831 results about "Peroxidase" patented technology

The invention discloses a copper-based nano-enzyme, a preparation method thereof and application of the copper-based nano-enzyme in organophosphorus pesticide detection, and belongs to the technical field of pesticide residue detection. The copper-based nano-enzyme has a netted-like structure, a high specific surface area and many active sites, the valence state of a copper element is zero-valence Cu, Cu < + > and Cu < 2 + >, the copper-based nano-enzyme has high peroxidase-like activity and laccase-like activity, the detection limit is low, and glyphosate can be sensitively detected; and glyphosate, glufosinate-ammonium and isazofos can be well distinguished from six organophosphorus pesticides with medium concentration, and the method has high selectivity. The nano enzyme provided by the invention is prepared from ammonium citrate and copper nitrate in an N, N-dimethylformamide (DMF) solution by using a solvothermal method; the preparation process of the nano enzyme is simple.

The invention discloses a method for detecting glufosinate-ammonium residues in coffee in dual modes of colorimetry and chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that Schiff base formed by 4-aminoantipyrine and glutaraldehyde serves as a complexing agent of Cu < 2 + >, and then a copper-doped carbon dot Cu-CDs nano-enzyme is prepared through microwave digestion; according to the present invention, the Cu-CDs nano-enzyme has excellent peroxidase-like activity, can oxidize the colorless 2, 2 '-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid into the blue-green oxidized ABTS, and can improve the chemiluminescence intensity of the reaction of N-(4-aminobutyl)-N-ethylisoluminol and H2O2 by nearly 100 times when the Cu-CDs nano-enzyme is used as the chemiluminescence catalyst; based on the inhibition effect of glufosinate-ammonium on the activity of Cu-CDs POD, a sensitive and rapid colorimetric and chemiluminescence two-channel GM detection technology is developed, and the method has potential application value in the aspect of coffee safety monitoring.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

The invention belongs to the technical field of creatine kinase detection kits, and particularly relates to a creatine kinase detection reagent, a creatine kinase detection kit and application, the creatine kinase detection reagent comprises a buffer solution, creatine phosphate, glycerol, glycerol kinase, glycerophosphate oxidase, peroxidase and a color developing agent, and further comprises a CK activation reducing agent and perfluoro and polyfluoroalkyl substances, the CK activation reducing agent is a sulfur-containing reducing agent. According to the method, the detection accuracy can be improved.

The invention discloses a metal-doped carbon quantum dot as well as preparation and application of the metal-doped carbon quantum dot in a nano antibacterial agent, citric acid, urea and tea polyphenol are taken as precursors, metal salt is added, carbon dot synthesis and metal ion doping are synchronously completed in one step through a hydrothermal method, and the metal-doped carbon quantum dot is prepared; the surfaces of the metal-doped carbon quantum dots are negatively charged, and the metal-doped carbon quantum dots have peroxidase-like activity and catalase-like activity at the same time; the metal ions adopt any one of Fe < 3 + >, Cu < 2 + > and Ce < 3 + >; the metal-doped carbon quantum dots are in a sphere-like shape. The metal-doped carbon quantum dots prepared by the preparation method disclosed by the invention have peroxidase-like activity and catalase-like activity at the same time through precise doping of metal ions, and the two activities have a synergistic effect in an oral periodontitis microenvironment, so that a good antibacterial effect is achieved.

The preparation method comprises the following steps: synthesizing gold nanostars (GNS) by using a seed growth method, coating mesoporous cerium dioxide (mCeO2) by using a template method, and loading a photosensitizer indocyanine green (ICG), so as to form a nano enzyme diagnosis and treatment system with chemical activity, photoactivity and photothermal effects. In the system, the gold nanostars have a good photo-thermal effect due to the multi-branched structure of the gold nanostars; on one hand, the mesoporous cerium dioxide coated on the outer layer has a chemical effect due to the nano-enzyme effect, and on the other hand, the mesoporous structure of the mesoporous cerium dioxide can better load the photosensitizer indocyanine green. All the components have good stability and biological safety, also have peroxidase simulation activity and a drug loading function, can improve the level of reactive oxygen species (ROS) in a tumor microenvironment and load photosensitizer drugs, and have an excellent anti-tumor effect and small side effects.

The invention discloses a preparation method of Au (at) Au (at) Ag / Pt / M NPs and application of the Au (at) Au (at) Ag / Pt / M NPs in klebsiella pneumoniae detection, and belongs to the technical field of analysis and detection. The invention particularly relates to development of a signal probe and combination and assembly of the signal probe and lateral flow immunochromatography (LFIA) for klebsiella pneumoniae detection. Au (at) Au (at) Ag / Pt / MNPs is a multi-element noble metal nanoparticle with plasma resonance property, peroxidase-like activity and light stability, shows strong detection potential in three modes of colorimetric (CM), catalytic colorimetric (CL) and surface enhanced Raman scattering (SERS), and is loaded into LFIA to construct a multi-mode LFIA (CM / CL / SERS-LFIA). The kit is expected to be used for direct multimode detection of klebsiella pneumoniae in clinical sputum samples, and a new early detection strategy is provided for respiratory tract infection diseases such as klebsiella pneumoniae.

The present invention relates to novel compounds which are capable of inhibiting semicarbazide-sensitive amine oxidase and myeloperoxidase. These compounds are useful for the treatment of a variety of inflammatory indications, e.g., autoimmune diseases, respiratory disease, fibrosis, vasculitis, neurological diseases or disorders, and the complications resulting from infectious disease, obesity, diabetes / metabolic syndrome and / or cardiovascular disease in human subjects as well as in pets and livestock. In addition, the present invention relates to pharmaceutical compositions containing these compounds, as well as various uses thereof.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention relates to a preparation method and application of a carbon dot-based nano enzyme. The preparation method of the carbon dot-based nano-enzyme comprises the following steps: taking sucralose and B vitamins as raw materials, and preparing the carbon dot-based nano-enzyme through a one-pot hydrothermal method. The preparation method of the carbon dot-based nano-enzyme provided by the invention is simpler in catalytic condition and higher in safety. The carbon dot-based nano-enzyme prepared by the invention has the advantages of high oxidase-like activity, low drug resistance, high biocompatibility and the like, can inhibit multi-drug-resistant bacteria, and can be applied to the aspects of aquatic product preservation and the like.

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses a colorimetric-fluorescent dual-signal salmonella detection method based on Ag-MoS2 nano-enzyme. The method is characterized by comprising the following steps: mixing a single strand S1, a single strand S2, a single strand S3, a single strand S4, a salmonella aptamer and cDNA (complementary deoxyribonucleic acid) to prepare a multivalent aptamer competitive probe solution; respectively dissolving a hairpin probe H1 and a hairpin probe H2 in PBS (Phosphate Buffer Solution); a step of preparing an Ag-MoS2 nano enzyme composite material; the method comprises the following steps: mixing and incubating a to-be-detected salmonella solution and a multivalent aptamer competitive probe, adding the Ag-MoS2 nano-enzyme composite material for performing CHA amplification reaction, then adding an H2O2 solution and a TMB solution for performing peroxidase catalytic reaction, respectively performing colorimetric method and fluorescence method detection, and calculating to obtain the salmonella concentration in the to-be-detected solution. The kit has the advantages of simplicity, rapidness, high sensitivity, strong specificity, high accuracy and real-time sterilization after detection.

The invention discloses peroxidase and application thereof, and relates to the field of bioengineering. The peroxidase POD-8 provided by the invention has very good enzyme activity for oxidizing phenol substrates, especially has the highest enzyme activity for 2, 6-DMP, has very good persistence, and can keep stable enzyme activity within 10 minutes; the resistance to various metal ions is relatively high, and the enzyme activity of 90% or above can still be kept in the presence of various metal ions with various concentrations; the POD-8 has a strong decolorizing capability on a plurality of dyes, especially has a decolorizing efficiency of about 25% on Congo red and amino black 10B in 24 h, and has a good application prospect in the aspect of dye wastewater treatment.

The invention relates to the field of nano-enzymes, in particular to a nano-enzyme, a preparation method and a method for detecting glucose by using the nano-enzyme. The nano-enzyme comprises a carrier and platinum nano-particles coated on the surface of the carrier, and the carrier is oxidized nano-diamond particles with the particle size of 20-30 nm. The core structure of the nano-enzyme, namely a composite system of the carrier and the platinum nano-particles, is defined, the platinum nano-particles serve as a metal nano-material with excellent catalytic performance and are a core component for simulating peroxidase activity, the size of the oxidized nano-diamond carrier is controlled to be 20-30 nm, the dispersity of the material is guaranteed, and the catalytic activity of peroxidase is improved. And the catalytic efficiency of the composite system can be further improved through a size effect. According to the structural design, the problems of insufficient catalytic activity and poor stability of a single nano material are solved through the synergistic effect of the carrier and the load component, and a material foundation is laid for subsequent efficient glucose detection.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention provides a microneedle preparation for co-delivering photo-thermal nano-enzyme and elemene as well as a preparation method and application of the microneedle preparation. The preparation method comprises the following steps: firstly, preparing a novel photo-thermal nano enzyme Au (at) MoS2 through a two-step water phase synthesis strategy; benefited from the synergistic effect of the hybrid material, the nano-enzyme shows significantly enhanced near-infrared (NIR) photothermal effect, peroxidase-like (POD) activity and glutathione-like peroxidase (GSHOx) activity, and can effectively remodel the tumor microenvironment and destroy the redox steady state. Furthermore, photo-thermal nano enzyme, beta-ELE and hyaluronic acid are used as raw materials, and a novel soluble microneedle preparation is successfully prepared through a template method. The novel composite microneedle is in a sharp rectangular pyramid shape, growth of melanoma can be effectively inhibited through percutaneous delivery of photo-thermal nano-enzyme and beta-ELE, remarkable systemic toxicity is avoided, and an innovative strategy is provided for non-invasive, efficient and safe melanoma combined treatment.

The invention relates to the technical field of biological materials, in particular to silver nano-chlorogenic acid composite hydrogel as well as preparation and application thereof. According to the invention, the CGA and the silver nitrate are used for green synthesis of the nano-particles CGA-AgNPs, and the nano-particles have peroxide nano-enzyme activity and antibacterial activity; and the CGA-AgNPs and chitosan (CS) are cross-linked to form the silver nano-chlorogenic acid composite hydrogel CGA-AgNPs-CS, so that the uniform distribution of nano-particles in the hydrogel can be promoted. According to the invention, multi-functionalization is taken as a target, a novel hydrogel composition system is designed from the source, the provided preparation method is green, simple, rapid and environment-friendly, the obtained composite hydrogel has multiple functions, and a good foundation is laid for expansion and application of the composite hydrogel in the biomedical fields of wound repair, wearable equipment, nano antibacterial drugs and the like.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses a method for detecting acrylamide in coffee based on colorimetric and photo-thermal dual modes of MXene / MOFs nano-enzyme, and belongs to the technical field of chemical analysis and detection.According to the method, LiF and HCl are used for etching Ti3AlC2 to obtain Mxene, a solvothermal method is adopted for synthesizing Mn / Fe-MIL-NH2, Mxene coated Mn / Fe-MIL-NH2 nano-enzyme is obtained in a self-assembly mode, the Mxene coated Mn / Fe-MIL-NH2 nano-enzyme has peroxidase-like activity and photo-thermal performance, and the Mxene coated Mn / Fe-MIL-NH2 nano-enzyme can be used for detecting acrylamide in coffee in the colorimetric and photo-thermal dual modes. Under irradiation of near-infrared light 808 nm, peroxidase-like activity of the Mxene-coated Mn / Fe-MIL-NH2 nano-enzyme is improved, due to addition of acrylamide, the speed of oxidizing a substrate TMB by the Mxene-coated Mn / Fe-MIL-NH2 nano-enzyme is increased, meanwhile, the temperature of the oxidized TMB with photosensitive performance is also increased, and the method has the advantages of being high in sensitivity, high in specificity, easy to operate, rapid and the like.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention relates to the technical field of mineral materials and nano-enzyme catalysis, in particular to an iron-containing layered silicate mineral nano-enzyme as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out ball milling on the iron-containing layered silicate mineral, carrying out centrifugal washing, and drying to obtain the iron-containing layered silicate mineral nano-enzyme. According to the method, silicate minerals with a layered structure and rich iron content are adopted as raw materials, the ball-milled layered silicate mineral nano-enzyme is successfully prepared through a ball milling method, the crystallinity of the minerals is reduced, the surface acidity of the minerals is improved, and low-spin iron is generated, so that the affinity of the ball-milled minerals to H2O2 is enhanced; h2O2 is catalyzed to generate singlet oxygen and superoxide free radicals, so that the peroxidase-like activity of the ball-milled minerals is improved. The enzyme-like activity is improved by 10 times, high catalytic activity can still be kept under the neutral condition, and the method can be used for colorimetric sensing and antibacterial application of small biological molecules such as glucose, hydrogen peroxide and glutathione.

The invention relates to a screening method and an activity evaluation method of a natural antioxidant, and the screening method is characterized in that a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant reaction system is constructed, and the activity of the natural antioxidant is evaluated through a high performance liquid chromatography-ultraviolet detector-tandem mass spectrometry analysis technology. Screening of the natural antioxidant is realized; according to the activity evaluation method, a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant + TMB reaction system is constructed, and the activity evaluation of the natural antioxidant is realized through an oxidized TMB photo-thermal detection technology. According to the method disclosed by the invention, the used reaction system is high in applicability, high in accuracy and good in selectivity.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

Provided herein are methods for producing soybean plants having high protein using marker-assisted selection. The disclosure further provides methods for introgressing one or more loci comprising at least a high-protein allele linked to the high-protein QTL, thus producing high-protein soybean plants. Methods of increasing protein content in soybean plants and plant parts by decreasing the activity of a peroxidase gene are provided herein.

The invention discloses a glucose-responsive hydrogen sulfide release cascade nano-enzyme as well as a preparation method and application thereof. The cascaded nano-enzyme takes BSA as a growth template and a carrier, and FeS and Au nano-particles are loaded. The FeS-coated Au is synthesized by adopting a biomineralization method, so that the co-loading of FeS and Au is realized, and good biocompatibility is achieved. Meanwhile, by combining the peroxidase-like activity of FeS and the glucose oxidase-like activity of Au, glucose can be catalyzed in a cascade manner to generate toxic hydroxyl free radicals, and drug-resistant bacteria are efficiently killed. In addition, the FeS (at) Au cascade nano-enzyme has a hydrogen sulfide gas release function, and can promote vascular regeneration by up-regulating a hypoxia inducible factor-1 pathway. The cascade nano-enzyme responding to the hyperglycemia microenvironment is combined with a gas treatment mechanism, blood glucose can be effectively consumed, drug-resistant bacterium infection can be eliminated, revascularization can be promoted, and a new strategy is provided for treatment of diabetes wounds infected by drug-resistant bacteria and regeneration of skin tissues.

A test strip for detecting uric acid in saliva that meets the requirement for room-temperature preservation, a preparation and detection method therefor and the use thereof. The test strip for detecting uric acid in saliva comprises a uricase, a peroxidase, a vitamin C oxidase, a bilirubin oxidase, a color developing agent, and a protective film coated on the test strip for detecting uric acid in saliva. To meet the needs of a target population for long-term monitoring and real-time tracking of changes in their uric acid levels, by means of agent addition, the test strip for detecting uric acid in saliva has prolonged preservation time at room temperature, enables non-invasive, efficient and convenient monitoring of a health status at any time, and timely detection and treatment, allows intuitive visual identification of uric acid levels in saliva by a subject, and meets the needs of a subject for long-term preservation at room temperature.

The invention discloses a method for detecting glucose on the basis of cascade catalysis of cerium dioxide / gold nano cluster-Prussian blue nano enzyme (AuNCs-CeO-HMPB). The material is prepared by compounding cerium dioxide (CeO) with similar glucose oxidase activity and hollow Prussian blue nanoparticles (AuNCs-HMPB) modified by gold nanoclusters with similar peroxidase activity through a hydrothermal assembly method. In the presence of glucose, CeO catalyzes oxidation of glucose to generate gluconic acid and H2O2, then AuNCs-HMPB synergistically catalyzes decomposition of newly generated H2O2 to generate high active oxygen species, further a colorless 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) color developing agent is oxidized to generate obvious color change, and based on the cascade catalytic reaction, the enzyme-free glucose colorimetric sensor is successfully constructed. The sensor shows excellent detection performance on glucose under optimized conditions (pH = 4.0, 50 DEG C), the linear detection range is 10.00-90.00 [mu] mol / L, and the detection limit is 2.1 [mu] mol / L (S / N = 3).

The invention provides a cordyceps militaris fermentation extract and application thereof in preservation of agaricus bisporus, and belongs to the technical field of deep processing of edible mushrooms and preservation of agricultural products. The preparation method of the cordyceps militaris fermentation extract comprises the following steps: (1) taking cordyceps militaris sporocarp, adding water, grinding, and performing enzymolysis by using cellulase and papain in sequence to obtain cordyceps militaris pulp; (2) adding glucose and waste beer yeast powder into the cordyceps militaris pulp, then inoculating lactobacillus paracasei and bifidobacterium bifidum, and fermenting; and (3) after fermentation is completed, carrying out ultrasonic crushing, centrifuging to remove thalli, and carrying out spray drying to obtain the cordyceps militaris fermentation extract. The method can effectively reduce the browning index and weight loss ratio of agaricus bisporus, can slow down the increase of malonaldehyde, reduces the activity of PPO enzyme, and prevents enzymatic browning caused by the synergistic effect of the PPO enzyme and peroxidase. The SOD enzyme activity in the agaricus bisporus can be kept, active oxygen in tissues can be removed, and the metabolic balance of the active oxygen can be maintained.

The invention discloses a preparation method and application of chalcogenide doped graphdiyne, chalcogenide is implanted into a chalcogenide cavity in a simple thermal driving mode by using a chalcogenide cavity formed in para-nitrogen substituted graphdiyne as an anchoring site, so that the chalcogenide is distributed in a lattice shape, and a high-load monatomic graphdiyne material is obtained. The nano-enzyme sensor is constructed by utilizing the activity of peroxidase-like enhanced by the chalcogenide doped graphdiyne to detect bisphenol and thiosulfate ions, and powerful support is provided for monitoring of food safety and environmental safety.