1139 results about "Peroxidase" patented technology

The invention relates to the technical field of optical sensing, in particular to a copper-carbon quantum dot nano-enzyme and a preparation method and application thereof. Poria cocos and Cu < 2 + > are taken as raw materials, and the copper-carbon quantum dots with excellent peroxidase activity are prepared by adopting a one-pot hydrothermal method and can be used for sensitively, accurately and selectively detecting glyphosate. Cu < 2 + > is combined with oxygen-rich and nitrogen-rich components in poria cocos to generate an oxidation-reduction reaction, CuCDs with Cu < 2 + > / Cu < + > are obtained, Cu < 2 + > doping changes electron cloud distribution of the CDs, the electron transmission capability of the CuCDs is enhanced, and Cu < 2 + > and the CDs have a synergistic effect to generate three ROS (. OH,. O2 <-> and < 1 > O2), so that excellent peroxidase activity is exerted. The glyphosate is chelated with hydroxyl, amino, carbonyl, carboxyl and Cu on the surface of the CuCDs to occupy active sites, so that the active sites are poisoned, the peroxidase activity of the CuCDs is selectively inhibited, and a powerful choice is provided for simply, quickly, sensitively and selectively detecting the glyphosate.

The invention relates to peroxidase AniUPO or a mutant of the peroxidase AniUPO, application of the peroxidase AniUPO in synthesis of alpha-hydroxyl beta-ketoester derivatives and a method for synthesizing the alpha-hydroxyl beta-ketoester derivatives, and belongs to the field of enzyme engineering. The invention provides a peroxidase AniUPO mutant, which is an amino acid sequence obtained by taking wild type peroxidase AniUPO with an amino acid sequence as shown in SEQ ID NO.1 as a parent and mutating the wild type peroxidase AniUPO with the amino acid sequence as shown in SEQ ID NO.1. The invention also provides a DNA (Deoxyribonucleic Acid) molecule for coding the peroxidase AniUPO mutant, an expression vector, a host cell and a method for synthesizing the chiral alpha-hydroxyl beta-ketone ester derivative. Compared with a wild type, the peroxidase mutant has the advantages of higher catalytic activity and better selectivity, applicable substrate types can be expanded to various cyclic and non-cyclic beta-ketone ester substrates, expensive catalysts and ligands are not needed, reaction conditions are mild, and potential industrial application prospects are achieved.

The invention discloses a preparation method and application of a zinc-manganese bimetal organic framework derived composite nano-enzyme, and belongs to the technical field of nano-materials. Zinc-manganese bimetal organic framework nanoparticles prepared by reacting ZIF-8 with manganese (III) acetylacetonate are used as a template, and are calcined at different temperatures in a tubular furnace to obtain zinc-manganese bimetal organic framework derivative composite nano-enzymes with different morphologies. The nano enzyme has peroxidase-like activity, glutathione peroxidase-like activity and catalase-like activity, and has a wide application prospect in anti-tumor treatment.

The invention discloses a copper-based nano-enzyme, a preparation method thereof and application of the copper-based nano-enzyme in organophosphorus pesticide detection, and belongs to the technical field of pesticide residue detection. The copper-based nano-enzyme has a netted-like structure, a high specific surface area and many active sites, the valence state of a copper element is zero-valence Cu, Cu < + > and Cu < 2 + >, the copper-based nano-enzyme has high peroxidase-like activity and laccase-like activity, the detection limit is low, and glyphosate can be sensitively detected; and glyphosate, glufosinate-ammonium and isazofos can be well distinguished from six organophosphorus pesticides with medium concentration, and the method has high selectivity. The nano enzyme provided by the invention is prepared from ammonium citrate and copper nitrate in an N, N-dimethylformamide (DMF) solution by using a solvothermal method; the preparation process of the nano enzyme is simple.

The invention discloses a method for detecting glufosinate-ammonium residues in coffee in dual modes of colorimetry and chemiluminescence, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that Schiff base formed by 4-aminoantipyrine and glutaraldehyde serves as a complexing agent of Cu < 2 + >, and then a copper-doped carbon dot Cu-CDs nano-enzyme is prepared through microwave digestion; according to the present invention, the Cu-CDs nano-enzyme has excellent peroxidase-like activity, can oxidize the colorless 2, 2 '-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid into the blue-green oxidized ABTS, and can improve the chemiluminescence intensity of the reaction of N-(4-aminobutyl)-N-ethylisoluminol and H2O2 by nearly 100 times when the Cu-CDs nano-enzyme is used as the chemiluminescence catalyst; based on the inhibition effect of glufosinate-ammonium on the activity of Cu-CDs POD, a sensitive and rapid colorimetric and chemiluminescence two-channel GM detection technology is developed, and the method has potential application value in the aspect of coffee safety monitoring.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

The invention belongs to the technical field of creatine kinase detection kits, and particularly relates to a creatine kinase detection reagent, a creatine kinase detection kit and application, the creatine kinase detection reagent comprises a buffer solution, creatine phosphate, glycerol, glycerol kinase, glycerophosphate oxidase, peroxidase and a color developing agent, and further comprises a CK activation reducing agent and perfluoro and polyfluoroalkyl substances, the CK activation reducing agent is a sulfur-containing reducing agent. According to the method, the detection accuracy can be improved.

The invention discloses a metal-doped carbon quantum dot as well as preparation and application of the metal-doped carbon quantum dot in a nano antibacterial agent, citric acid, urea and tea polyphenol are taken as precursors, metal salt is added, carbon dot synthesis and metal ion doping are synchronously completed in one step through a hydrothermal method, and the metal-doped carbon quantum dot is prepared; the surfaces of the metal-doped carbon quantum dots are negatively charged, and the metal-doped carbon quantum dots have peroxidase-like activity and catalase-like activity at the same time; the metal ions adopt any one of Fe < 3 + >, Cu < 2 + > and Ce < 3 + >; the metal-doped carbon quantum dots are in a sphere-like shape. The metal-doped carbon quantum dots prepared by the preparation method disclosed by the invention have peroxidase-like activity and catalase-like activity at the same time through precise doping of metal ions, and the two activities have a synergistic effect in an oral periodontitis microenvironment, so that a good antibacterial effect is achieved.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention relates to a preparation method and application of an electrochemical-colorimetric dual-mode sensor based on nanoflowers and a bifunctional NiFe-PBA nano enzyme. The NiFe-PBA loaded gold-platinum bimetallic nanoparticles have excellent oxidation-reduction activity and peroxidase activity, and are used as a bifunctional probe to construct an electrochemical colorimetric dual-mode sensor. More signal probes are introduced into the branched DNA structure to participate in the reaction, the specificity of the sensor is enhanced through specific recognition of the aptamer on OTA, and finally, an electrochemical and colorimetric dual-mode channel is ingeniously constructed by utilizing a magnetic separation technology, so that environmental interference is effectively reduced, and false positive signals are avoided. NiFe-PBA loaded gold-platinum bimetallic nanoparticles participate in the dual-mode channels, so that the experimental operation is simplified while the signal response caused by the target is realized. The dual-mode sensor constructed by the invention has the characteristics of sensitivity, reliability, rapidness and stability, and is beneficial to popularization and application. And the biosensor has good anti-interference capability and practical application capability, and provides a novel biosensing platform for practical sample analysis.

The preparation method comprises the following steps: synthesizing gold nanostars (GNS) by using a seed growth method, coating mesoporous cerium dioxide (mCeO2) by using a template method, and loading a photosensitizer indocyanine green (ICG), so as to form a nano enzyme diagnosis and treatment system with chemical activity, photoactivity and photothermal effects. In the system, the gold nanostars have a good photo-thermal effect due to the multi-branched structure of the gold nanostars; on one hand, the mesoporous cerium dioxide coated on the outer layer has a chemical effect due to the nano-enzyme effect, and on the other hand, the mesoporous structure of the mesoporous cerium dioxide can better load the photosensitizer indocyanine green. All the components have good stability and biological safety, also have peroxidase simulation activity and a drug loading function, can improve the level of reactive oxygen species (ROS) in a tumor microenvironment and load photosensitizer drugs, and have an excellent anti-tumor effect and small side effects.

The invention discloses a preparation method of Au (at) Au (at) Ag / Pt / M NPs and application of the Au (at) Au (at) Ag / Pt / M NPs in klebsiella pneumoniae detection, and belongs to the technical field of analysis and detection. The invention particularly relates to development of a signal probe and combination and assembly of the signal probe and lateral flow immunochromatography (LFIA) for klebsiella pneumoniae detection. Au (at) Au (at) Ag / Pt / MNPs is a multi-element noble metal nanoparticle with plasma resonance property, peroxidase-like activity and light stability, shows strong detection potential in three modes of colorimetric (CM), catalytic colorimetric (CL) and surface enhanced Raman scattering (SERS), and is loaded into LFIA to construct a multi-mode LFIA (CM / CL / SERS-LFIA). The kit is expected to be used for direct multimode detection of klebsiella pneumoniae in clinical sputum samples, and a new early detection strategy is provided for respiratory tract infection diseases such as klebsiella pneumoniae.

The invention relates to the technical field of nano-drugs, in particular to an acoustic / chemical power synergistic anti-tumor nano-drug as well as a preparation method and application thereof. The invention provides an acoustic / chemical power synergistic anti-tumor nano-drug, which takes hollow mesoporous MnO2 nano-particles as a carrier, and is loaded with vitamin C and a sound-sensitive agent chlorin e6. Vc can generate abundant H2O2 to provide a raw material for CDT, and MnO2 is used as a peroxidase-like enzyme to catalyze H2O2 to generate O2, so that tumor hypoxia is effectively relieved, and the effect of sonodynamic therapy is enhanced. Besides, GSH induces MnO2 degradation, GSH consumption is caused to enhance CDT, GSH depletion can cause glutathione peroxidase 4 (GPX4) activity reduction, ROS generation and GPX4 activity reduction have a synergistic effect, lipid peroxides (LPOs) are increased, and finally ferroptosis of tumor cells is caused.

The present invention relates to novel compounds which are capable of inhibiting semicarbazide-sensitive amine oxidase and myeloperoxidase. These compounds are useful for the treatment of a variety of inflammatory indications, e.g., autoimmune diseases, respiratory disease, fibrosis, vasculitis, neurological diseases or disorders, and the complications resulting from infectious disease, obesity, diabetes / metabolic syndrome and / or cardiovascular disease in human subjects as well as in pets and livestock. In addition, the present invention relates to pharmaceutical compositions containing these compounds, as well as various uses thereof.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention relates to a preparation method and application of a carbon dot-based nano enzyme. The preparation method of the carbon dot-based nano-enzyme comprises the following steps: taking sucralose and B vitamins as raw materials, and preparing the carbon dot-based nano-enzyme through a one-pot hydrothermal method. The preparation method of the carbon dot-based nano-enzyme provided by the invention is simpler in catalytic condition and higher in safety. The carbon dot-based nano-enzyme prepared by the invention has the advantages of high oxidase-like activity, low drug resistance, high biocompatibility and the like, can inhibit multi-drug-resistant bacteria, and can be applied to the aspects of aquatic product preservation and the like.

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses a colorimetric-fluorescent dual-signal salmonella detection method based on Ag-MoS2 nano-enzyme. The method is characterized by comprising the following steps: mixing a single strand S1, a single strand S2, a single strand S3, a single strand S4, a salmonella aptamer and cDNA (complementary deoxyribonucleic acid) to prepare a multivalent aptamer competitive probe solution; respectively dissolving a hairpin probe H1 and a hairpin probe H2 in PBS (Phosphate Buffer Solution); a step of preparing an Ag-MoS2 nano enzyme composite material; the method comprises the following steps: mixing and incubating a to-be-detected salmonella solution and a multivalent aptamer competitive probe, adding the Ag-MoS2 nano-enzyme composite material for performing CHA amplification reaction, then adding an H2O2 solution and a TMB solution for performing peroxidase catalytic reaction, respectively performing colorimetric method and fluorescence method detection, and calculating to obtain the salmonella concentration in the to-be-detected solution. The kit has the advantages of simplicity, rapidness, high sensitivity, strong specificity, high accuracy and real-time sterilization after detection.

The invention discloses peroxidase and application thereof, and relates to the field of bioengineering. The peroxidase POD-8 provided by the invention has very good enzyme activity for oxidizing phenol substrates, especially has the highest enzyme activity for 2, 6-DMP, has very good persistence, and can keep stable enzyme activity within 10 minutes; the resistance to various metal ions is relatively high, and the enzyme activity of 90% or above can still be kept in the presence of various metal ions with various concentrations; the POD-8 has a strong decolorizing capability on a plurality of dyes, especially has a decolorizing efficiency of about 25% on Congo red and amino black 10B in 24 h, and has a good application prospect in the aspect of dye wastewater treatment.

The invention relates to the field of nano-enzymes, in particular to a nano-enzyme, a preparation method and a method for detecting glucose by using the nano-enzyme. The nano-enzyme comprises a carrier and platinum nano-particles coated on the surface of the carrier, and the carrier is oxidized nano-diamond particles with the particle size of 20-30 nm. The core structure of the nano-enzyme, namely a composite system of the carrier and the platinum nano-particles, is defined, the platinum nano-particles serve as a metal nano-material with excellent catalytic performance and are a core component for simulating peroxidase activity, the size of the oxidized nano-diamond carrier is controlled to be 20-30 nm, the dispersity of the material is guaranteed, and the catalytic activity of peroxidase is improved. And the catalytic efficiency of the composite system can be further improved through a size effect. According to the structural design, the problems of insufficient catalytic activity and poor stability of a single nano material are solved through the synergistic effect of the carrier and the load component, and a material foundation is laid for subsequent efficient glucose detection.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention provides a microneedle preparation for co-delivering photo-thermal nano-enzyme and elemene as well as a preparation method and application of the microneedle preparation. The preparation method comprises the following steps: firstly, preparing a novel photo-thermal nano enzyme Au (at) MoS2 through a two-step water phase synthesis strategy; benefited from the synergistic effect of the hybrid material, the nano-enzyme shows significantly enhanced near-infrared (NIR) photothermal effect, peroxidase-like (POD) activity and glutathione-like peroxidase (GSHOx) activity, and can effectively remodel the tumor microenvironment and destroy the redox steady state. Furthermore, photo-thermal nano enzyme, beta-ELE and hyaluronic acid are used as raw materials, and a novel soluble microneedle preparation is successfully prepared through a template method. The novel composite microneedle is in a sharp rectangular pyramid shape, growth of melanoma can be effectively inhibited through percutaneous delivery of photo-thermal nano-enzyme and beta-ELE, remarkable systemic toxicity is avoided, and an innovative strategy is provided for non-invasive, efficient and safe melanoma combined treatment.

The invention relates to the technical field of biological materials, in particular to silver nano-chlorogenic acid composite hydrogel as well as preparation and application thereof. According to the invention, the CGA and the silver nitrate are used for green synthesis of the nano-particles CGA-AgNPs, and the nano-particles have peroxide nano-enzyme activity and antibacterial activity; and the CGA-AgNPs and chitosan (CS) are cross-linked to form the silver nano-chlorogenic acid composite hydrogel CGA-AgNPs-CS, so that the uniform distribution of nano-particles in the hydrogel can be promoted. According to the invention, multi-functionalization is taken as a target, a novel hydrogel composition system is designed from the source, the provided preparation method is green, simple, rapid and environment-friendly, the obtained composite hydrogel has multiple functions, and a good foundation is laid for expansion and application of the composite hydrogel in the biomedical fields of wound repair, wearable equipment, nano antibacterial drugs and the like.

The invention belongs to the technical field of medicines, and particularly relates to a bionic nano-drug for resisting subcutaneous drug-resistant bacterial infection as well as a preparation method and application thereof. Dopamine hydrochloride is added in the synthesis process of a metal-organic framework to prepare a dandelion-shaped carbon nano-enzyme precursor, then the nano-enzyme precursor is carbonized to obtain a carbon nano-enzyme with targeting property, photoresponse and peroxidase activity, and finally berberine hydrochloride is loaded on the carbon nano-enzyme to obtain the bionic nano-drug. The bionic nano-drug provided by the invention firstly has good biocompatibility, secondly has a unique dandelion-shaped form, is beneficial to playing a targeting role, and finally has relatively strong light response capability in a near-infrared region, and can synergistically and efficiently sterilize through action mechanisms of antibacterial drugs, photothermal therapy and chemical kinetics, so that the bionic nano-drug has a good application prospect. The compound has an important application value in preparation of drugs for treating subcutaneous drug-resistant bacterium infection.

The invention provides a preparation method of Keemun camellia seed fermented oil, and belongs to the technical field of vegetable oil production. The preparation method comprises the following steps: S1, preparing recombinant saccharomycetes; s2, jointly fermenting the antarctic torulopsis, the recombinant saccharomycetes and the Keemun camellia seed oil; s3, separating an oil phase from a water phase, and taking the oil phase; and S4, dehydrating the oil phase to obtain the Keemun camellia seed fermented oil. Preferably, according to the preparation method of the recombinant saccharomycetes in the step S1, phenylalanine deaminase, cinnamate hydroxylase, 4-coumaric acid CoA ligase, chalcone synthetase, chalcone reductase and peroxidase are expressed in saccharomyces cerevisiae. According to the Keemun camellia seed fermented oil prepared by the method disclosed by the invention, the characteristic components of the Keemun camellia seed oil can be remarkably improved, the antioxidant capacity of the Keemun camellia seed oil is enhanced while the skin feeling of the Keemun camellia seed oil is improved, and the application value of the Keemun camellia seed oil in cosmetics is improved.

The invention discloses a Vinck Cellulomicrobe and application thereof in degradation of corn straw and improvement of soil fertility, and belongs to the field of agricultural microbial technology and solid waste resource utilization. The bacterial strain can secrete a plurality of hydrolase and oxidase including AA10 family lytic polysaccharide monooxygenase, AA2 peroxidase, GH3 and GH1 beta-glucosidase, GH16 / GH17 beta-glucosan endonuclease and AA1 multi-copper oxidase. The bacterial strain has the advantages that the bacterial strain can secrete a plurality of hydrolase and oxidase; fermentation liquor is applied to soil according to the straw mass ratio of 1: 5, and the corn straw degradation rate can be increased under the condition that the mass ratio of soil to straw is 100: 1. Meanwhile, the microbial agent can synergistically improve soil nutrients and increase the contents of rapidly available phosphorus, rapidly available potassium, alkali-hydrolyzable nitrogen, soluble organic carbon and total phosphorus in soil. According to the microbial agent, a straw lignocellulose structure is effectively cracked through a multi-enzyme system synergistic effect, and the dual effects of accelerating straw degradation and improving soil fertility are achieved.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention discloses a method for detecting acrylamide in coffee based on colorimetric and photo-thermal dual modes of MXene / MOFs nano-enzyme, and belongs to the technical field of chemical analysis and detection.According to the method, LiF and HCl are used for etching Ti3AlC2 to obtain Mxene, a solvothermal method is adopted for synthesizing Mn / Fe-MIL-NH2, Mxene coated Mn / Fe-MIL-NH2 nano-enzyme is obtained in a self-assembly mode, the Mxene coated Mn / Fe-MIL-NH2 nano-enzyme has peroxidase-like activity and photo-thermal performance, and the Mxene coated Mn / Fe-MIL-NH2 nano-enzyme can be used for detecting acrylamide in coffee in the colorimetric and photo-thermal dual modes. Under irradiation of near-infrared light 808 nm, peroxidase-like activity of the Mxene-coated Mn / Fe-MIL-NH2 nano-enzyme is improved, due to addition of acrylamide, the speed of oxidizing a substrate TMB by the Mxene-coated Mn / Fe-MIL-NH2 nano-enzyme is increased, meanwhile, the temperature of the oxidized TMB with photosensitive performance is also increased, and the method has the advantages of being high in sensitivity, high in specificity, easy to operate, rapid and the like.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention relates to the technical field of preparation and application of functional materials, in particular to a fullerene / metal organic framework loaded with acetylcholin esterase as well as a preparation method and application of the fullerene / metal organic framework. The preparation method comprises the following steps: firstly, preparing a fullerene / metal organic framework C60-coated PCN-222 (Fe) nano-enzyme with a space limited environment, and providing a self-cascade amplification strategy without external energy input based on the activities of oxidase-like OXD and peroxidase-like POD of the fullerene / metal organic framework C60-coated PCN-222 (Fe) nano-enzyme; then, the C60 atched PCN-222 (Fe) is combined with acetylcholin esterase AChE by means of molecular interaction, and an AChE / C60 atched PCN-222 (Fe) enzyme-nano enzyme array flow reactor is further developed. The sensor realizes parallel real-time detection and distinguishing of a plurality of organophosphorus pesticides, and has the advantages of wide linear range, high sensitivity and low detection limit. A promising strategy is provided for design of a nano-enzyme and trace pollutant detection system, and a sensing platform is stable in performance, low in price and convenient for commercialized application.