99 results about "Recombinant DNA Proteins" patented technology

The present disclosure relates to engineered RNA m6A demethylases with disrupted low complexity regions (LCRs) for expression in plants, as well as recombinant DNA encoding the engineered RNA m6A demethylases, vectors encoding the recombinant DNA, plants including the engineered RNA m6A demethylases, and method of improving the growth of plants using the engineered RNA m6A demethylases, including increased root growth and elevated photosynthesis. The present disclosure further relates to methods of improving growth of a plant, including increased root growth and elevated photosynthesis, by modifying endogenous ALKBH5 homolog genes to include disrupted LCRs, and plants produced by those methods.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The present invention belongs to the field of plant molecular biology and relates to the excision of recombinant DNA from the genome of plant somatic cells.

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and / or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The present disclosure relates to the Field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure aiso provides herbicide tolérant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molécules, and methods of using the same.

A method for improving the replication of covalent closed circular plasmids is provided. [Solution] The method comprises the step of preparing a covalent closed circular plasmid having a Pol I-dependent origin of replication and an insert containing a structured DNA sequence selected from the group consisting of a reverse repeat sequence, a directional repeat sequence, a homopolymer repeat sequence, a eukaryotic origin of replication, or a eukaryotic promoter enhancer sequence, wherein the structured DNA sequence is located at a distance of less than 1000 bp from the Pol I-dependent origin of replication in the direction of replication. The method also comprises the step of modifying the covalent closed circular recombinant molecule so that the Pol I-dependent origin of replication is replaced with a Pol III-dependent origin of replication, thereby improving the replication of the covalent closed circular plasmid with the resulting Pol III-dependent origin of replication. A covalent closed circular recombinant DNA molecule without antibiotic markers is also provided.

The invention relates to biotechnology and provides novel engineered protein for conferring enhanced growth. The invention also provides transgenic plants, seeds, cells and plant parts containing recombinant DNA molecules, as well as methods of using the same.

The invention discloses an hph resistance gene knockout box based on gme5 gene editing, a genetically engineered bacterium and application of the hph resistance gene knockout box and the genetically engineered bacterium. The hph resistance gene knockout box comprises a recombinant DNA construction body with the nucleotide sequence shown as SEQ ID NO: 1, the recombinant DNA construction body replaces an endogenous regulatory factor gme5 gene through homologous recombination in a targeted mode, and the nucleotide sequence of the regulatory factor gme5 gene is shown as SEQ ID NO: 2. According to the application, the bumblebee candida albicans are directionally modified through a genetic engineering strategy, an engineering strain for synthesizing the high-yield lactone type sophorolipid is constructed, and the lactonase activity and fatty acid transport capacity are synergistically enhanced, so that the ratio of the lactone type sophorolipid is maximized, and the yield of the lactone type sophorolipid is increased. In the sophorolipid produced by using the genetically engineered bacterium and the fermentation production method disclosed by the invention, the total content of the sophorolipid is 358.77 g / L, and the lactone content accounts for 74% or more of the total sophorolipid, so that the separation and purification difficulty and the production cost are greatly reduced, and an efficient and low-cost solution is provided for industrial fermentation production of the sophorolipid.

The invention discloses application of a tomato gene SlZFP2 as a negative regulation factor in regulation of plant cold resistance, belongs to the technical field of plant genetic engineering, and finds and confirms that the tomato zinc finger protein gene SlZFP2 is a key negative regulation factor of plant cold resistance for the first time. Experiments show that the expression of the SlZFP2 is induced by low temperature, the overexpressed transgenic tomato plant shows more serious cold injury symptoms under low temperature stress, the photosynthetic efficiency is reduced, active oxygen (H2O2 and O2-) is excessively accumulated, and the activity of antioxidant enzymes (SOD, POD and CAT) is weakened; the overexpression of the SlZFP2 can inhibit the biosynthesis and signal transduction of abscisic acid (ABA), and down-regulate the expression of cold response genes SlGRAS4 and SlCBF1; based on the discovery, the invention provides an application of using the SlZFP2 gene or an expression product thereof as a molecular marker for screening tomato materials with different cold resistance, creating a transgenic plant with reduced cold resistance by overexpressing the SlZFP2, and providing a recombinant DNA construct containing an SlZFP2 coding sequence and a host cell.

Provided are transgenic plants expressing MtDef5 antifungal proteins and peptides exhibiting high levels of resistance to susceptible fungi. Such transgenic plants contain a recombinant DNA construct comprising a natural or heterologous signal peptide sequence operably linked to a nucleic acid sequence encoding these molecules. Also provided are methods of producing such plants, methods of protecting plants against susceptible fungal infection and damage, as well as compositions that can be applied to the locus of plants, comprising microorganisms expressing these molecules, or these molecules themselves, as well as pharmaceutical compositions containing these molecules. Human and veterinary therapeutic use of MtDef5 antifungal proteins and peptides to treat susceptible fungal infections are also encompassed by the invention.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

The present disclosure relates to the field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molecules, and methods of using the same.

The present disclosure relates to novel methods and compositions for conferring glyphosate tolerance to plants. The disclosure also provides glyphosate tolerant plants, seeds, tissues, cells and plant parts comprising the modified EPSP synthase and a recombinant DNA molecule encoding the modified EPSP synthase, as well as methods of producing the glyphosate tolerant plants, seeds, tissues, cells and plant parts and uses thereof.

The invention discloses a preparation method of high-purity TIR2 and T1R3 recombinant plasmids. The preparation method comprises the following steps: transforming the T1R2 and T1R3 recombinant plasmids into modified Trans1-T1 escherichia coli competent cells through a heat shock method; large-scale amplification of plasmids is carried out by utilizing the efficient cloning characteristic of Trans1-T1 escherichia coli; and extracting and purifying recombinant plasmids from thalli by adopting an optimized alkali cracking combined column purification technology. According to the preparation method of the high-purity TIR2 and T1R3 recombinant plasmids, target DNA fragments or recombinant DNA plasmids constructed by the target DNA fragments are introduced into competent cells, amplification of genetic materials is achieved through a replication and expression system of host cells, finally the high-purity DNA plasmids are obtained through extraction and purification, and the high-purity TIR2 and T1R3 recombinant plasmids are obtained by improving transformation, amplification and purification processes. The yield and the purity of the T1R2 / T1R3 recombinant plasmid are remarkably improved, and the high-standard requirement of a transfection experiment is met.

This invention discloses a targeted adjuvant influenza vaccine composition for atrial fibrillation patients with atrial fibrosis. The vaccine antigen components are selected from the surface antigens of currently prevalent influenza virus strains, such as hemagglutinin (HA) and neuraminidase (NA). High-purity influenza virus antigens are prepared through cell culture or recombinant DNA technology. For example, influenza virus is cultured using Madin-Darby canine kidney (MDCK) cells, and purified through centrifugation, filtration, and chromatography to obtain high-purity HA and NA antigens. After injection into the human body, the influenza virus antigens (HA and NA) are taken up and processed by antigen-presenting cells (such as macrophages and dendritic cells). The antigen-presenting cells present antigen peptides to T lymphocytes and B lymphocytes, activating a specific immune response. B lymphocytes differentiate into plasma cells with the help of T lymphocytes, producing specific antibodies that recognize and bind to the influenza virus, preventing it from infecting host cells and thus preventing influenza virus infection.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

The present invention provides recombinant DNA molecules and constructs useful for modulating gene expression in plants, as well as nucleotide sequences thereof. The present invention also provides transgenic plants, plant cells, plant parts, and seeds comprising a recombinant DNA molecule comprising a DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of use thereof.

The invention provides recombinant DNA polynucleotides and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides operably linked to heterologous transcribable DNA polynucleotides. Also provided are methods of the use of the recombinant DNA polynucleotides and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides and constructs.

The invention relates to a recombinant hybrid protein REC2z with a structure as defined in the description, recombinant DNA sequences encoding this protein, vectors containing the recombinant DNA sequences, and eukaryotic and prokaryotic expression systems using the in vitro recombinant DNA sequences described below. The invention also relates to the use of the recombinant hybrid protein REC2z as a component of bioink for bioprinting and also as a component of biomaterials. In addition, the invention relates to the use of recombinant DNA sequences, expression vectors and expression systems to produce the recombinant hybrid protein REC2z. The invention finds applications in bioprinting, medicine and cell and tissue engineering.

The invention belongs to the technical field of in-vitro nucleic acid detection, and discloses a pyrosequencing primer and a kit for detecting CYP2D6-5 genetic typing. And the base sequence of the pyrosequencing primer is as shown in SEQ NO.3. The kit comprises dNTPs, a buffer solution, a recombinant DNA polymerase (rTaq), double distilled water, a specific amplification primer 1, a specific amplification primer 2, a sequencing primer, a blank reference substance and a positive reference substance, the base sequence of the specific amplification primer 1 is as shown in SEQ NO.1; the base sequence of the specific amplification primer 2 is as shown in SEQ NO. 2; the base sequence of the sequencing primer is as shown in SEQ NO.3. When the kit is used for detecting the CYP2D6 * 5 genotyping, the kit has the advantages of accurate qualitative detection, high sensitivity and strong specificity; in addition, the method has the advantages that sample treatment is simple, sequencing steps are simple, the sequencing speed is high, one on-machine reaction is completed in half an hour, detection site frequency analysis is directly given, and the result is visual.

The invention relates to novel methods and compositions for conferring tolerance to glyphosate to plants. The invention also provides glyphosate-tolerant plants, seeds, tissue, cells, and plant parts comprising modified EPSP synthases and recombinant DNA molecules encoding modified EPSP synthases, as well as methods of producing the same and the use thereof.

The present disclosure provides compositions, methods of use, and methods of creation for a population of transgenic plants derived from plant cells transformed with recombinant DNA for expression of heterologous proteins. In particular, the present disclosure provides compositions comprising indoor ornamental plants suited for the removal of volatile organic compounds such as formaldehyde, benzene, toluene, ethylbenzene and / or xylene from air. Also disclosed are transgenic seeds for growing a transgenic plant having the recombinant DNA in its genome and exhibiting enhanced VOC removal from air. Also disclosed are methods for generating seed and plants based on the transgenic events. Also disclosed are microbes selected for during directed evolution to have enhanced VOC removal from air capabilities. Also disclosed are methods and compositions for generating plant-microbiome pairings for enhanced VOC removal from air.

The present invention relates to recombinant DNA technology, in particular to methods for assembling two or more double stranded (ds) nucleic acid molecules with overlapping terminal sequences. In particular, the present invention relates to the use of a thermolabile DNA polymerase II derived 3′-5′ exonuclease isolated from Moritella viscoa and a thermolabile DNA polymerase I of marine origin in multi DNA assembly processes.

The invention provides recombinant DNA molecules that are unique to maize event MON87429 and transgenic maize plants, maize plant parts, maize seeds, maize cells, and agricultural products containing maize event MON87429 as well as methods of using and detecting maize event MON87429. Transgenic maize plants containing maize event MON87429 exhibit tolerance to inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group, synthetic auxins, inhibitors of glutamine synthetase, and inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

The invention described herein provides an improved system and method for rAAV production. The system of the invention includes a single recombinant DNA vector that comprises a gene of interest (GO I) to be delivered to a target cell, the Rep and Cap coding sequences, as well as the Helper gene coding sequences for rAAV packaging.

The application belongs to the technical field of molecular biology, and discloses a recombinant DNA polymerase, a preparation method, a kit and application in probe qPCR amplification. The recombinant DNA polymerase provided by the application comprises an amino acid fragment as shown in SEQ ID NO: 1 and / or a variant sequence with more than 90 % homology with the amino acid fragment shown in SEQ ID NO: 1. The recombinant DNA polymerase has excellent anti-inhibition ability and thermal stability, can be well applied to probe qPCR to realize qualitative and quantitative detection of a target sequence in a sample to be detected, especially has good detection effect on complex samples, and has important significance in the application in the fields of clinical diagnosis, food safety detection and the like.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.