17 results about "Developmental genes" patented technology

The application discloses a grape seed development gene VvPHERES1 and application thereof. The nucleotide sequence of the development gene VvPHERES1 is shown as SEQ ID NO:1. The amino acid sequence of a Type I MADS transcription factor is shown as SEQ ID NO:2. The application clones a grape seed development related gene VvPHERES1 for the first time and studies the gene function. A pCAMBIA2300-VvPHERES1 overexpression vector and a heterologous Micro-Tom tomato plant are constructed. By comparing the changes of seed traits in the overexpression transgenic tomato and the wild type plant, the result shows that the seed quantity in the overexpression transgenic tomato is obviously increased. The application provides a grape seed development related gene, and provides a basis for breeding of seedless grape new varieties.

The invention relates to the field of plant genetic engineering, and particularly discloses a soapberry SmCYCD3; 2 gene and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the sequence of the protein coded by the gene is shown as SEQ ID NO: 2. Experiments show that the gene has high specificity expression in female flowers of soapberry. By heterologous overexpression of the gene in arabidopsis thaliana, typical'female promotion and male inhibition 'phenotypes such as pistil elongation, stamen number reduction and shortening, pollen viability reduction and small fruit pods of transgenic plants can be caused. Molecular mechanism studies show that: SmCYCD3; 2, performing functions by up-regulating expression of cell cycle related genes and pistil development genes AtCRC and down-regulating expression of stamen development key genes at the same time; the invention provides a key gene resource and a theoretical basis for directionally regulating and controlling the sex ratio of plant flowers through a molecular breeding means and improving the fruit yield of woody economic tree species.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.

The invention relates to a bemisia tabaci nymph development gene BtPri39 and application thereof, and belongs to the field of biological control. The gene BtPri39 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtPri39 is an efficient RNAi lethal target with potential. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa which is the natural enemy of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a very good application prospect.

The application belongs to the technical field of corn genetic breeding, and specifically discloses a corn kernel development gene ZmCHR24 and application thereof. ZmCHR24 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application obtains a mutant material with loss of gene function through EMS mutagenesis and Mu transposon insertion technology. ZmCHR24 The phenotype analysis result shows that, compared with the wild type, ZmCHR24 the kernel of the mutant with loss of gene function presents severe development defects, and specifically, the kernel is only pericarp with no embryo and endosperm. ZmCHR24 The above result shows that the gene is a key gene for regulating corn kernel development. The application provides an important theoretical basis and gene resource for genetic improvement and molecular design breeding of the yield trait of corn kernels.

This invention discloses a rice grain-per-panicle number gene, OsTT2, and its applications, belonging to the fields of plant genetic engineering and molecular breeding technology. Through CRISPR / Cas9 gene editing experiments, this invention demonstrates that knocking out the OsTT2 gene significantly reduces the grain-per-panicle number in rice. It also elucidates the molecular mechanism by which OsTT2 influences grain-per-panicle number by directly binding to and activating the co-regulatory factor OsMED15a promoter, thereby regulating downstream grain shape development genes. Furthermore, this invention identifies for the first time a 331 bp fragment duplication variant (SD2) in the OsTT2 gene promoter region associated with the high grain-per-panicle number trait, and based on this, develops molecular markers for selection assistance and corresponding rice breeding methods. This invention provides new key gene resources and precision breeding tools for high-yield rice breeding.

The application discloses a poplar root hair development gene PsbHLH89 and application thereof, and belongs to the technical field of plant genetic engineering; the application provides the poplar root hair development gene PsbHLH89 and the coding protein thereof on one hand, and provides the application of the poplar root hair development gene PsbHLH89 on the other hand. The application provides an important and possibly universal root hair development gene resource, the poplar root hair development gene provides material for later related research, and lays a foundation for plant root hair development research.

The application discloses a growth and development gene of Gossypium hirsutum and application thereof. The gene Gohir.A08G240900 related to growth and development is mined out by reverse transcription of RNA of Gossypium hirsutum, a nucleotide sequence of the gene is shown as SEQ ID No. 1, the gene can affect plant height of Gossypium hirsutum, and the expression amount of the gene is more in Gossypium hirsutum with high plant height, and the expression amount of the gene is significantly reduced in Gossypium hirsutum with short plant height. Meanwhile, through a virus-induced gene silencing (VIGS) experiment, it is found that the gene Gohir.A08G240900 causes growth and development of a plant to be damaged after silencing, thereby affecting growth of the plant, so that a phenotype of plant height being significantly short is shown, and it is shown that the gene Gohir.A08G240900 is a potential target for regulating plant height, and a new genetic basis for improving plant height of Gossypium hirsutum is provided.

The invention discloses application of an RFLNA gene in regulating and controlling proliferation and development of pig thoracic vertebra growth plate cartilage cells, and belongs to the technical field of pig breeding. The invention finds that the proliferation and development of the pig thoracic vertebra growth plate cartilage cells can be regulated and controlled by regulating and controlling the expression of the RFLNA gene. The RFLNA gene can be applied to genetic breeding of pig body characters, acceleration of breeding progress, improvement of meat production rate or manufacturing of small pig experimental animal models, and has great economic value and scientific research value.

This invention discloses an indicator gene for the dragon fruit VIGS system. HpbHLH48 This invention relates to the field of molecular biology. The TRV2-HpbHLH48 recombinant vector was constructed and applied to the VIGS experiment on dragon fruit. Results showed that after injection of the empty TRV1-TRV2 vector, the dragon fruit peel in the injection area only developed scabs and normally changed color to red; however, after injection of the TRV2-HpbHLH48 recombinant vector, the dragon fruit peel in the injection area remained green and did not change color. Simultaneously, the dragon fruit peel area injected with the TRV2-HpbHLH48 recombinant vector showed... HpbHLH48 The gene expression level was significantly lower than that of the empty TRV1-TRV2 vector. This indicates that... HpbHLH48 This invention can serve as an indicator gene for the VIGS system in dragon fruit. It provides a technological foundation for future functional verification of genes involved in dragon fruit development.

This invention provides a rice dominant nuclear male sterility expression vector constructed based on the EAT1 gene and its application. The expression vector has... Ubiquitin Promoters and genes for male flower development in rice EAT1 It is formed by operable connections. Specific construction methods include: obtaining rice cDNA and using it as a template, employing... EAT1 Gene-specific primer amplification EAT1 Genes were extracted to obtain PCR product fragments; these PCR product fragments were then ligated into genes containing... Ubiquitin The expression vector is obtained by placing the promoter in a skeletal vector. Transgenic plants obtained using this expression vector exhibit dominant nuclear male sterility, and dominant nuclear male sterile rice lines can be created through hybridization. Recurrent selection breeding using these dominant nuclear male sterile lines can rapidly eliminate non-target materials, efficiently aggregate multiple superior traits and genes, and does not contain transgenic components, effectively improving breeding efficiency and safety.

The present invention belongs to the technical field of plant molecular breeding, and in particular to a cotton high temperature response gene GhMucin17-like, an encoded protein and its application. The present invention utilizes a natural population of 510 upland cotton germplasms, collects anther phenotypes under normal and high temperature conditions for many years at multiple points, evaluates the anther cracking heat resistance index, and through genome-wide association analysis, LD-block analysis and haplotype analysis, finds the cotton high temperature response gene GhMucin17-like, whose gene sequence is shown in SEQ ID No.4. By constructing a vector for knocking out the gene, a mutant plant is obtained, and the results of the embodiment after editing and testing the plant show that the GhMucin17-like gene significantly affects the cracking of cotton anthers and even the development of anthers, and the GhMucin17-like gene is of great significance in guiding cotton germplasm improvement.

The invention discloses a rice chalkiness related gene OsPRS4 as well as an encoded protein and application thereof, and the gene OsPRS4 provided by the invention is a DNA molecule as shown in the following 1), 2), 3) or 4): 1) a DNA molecule as shown in SEQ ID NO.1; 2) a DNA molecule as shown in SEQ ID NO. 2; 3) a DNA molecule which is hybridized with the DNA sequence limited by 1) or 2) under strict conditions and is used for coding the protein; and 4) a DNA molecule which has more than 90% of homology with the DNA sequence limited by 1) or 2) or 3) and is used for coding the plant endosperm development related protein. The invention also provides a protein coded by the gene, the protein affects plant endosperm development, and transgenic plants with normal endosperm development can be cultivated by introducing the coding gene of the protein into plants with abnormal endosperm development. The coding gene of the protein is guided in wild plants to interfere, so that endosperm powder plants can be obtained. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses DNA demethylase IbROS1 related to sweet potato root tuber development and application, and particularly relates to the field of plant genetic engineering and molecular breeding, the nucleotide sequence of the IbROS1 gene is as shown in SEQ ID No.1, and the sequence of protein coded by the IbROS1 gene is as shown in SEQ ID No.2. According to the invention, an IbROS1 gene is cloned, a plant overexpression vector of the IbROS1 gene is constructed, and an optimized agrobacterium-mediated method is utilized to transform and cultivate sweet potatoes, so that an IbROS1 overexpressed transgenic sweet potato strain is obtained; phenotypic analysis finds that overexpression of IbROS1 can significantly reduce the methylation level of sweet potato genome DNA, and finds that IbROS1 strongly inhibits formation and expansion of tuberous roots, resulting in sharp drop of root weight, reduction of starch content, increase of lignin content and change of related developmental gene expression profiles; the key negative regulation effect of the IbROS1 in sweet potato root tuber development is disclosed, a new gene resource and theoretical basis are provided for accurately regulating the yield and quality of sweet potatoes through an epigenetic means, and the IbROS1 has an important application prospect in sweet potato molecular breeding.

The invention relates to a gene ZmESKIMO1 influencing corn root development and application thereof, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene ZmESKIMO1 is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the gene is shown as SEQ ID NO.2. The gene is capable of regulating the growth and development of plants, and overexpression of the gene ZmESKIMO1 enables primary root systems of the plants to be lengthened and the number of lateral roots to be increased, so that the growth and development of the plants are promoted, and the growth and development of the plants are promoted. The biomass of the overground part is increased, and the function of the gene ZmESKIMO1 is lost, so that the primary root system of the plant is shortened, the number of lateral roots is reduced, and the biomass of the overground part is reduced. The invention finds and verifies that the corn ZmESKIMO1 gene has the function of regulating and controlling the growth and development of plants, and the discovery provides a new direction and theoretical support for the breeding of excellent corn varieties.

Constructs for expression of a gene product can comprise a circular RNA construct comprising sequence for encoding the gene product operably linked to an I RES-like sequence. The IRES-like sequence can be derived from a Hoxa gene or other developmental gene. Circular RNA constructs can be utilized to produce a gene product, treat an individual with a therapeutic gene product, or vaccinate an individual with an immunogenic gene product.