8results about How to "Increase positive rate" patented technology

The application provides an intelligent agent-based whole-process antibody drug design optimization system and method, which comprises the following steps: receiving an antibody design requirement input by a user through an intelligent agent, performing intention recognition, task classification and parameter extraction on the input by the intelligent agent, and automatically constructing an antibody design task plan; structural modeling and combination hotspot analysis; generating a brand new antibody skeleton or optimizing an existing antibody skeleton; CDR sequence filling and candidate sequence generation; structure verification and affinity evaluation; humanization design and developability evaluation; candidate antibody sorting and result output; and generating a comprehensive design report. The application is used for guiding a user to complete a complete process from a target point to a high-affinity humanized antibody candidate sequence.

This invention discloses the application of ABCC1 inhibitors in the preparation of drugs for treating nasopharyngeal carcinoma. Immunohistochemical experiments revealed that ABCC1 protein expression in nasopharyngeal carcinoma tumor tissues is higher than in adjacent normal tissues. The positive rate of ABCC1 staining in advanced-stage patients is significantly higher than in early-stage patients, and the proportion of patients with high ABCC1 expression is also significantly higher in patients with poor prognosis than in patients with good prognosis. Therefore, this invention further designs ABCC1 inhibitors targeting ABCC1, finding that knocking down or silencing ABCC1 significantly inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells; simultaneously, inhibition of ABCC1 function effectively enhances CD8... + The immune effector function of T cells. Therefore, ABCC1 can be used in the diagnosis and treatment of nasopharyngeal carcinoma.

The application belongs to the technical field of biology and specifically relates to an anti-CD25 monoclonal antibody, a preparation method thereof and application thereof. The anti-CD25 monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region VH comprises three complementarity determining regions, and the amino acid sequences are shown as SEQ ID NO: 1-3. The light chain variable region VL also comprises three complementarity determining regions, and the amino acid sequences are shown as SEQ ID NO: 4-6. The application further provides a preparation method of the anti-CD25 monoclonal antibody. The monoclonal antibody development method has the advantages of short time consumption, high throughput and high positive rate.

The application provides a Lemna australis endogenous strong promoter, a recombinant vector containing the Lemna australis endogenous strong promoter, and application of the Lemna australis endogenous strong promoter and the recombinant vector in starting expression of a target gene in monocotyledonous plants and dicotyledonous plants. The Lemna australis endogenous strong promoter provided by the application has high activity in monocotyledonous plants and dicotyledonous plants, can maintain high activity under conditions of different concentrations of screening agents, and basically does not affect expression of adjacent genes under conditions of different concentrations of screening agents. The application can solve problems of the 35S promoter widely used in the prior art, such as difficulty in further improving activity, low activity in monocotyledonous plants, and influence on expression of adjacent genes.

This invention discloses a nucleic acid molecule encoding a modified retroviral Gag protein and its applications. The Gag protein contains an engineered acylated tag sequence at its N-terminus, comprising: a glycine residue at the second position after the initiating methionine for myristylation modification; and / or a cysteine ​​residue in the N-terminal region for palmitoylation modification. The tag sequence is selected from SEQ ID NO:1, 3-9, or derivative amino acid sequences having at least 80% identity and retaining function. Preferably, the modification involves replacing the first 9 amino acids at the N-terminus of the wild-type HIV-1 Gag protein with the aforementioned tag to enhance membrane anchoring and improve viral packaging efficiency. This invention also provides packaging plasmids containing this molecule, host cells, packaging systems, and preparation methods. This invention can significantly improve viral yield and titer, and is particularly suitable for overcoming the efficiency bottleneck in packaging large-load lentiviruses of 7-10 kb.

The utility model discloses a multi-range gynecological cell sampler and a sample box, and belongs to the field of cervix sampling. The bottom plate is arranged at the head end of the holding rod; the insertion rod is arranged on the bottom plate; two inserting shafts are arranged on the outer wall of the top end of the inserting rod; the side sampling piece and the upper sampling piece are detachably arranged on the insertion rod respectively; the upper sampling piece comprises an upper brush head which is crown-shaped, two wings of the upper brush head protrude towards two sides, and bristles in the middle of the upper brush head are longer than the two wings. According to the multi-range gynecological cell sampler and the sample box, through the arrangement of the side sampling piece and the upper brush head, the cervical orifice, the fornix beside the cervical orifice and the vagina can be sampled at the same time, and compared with the prior art, the material consumption is reduced, the sampling process is greatly simplified, and the sampling efficiency is improved. Meanwhile, as the two wings of the upper brush head protrude towards the two sides and the middle bristles are longer than the two wings, the two protruding wings can reduce the contact probability of the middle bristles (cervical orifice samples) and the vagina wall, and therefore the positive rate of certain TCT can be increased.

The application relates to the technical field of medicines, in particular to application of a VER155008 compound in preparation of a coronavirus-dependent RNA polymerase antagonist. Through construction of a reliable screening model of SARS-CoV-2 RdRp inhibitors, the application tests the inhibiting effect of a plurality of nucleoside analogs on SARS-CoV-2 RdRp, finds that the VER155008 compound has obvious inhibiting effect on SARS-CoV-2 RdRp, the VER155008 compound has SARS-CoV-2 exonuclease nsp14 resistance, and can effectively inhibit replication of coronaviruses HCoV-OC43 and HCoV-NL63. Therefore, the application provides key data for research and development of a novel anti-new coronavirus lead compound, and provides a theoretical basis for research and development of an effective coronavirus treatment drug.

The invention belongs to the technical field of medical instruments, and particularly discloses a miniature fluorescence microscopic imaging and automatic counting system for onychomycosis detection. The system comprises an integrated handheld sampling imaging terminal, a cloud intelligent analysis server and a user interaction terminal. The terminal integrates sampling, fluorescence labeling and microscopic imaging functions, automatically completes sample processing and image acquisition, and uploads the sample processing and image acquisition to the cloud. And the server performs automatic identification, segmentation and counting of fungal targets on the image through a deep learning algorithm to generate a detection report. According to the invention, mechanical sampling, microfluidic fluorescence labeling and miniature fluorescence microscopic imaging are highly integrated in the handheld terminal, a closed and automatic sample processing and imaging assembly line is constructed, and the problem of leak detection caused by insufficient sampling depth and inconsistent dyeing operation in a traditional method is solved.