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Floral development genes

a technology of floral development and genes, applied in the field of plant molecular biology, can solve the problem of little information about how plants coordinate the activities

Inactive Publication Date: 2005-03-24
PIONEER HI BRED INT INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes new DNA segments containing instructions for making certain proteins. These protein-encoding genes can be found by comparing their genetic blueprints with other known genes. The patented genes include those responsible for producing factors involved in cell fate determination (FT), tumor formation (TFL) and apical polarity maintenance (Ap3). In addition, this patent includes the opposite strands of these genes which serve as controls. Overall, this technology allows researchers to better identify and study important components of biological systems.

Problems solved by technology

This patent discusses the identification and characterization of genes responsible for coordinating the development of reproductive organs in plants. Specifically, it focuses on the molecular mechanisms controlled by two families of regulatory genes called florial homeotic genes. By analyzing the genomics and bioinformatics data, researchers hope to identify new genes and gain insights into the complex network of interactions among them to improve crop yield and quality.

Method used

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Examples

Experimental program
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Effect test

example 1

Composition of cDNA Libraries; Isolation and Sequencing of cDNA Clones

[0089] cDNA libraries representing mRNAs from various Peruvian lily (Alstroemeria caryophylla), balsam pear (Momordica charantia), garden balsam (Impatiens balsamia), corn (Zea mays), rice (Oryza sativa), soybean (Glycine max), and wheat (Triticum aestivum) tissues were prepared. The characteristics of the libraries are described below. Corn developmental stages are explained in the publication “How a corn plant develops” from the Iowa State University Coop. Ext. Service Special Report No. 48 reprinted June 1993.

TABLE 2cDNA Libraries from Peruvian Lily, Balsam Pear, Garden Balsam,Corn, Rice, Soybean, and WheatLibraryTissueClonecbn10Corn Developing Kernel (Embryo and Endosperm);cbn10.pk0052.f510 Days After Pollinationcbn2Corn Developing Kernel Two Days After Pollinationcbn2.pk0035.f12cc71se-bCorn Callus Type II Tissue, Somatic Embryo Formedcc71se-b.pk0003.h10cco1nCorn Cob of 67 Day Old Plants Grown in Greencco1n...

example 2

Identification of cDNA Clones

[0095] cDNA clones encoding floral development proteins were identified by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410; see also the explanation of the BLAST alogarithm on the world wide web site for the National Center for Biotechnology Information at the National Library of Medicine of the National Institutes of Health) searches for similarity to sequences contained in the BLAST “nr” database (comprising all non-redundant GenBank CDS translations, sequences derived from the 3-dimensional structure Brookhaven Protein Data Bank, the last major release of the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). The cDNA sequences obtained in Example 1 were analyzed for similarity to all publicly available DNA sequences contained in the “nr” database using the BLASTN algorithm provided by the National Center for Biotechnology Information (NCBI). The DNA sequences were translated in all ...

example 3

Characterization of cDNA Clones Encoding Flowering Locus T (FT) Homologs

[0097] The BLASTX search using the EST sequences from clones listed in Table 3 revealed similarity of the polypeptides encoded by the cDNAs to FT and its homologs from Citrus unshiu (NCBI GenBank Identifier (GI) No. 4903139), Arabidopsis thaliana (NCBI GI Nos. 5002246 and 2190540), Oryza sativa (NCBI GI Nos. 5360178 and 5360180) and Nicotiana tabacum (NCBI GI No. 5453314). Shown in Table 3 are the BLAST results for individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), the sequences of contigs assembled from two or more ESTs (“Contig”), sequences of contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding an entire protein derived from an FIS, a contig, or an FIS and PCR fragment sequence (“CGS”):

TABLE 3BLAST Results for Sequences Encoding PolypeptidesHomologous to Flowering Locus T (FT) ProteinBLAST ResultsCloneStatusNCBI G...

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Abstract

This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.

Description

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Claims

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Application Information

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Owner PIONEER HI BRED INT INC
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