190results about How to "Simplify testing procedures" patented technology

The invention belongs to the technical field of non-destructive testing and measuring, and discloses a focusing rotary scanning photoacoustic ultrasonic blood vessel endoscope imaging device and a focusing rotary scanning photoacoustic ultrasonic blood vessel endoscope imaging method. The device comprises a photoacoustic ultrasonic endoscope imaging probe, a rotating connecting part and a peripheral circuit part, wherein pulse laser generates 90-degree reflection at the light outlet end and is irradiated on the blood vessel wall after being gathered by a cylindrical surface light gathering lens, photoacoustic signals are generated, a sound-sensitive element receives the photoacoustic signals, the photoacoustic signals are collected and recorded after being converted, the synchronous working of a data collector and a pulse laser is realized, triggering signals generated by the pulse laser trigger an ultrasonic pulse emitting and receiving device to emit electric signals, the electric signals trigger the sound-sensitive element to emit ultrasonic signals, the ultrasonic signals are reflected after reaching the blood vessels, are received by sound-sensitive elements and are collected and recorded after being converted, a step motor drives the device for carrying out scanning to obtain the whole blood vessel fault data, and photoacoustic and ultrasonic images are obtained after the processing. The sound-sensitive elements are shared by ultrasonic and photoacoustic images, and the high-resolution and high-sensitivity blood vessel internal ultrasonic photoacoustic imaging can be realized.

The invention discloses an urban gas buried pipeline leakage detection and locating method and applications thereof. Based on an acoustic emission technology and combined with the current gas pipeline laying situation of Changzhou city, layout schemes and relevant parameters of sensors are designed, and the leakage detection method under the situation of guaranteeing normal gas transmission of buried pipelines and not excavating pipelines is determined. Simultaneously, wavelet noise reduction and relevant analysis methods are introduced, and accordingly, the locating accuracy is improved, and objective and credible results are obtained. According to the urban gas buried pipeline leakage detection and locating method and applications thereof, the application of the acoustic emission detection technology in gas buried pipeline leakage detection is facilitated and improved to a large extent, and hence, while the method is effective and accurate in detection and cost saving, popularization and use values are increased, and the method has great significances in enhancement of urban gas buried pipeline risk control.

The invention relates to an integrated opto-acoustic, ultrasonic and opto-acoustic elastic endoscopic imaging device which is characterized in that the imaging device comprises a pulse laser, an optical filter, a diaphragm, an objective and an optical fiber coupler sequentially arranged along the transmission path of pulse laser and also comprises a multimode optical fiber, an optical fiber slip ring, a rotation component, a rotary step motor, an electric slip ring, an integrated probe, a translation platform, a translation step motor, an ultrasonic pulse transceiver, a time delay unit and a computer, wherein the computer is in signal connection with the pulse laser; the pulse laser is in signal connection with the time delay unit; the time delay unit is in signal connection with the ultrasonic pulse transceiver; and the ultrasonic pulse transceiver is in signal connection with the electric slip ring. The invention also relates to a method for imaging by using the imaging device. In the invention, three kinds of imaging can be obtained, the detection precision can be improved while multi-parameter and multi-scale information in blood vessels can be obtained, and the invention belongs to the technical field of blood vessel endoscopy.

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

The invention discloses an ISSR molecular marking based method for constructing a Chinese asparagus bean generic resource database and application thereof. The invention also utilizes the data base to identify the trueness of a variety; according to field forms, the consistency and typicality among individual plants of the variety are identified, separated and atypical materials are given up, and DNA of the individual plant of the consistent and typical variety is picked up; by utilizing three ISSR markings with high polymorphism, the consistency of DNA level among the individual plants is primarily selected, and the variety and individual plant constructing a fingerprint are finally determined; and by utilizing the finally selected variety and individual plant, the ISSR analysis of the Chinese asparagus bean is made to construct a fingerprint database. The invention overcomes the defects existing in the construction of the prior generic resource database, such as separation among different individual plants of the variety and impurity on the DNA level, the reliability and accuracy of the Chinese asparagus bean ISSR fingerprint data base constructed by the method are greatly improved, and the planting varieties of the Chinese asparagus bean can be effectively and conveniently identified and distinguished.

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus plantarum in probiotic dairy products. The method comprises the following steps: aiming at the lactobacillus plantarum in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the lactobacillus plantarum, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring lactobacillus plantarum in probiotic dairy products.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention relates to a detection method of degree of damage of plasma, which comprises the following steps: providing a batch of chips; providing the upper limit value of the threshold voltage shift of the batch of chips; testing the drain current of the batch of chips to obtain the distribution of the drain current; extracting part of chips from the batch of chips, testing the threshold voltage of the part of chips to obtain the threshold voltage shift and distribution thereof; determining the qualification rate of the part of chips according to the threshold voltage shift and the upper limit value; applying the qualification rate of the part of chips to the batch of chips to establish the drain current standard value of the batch of chips; and detecting the degree of damage of plasma of the batch of chips according to the drain current standard value of the batch of chips. The method can lower the detection cost, enhance the detection efficiency and realize on-line measurement without damaging the chips.

The invention discloses a liberobacter asiaticum detection method based on visible light images. In the training stage, a large number of images of citrus leaves infected with liberobacter asiaticum and images of normal leaves are collected, the feature values of the texture feature and the color feature are extracted, the above feature values and the feature values of normal leaves are trained and learned through a BP neural network, and the optimal BP neural network model is obtained; in the identification stage, the features of the leaf images to be identified are extracted and input into the optimal BP neural network model, and then whether a citrus tree is healthy is judged. By means of the liberobacter asiaticum detection method based on visible light images, the type of the liberobacter asiaticum and whether the citrus tree is not yellowed due to liberobacter asiaticum are further judged, early, accurate and non-destructive diagnosis can be carried out on the liberobacter asiaticum, and the liberobacter asiaticum detection method based on visible light images has the advantage of being high in detection accuracy.

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products. The method comprises the following steps: aiming at the Lactobacillus casei in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the Lactobacillus casei, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products.

The invention belongs to the detection technical fields of feed science and feed additives, and more specifically relates to a rapid qualification and quantitation measurement method of saccharomyces cerevisiae in an additive premix sample. According the invention, cDNA gradient dilution is taken as an external standard substance used for distinguishing dead bacteria and live bacteria for accurately detecting the amount of the live bacteria in a sample to be measured. The invention is capable of measuring saccharomyces cerevisiae in premix sample with simple, rapid and accurate qualification and quantitation without pure culture of saacharomyces cereisiae, the whole process has the advantages of simple detection process, high detection efficiency, high accuracy and short detection period; and the invention provides a guarantee a long term development for a microecological preparation industry.

The invention provides a RPA (recombinase polymerase amplification) kit used for detecting Ebola virus, special-purpose primers and probes of the RPA kit, and applications of the RPA kit in detection of Ebola virus. The RPA kit, the special-purpose primers, and the probes are designed based on Ebola virus NP gene conserved sequence, and the Ebola virus NP gene conserved sequence possesses an oligonucleotide sequence represented by SEQ ID NO.1. It is shown by research results that the RPA kit can be used for rapid detecting of Ebola virus in samples with specificity and sensitivity.

The invention belongs to the technical field of bioengineering, and in particular relates to a method for quantitatively analyzing clostridia, lactobacilli, bacilli and methanobacteria in pit mud. The technical problem to be solved by the invention is to provide a new choice for analyzing the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud. The technical scheme of the invention is as follows: the method for quantitatively analyzing the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud comprises the steps of a, preparation of a standard curve; b, extraction of genome DNA; c, amplification; d, quantitative analysis. The method disclosed by the invention can be used for analyzing the change trend of the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud.

The invention provides a genetic marker for detecting canine parvovirus, and the genetic marker contains a nucleotide sequence as shown in SEQ ID NO.1. The invention also provides specific primers and a probe for detecting the genetic marker, a method for quantitative detection on the canine parvovirus and a detection kit. The method and the kit, provided by the invention, have the advantages of high sensitivity, strong specificity and easiness in operation, can meet the requirement of rapid detection on sick animals or products thereof, and has the significance for guaranteeing healthy development of the domestic animal husbandry and preventing the canine parvovirus from spreading, and meanwhile provides a new technological means for study on pathogenicity of the canine parvovirus in a natural reservoir.

A testing device for testing computer peripheral devices with Universal Serial Bus (USB) is provided. The testing device includes a CPU, a memory with a predetermined firmware program inside, which is connected to the CPU, a control chip with USB interface, which is connected to the CPU and is provided with a plurality of connecting ports for connecting with peripheral devices to be tested. The firmware program inside the memory is used for controlling the CPU to test the peripheral devices to be tested through the control chipset. According to one CPU and each peripheral device connected through by a control chip with USB interface for processing test procedures only, the hardware could be more simpler and efficiency.

The invention relates to a method for detecting ractopamine content in food. The method is characterized by comprising the following steps: preparing a capture probe; preparing a signal probe, treating a sample to be detected, and detecting by using a glucometer. Compared with the prior art, the method has the advantages that the signal is converted into a glucose signal which can be detected by the glucometer through an enzymatic effect, field detection of a nonsugar glucometer is realized, the stability is high, the detection limit is low, the detection range is wide. Moreover, the detection cost is low, the detection program is simple, the detection effect is good, particularly general staff can perform field detection, and the strict requirement that the professional staff need to operate in a lab in the prior art is avoided.

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus acidophilus in probiotic dairy products. The method comprises the following steps: aiming at the Lactobacillus acidophilus in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the Lactobacillus acidophilus, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring Lactobacillus acidophilus in probiotic dairy products.