39 results about "Blood type" patented technology

The invention discloses a pharmaceutical composition for preventing and treating chronic atrophic gastritis. The pharmaceutical composition is prepared from the following raw materials in the following weight ratio: 14-18 parts of peach kernel, 10-14 parts of Chuanxiong rhizome, 8-12 parts of Panax notoginseng, 6-10 parts of safflower, 20-28 parts of kelp, 10-14 parts of selfheal, 15-25 parts of codonopsis pilosula, 10-14 parts of atractylodes macrocephala, 12-16 parts of fructus aurantii, 8-12 parts of amomum villosum, 12-16 parts of bupleurum, 10-18 parts of citron, and 5-10 parts of fucoidan. The pharmaceutical composition is used to treat chronic atrophic gastritis of gastric collateral blood stasis type by promoting blood circulation and removing blood stasis, unblocking meridians and relieving pain, eliminating phlegm, softening and dispersing nodules, invigorating qi and promoting qi circulation, and soothing the liver and harmonizing the stomach. The invention also discloses a preparation method of the pharmaceutical composition.

The invention relates to a primer group for detecting P1PK and Lewis blood group genotyping, a kit and application, and relates to the technical field of biology. The primer group comprises 12 groups of primer groups, and the nucleotide sequences of the 12 groups of primer groups are as shown in SEQ ID NO: 1-36. The 12 primer groups respectively correspond to 12 detection sites of P1PK and Lewis blood group systems, and the established detection method can effectively detect mutation conditions of 12 SNP sites, so that genetic typing of two blood group systems is realized. Compared with the existing detection technology, the method has the advantages that multi-system and multi-site simultaneous detection can be realized, the detection efficiency and the reaction sensitivity are improved, the defects of complicated operation, tedious process and the like of the existing detection method are overcome, and the method has a remarkable clinical application value.

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

Blood Group and Glucose Detection Device

The present disclosure provides methods and kits for detecting Group A Streptococcus in biological samples. More particularly, the present disclosure provides methods for enhancing the specificity and sensitivity of Group A Streptococcus immunoassays by including N-propionyl-D-glucosamine, 2-N-butanoyl-D-glucosamide, Bis-(2-(D-2-deoxy-glucosaminyl))-PEG3-amide, m-PEG4-glucosamine, m-PEG6-glucosamine, or m-PEG10-glucosamine. The methods and kits disclosed herein are thus useful for reliable and early diagnosis of streptococcal infections in a subject.

The present invention relates to humanized anti-Lewis Y antibodies that specifically bind Lewis Y and do not show any cross-reactivity. In particular, the humanized anti-Lewis Y antibodies do not bind to Lewis b or any other blood group carbohydrate antigen. In particular, the present invention relates to humanized anti-Lewis Y antibodies for use in the treatment of cancer.

A method and a kit for genotyping of multi-system red blood cell (RBC) blood group based on next-generation sequencing (NGS) are provided. Multiplex PCR capture primer sets and amplification reaction conditions thereof can be designed using Illumina as a platform to simultaneously detect full-length coding regions and spliced flanking regions of 25 blood group genes in 17 RBC blood group systems among three reactions, thereby enabling rapid genotyping of the 17 RBC blood group systems and multiple key rare blood groups therein. The method and the kit are based on high-throughput sequencing and have a high detection efficiency and a low average cost; the method and the kit also break through a limitation of existing blood group gene detection technology that cannot distinguish haplotypes; moreover, variations including known mutation sites and unknown mutation sites can be detected to obtain comprehensive and accurate detection results.

The invention provides a puncture device and a blood type analyser, the puncture device comprises a grabbing mechanism, an elastic mechanism and a puncture piece, the elastic mechanism comprises an elastic piece with a first pop-up part and a second pop-up part, the puncture piece is internally provided with a moving channel, the first pop-up part of the elastic piece can extend to the position below the moving channel, and the second pop-up part of the elastic piece can extend to the position below the moving channel; a second pop-up part of the elastic part can abut against an abutting part in the moving channel to assist separation of the blood card and the puncture needle, so that the probability that the puncture part is stuck in the separation process of the puncture part and the grabbing mechanism is reduced, taking, placing and replacing of the puncture part are smoother, and the work efficiency is improved. The elastic piece does not need to be installed on the puncture piece, the installation space of the elastic piece does not need to be reserved in the puncture piece, and the size of the puncture block can be reduced.

This invention relates to the fields of molecular diagnostics and gene sequencing technology, specifically to a complete solution for high-precision genotyping of the human Lewis blood group system. The invention provides primer pairs for specifically amplifying long fragments of the Lewis blood group coding genes FUT2 and FUT3, and an optimized single amplification system; a library construction method adapted for nanopore sequencing; and a Lewis (FUT2, FUT3) genotyping method based on long-read data for haplotype analysis. This invention overcomes the detection limitations of conventional techniques, simultaneously acquiring the complete sequence and phase information of key regions of the FUT2 and FUT3 genes through a single amplification system, achieving accurate genotyping of common Lewis blood group phenotypes (Le(a+b−), Le(a−b+), Le(a−b−)) and effective identification of rare variants such as weakly expressed and deleted variants.

The present invention provides a chemoenzymatic synthesis method for blood group trisaccharide, which includes: dissolving a substrate, GDP-Fuc, and magnesium chloride in water, adjusting the pH of the solution, adding the enzyme α1,2-FucT, and stirring for reaction to obtain a first product reaction solution; adding a de-enzyme solvent, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the first product; adding a glycosylated product, adjusting the pH after dissolution, and adding a glycosyltransferase to obtain a second product reaction solution; using a de-enzyme solvent to remove the glycosyltransferase, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the second product; separating and purifying the aqueous solution of the second product, and freeze-drying to obtain a solid product; dissolving the solid product, adding Pd / C, introducing hydrogen gas, stirring and filtering to remove Pd / C, concentrating and freeze-drying to obtain the blood group trisaccharide. This method provides a new technical idea for the chemoenzymatic synthesis of blood group trisaccharide, which is conducive to popularization and application and solves the problems of low yield and high price.

The invention discloses a high-precision full-automatic blood type analyzer device which is characterized in that a bar code scanning head is arranged on the left side of a detection rack, a test tube rack is arranged on the right side of the bar code scanning head, a needle taking position is arranged on the right side of the test tube rack, a reagent position is arranged on the right side of the needle taking position, a needle removing position is arranged on the right side of the reagent position, and a diluent position is arranged on the front side of the needle removing position; a reagent card scanning head is arranged on the rear side of the sample adding position; a clamping position is arranged on the front side of the sample adding position; an oscillation incubation position is arranged on the right side of the sample adding position; a puncture position is arranged on the rear side of the oscillation incubation position; a rotating part of the centrifugal machine is provided with a centrifugal machine rotor, the left side of the detection rack guide rail is slidably connected with a sample adding arm, the right side of the detection rack guide rail is slidably connected with a clamping arm, and a rack body of the detection rack is slidably connected with a clamping bin.

Presented herein are systems and methods of producing “universal” and / or “off-the-shelf” CAR-T compositions suitable for cancer therapy to be administered to one or more individuals. A CAR-T composition is a composition comprising one or more types of chimeric antigen receptor T cells (CAR-T). The iPSCs and / or cell lines, and any iPSC-derived CAR-T compositions derived therefrom, are identified as compatible with one or more individuals using an identification of a cell type indicative of compatibility (e.g., HLA match and / or ABO blood match and / or RHD blood match). The compatible cells are then retrieved from a managed HLA-indexed (and / or otherwise indexed) repository or are derived from a biological sample of a donor. The retrieved compatible cells are then used to derive iPSC-derived CAR-T compositions, wherein the derived compositions are suitable for therapy of one or more individuals.

The utility model belongs to the technical field of red blood cell storage devices, and particularly relates to a red blood cell cold stock taking storage material box which comprises a storage material box body, a storage structure is arranged in the storage material box body and comprises a classification mechanism and an auxiliary mechanism, and the classification mechanism comprises a bearing plate, a guide rod, a classification plate, a first telescopic rod and a clamping plate. And one side of the bearing plate is connected with one end of a guide rod. According to the erythrocyte cold stock taking and storing box, stockers can be quickly and conveniently clamped and fixed, the stockers of erythrocytes of different blood types can be quickly and conveniently stored in a classified mode, and meanwhile the distance between a plurality of classification plates can be quickly and conveniently adjusted; according to the red blood cell storage box, different sizes of storage devices can be better clamped and fixed, the problem that red blood cells of different blood types cannot be quickly and conveniently classified and stored, and consequently re-distinguishing is needed when the red blood cells are taken is solved, and the working efficiency of the storage box is improved.

An adsorbent comprising at least two types of covalently bound antigens having the ability to bind at least one component present in a blood flow or blood plasma flow passing through said adsorbent is disclosed, as well as a column containing said adsorbent, a kit containing said column in a sealed plastic bag, a method for the production of said kit, a method for reduction or elimination of undesired blood group specific antibodies or autoimmune antibodies in connection with autoimmune diseases, a method for treatment of myasthenia gravis, and a method for treatment of rheumatoid arthritis.

The present application relates to the field of medical detection, and in particular, the present application relates to a kind of integrated genotyping method of multiple blood cell antigens related to selective blood transfusion and application thereof.The genotyping method described in the present application is an integrated typing method based on DNA sequence related to multiple red blood cell, platelet and white blood cell antigen coding genes related to blood transfusion, which can reduce the incidence of xenoantigen sensitization caused by alloimmunization, promote precision blood transfusion, and cover blood group genes including ABO, RhD, RhCE, HPA (ITGB3, GP1BA, GP1BB, ITGA2B, ITGA2, CD109, GP9), HLA-A, -B, -C, -DRB1, -DQB1, ABO, FUT1, FUT2, etc.The covered alleles can reach tens of thousands.Moreover, after the platform built in the present application, second-generation sequencing library construction and third-generation sequencing library construction can be carried out, which provides an excellent re-creation and reproduction platform for future haplotype confirmation of multiple site variations in blood group genes, and has important significance for future precision blood transfusion.

The invention relates to a breeding method of a fluoride-resistant and polyhedrosis-resistant bombyx mori strain, and belongs to the technical field of bombyx mori genetic breeding. In order to solve the technical problems that an existing silkworm variety cannot resist fluoride poisoning and pyosis at the same time, and resistance is prone to loss, the method comprises the following steps: hybridizing a germplasm carrying a fluorine-resistant major gene and a germplasm carrying an antiviral dominant major gene to obtain an F1 generation, and backcrossing the F1 generation with a fluorine-resistant parent after fluorine-resistant screening to obtain a BC1 generation; and finally, fluorine-resistant and antiviral characters are synchronously screened and improved through selfing generations, and directional breeding is carried out by combining whole-course recorded development and cocoon quality characters. According to the method, a new silkworm strain with double resistance is successfully bred, and the method is mainly used for solving the problem of low survival rate of silkworm breeding in a fluorine pollution area and a virus high incidence area in industrial production.

The invention discloses a method for accurately detecting fetal blood types based on a pregnant woman peripheral blood cff-DNA enrichment method, and belongs to the technical field of biomedical detection. According to the method, an acoustically responsive conformation lock aptamer probe is used, and the probe comprises a recognition domain for specifically recognizing placenta-derived nucleosome and a capture group shielded by an intramolecular conformation lock space. The conformational lock can be selectively opened by a resonant sound field of a specific frequency. In the implementation of the method, the probe in a locked state is mixed with a plasma sample, and then the mixture is processed in the multistage acoustic flow control micro-fluidic chip; and finally, capturing the compound in the activated state through magnetic beads, and eluting to obtain high-purity fetal nucleosome for subsequent DNA extraction and detection. According to the method, efficient, rapid and automatic enrichment of the target object is realized through innovative sequential control of purification and activation in sequence, and the purity and the yield are remarkably improved.

The invention discloses an affinity chromatography medium for removing an anti-A / anti-B antibody and a preparation method and application of the affinity chromatography medium, the general formula of the chromatography medium is shown in the formula I. M is a matrix, A comprises 2-15 carbon atoms and further comprises one or more hydroxyl groups and / or-NH-,-O-or-S-, S is a straight-chain or branched-chain alkyl group with 1-17 carbon atoms, and the formula I is shown in the description. D is a straight chain or branched chain alkyl group with 1-17 carbon atoms, n is 1 or 2, and T is a blood type saccharide ligand. The chromatography medium obtained in the invention has good stability and binding capacity.

The invention relates to a protease treatment reagent and application thereof in enzyme treatment of cells, and the protease treatment reagent is prepared by the following steps: adding L-cysteine, Pluronic, gelatin, a chelating agent and a bacteriostatic agent into a buffer agent, dissolving at room temperature, and obtaining the protease treatment reagent after complete dissolution; the protease treatment reagent is used as a grinding reagent to fully grind protease, the protease treatment reagent is used for fixing the volume, then a microporous filter is used for filtering to obtain a protease solution, and the obtained protease solution is used for treating red blood cells and further used for red blood cell blood group serological detection. The protease treatment reagent has a stable pH buffer environment, the protease activity can be effectively protected, the protease is pretreated by the protease treatment reagent to form a protease solution, and the protease solution can be stably stored for a long time; an original enzyme treatment cell method is converted into a unified, controllable and standardized enzyme treatment cell production mode from a laboratory method, and the utilization rate of protease and the success rate of enzyme treatment cells are greatly improved.

The application discloses a kind of based on centrifugal microfluidic chip's full-automatic blood type analyzer, belong to blood type analysis equipment technical field.The analyzer includes bottom plate, and sample reagent bin module is arranged on bottom plate, chip processing module, new chip storage bin module, chip recycling bin module, sample adding arm module and chip pusher module;Chip pusher module is used to contact and promote microfluidic chip from new chip storage bin module translation to chip processing module processing, and promote after microfluidic chip translation to chip recycling bin module recycling after chip processing module processing;Sample adding arm module is located at the top of sample reagent bin module and chip processing module, for adding sample or reagent.The application is based on centrifugal microfluidic chip, realizes full-automatic sample and reagent addition, chip transfer, chip loading, chip centrifugation, result detection judgment and chip recycling, without manual intervention, support sample no shutdown sample, sample into result, smaller volume under same test speed.

The present application relates to a kind of primer set and kit for Sanger sequencing method for human RHD blood group genotyping detection, belong to biomedical clinical molecular detection field.The primer set for Sanger sequencing method for human RHD blood group genotyping detection of the present application, including specific PCR amplification primer set, the specific PCR amplification primer set includes 9 pairs of amplification primer designed according to RHD gene specific sequence;The primer set also includes 10 RHD specific sequencing primers corresponding to amplification product.The present application has the following technical effects: the present application adopts the RHD gene specific amplification primer designed by ARMS and homologous sequence specific base method, and specificity and accuracy are higher;With the RHD specific sequencing primer of the present application, the gene sequence of the sample to be detected is detected by Sanger sequencing method, RHD genotype is interpreted, and new mutation point can be found.The identification result of RHD blood group gene 1 to 10 exons can be obtained by the kit of the present application, so as to accurately determine the RHD gene typing of experimental sample.

The blood transfusion bag storage device comprises a refrigeration box body, a first refrigeration cavity is formed in the front end of the refrigeration box body, a first box door is arranged at the opening end of the first refrigeration cavity, a plurality of partition plates are arranged in the first refrigeration cavity and divide the first refrigeration cavity into at least six installation grooves, and storage assemblies are arranged in the installation grooves. The storage assembly is slidably connected to the mounting groove, the storage assembly is used for storing a blood transfusion bag, a power assembly is arranged in the refrigeration box and pushes the storage assembly to slide front and back, and the storage assembly comprises a mounting base, a weighing sensor and a placement groove. The storage assembly is pushed out of the installation groove through the power assembly, the containing cavities are sequentially exposed, a nurse can conveniently take out blood transfusion bags of corresponding blood types, and the problem of wrong taking is avoided.

Disclosed herein are compositions and methods relating to genetically engineered mammalian cells that include modulating the expression of selected genes. The genetically engineered mammalian cells described herein advantageously have improved viability. In some embodiments, the mammalian cells are genetically engineered to reduce or eliminate expression of a renalase gene compared to the expression level of the same cell type that is not genetically engineered, and the cells are further genetically engineered to reduce or eliminate expression of an ABO gene.

The present invention describes a whole blood assay chip that enables separation of the cellular compartment from surrounding plasma, facilitating multiplexed measurement of otherwise difficult to detect soluble factors produced upon cellular stimulation. The invention is a microfluidic chip with an incubation chamber comprising an inlet, an outlet, and a fluidic barrier. Using this platform, the cellular compartment can be stimulated with various substances and levels of immunologic biomarkers could be measured. Through a proprietary immunologic algorithm described herein, this would allow for the diagnosis of an allergic response to foods, antibiotics, and / or other drugs. Additional applications of the invention besides allergy testing could include pregnancy testing, blood type, and medical applications requiring whole blood cellular stimulation readouts.

This invention discloses a composite material based on hollow mesoporous carbon spheres loaded with ABO blood group monoclonal antibodies, comprising hollow mesoporous carbon spheres and ABO blood group monoclonal antibodies loaded on the hollow mesoporous carbon spheres, wherein the ABO blood group monoclonal antibodies are selected from at least one of type A antibodies and type B antibodies. This invention also discloses a method for preparing the composite material, comprising: modifying the surface of the hollow mesoporous carbon spheres with amino groups; dispersing the spheres in an ABO blood group monoclonal antibody solution; and stirring the reaction. This invention immobilizes ABO blood group monoclonal antibodies on the surface of hollow mesoporous carbon spheres, improving the stability, specificity, and sensitivity of the antibodies, and achieving rapid and accurate ABO blood group identification. This invention also discloses a kit comprising the above-mentioned composite material, and the application of this composite material in ABO blood group detection.

This invention discloses the application of 6-benzylaminopurine and drugs containing 6-benzylaminopurine in the prevention and / or treatment of hemorrhagic septicemia in silkworms. Using 6-benzylaminopurine as the core active ingredient, drugs for the prevention and treatment of hemorrhagic septicemia in silkworms can be prepared. Experiments show that when the final concentration of 6-benzylaminopurine reaches 50 μM, it can significantly inhibit the replication of BmNPV, and can still exert an inhibitory effect after the virus has completed cell infection, demonstrating good therapeutic efficacy. This invention provides a new solution for the research and development and production of drugs for hemorrhagic septicemia in silkworms, and has broad application prospects and promotional value.

The present invention discloses an anti-idiotypic antibody that neutralizes anti-D antibodies, which comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region contains HCDR1, HCDR2, and HCDR3 sequences, and the light chain variable region contains LCDR1, LCDR2, and LCDR3 sequences, wherein: the amino acid sequence of HCDR1 is as shown in SEQ ID No.1; the amino acid sequence of HCDR2 is as shown in SEQ ID No.2; the amino acid sequence of HCDR3 is as shown in SEQ ID No.3. The amino acid sequence of LCDR1 is as shown in SEQ ID No.4; the amino acid sequence of LCDR2 is as shown in SEQ ID No.5; the amino acid sequence of LCDR3 is as shown in SEQ ID No.6. This anti-idiotypic antibody can specifically bind to anti-D antibodies and can be applied to clinical blood group detection or the prevention or treatment of anti-D antibody-related diseases.

This invention relates to the fields of molecular diagnostics and gene sequencing technology, specifically to a primer set, amplification system, amplification method, library construction method, and sequencing method for Kidd blood group system genotyping amplification based on long-read nanopore sequencing. This invention provides primers and an optimized amplification system for specifically amplifying long fragments of the Kidd blood group encoding gene SLC14A1; a library construction method adapted for nanopore sequencing; and a SLC14A1 genotyping method based on long-read data for haplotype analysis. This invention overcomes the limitations of conventional techniques that only detect single SNPs, enabling the simultaneous acquisition of the complete sequence and phase information of key regions of the SLC14A1 gene, achieving accurate genotyping of JKA / JKB and effective identification of rare variants such as weakly expressed and deleted variants. This invention provides a powerful molecular tool for clinical transfusion safety assurance, rare blood group bank construction, and genetic research.

The present invention pertains to humanized anti-Lewis Y antibodies which specifically bind to Lewis Y and do not show any cross-reactivity. Especially, the humanized anti-Lewis Y antibodies do not bind to Lewis b or any other blood group carbohydrate antigen. In particular, the present invention is directed to humanized anti-Lewis Y antibodies which are useful in the treatment of cancer.