A method, an
electrode, a measuring
cell, and a measuring device are disclosed which can detect and quantitatively determine an
analyte having specific bonding properties, in a
highly sensitive, simple and accurate manner using
photocurrent. This method comprises contacting a
working electrode and a counter
electrode with an
electrolyte medium, wherein the
working electrode has an
analyte immobilized thereon through a probe substance and wherein the
analyte is bonded to a sensitizing dye; irradiating the
working electrode with light to photoexcite the sensitizing dye; and detecting
photocurrent flowing between the working
electrode and the counter electrode, wherein the
photocurrent is generated by transfer of electrons from the photoexcited sensitizing dye to the working electrode. The working electrode comprises an
electron accepting layer comprising an
electron accepting substance capable of accepting electrons released from the sensitizing dye in response to
photoexcitation, wherein the probe substance is supported on a surface of the
electron accepting layer. The electron accepting substance is an
oxide semiconductor having an
energy level lower than that of a lowest unoccupied molecular
orbit (LUMO) of the sensitizing dye. The
electrolyte medium comprises an
electrolyte and at least one
solvent selected from an aprotic
solvent and a
protic solvent, wherein the electrolyte comprises a salt capable of providing an oxidized sensitizing dye with electrons.