bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof, and belongs to the technical field of fermentation of microorganisms. In the invention, a bacterial strain YXY-C1 is used for preparing the gamma-polyglutamic acid efficiently by separation; and by using cow dung and monosodium glutamate meal as main raw materials and bran, corn meal, bean meal, citric acid, MgSO4.7H2O and MnSO4.H2O as auxiliary materials, the bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by solid fermentation, the yield is 39 grams per kilogram of culture medium, and the molecular weight is 300kD. The bacterial strain YXY-C1 can prepare the gamma-polyglutamic acid by fermenting solid wastes and low-price agricultural and sideline products, and has the advantages of low raw material cost, simple method, high yield, convenient product separation and purification and potential application prospect.

The invention discloses a pharmaceutical composition of bioactive nanoparticles composed of chitosan, poly-glutamic acid, and a bioactive agent for oral delivery. The chitosan-based nanoparticles are characterized with a positive surface charge and enhanced permeability for oral drug delivery.

The present invention relates to a moisturizer comprising γ-polyglutamic acid (γ-PGA, H form), and / or one or more of its salts (i.e., γ-polyglutamate in Na+ form, γ-polyglutamate in K+ form, γ-polyglutamate in NH4+ form, γ-polyglutamate in Mg++ form, γ-polyglutamate in Ca++ form) and / or γ-polyglutamate hydrogel, wherein said moisturizer is used in cosmetic products or personal care products.

The invention provides a method for cleanly producing glutamic acid, gamma-polyglutamic acid and an organic fertilizer, relating to a method for sequentially preparing the glutamic acid, the gamma-polyglutamic acid and an organic slow-release fertilizer with functions of retaining water and humidity by adopting a microbiological fermentation method. According to the method provided by the invention, the gamma-polyglutamic acid is produced by using electric waste liquid such as the glutamic acid; the extraction rate of the glutamic acid is only 70-80 percent; the gamma-polyglutamic acid, a large amount of mycoprotein and the organic slow-release fertilizer with functions of retaining water and humidity, with higher added values, are simultaneously obtained; an extraction mother solution of the glutamic acid is simply adjusted to be used as a culture medium for producing the gamma-polyglutamic acid, so that a hybridization process is omitted and the discharge amount of waste water in the extraction process of the glutamic acid is reduced and zero discharge is basically realized; and the pressure of glutamic acid production enterprises on environment pollution is reduced and the aim of clean production is achieved.

The invention discloses a preparation method of an amphipathic gama-polyglutanmic acid nanodrug carrier. The preparation method comprises the steps of grafting hydrophobicity group on gama--polyglutanmic acid serving as a main chain, thus obtaining amphipathic gama-polyglutanmic acid derivates with good biocompatibility and degradability, and self assembling in water medium to form nano micelle with particle size of 200-1000nm. The hydrophobic nuclei-hydrophilic shell structure is beneficial to improvement of solubility of hydrophobic drug in water medium, further possibly improves the bioavailability of drugs in the body, and also can be used for load of protein and DNA. The preparation method of the amphipathic gama-polyglutanmic acid nanodrug carrier is simple and practicable, all raw materials can be industrially produced; reproducibility is good; and the amphipathic gama-polyglutanmic acid nanodrug carrier has high drug loading rate, good sustained release effect and intelligent environment response (pH-enzyme complex sensitivity) and other corresponding characteristics, as well as good popularization and application values.

A nanometer selenium nutritive liquid fertilizer is disclosed. The liquid fertilizer comprises amino acids, polyglutamic acid and nanometer selenium. The liquid fertilizer comprises following eighteen amino acids: 1.35% of aspartic acid, 0.49% of threonine, 0.57% of serine, 2.05% of glutamic acid, 0.94% of glycine, 0.75% of alanine, 0.65% of valine, 0.23% of methionine, 0.55% of isoleucine, 0.79% of leucine, 0.29% of tyrosine, 0.96% of phenylalanine, 0.81% of lysine, 0.45% of histidine, 1.48% of arginine, 0.62% of proline, 0.002% of tryptophan and 0.12% of cystine. The liquid fertilizer comprises the nanometer selenium, the polyglutamic acid, a fertilizer-retaining and water-retaining component PGA, proteins, saccharides, trace elements, and other nutrients. In rice cropping tests and under same fertilizing and managing conditions, the selenium content in a product reaches 0.522 mg / kg and is 11 times of the selenium content of a product obtained by spraying sodium selenite, and the liquid fertilizer increases the yield and improves quality. The liquid fertilizer is an ideal fertilizer for production of selenium-rich functional foods.

The invention relates to the technical field of medicines. The invention relates to the field of biomimetic drugs, in particular to biomimetic drug-loaded nanoparticles targeting brain tumor and a preparation method and application thereof, and particularly relates to adriamycin-loaded polyglutamic acid biomimetic nanoparticles which target brain tumor cells and is coated by human brain glioma U87cell membranes and a preparation method and application thereof in drugs for treating brain tumor diseases. The preparation method of the biomimetic drug-loaded nanoparticles targeting brain tumor comprises the following steps: preparing adriamycin-loaded polyglutamic acid nanoparticles, and coating the surfaces of the nanoparticles with the human brain glioma U87 cell membranes to form the stable biomimetic drug-loaded nanoparticles. Therefore, higher encapsulation efficiency and better slow release performance are realized, the targeting property of the nano drug delivery system is improved, the biomimetic drug delivery nanoparticles penetrating through the blood-brain barrier can be more effectively gathered in a brain tumor area, and the effect of treating brain tumors by chemotherapeutic drugs is improved.

The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one anti-hemophilic factor or bioactive agent characterized with a positive surface charge and their enhanced permeability in oral drug delivery.

The invention discloses a pharmaceutical composition of bioactive nanoparticles composed of chitosan, poly-glutamic acid, and a bioactive agent for oral delivery. The chitosan-based nanoparticles are characterized with a positive surface charge and enhanced permeability for oral drug delivery.

The invention discloses a preparation method and use of a thioctic acid-modified polyethylene glycol-polyaminoacid amphiphilic triblock copolymer. A hydrophilic chain of the amphipathic triblock polymer is polyethylene glycol, a middle hydrophobic chain segment of the amphipathic triblock polymer is poly(benzyl glutamate), a tail hydrophobic chain segment of the amphipathic triblock polymer is polyphenylalanine and the side chain of the middle chain segment is modified through thioctic acid. The amphipathic triblock polymer is self-assembled in water to form a polymer nanometer micelle with polyethylene glycol as an outer crown, thioctic acid-modified poly(benzyl glutamate) as a middle shell and polyphenylalanine as an inner core. Through crosslinking of the nanometer micelle, the stable reduction-sensitive shell crosslinked nanometer micelle is obtained so that the nanometer micelle is not easily dissociated in vitro and in blood and thus stability of a nanometer micelle-coated drug is guaranteed. When the nanometer micelle enters a tumor cell, the nanometer micelle can be fast crosslinked and dissociated so that the drug can be fast released and produces high treatment effects. The amphipathic triblock polymer solves the problems of drug leakage in vivo, low conveying efficiency and slow release in cells.

Sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaaminopterin is assessed and patients are selected for treatment of cancer with 10-propargyl-10-deazaaminopterin, by determining the amount of a selected polypeptide expressed by the cancer and comparing the amount with the amount of the selected polypeptide expressed by a reference cancer. The polypeptide includes a member of a folate pathway polypeptide within a cell and may include at least one of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT).

The invention relates to a preparation method of beta-cyclodextrin / polyglutamic acid modified biochar and application of the biochar. An adsorbent adopts biochar as a matrix, and beta-cyclodextrin / polyglutamic acid is grafted on the matrix. The preparation method includes the specific steps that beta-cyclodextrin / polyglutamic acid is prepared and compounded firstly, then biomass dried in the sun and ground into powder is calcined at high temperature, and after the biochar is prepared, beta-cyclodextrin / polyglutamic acid is grafted on the surface of the biochar. The application includes the steps that the modified biochar is added to 50 mL of hexavalent chromium wastewater with the concentration of 20-800 mg / L, the use amount of the biochar is 10 mg, after a vibration adsorption reaction is conducted for a period of time at the pH value of 2-9 and the temperature of 20-50 DEG C, the adsorbent is collected through filtering separation, and hexavalent chromium ions in the wastewater is removed. The preparation method has the advantages that cost is low, the process is simple, adsorption performance is high, waste is effectively utilized and easy to separate, and environment friendliness is achieved.

The invention provides a polymer having a structure shown in formula (I), a glucose nano gel having a structure shown in formula (II), a preparation method of the polymer having the structure shown in the formula (I), a preparation method of the glucose nano gel having the structure shown in the formula (II) and a glucose nano gel composition. The glucose nano gel having the structure shown in the formula (II) is prepared by reacting polymers containing methoxy polyethylene glycol hydrophilic chain segments and polyglutamate hydrophobic chain segments under the action of a boric acid derivative crosslinking agent, wherein both the methoxy polyethylene glycol and the polyglutamate have favorable biocompatibility, and the polyglutamate has favorable biodegradability, thereby ensuring that the glucose nano gel having the structure shown in the formula (II) has favorable biocompatibility and biodegradability; and boric acid groups in the boric acid derivative crosslinking agent are sensitive to glucose and can quickly respond to the concentration change of the glucose, thereby being beneficial to the quick release of medicaments and enhancing the curative effect of the medicaments. The formula (II) is shown in the specification.