46 results about "Developmental genes" patented technology

The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding proteins that are associated with neurodevelopmental disorders. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. Also provided are methods of using the genetically modified animals or cells disclosed herein to screen agents for toxicity and other effects.

The invention discloses a gene for controlling male reproduction and development of rice and application of the gene. The gene has a nucleotide sequence represented by SEQ ID NO.1, and a protein encoded by the gene has an amino acid sequence represented by SEQ ID NO.2. By targeting a LOC_Os08g20730 gene design target by virtue of a CRISPR / Cas9 gene editing system, a receptor is a middle flower 11, and a targeted T0 generation phenotype are shown in the figures 1, 2, 3 and 4 in the description; and sequencing analysis shows that anther development abnormality occurs in a homozygously mutated plant or a biallelically mutated plant and pollen does not exist. The analysis of a homozygously mutated T1 generation shows that the separation proportion of fertile plants to sterile plants is 3 to 1. The integration shows that the gene is capable of regulating and controlling the reproduction and development of rice, once the gene in the rice is damaged, the anther development abnormality occurs, and non-pollen sterility occurs. According to the obtained cell nucleus gene for controlling the reproduction and development of the rice, an intelligent sterile line can be created by virtue of a molecular measure, and excellent parents in the production are improved into excellent sterile lines, so that the heterosis utilization is expanded.

The invention discloses a plant anther-specific promoter and application thereof. The promoter is 179 to 1174 bp in length at least containing the 996-1174th nucleotide sequence at the 5' end of sequence 1 in the sequence listing at the 3' end and extending to the 5' end according to the nucleotide arrangement of sequence 1 deoxynucleotide fragments. The anther-specific promoter of the present invention has great application prospects in the functional analysis and identification of plant anther growth and development genes, the establishment of artificial male sterile lines, the prevention of plant transgene drift or escape, and the extension of shelf life of flowers.

The invention discloses rice spikelet development regulation protein.The amino acid sequence of the protein is as shown in (A) Seq ID No: 2; or the protein is formed as shown in (B) formed in the mode that one or more amino acids are added to and / or substituted for in and / or deleted from the amino acid sequence defined by (A), has a function of regulating rice spikelet development and is derived by the (A).The invention further discloses genes for encoding the protein, such as the gene as shown in SEQ ID No:1 and the gene as shown in SEQ ID No:3.; the invention further discloses a recombinant vector and a transformant which contain the genes, a method for regulating gramineous plant spikelet development through gene transformed cells and application of the protein, the genes, the recombinant vector and the transformant to improvement of the yield and quality of gramineous plants.According to the rice spikelet development regulation protein, the encoding genes MSi thereof and application, a map-based cloning technology is utilized to separate the MS1 genes influencing rice spikelet development, the functions of the genes are identified through a genetically modified complementary experiment, and the genes can be utilized to research the molecular mechanism of rice glumes and seed size so that the yield of gramineous plants can be raised, and quality of gramineous plant can be improved.

The invention provides a tomato protein SLMBP21 and a tomato gene SLMBP21 for coding the protein, wherein the nucleotide sequence of the gene is shown in SEQ ID No.1. The invention has the advantages that the tomato gene SLMBP21 belongs to a tomato abscission zone developmental gene in an MADS-box gene family, is capable of inhibiting the expression of the gene SLMBP21 so as to obviously influence the development of a tomato abscission zone to produce a transgenic plant with no tomato abscission zone or an incompletely developed abscission zone. The tomato protein SLMBP21 can be used for solving the problems of blossom drop and fruit drop of various crops, vegetables, fruits and flowers and the problem of industrial picking of tomatoes; moreover, different form the situation that the development of inflorescence in a JOINTLESS mutant is affected, the development of inflorescence in the SLMBP21transgenic plant is not affected, which means that the gene SLMBP21 has higher application value.

The invention discloses a rice glume development gene promoter p-TRI1 and application thereof. The promoter provided by the invention comes from ordinary cultivated rice O. sativa L. 93-11 which belongs to Oryza species, and the promoter is a DNA molecule of the following 1), 2) or 3): 1) a DNA molecule shown in Sequence 1 in a sequence table; 2) a DNA molecule which crosses with a DNA sequence limited by 1) or 2) under strict condition and has the promoter function; and 3) a DNA molecule which has more than 90 percent of homology with the DNA sequence limited by 1) or 2) and has the promoter function. The discovery of the promoter and the elucidation of the promoter function have important theoretical and practical significance for studying flower organ development mechanism of rice, especially the glume development mechanism and for breeding rice varieties of specific grain types. The promoter has important theoretical and practical significance for studying the molecular mechanism of rice glume development and for rice grain type molecular breeding. The promoter has wide application and market prospect in the field of agricultural.

The invention discloses a method for transforming a cucumis melo female line through a high-throughout molecular marker and a special primer thereof. The special primer is a KASP primer set used for detecting cucumis-melo sex genes CmACS7 and CmWIP1, and the specific nucleotide sequence of the primer is the first sequence to the sixth sequence in a sequence table. The types of the cucumis-melo sex genes are detected through a high-throughout molecular mark system based on a PCR SNPLine platform provided by the method, the operation process is full automatic, and human errors are reduced; the analysis throughout is high, and the method and the special primer are quite suitable for detecting a large number of samples at the same time. According to the method and the special primer, the high-throughout detection molecular marker is designed based on the sequences of the cucumis-melo instaminate flower development A / a genes and the G / g genes and applied to transforming of the cucumis-melo female-line genes, time cost and manual cost can be greatly saved, the molecular-marker-assisted selection seed breeding efficiency can be improved, and transformation of cucumis-melo female characters to excellent key inbred lines is accelerated.

The invention discloses a gene OsBEAR1 for adjusting and controlling growth and development of coleoptiles of oryza sativa, and an application of the gene OsBEAR1. The MSU ID of the gene is ''LOC_Os11g15210'', and the gene is used for coding an amino acid sequence as shown in SEQ ID NO:3. By a gene editing method, the gene mutant is knocked out in Oryza sativa L. cv. Nipponbare, growth and development of the coleoptiles of oryza sativa seedlings after OsBEAR1 knockout are accelerated, and the length of the coleoptiles of the oryza sativa seedlings after OsBEAR1 knockout is notably longer thanthat of coleoptiles of receptor oryza sativa seedlings. The elongation of the coleoptiles facilitates sprouting of oryza sativa and direct sowing in the fields. According to the gene OsBEAR1 disclosedby the invention, a base is provided for reformation and breeding of the varieties of oryza sativa suitable for direct sowing in the fields.

The invention relates to a poplar leaf type developmental control gene and application thereof, which belong to the technical field of plant genetic engineering and the biological field. A nucleotidesequence of the poplar leaf type development control gene PagKNAT2 / 6b is as shown in a sequence 1 in a sequence table; an amino acid sequence, expressing protein, of PagKNAT2 / 6b is as shown in sequence 2 in the sequence table. The PagKNAT2 / 6b gene is shifted into 84k poplar, and the transgenic polar which excessively expresses PagKNAT2 / 6b is compared with a wild type, so that an obvious rolled andfolded leaf type appears, and therefore, the PagKNAT2 / 6b gene is the key of controlling poplar leaf development, and has an important application value in the woods genetic engineering field and theclonal forestry field.

The invention discloses a regulatory gene PdeMIXTA02 for initial development of floc of Poplus deltoides and application of the regulatory gene PdeMIXTA02, and belongs to the technical field of plantgenetic engineering. Through gene sequence evolution analysis and gene expression analysis at different time points before and after initial development of poplar floc and combination of verificationof the genetic transformation function of Arabidopsis thaliana, the gene PdeMIXTA02 for control on initial development of poplar floc is disclosed firstly. The gene belongs to a family of MIXTA transcription factors, it is shown through sequence evolution analysis that the gene and key genes GhMML3 and GhMML4 for regulating the initial development of cotton wadding are in the same branch of MIXTAsubclasses, and the gene only has specific expression within 5 days after pollination of female floc of Poplus deltoides, the amount of epidermal hair can be increased significantly through excessiveexpression in Arabidopsis thaliana, and bump differentiation of epidermal cells of Arabidopsis thaliana into epidermal hair is promoted; and it is confirmed through the verification that the gene PdeMIXTA02 for regulating the initial development of poplar floc can be applied to breeding of new poplar varieties without fly floc.

The invention relates to a gene for controlling early development of rice chloroplast and a detection method and an application of the gene. The invention discloses a gene for controlling early development of rice chloroplast, wherein the gene has a nucleotide sequence as shown in SEQIDNo.1, and protein encoded by the gene has an amino acid sequence as shown in SEQIDNo.2. By utilizing designed specific PCR (Polymerase Chain Reaction) primers as shown in SEQIDNo.4 and No.5, rice plants containing the gene can be quickly detected after PCR amplification and ScaI enzyme digestion for appraisal are carried out on DNA (Deoxyribose Nucleic Acid) extracted from rice, and the detection method is high in detection accuracy, easy to operate and low in cost. Early rice plant yellowing can be caused when the gene in rice is damaged. The gene for controlling early development of rice chloroplast obtained by the invention can be applied to hybrid rice seed production, and the purity of a hybrid rice sterile line and the hybrid rice hybridized by the hybrid rice sterile line can be improved.

The invention relates to application of a rice reproductive development gene MMD2 and a method for restoring male sterility of rice in the technical field of rice breeding. A sequence of the MMD2 gene is shown in SEQ ID NO.1, and an amino acid sequence encoded by the MMD2 gene is shown in SEQ ID NO.2. The application comprises the steps of enabling rice male reproductive development to be abnormal through gene variation or expression suppression, obtaining a rice male sterile line and producing hybrid seeds by adopting the rice male sterile line. The method for restoring male sterility caused by loss of functions of the MMD2 gene comprises the steps of amplifying the MMD2 gene by virtue of a primer, constructing a vector and transforming a mutant by adopting a genetic transformation method, wherein the mutant can be restored to a wild type phenotype. The obtained rice mutant has no abnormality in a nutrition growth stage and is abnormal in reproductive growth, so that complete infertility is caused; and when the rice mutant is applied to cross breeding, castration on a female parent can be eliminated, the production efficiency is greatly improved, and the labour cost is reduced, so that the rice mutant has important application values in agricultural production.

The invention discloses a wheat leaf developmental gene TaWRKY76 and an application thereof. The nucleotide sequence of the gene complementary deoxyribonucleic acid (cDNA) is shown in SEQ ID No.1. The invention further discloses an application of the gene TaWRKY76 in culture of plants with curled leaves. Experiments prove that the wheat TaWRKY76 can be introduced into plant cells, so that the plants can obtain a phenotype of curled leaves, advantages are provided for improving photosynthesis, and the gene can be widely applied to culture of high-yield crops varieties.

The invention describes a gene for encoding DExD / H-box RNA helicase protein in corn and an amino acid sequence of the protein encoded by the gene, and the nucleotide sequence and the protein encoded by the gene have the functions of regulating corn kernel development and endosperm structure and components; and a functional marker of the gene is developed, can be used for corn quality improvement, and has great application and economic values.

The invention belongs to the field of plant genetic engineering and particularly relates to control on isolation and cloning and function verification of a rice glumes developmental gene AH1 and application of the rice glumes developmental gene AH1 in rice breeding. The invention discloses the rice glumes developmental gene AH1. A nucleotide sequence of the gene is as shown in SEQ ID NO:2. The invention also simultaneously discloses a protein coded by the rice glumes developmental gene AH1. An nucleotide sequence of the protein is as shown in SEQ ID NO:4. By adopting the rice glumes developmental gene AH1, lemmas of glumes are bent, glumelle is degenerated, and the entire glumes is of a crescent shape; the rice glumes developmental gene AH1 can be used for improvement on yield and geneticquality of gramineous plants.

Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The invention discloses an OsZFP gene and application of OsZFP gene protein to rice lateral root growth and development regulation and control. Through a yeast two-hybrid system (in vivo) and a GST pull down (in vitro) technology, downstream target protein OsZFP achieving the mutual action with an important functional protein OsCYP2 for rice lateral root growth and development regulation and control is screened and verified; and an RNAi technology is used for proving that the gene takes part in the rice lateral root growth and development. The invention aims at exploring a novel rice root system growth and development regulation and control gene, enriching a rice lateral root growth and development gene regulation and control network and a relevant signal transduction path, and providing a novel idea for the rice lateral root development gene function study. Meanwhile, important significance is realized on disclosing the rice root system growth and development mechanism and improving the rice productivity.

The invention relates to a method for separating and regulating peanut embryonic development genes, and belongs to the field of functional genomics. The AhHsfa4A gene sequence is as shown in SEQ ID NO:1; the important gene AhHsfa4A closely related to peanut early embryonic cell programmed cell death induced by calcium is obtained through screening; and a complete sequence of the AhHsfa4A gene is obtained from a constructed whole-tissue full-length library through modified RACE technology. The invention establishes a method for high-efficient screening of calcium deficiency-induced peanut early embryo important genes (SSHaLL); an important gene AhHsfa4A which can cause peanut early embryonic cell programmed cell death is screened by the method, and the expression of the gene is identified. The method of the invention lays a good foundation for high-efficient screening of important differential expression genes of peanut embryonic development, and for further demonstration of the molecular mechanism of peanut embryonic development.

The invention provides a tomato protein SLMBP21 and a tomato gene SLMBP21 for coding the protein, wherein the nucleotide sequence of the gene is shown in SEQ ID No.1. The invention has the advantages that the tomato gene SLMBP21 belongs to a tomato abscission zone developmental gene in an MADS-box gene family, is capable of inhibiting the expression of the gene SLMBP21 so as to obviously influence the development of a tomato abscission zone to produce a transgenic plant with no tomato abscission zone or an incompletely developed abscission zone. The tomato protein SLMBP21 can be used for solving the problems of blossom drop and fruit drop of various crops, vegetables, fruits and flowers and the problem of industrial picking of tomatoes; moreover, different form the situation that the development of inflorescence in a JOINTLESS mutant is affected, the development of inflorescence in the SLMBP21transgenic plant is not affected, which means that the gene SLMBP21 has higher application value.

Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

The invention discloses a gene for controlling male reproduction and development of rice and application of the gene. The gene has a nucleotide sequence represented by SEQ ID NO.1, and a protein encoded by the gene has an amino acid sequence represented by SEQ ID NO.2. By targeting a LOC_Os08g20730 gene design target by virtue of a CRISPR / Cas9 gene editing system, a receptor is a middle flower 11, and a targeted T0 generation phenotype are shown in the figures 1, 2, 3 and 4 in the description; and sequencing analysis shows that anther development abnormality occurs in a homozygously mutated plant or a biallelically mutated plant and pollen does not exist. The analysis of a homozygously mutated T1 generation shows that the separation proportion of fertile plants to sterile plants is 3 to 1. The integration shows that the gene is capable of regulating and controlling the reproduction and development of rice, once the gene in the rice is damaged, the anther development abnormality occurs, and non-pollen sterility occurs. According to the obtained cell nucleus gene for controlling the reproduction and development of the rice, an intelligent sterile line can be created by virtue of a molecular measure, and excellent parents in the production are improved into excellent sterile lines, so that the heterosis utilization is expanded.

The invention discloses a rice anther development regulation gene EDT1 and application thereof. The DNA sequence of the rice anther development regulation gene EDT1 is shown as SEQ ID NO.1. The rice anther development regulation gene EDT1 is obtained by researching a male sterility mutant edt1. The rice anther development regulation gene EDT1 can provide excellent provenance to cultivate high-quality rice varieties with high yield, high stress resistance and wide adaptability and has important application value in plant breeding.

The invention relates to a method for isolating and regulating peanut embryo development genes, which belongs to the field of functional genomics. AhHsfa4A The gene sequence is shown as SEQ ID NO: 1, which will be screened to obtain the closely related important genes that are induced by calcium to cause programmed death of peanut early germ cells AhHsfa4A , and obtained from the constructed whole-tissue full-length library by improved RACE technology AhHsfa4A The full sequence of the gene. The present invention establishes a method (SSHaLL) for efficiently screening important genes of peanut early embryos induced by calcium deficiency, and uses this method to screen an important gene that causes programmed death of early peanut embryos AhHsfa4A And its expression was identified. The invention has laid a good foundation for efficient screening of important differentially expressed genes in peanut embryo development, thereby further clarifying the molecular mechanism of peanut embryo development.

The invention relates to a poplar leaf type developmental control gene and application thereof, which belong to the technical field of plant genetic engineering and the biological field. A nucleotidesequence of the poplar leaf type development control gene PagKNAT2 / 6b is as shown in a sequence 1 in a sequence table; an amino acid sequence, expressing protein, of PagKNAT2 / 6b is as shown in sequence 2 in the sequence table. The PagKNAT2 / 6b gene is shifted into 84k poplar, and the transgenic polar which excessively expresses PagKNAT2 / 6b is compared with a wild type, so that an obvious rolled andfolded leaf type appears, and therefore, the PagKNAT2 / 6b gene is the key of controlling poplar leaf development, and has an important application value in the woods genetic engineering field and theclonal forestry field.

The invention discloses a wheat leaf developmental gene TaWRKY76 and an application thereof. The nucleotide sequence of the gene complementary deoxyribonucleic acid (cDNA) is shown in SEQ ID No.1. The invention further discloses an application of the gene TaWRKY76 in culture of plants with curled leaves. Experiments prove that the wheat TaWRKY76 can be introduced into plant cells, so that the plants can obtain a phenotype of curled leaves, advantages are provided for improving photosynthesis, and the gene can be widely applied to culture of high-yield crops varieties.

The invention discloses a plant anther specific promoter and the application. The promoter is the oligodeoxynucleotide with the length of 179bp to 1174bp. The 3'end of the promoter at least contains 996 site to 1174 site nucleic acid sequence at 5' end of sequence 1 in the self-sequence table, which extends along the nucleic acid arrangement mode of the sequence 1. The anther specific promoter has important application prospect for the function analysis and discrimination of the growth and development gene of the plant anther, the construction of artificial sterile system, the prevention of plant transgenic drift or escape and the extension of shelf life of flowers etc.

The invention discloses a gene regulating the growth and development of rice coleoptile OsBEAR1 For its application, the MSU ID of the gene is "LOC_Os11g15210", encoding the amino acid sequence shown in SEQ ID NO:3. Knockout the gene mutation in Nipponbare by gene editing method, knockout OsBEAR1 The growth and development of the coleoptiles of the rice seedlings were accelerated and were significantly longer than those of the recipient rice seedlings. The growth of the coleoptile is beneficial to rice germination and field direct seeding, and the invention provides a basis for the transformation and cultivation of rice varieties suitable for field direct direct seeding.