This invention relates to the fields of
molecular diagnostics and
gene sequencing technology, specifically to a primer set, amplification
system, amplification method,
library construction method, and sequencing method for Kidd blood
group system genotyping amplification based on long-read
nanopore sequencing. This invention provides primers and an optimized amplification
system for specifically amplifying long fragments of the Kidd blood group encoding
gene SLC14A1; a
library construction method adapted for
nanopore sequencing; and a SLC14A1
genotyping method based on long-read data for
haplotype analysis. This invention overcomes the limitations of conventional techniques that only detect single SNPs, enabling the simultaneous acquisition of the
complete sequence and phase information of key regions of the SLC14A1
gene, achieving accurate
genotyping of JKA / JKB and effective identification of rare variants such as weakly expressed and deleted variants. This invention provides a powerful molecular tool for clinical transfusion
safety assurance, rare blood group
bank construction, and genetic research.