The invention relates to the technical field of raw silk inspection, in particular to a method for simultaneously identifying feed silkworm rearing raw silk,
mulberry leaf silkworm rearing raw silk and fresh cocoon raw silk / dry cocoon raw silk, which comprises the following steps:
grinding the feed silkworm rearing raw silk,
mulberry leaf silkworm rearing raw silk, fresh cocoon raw silk and dry cocoon raw silk from the same source, adding equal amount of
hydrochloric acid after
grinding, mixing and reacting, stirring by using a glass rod, and standing for 24 hours; then carrying out ultrasonic treatment and
centrifugation, and removing supernate; removing the supernatant, adding the same amount of deionized water, stirring, washing, standing, pouring out the supernatant, repeating for several times, centrifuging, taking the precipitate,
drying,
grinding and crushing to obtain an extract; and determining the obtained extract by adopting a
stable isotope ratio mass spectrometer, and judging the silkworm rearing raw silk of the feed, the silkworm rearing raw silk of the mulberry leaves, the fresh cocoon raw silk and the dried cocoon raw silk according to the N content and the 15N relative content. The method for detecting the total N content and the < 15 > N relative content in the raw silk is reliable, high in recognition rate, visual and reliable in experimental result and high in accuracy.