A slide imaging device (110) for digital morphology in
hematology is disclosed, comprising at least one z-axis stage (112) configured to place a
microscope slide (114) at a
sample plane, wherein a biological sample may be placed on the
microscope slide (114), wherein the z-axis stage (112) is configured to set a relative distance along the
optical axis (118) of the slide imaging device (110) between the
microscope slide (114) and the imaging
system (116) of the slide imaging device (110). The slide imaging apparatus (110) further includes at least one sample beam path and at least one
autofocus beam path, the at least one
autofocus beam path including at least one pattern illumination source (132), the at least one pattern illumination source including at least one
light emitter (134) and at least one
grating (136), wherein the
grating (136) is tilted relative to the
optical axis (118) of the slide imaging apparatus (110), wherein the
autofocus beam path includes at least one second
transmission system (140) and at least one autofocus camera (138), wherein the second
transmission system (140) is configured to allow a pattern generated by the pattern illumination source (132) to be projected onto the
sample plane and to allow a pattern reflected from the
sample plane to be propagated to the autofocus camera (138), wherein the autofocus camera (138) is configured to generate at least one digital autofocus image of the pattern reflected from the sample plane. The slide imaging apparatus (110) further includes at least one
processing unit (148) configured to analyze the digitally autofocused image, wherein the analysis includes
spatial analysis of contrast and determination of the image location with maximum contrast. The slide imaging apparatus (110) further includes at least one
control unit (150) configured to control the z-axis stage (112) taking into account the determined image location to position the
microscope slide (114) along the
optical axis (118) of the slide imaging apparatus (110).