This invention discloses a multimodal super-resolution
microscopy imaging
system and method based on multifocal
structured light illumination, belonging to the field of optical
microscopy imaging technology. The
system includes: a
laser beam generation module for generating and controlling the
spatial coherence of the illumination beam; a multifocal
structured light excitation module, including a beam modulation device, which modulates the illumination beam into a multifocal
structured light array and projects it onto the
sample plane to excite the sample to generate interference scattering signals and / or
fluorescence signals; an interference scattering
signal detection module for detecting interference scattering signals; a
fluorescence signal detection module for acquiring
fluorescence signals; and a
control system module electrically connected to the beam modulation device, the interference scattering
signal detection module, and the fluorescence signal detection module, respectively, for controlling the generation of multifocal illumination and its synchronization with
signal acquisition. This invention solves the problem that existing interference scattering
microscopy techniques cannot simultaneously achieve high speed, high signal-to-
noise ratio, and high-resolution imaging.