20 results about "Cellular compartment" patented technology

The present invention relates to a multi-compartment microfluidic device comprising a first cellular compartment (10) within which a target organ is reproduced, a second cellular compartment (20), located below the first cellular compartment (10) and separated from it by a porous membrane (30), and a third cellular compartment (40) connected to the first cellular compartment (10) by a means forming a biological interface (50). Figure 1

The present invention relates to a multi-compartment microfluidic device comprising a first cellular compartment (10) in which a target organ of the digestive system is reproduced and a second cellular compartment (40) comprising neurons (400), the second cellular compartment (40) being connected to the first cellular compartment (10) by a means forming a biological interface (50) to allow communication via neuronal connection (401) between the first cellular compartment (10) and the second cellular compartment (40). Figure 1

The present application relates to a kind of cytoplasm-specific expression PPM1K gene delivery system and its preparation method and application, belong to gene delivery system preparation technical field.The gene delivery system is preferably adeno-associated virus (AAV), and its preparation method specifically includes the following steps: PPM1K gene or its functional variant is inserted into vector, detectable label is added at C-terminal, and recombination plasmid is formed;Recombinant plasmid is transformed into competent e. coli and is amplified, after plasmid is screened, recombination AAV plasmid is obtained;Recombinant AAV plasmid, auxiliary plasmid and capsid plasmid are co-transfected into packaging cell using transfection reagent, after transfection, cell and culture supernatant are collected, freeze-thaw buffer is added repeatedly freeze-thaw, and cell lysate is obtained;After centrifugation of cell lysate, supernatant is taken, and purified filtration is carried out.The present application realizes the cell compartment specificity expression of treatment gene by vector design, and shows significant treatment effect and safety advantage.

Aspects of the present application provide methods for analyzing biological samples. The methods can include flowing a biological sample through a fluidic channel, wherein individual cells from the biological sample can be compartmentalized. In certain aspects, at least 105 cells from the biological sample can be compartmentalized. At least a subset of the compartmentalized cells can be lysed to release nucleic acids, and the nucleic acids can be captured and / or analyzed.

Methods of treating heart disease by modulating the cellular localization of the beta isoform calcium / calmodulin dependent protein kinase II delta (CaMKIIδ) via the disruption of post-transcriptional phosphorylation.

A culture system for human dermal and epidermal layers of the skin is provided, which culture reproduces the histomorphology of native human skin. The system is highly reproducible and maintains the cellular diversity of human skin for extended periods of time, both in the types of cells that are present, and in the architecture of the tissue. Features of the culture include self-production of molecules and proteins required for maintenance and maturation of the diverse cellular compartments.

Hie disclosure provides methods for cell-based screening of genetically encoded protein binders, involving (a) providing recombinant eukaryotic cells that have a first nucleic acid encoding a membrane protein, and / or a secreted protein; and a second nucleic acid encoding a first fusion protein comprising one or more recombinant protein target-binding candidate fused to (A) a direct cellular compartment retention tag, or (B) an indirect cellular compartment retention tag; wherein the protein target-binding candidate, when expressed, is retained within a cellular compartment by means of the cellular compartment retention tag; (b) culturing the recombinant eukaryotic cell under conditions suitable to express the recombinant protein target and the protein target-binding candidate in the recombinant eukaryotic cell; and (c)detecting localization of the protein target in the recombinant eukaryotic cell in one or more of the cellular compartments.

The invention belongs to the technical field of subcellular level detection and analysis, and discloses a nanocapillary, a dual-circuit sensing element based on the nanocapillary and application of the dual-circuit sensing element. The nano capillary tube is of a multi-layer composite structure, and according to the double-circuit sensing element constructed based on the nano capillary tube, two independent circuits are integrated on the inner surface and the outer surface of the nano capillary tube respectively, so that accurate isolation and non-interference analysis of adjacent micro / nano-scale subcellular compartments are achieved; the design of the nanocapillary and the dual-circuit sensing element constructed based on the nanocapillary can be used as a multifunctional tool for researching the interaction between an organelle and surrounding cytoplasm.

The invention provides recombinant yarrowia lipolytica for producing beta-elemene in a subcellular compartment and a construction method and application of the recombinant yarrowia lipolytica, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica is prepared by taking yarrowia lipolytica as an original strain, performing localization expression on a germacene A synthase coding gene in a liposome through a liposome localization signal Oleosin, and knocking out a peroxisome biogenic factor 10 in a yarrowia lipolytica genome, so as to obtain the recombinant yarrowia lipolytica. And inserting into a 3-hydroxy-3-methylglutaryl CoA reductase expression cassette, a farnesyl pyrophosphate synthase expression cassette, an isopentenyl pyrophosphate isomerase expression cassette, a diacylglycerol acyltransferase expression cassette and a sterol acyltransferase expression cassette to obtain the recombinant expression vector. The recombinant yarrowia lipolytica disclosed by the invention can be used for efficiently producing beta-elemene.

Provided herein are methods for: (1) rapidly depleting circulating B cells and remodeling one or more B cell compartments, (2) treating B cell-related autoimmune diseases, and (3) B cell malignancies, using B cell depleting antibodies that are directed to CD19 or CD20 and one or more target antigens. Further provided herein is a CD19 / CD20 bispecific antibody with dual Fc domains that mediate enhanced effector functions and B cell depletion. HB2198 demonstrated enhanced binding to Fcγ receptors, potent effector functions, and efficient depletion of B cells in vitro and in vivo.

The present application relates to a kind of molecular chaperone, nano-carrier and its preparation method and application.The molecular chaperone is the recombinant histidine-sulphur oxygen protein coupled with single-stranded DNA, and the cysteine residue is introduced in the protein and coupled with the single-stranded DNA of amino modification.The nano-carrier is DNA tetrahedron, and the DNA tetrahedron is obtained by self-assembly based on base pairing principle from multiple DNA single strands.DNA nano-carrier is used to deliver the molecular chaperone, can realize HTrX to specific subcellular compartment, i.e.the targeted delivery of ribosome, so that the protein in ribosome exerts biological effect, reduces the negative influence of oxidative stress on protein synthesis to reverse the aging process.The present application also discloses the application of the above-mentioned molecular chaperone and nano-carrier in preparing anti-aging drugs.

The present disclosure relates to compositions and methods for tracking and spatially localizing a cell-expressed fusion protein within the cell (with the fusion protein optionally associated with a subcellular compartment, organelle, synapse, or the like), in a manner that minimizes any disruptive impact upon the cell, at least until the detection process is initiated. Use of transcriptomics and / or barcode nucleic acid detection is employed to assess both spatial localization of intracellularly tagged fusion proteins and to establish cell-cell connectivity, e.g., in neurons across a synapse, by associating axonal identities with individual neurons at the molecular tag and transcriptome level.

Aspects of a microneedle array containing a metal-organic structure (MOF)-vaccine biocomposite and a method for using the same are disclosed herein. The microneedle array further comprises a soluble material that dissolves when inserted into the skin of a subject, thereby releasing the MOF-vaccine biocomposite. The MOF may dissolve in an acidic environment, thereby being selected to target vaccine delivery to specific cellular compartments. Methods for preparing the MOF-vaccine biocomposite and the microneedle array are also disclosed.

The invention relates to a proximity marker conjugate of lipid nanomaterial interacting protein and application of the proximity marker conjugate, and belongs to the technical field of biology. The proximity marker conjugate of the interaction protein of the lipid nanomaterial is obtained by coupling the lipid nanomaterial and biotin ligase; the lipid nanomaterial is used for marking protein interacting with the lipid nanomaterial in vivo. Functional biotin ligase is purified and then coupled to the surface of the lipid nanomaterial, in-vivo protein crown proximity labeling is achieved, and then efficient, accurate and dynamic analysis of the lipid nanomaterial in the whole in-vivo biological interaction process is achieved. The method can capture instantaneous or weak interaction, and is high in sensitivity. The method is suitable for low-abundance proteins and membrane proteins, can reserve physiological status information when being used for marking in living cells, and is not limited by cell compartment localization.

The present invention provides novel artificial nucleic acid molecules encoding at least one antigenic peptide or protein and at least one additional sequence preferably targeting the antigenic peptides or proteins to cellular compartments of interest. Further, the invention provides (pharmaceutical) compositions or vaccines and kits comprising said nucleic acid molecules. The nucleic acid molecules, (pharmaceutical) compositions or vaccines and kits are useful for treating a variety of diseases such as cancer, infectious diseases, autoimmune diseases, allergies or graft-versus host disease.

The invention relates to a system and methods for enhancing access to nuclear informational molecules, such as DNA, RNA, and proteins, by analytical biomolecules, such as transposome complexes, by treating nuclei with a nuclear permeability enhancer, and to methods of using nuclear membrane, cell membrane, and external compartmentalization approaches as contiguity preserving elements.

The present invention discloses superoxide-specific quinone methide precursors (QMP-SOs) that enable proximity labeling and chemoproteomics to investigate superoxide redox biology. QMP-SOs are activated by superoxide to generate a reactive quinone methide intermediate that covalently tags nearby proteins. These probes exhibit high selectivity toward superoxide over other ROS, enabling protein labeling in superoxide-rich cellular compartments. QMP-SOs are compatible with fluorescence imaging and tandem mass tag (TMT)-based mass spectrometry, facilitating visualization of superoxide dynamics and identification of superoxide-regulated proteins. In menadione-treated HepG2 cells, QMP-SO-TMT profiling identified mitochondrial proteins including DJ-1 and DLDH as redox-sensitive targets, linking superoxide stress to cell survival and metabolism. The QMP-SO platform uniquely combines spatial proximity labeling with superoxide specificity, allowing previously inaccessible insights into dynamic and localized redox signaling. This invention is broadly applicable in studying superoxide-mediated processes and holds potential for therapeutic target discovery in diseases driven by oxidative stress, including cancer, aging, and neurodegeneration.

The described invention provides a pharmaceutical composition comprising a therapeutic amount of an educated mononuclear cell product, a process for preparing the educated mononuclear cell product, and a method for treating a disease characterized by lymphocyte autoreactivity. Mononuclear cells from a diseased subject are co-cultured with a viable population of adherent umbilical cord blood stem cells at at least 80% confluence to form an educated mononuclear cell product. A therapeutic amount of the educated mononuclear cell product is returned by infusion intravascularly to the subject. The therapeutic amount is effective to modulate autoreactivity in a T cell compartment of the subject and to reduce symptoms of the disease characterized by lymphocyte autoreactivity.

The present invention relates to a multi-compartment microfluidic device comprising a first cellular compartment (10) within which a target mucosa of a mucosal organ is reproduced and a second cellular compartment (40) comprising neurons (400), the second cellular compartment (40) being connected to the first cellular compartment (10) by a means forming a biological interface (50) to allow communication via neuronal connection (401) between the first cellular compartment (10) and the second cellular compartment (40). Figure 1

Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.