The invention discloses a high-speed multicolor and multimode
structured light illumination super-resolution microimaging
system.
Laser generated by a multicolor
light source is incident into a high-speed gating switching module; the high-speed gating switching module selects the
laser in one or multiple colors and enables the
laser to irradiate a
structured light generation and modulation module; the
structured light generation and modulation module generates periodically modulated structured light and controls the direction and the phase of the structured light; the modulated
laser is transmitted to a polarization control module; the polarization control module adjusts the polarization direction of the laser, so that the structured light illumination stripe is high in contrast, and the laser is transmitted to a space filter module; the space filter module filters out redundant
stray light, and then emits the laser to a sample module through a
fluorescence module and an objective lens in sequence;
signal light emitted by the sample module is collected by the objective lens, and then the
fluorescence module separates exciting light from the
signal light; finally the
signal light is received by a detection module. The
system is high in structured light illumination stripe contrast and high in imaging speed, and meanwhile at least 5-
color imaging can achieve the optimal performance.