49 results about "Blood type" patented technology

The invention discloses a pharmaceutical composition for preventing and treating chronic atrophic gastritis. The pharmaceutical composition is prepared from the following raw materials in the following weight ratio: 14-18 parts of peach kernel, 10-14 parts of Chuanxiong rhizome, 8-12 parts of Panax notoginseng, 6-10 parts of safflower, 20-28 parts of kelp, 10-14 parts of selfheal, 15-25 parts of codonopsis pilosula, 10-14 parts of atractylodes macrocephala, 12-16 parts of fructus aurantii, 8-12 parts of amomum villosum, 12-16 parts of bupleurum, 10-18 parts of citron, and 5-10 parts of fucoidan. The pharmaceutical composition is used to treat chronic atrophic gastritis of gastric collateral blood stasis type by promoting blood circulation and removing blood stasis, unblocking meridians and relieving pain, eliminating phlegm, softening and dispersing nodules, invigorating qi and promoting qi circulation, and soothing the liver and harmonizing the stomach. The invention also discloses a preparation method of the pharmaceutical composition.

The utility model discloses a blood transport box convenient to take, which belongs to the technical field of blood transport, and comprises a box body and a handle, the box body is connected with the handle, the box body comprises a storage space, a first partition plate and a second partition plate are arranged in the box body, and the first partition plate and the second partition plate are respectively arranged in the storage space. The first partition plate and the second partition plate divide the storage space into a first storage space, a second storage space and a third storage space, and a first storage mechanism, a second storage mechanism and a third storage mechanism are arranged in the first storage space, the second storage space and the third storage space correspondingly. The blood bag storage box has the advantages that blood bags can be placed in order through the first storage mechanism, the second storage mechanism and the third storage mechanism, stacking of the blood bags is avoided, when a user needs to take the blood bags of specific blood types, the user does not need to rummage the whole box body, only the first storage mechanism, the second storage mechanism or the third storage mechanism needs to be opened, and the blood bags can be conveniently stored. And the required blood bag is directly taken out, so that the taking convenience is greatly improved.

The invention relates to a primer group for detecting P1PK and Lewis blood group genotyping, a kit and application, and relates to the technical field of biology. The primer group comprises 12 groups of primer groups, and the nucleotide sequences of the 12 groups of primer groups are as shown in SEQ ID NO: 1-36. The 12 primer groups respectively correspond to 12 detection sites of P1PK and Lewis blood group systems, and the established detection method can effectively detect mutation conditions of 12 SNP sites, so that genetic typing of two blood group systems is realized. Compared with the existing detection technology, the method has the advantages that multi-system and multi-site simultaneous detection can be realized, the detection efficiency and the reaction sensitivity are improved, the defects of complicated operation, tedious process and the like of the existing detection method are overcome, and the method has a remarkable clinical application value.

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

The invention discloses a control method of blood processing chips. The blood processing chips are loaded in a centrifugal machine; upper plasma and red blood cells are separated based on first centrifugation of blood, and normal saline enters a mixing area; the centrifugal machine stops working, the red blood cells sequentially enter a siphon flow channel and sequentially trigger second-time centrifugation and third-time centrifugation, the siphon flow channel is in an open state, and the red blood cells enter the mixing area from the red blood cell quantifying area; the red blood cells are layered with normal saline in the mixing area due to centrifugal action; carrying out fourth centrifugation, carrying out positive and negative rotation of the centrifugal machine, carrying out re-uniform mixing through oscillation, carrying out red blood cell resuspension, and carrying out multiple times of separation on the blood along the vertical direction; rotating the centrifugal tray by 90 degrees, standing for 3 minutes, and enabling the resuspension in the uniform mixing area to enter a capillary quantitative flow channel; the blood processing chip is matched with the detection card and judges the blood type, and the blood sample, the normal saline and the reverse red blood cells are located in the blood processing chip and are separated for multiple times in the blood processing chip.

The present invention relates to providing protective immunity against Leptospira serovar Icterohaemorrhagiae to dogs using a vaccine comprising a non-Icterohaemorrhagiae Leptospira serotype.

Blood Group and Glucose Detection Device

The present disclosure provides methods and kits for detecting Group A Streptococcus in biological samples. More particularly, the present disclosure provides methods for enhancing the specificity and sensitivity of Group A Streptococcus immunoassays by including N-propionyl-D-glucosamine, 2-N-butanoyl-D-glucosamide, Bis-(2-(D-2-deoxy-glucosaminyl))-PEG3-amide, m-PEG4-glucosamine, m-PEG6-glucosamine, or m-PEG10-glucosamine. The methods and kits disclosed herein are thus useful for reliable and early diagnosis of streptococcal infections in a subject.

The present invention relates to humanized anti-Lewis Y antibodies that specifically bind Lewis Y and do not show any cross-reactivity. In particular, the humanized anti-Lewis Y antibodies do not bind to Lewis b or any other blood group carbohydrate antigen. In particular, the present invention relates to humanized anti-Lewis Y antibodies for use in the treatment of cancer.

A method and a kit for genotyping of multi-system red blood cell (RBC) blood group based on next-generation sequencing (NGS) are provided. Multiplex PCR capture primer sets and amplification reaction conditions thereof can be designed using Illumina as a platform to simultaneously detect full-length coding regions and spliced flanking regions of 25 blood group genes in 17 RBC blood group systems among three reactions, thereby enabling rapid genotyping of the 17 RBC blood group systems and multiple key rare blood groups therein. The method and the kit are based on high-throughput sequencing and have a high detection efficiency and a low average cost; the method and the kit also break through a limitation of existing blood group gene detection technology that cannot distinguish haplotypes; moreover, variations including known mutation sites and unknown mutation sites can be detected to obtain comprehensive and accurate detection results.

The invention provides a puncture device and a blood type analyser, the puncture device comprises a grabbing mechanism, an elastic mechanism and a puncture piece, the elastic mechanism comprises an elastic piece with a first pop-up part and a second pop-up part, the puncture piece is internally provided with a moving channel, the first pop-up part of the elastic piece can extend to the position below the moving channel, and the second pop-up part of the elastic piece can extend to the position below the moving channel; a second pop-up part of the elastic part can abut against an abutting part in the moving channel to assist separation of the blood card and the puncture needle, so that the probability that the puncture part is stuck in the separation process of the puncture part and the grabbing mechanism is reduced, taking, placing and replacing of the puncture part are smoother, and the work efficiency is improved. The elastic piece does not need to be installed on the puncture piece, the installation space of the elastic piece does not need to be reserved in the puncture piece, and the size of the puncture block can be reduced.

This invention relates to the fields of molecular diagnostics and gene sequencing technology, specifically to a complete solution for high-precision genotyping of the human Lewis blood group system. The invention provides primer pairs for specifically amplifying long fragments of the Lewis blood group coding genes FUT2 and FUT3, and an optimized single amplification system; a library construction method adapted for nanopore sequencing; and a Lewis (FUT2, FUT3) genotyping method based on long-read data for haplotype analysis. This invention overcomes the detection limitations of conventional techniques, simultaneously acquiring the complete sequence and phase information of key regions of the FUT2 and FUT3 genes through a single amplification system, achieving accurate genotyping of common Lewis blood group phenotypes (Le(a+b−), Le(a−b+), Le(a−b−)) and effective identification of rare variants such as weakly expressed and deleted variants.

The present invention provides a chemoenzymatic synthesis method for blood group trisaccharide, which includes: dissolving a substrate, GDP-Fuc, and magnesium chloride in water, adjusting the pH of the solution, adding the enzyme α1,2-FucT, and stirring for reaction to obtain a first product reaction solution; adding a de-enzyme solvent, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the first product; adding a glycosylated product, adjusting the pH after dissolution, and adding a glycosyltransferase to obtain a second product reaction solution; using a de-enzyme solvent to remove the glycosyltransferase, standing, centrifuging and filtering, taking the supernatant, and evaporating to remove the de-enzyme solvent to obtain an aqueous solution of the second product; separating and purifying the aqueous solution of the second product, and freeze-drying to obtain a solid product; dissolving the solid product, adding Pd / C, introducing hydrogen gas, stirring and filtering to remove Pd / C, concentrating and freeze-drying to obtain the blood group trisaccharide. This method provides a new technical idea for the chemoenzymatic synthesis of blood group trisaccharide, which is conducive to popularization and application and solves the problems of low yield and high price.

The invention discloses a high-precision full-automatic blood type analyzer device which is characterized in that a bar code scanning head is arranged on the left side of a detection rack, a test tube rack is arranged on the right side of the bar code scanning head, a needle taking position is arranged on the right side of the test tube rack, a reagent position is arranged on the right side of the needle taking position, a needle removing position is arranged on the right side of the reagent position, and a diluent position is arranged on the front side of the needle removing position; a reagent card scanning head is arranged on the rear side of the sample adding position; a clamping position is arranged on the front side of the sample adding position; an oscillation incubation position is arranged on the right side of the sample adding position; a puncture position is arranged on the rear side of the oscillation incubation position; a rotating part of the centrifugal machine is provided with a centrifugal machine rotor, the left side of the detection rack guide rail is slidably connected with a sample adding arm, the right side of the detection rack guide rail is slidably connected with a clamping arm, and a rack body of the detection rack is slidably connected with a clamping bin.

The invention provides a chemical enzymatic synthesis method of blood group trisaccharide, which comprises the following steps: dissolving a substrate, GDP-Fuc and magnesium chloride in water, adjusting the pH value of the solution, adding enzyme alpha1 and 2-FucT, and stirring for reaction to obtain a first product reaction solution; adding an enzyme-removing solvent, standing, centrifugally filtering, taking supernatant, and evaporating to remove the enzyme-removing solvent, so as to obtain a first product aqueous solution; adding glycosylate, dissolving, adjusting the pH value, and adding glycosyltransferase to obtain a second product reaction solution; removing the glycosyl transferase by using an enzyme removal solvent, standing, centrifugally filtering, taking supernatant, and evaporating to remove the enzyme removal solvent, so as to obtain a second product aqueous solution; separating and purifying the second product aqueous solution, and freeze-drying to obtain a solid product; dissolving the solid product, adding Pd / C, introducing hydrogen, stirring, filtering to remove Pd / C, concentrating and freeze-drying to obtain the hemotriose. The method provides a new technical thought of synthesizing the blood group trisaccharide by a chemical enzyme method, is beneficial to popularization and application, and solves the problems of low yield and high price.

Presented herein are systems and methods of producing “universal” and / or “off-the-shelf” CAR-T compositions suitable for cancer therapy to be administered to one or more individuals. A CAR-T composition is a composition comprising one or more types of chimeric antigen receptor T cells (CAR-T). The iPSCs and / or cell lines, and any iPSC-derived CAR-T compositions derived therefrom, are identified as compatible with one or more individuals using an identification of a cell type indicative of compatibility (e.g., HLA match and / or ABO blood match and / or RHD blood match). The compatible cells are then retrieved from a managed HLA-indexed (and / or otherwise indexed) repository or are derived from a biological sample of a donor. The retrieved compatible cells are then used to derive iPSC-derived CAR-T compositions, wherein the derived compositions are suitable for therapy of one or more individuals.

The utility model relates to the technical field of medical instruments, and discloses a micro-column gel card puncture structure and a blood type analyzer, the micro-column gel card puncture structure comprises a bearing disc, a first puncture assembly and a second puncture assembly; the bearing disc is used for bearing reagent cards; the first puncture assembly comprises a first number of first puncture needles; the second puncture assembly comprises a second number of second puncture needles, and the first puncture needles and the second puncture needles are used for puncturing the surface of the reagent card; wherein the first number is greater than the second number; when the number of the surfaces of the reagent cards needing to be punctured is a first number or integral multiples of the first number, puncturing by using the first number of first puncture needles; when the number of the surfaces of the reagent cards needing to be punctured is the second number, the second number of second puncture needles are used for puncturing. The micro-column gel card solves the problem of waste caused by a puncture mode adopted by an existing micro-column gel card.

The utility model belongs to the technical field of red blood cell storage devices, and particularly relates to a red blood cell cold stock taking storage material box which comprises a storage material box body, a storage structure is arranged in the storage material box body and comprises a classification mechanism and an auxiliary mechanism, and the classification mechanism comprises a bearing plate, a guide rod, a classification plate, a first telescopic rod and a clamping plate. And one side of the bearing plate is connected with one end of a guide rod. According to the erythrocyte cold stock taking and storing box, stockers can be quickly and conveniently clamped and fixed, the stockers of erythrocytes of different blood types can be quickly and conveniently stored in a classified mode, and meanwhile the distance between a plurality of classification plates can be quickly and conveniently adjusted; according to the red blood cell storage box, different sizes of storage devices can be better clamped and fixed, the problem that red blood cells of different blood types cannot be quickly and conveniently classified and stored, and consequently re-distinguishing is needed when the red blood cells are taken is solved, and the working efficiency of the storage box is improved.

An adsorbent comprising at least two types of covalently bound antigens having the ability to bind at least one component present in a blood flow or blood plasma flow passing through said adsorbent is disclosed, as well as a column containing said adsorbent, a kit containing said column in a sealed plastic bag, a method for the production of said kit, a method for reduction or elimination of undesired blood group specific antibodies or autoimmune antibodies in connection with autoimmune diseases, a method for treatment of myasthenia gravis, and a method for treatment of rheumatoid arthritis.

The present application relates to the field of medical detection, and in particular, the present application relates to a kind of integrated genotyping method of multiple blood cell antigens related to selective blood transfusion and application thereof.The genotyping method described in the present application is an integrated typing method based on DNA sequence related to multiple red blood cell, platelet and white blood cell antigen coding genes related to blood transfusion, which can reduce the incidence of xenoantigen sensitization caused by alloimmunization, promote precision blood transfusion, and cover blood group genes including ABO, RhD, RhCE, HPA (ITGB3, GP1BA, GP1BB, ITGA2B, ITGA2, CD109, GP9), HLA-A, -B, -C, -DRB1, -DQB1, ABO, FUT1, FUT2, etc.The covered alleles can reach tens of thousands.Moreover, after the platform built in the present application, second-generation sequencing library construction and third-generation sequencing library construction can be carried out, which provides an excellent re-creation and reproduction platform for future haplotype confirmation of multiple site variations in blood group genes, and has important significance for future precision blood transfusion.

The invention relates to a breeding method of a fluoride-resistant and polyhedrosis-resistant bombyx mori strain, and belongs to the technical field of bombyx mori genetic breeding. In order to solve the technical problems that an existing silkworm variety cannot resist fluoride poisoning and pyosis at the same time, and resistance is prone to loss, the method comprises the following steps: hybridizing a germplasm carrying a fluorine-resistant major gene and a germplasm carrying an antiviral dominant major gene to obtain an F1 generation, and backcrossing the F1 generation with a fluorine-resistant parent after fluorine-resistant screening to obtain a BC1 generation; and finally, fluorine-resistant and antiviral characters are synchronously screened and improved through selfing generations, and directional breeding is carried out by combining whole-course recorded development and cocoon quality characters. According to the method, a new silkworm strain with double resistance is successfully bred, and the method is mainly used for solving the problem of low survival rate of silkworm breeding in a fluorine pollution area and a virus high incidence area in industrial production.

The present invention provides cells with enhanced immune checkpoint engagement function (immune checkpoint engager "ICE" cells). In some embodiments, the ICE cells have PD-1 engagement function (PD-1 engager cells). The ICE cells attenuate adaptive and innate immunity when transplanted into a subject, compared to parental cells with corresponding unmodified immune checkpoint engagement function. In some embodiments, the PD-1 engager cells are hypoimmune pluripotent (HIP) cells. In further embodiments, the HIP cells are blood type O (HIPO), Rh factor (Rh) negative (HIP-), or blood type O and Rh- (HIPO-). In other embodiments, the PD-1 engager cells are derived or differentiated from HIP cells, HIP- cells, or HIPO- cells. In other embodiments, the ICE cells express a ligand that binds to an inhibitory immune cell receptor selected from the group consisting of LILRB1 engager, LILRB3 engager, and TIM3 engager. In other embodiments, the ICE cells are PD-1 engager cells that further express a ligand that binds to another inhibitory immune cell receptor selected from the group consisting of CD47, truncated CD47, LILRB1 engager, LILRB3 engager, and TIM3 engager.

The invention discloses a method for accurately detecting fetal blood types based on a pregnant woman peripheral blood cff-DNA enrichment method, and belongs to the technical field of biomedical detection. According to the method, an acoustically responsive conformation lock aptamer probe is used, and the probe comprises a recognition domain for specifically recognizing placenta-derived nucleosome and a capture group shielded by an intramolecular conformation lock space. The conformational lock can be selectively opened by a resonant sound field of a specific frequency. In the implementation of the method, the probe in a locked state is mixed with a plasma sample, and then the mixture is processed in the multistage acoustic flow control micro-fluidic chip; and finally, capturing the compound in the activated state through magnetic beads, and eluting to obtain high-purity fetal nucleosome for subsequent DNA extraction and detection. According to the method, efficient, rapid and automatic enrichment of the target object is realized through innovative sequential control of purification and activation in sequence, and the purity and the yield are remarkably improved.

The invention discloses an affinity chromatography medium for removing an anti-A / anti-B antibody and a preparation method and application of the affinity chromatography medium, the general formula of the chromatography medium is shown in the formula I. M is a matrix, A comprises 2-15 carbon atoms and further comprises one or more hydroxyl groups and / or-NH-,-O-or-S-, S is a straight-chain or branched-chain alkyl group with 1-17 carbon atoms, and the formula I is shown in the description. D is a straight chain or branched chain alkyl group with 1-17 carbon atoms, n is 1 or 2, and T is a blood type saccharide ligand. The chromatography medium obtained in the invention has good stability and binding capacity.

The invention relates to a protease treatment reagent and application thereof in enzyme treatment of cells, and the protease treatment reagent is prepared by the following steps: adding L-cysteine, Pluronic, gelatin, a chelating agent and a bacteriostatic agent into a buffer agent, dissolving at room temperature, and obtaining the protease treatment reagent after complete dissolution; the protease treatment reagent is used as a grinding reagent to fully grind protease, the protease treatment reagent is used for fixing the volume, then a microporous filter is used for filtering to obtain a protease solution, and the obtained protease solution is used for treating red blood cells and further used for red blood cell blood group serological detection. The protease treatment reagent has a stable pH buffer environment, the protease activity can be effectively protected, the protease is pretreated by the protease treatment reagent to form a protease solution, and the protease solution can be stably stored for a long time; an original enzyme treatment cell method is converted into a unified, controllable and standardized enzyme treatment cell production mode from a laboratory method, and the utilization rate of protease and the success rate of enzyme treatment cells are greatly improved.

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and a B subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP sites of the ABO blood group variant comprise the 648th site c. 648Cgt from an initiation codon of an ABO gene coding region; g mutation. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of a B subtype variant blood group identification method and product, so that the substance can be effectively applied to rapid detection of patients and blood donors, the B subtype variant blood group of an individual can be defined, and potential possible blood transfusion reaction can be prevented in time.

The application discloses a kind of based on centrifugal microfluidic chip's full-automatic blood type analyzer, belong to blood type analysis equipment technical field.The analyzer includes bottom plate, and sample reagent bin module is arranged on bottom plate, chip processing module, new chip storage bin module, chip recycling bin module, sample adding arm module and chip pusher module;Chip pusher module is used to contact and promote microfluidic chip from new chip storage bin module translation to chip processing module processing, and promote after microfluidic chip translation to chip recycling bin module recycling after chip processing module processing;Sample adding arm module is located at the top of sample reagent bin module and chip processing module, for adding sample or reagent.The application is based on centrifugal microfluidic chip, realizes full-automatic sample and reagent addition, chip transfer, chip loading, chip centrifugation, result detection judgment and chip recycling, without manual intervention, support sample no shutdown sample, sample into result, smaller volume under same test speed.

The present application relates to a kind of primer set and kit for Sanger sequencing method for human RHD blood group genotyping detection, belong to biomedical clinical molecular detection field.The primer set for Sanger sequencing method for human RHD blood group genotyping detection of the present application, including specific PCR amplification primer set, the specific PCR amplification primer set includes 9 pairs of amplification primer designed according to RHD gene specific sequence;The primer set also includes 10 RHD specific sequencing primers corresponding to amplification product.The present application has the following technical effects: the present application adopts the RHD gene specific amplification primer designed by ARMS and homologous sequence specific base method, and specificity and accuracy are higher;With the RHD specific sequencing primer of the present application, the gene sequence of the sample to be detected is detected by Sanger sequencing method, RHD genotype is interpreted, and new mutation point can be found.The identification result of RHD blood group gene 1 to 10 exons can be obtained by the kit of the present application, so as to accurately determine the RHD gene typing of experimental sample.

The blood transfusion bag storage device comprises a refrigeration box body, a first refrigeration cavity is formed in the front end of the refrigeration box body, a first box door is arranged at the opening end of the first refrigeration cavity, a plurality of partition plates are arranged in the first refrigeration cavity and divide the first refrigeration cavity into at least six installation grooves, and storage assemblies are arranged in the installation grooves. The storage assembly is slidably connected to the mounting groove, the storage assembly is used for storing a blood transfusion bag, a power assembly is arranged in the refrigeration box and pushes the storage assembly to slide front and back, and the storage assembly comprises a mounting base, a weighing sensor and a placement groove. The storage assembly is pushed out of the installation groove through the power assembly, the containing cavities are sequentially exposed, a nurse can conveniently take out blood transfusion bags of corresponding blood types, and the problem of wrong taking is avoided.

Disclosed herein are compositions and methods relating to genetically engineered mammalian cells that include modulating the expression of selected genes. The genetically engineered mammalian cells described herein advantageously have improved viability. In some embodiments, the mammalian cells are genetically engineered to reduce or eliminate expression of a renalase gene compared to the expression level of the same cell type that is not genetically engineered, and the cells are further genetically engineered to reduce or eliminate expression of an ABO gene.