164 results about "Treated cell" patented technology

The invention provides an improved cell instance segmentation method and system based on YOLOv11, and the method comprises the steps: 1, constructing an improved YOLOv11 network, extracting the multi-scale features of an image, and generating feature maps of different scales; 2, introducing a multi-scale channel attention module into the backbone network, and introducing a new attention module into the multi-scale channel attention module to iteratively optimize initial fusion; step 3, integrating hierarchical feature maps generated by the encoder; 4, adding a scale sequence feature fusion module in the neck network, adding a triple feature encoder module, and fusing feature maps of different scales; step 5, adding a channel and position attention mechanism module in the neck network; and step 6, outputting a final segmentation result. The method is specially designed to be used for cell instance segmentation, and the accuracy and speed of processing cell images are improved by combining space and scale features.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

The invention provides a method and a system for processing a cell image based on a DistSegNet model. The method for detecting the cells based on the DistSegNet model image processing technology comprises the following steps: preprocessing a collected cell image; performing cell segmentation on the cell image based on a DistSegNet model image processing technology to generate a cell nucleus region; based on the segmented cell nucleus region, generating a background region, a foreground region and an edge region by adopting morphological operation; calculating the shortest Euclidean distance from each background region pixel to the nearest foreground region pixel, and segmenting the cell image by using a watershed algorithm; performing result optimization on the segmentation map corresponding to each cell instance label to obtain a filtered segmentation label map; and extracting the outer contour of the intra-nucleus region and the outer contour of the surrounding region of each cell from the segmentation mark graph, and storing the outer contours to a corresponding result file. According to the invention, the accuracy and accuracy of a WSI-level cell detection result can be improved.

The invention discloses an application of an MDH2 knockout system or inhibitor in reducing toxicity of T-2 toxin, and belongs to the technical field of biological agents. An MDH2 knockout IPEC-J2 cell line is constructed through a CRISPR / Cas9 gene editing system, and it is found that the cell activity of the cell line is remarkably improved under the condition of 20 ng / mLT-2 toxin concentration, the cell death rate is remarkably reduced, and the cell line has obvious resistance to T-2 toxin. The MDH2 inhibitor LW6 can also enhance the resistance of the cells to T-2 toxin when being used for treating the cells. Therefore, the toxicity of the T-2 toxin in the cell can be reduced by knocking out the MDH2 gene in the IPEC-J2 cell and inhibiting the activity of the MDH2, the tolerance of the cell to the T-2 toxin is improved, and a theoretical basis is provided for prevention and control of harm of the T-2 toxin to human and animals.

The invention provides a method and a system for processing a cell image based on a DistSeg model. The method for detecting the cells based on the DistSeg model image processing technology comprises the following steps: preprocessing a collected cell image; segmenting the cell image based on a DistSeg model image processing technology to generate a cell nucleus region; based on the segmented cell nucleus region, generating a background region, a foreground region and an edge region by adopting morphological operation; calculating the shortest Euclidean distance from each background region pixel to the nearest foreground region pixel, and segmenting the cell image by using a watershed algorithm; performing result optimization on the segmentation map corresponding to each cell instance label to obtain a filtered segmentation label map; and extracting the outer contour of the intra-nucleus region and the outer contour of the surrounding region of each cell from the segmentation mark graph, and storing the outer contours to a corresponding result file. According to the invention, the accuracy and accuracy of a WSI-level cell detection result can be improved.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention provides a red blood cell simulant as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) pretreatment: obtaining mammal whole blood and purifying to obtain red blood cells; (2) homogenization treatment: carrying out homogenization treatment on the red blood cells obtained in the step (1) by using a homogenization reagent; (3) cell repair treatment: carrying out cell repair treatment on the red blood cells obtained in the step (2) by using cell repair liquid; and (4) fixing treatment: fixing the red blood cells obtained in the step (3) by using a fixing agent. The preparation method disclosed by the invention is simple to operate and beneficial to large-scale production of products; the red blood cells in the prepared red blood cell simulant are good in homogeneity and stable in performance, the red blood cells in the red blood cell simulant are similar to red blood cells in human urine in shape, and the red blood cell simulant is used for preparing quality control substances for urine visible component analysis and can meet the requirements for accuracy and reliability of detection of the red blood cells in the urine.

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The present disclosure generally relates to, inter alia, a class of chimeric switch receptors containing an endodomain of an IL-9 receptor, engineered to modulate transcriptional regulation in a ligand-dependent manner. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating gene expression, modulating an activity of a cell, and / or for the treatment of various health conditions or diseases.

The present invention relates to a hybrid exosome formed by the fusion of a human cell-derived exosome and an artificial exosome. When an active ingredient labeled with a fluorescent material was attached or encapsulated in the hybrid exosome of the present invention and applied to cells, the active ingredient was observed in the cells. In addition, when the exosome was applied to skin tissue, fluorescence expression could also be observed in the dermal layer. Therefore, the hybrid exosome according to the present invention can be used as a novel drug delivery system.

A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.

The invention discloses a gray clustering characteristic distribution-based cell nucleoplasm segmentation method, which comprises the following steps of: 1) constructing a self-supervised network, and solving a cell intensity distribution diagram of a cell image; 2) determining cell candidate regions in the intensity maps of the cell distribution, the staining intensities of the intensity maps of the cell distribution are different, but the deepest part in the image must belong to a cell nucleoplasm region, so that the candidate regions of the cell nucleoplasm are solved by adopting an iterative search mode; and 3) carrying out accurate segmentation on the obtained candidate cell region to obtain a segmentation result of the cell nucleoplasm, and finally, correspondingly drawing a minimum circumscribed contour of the cell nucleoplasm in the original color map. The invention belongs to the technical field of image processing and biomedicine, and solves the problems that in the prior art, when a cell image is processed, the extraction part is inaccurate, impurities are easy to mix, and the detection precision is influenced.

The invention relates to the technical field of image recognition, and provides a multi-scale cervical cell automatic detection method and system based on a Cell-YOLOv8 model obtained after YOLOv8 improvement. The method comprises the following steps of: optimizing Bottleneck in a C2F module by adopting a CAFM to form a C2F-CAFM module, and then replacing the C2F module with the C2F-CAFM module in a feature extraction network; a CARAFE up-sampling operator is introduced into a feature fusion network, and then re-parameterization design is performed on the CARAFE up-sampling operator, so that the model can better detect and classify cervical cells, and the improved model effectively overcomes the limitations of more information loss and low feature learning efficiency when YOLOv8 processes fine cell features. While the real-time processing capability is maintained, the capability of capturing cervical cell characteristics is enhanced, so that the detection precision is improved.

The invention discloses a construction method of a tumor-associated meningeal metastasis model, and particularly relates to the technical field of biology. The method comprises the following steps: treating tumor cells according to a cell passage mode and centrifugal parameters, adding a serum-free culture medium, centrifuging again, adjusting the cell density by using a serum-free fresh culture medium, and sub-packaging to obtain a tumor cell suspension; the tumor cell suspension is injected into the L4-L5 intervertebral space of the mouse. After injection, the mouse resuscitates and normally diets; the growth of the tumor is monitored through a small animal living body imaging technology, and the dissected organ is subjected to in-vitro imaging to judge the invasion range of the tumor. The mouse tumor meningeal metastasis model is constructed in a simple, repeatable and efficient manner.

The invention discloses a cell image segmentation and function prediction method and device and computer equipment. The method comprises the steps of obtaining a to-be-processed cell image; inputting the to-be-processed cell image into the segmentation model for segmentation to obtain physical morphological features; inputting the physical morphological characteristics into a function prediction model to predict relevant parameters of the cell biological characteristics and relevant indexes of a set type of curative effect so as to obtain the parameters of the cell biological characteristics and the relevant indexes; and outputting physical morphological characteristics, cell biological characteristic parameters and related indexes. By implementing the method provided by the invention, the undyed bright field cell image can be effectively processed, the cell morphology and function are associated, the method is specially used for segmenting the mesenchymal stem cells, and the problem of instance segmentation under the condition of cell overlapping is solved.

The invention is applicable to the technical field of biological medicines, and provides a frozen cell as well as a preparation method and application thereof. According to the invention, frozen human umbilical cord mesenchymal stem cells (CK-MSCs) are adopted as a carrier of mitoxantrone (MTX), so that accurate delivery and pH-sensitive controlled release of drugs at tumor sites are realized, the treatment effect is remarkably improved, and the side effects of traditional chemotherapy are greatly reduced. The freezing treatment enables the human umbilical cord mesenchymal stem cells to lose the multiplication capacity, so that the tumor formation risk is eliminated, and the treatment safety is ensured. By adjusting the immune response in the tumor microenvironment, the CK-MTX not only directly inhibits the tumor, but also enhances the anti-tumor immune defense of the body. The freezing treatment method disclosed by the invention can be applied to other types of cells, and the frozen cells can be compatible with other medicines, so that revolutionary breakthrough is brought to tumor treatment, and a new way is opened up for treatment of other diseases.

An iPS cell production system according to an embodiment of the present invention includes: a cartridge provided with a flow path; and a production apparatus for producing iPS cells in the cartridge. The production apparatus has a liquid feeding mechanism, a culture device, and a control device. The liquid feeding mechanism applies, to the flow path in the cartridge, energy for feeding a cell liquid that contains target cells from a donor and a reagent that is used for processing the cell liquid. The culture device controls the culturing environment in the cartridge in order to culture, in the cartridge, iPS cells established in the cartridge through the action, on the target cells, of a reprogramming factor for reprogramming the target cells. The control device controls liquid feeding operation by the liquid feeding mechanism and environment control operation by the culture device in order to produce iPS cells from the target cells.

The invention discloses an application of a hypoxia pretreated extracellular vesicle / miR-27b-3p in the treatment of ischemic stroke. The research proves that both the neuron-derived EV and H-EV can promote in-vivo and in-vitro MCAO repair. The two vesicles secreted by neuronal cells can significantly influence phenotypic change and apoptosis of astrocytes, wherein the effect of H-EV is more obvious than that of EV. Besides, the research result shows that H-EV secreted by neurons can regulate a PI3K / AKT signal channel by transmitting miR-27b-3p, so that astrocytes are promoted to be converted from the A1 phenotype to the A2 phenotype. The research of the invention provides a new therapeutic target and strategy for treating ischemic stroke.

To detect an inclination of a cartridge, the cartridge being configured to internally perform processing on a cell.SOLUTION: A sample processing apparatus according to the present embodiment includes an apparatus body. In the apparatus body, a cartridge for internally performing processing on a cell is installed, and the apparatus body controls liquid feeding in the cartridge and / or adjusts an environment in the cartridge. The apparatus body includes a detector configured to detect an inclination of the cartridge with respect to a horizontal plane and / or the apparatus body.SELECTED DRAWING: Figure 5

The present invention discloses an in vitro preparation method of human mesenchymal stem cells. The method comprises the following steps: using MSC culture medium supplemented with chemical small molecules to induce iPSC-derived MSCs; after the iPSC cell confluence reaches 40%, the culture medium containing chemical small molecules is replaced; after 8-10 days of induction, the cells are collected and passaged; and the cells are cultured to the P1 generation, showing uniform fibroblast-like, spindle-shaped or fusiform cells. The prepared hiPSC-MSCs meet the relevant phenotypes of mesenchymal stem cells, wherein the positive rates of CD73, CD90 and CD105 are higher than 95%, and the cells have immunomodulatory activity.

The present invention relates to a method for performing a biological process on a liquid immune cell or an initial cell culture to obtain a treated cell culture, where the treated cell culture is intended for autologous or allogeneic cell therapy, where the biological process is performed on an integrated biological treatment system (1), wherein the biological process comprises a series of treatment steps wherein the treatment steps each comprise at least one operation wherein the biological process system (1) comprises a base structure (3) and a preconfigurable cartridge (4) wherein the biological process system (1) performs the operation of the biological process via interaction of the base structure (3) with the cartridge (4), wherein the cartridge (4) and the base structure (3) comprise a matching standardized interface (6) for interaction of the base structure (3) with a corresponding cartridge (4), wherein the biological process system (1) performs at least two operations of the biological process inside at least two differently preconfigured cartridges (4) by interaction of the base structure (3) with the cartridges (4) via the same base structure interface (7) and / or the same base structure interface (7) and a mating cartridge interface (8).

The present invention provides: a single-strand form polynucleotide for editing that is capable of modifying a target site in double-strand genomic DNA and that includes, as a structural unit, a nucleotide analog with a high affinity for DNA; a genome editing kit that includes said polynucleotide for editing; a genome editing kit that includes said polynucleotide for editing and a polynucleotide for promoting editing or an expression vector thereof; a method for modifying a target site in double-strand genomic DNA of a cell or a non-human organism, the method comprising a step for using the genome editing kit to treat a cell or an organism; 10 and a method for producing a cell or an organism in which a target site in double-strand genomic DNA is modified, the method comprising said step.

In one aspect, a method of treating an NK cell to induce a gene edit. The method may include contacting an NK cell with a transfection composition including poly-L-glutamic acid (y- PGA), a single-stranded oligo deoxynucleotide (ssODN) enhancer, or a combination thereof; contacting the NK cell with an editing composition including: a DNA-PK inhibitor, and an HD AC inhibitor; electroporating the NK cell; and contacting the NK cell with a recovery composition including DNase I. In another aspect, a method of preparing an engineered NK cell comprising a fluorescent reporter at an endogenous locus. In another aspect, a method of treating an NK cell to introduce an exogenous nucleic acid.

Methods for analyzing cell or tissue cultures are described. In an embodiment, the method includes: receiving cellular or tissue data, wherein: the cell or tissue cultures are organized by organizational structures in the device; and the cellular or tissue data is correlated with at least one of: a physiological component of the cell or tissue cultures, and a position of the cell or tissue cultures in the device; identifying at least one region of interest in the cellular or tissue data corresponding to at least one of: the physiology of the cell or tissue cultures in the device and at least one of the organizational structures in the device; and processing the region of interest in the cellular or tissue data to measure at least one parameter relating to the cell or tissue cultures. Corresponding systems and computer-readable media are also described.

To reduce complication in incorporating a reagent into a cartridge for cell treatment.SOLUTION: A cartridge according to an embodiment is a cartridge for performing processing of cells therein, and includes a flow path through which a reagent used for the processing flows, a connection portion for connecting a reagent container containing the reagent to the flow path, and an opening / closing portion for connecting the reagent container to the connection portion and installing the reagent container in the cartridge.SELECTED DRAWING: Figure 4

The application discloses an animal cell adaptive tracking method and system based on deep learning, and belongs to the technical field of cell image analysis; a YOLOv10 deep learning model is combined with a Btrack adaptive tracking algorithm, animal cell samples are first dyed and microimage collection is carried out, a cell detection model is trained after image preprocessing, data labeling and augmentation, the model is converted into an ONNX format to realize efficient inference; then, Kalman filtering prediction and three-round Hungarian algorithm matching are carried out to complete cell adaptive tracking, the problems of cell occlusion, division, overlap and loss of tracking can be effectively handled, and stable and continuous cell motion trajectories are generated; the application can be deployed without GPU, has strong generalization ability, high detection and tracking precision, is suitable for scenes such as microecological research, clinical sample testing, drug effect evaluation and cell behavior analysis, and can significantly improve the automation level and stability of animal cell detection, counting and dynamic tracking.

Disclosed are methods for selecting, expanding,and / or enriching cells that are resistant to an immunosuppressive agent (e.g., MTX), and the method comprising treating the cells with the immunosuppressive agent and human platelet lysate (HPL). In some embodiments, the cells (e.g., CAR-T cells) can be used for cell therapy.

The present disclosure pertains to methods for handling cells and / or cell clusters without the use of centrifugation. In one aspect, described is a method of separating a liquid from a composition comprising the liquid and cell clusters. Therein, the liquid of the composition is passed through a filter disposed within a pipette so as to separate the liquid from the cell clusters.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The invention belongs to the technical field of algae physiology, and particularly discloses a method for efficiently extracting microalgae chlorophyll, which comprises the following steps: a, taking an algae liquid sample, centrifuging, discarding supernatant, and adding 90% ethanol solution for resuspension; b, adding quartz sand into the resuspension, carrying out cell homogenization treatment under the condition of 60 Hz, repeating the cell homogenization treatment for 4 times, 45 seconds each time, and 5 seconds each time; and c, heating the sample in a constant-temperature water bath at 60 DEG C for 7 minutes, centrifuging, and collecting supernate. By optimizing the ethanol concentration, the cell homogenization time, the cell homogenization material and the hot bath time, the cell homogenization technology of combining an ethanol method with quartz sand broken algae cells is improved, the chlorophyll extraction efficiency is effectively improved, the stability is good, and large-scale application is easy. Compared with an acetone method, an ethanol method and an ethanol-steel ball method, the microalgae chlorophyll extraction method provided by the invention has obvious advantages.