432results about How to "Not easy to miss" patented technology

The invention discloses a method for constructing a Glrx1 gene knock-out animal model based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas9 technology. The method comprises the following steps: 1, selecting and designing gRNA of Glrx1 genes of targeted mice; 2, constructing an sgRNA vector; 3, performing in vitro transcription on sgRNA; 4, injecting one cell stage fertilized eggs of mice; 5, birth and identification of F0-generation mice; and 6, reproducing positive F0-generation mice, and realizing birth and identification of F1-generation mice. According to the method disclosed by the invention, the Glrx1 gene knock-out animal model can be successfully obtained.

Disclosed is a lane level navigation method. High-precision positioning is performed on a vehicle and a road segment where the vehicle is located, and the high-precision positioning accuracy is not lower than the centimeter level. The method comprises the following steps that S20, a navigation path is planned for the vehicle according to road information and road condition information; S21, whether the distance between the vehicle and the nearest road intersection in the driving direction of the navigation path is smaller than or equal to a preset value or not is monitored in real time, if yes, the step S22 is executed; if not, the step S21 is executed continuously; S22, lane matching is performed; S23, whether the vehicle is located on the correct lane or not is judged, and the correct lane is a driving lane planned by a navigation center on the navigation path; if yes, the step S21 is executed again; if not, the step S24 is executed; S24, the path is planned to guide the vehicle into the correct lane, and after the vehicle passes through the road intersection, the step S21 is executed again. The lane level positioning method and system have the advantages that the positioning precision is high, the lane where the vehicle is located can be obtained in real time, the vehicle is planned and guided in real time to enter the correct lane, and the vehicle is not likely to miss the intersection where the vehicle needs to steer.

The invention relates to a pressure pipeline ultrasonic internal detection automation device; a purpose comprises that the provided device can meet detection requirements of a larger-diameter pipeline and has the characteristics of high detection accuracy and high detection efficiency. The technical scheme comprises that the pressure pipeline ultrasonic automatic detection device comprises a pipeline crawling robot, a probe assembly device, encoders, centralizers, supporting frames, a phased array module device and an ultrasonic detecting instrument; the probe assembly device comprises a first annular phased array probe assembly, a second annular phased array probe assembly, a conical phased array probe assembly for detecting defects of inner and outer surfaces and an interior circumference, and corresponding probe holding devices; the first annular phased array probe assembly, the second annular phased array probe assembly and the conical phased array probe assembly are each composed of more than 3 of 128 array element phased array probes; ultrasonic waves emitted by piezoelectric crystal plates of the probe assemblies firstly pass through an annular concave lens and then scan circumferential pipeline walls.

The invention discloses an intelligent electronic medicine box and a control method thereof. The intelligent electronic medicine box comprises a cylindrical box body. The side wall of the box body is provided with a medicine exit which is covered with a chamber cover. The box body is internally provided at least three medicine storage chambers arranged in the circumferential direction. The medicine storage chambers may rotate with a spindle. The box body is further provided with a medicine entry which is communicated with every medicine storage chamber and which is covered with an entry cover. The box body is also internally provided with a rotating motor and a control unit. An output shaft of the rotating motor is connected with the spindle. The control unit controls the rotating motor to rotate according to a preset medication time. The intelligent electronic medicine box has the significant advantages that the box is simple to operate and easy to use and effectively helps family members of a patient take care of the poorly self-sufficient patient and old people by using the mobile internet technology.

The invention discloses a video retrieval method and system. The method includes the steps that videos captured by a video capturing device are stored; a retrieval command input by a user is received, wherein the retrieval command carries a target event; an image containing the target event is retrieved from the stored videos. According to a certain monitoring task such as human face recognition, vehicle license plate recognition and intrusion detection, surveillance video images do not need to be checked manually, and only the retrieval command carrying the target event and corresponding to the monitoring task needs to be input in the system, so that the image containing the target event is retrieved from the stored videos by the system. According to the video retrieval method and system, the condition that part of video images are prone to be missed in manual monitoring is avoided to a certain degree, effective utilization of the captured monitoring videos can be improved, workers are assisted in completing the monitoring task effectively, and the operation efficiency of a security and protection service is further improved.

The invention discloses a method for detecting bridge from remote sensing images. Considering the position relation between bridge and river, firstly, segment a river region using the two steps of coarse segmentation and fine segmentation according to the properties of the water area in the remote sensing image; next, execute a Beamlet transformation on the river region, and accurately detect and segment the bridge according to the properties of the bridge by using the obtained line characteristic information on the basis of the Beamlet transformation, and considering the long straight-line feature of the bridge, implement a post-processing on the obtained result to achieve the final result of bridge detection. Because the Beamlet transformation is used to extract the line characteristic information of the bridge to be detected, the anti-noise performance is enhanced by means of the anti-noise property of the Beamlet transformation; by using the extracted line characteristic information from the Beamlet transformation, the bridge is detected, and compared with the prior technique of using the method of searching bridge points, the invention has no limitation on the width of the bridge, the detection rate is beyond 86 percent, and missed detection and false detection are not easy to occur.

The invention discloses a shoemaking device.The shoemaking device comprises a drying device, a cooling device and a delasting mechanism.The shoemaking device further comprises a conveying mechanism, a press fitting mechanism, an induction unit and a control unit.The conveying mechanism is arranged on the drying device, the press fitting mechanism, the cooling device and the delasting mechanism in a penetrating mode to define an annular conveying mechanism.The conveying mechanism comprises an upper track, a lower track and a driving motor used for driving the upper track and the lower track to generate horizontal annular displacement, wherein the upper track is used for carrying soles, and supports used for positioning and supporting lasts are formed on the lower track.The press fitting mechanism is arranged between the drying device and the cooling device and comprises pressure bearing tables, auxiliary clamping components, driving air cylinders and pressure applying units, wherein the pressure bearing tables are arranged on one or two sides of the conveying mechanism and used for supporting the lasts.According to the shoemaking device, various devices needed in the shoemaking process are combined into a whole, automatic operation is achieved under driving of the control unit, and thus manual cost is substantially lowered.

This invention discloses a ultrasonic wave detector and detection method thereof, which contains ultrasonic wave probe and linkage device, transmitting / receiving circuit, control / process unit, display device and man-machine conversion interface, wherein the ultrasonic wave probe and linkage device having probe position equal displacement moving signal generator composed of mechanical counter or photoelectric mouse structure synchronous moved with probe, generating pulse displacement mark signal of horizontal scale and / or ordinate scale or grid mark signal, synchronous control transmission circuit generating high voltage excitation pulse to probe which generating ultrasonic wave and transmitting to tested object by coupling. Said invention overcomes the image distortion due to non uniform speed movement.

The invention relates to a region traversing method and chip of a robot and a cleaning robot. The method and the chip can improve the cleaning quality of the robot. The region traversing method of therobot comprises the steps that firstly a cleanable range of a grid area is delineated, then planed cleaning is performed in the delineated area, the robot can know the approximate terrain in advanceduring cleaning and can makes a plan in advance, thereby improving the cleaning quality ad efficiency of the robot. For example, it can be known that the robot is ready to reach a physical boundary only through a map without judging the boundary through collisions or other sensors; in addition, some narrower entrances are known in advance during delineation and are not easy to miss in subsequent planned cleaning.

The invention discloses a distributed optical fiber and flow pressure value-based pipeline leakage joint detection method, which belongs to the field of pipeline leakage monitoring. A Sagnac interferometer principle-based distributed optical fiber pipeline leakage detection method is adopted in a ship pipeline and an optical switch is used at a light source outlet in place of an optical fiber coupler; complete light returning equipment is adopted at the tail end of the pipeline to replace an optical fiber ring structure; a flow sensor and a pressure sensor are arranged at the inlet of each section of pipeline respectively, and a pressure sensor is arranged at the outlet of each section of pipeline; an inlet flow threshold value and an inlet-outlet pressure difference threshold value are set for each section of pipeline; a phase difference characteristic signal and a flow and pressure variation signal generated by an optical fiber sensor are acquired to a computer and analyzed; when the two signals indicate that leakage occurs, the section of pipeline leaks; and when one signal indicates that the leakage occurs, the working condition of a system is continuously investigated on sitemanually so as to judge whether the leakage occurs. The method is suitable to be applied to the ship pipeline.

The invention relates to a trigger detection method for image acquisition applied for a high speed digital camera device. The method comprises the following steps that: (1), a high speed camera device carries out real-time and high speed shooting on images and stores the images to a dynamic memory; (2), an image acquisition module sends all stored real-time images to a detection area for detection; if a trigger requirement is met, an internal trigger signal is generated and then go to a nest step; if not, go back to the step (2) and detection is carried out continuously; (3), after the internal trigger signal is received, a time t 0 is generated by the trigger signal and is utilized as a trigger time; it is determined that images are needed high speed images, wherein the images are collected between a delta t 1 time before the trigger time and a delta t 2 time after the trigger; and then the images are marked and then are stored into the dynamic memory; and the high speed shooting at this time is completed; (4), the high speed camera device continuously carries out image acquisition and processes of the step (2) and the step (3) are repeated; when the storage space is not enough, newly collected images automatically cover the images that have been collected and stored before; but, the high speed images that have unique marks are reserved and are not covered.

The invention discloses a tunnel inspection image acquisition device and s tunnel inspection system. The tunnel inspection image acquisition device comprises a plurality of CD cameras, a plurality ofauxiliary light sources, and a fixed bracket, wherein the CD cameras and the auxiliary light sources are fixed on the fixed bracket, and fixing positions of the plurality of CCD cameras are arranged on the same circular ring; in a working state, the center of the circular ring is arranged at the circle center of a tunnel so as to ensure that the distances from the plurality of CCD cameras to a tunnel inner lining are identical, and the overall photographic angle of the plurality of CCD cameras cover the range of the tunnel inner lining. According to the tunnel inspection system, the tunnel inspection image acquisition device is arranged on a flaw detection mobile platform and cooperates with a computer, a display, a synchronous encoder and a data collector to perform synchronous photographing, disease identification, real-time stitching and display of the tunnel. The tunnel inspection image acquisition device and the tunnel inspection system have the technical features of wide detection range, fast detection speed and high timeliness.

The invention provides a water plugging agent for carbonatite and a preparation method and a using method thereof. The water plugging agent consists of the following raw materials in part by weight: 5 to 14 parts of cement, 4 to 8 parts of quartz, 0.1 to 0.5 part of cement flocculating agent, 4 to 11 parts of density lightening admixture, 1 to 2 parts of grid structure agent and 72 to 74 parts of water. The water plugging agent has high bonding strength and low density, and is suitable for high-temperature and high-mineralization carbonatite oil wells so as to solve the problem that the water plugging agent in the prior art is not suitable for the high-temperature and high-mineralization environment, has poor bonding strength and high density, and cannot effectively control water or improve the oil recovery ratio.

The invention relates to the field of a safety precaution monitoring technology, in particular to a linkage monitoring system and an implementation method thereof and is used for solving the problems that linkage monitoring, video recording and history examining of a plurality of monitoring devices can not be implemented when the alarming happens. The invention builds linkage relationships between a plurality of relevant monitoring devices and sets alarming linkage strategies, added detection conditions, and the like for relevant monitoring devices. When the alarming is detected, relevant monitoring devices are triggered for the operations such as the linkage monitoring and the storage of monitored audio / video according to the linkage relationships, alarming linkage strategies and added detection conditions. The invention implements the simultaneous cooperative monitoring and linkage of a plurality of monitoring devices and can more fully save monitoring data related to the alarming without easily missing important evidences. A synchronous historical playback technology of multiple pictures is adopted, which enables pictures related to the alarming to be extremely clear in the retrieval and second look of the historical video tapes and also overall alarming conditions can be analyzed and known more easily.

An embodiment of the invention provides an adhesive dispensing detection method, device and electronic device and relates to the field of battery modules. The method includes acquiring a to-be-detected image which is an image of a battery module panel subjected to adhesive dispensing employing adhesive added with a fluorescent agent, wherein the battery module panel in the to-be-detected image ispreset with mark information; judging whether the adhesive dispensing effect of the battery module panel is qualified or not according to the area occupancy of the fluorescent agent in the battery module panel; and storing the adhesive dispensing judgment result and the mark information. By adopting the method, device and electronic device provided by the invention, battery panel adhesive dispensing effect detecting efficiency can be improved and manual cost can be reduced.

The invention provides an SAR motion target detection method based on Doppler spectral analyses. The SAR motion target detection method based on Doppler spectral analyses is used for detecting ground motion targets in an SAR picture, and mainly solves the problem that an existing detection method is not high in detection probability so as to improve detection probability. According to the method, rectangular neighborhoods of scattering points in the SAR picture are chosen, clutter lock is conducted on Doppler frequency spectrums in each neighborhood to acquire Doppler center frequency, and then standard deviations of frequency spectrums after clutter lock are calculated to represent Doppler bandwidths. Traversal is conducted on all scattering points in the SAR picture, the scattering points are processed in the same way, statistics is then carried out, a measure is constructed through obtained statistics to ensure that the detection boundary of the measure is an oval, and a detection threshold is determined according to constant false alarm rate (CFAR). Because the detection boundary is an oval, on the premise that the constant false alarm rates are the same, oval detection boundary detection probability is larger than rectangle detection boundary detection probability, and target missing detection does not occur easily.

The invention discloses a photovoltaic terminal box, comprising a base, a box cover and a seal ring, wherein the base and the box cover carry out vertical extrusion and horizontal extrusion on the seal ring; terminal block components are arranged in the base and each terminal block component comprises a terminal main body, a wire clamp, a bus bar clamp and two diode clamps; a strut member is connected with a radiating component; an end face of each terminal main body is inserted into slots at two sides of the strut member and each terminal main body is riveted with the strut member; and the radiating components of the adjacent terminal block components are connected by heat conducting insulators and the radiating components and diodes are connected mutually by heat conducting insulators. The photovoltaic terminal box has high sealing property and a short terminal main body, and can be used for cooling and quick heat conduction.

The invention discloses an intelligent inspection robot and an inspection method. The intelligent inspection robot consists of a robot body, a mobile platform, a positioning navigation device, an automatic leveling device, a measurement instrument, an image collection device, a communication device, and a built-in server. According to the intelligent inspection robot, the robot performs inspectionaccording to a preset route through the positioning navigation device, staying at different preset detection positions for operation is performed, the self-leveling device provides a strictly horizontal basic platform for the measurement instrument, the robot guides the measurement instrument to measure a prism in the pipe tunnel, stores and calculates detection data, gives a settlement deformation measurement result, and can give a detection result of water leakage and other diseases, and the whole device can replace manual measurement, solves a problem of automatic detection of the pipe tunnel, and is convenient and fast to use and high in practicability.

The invention belongs to the technical field of textile engineering and provides a refining technology of silkworm oil rotten cocoons. The refining technology of the silkworm oil rotten cocoons is achieved through the following steps: primary refining cocoon boiling, biological decay, washing and dewatering, immersion cleaning, washing and dewatering, scouring and bleaching, washing and dewatering, secondary refining, washing and dewatering, softening, drying and cotton opening. By means of the refining technology, the biological decay enables silk fibers of the oil rotten cocoons to be fully dissociated and then mutually adhered together, accordingly short fibers are not prone to be omitted during later cocoon opening process, needle rollers are convenient to be hung, and use ratio of the silk fibers is improved. By adopting reasonable proportioning of various auxiliaries during different procedures, such as boiling refining, decaying, scouring and bleaching, damage to the silk fibers from chemical auxiliaries is reduced to the largest extent, oil removing effect is improved, sericin protective layers outside fibroins are reasonably protected, strength of the silk fibers is protected, comprehensive use ratio of oil rotten cocoons is improved, and the refining purposes including oil removing, rubber retaining, and fiber tensile force protection are achieved.

The invention provides a Taqman real-time fluorescence PCR (polymerase chain reaction) capable of reducing polymerization with primers. The Taqman real-time fluorescence PCR is improved Taqman and real-time fluorescence PCR which is in a hybrid stem structure combining a panhandle shape at the tail end of a molecular beacon and improves self combination. The Taqman real-time fluorescence PCR is characterized in that: fluorescein is labeled conventionally at 5'-terminal of a template probe sequence, complementary sequences of 5'-terminals of 5 to 6 probes are added to 3'-terminal of the template probe sequence, and a quencher is labeled at the tail terminal so as to form a stem structure similar to the tail terminal of the molecular beacon by hybridizing with the sequence at 5'-terminal of the probe. Two terminals of the probe are self-hybridized and combined, so that the quenching effect is increased, and the background is reduced; the probe is difficultly polymerized with a PCR primer due to self combination, so that primer-probe nonspecific polymerization amplification is eliminated, the detection sensitivity of the Taqman real-time fluorescence PCR is improved, and especially the relevance ratio of a weak-positive specimen is increased.

The invention discloses a method and a device for localizing software, and belongs to the technical field of a computer. The method comprises the following steps of disassembling the software into a common code part and a localized code part; carrying out the localized modification for the localized code part; and logically connecting the common code part with the localized code part after the localized modification to obtain the localized software. The device comprises a disassembling module, a localizing modification module and a logic connection module. When the localization of the software is realized, only fewer codes are changed, so that the workload is reduced, the time can be saved, and the place needing to be altered can be prevented from being missed; and since the common code part is identical, one localization software can be added only by copying one localized code part, no macro is added, the logic chaos of the software can be avoided, and the software is convenient to maintain.

The invention discloses a semantic-based candidate answer screening method and system for machine reading understanding. The method comprises the following steps of screening out the candidate answerfragments corresponding to a document from the document according to answers; screening out the optimal candidate answer segment from the candidate answer segments according to the semantic similarityand the answers; and obtaining a candidate answer document from the document according to the semantic similarity and the answers, and calculating to obtain the position information of the optimal candidate answer segment in the candidate answer documents, wherein the candidate answer document and the position information are the input values at a machine reading understanding model training stage. According to the present invention, the answers are screened according to the semantic similarity, screening is more comprehensive, the correct answers are not easy to miss, and the higher accuracyis achieved. The semantic-based candidate answer screening method and system for machine reading understanding can be widely applied to the field of natural language processing.

The invention relates to a method for evaluating the intrinsic quality stability of a cigarette blasting bead. The method comprises the following steps: grinding a plurality of batches of cigarette blasting bead samples separately, carrying out filtering to obtain blasting bead contents, adding an internal standard solution into the blasting bead contents, taking a supernatant after extraction, and filtering the supernatant to obtain filtrate, wherein the ratio of the blasting bead contents to internal standard substances in the internal standard solution is 1mL:(0.5-2.0)mg; carrying out gas chromatography-mass spectrometry analysis on the filtrate, taking an internal standard peak as a reference peak, determining a common peak, calculating a relative retention time alpha and a relative peak area S value of a chromatographic peak in the cigarette blasting bead sample, and establishing a chromatographic fingerprint of each blasting bead content sample; obtaining a euclidean distance, aeuclidean distance ratio and similarity by using the relative peak area S value of each component in each batch of cigarette blasting bead samples, and evaluating the intrinsic quality stability of the cigarette blasting bead. The method provided by the invention can comprehensively analyze the intrinsic quality of the cigarette blasting bead and reflect the fluctuation of the intrinsic quality.

The invention discloses a tile flatness detecting method and device. The device is characterized by comprising an upper linear guide rail and a lower linear guide rail which are horizontally arranged on the two sides of a tile conveying device, a vertical straight line guide rail perpendicular to the tile conveying direction, an inclined straight line guide rail corresponding to the tile diagonal direction, and laser displacement sensors which are arranged on the straight line guide rails in a sliding mode respectively, the detection direction of the laser displacement sensors faces downwards, and the laser displacement sensors on the straight line guide rails are driven by an electric power mechanism to move. Compared with the prior art, the tile flatness detecting method and device have the advantages that calibration is not needed and tile deformation detection omission is not prone to occurrence.

The invention relates to a strand substitute polymerase chain reaction, which is characterized in that a target gene chain to be detected is substituted by a probe containing reporter gene chain having a different sequence for indirect amplification, the probe sequence is complementary sequences selecting two small segments of conserved or specific sequences adjacent to target genes as double probes, different sequences of the reporter gene chain refers to a gene sequence which has minimum homology with the target gene chain or is in farther germ line, and the head and the tail of the reportergene chain are added with the given double-probe sequences to serve as linear band probe reporter genes. The target genes to be detected and the given head and tail probe sequences of the reporter genes are complementally hybridized so that the head and the tail of linear reporter genes are closed and linked, single-strand gaps of a hybrid are connected by ligase, and the target link is substituted by a reporter gene ring, namely a reverse PCR amplified reporter gene ring. By monitoring different sizes (at two ends) of reverse PCR amplification or the reporter genes with different codes, multi-target molecules can be indirectly indicated. More than one set of reporter genes can be alternately used.

The invention discloses a ground penetrating radar thin layer target identification and positioning method. The method comprises the following steps of (1) extracting each column successively from a target area of interest as an A-scan target; and carrying out synchrosqueezing short Fourier transform on each A-scan radar target data; (2) acquiring the time frequency energy information map of a signal; (3) according to energy distribution, finding a thin layer area; and (4) according to the time frequency energy information map, measuring the thickness of the thin layer to realize the positioning and the identification of a thin layer target. By using the method, data training does not need to be performed; thin layer target information can be completely reserved so as to increase the precision of target positioning, and for thin layer data with the layer thickness of lambda / 8, the thickness of the thin layer can be identified; thin layer identification precision can be increased, and astrong clutter and a target echo can be effectively distinguished; and an algorithm is simple, the operation time of the algorithm is short, and the accuracy of rapid thin layer target positioning isensured.

The invention discloses a casting continuous cleaning flow line and a method thereof. The casting continuous cleaning flow line comprises a bottom plate, an upper flow line and a lower flow line, wherein the upper flow line comprises a bottom plate feeding station, a workpiece loading station, a primary cutting station, a primary gouging station, an overturning station, a secondary cutting position, a secondary gouging station, a workpiece unloading station, a bottom plate automatic cleaning and returning station which are arranged in sequence; the bottom plate moves along the stations; the lower flow line is connected with the upper flow line end to end to form a closed operation loop; and through the lower flow line, the bottom plate can be transmitted from the bottom plate automatic cleaning and returning station to the bottom plate feeding station. The casting continuous cleaning flow line and the method thereof have the advantages that the various defects of the existing manual cleaning flow line are overcome, the mechanization and the pipelining of cleaning works of large quantities of steel castings are realized, cutting scraps are effectively and quickly collected, the whole equipment is safe and reliable, is long in service life and is convenient and simple to operate and maintain, and the work site area is saved.

The invention relates to a steel pipe automatic drilling robot. The steel pipe automatic drilling robot comprises an execution mechanism and a steel pipe body; the execution mechanism butts against the outer wall of the steel pipe body; the steel pipe body is of a hollow cylinder structure; an execution cylinder drives an inclined hole drill bit, mounted on an output shaft of a drilling driving motor, to drill and feed through extension control; the drilling driving motor drives the inclined hole drill bit to rotate for drilling; an adjusting cylinder drives the execution cylinder to perform an arc motion through extension control to achieve the effect of adjusting the drilling angle; and an auxiliary spring cooperates with an auxiliary extension rod to fix a positioning plate and to achieve a damping effect during drilling. The steel pipe automatic drilling robot can solve the problems of inconvenience for manual operation in multi-angle drilling, time and manpower waste, instable drilling, easy deflection of inclined holes, influence on the drilling quality and use, incapability of drilling holes on the same steel pipe by multiple angles through traditional drilling machinery, and the like.

The application discloses a page positioning method and a page positioning device for error input information. The method comprises the steps of submitting all input information in a page to a background for checking error; determining the error input information in the page according to an error checking result returned by the background; according to all the determined error input information, generating and displaying error prompt superposed layers including prompt information for all the error input information; positioning the page according to anchor points corresponding to page input areas where all the error input information is positioned. After the method and the device are adopted, a user can know all the error input information on the page by means of the error prompt superposed layers, and the page input areas, where the error input information is positioned, on the page can be positioned according to the anchor points, so that the user can conveniently modify the error input information; therefore, the method and the device can simplify the user operation and enable the user not to easily miss the error input information.

The invention discloses a method for screening hybridoma cells secreting specific monoclonal antibodies, and an application thereof. The method comprises the following steps: coupling Oleyl-PEG4000-NHS with Ag to obtain Oleyl-PEG4000-NHS-Ag; adding Oleyl-PEG4000-NHS-Ag in cloning of hybridoma cells to be screened, culturing, removing the above obtained supernatant, and cleaning; adding a fluorescent substance-labeled anti-mouse second antibody, incubating, removing the obtained supernatant, and cleaning; and adding an incomplete medium, observing cell masses under visible light and fluorescence by using an inverted fluorescence microscope, carrying out negative and positive labeling, adding a methyl cellulose semisolid medium to plate holes containing positive cell masses, transferring positively labeled cells to a new cell culture plate, and culturing to obtain the hybridoma cells secreting the specific monoclonal antibodies. The method has the advantages of rapidness, simplicity and high accuracy, and can be used for rapid screening to obtain the hybridoma cells secreting the specific monoclonal antibodies.