284results about How to "Increase positive rate" patented technology

The invention discloses a probe set for detecting whole exons of extended genetic diseases. The probe set for detecting the whole exons of the extended genetic diseases comprises a standard whole exonprobe set, a whole genome copy number variation probe, and a mitochondrial loop full-length probe, and the genetic diseases comprise 6161 genetic diseases; the standard whole exon probe set can detect the genetic diseases caused by whole exon mutation, the genetic diseases comprise nervous system diseases, metabolic system diseases, endocrine system diseases, digestive system diseases, skeletal system diseases, urinary system diseases, immune system diseases, cardiovascular system diseases, blood system diseases, integument system diseases, ophthalmic system diseases, ear system diseases, respiratory system diseases, and genital system diseases; and the density of the mitochondrial probe is 6X; test samples comprise blood, fresh tissue, FFPE samples, and saliva. The invention discloses using method and kit and application of the probe set for detecting the whole exons of the extended genetic diseases.

The invention relates to a novel molecular cloning method, and in particular to a method for obtaining recombinant vectors by modifying initial vectors through combined utilization of a CRISPR/Cas9 system and a saccharomyces cerevisiae cell endogenous gene homologous recombination system; only through one-time transformation and selection operations, the method can simultaneously complete insertion of ore or more target DNA fragments into the initial vectors, deletion of one or more DNA fragments from the initial vectors and/or substitution of one or more DNA fragments on the vectors for one or more target DNA fragments. The invention further relates to a reagent kit for conveniently and effectively applying the method provided by the invention.

The invention discloses a method for efficiently separating single antigen-specific B lymphocyte from spleen cells. High-throughput rapid screening of single antigen-specific B lymphocyte is realizedby negative screening adopting multiple lymphocyte surface marker antibodies, establishment of a rapid fluorescence marked antigen substance method and high-specificity screening by use of a fluorescence marked antigen substance, and the positive rate of the finally obtained antibody-specific B lymphocyte is increased from less than 5% in a traditional hybridoma method to 30%. Therefore, the scheme can shorten monoclonal antibody development time to a great extent and greatly increase the number and diversity of obtained monoclonal antibodies.

The present invention provides a microbial sample processing device, comprising: a machine body provided with a machine chamber and a sealed movable door; and a sealed housing, a stretchable airbag, a sample tray, a mechanical arm, a bar code scanner, a liquefier, culture dishes, a gas-temperature sensor, and a temperature control device which are arranged in the machine chamber, wherein the sample tray is provided with a plurality of sample seats, the sample seats are used for placing the culture dishes, the liquefier is communicated with the culture dishes, each culture dish is provided with culture plates and a streak-inoculation pen used for streak inoculating the sample in the culture dishes from the liquefier on the culture plate. The microbial sample processing device is reasonable in structure, fast and safe, reliable and efficient, can complete the whole process of mixing and liquefying, streak inoculation, dilution and separation, and incubation of microbial community automatically in a one-stop manner, and can replace manual labor and ensure biological safety.

The invention discloses a fungus detection fluorescent dyeing liquid and a use thereof. The fungus detection fluorescent dyeing liquid comprises an independent dyeing liquid A and an independent re-dyeing liquid B. The dyeing liquid A comprises, by weight, 0.02-0.043% of a fluorescent brightener, 1-10% of potassium hydroxide, 10-20% of dimethyl sulfoxide, 0-5% of glycerin and 65-87% of water. The re-dyeing liquid B comprises, by weight, 0.1-0.5% of Evans blue and 99.50-99.90% of water. The invention discloses a use of the fungus detection fluorescent dyeing liquid. The invention provides a novel fast fungus infection detection product. The product can be used for detecting various fungi which may exist in a fresh or frozen clinical sample and paraffin and ethylene glycol methacrylate-coated tissue.

The invention provides somatic mutation sites of pathogenic genes of the thyroid cancer and application thereof, specifically to a group of mutation sites of the pathogenic genes of the thyroid cancer. The mutation sites are composed of a GNAS gene mutation site, a NRAS gene mutation site, a TSHR gene mutation site, etc. The gene mutation sites provided by the invention can be used as markers for identification of benign and malignant thyroid nodules.

The invention relates to a method and a kit for detecting cervical carcinoma by using PAX1 and CDH1 gene methylation. Particularly, the invention provides a kit, which contains (a) a primer pair aiming at methylation specificities and non-methylation specificities of a POX1 gene and a CDH1 gene, or contains (b) a methylation sensitivity restriction endonuclease, and a primer pair aiming at the specificities of the POX1 gene and the CDH1 gene. The kit can effectively separate the cervical carcinoma from cervicitis and HPV infection.

The invention provides use of a composition for preparing a kit for detecting cell proliferative abnormalities of an individual. The composition comprises a nucleic acid for detecting the methylation level of Septin9 and RNF180 genes as well as at least one target region in the fragments of the genes. Therefore, existence of cell proliferative abnormalities is indicated by virtue of integrating the methylation detection results of Septin9 and RNF180.

The invention discloses a method for enriching and purifying blood platelets. The method comprises the following steps: adding an anticoagulant into whole blood and centrifuging at the speed of 1000rpm for 10 minutes; absorbing one part above a red blood cell layer and adding normal saline into the residual red blood cell layer; centrifuging at the speed of 1000rpm for 10 minutes; taking one part above the red blood cell layer and mixing to obtain an enrichment C; adding a red blood cell lysis solution into the enrichment C; uniformly mixing and standing for half an hour; centrifuging at the speed of 1500rpm for 20 minutes; collecting liquid supernatant A and a middle white flocculent layer A; centrifuging the liquid supernatant A at the speed of 1500rpm for 10 minutes; collecting a bottom white layer B and mixing the bottom white layer B with the middle white flocculent layer A; adding the normal saline and centrifuging at the speed of 1500rpm for 10 minutes; and collecting a middle white layer C, namely the concentrated and enriched blood platelets. According to the method for enriching and purifying the blood platelets, a purification method for removing blood serum and breaking red blood cells on the basis of centrifuging and enriching is added, so that the red blood cells can be completely removed; and the enriching purity of the red blood cells is effectively improved and the pollution of white blood cells and the red blood cells is alleviated.

The invention discloses a kit for detecting Ditylenchus destructor based on loop-mediated isothermal amplification and application thereof. The invention provides a special primer for assistant identification of Ditylenchus destructor and consists of DNAs shown in sequences 1-14 in a sequence table. The kit provided by the invention comprises the special primer. According to the invention, the kit for assistant identification of Ditylenchus destructor is provided through designing the specific primer and optimizing reaction conditions; and through applying the kit, an interspecies level and an infraspecies level of detection can be achieved, and the problems that the routine molecule detection method has the defects of high cost, low efficiency and slow speed and can not meet the requirements of current plant quarantine and plant protection are solved.

The invention relates to a tuberculosis antibody multiple antigen ELISA detection kit and a preparation method thereof, which pertains to the field of tuberculosis medical immunology diagnostic techniques and mainly uses detection antigen, enzyme-linked antihuman IgG antibodies, substrates, positive control serum of tuberculosis patients, control serum of normal person, calf serum and polystyrene microplates to form the kit, wherein, the detection antigen adopts the mycobacterium tuberculosis complex strains of lipid Arabian mannose (LAM), 38kD and 16kD to be combined with arbitrary one or more than one mycobacterium tuberculosis recombinant proteins in the recombinant proteins of MPT63, MTB48 and CFP10-ESAT6. The mycobacterium tuberculosis has high sensitivity, strong specificity and complementarity, can be used for detecting specific antitubercular antibodies in such body fluid samples as serum, hydrothorax and the like, and assisting the diagnosis and differential diagnosis of tuberculosis.

The invention belongs to the technical field of gene editing, and particularly relates to a Zymomonas mobilis genome editing method based on a CRISPR-Cas12a system and application of the method. The method for editing a Zymomonas mobilis genome is established on the basis of the CRISPR-Cas12a system with Zymomonas mobilis (ZM4) as the modulus strain, the oriented editing of the genome is realized,a gene editing tool is provided for developing the rational design of an allogenic metabolic pathway and cell factory for producing biomass fuel and biological materials in the strain, and the development in metabolic engineering and related research fields is promoted. The method is technically characterized by including the steps of establishing an inducible expression Cas12a recombinant strainand an editing plasmid containing an artificial CRISPA expression unit, designing guiding RNA, connecting the guiding RNA to the editing plasmid after annealing the primer sequence of the guiding RNA, and shifting a target plasmid into a competent cell for expression editing.

The invention relates to a plasmid vector and a method for building a plant population by using the plasmid vector. The plasmid vector contains, at a replication origin, a gene expression box for expressing Cas9 protein, a first promoter, nucleotide sequences containing suicide gene sequences, a gRNA scaffold element and a termination signal, and at least one first enzyme cutting site located on the nucleotide sequence 5' end containing the suicide gene sequence and at least one second enzyme cutting site located at the nucleotide sequence 3' end containing the suicide gene sequence; furthermore, no first enzyme cutting site and no second enzyme cutting site exist in other positions of the plasmid vector. The first promoter is located at the upstream of the termination signal; the nucleotide sequences containing suicide gene sequences and the gRNA scaffold element are all located between the first promoter and the termination signal.

The invention provides real-time fluorescence quantitative PCR (Polymerase Chain Reaction) primers and probe for detecting campylobacter jejuni and a method for carrying out quantitative detection by using the primers and probe. The nucleotide sequences of the primers and probe are respectively shown in SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention also provides a kit for detecting campylobacter jejuni. The detection method and the kit thereof provided by the invention have the advantages of accurate detection, high sensitivity, strong specificity, simplicity, convenience, quicknessand good clinical specimen detection capability.

The invention provides a transformation method utilizing a red fluorescent protein as a selection marker. The method comprises the following steps of: synthesizing a fluorescent protein (FP) gene and modifying according to the bias of a rice codon, wherein the modified FP gene is used as a selective marker gene of rice transformation and plant regeneration; driving the FP gene by a callus/seed coat-specific promoter, closely connecting with a target gene, and transferring to rice embryonic calli under the mediation of Agrobacterium tumefaciens; screening for three times by a fluorescence microscope in 30 days after coculture; and further verifying an obtained transgenic seedling by utilizing a polymerase chain reaction (PCR) and Southern hybridization. The result shows that the positive rate of the transgenic seedling is up to 80 percent, which proves that FP serving as the selection marker of plant transformation is completely feasible. By the method, the potential hazard of the traditional selection markers such as antibiotic and herbicide resistance genes and the like to environment and food safety is effectively reduced. The invention provides a novel safe plant genetic transformation screening system.

The invention discloses a novel aerosol and droplet coronavirus pathogen collection mask and a collection method. The mask comprises ear hooks, a mask body and a breather valve on the mask body, the breather valve comprises an antifouling layer, a filtering layer, a heat preservation layer, an antibacterial virus layer and a skin-friendly layer, and the breather valve further comprises a pathogencollecting layer which sequentially comprises the antifouling layer, the filtering layer, the antibacterial virus layer, the heat preservation layer, the pathogen collecting layer and the skin-friendly layer from outside to inside. The invention realizes a new sampling mode which is convenient and simple to use and can realize aerosol and droplet pathogens and even sputum, and is beneficial to saving medical resources and improving the positive rate of samples. A breather valve of an existing KN95 mask is modified into a detachable valve with filtering and enriching functions, and large-scaleproduction of the breather valve can be achieved. Production difficulty is low, and the minvention is simple and easy to implement.

The invention discloses a liquid injection and suction method for a cell slide-making dyeing machine. The liquid injection and suction method comprises the following steps of 1) injecting a proper amount of a body fluid sample, which is required for detection, in a sampling bottle onto a glass slide through a liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of a round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid injection pipe during liquid injection; 2) sucking away redundant waste liquid through a liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall, and tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe; 3) injecting dyeing liquid required for dyeing through the liquid injection pipe arranged on the cell slide-making dyeing machine in a manner of approaching one side of the round pipe wall, stewing the dyeing liquid, and titling the glass slide towards one side of the round pipe wall approaching the liquid injection pipe; 4) sucking away the redundant waste liquid through the liquid suction pipe arranged on the cell slide-making dyeing machine in a manner of approaching the bottom of one side of the round pipe wall after cells are colored, tilting the glass slide towards one side of the round pipe wall approaching the liquid suction pipe when the redundant waste liquid is sucked away, and repeating the step 3) and the step 4) for 2-8 times until the dyeing operation is finished.

The invention relates to a method for quickly obtaining a capsicum transgenic plant, which comprises the following steps: sampling; activating and infecting agrobacterium; preparing a regeneration culture medium and a screening culture medium; inoculating the immature embryo, and culturing; and forming adventitious bud and regenerating the plant, thereby efficiently obtaining the capsicum transgenic regenerated plant. In the invention, it usually takes 7 weeks from the immature embryo infected by agrobacterium to the acquisition of the regenerated plant, the formation rate of the regenerated plant in the culture process is up to 50% to the maximum, and the positive rate of the transgenic plant is up to 80%. In the invention, the immature embryo is used as a receptor material to infect theagrobacterium and carry out inoculation and culture so as to directly form the regenerated plant, thereby shortening the culture period and enhancing the culture effect and genetic transformation efficiency. If applied to genetic functional verification and breeding practice, the invention can greatly accelerate the research into capsicum functional genomics and the development of breeding practice.

The invention belongs to the field of biotechnical detection, and particularly relates to a bovine, goat, porcine and canine brucella typing fluorescent PCR (polymerase chain reaction) detection reagent kit and preparation and application thereof. The bovine, goat, porcine and canine brucella typing fluorescent PCR reagent kit contains bovine brucella detection primers and probes, goat brucella detection primers and probes, porcine brucella detection primers and probes and canine brucella detection primers and probes. The bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit, the preparation and the application have the advantages that the bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit is high in detection sensitivity, the lowest detection limit is 1*10<3> copy/ml, and the accuracy and the positive rate can reach 100%.

The invention provides a genetic marker, real-time fluorescence quantitative PCR (polymerase chain reaction) primers and a probe used for detecting peste des petits ruminants through the N gene sequencing and comparison of the peste des petits ruminants, and the nucleotide sequences of the genetic marker, the primers and the probe are respectively disclosed in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention also provides a quantitive detection method and a detection kit for the peste des petits ruminants virus. The detection method and the detection kit disclosed by the invention have the advantages of accuracy in detection, high flexibility, strong specificity, easiness and rapidness, and the specimen detection capacity is good.

The invention, relating to the technical field of medicine, relates to a method for detecting the activity of spermidine/spermine N1-acetyltransferase, that is, a quantitative technique of analyzing SSAT activity through detecting acetylated metabolites of the SSAT substrate. According to the invention, the N-acetylation is regulated mainly through or only through SSAT in SSAT pathway and the specific substrate in the N-acetylation regulation is L-dopa, partial metabolism of the substrate can generate an acetylated metabolite N-acetyl-L-dopa thought the introduction of SSAT, the SSAT upregulation is positively correlated with cancer, accordingly, L-dopa can be used as a cancer mark regulated by the SSAT upregulation, and a novel detection method is provided for early detection, early diagnosis, early treatment and the like of tumors. The method has the advantages of safely, efficiency, strong practicality, convenient and fast operating method, simple usage, high positive incidence, and remarkable effect, and can be used as an auxiliary means for preventing, diagnosing, detecting, protecting, treating and studying various tumors.