89 results about "Science research" patented technology

The invention discloses a research process planning method and device, electronic equipment and a readable storage medium, and relates to the technical field of scientific research, and the method comprises the steps: carrying out the understanding analysis of key research information, carrying out the article retrieval according to a determined search phrase, and obtaining a target article set, and determining a target research direction according to the key research information and the target article set after the structured hierarchical representation, and planning a research process based on the target research direction, so that the problem that the research process is difficult to carry out during literature review and research process planning in the professional technical field can be solved. The scientific research process planning method solves the technical problems that the scientific research process planning method depends on manual retrieval and analysis of researchers and has high professional quality requirements on the researchers, and the technical effects of improving the intelligence degree and efficiency of scientific research process planning, providing comprehensive and accurate research support for the researchers, accelerating the scientific research process and improving the scientific research efficiency and quality are achieved.

The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and / or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and / or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides a variety of configurations of the RNA prime editors each comprising a nucleic acid programmable RNA binding proteins (napRNAbp), such as Cas13, and an RNA-dependent RNA polymerase (RDRP), which are provided as fusion proteins or which can be separately provided in trans. The RNA prime editors are guided to a target RNA site by a guide RNA, which can be a rpegRNA that includes a template region for the synthesis of an RNA sequence to be installed on the RNA molecule attached to an available 3′ terminus. In others embodiments, the RNA template can be provided in trans.

The invention relates to the technical field of social humanistic science research, in particular to an engineer engineering skill proficiency evaluation method, which comprises the following steps: identifying the core skill category of an engineer, collecting the skill level input of the engineer to establish a personnel skill inventory, generating original skill data input, and inputting based on the original skill data. According to the method, through identification of core skill categories of engineers and collection of skill level input, and in combination with structural analysis of task on-time completion rate, a direct mapping relation is generated between the engineer ability and actual performance data, and the deviation that ability evaluation depends on subjective impressions singly is avoided. On the basis of clear division of project stages and process nodes, the change trend of skill requirements is quantized into skill numerical value weights in different stages, and skill evaluation has time sequence dynamics by extracting associated information between the stages and the skill.

The present invention belongs to the field of aquatic biological breeding, and specifically relates to a method and application of creating germplasm without intermuscular spines of economic fish for breeding. runx2b The economic fish, Megalobrama amblycephala, is gene-edited using a gene as the target gene. F0 generation fish with mutations are selected and cultured as parent fish. The F1 generation is produced by mating and breeding the F0 generation mutant male and female parents. F1 fish without intermuscular spines are screened and cultured to adult fish for use in cultivating a new germplasm of Megalobrama amblycephala without intermuscular spines. The present invention utilizes a gene mutation method to obtain a new germplasm of the economically cultivated fish, Megalobrama amblycephala, without intermuscular spines. This provides an improved method for solving the problem of the significant impact of intermuscular spines in the muscles of important aquacultured economic fish such as silver carp, bighead carp, carp, crucian carp, and bream in my country on their consumption and processing. It also provides a basis for subsequent research on the molecular formation mechanism of intermuscular spines, and has important scientific research value and industrial application value.

The invention discloses a scientific research thought generation method based on multiple agents, and belongs to the technical field of agent application. The invention aims to solve the problems that the existing multi-agent scientific research cannot simulate the random sequence of scientific discussion and does not reflect the diversity of the agents. Comprising the steps that each scientist generates a scientific research theme according to theme prompt words, and a leader determines a final scientific research theme; under the scientific research theme, each scientist generates an initial thought according to a knowledge base of the scientist and a first prompt word, and other scientists modify the initial thought according to a second prompt word to obtain a modified thought; the leader summarizes all the revised ideas to obtain revising suggestions, the revising suggestions are returned to the initial scientists for reflection, and the final idea of each scientist is obtained; and the innovation of the final idea of each scientist is checked to determine the final scientific research idea. The method is used for automatically generating scientific research ideas.

The invention provides a user personalized scientific research planning scheme generation method and system based on multi-agent interaction. The method comprises the following steps: firstly, receiving a question of a user, outputting a keyword through a keyword disassembling agent, further generating a related expert agent, integrating literature information, forming a theme through interactive discussion between the user and the multiple agents, and storing the theme into a global memory library; after a user selects a theme, entering an interactive inspiration and approval stage, generating an initial scheme by a scientific research scheme planning agent, and constructing a feasible literature reference database through second correlation screening; the keyword generation agent is combined with the database to generate literature keywords, a final combination is obtained through exchange and mutation operation, scheme feasibility is enhanced through the detailed agent, evaluation is conducted through the review agent, if optimization is needed, evaluation is conducted again after updating is conducted through the optimization agent, and if improvement is not needed, a final scheme is output. The framework links are compact, and abundant, efficient and accurate scientific research intelligent reply and planning schemes can be provided for users.

The invention provides a Wuzhishan pig organ multi-omics data space integration and analysis method, and belongs to the technical field of data statistics and analysis, the method comprises the following steps: S1, experiment design and Wuzhishan pig organ sample standardization preparation; s2, independently collecting and preprocessing multi-omics data of Wuzhishan pig organs; s3, multi-omics data standardization and batch effect correction; s4, labeling and calibrating spatial dimension information; s5, spatial specificity correlation modeling of the multi-omics data is carried out; s6, carrying out integrated clustering analysis on the spatial multi-omics data; s7, performing function annotation and path enrichment analysis on the clustering feature clusters; and S8, analyzing the spatial specific molecular mechanism and constructing a multi-omics data spatial integration database. According to the method, spatialization and systematization analysis of the Wuzhishan pig organ multi-omics data is achieved through collaborative optimization of the HHO-IK-means-BI three algorithms, and technical support is provided for experimental zoology research and conversion of medical application.

This invention provides a molecular breeding method for fish with blunted and thickened intermuscular spines, belonging to the fields of gene editing and aquatic organism breeding technology. This invention utilizes CRISPR / Cas9 gene editing technology to mutate the bmp8a gene in fish, thereby obtaining fish with blunted and thickened intermuscular spines. The method of this invention is simple and easy to implement. Verification has shown that this invention can obtain a large number of stably heritable mutant zebrafish through gene knockout, and also provides an important reference for the precise design breeding of other farmed fish. It can serve as an efficient technical means to obtain new germplasm of fish with blunted and thickened intermuscular spines, possessing significant scientific research and industrial application value.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for identifying silver pheasant species and application thereof, and relates to the technical field of biology. The SNP marker can effectively solve the current identification problem caused by the problem that the silver pheasants are hybridized, nestlings and female pheasants are difficult to distinguish, and provides solid technical support for silver pheasant protection, population monitoring and related scientific research.

This invention provides a non-cytotoxic, in vivo compatible proximity labeling method for peroxidases, Hi-APEX, and its applications, belonging to the field of biotechnology. This invention provides a method for labeling interacting proteins, neighboring proteins, and / or neighboring RNA. By introducing TP probes, it completely overcomes the core limitation of peroxidase dependence on H2O2 without sacrificing the original high spatiotemporal resolution. The method provided by this invention exhibits significantly superior technical effects compared to existing technologies in terms of reduced toxicity, enabling in vivo application, precise analysis of redox-sensitive processes, dynamic tracking of protein transport, and simultaneous spatial multi-omics analysis. It provides a powerful tool for life science research and drug development, possessing significant scientific value and broad application prospects.

The invention is a registry in the cloud that stores metadata and quality control metadata for scientific studies and datasets that it has profiled with an AI-based quality control engine that runs in the data contributor's environment.

The invention discloses a photo-induced phase change protein and application thereof. The protein with the phase change function is provided for the first time, and the amino acid sequence of the protein is shown as SEQ ID NO.7; the invention further provides the IDR polypeptide with the phase change function, and the amino acid sequence of the IDR polypeptide is shown as SEQ ID NO.5. The invention also provides an application of the PE1 protein in photo-induced phase transition. The tool has the advantages of being high in space-time precision, good in biocompatibility, capable of integrating physiological signals and the like, can be used for controllable phase separation research, local enrichment, material construction and the like, and has wide prospects in the fields of life science research, drug development and synthetic biology.

A full-automatic spin coating and baking apparatus for scientific research is provided. The apparatus includes an apparatus body, first and second loading devices, a manipulator device, a spin coater, a baking device, a pipetting device, a vision device and a control device. The control device is configured to control the manipulator device to grasp a piece of wafer based on the positioning recognition of the vision device, place the grasped piece of wafer at a vacuum adsorption hole of the spin coater, and then cooperate with the pipetting device to aspirate and drip the photoresist onto the piece of wafer; control the spin coater to perform spin coating on the piece of wafer; control the manipulator device to grasp and place the piece of wafer on the baking device to bake; and control the manipulator device to place the piece of wafer back into the first loading device after baking.

This invention discloses a cultivation experimental device for studying plant ectomycorrhizal fungal interactions. It includes a base with a petri dish on it. A protective shell is installed around the petri dish on the top of the base. A circular plate is rotatably mounted inside the protective shell. Both the circular plate and the top surface of the protective shell have two symmetrical arc-shaped ventilation openings. An adjustment component connects the circular plate and the protective shell, including a splined sleeve rotatably connected to the top of the protective shell. This invention provides a cultivation experimental device for studying plant ectomycorrhizal fungal interactions that offers high adjustment precision, effortless operation, and stability. The precise design of the ventilation opening adjustment component allows for accurate control of the opening size, meeting the oxygen requirements of different fungal species and plants. Furthermore, the threaded connection of the protective shell enhances the stability and practicality of the device, providing strong support for scientific research.

The invention provides a mechanism-data fusion driven hydration dynamics modeling prediction method and device, and the method comprises the steps: improving the prediction precision of the spatio-temporal evolution of a hydration microstructure through the deep fusion of mechanism calculation and experimental data; the physical interpretability is kept by adopting a mechanism model, mechanism-experiment differences are automatically corrected through a deep learning network, the dependence of a pure data driving method on large-scale training data and the limitation of the mechanism model in the aspects of parameters and hypothesis are overcome, hydration rate characteristics at different moments are considered, the simulation efficiency and accuracy are improved, and the method is suitable for popularization and application. The method is high in environmental adaptability, provides technical support with higher universality and reliability for later scale prediction and performance evaluation, and provides powerful guarantee for more comprehensively mastering the cement hydration rule in engineering application and scientific research.

The invention relates to the technical field of scientific research of micropaleontology fossils, in particular to a dripping and dissolving device for radioactive insect fossil treatment. The dripping and dissolving device comprises a comprehensive experiment table, a device box, a rock block placing module and a dripping bottle, the comprehensive experiment table comprises a base, a first vertical rod is arranged on the base, and a liquid dropping bottle support assembly is fixed to the first vertical rod. A second vertical rod is further included, and the second vertical rod is sleeved with a third vertical rod so that the third vertical rod can rotate left and right. The other end of the third vertical rod is connected with a fourth vertical rod and a magnifying lens connected with the fourth vertical rod. A device box is arranged on the base, and a rock block placing module and a hanging lug type screen are arranged in the device box. According to the dripping and dissolving device, the dripping speed and the corrosion position are accurately regulated and controlled, complete extraction and in-situ observation of fossils are guaranteed, surrounding rocks are effectively removed, and the extraction efficiency of important fossils is improved. According to the dripping and dissolving device, technical support is provided for radioactive insect fossil research, and the safety design is greatly enhanced.

1. Name of the Design Product: Wheeled-Legged Robot. 2. Use of the Design Product: For search and rescue, logistics distribution, and scientific research. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photo Most Illustrating the Design Key Points: Perspective View 1.

The invention belongs to the technical field of gene editing and disease model construction, and particularly relates to a construction method and application of a Wilson disease animal model. Aiming at a large fragment deletion mutation type which exists clinically in Wilson disease but lacks a corresponding animal model, a CRISPR / Cas9 gene editing technology is utilized, a pair of sgRNAs is specifically designed, an eighth exon region of a mouse Atp7b gene is precisely cut and deleted, and a WD mouse model with deletion mutation (c.2333340delGACGGTGG) of eight basic groups of the eighth exon of the Atp7b gene is successfully constructed. The method can be used for research on pathogenesis of WD, screening of novel diagnostic markers and evaluation of curative effect of therapeutic drugs, is particularly suitable for preclinical evaluation of gene therapy strategies and development of adjuvant therapeutic drugs of targeted NLRP3 inflammasomes, and has great scientific research value and clinical transformation prospect.

This invention provides the application of substances targeting the CYTB-187AA gene and / or protein in the preparation of drugs for delaying aging and / or prolonging lifespan. The research results of this invention indicate that by reducing the expression of CYTB-187AA protein and / or inhibiting its activity, the aging process can be effectively delayed, thereby achieving the goal of extending healthy lifespan or lifespan. The research results of this invention are of great significance for scientific research on aging mechanisms and the development of anti-aging intervention strategies.

The invention discloses a method and a system for generating a plant profile and species retrieval table based on deep learning, and relates to the technical field of atlas construction. Sample data are collected, feature extraction is performed on the sample data, and a corresponding sample feature set is obtained; training a deep learning model according to the obtained sample feature set, generating plant content according to a training result, and constructing a plant information base; a species retrieval table is constructed, and the plants needing to be recognized are recognized through the species retrieval table; the plant content and the species retrieval table are automatically generated, new plant data can be continuously learned through the model, the plant content and the retrieval table are updated in real time, and along with discovery of new species and updating of data sample data, the model can automatically adapt to new features, update the retrieval table and expand the plant content. The function ensures that the plant species and the retrieval table can follow the progress of scientific research, so that botany researchers can remarkably improve the identification efficiency of the plant species.

The present application relates to the technical field of regulation system, specifically, a method and model for estimating the thermal risk of substitution chlorination reaction, comprising the following steps: step 1, classifying and sorting different types of substitution chlorination reaction, obtaining thermal risk data and key parameters of various reactions, the key parameters including reaction temperature T, reaction pressure P, reactant molecular weight M, and the distance D between special atoms and reaction site; step 2, analyzing the influence weight of key parameters on the thermal risk of substitution chlorination reaction, and determining the proportion of each parameter in the thermal risk calculation; step 3, based on the parameter influence weight, constructing the calculation formula of the thermal risk value R by weighted summation according to the scene; step 4, determining the reaction thermal risk grade according to the thermal risk value R. The present application can greatly reduce the thermal risk test of some unnecessary substitution chlorination reaction, reduce the waste of scientific research funds, and also reduce the risk in the test process.

The invention discloses a universal method for producing multi-sample SNP variation sites, and relates to the technical field of biological information. Comprising the following steps: establishing a BWA / samtools index for a reference genome; comparing the sample fastq to generate SAM and converting the SAM to BAM; carrying out sambamba sorting and de-duplication; the method comprises the following steps: adding ReadGroup to Picard; constructing a BAM index; the GATK generates gvcf and converts the gvcf into VCF; the index is compressed by bgzip / tabix (bgzip / tabix); and the MergeVcfs merges and summarizes the VCFs. The simple and general method for the multi-sample SNP variation sites has remarkable advantages in efficiency, accuracy, simple universality, expandability and the like, is expected to play an important role in multiple fields such as biological research, medical diagnosis, agricultural breeding and the like, and promotes technical progress and scientific research development in related fields.

The invention discloses a method and a device for detecting the energy metabolism level of cells and organoids. According to the detection method disclosed by the invention, the acidification rate and the oxygen consumption rate of the extracellular microenvironment are obtained through signal response of hydrogen ions and the dissolved oxygen sensing electrode, and meanwhile, a dynamic detection microcavity is constructed by utilizing a metabolism detection device, so that the sensitivity and the accuracy of measurement are improved. By adopting different drug intervention schemes, the energy metabolism pathway of the cells can be regulated and controlled, so that the evaluation of the two energy metabolism pathways of glycolysis and aerobic respiration of the cells is realized. The in-situ and high-sensitivity cell energy metabolism detection method and device are provided by detecting the concentration change of the specific analyte of the extracellular microenvironment, the method has the universality of two-dimensional and three-dimensional cell models, a brand new platform is provided for scientific research and drug effect screening based on an in-vitro model, and the application prospect is wide.

The invention provides sgRNA (small guide ribonucleic acid) and target DNA (deoxyribonucleic acid) for detecting the activity of SpCas9 D10A Nickase and a detection method. A reaction system capable of expressing the function and a quantitative detection method (IEX-HPLC method) are established by simulating the enzyme activity function of a base editing enzyme incision, so that the requirements on experimental animals and cells are remarkably reduced, the detection period is shortened, and the 3R (reduction, optimization and substitution) principle of modern scientific research is met; meanwhile, the invention also provides an efficient and practical analysis method for the quality characteristic analysis work of the base editing enzyme, the convenience of quality detection in gene editing product production and research is improved, and the method has a large-scale commercial application prospect.

1. The name of the design product: intelligent fresh air system constant temperature cabinet. 2. The use of the design product: precise temperature and humidity control, active purification and air exchange for scientific research experiments in clinical medicine and animal medicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model discloses a multiphase composite micro-mixer combined with a shunting device. The multiphase composite micro-mixer comprises a mixing chip, a cover plate and an electromagnetic micro-valve, the mixing chip is provided with a mixing flow channel, and the mixing flow channel comprises a liquid inlet, a shunting and manufacturing time difference area, a mixing area and a liquid outlet which are communicated in sequence; the shunting and manufacturing time difference area comprises a shunting narrow channel and a shunting wide channel, the inner diameter of the shunting narrow channel is smaller than that of the shunting wide channel, an input port of the shunting narrow channel is communicated with an input port of the shunting wide channel, and an output port of the shunting narrow channel is communicated with an output port of the shunting wide channel; the cover plate seals and covers the mixing flow channel; the electromagnetic micro valve is used for controlling the opening and closing of the liquid outlet; the multi-phase quantitative mixing device has the advantages of high reliability, high mixing efficiency, wide application range and capability of performing multi-phase and quantitative mixing, and can play a greater role in scientific research and social life.

The invention discloses a domestic and overseas research and analysis system based on a large language model, and relates to the technical field of scientific research and analysis. Comprising an AI index system and a big language research and analysis model; the AI index system comprises a research development process index module and a related research achievement index module, and the large language research analysis model comprises a development process generation model and a research achievement architecture model; arranging development process contents through a development process generation model to generate a basic development process framework diagram; a related research result construction module is arranged on the development process framework diagram, and the indexed related research results and data are constructed on the development process framework diagram. Through the AI index system and the big language research and analysis model, the problems that existing analysis and research depend on summarization of professionals, and there is no more efficient and accurate analysis and research system are solved, efficient and accurate association combination and combination analysis of multiple fields can be achieved, and the problems that cross-field combination analysis is difficult and not high in precision are solved.

The invention belongs to the technical field of pharmaceutical co-crystals, and particularly relates to a synthetic method and application of an amantadine-butylphthalide co-crystal. Butylphthalide reacts to obtain butylphthalide salt or butylphenyltitanic acid, the butylphthalide salt or butylphenyltitanic acid reacts with different substituted amantadine or hydrochloride thereof to synthesize the pharmaceutical co-crystal, and the co-crystal structure can adjust the crystal lattice energy of the pharmaceutical, improve the solubility of the pharmaceutical in water or physiological media, promote the rapid dissolution and absorption of the pharmaceutical in vivo, improve the bioavailability and improve the bioavailability of the pharmaceutical. According to the eutecticum, the problem of solubility of butylphthalide is solved, the formed eutecticum shows certain neuroprotective activity, the biological activity of the eutecticum is remarkably superior to that of a monomer drug, the clinical medication dosage can be reduced while the same treatment effect is achieved, and the eutecticum has certain scientific research value and industrial popularization significance.

1. Name of the product in this design: Neurointerventional surgery simulator. 2. Application of this design: for use in medical teaching, training, and scientific research in neurointerventional surgery. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention belongs to the technical field of construction of crop research mapping populations, and particularly relates to a method for constructing a point mutation mapping population by a germ cold shock double-induction method, which comprises the following steps: selecting excellent dry seeds of an accurately sequenced cotton variety, and carrying out mutagenesis treatment twice to obtain secondary mutagenesis M0-generation seeds; continuously sowing seeds of M1-M3 generations of mutants, and carrying out point mutation detection; and according to the specific condition of point mutation detection, reasonable screening is performed to form a mutation mapping group. The novel method for constructing the point mutation mapping group is formed by utilizing radiation-induced point mutation as polymorphic sites generated by crop genomes, and has the advantages of simplicity, high efficiency, abundant sites, simple and quick construction process, time, manpower and material resource saving, easiness in popularization, capability of obtaining batch cotton mutants in a short time, and wide application prospect. Various single-character and multi-character mutation mapping groups are constructed, and material support is provided for scientific research.