76results about How to "Improve metabolic efficiency" patented technology

The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2 / 3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2 / 0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2 / 0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.

The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2 / 3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2 / 0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2 / 0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.

A chewing gum with a liquid-fill composition and a surrounding gum region, with one or both containing active ingredients, such as herbal, medicinal and / or mineral elements or combinations thereof, present in an amount of at least about 0.05 mg.

The invention discloses an aquaculture water quality regulation system disposed in closed water. The system includes: a gas input unit and a floating bed plant unit. The gas input unit is arranged on the surface of the closed water, and is used for conveying gas-liquid dispersions into the closed water and providing water flow power to the closed water. The floating bed plant unit is suspended at the surface for the closed water to absorb nitrogen and phosphorus in the closed water. The invention also discloses an aquaculture water quality control method, which can provide a good water environment for aquaculture of shrimps, fish, crabs and the like, and also can makes use of the excreta of shrimps, fish, crabs and the like to produce green organic vegetables. The system and the method provided in the invention not only provide a good water environment for aquaculture, improve the yield and quality of aquatic products, but also can produce large amounts of green organic vegetables, thus realizing effective utilization of resources.

The invention relates to a goat milk kefir produced with polymerized lactalbumin as a thickener, and belongs to the technical field of functional foods. The goat milk kefir is produced through coordinating fresh goat milk as liquid raw milk with sugar, polymerized lactalbumin, low methoxyl pectin and a kefir starter. The goat milk kefir product is produced through the following steps: mixing sugar with low methoxyl pectin in advance, adding the obtained mixture to the liquid raw milk; adding a polymerized lactalbumin solution; stirring and heating the above solution to 80-90DEG C, carrying out heat insulation for 10-20min for sterilization, and carrying out ice bath cooling to room temperature; and adding the kefir starter, stirring above materials until uniformity, and fermenting the obtained mixture at 25-35DEG C for 8-14h. The product has the advantages of high content of proteins, low content of fats, stable curd state, uniform structure, moderate viscosity, unique flavor and various health functions; and a production technology of the goat milk kefir has the advantages of few raw material components, easy operation, energy saving and low cost, and is a simple production processing mode.

The invention discloses magnetic sludge carbon biological filler with a core-at-shell structure and preparation method and application thereof. The magnetic sludge carbon biological filler is prepared by using Fe3O4 magnetic microsphere as a magnetic core, iron-containing sludge as an intermediate layer raw material and common sludge or zinc-containing sludge (preferred) as a shell raw material, adopting a repetitive impregnation-drying method to sequentially wrap the intermediate layer raw material and the shell raw material on the Fe3O4 magnetic microsphere to form a precursor of the core-at-shell structure, and then performing pyrolysis and after-treatment. Since the magnetic material is used as the core and the sludge carbon is used as the shell material, the prepared magnetic sludge carbon biological filler with the core-at-shell structure not only can solve the problem that a powder magnetic material is difficult to separate, and can also solve the problem that ferrite used as a magnetic matrix is corroded by microorganisms; the specific surface of the porous sludge carbon in a shell layer is large, a magnetic environment for growth can be provided for more microorganisms, the metabolic efficiency of the microorganisms is improved and the tail water treatment efficiency of domestic sewage plants is greatly improved.

The invention discloses an anaerobic fermentation technology for rural kitchen waste. The anaerobic fermentation technology for the rural kitchen waste comprises the following steps of (1) sorting and impurities removing, (2) flocculation and filtration, (3) extraction and concentration, and (4) anaerobic fermentation. According to the anaerobic fermentation technology for the rural kitchen waste, the yield of metabolite in the acid producing stage can be increased effectively so that the yield of methane and the bioavailability of the rural kitchen waste can be increased.

The invention relates to a special compound feed for quick growth period of trepang and a making method of the special compound feed, and belongs to the technical field of breeding of aquatic products. The special compound feed comprises the raw material components of 35-50 parts of fermented soybean meal, 5-10 parts of white fish meal, 15-20 parts of algae powder, 5-10 parts of shrimp powder, 0-10 parts of glucose, 0-10 parts of brown sugar, 2-4 parts of lecithin, 2-4 parts of a vitamin premix, 2-4 parts of a mineral element premix, 2-4 parts of a probiotic premix, 2-4 parts of an enzyme preparation premix, 2-4 parts of calcium dihydrogen phosphate, 8-12 parts of guar gum and 200-300 parts of sea mud. The making technology comprises the following steps of (1) preparing the raw materials;(2) performing weighing and mixing; (3) performing crushing; (4) performing secondary weighing and mixing; (5) performing compounding; (6) performing fermentation; (7) performing extruding; and (8) performing drying. According to the special compound feed disclosed by the invention, the utilization rate of nutrients and energy of the feed can be increased, the fermentation technological processesare simplified, the palatability and the nutrient usability of the feed are good, the viscoelastic properties are high, the dissolving rate of the feed in water is low, and the feed alleviates pollution generated during breeding and can improve the immunity of organisms.

The invention discloses a novel sewage tank. Enough oxygen is provided for a microorganism through generating continuous micro-bubbles at the bottom of a tank body; a carrier for apposition growth is provided for the microorganism through a biological active filling; the metabolic efficiency of the microorganism is enhanced; meanwhile, sewage forms up-and-down convection in the tank body under the actions of a gravity current and the micro-bubbles to generate agitation; dirt cannot precipitate; the suspended carrier is also enabled to migrate and spread all over in the tank along with a water current; an agent is uniformly mixed; the purpose of purification treatment is effectively achieved.

The invention relates to a plant expression vector pH2-35S-PrbcS-adh of arabidopsis glutathione-dependent formaldehyde dehydrogenase gene, which is the plant expression vector of light-induced promoter (PrbcS) containing tomato Rubisco 3C small subunit gene and the arabidopsis glutathione-dependent formaldehyde dehydrogenase (FALDH) gene adh. The gene adh is amplified from arabidopsis, and the over expression of the gene adh in tobacco lamina cytoplasm is controlled by the light-induced promoter, so that the efficiency of decomposing formaldehyde by tobacco cell can be improved, thus enhancing the capability of absorbing and enduring exogenous formaldehyde by plant. The experiment proves that enzyme activity of adh transgenic tobacco FALDH is increased by about 3-5 times compared with that of wild type; after being cultured for 30 days on an MS solid culture medium containing 10mmol / L of formaldehyde, the growing situation of the adh transgenic tobacco is obviously superior to that of wild type. In addition, when 2mM formaldehyde liquid is used for treating the plant, the formaldehyde absorption rate of the adh transgenic tobacco is obviously higher than that of wild type.

The invention discloses soymilk with an original flavor. The soymilk is prepared from raw materials with the following parts by weight: 90 to 100 parts of soybeans, 70 to 80 parts of white granulated sugar, 8 to 10 parts of coconut oil, 3 to 4 parts of a stabilizer, 0.1 to 0.2 part of D-sodium erythorbate, 0.3 to 0.4 part of sodium tripolyphosphate, 0.2 to 0.3 part of sodium citrate, 0.04 to 0.05 part of ethyl maltol, 0.3 to 0.4 part of table salt, and 0.7 to 0.8 part of baking soda. A preparation method comprises the following steps: a. peeling; b. soaking; c. soybean grinding; d. primary filtration; e. refined filtration; f. soymilk boiling and enzyme deactivation; g. volume fixing of the ingredients; h. homogenization; i. filling; j. sterilization.

The invention belongs to the field of wastewater treatment, and particularly relates to a method for promoting formation of anaerobic granular sludge, which comprises the following steps: 1) adding an iron-based material into an anaerobic reactor, and adding sludge; 2) pumping synthetic wastewater into the anaerobic reactor, and starting the anaerobic reactor; and 3) when gas production and water outgoing are stable in the reaction, and the measured volatile suspended matter of the granular sludge accounts for 70-90% of the total suspended matter mass of the sludge, determining that the granular sludge is mature, stopping running of the reactor, and recovering mature granular sludge. The iron-based material added in the invention has a large specific surface area, which is beneficial to enrichment of anaerobic microorganisms on the surface of the iron-based material so as to form a sludge aggregate. The iron-based material is close to mature granular sludge in density and can float up and down in the anaerobic reactor, so that sludge deposition caused by increase of the volume weight of the sludge is avoided. The iron-based material is used as a core, so that the mechanical property of the anaerobic granular sludge is improved.

The invention discloses a chufa polysaccharide. The chufa polysaccharide consists of the following components in mol contents: 0.71% of mannose, 5% to 16% of rhamnose, 0.71% of galacturonic acid, 76.74% to 83.82% of glucose, 3.48% to 8.51% of galactose and 1.25% to 2.36% of arabinose. The Mn (number-average molecular weight) of the chufa polysaccharide is 1.116*10<7> to 1.137*10<7>, the Mw (weight-average molecular weight) of the chufa polysaccharide is 1.461*10<7> to 1.618*10<7>, and the polydispersity index of the chufa polysaccharide is 1.286 to 1.434. The chufa polysaccharide has better effects of preventing alcoholism and protecting the liver.

The invention discloses an anaerobic fermentation method for straw. The anaerobic fermentation method for the straw comprises the following steps that (1) sulfite is adopted to pretreat straw powder; (2) the pretreated straw powder is subjected to ultrasonic enzymolysis; (3) then the straw powder is soaked by using a sodium hydroxide solution; (4) the straw powder and the solution are separated, and acidogenic fermentation is conducted correspondingly; and (5) finally, methane-producing fermentation process is conducted. According to the anaerobic fermentation method for the straw, the yield of metabolite in the acid producing stage can be increased effectively so that the yield of methane and the use ratio of the straw can be increased.

The invention relates to the technical field of milk tea beverages, and provides a plant-based milk tea beverage and a preparation method aiming at the current situation that milk tea on the market ismilk-based milk tea and is lack of a full-plant-based milk tea beverage without animal milk fat. The beverage is prepared from the following components: a plant base material, a tea raw material, a coffee raw material, a stabilizer, a fat base material, an emulsifier, an antioxidant, nisin, an acidity regulator, a sweet component, nuts and the like; the plant base material is one or more of hulled rice or powder and coconut milk of cereal crop fruits; and the fat base material is one or more of coconut powder, coconut oil, medium-chain triglyceride and sunflower seed oil. The pursuit of current consumers for the health concept is met, compared with milk-based milk tea or milk-based milk tea containing plant materials on the market, the concept of whole-plant-based milk tea is achieved, and the consumption requirements for being healthier, more nutritional and unique in taste are met.

A chewing gum with a liquid-fill composition and a surrounding gum region, with one or both containing active ingredients, such as herbal, medicinal and / or mineral elements or combinations thereof, present in an amount of at least about 0.05 mg.

The invention relates to the technical field of environmental microorganisms, and particularly relates to an aromatic hydrocarbon degrading enzyme preparation and a preparation method as well as application thereof. The enzyme preparation is prepared by steps of fermenting and culturing the indigenous microorganism with an aromatic hydrocarbon degradation function, performing ultrasonication and extracting intracellular mixture, and further performing immobilization to form the enzyme preparation, wherein the indigenous microorganism with the aromatic hydrocarbon degradation function is pseudomonas fluorescens collected in the China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC NO: M2011260 on July 22, 2011. In application, the enzyme preparation provided by the invention is efficient, fast, and free of secondary pollution relative to bacteria, so that a new biological remediation way and thinking is provided for treating the environment pollution caused by organic pollutants.

The invention belongs to the technical field of drug cultivation, and in particular relates to a drought-resistant cultivation method of codonopsis pilosula. The method is carried out according to thefollowing steps: step 1, land selection and soil preparation; step 2, fertilization; step 3, seedling selection; step 4, transplanting method; step 5, field management, step 6, disease and pest control; step 7, excavation; and step 8, storage. According to the drought-resistant cultivation method of the codonopsis pilosula, temperature can be increased and soil moisture can be maintained, therebyfacilitating yield improvement of medicinal materials; natural resources of light, heat, water, soil, etc. are fully utilized, so that Chinese herbal medicines grow vigorously, and resilience and pest and disease resistance are further enhanced, therefore, occurrence variety and incidence rate of diseases and insect pests are correspondingly reduced, use number and use amount of pesticides are reduced correspondingly, root length and root diameter of the codonopsis pilosula can be significantly improved, thereby increasing a ratio of first-class products, and improving commercial characters of the medicinal materials.

The invention belongs to the technical field of human health, and particularly discloses an edible compound recipe mitochondrial energy enhancing formula.The edible compound recipe mitochondrial energy enhancing formula comprises the components of pyrroloquinoline quinone, coenzyme Q10, nicotinamide mononucleotide, mecobalamin, calcium L-5-Methyltetrahydrofolate and complex vitamins B. The ediblecompound recipe mitochondrial energy enhancing formula is prepared through the steps of mixing the pyrroloquinoline quinone with the coenzyme Q10, the nicotinamide mononucleotide, the mecobalamin, thecalcium L-5-Methyltetrahydrofolate and the complex vitamins B to obtain finished products of the enhancing formula, and adding synergistic reaction components for NAD on-line plastochondria respiratory chain complexes and components for strengthening mitochondrial biogenesis, so that the respiratory chain metabolism efficiency is comprehensively improved, and the problem that a NAD precursor is separately supplemented, and the effect of improving vigour is not achieved, is solved. The components for assisting in NAM metabolism and discharge are added, so that the organism metabolism NAM pressure is reduced, and the potential risk that the NAD precursor is taken for a long time can be reduced.

The invention belongs to the field of health care food, and discloses a method for reusing mulberry pomace. According to the method, mulberry pomace powder is prepared by reusing the mulberry pomace and is applied to the preparation of vegetable noodles for regulating blood vessel health; the quality and effects of the vegetable noodles are improved by means of the active ingredients and nutrientsin the mulberry pomace powder, so that the vegetable noodles not only can supplement rich protein, mineral elements and other nutrients to a human body, but also effectively clean the intestines andcondition the cardiovascular system, and further better improve the human health on the whole; in addition, the mulberry flavor of the mulberry pomace powder can further improve the quality of the vegetable noodles; the vegetable noodles are enabled to have the mulberry fruit flavor, so that the taste of the vegetable noodles is improved.

The invention discloses a full-water-soluble organic-inorganic compound fertilizer, which comprises potassium fulvate, ammonium sulfate, monopotassium phosphate, anhydrous magnesium sulfate, metalloproteinase and Jikang enzyme. The Jikang enzyme is prepared by the following steps: uniformly stirring and mixing 100 parts of plant acid, 600 parts of amino acid and 5 parts of an amino acid surfactant; adding 100 parts of mineral source fulvic acid and 150 parts of humic acid, fully stirring and uniformly mixing all the components, adding the mixture into a fermentation tank, inoculating a microbial agent for cultivation for 18 hours, and finally adding 15 parts of chitosan and 10 parts of subtilisin, and uniformly mixing all the components for obtaining the Jikang enzyme. The invention also discloses a preparation method of the fertilizer, which comprises the following steps: preparing metalloproteinase, and mixing the raw materials. The compound fertilizer is a biological, organic and inorganic combined fertilizer, organic components (fulvic acid) in the fertilizer form colloidal substances in soil, the formation of a soil aggregate structure can be effectively promoted, and the water, fertilizer, gas and heat conditions of the soil are coordinated.

The invention discloses a premix for high-yield laying hens and a production method thereof. The premix per ton contains 120 to 200 kg of stone powder, 160 to 220 kg of calcium hydrogen phosphate, 60to 65 kg of edible salt, 35 to 45 kg of a compound trace element, 5 to 7 kg of a compound vitamin, 2 to 3 kg of vitamin C, 0.1 to 0.3 kg of 25-hydroxy vitamin D3, 30 to 36 kg of choline chloride, 20 to 30 kg of anhydrous sodium sulphate, 25 to 35 kg of methionine, 20 to 30 kg of lysine sulfate, 0.8 to 1 kg of ethoxyquinoline, 1 to 2 kg of pediococcus acidilactici, 2 to 4 kg of phytase, 2 to 4 kg of a poultry compound enzyme, 5 to 10 kg of a compound traditional Chinese herbal medicine, 50 to 300 kg of medical stone and 50 to 150 kg of rice hull powder. The premix is prepared by mixing all components. According to the invention, the production process is simple; the egg laying rate is increased; the egg quality is improved; and the death and culling rate and the egg breaking rate are reduced.

The invention relates to rice milk and a preparation method thereof, belonging to the field of functional health-care food. The rice milk comprises the following main components in parts by weight: 1-20 parts of rice powder, 0-10 parts of sugar, 0.1-10 parts of concentrated whey protein or / and soy isolate protein, 0-2 parts of inulin, 0.1-0.5 part of glycerin monostearate, 0.1-2 parts of plant oil, and 100 parts of water; and the rice milk further comprises vitamin and a mineral nutrition enhancer. The preparation method comprises the following steps of preparing rice as the main raw material into rice slurry or rice powder dispersion liquid, adding other components, and grinding, homogenizing, heating and quickly cooling. The rice milk disclosed by the invention adopts a novel form, and is convenient to carry, simple and convenient to eat, abundant in nutritional substance, high in protein content, low in fat content, smooth, fine, good in mouth feel, refreshing in fragrance and the like. According to the preparation method, the operation is simple, the cost is low and no pollution exists, and the method is provided for producing steady and uniform rice milk.

The invention discloses a traditional Chinese medicine composition, and particularly relates to a traditional Chinese medicine composition for blacking hair. The traditional Chinese medicine composition for blacking hair comprises a front dressing and a rear dressing. The front dressing is a paste and is composed of goose barnacles and jujube pits. The rear dressing is also a paste and is composed of black sesame, radix polygni multiflori, fructus chebulae, fermented Chinese gall herb and tea leaves, fructus caryophylli, fructus gardeniae and lysozyme. The traditional Chinese medicine composition for blacking hair overcomes the defect of hair dye in the prior art that toxic and side effects are serious, and has small toxic and side effects on the human body.

The invention provides an ultrasound and microbe cooperated wastewater treatment device which comprises a reactor container. A partition plate is arranged in the reactor container and divides the reactor container into a first cavity and a second cavity. A wastewater inlet pipe is arranged at the upper end of the first cavity, and a valve is arranged on a pipe body of the wastewater inlet pipe. A heating bar and a temperature control sensor are arranged in the second cavity, a wastewater discharging pipe and a peristaltic pump water inlet are arranged on the lower side of the second cavity, and the inner pipe orifice of the wastewater inlet pipe is sleeved with a filter bag. The peristaltic pump water inlet is connected with a peristaltic pump, an ultrasound irradiation chamber and a water outlet in sequence through pipes, and the water outlet is formed in the upper end of the first cavity. An intelligent pulse control unit is arranged above the ultrasound irradiation chamber, a plurality of ultrasound heads arranged in an array mode are arranged under the ultrasound irradiation chamber, and the intelligent pulse control unit is connected with the ultrasound heads. A plurality of aerator pipes are arranged at the bottom inside the reactor container. The intelligent pulse unit is added in the device, so that the working time and frequency are intelligently controlled, the device cyclically treats wastewater, and the cost is reduced.