The invention discloses a calibration device used for medical radiation. The calibration device comprises a base, fixing rods and a vertical plate, wherein a support base is fixed above the base, a support rod is arranged above the support base, a telescopic rod is mounted at the upper part of the support rod, brackets are fixed to the outer sides of the telescopic rod, threaded rods are in threaded connection to the lower parts of the fixing rods, the fixing rods are positioned below the brackets, laser lamps are mounted at the lower parts of the threaded rods, a chain take-up wheel is mounted above the vertical plate, and the vertical plate is positioned on the right side of the base. For the calibration device, due to the arrangement of balls, a cross frame can perform circular rotation by taking the support rod as the center shaft, by utilizing the size match between the cross frame and adjusting frames, the adjusting frames can be inserted in or pulled out on the cross frame, theplacing positions and placing directions of the adjusting frames are flexibly changed, thus the accuracy during calibration is improved, outer protection rings are rotatably connected with the adjusting frames through connecting rods, the placing angles, relative to the outer protection rings, of condensing lenses is adjusted, and thus the direction of laser is adjusted.