26 results about "Blood type" patented technology

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

Blood Group and Glucose Detection Device

The present disclosure provides methods and kits for detecting Group A Streptococcus in biological samples. More particularly, the present disclosure provides methods for enhancing the specificity and sensitivity of Group A Streptococcus immunoassays by including N-propionyl-D-glucosamine, 2-N-butanoyl-D-glucosamide, Bis-(2-(D-2-deoxy-glucosaminyl))-PEG3-amide, m-PEG4-glucosamine, m-PEG6-glucosamine, or m-PEG10-glucosamine. The methods and kits disclosed herein are thus useful for reliable and early diagnosis of streptococcal infections in a subject.

The present invention relates to humanized anti-Lewis Y antibodies that specifically bind Lewis Y and do not show any cross-reactivity. In particular, the humanized anti-Lewis Y antibodies do not bind to Lewis b or any other blood group carbohydrate antigen. In particular, the present invention relates to humanized anti-Lewis Y antibodies for use in the treatment of cancer.

The invention provides a puncture device and a blood type analyser, the puncture device comprises a grabbing mechanism, an elastic mechanism and a puncture piece, the elastic mechanism comprises an elastic piece with a first pop-up part and a second pop-up part, the puncture piece is internally provided with a moving channel, the first pop-up part of the elastic piece can extend to the position below the moving channel, and the second pop-up part of the elastic piece can extend to the position below the moving channel; a second pop-up part of the elastic part can abut against an abutting part in the moving channel to assist separation of the blood card and the puncture needle, so that the probability that the puncture part is stuck in the separation process of the puncture part and the grabbing mechanism is reduced, taking, placing and replacing of the puncture part are smoother, and the work efficiency is improved. The elastic piece does not need to be installed on the puncture piece, the installation space of the elastic piece does not need to be reserved in the puncture piece, and the size of the puncture block can be reduced.

This invention relates to the fields of molecular diagnostics and gene sequencing technology, specifically to a complete solution for high-precision genotyping of the human Lewis blood group system. The invention provides primer pairs for specifically amplifying long fragments of the Lewis blood group coding genes FUT2 and FUT3, and an optimized single amplification system; a library construction method adapted for nanopore sequencing; and a Lewis (FUT2, FUT3) genotyping method based on long-read data for haplotype analysis. This invention overcomes the detection limitations of conventional techniques, simultaneously acquiring the complete sequence and phase information of key regions of the FUT2 and FUT3 genes through a single amplification system, achieving accurate genotyping of common Lewis blood group phenotypes (Le(a+b−), Le(a−b+), Le(a−b−)) and effective identification of rare variants such as weakly expressed and deleted variants.

The utility model belongs to the technical field of red blood cell storage devices, and particularly relates to a red blood cell cold stock taking storage material box which comprises a storage material box body, a storage structure is arranged in the storage material box body and comprises a classification mechanism and an auxiliary mechanism, and the classification mechanism comprises a bearing plate, a guide rod, a classification plate, a first telescopic rod and a clamping plate. And one side of the bearing plate is connected with one end of a guide rod. According to the erythrocyte cold stock taking and storing box, stockers can be quickly and conveniently clamped and fixed, the stockers of erythrocytes of different blood types can be quickly and conveniently stored in a classified mode, and meanwhile the distance between a plurality of classification plates can be quickly and conveniently adjusted; according to the red blood cell storage box, different sizes of storage devices can be better clamped and fixed, the problem that red blood cells of different blood types cannot be quickly and conveniently classified and stored, and consequently re-distinguishing is needed when the red blood cells are taken is solved, and the working efficiency of the storage box is improved.

The present application relates to the field of medical detection, and in particular, the present application relates to a kind of integrated genotyping method of multiple blood cell antigens related to selective blood transfusion and application thereof.The genotyping method described in the present application is an integrated typing method based on DNA sequence related to multiple red blood cell, platelet and white blood cell antigen coding genes related to blood transfusion, which can reduce the incidence of xenoantigen sensitization caused by alloimmunization, promote precision blood transfusion, and cover blood group genes including ABO, RhD, RhCE, HPA (ITGB3, GP1BA, GP1BB, ITGA2B, ITGA2, CD109, GP9), HLA-A, -B, -C, -DRB1, -DQB1, ABO, FUT1, FUT2, etc.The covered alleles can reach tens of thousands.Moreover, after the platform built in the present application, second-generation sequencing library construction and third-generation sequencing library construction can be carried out, which provides an excellent re-creation and reproduction platform for future haplotype confirmation of multiple site variations in blood group genes, and has important significance for future precision blood transfusion.

The invention relates to a breeding method of a fluoride-resistant and polyhedrosis-resistant bombyx mori strain, and belongs to the technical field of bombyx mori genetic breeding. In order to solve the technical problems that an existing silkworm variety cannot resist fluoride poisoning and pyosis at the same time, and resistance is prone to loss, the method comprises the following steps: hybridizing a germplasm carrying a fluorine-resistant major gene and a germplasm carrying an antiviral dominant major gene to obtain an F1 generation, and backcrossing the F1 generation with a fluorine-resistant parent after fluorine-resistant screening to obtain a BC1 generation; and finally, fluorine-resistant and antiviral characters are synchronously screened and improved through selfing generations, and directional breeding is carried out by combining whole-course recorded development and cocoon quality characters. According to the method, a new silkworm strain with double resistance is successfully bred, and the method is mainly used for solving the problem of low survival rate of silkworm breeding in a fluorine pollution area and a virus high incidence area in industrial production.

The invention discloses a method for accurately detecting fetal blood types based on a pregnant woman peripheral blood cff-DNA enrichment method, and belongs to the technical field of biomedical detection. According to the method, an acoustically responsive conformation lock aptamer probe is used, and the probe comprises a recognition domain for specifically recognizing placenta-derived nucleosome and a capture group shielded by an intramolecular conformation lock space. The conformational lock can be selectively opened by a resonant sound field of a specific frequency. In the implementation of the method, the probe in a locked state is mixed with a plasma sample, and then the mixture is processed in the multistage acoustic flow control micro-fluidic chip; and finally, capturing the compound in the activated state through magnetic beads, and eluting to obtain high-purity fetal nucleosome for subsequent DNA extraction and detection. According to the method, efficient, rapid and automatic enrichment of the target object is realized through innovative sequential control of purification and activation in sequence, and the purity and the yield are remarkably improved.

The invention discloses an affinity chromatography medium for removing an anti-A / anti-B antibody and a preparation method and application of the affinity chromatography medium, the general formula of the chromatography medium is shown in the formula I. M is a matrix, A comprises 2-15 carbon atoms and further comprises one or more hydroxyl groups and / or-NH-,-O-or-S-, S is a straight-chain or branched-chain alkyl group with 1-17 carbon atoms, and the formula I is shown in the description. D is a straight chain or branched chain alkyl group with 1-17 carbon atoms, n is 1 or 2, and T is a blood type saccharide ligand. The chromatography medium obtained in the invention has good stability and binding capacity.

The application discloses a kind of based on centrifugal microfluidic chip's full-automatic blood type analyzer, belong to blood type analysis equipment technical field.The analyzer includes bottom plate, and sample reagent bin module is arranged on bottom plate, chip processing module, new chip storage bin module, chip recycling bin module, sample adding arm module and chip pusher module;Chip pusher module is used to contact and promote microfluidic chip from new chip storage bin module translation to chip processing module processing, and promote after microfluidic chip translation to chip recycling bin module recycling after chip processing module processing;Sample adding arm module is located at the top of sample reagent bin module and chip processing module, for adding sample or reagent.The application is based on centrifugal microfluidic chip, realizes full-automatic sample and reagent addition, chip transfer, chip loading, chip centrifugation, result detection judgment and chip recycling, without manual intervention, support sample no shutdown sample, sample into result, smaller volume under same test speed.

The present application relates to a kind of primer set and kit for Sanger sequencing method for human RHD blood group genotyping detection, belong to biomedical clinical molecular detection field.The primer set for Sanger sequencing method for human RHD blood group genotyping detection of the present application, including specific PCR amplification primer set, the specific PCR amplification primer set includes 9 pairs of amplification primer designed according to RHD gene specific sequence;The primer set also includes 10 RHD specific sequencing primers corresponding to amplification product.The present application has the following technical effects: the present application adopts the RHD gene specific amplification primer designed by ARMS and homologous sequence specific base method, and specificity and accuracy are higher;With the RHD specific sequencing primer of the present application, the gene sequence of the sample to be detected is detected by Sanger sequencing method, RHD genotype is interpreted, and new mutation point can be found.The identification result of RHD blood group gene 1 to 10 exons can be obtained by the kit of the present application, so as to accurately determine the RHD gene typing of experimental sample.

Disclosed herein are compositions and methods relating to genetically engineered mammalian cells that include modulating the expression of selected genes. The genetically engineered mammalian cells described herein advantageously have improved viability. In some embodiments, the mammalian cells are genetically engineered to reduce or eliminate expression of a renalase gene compared to the expression level of the same cell type that is not genetically engineered, and the cells are further genetically engineered to reduce or eliminate expression of an ABO gene.

This invention discloses a composite material based on hollow mesoporous carbon spheres loaded with ABO blood group monoclonal antibodies, comprising hollow mesoporous carbon spheres and ABO blood group monoclonal antibodies loaded on the hollow mesoporous carbon spheres, wherein the ABO blood group monoclonal antibodies are selected from at least one of type A antibodies and type B antibodies. This invention also discloses a method for preparing the composite material, comprising: modifying the surface of the hollow mesoporous carbon spheres with amino groups; dispersing the spheres in an ABO blood group monoclonal antibody solution; and stirring the reaction. This invention immobilizes ABO blood group monoclonal antibodies on the surface of hollow mesoporous carbon spheres, improving the stability, specificity, and sensitivity of the antibodies, and achieving rapid and accurate ABO blood group identification. This invention also discloses a kit comprising the above-mentioned composite material, and the application of this composite material in ABO blood group detection.

This invention discloses the application of 6-benzylaminopurine and drugs containing 6-benzylaminopurine in the prevention and / or treatment of hemorrhagic septicemia in silkworms. Using 6-benzylaminopurine as the core active ingredient, drugs for the prevention and treatment of hemorrhagic septicemia in silkworms can be prepared. Experiments show that when the final concentration of 6-benzylaminopurine reaches 50 μM, it can significantly inhibit the replication of BmNPV, and can still exert an inhibitory effect after the virus has completed cell infection, demonstrating good therapeutic efficacy. This invention provides a new solution for the research and development and production of drugs for hemorrhagic septicemia in silkworms, and has broad application prospects and promotional value.

This invention relates to the fields of molecular diagnostics and gene sequencing technology, specifically to a primer set, amplification system, amplification method, library construction method, and sequencing method for Kidd blood group system genotyping amplification based on long-read nanopore sequencing. This invention provides primers and an optimized amplification system for specifically amplifying long fragments of the Kidd blood group encoding gene SLC14A1; a library construction method adapted for nanopore sequencing; and a SLC14A1 genotyping method based on long-read data for haplotype analysis. This invention overcomes the limitations of conventional techniques that only detect single SNPs, enabling the simultaneous acquisition of the complete sequence and phase information of key regions of the SLC14A1 gene, achieving accurate genotyping of JKA / JKB and effective identification of rare variants such as weakly expressed and deleted variants. This invention provides a powerful molecular tool for clinical transfusion safety assurance, rare blood group bank construction, and genetic research.

The present application provides a multiplex assay for identifying a subject's ABO histo-blood subtype antibody profile, the multiplex assay comprising incubating a biological sample from the subject with a bead composition containing Luminex® beads individually linked to type I, type II, type III, and type IV ABO-A and ABO-B subtype glycan antigens. Each of the antigen-linked beads contains bovine serum albumin (BSA)-linked ABO subtype antigens bound to the corresponding bead, and the amount of BSA-linked ABO subtype antigen is 1.0 x 10 6 Approximately 5 μg per bead. Compositions and kits for performing multiplex assays are also provided.

A first aspect of the invention is directed to a process for the preparation of cellular blood components with reduced expression of antigen(s) (blood groups) compared to the native state, comprising: (a) Providing a solid carrier carrying at least one enzyme; (b) Providing a liquid phase LP1 comprising at least one cellular blood component having antigen(s) with a native expression of antigen(s) LA(1); and (c) Contacting the solid carrier carrying at least one enzyme provided according to step (a) with the liquid phase provided according to step (b) under conditions allowing a removal of at least a part of the antigen(s) from the at least one cellular blood component; thereby obtaining a liquid phase LP2 comprising the at least one cellular blood component having antigen(s) with a reduced expression of antigen(s) LA(2), wherein LA(2) is < LA(1), and the solid carrier carrying the at least one enzyme at a concentration CSC(1). In a second aspect, the invention relates to a system for removing antigen(s) from cellular blood components, and a third aspect is directed to specific α-1,3-galactosidases. A fourth aspect of the invention is related to a cellular blood component having a reduced expression of antigen(s) LA(2) compared to a native expression of antigen(s) LA(1) of said cellular blood component with LA(2) < LA(1), obtained or obtainable by a process according to the first aspect of the invention, wherein a fifth aspect of the invention is directed to the cellular blood component with LA(2) < LA(1) per se. In a sixth aspect, the invention relates to the cellular blood component having a reduced expression of antigen(s) LA(2) compared to a native expression of antigen(s) LA(1) of said cellular blood component with LA(2) < LA(1) of the fourth or the fifth aspect of the invention for use in transfusion.

The present invention is directed to providing a canine with protective immunity against Leptospira serovar Icterohaemorrhagiae with a vaccine that comprises non-Icterohaemorrhagiae Leptospira serovar.

The present application relates to a kind of human RHCE blood group genotyping primer group and kit based on Sanger sequencing, belong to biomedical clinical molecular detection field.The primer group of the present application includes 9 pairs of primers designed according to the specific sequence of RHCE gene;The 9 pairs of primers are used to amplify RHCE 1 to 10 exons.The primer group also includes 10 RHCE specific sequencing primers corresponding to amplification primer.The specific amplification primer designed by ARMS combined with homologous sequence specific base method is high in specificity, cooperates with the RHCE specific sequencing primer of the present application, detects the gene sequence of the sample to be detected by Sanger sequencing method, interprets the RHCE genotype, and new mutation point can be found.The identification result of RHCE blood group gene 1 to 10 exons can be obtained by the kit of the present application, so as to accurately determine the RHCE high-resolution genotyping of experimental sample.

The application discloses a canine leptospira, icterohaemorrhagia leptospira and canine coronavirus triple inactivated vaccine. The application is prepared by adding adjuvant after inactivation of the canine leptospira, icterohaemorrhagia leptospira and canine coronavirus epidemic strain separated in China. The application relates to a pathogen separation culture, identification, inactivation method, production process, vaccine storage and immunization method. The canine triple inactivated vaccine is used for preventing canine leptospira disease, icterohaemorrhagia leptospira disease and canine coronavirus disease, and high antibodies can be generated after immunization of the canine, and the high antibodies have a good protective effect on virulent attack. The leptospira is a zoonosis, the canine is a companion animal of human, and the pet canine is in close contact with human, so the triple inactivated vaccine has good social benefits and important public health significance.

The invention provides a spectrum cell composition as well as a preparation method and application thereof. The cell composition comprises the following blood group system antigens: D, C, c, E and e antigens of an Rh system; a Jka antigen and a Jkb antigen of a Kidd system; m, N, S, s and Mur antigens of an MNS system; an Fya antigen and an Fyb antigen of the Duffy system; a Dia antigen and a Dib antigen of a Diego system; a K, k antigen of the Kell system; a Lea antigen and a Leb antigen of the Lewis system; a P1 antigen of the P system; and Doa and Dob antigens of a Do system. The spectrum cell composition provided by the invention can more accurately identify the Mur antibody, and also considers detection of other common antibodies, so that hemolytic reaction caused by missing detection of the antibody of a blood transfusion patient is avoided, the problem of missing detection of the Mur antibody is effectively solved, and blood transfusion safety is better guaranteed.

The application discloses a sufficient blood drainage type blood transfusion device, which comprises disposable blood transfusion consumables, the main body of the disposable blood transfusion consumables is connected with a first hose, a second hose and a third hose at one end, the other end of the first hose and the second hose can be respectively connected with a physiological saline bottle and a blood bag through joints, the other end of the third hose can be pierced into a human body through a needle, and a white blood cell filter is arranged on the pipeline, a fourth hose is additionally arranged between the first hose and the third hose, and the connecting point of the fourth hose and the third hose is located between the white blood cell filter and the needle, so that the platelets can be fully utilized and the blood resources can be saved.