High-speed multicolor and multimode structured light illumination super-resolution microimaging system and method thereof

Abstract

The invention discloses a high-speed multicolor and multimode structured light illumination super-resolution microimaging system. Laser generated by a multicolor light source is incident into a high-speed gating switching module; the high-speed gating switching module selects the laser in one or multiple colors and enables the laser to irradiate a structured light generation and modulation module; the structured light generation and modulation module generates periodically modulated structured light and controls the direction and the phase of the structured light; the modulated laser is transmitted to a polarization control module; the polarization control module adjusts the polarization direction of the laser, so that the structured light illumination stripe is high in contrast, and the laser is transmitted to a space filter module; the space filter module filters out redundant stray light, and then emits the laser to a sample module through a fluorescence module and an objective lens in sequence; signal light emitted by the sample module is collected by the objective lens, and then the fluorescence module separates exciting light from the signal light; finally the signal light is received by a detection module. The system is high in structured light illumination stripe contrast and high in imaging speed, and meanwhile at least 5-color imaging can achieve the optimal performance.

Description

technical field [0001] The invention relates to the field of microscope imaging, in particular to a high-speed multi-color multi-mode structured light illumination super-resolution micro-imaging system and method. Background technique [0002] The optical microscope has greatly promoted the development of biology since its invention in the 17th century. As early as 1665, human beings first discovered, described and defined cells, the basic unit of life activities, with the help of optical microscopes, thus starting the transformation of biological research from macroscopic description to microscopic quantification. It can be said that the advancement of optical microscopy, especially the advancement of fluorescence microscopy technology, is an extremely important driving force for the rapid development of life science research in recent decades. But the fluctuating nature of light is known to limit the resolution of light microscopy. As early as more than 100 years ago, th...

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Problems solved by technology

[0024] In order to overcome the shortcomings of the existing structured light illumination microscope system, such as slow original image acquisition speed, difficulty in real-time optimization of imaging conditions for excitation light of any wavelength, low contrast of structured light illumination stripes, and inability to flexibly switch between different imaging modes, etc., The invention provides a high-speed multi-color multi-mode structured light illumination super-resolution microscopic imaging system and method

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