46 results about "Science research" patented technology

This invention provides a non-cytotoxic, in vivo compatible proximity labeling method for peroxidases, Hi-APEX, and its applications, belonging to the field of biotechnology. This invention provides a method for labeling interacting proteins, neighboring proteins, and / or neighboring RNA. By introducing TP probes, it completely overcomes the core limitation of peroxidase dependence on H2O2 without sacrificing the original high spatiotemporal resolution. The method provided by this invention exhibits significantly superior technical effects compared to existing technologies in terms of reduced toxicity, enabling in vivo application, precise analysis of redox-sensitive processes, dynamic tracking of protein transport, and simultaneous spatial multi-omics analysis. It provides a powerful tool for life science research and drug development, possessing significant scientific value and broad application prospects.

The invention is a registry in the cloud that stores metadata and quality control metadata for scientific studies and datasets that it has profiled with an AI-based quality control engine that runs in the data contributor's environment.

The invention discloses a photo-induced phase change protein and application thereof. The protein with the phase change function is provided for the first time, and the amino acid sequence of the protein is shown as SEQ ID NO.7; the invention further provides the IDR polypeptide with the phase change function, and the amino acid sequence of the IDR polypeptide is shown as SEQ ID NO.5. The invention also provides an application of the PE1 protein in photo-induced phase transition. The tool has the advantages of being high in space-time precision, good in biocompatibility, capable of integrating physiological signals and the like, can be used for controllable phase separation research, local enrichment, material construction and the like, and has wide prospects in the fields of life science research, drug development and synthetic biology.

A full-automatic spin coating and baking apparatus for scientific research is provided. The apparatus includes an apparatus body, first and second loading devices, a manipulator device, a spin coater, a baking device, a pipetting device, a vision device and a control device. The control device is configured to control the manipulator device to grasp a piece of wafer based on the positioning recognition of the vision device, place the grasped piece of wafer at a vacuum adsorption hole of the spin coater, and then cooperate with the pipetting device to aspirate and drip the photoresist onto the piece of wafer; control the spin coater to perform spin coating on the piece of wafer; control the manipulator device to grasp and place the piece of wafer on the baking device to bake; and control the manipulator device to place the piece of wafer back into the first loading device after baking.

This invention discloses a cultivation experimental device for studying plant ectomycorrhizal fungal interactions. It includes a base with a petri dish on it. A protective shell is installed around the petri dish on the top of the base. A circular plate is rotatably mounted inside the protective shell. Both the circular plate and the top surface of the protective shell have two symmetrical arc-shaped ventilation openings. An adjustment component connects the circular plate and the protective shell, including a splined sleeve rotatably connected to the top of the protective shell. This invention provides a cultivation experimental device for studying plant ectomycorrhizal fungal interactions that offers high adjustment precision, effortless operation, and stability. The precise design of the ventilation opening adjustment component allows for accurate control of the opening size, meeting the oxygen requirements of different fungal species and plants. Furthermore, the threaded connection of the protective shell enhances the stability and practicality of the device, providing strong support for scientific research.

The invention provides a mechanism-data fusion driven hydration dynamics modeling prediction method and device, and the method comprises the steps: improving the prediction precision of the spatio-temporal evolution of a hydration microstructure through the deep fusion of mechanism calculation and experimental data; the physical interpretability is kept by adopting a mechanism model, mechanism-experiment differences are automatically corrected through a deep learning network, the dependence of a pure data driving method on large-scale training data and the limitation of the mechanism model in the aspects of parameters and hypothesis are overcome, hydration rate characteristics at different moments are considered, the simulation efficiency and accuracy are improved, and the method is suitable for popularization and application. The method is high in environmental adaptability, provides technical support with higher universality and reliability for later scale prediction and performance evaluation, and provides powerful guarantee for more comprehensively mastering the cement hydration rule in engineering application and scientific research.

The invention belongs to the technical field of gene editing and disease model construction, and particularly relates to a construction method and application of a Wilson disease animal model. Aiming at a large fragment deletion mutation type which exists clinically in Wilson disease but lacks a corresponding animal model, a CRISPR / Cas9 gene editing technology is utilized, a pair of sgRNAs is specifically designed, an eighth exon region of a mouse Atp7b gene is precisely cut and deleted, and a WD mouse model with deletion mutation (c.2333340delGACGGTGG) of eight basic groups of the eighth exon of the Atp7b gene is successfully constructed. The method can be used for research on pathogenesis of WD, screening of novel diagnostic markers and evaluation of curative effect of therapeutic drugs, is particularly suitable for preclinical evaluation of gene therapy strategies and development of adjuvant therapeutic drugs of targeted NLRP3 inflammasomes, and has great scientific research value and clinical transformation prospect.

This invention provides the application of substances targeting the CYTB-187AA gene and / or protein in the preparation of drugs for delaying aging and / or prolonging lifespan. The research results of this invention indicate that by reducing the expression of CYTB-187AA protein and / or inhibiting its activity, the aging process can be effectively delayed, thereby achieving the goal of extending healthy lifespan or lifespan. The research results of this invention are of great significance for scientific research on aging mechanisms and the development of anti-aging intervention strategies.

The present application relates to the technical field of regulation system, specifically, a method and model for estimating the thermal risk of substitution chlorination reaction, comprising the following steps: step 1, classifying and sorting different types of substitution chlorination reaction, obtaining thermal risk data and key parameters of various reactions, the key parameters including reaction temperature T, reaction pressure P, reactant molecular weight M, and the distance D between special atoms and reaction site; step 2, analyzing the influence weight of key parameters on the thermal risk of substitution chlorination reaction, and determining the proportion of each parameter in the thermal risk calculation; step 3, based on the parameter influence weight, constructing the calculation formula of the thermal risk value R by weighted summation according to the scene; step 4, determining the reaction thermal risk grade according to the thermal risk value R. The present application can greatly reduce the thermal risk test of some unnecessary substitution chlorination reaction, reduce the waste of scientific research funds, and also reduce the risk in the test process.

The invention discloses a method and a device for detecting the energy metabolism level of cells and organoids. According to the detection method disclosed by the invention, the acidification rate and the oxygen consumption rate of the extracellular microenvironment are obtained through signal response of hydrogen ions and the dissolved oxygen sensing electrode, and meanwhile, a dynamic detection microcavity is constructed by utilizing a metabolism detection device, so that the sensitivity and the accuracy of measurement are improved. By adopting different drug intervention schemes, the energy metabolism pathway of the cells can be regulated and controlled, so that the evaluation of the two energy metabolism pathways of glycolysis and aerobic respiration of the cells is realized. The in-situ and high-sensitivity cell energy metabolism detection method and device are provided by detecting the concentration change of the specific analyte of the extracellular microenvironment, the method has the universality of two-dimensional and three-dimensional cell models, a brand new platform is provided for scientific research and drug effect screening based on an in-vitro model, and the application prospect is wide.

The invention provides sgRNA (small guide ribonucleic acid) and target DNA (deoxyribonucleic acid) for detecting the activity of SpCas9 D10A Nickase and a detection method. A reaction system capable of expressing the function and a quantitative detection method (IEX-HPLC method) are established by simulating the enzyme activity function of a base editing enzyme incision, so that the requirements on experimental animals and cells are remarkably reduced, the detection period is shortened, and the 3R (reduction, optimization and substitution) principle of modern scientific research is met; meanwhile, the invention also provides an efficient and practical analysis method for the quality characteristic analysis work of the base editing enzyme, the convenience of quality detection in gene editing product production and research is improved, and the method has a large-scale commercial application prospect.

1. The name of the design product: intelligent fresh air system constant temperature cabinet. 2. The use of the design product: precise temperature and humidity control, active purification and air exchange for scientific research experiments in clinical medicine and animal medicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention belongs to the technical field of pharmaceutical co-crystals, and particularly relates to a synthetic method and application of an amantadine-butylphthalide co-crystal. Butylphthalide reacts to obtain butylphthalide salt or butylphenyltitanic acid, the butylphthalide salt or butylphenyltitanic acid reacts with different substituted amantadine or hydrochloride thereof to synthesize the pharmaceutical co-crystal, and the co-crystal structure can adjust the crystal lattice energy of the pharmaceutical, improve the solubility of the pharmaceutical in water or physiological media, promote the rapid dissolution and absorption of the pharmaceutical in vivo, improve the bioavailability and improve the bioavailability of the pharmaceutical. According to the eutecticum, the problem of solubility of butylphthalide is solved, the formed eutecticum shows certain neuroprotective activity, the biological activity of the eutecticum is remarkably superior to that of a monomer drug, the clinical medication dosage can be reduced while the same treatment effect is achieved, and the eutecticum has certain scientific research value and industrial popularization significance.

1. Name of the product in this design: Neurointerventional surgery simulator. 2. Application of this design: for use in medical teaching, training, and scientific research in neurointerventional surgery. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

1. The name of the design product: X-ray biological irradiator (160). 2. The use of the design product: life science research. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

The invention discloses a modularly assembled digital microscope which comprises an imaging system, a focusing system, a connecting system, a specimen system, a transmission system and a display storage processing system. According to the invention, modular design is adopted, each system can be independently disassembled and assembled, modular interfaces are unified and standardized, seamless cooperation among the modules is realized through a standardized communication protocol, and multiple functions of plug and play, hot plug and the like are supported. The overall power consumption is lower than 5W, and a mobile power supply is supported. The system has the advantages of being scientific, simple in structure, low in cost, capable of achieving remote monitoring and cooperative office, convenient to carry and use and the like. The invention has the advantages of safe and simple operation, light weight, environment-friendly and energy-saving selected materials and excellent effect. The system is suitable for popularization and application in non-professional scenes such as science popularization and innovation ability teaching of an education system and family livelihood practical detection. The system is especially suitable for various scenes such as scientific and technological innovation practice of students at school, teaching demonstration of teachers, scientific research, remote monitoring of dangerous places, cooperative office of operators and the like. The multi-purpose mirror is really realized and can be used anywhere.

The invention relates to the technical field of animal science data processing, and particularly discloses a feature derivation method and system for animal science original data. The method comprises the following steps: acquiring animal science original data, and constructing and storing a table structure in combination with data types, field meanings and user requirements; the method comprises the following steps: analyzing single-table column features and multi-table association relationships through data exploration, carrying out feature derivation based on an exploration conclusion, deeply mining data implicit association and internal laws, and converting the data implicit association and internal laws into quantifiable and explainable feature indexes. According to the scheme, the data quality is improved in a structured storage structured data form, the potential value of the data is released through a systematic feature derivation strategy, and the data information density and the analysis efficiency are enhanced. The technology breaks through the limitation of traditional data analysis, provides accurate and comprehensive data support for animal scientific research, assists in revealing essential rules and core mechanisms behind data, and lays a solid foundation for field theoretical innovation and practical application; the method is suitable for multiple directions in the animal science field and is wide in application range.

The application discloses a molecular marker and specific primers and an identification method for identifying Takifugu obscurus, Takifugu poecilonotus and hybrid Arlea japonica. The application can realize multiple test results through one-time PCR, and simultaneously complete the rapid identification of the three species of Takifugu obscurus, Takifugu poecilonotus and hybrid Arlea japonica. The application provides a fast and accurate identification method for Arlea japonica variety identification, solves the problems of difficult naked-eye differentiation and low operation accuracy of morphological feature differentiation, and realizes the characteristics of easy result judgment, high conservation, high accuracy and the like. The application provides technical support for variety identification and parent selection of the special economic fish, provides a train of thought for further carrying out genetic scientific research of hybrid Arlea japonica, and is favorable for promoting the vigorous development of the hybrid Arlea japonica breeding industry.

The invention relates to the field of biological detection, in particular to a nucleic acid releasing agent and a preparation method and application thereof. The invention provides a nucleic acid releasing agent. The nucleic acid releasing agent comprises chaotropic salt, polyethylene glycol, a surfactant, an antifreeze agent, a penetrant, a metal chelating agent, an antioxidant and a buffer solution. According to the invention, the extraction-free rapid nucleic acid releasing agent is researched and developed, and an ultrasonic treatment scheme is introduced to carry out rapid pretreatment on the sample, so that the problems of tedious nucleic acid extraction process, long time consumption and low efficiency in the prior art are successfully solved, and an efficient, convenient and low-cost nucleic acid releasing and detecting scheme is provided for clinical detection and scientific research.

The invention provides a novel irradiation fresh-keeping method for brain tissues. The novel irradiation fresh-keeping method comprises the following steps: 1) providing irradiation equipment; 2) obtaining animal brain tissues; 3) the animal brain tissue is placed in a radiation source to be irradiated, the irradiation dose is 1 kGy to 100 kGy, and after irradiation, a fixing agent does not need to be used for fixation; (4) dyeing the treated brain tissue; 5) slicing the animal brain tissue; (6) dehydrating and sealing the sliced animal brain tissue; and 7) performing imaging observation on the brain tissue sample. According to the method, a traditional irradiation preservation technology is applied to fixed preservation of the brain tissue for the first time, so that brain tissue degradation and structural damage caused by enzyme in the brain tissue are inhibited. According to the technology, the structure of the brain tissue sample can still be kept complete without adding a chemical reagent, enough fresh brain tissue samples are expected to be provided for brain science research, especially human brain research, and technical support is provided for brain science research.

The invention discloses application of a substance for inhibiting Sqstm1 gene expression and inhibiting Sqstm1 from transmitting phosphoric acid chemical energy to a microtube in an extracellular vesicle secretion inhibitor, and relates to the technical field of biomedicine. The application method comprises the following steps: screening a substance capable of targeting a transcription product of the Sqstm1, an inhibiting substance targeting protein expression of the Sqstm1, and a substance for inhibiting the Sqstm1 from transmitting phosphoric acid chemical energy to a microtubule. The Sqstm1 is used as a key target of the EVs inhibitor as a core, and the main advantage of the Sqstm1 is that the inhibition of the Sqstm1 does not affect the generation of intracellular vesicles. Therefore, the Sqstm1 can be used as a novel vesicle transportation / secretion inhibition target, and a new strategy and thought are provided for scientific research on vesicle transportation / secretion in the future.

To improve process reproducibility and stability, scientific research efficiency, avoid waste of photoresist, and ensure safety of scientific researchers.SOLUTION: A fully automatic photoresist application baking apparatus for scientific researches, comprises: an apparatus main body; a first loading apparatus; a second loading apparatus; a manipulator apparatus; a spin coating apparatus; a baking apparatus; a pipette apparatus; a vision apparatus; and a control apparatus. The manipulator apparatus grips pieces from the first loading apparatus based on positioning recognition of a vision apparatus under a control of the control apparatus. After it is placed in a vacuum suction hole of the spin coating apparatus, the pipette apparatus sucks the photoresist from the second loading apparatus and drips it onto the small pieces. Then the spin coating apparatus performs spin coating processing on the small pieces. The manipulator apparatus grips the small pieces from the spin coating apparatus and places them on the baking apparatus. The baking apparatus performs baking, and then the manipulator apparatus returns the small pieces to the first loading apparatus.SELECTED DRAWING: Figure 2

1. Name of the product in this design: Energy Metabolism Monitor for Rats and Mice. 2. Purpose of this design: For scientific research experiments to detect the physiological metabolism, respiratory movement, etc. of animals. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

1. The name of the design product: upright fluorescent module. 2. The use of the design product: the design product is mainly used in the field of life science research, cell chromosome identification, biomedical observation and other fluorescent substance detection, and can be used with a microscope. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

1. The name of the design product: upright fluorescent module (DD-3). 2. The use of the design product: the design product is mainly used in the fields of life science research, cell chromosome identification, biomedical observation and other fluorescent substance detection, and can be used with a microscope. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

This application proposes a space biological culture device, culture system, and sample addition method, including a first substrate, a second substrate, and a PDMS chip. The PDMS chip is located between the second substrate and the first substrate and is detachably connected to both substrates. The PDMS chip has a stepped structure, including a base and a boss located on the base. A chamber is formed in the PDMS chip, and the projection of the chamber opening is located within the projection of the boss. An inlet and outlet interface communicating with the chamber are provided on the upper surface of the base not covered by the boss, and the chamber opening is not coplanar with the inlet and outlet interfaces. Through structural innovation and modular collaborative design, this application precisely adapts to the special experimental environment of space microgravity, space scarcity, and high-pressure sealing, achieving stability, efficiency, and reliability in space biological culture, and significantly improving the feasibility and data accuracy of space life science research.

The invention discloses a method for detecting unicellular neogenesis RNA (Ribonucleic Acid) and a product thereof. Comprising the following steps: a) performing alkylation modification on 4sU-labeled new RNA by using iodoacetamide to obtain alkylation modified RNA; b) carrying out RNA reverse transcription reaction by using RNA reverse transcriptase by taking the alkylation modified RNA in the step a) as a template to obtain a reverse transcription reaction product; the RNA reverse transcriptase is at least one of the RNA reverse transcriptase of a single-cell RNA library building kit YeaCell < TM >; the step a) and the step b) are completed in the same reaction container. The method is simple and convenient to operate, small in sample loss, high in RNA capture efficiency, low in cross contamination risk, accurate in detection result and high in detection sensitivity, and can be widely applied to scientific research and medical detection.

1. The name of the design product: X-ray biological irradiator (225). 2. The use of the design product: life science research. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view 1.

1.The name of the design product: packaging box (science research material package). 2.The use of the design product: for packaging articles. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best indicates the design points: front view. 5.The design claimed contains color.