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184 results about "Light delivery" patented technology

Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.
Owner:WEISS ROBERT B +6

Method of calibrating light delivery systems, light delivery systems and radiometer for use therewith

In a method of calibrating a light delivery device (10) having a solid state light source (12), for example comprising LEDs of an LED array, and an intensity control unit (16) comprising LED array driver and a dimmer module for generating a control signal for controlling at least the intensity of the light source, the light source is temporarily connected by a light guide (24; 24, 26) to a radiometer (38) for detecting irradiance of the delivered light. The light delivery device has a memory (30) for storing control signal parameters and associated radiance levels. The light delivery device is calibrated by adjusting the control signal parameters, e.g. a PWM duty cycle of a control signal to each of a series of predetermined settings, obtaining from the radiometer a corresponding series of delivered light irradiance levels measured thereby, storing the irradiance levels and associated control signal parameters in memory, and applying a best fit algorithm to the irradiance measurements and control signal parameters. Thereafter, a desired irradiate level can be set by selecting the best fit control signal parameters, such as duty cycle of a PWM control or other parameters. Output intensity levels may be measured at the same time as the irradiance levels and used to compensate for light source output level changes when setting a desired irradiance level.
Owner:EXCELITAS CANADA
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