The invention discloses a vein needle conduit assembling machine, comprising a rack and a conveying device for conveying the vein needle conduit assemblies. A straw assembly mechanism, a needle seat feeding mechanism, an internal glue adding assembly, a needle handle feeding mechanism, a drying mechanism, a conicity detection device, a conduit ventilation detection device, a pulling force detection device and a conduit unloading device are successively arranged on the rack from the left to the right and are connected through the conveying device; and the straw assembly mechanism is connected to a conduit storage mechanism. The vein needle conduit assembling machine can finish whole vein needle conduit assembly and does not need artificial contact on the vein needle assemblies, so that the vein needle is not easy to get pathogen during a packaging process and safety can be guaranteed. Besides, the vein needle conduit assembling machine is high in automation degree, high in assembling efficiency, beneficial for mass production, reasonable in design, simple in structure and strong in practicability.