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Probe set and kit for detecting whole exons of extended genetic diseases and application of probe set
InactiveCN110499364AComprehensive diagnostic extended whole exome testingIncrease positive rateMicrobiological testing/measurementLibrary creationFresh TissueExon
The invention discloses a probe set for detecting whole exons of extended genetic diseases. The probe set for detecting the whole exons of the extended genetic diseases comprises a standard whole exonprobe set, a whole genome copy number variation probe, and a mitochondrial loop full-length probe, and the genetic diseases comprise 6161 genetic diseases; the standard whole exon probe set can detect the genetic diseases caused by whole exon mutation, the genetic diseases comprise nervous system diseases, metabolic system diseases, endocrine system diseases, digestive system diseases, skeletal system diseases, urinary system diseases, immune system diseases, cardiovascular system diseases, blood system diseases, integument system diseases, ophthalmic system diseases, ear system diseases, respiratory system diseases, and genital system diseases; and the density of the mitochondrial probe is 6X; test samples comprise blood, fresh tissue, FFPE samples, and saliva. The invention discloses using method and kit and application of the probe set for detecting the whole exons of the extended genetic diseases.
Owner:北京凯昂医学诊断技术有限公司
Molecular cloning method based on CRISPR/Cas9 and homologous recombination of saccharomyces cerevisiae cell endogenous genes
ActiveCN105624146AStrong specificityEasy to fragmentFungiVector-based foreign material introductionTime transformationDNA fragmentation
The invention relates to a novel molecular cloning method, and in particular to a method for obtaining recombinant vectors by modifying initial vectors through combined utilization of a CRISPR / Cas9 system and a saccharomyces cerevisiae cell endogenous gene homologous recombination system; only through one-time transformation and selection operations, the method can simultaneously complete insertion of ore or more target DNA fragments into the initial vectors, deletion of one or more DNA fragments from the initial vectors and / or substitution of one or more DNA fragments on the vectors for one or more target DNA fragments. The invention further relates to a reagent kit for conveniently and effectively applying the method provided by the invention.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Method for constructing Glrx1 gene knock-out animal model based on CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 technology
ActiveCN107287245AIncrease positive rateLow miss rateHydrolasesStable introduction of DNAF1 generationBioinformatics
The invention discloses a method for constructing a Glrx1 gene knock-out animal model based on a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas9 technology. The method comprises the following steps: 1, selecting and designing gRNA of Glrx1 genes of targeted mice; 2, constructing an sgRNA vector; 3, performing in vitro transcription on sgRNA; 4, injecting one cell stage fertilized eggs of mice; 5, birth and identification of F0-generation mice; and 6, reproducing positive F0-generation mice, and realizing birth and identification of F1-generation mice. According to the method disclosed by the invention, the Glrx1 gene knock-out animal model can be successfully obtained.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for efficiently separating single antigen-specific B lymphocyte from spleen cells
ActiveCN110016462AIncrease positive rateKeep activeCell dissociation methodsBlood/immune system cellsSurface markerSpleen cell
The invention discloses a method for efficiently separating single antigen-specific B lymphocyte from spleen cells. High-throughput rapid screening of single antigen-specific B lymphocyte is realizedby negative screening adopting multiple lymphocyte surface marker antibodies, establishment of a rapid fluorescence marked antigen substance method and high-specificity screening by use of a fluorescence marked antigen substance, and the positive rate of the finally obtained antibody-specific B lymphocyte is increased from less than 5% in a traditional hybridoma method to 30%. Therefore, the scheme can shorten monoclonal antibody development time to a great extent and greatly increase the number and diversity of obtained monoclonal antibodies.
Owner:优睿赛思(武汉)生物科技有限公司
High accuracy detection method of human papilloma virus genotype
InactiveCN102367487AHigh detection sensitivityImprove featuresMicrobiological testing/measurementMaterial analysis by electric/magnetic meansCapsidMass spectrometric
The invention provides a high accuracy detection method of human papilloma virus genotype. Double probe, which comprises a carcinogenicity early transcription region E6 or E7 gene region and a capsid protein late transcription region L1 or L2 gene region, is adopted to detect every genotype. According to the method, pollution exclusion of PCR amplification product is taken as a premise, identical genotype recognition by double gene probe is taken as a basis, and high sensitivity PCR-mass spectrum combination is taken as a platform. The invention provides a method for high accuracy detection and / or identification of human papilloma virus (HPV) genotype.
Owner:INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI +1
Microbial sample processing system
InactiveCN103194385AEnsure biosecurityImprove airtightnessBioreactor/fermenter combinationsBiological substance pretreatmentsTemperature controlMicroorganism
The present invention provides a microbial sample processing device, comprising: a machine body provided with a machine chamber and a sealed movable door; and a sealed housing, a stretchable airbag, a sample tray, a mechanical arm, a bar code scanner, a liquefier, culture dishes, a gas-temperature sensor, and a temperature control device which are arranged in the machine chamber, wherein the sample tray is provided with a plurality of sample seats, the sample seats are used for placing the culture dishes, the liquefier is communicated with the culture dishes, each culture dish is provided with culture plates and a streak-inoculation pen used for streak inoculating the sample in the culture dishes from the liquefier on the culture plate. The microbial sample processing device is reasonable in structure, fast and safe, reliable and efficient, can complete the whole process of mixing and liquefying, streak inoculation, dilution and separation, and incubation of microbial community automatically in a one-stop manner, and can replace manual labor and ensure biological safety.
Owner:湖南千山医疗器械有限公司
Kit for detecting klebsiella pneumoniae
ActiveCN104946762ASimplify testing proceduresShort detection cycleMicrobiological testing/measurementDNA/RNA fragmentationK pneumoniaeNucleotide
The invention discloses a kit for detecting klebsiella pneumoniae, and belongs to the technical field of PCR detection. The kit comprises specific primers and a probe for detecting klebsiella pneumoniae; the nucleotide sequences of the specific primers and the probe are as shown in SEQ ID NO.1-3; a detected target gene is a sequence of a pho gene, and has a nucleotide sequence as shown in SEQ ID No.4. The kit has the advantages of being accurate in detection, high in sensitivity, strong in specificity, and simple and quick to operate, has a favorable sample detection capacity, can replace a traditional bacterial isolated cultivation and diagnosis method, and the novel kit for quick detection is provided for klebsiella pneumoniae.
Owner:BIOSINO BIO TECH & SCI
Fungus detection fluorescent dyeing liquid and use thereof
InactiveCN106198470AClear structureEasy to findPreparing sample for investigationFluorescence/phosphorescenceParaffin waxFluorescence
The invention discloses a fungus detection fluorescent dyeing liquid and a use thereof. The fungus detection fluorescent dyeing liquid comprises an independent dyeing liquid A and an independent re-dyeing liquid B. The dyeing liquid A comprises, by weight, 0.02-0.043% of a fluorescent brightener, 1-10% of potassium hydroxide, 10-20% of dimethyl sulfoxide, 0-5% of glycerin and 65-87% of water. The re-dyeing liquid B comprises, by weight, 0.1-0.5% of Evans blue and 99.50-99.90% of water. The invention discloses a use of the fungus detection fluorescent dyeing liquid. The invention provides a novel fast fungus infection detection product. The product can be used for detecting various fungi which may exist in a fresh or frozen clinical sample and paraffin and ethylene glycol methacrylate-coated tissue.
Owner:JIANGSU LIFETIME BIOLOGICAL TECH
Gene polymorphism sites related to thyroid cancer and application thereof
PendingCN107164496AStrong specificityExcellent diagnostic valueMicrobiological testing/measurementMaterial analysisGenes mutationSomatic cell
The invention provides somatic mutation sites of pathogenic genes of the thyroid cancer and application thereof, specifically to a group of mutation sites of the pathogenic genes of the thyroid cancer. The mutation sites are composed of a GNAS gene mutation site, a NRAS gene mutation site, a TSHR gene mutation site, etc. The gene mutation sites provided by the invention can be used as markers for identification of benign and malignant thyroid nodules.
Owner:上海安甲生物科技有限公司
Method and kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation
InactiveCN101831490AStrong complementarityWide coverageMicrobiological testing/measurementBiologyCDH1
The invention relates to a method and a kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation. Particularly, the invention provides a kit, which contains (a) a primer pair aiming at methylation specificities and non-methylation specificities of a POX1 gene and a CDH1 gene, or contains (b) a methylation sensitivity restriction endonuclease, and a primer pair aiming at the specificities of the POX1 gene and the CDH1 gene. The kit can effectively separate the cervical carcinoma from cervicitis and HPV infection.
Owner:上海市第八人民医院
Wearable digital electroencephalogram monitoring helmet
PendingCN107714035AEEG monitoring simplified and convenientEnsure your own safetySensorsTelemetric patient monitoringEarly warning systemHigh level analysis
The invention belongs to the technical field of medical apparatuses, and in particular relates to a wearable digital electroencephalogram monitoring helmet, which comprises acquisition electrodes, emitting systems, a helmet abnormal electroencephalogram recognition and alarm system, a helmet shell, a monitoring terminal system and a remote advanced electroencephalogram epileptiform discharge automatic analysis recognition and early warning system, wherein the acquisition electrodes are fixedly connected to the inner side of the helmet shell; the emitting systems are fixedly connected to two sides of the helmet shell; and the helmet recognition and alarm system is fixedly arranged in the helmet shell. The problem that effective protective and treatment measures cannot be adopted timely since it is low in rate of discovering the positive rate of epileptiform attack in a conventional electroencephalogram and monitoring and alarming effects for epileptiform of a patient are in lack can besolved; the helmet provided by the invention, on the basis of double early warning measures, namely simple alarming and remote advanced analysis recognition alarming, can timely and precisely discoveroncoming attack and in-attack of patient's epilepsy and can detect the positive rate of epileptiform discharge, so that preventing and alarming effects can be achieved, and subsequently effective protective and treatment measures can be adopted timely so as to protect patient's security.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Multi-element generic composition and use thereof
ActiveCN104745681AOvercoming the problem of poor specificityIncrease positive rateMicrobiological testing/measurementDNA/RNA fragmentationMulti elementMethylation
The invention provides use of a composition for preparing a kit for detecting cell proliferative abnormalities of an individual. The composition comprises a nucleic acid for detecting the methylation level of Septin9 and RNF180 genes as well as at least one target region in the fragments of the genes. Therefore, existence of cell proliferative abnormalities is indicated by virtue of integrating the methylation detection results of Septin9 and RNF180.
Owner:BIOCHAIN BEIJING SCI & TECH +1
Method for enriching and purifying blood platelets
ActiveCN104307208AImprove enrichment purityReduce pollutionMammal material medical ingredientsNon-miscible liquid separationPurification methodsWhite blood cell
Owner:杭州三江上御生物科技有限公司
Kit for detection of D. destructor in sweet potato based on loop-mediated isothermal amplification and its application
ActiveCN102260746AAvoid time lossShorten the timeMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceDitylenchus destructor
The invention discloses a kit for detecting Ditylenchus destructor based on loop-mediated isothermal amplification and application thereof. The invention provides a special primer for assistant identification of Ditylenchus destructor and consists of DNAs shown in sequences 1-14 in a sequence table. The kit provided by the invention comprises the special primer. According to the invention, the kit for assistant identification of Ditylenchus destructor is provided through designing the specific primer and optimizing reaction conditions; and through applying the kit, an interspecies level and an infraspecies level of detection can be achieved, and the problems that the routine molecule detection method has the defects of high cost, low efficiency and slow speed and can not meet the requirements of current plant quarantine and plant protection are solved.
Owner:CHINA AGRI UNIV
Tuberculosis antibody multi-antigen ELISA detecting kit and making method
The invention relates to a tuberculosis antibody multiple antigen ELISA detection kit and a preparation method thereof, which pertains to the field of tuberculosis medical immunology diagnostic techniques and mainly uses detection antigen, enzyme-linked antihuman IgG antibodies, substrates, positive control serum of tuberculosis patients, control serum of normal person, calf serum and polystyrene microplates to form the kit, wherein, the detection antigen adopts the mycobacterium tuberculosis complex strains of lipid Arabian mannose (LAM), 38kD and 16kD to be combined with arbitrary one or more than one mycobacterium tuberculosis recombinant proteins in the recombinant proteins of MPT63, MTB48 and CFP10-ESAT6. The mycobacterium tuberculosis has high sensitivity, strong specificity and complementarity, can be used for detecting specific antitubercular antibodies in such body fluid samples as serum, hydrothorax and the like, and assisting the diagnosis and differential diagnosis of tuberculosis.
Owner:中国人民解放军总医院第二附属医院
Primer pair, kit and method for methylation detection of stomach cancer related genes Reprimo and RNF180
InactiveCN107904313AEasy to useReliable test resultsMicrobiological testing/measurementDNA/RNA fragmentationForward primerRibonucleoside
The invention relates to a primer pair for methylation detection of stomach cancer related genes Reprimo and RNF180. The primer pair comprises forward primers, reverse primers and detection probes ofReprimo, RNF180 and beta-Actin. The invention relates to the kit for methylation detection of the stomach cancer related genes Reprimo and RNF180. The kit comprises a PCR (polymerase chain reaction) solution of the primers and the probes, and the PCR solution comprises the forward primers, the reverse primers, the detection probes, a 10*PCR buffer, dNTP (deoxy-ribonucleoside triphosphate), Mg<2+>,nuclease-free water and an Ex Taq enzyme. The invention further relates to the method for methylation detection of the stomach cancer related genes Reprimo and RNF180. The kit and the detection method thereof have the advantages of accurate detection result, high sensitivity, high flux, high specificity and short detection time.
Owner:韩林志
Infectious clone vector of melon necrotic spot virus and construction method of infectious clone vector
ActiveCN105296526AIncrease positive rateAvoid restrictionsBacteriaMicrobiological testing/measurementNicotiana tabacumCloning vector
Disclosed is an infectious clone vector of a melon necrotic spot virus. The infectious clone vector pCB301-MNSV is obtained by recombination of a melon necrotic spot virus whole genome and a plant expression vector pCB301-2x35S-MCS-HDVRZ-NOS with a 35S promoter and ribozyme Rz. The infectious clone vector pCB301-MNSV is transferred into agrobacterium GV3101 competent cells through a freeze-thaw method, and then a melon seedling is inoculated to detect infectivity of the pCB301-MNSV through an agrobacterium injection method. The infectious clone vector is expressed in agrobacterium strongly, is capable of infecting cucurbit plants such as melons, watermelons and cucumbers but cannot infect tobacco, animals and humans, and accordingly relative safety is achieved and studies on viruses can be operated on the DNA (deoxyribonucleic acid) level easily because of infectious cloning success.
Owner:ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
Intelligent pre-examination method and device for traffic illegal pictures
InactiveCN109344805AImprove recognition accuracyIncrease the intelligent pre-examination linkRoad vehicles traffic controlCharacter and pattern recognitionVehicle behaviorSimulation
The invention provides an intelligent pre-examination method and a device for traffic illegal pictures, belonging to the technical field of traffic picture examination. The method comprises the following steps of: identifying various vehicle information in a picture according to a picture taken by a road monitoring device; Identifying vehicle behavior in the picture; Selecting a target picture according to the vehicle behavior in the picture, and recording vehicle information of the target vehicle in the target picture; The target picture is sent to the local law enforcement platform to enterthe manual review stage. The invention utilizes intelligent pre-examination technology to identify vehicle behavior, Fast screening of off-site law enforcement data is realized, and intelligent pre-examination of pictures is added before manual examination, which improves the identification accuracy of illegal pictures, reduces the manual examination workload, increases the positive rate of off-site law enforcement pictures, and improves the work efficiency of law enforcement personnel.
Owner:BEIJING SEEMMO TECH CO LTD
Zymomonas mobilis genome editing method based on CRISPR-Cas12a system and application of method
ActiveCN110358767ASimple and fast operationIncrease positive rateBacteriaStable introduction of DNAHeterologousCompetent cell
The invention belongs to the technical field of gene editing, and particularly relates to a Zymomonas mobilis genome editing method based on a CRISPR-Cas12a system and application of the method. The method for editing a Zymomonas mobilis genome is established on the basis of the CRISPR-Cas12a system with Zymomonas mobilis (ZM4) as the modulus strain, the oriented editing of the genome is realized,a gene editing tool is provided for developing the rational design of an allogenic metabolic pathway and cell factory for producing biomass fuel and biological materials in the strain, and the development in metabolic engineering and related research fields is promoted. The method is technically characterized by including the steps of establishing an inducible expression Cas12a recombinant strainand an editing plasmid containing an artificial CRISPA expression unit, designing guiding RNA, connecting the guiding RNA to the editing plasmid after annealing the primer sequence of the guiding RNA, and shifting a target plasmid into a competent cell for expression editing.
Owner:武汉睿嘉康生物科技有限公司
Plasmid vector and method for building plant population by using plasmid vector
ActiveCN108034671AIncrease positive rateReduce the probability of false positive plantsHydrolasesVector-based foreign material introductionOrigin of replicationPlant population
The invention relates to a plasmid vector and a method for building a plant population by using the plasmid vector. The plasmid vector contains, at a replication origin, a gene expression box for expressing Cas9 protein, a first promoter, nucleotide sequences containing suicide gene sequences, a gRNA scaffold element and a termination signal, and at least one first enzyme cutting site located on the nucleotide sequence 5' end containing the suicide gene sequence and at least one second enzyme cutting site located at the nucleotide sequence 3' end containing the suicide gene sequence; furthermore, no first enzyme cutting site and no second enzyme cutting site exist in other positions of the plasmid vector. The first promoter is located at the upstream of the termination signal; the nucleotide sequences containing suicide gene sequences and the gRNA scaffold element are all located between the first promoter and the termination signal.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Primers, probes, methods and kits for detecting campylobacter jejuni
InactiveCN102268478AIncrease positive rateJudging the degree of pollutionMicrobiological testing/measurementFluorescence/phosphorescenceFluorescenceMicrobiology
The invention provides real-time fluorescence quantitative PCR (Polymerase Chain Reaction) primers and probe for detecting campylobacter jejuni and a method for carrying out quantitative detection by using the primers and probe. The nucleotide sequences of the primers and probe are respectively shown in SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention also provides a kit for detecting campylobacter jejuni. The detection method and the kit thereof provided by the invention have the advantages of accurate detection, high sensitivity, strong specificity, simplicity, convenience, quicknessand good clinical specimen detection capability.
Owner:ICDC CHINA CDC
Transformation method utilizing red fluorescent protein as selection marker of rice transformation
ActiveCN102199619AImprove reliabilityIncrease positive rateVector-based foreign material introductionBiotechnologyFluorescence microscope
The invention provides a transformation method utilizing a red fluorescent protein as a selection marker. The method comprises the following steps of: synthesizing a fluorescent protein (FP) gene and modifying according to the bias of a rice codon, wherein the modified FP gene is used as a selective marker gene of rice transformation and plant regeneration; driving the FP gene by a callus / seed coat-specific promoter, closely connecting with a target gene, and transferring to rice embryonic calli under the mediation of Agrobacterium tumefaciens; screening for three times by a fluorescence microscope in 30 days after coculture; and further verifying an obtained transgenic seedling by utilizing a polymerase chain reaction (PCR) and Southern hybridization. The result shows that the positive rate of the transgenic seedling is up to 80 percent, which proves that FP serving as the selection marker of plant transformation is completely feasible. By the method, the potential hazard of the traditional selection markers such as antibiotic and herbicide resistance genes and the like to environment and food safety is effectively reduced. The invention provides a novel safe plant genetic transformation screening system.
Owner:BEIJING WEIMING KAITUO CROP DESIGN CENT COMPANYLIMITED +1
Novel aerosol and droplet coronavirus pathogen collection mask and collection method
PendingCN111513377AIncrease positive rateAchieve mass productionProtective garmentSpecial outerwear garmentsAntibiosisVirus
The invention discloses a novel aerosol and droplet coronavirus pathogen collection mask and a collection method. The mask comprises ear hooks, a mask body and a breather valve on the mask body, the breather valve comprises an antifouling layer, a filtering layer, a heat preservation layer, an antibacterial virus layer and a skin-friendly layer, and the breather valve further comprises a pathogencollecting layer which sequentially comprises the antifouling layer, the filtering layer, the antibacterial virus layer, the heat preservation layer, the pathogen collecting layer and the skin-friendly layer from outside to inside. The invention realizes a new sampling mode which is convenient and simple to use and can realize aerosol and droplet pathogens and even sputum, and is beneficial to saving medical resources and improving the positive rate of samples. A breather valve of an existing KN95 mask is modified into a detachable valve with filtering and enriching functions, and large-scaleproduction of the breather valve can be achieved. Production difficulty is low, and the minvention is simple and easy to implement.
Owner:广州市宝创生物技术有限公司
Method for efficiently isolating single antigen-specific b-lymphocytes from spleen cells
ActiveCN110016462BHigh purityGuaranteed monoclonalityCell dissociation methodsBlood/immune system cellsSurface markerSpleen cell
The invention discloses a method for efficiently separating single antigen-specific B lymphocyte from spleen cells. High-throughput rapid screening of single antigen-specific B lymphocyte is realizedby negative screening adopting multiple lymphocyte surface marker antibodies, establishment of a rapid fluorescence marked antigen substance method and high-specificity screening by use of a fluorescence marked antigen substance, and the positive rate of the finally obtained antibody-specific B lymphocyte is increased from less than 5% in a traditional hybridoma method to 30%. Therefore, the scheme can shorten monoclonal antibody development time to a great extent and greatly increase the number and diversity of obtained monoclonal antibodies.
Owner:优睿赛思(武汉)生物科技有限公司
Liquid injection and suction method for cell slide-making dyeing machine and device thereof
ActiveCN103543058AIncrease positive rateAvoid residual wastePreparing sample for investigationDyeingEngineering
The invention discloses a liquid injection and suction method for a cell slide-making dyeing machine. The liquid injection and suction method comprises the following steps of 1) injecting a proper amount of a body fluid sample, which is required for detection, in a sampling bottle onto a glass slide through a liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of a round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid injection pipe during liquid injection; 2) sucking away redundant waste liquid through a liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe; 3) injecting dyeing liquid required for dyeing through the liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of the round pipe wall, stewing the dyeing liquid, and titling the glass slide towards one side of the round pipe wall approaching the liquid injection pipe; 4) sucking away the redundant waste liquid through the liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall after cells are colored, tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe when the redundant waste liquid is sucked away, and repeating the step 3) and the step 4) for 2-8 times until the dyeing operation is finished.
Owner:天津百利鑫生物科技有限公司
A method for rapidly obtaining transgenic plants of capsicum
InactiveCN102286526AShorten inoculation regeneration culture timeImprove conversion efficiencyGenetic engineeringFermentationTransgenesisScreening cultures
The invention relates to a method for quickly obtaining a capsicum transgenic plant, which comprises the following steps: sampling; activating and infecting agrobacterium; preparing a regeneration culture medium and a screening culture medium; inoculating the immature embryo, and culturing; and forming adventitious bud and regenerating the plant, thereby efficiently obtaining the capsicum transgenic regenerated plant. In the invention, it usually takes 7 weeks from the immature embryo infected by agrobacterium to the acquisition of the regenerated plant, the formation rate of the regenerated plant in the culture process is up to 50% to the maximum, and the positive rate of the transgenic plant is up to 80%. In the invention, the immature embryo is used as a receptor material to infect theagrobacterium and carry out inoculation and culture so as to directly form the regenerated plant, thereby shortening the culture period and enhancing the culture effect and genetic transformation efficiency. If applied to genetic functional verification and breeding practice, the invention can greatly accelerate the research into capsicum functional genomics and the development of breeding practice.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Bovine, goat, porcine and canine brucella typing fluorescent PCR (polymerase chain reaction) detection reagent kit and preparation and application thereof
ActiveCN105624303AAccurate typingShort experiment cycleMicrobiological testing/measurementMicroorganism based processesTypingFluorescent pcr
The invention belongs to the field of biotechnical detection, and particularly relates to a bovine, goat, porcine and canine brucella typing fluorescent PCR (polymerase chain reaction) detection reagent kit and preparation and application thereof. The bovine, goat, porcine and canine brucella typing fluorescent PCR reagent kit contains bovine brucella detection primers and probes, goat brucella detection primers and probes, porcine brucella detection primers and probes and canine brucella detection primers and probes. The bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit, the preparation and the application have the advantages that the bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit is high in detection sensitivity, the lowest detection limit is 1*10<3> copy / ml, and the accuracy and the positive rate can reach 100%.
Owner:广东省疾病预防控制中心 +1
Primers and probe for detecting peste des petits ruminants virus and kit
InactiveCN102676697ASimplify testing proceduresIncrease positive rateMicrobiological testing/measurementFluorescence/phosphorescenceNucleotideFluorescence
The invention provides a genetic marker, real-time fluorescence quantitative PCR (polymerase chain reaction) primers and a probe used for detecting peste des petits ruminants through the N gene sequencing and comparison of the peste des petits ruminants, and the nucleotide sequences of the genetic marker, the primers and the probe are respectively disclosed in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention also provides a quantitive detection method and a detection kit for the peste des petits ruminants virus. The detection method and the detection kit disclosed by the invention have the advantages of accuracy in detection, high flexibility, strong specificity, easiness and rapidness, and the specimen detection capacity is good.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
K subgroup avian leukosis virus detection kit
InactiveCN108048603AQuick checkAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationDisease monitoringLeucosis
The invention discloses a K subgroup avian leukosis virus detection kit, which relates to the technical field of virus fluorescent quantitative PCR detection. The kit of the invention comprises one pair of specific primers, and nucleotide sequences of the specific primers are respectively as shown by SEQ ID No. 2 and 3. The kit of the invention is simple in application method, low in cost, easy inobserving a reaction result, high in sensitivity, high in specificity, capable of rapidly detecting K subgroup avian leukosis viruses, very suitable for disease monitoring, on-site emergency and clinical sample detection and suitable for large-scale popularization and application.
Owner:SOUTH CHINA AGRI UNIV
Method for detecting activity of spermidine/spermine N1-acetyltransferase
InactiveCN102344950AHigh selectivityThe method is safe and effectiveMicrobiological testing/measurementN acetylationMetabolite
The invention, relating to the technical field of medicine, relates to a method for detecting the activity of spermidine / spermine N1-acetyltransferase, that is, a quantitative technique of analyzing SSAT activity through detecting acetylated metabolites of the SSAT substrate. According to the invention, the N-acetylation is regulated mainly through or only through SSAT in SSAT pathway and the specific substrate in the N-acetylation regulation is L-dopa, partial metabolism of the substrate can generate an acetylated metabolite N-acetyl-L-dopa thought the introduction of SSAT, the SSAT upregulation is positively correlated with cancer, accordingly, L-dopa can be used as a cancer mark regulated by the SSAT upregulation, and a novel detection method is provided for early detection, early diagnosis, early treatment and the like of tumors. The method has the advantages of safely, efficiency, strong practicality, convenient and fast operating method, simple usage, high positive incidence, and remarkable effect, and can be used as an auxiliary means for preventing, diagnosing, detecting, protecting, treating and studying various tumors.
Owner:上海拜瑞曼克生物科技有限公司
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